Rroxscaffold_6G00423070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002282
Physical Location & Seq
Forward (+)
43998694 .. 43999612
919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00423070.1

Sequence Viewer

Length: 555 bp
ATGGTAGGTCCCTCCTACAATATGCATCCGGTAGACCGACACCCGAGCTACCTCGCCATGGTGGAGGTCGGCCGATATCTTGCCAGGAGGCCACCCTCCCATGCTCTTCAAAAGCCTTCAAGAGAAAAGGCAAAAATCCCTCATTCTACACGGGGCCACGCCACCGGGTACCGGGAGGCCAAAGCCACCGGTACCGGAGGCCAAAGCCACCGAACACCCCAACGGGGTTACATCATTCTACCCGAGGCCATGCCACCGTGTAAAGGAGGCCTACCGCCCTCATTCAACCCCATTGGGTTAAGGAGGCTCAAGACCTCATCTATTCCACCGGGATCACGGCTCACTCACCTCATAGGGTACATGAGGCCACGAGCCTATCTACTGAGGCCAAGGTCTAGGTATCCGAGTCGGCCCCAACCTCATCGGGTACCGAGAGGCCAAGGCCATAAATACCCTATCGGGTACACGAGATTCCTAGCTCTCAAATCCCAACGGGATCACCGGTTCACTCACCTCATCGGGTACATGAGGCCACGAGCCTATCTACTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.96

Weight (kDa)

11.47

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 3 cut(s) 168, 191, 427
AccB1I GGYRCC 3 cut(s) 168, 191, 427
AccI GTMKAC 1 cut(s) 33
AciI CCGC 1 cut(s) 275
AclWI GGATC 2 cut(s) 340, 504
AcoI YGGCCR 1 cut(s) 70
AfaI GTAC 6 cut(s) 170, 193, 359, 429, 464, 524
AfiI CCNNNNNNNGG 4 cut(s) 58, 171, 224, 263
AgeI ACCGGT 2 cut(s) 188, 501
AgsI TTSAA 3 cut(s) 110, 120, 286
AjnI CCWGG 1 cut(s) 83
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
AluBI AGCT 2 cut(s) 48, 479
AluI AGCT 2 cut(s) 48, 479
AlwI GGATC 2 cut(s) 340, 504
Ama87I CYCGRG 2 cut(s) 43, 242
AsiGI ACCGGT 2 cut(s) 188, 501
Asp718I GGTACC 3 cut(s) 168, 191, 427
AspS9I GGNCC 3 cut(s) 8, 154, 411
AsuC2I CCSGG 3 cut(s) 166, 173, 330
AsuHPI GGTGA 3 cut(s) 338, 491, 503
AvaI CYCGRG 2 cut(s) 43, 242
AvaII GGWCC 1 cut(s) 8
BanI GGYRCC 3 cut(s) 168, 191, 427
BauI CACGAG 3 cut(s) 369, 466, 534
BceAI ACGGC 1 cut(s) 353
BciT130I CCWGG 1 cut(s) 85
BciVI GTATCC 1 cut(s) 411
BcnI CCSGG 3 cut(s) 166, 173, 330
BfaI CTAG 3 cut(s) 396, 476, 553
BfuI GTATCC 1 cut(s) 411
Bme1390I CCNGG 4 cut(s) 85, 166, 173, 330
Bme18I GGWCC 1 cut(s) 8
BmeT110I CYCGRG 2 cut(s) 43, 242
BmgT120I GGNCC 3 cut(s) 8, 154, 411
BmiI GGNNCC 6 cut(s) 10, 155, 170, 193, 413, 429
BmrFI CCNGG 4 cut(s) 85, 166, 173, 330
BmsI GCATC 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 3 cut(s) 166, 173, 330
BsaJI CCNNGG 4 cut(s) 57, 243, 389, 439
BsaWI WCCGGW 4 cut(s) 28, 188, 194, 501
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 171, 224, 263
Bse118I RCCGGY 2 cut(s) 188, 501
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 4 cut(s) 57, 243, 389, 439
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 4 cut(s) 58, 171, 224, 263
BseMII CTCAG 1 cut(s) 374
BseX3I CGGCCG 1 cut(s) 70
Bsh1285I CGRYCG 1 cut(s) 73
BshNI GGYRCC 3 cut(s) 168, 191, 427
BshTI ACCGGT 2 cut(s) 188, 501
BsiEI CGRYCG 1 cut(s) 73
BsiHKCI CYCGRG 2 cut(s) 43, 242
BsiSI CCGG 7 cut(s) 29, 165, 172, 189, 195, 329, 502
BslI CCNNNNNNNGG 4 cut(s) 58, 171, 224, 263
BsoBI CYCGRG 2 cut(s) 43, 242
Bsp143I GATC 2 cut(s) 332, 496
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 375
BspLI GGNNCC 6 cut(s) 10, 155, 170, 193, 413, 429
BspPI GGATC 2 cut(s) 340, 504
BspQI GCTCTTC 1 cut(s) 111
BspT107I GGYRCC 3 cut(s) 168, 191, 427
BsrFI RCCGGY 2 cut(s) 188, 501
BssAI RCCGGY 2 cut(s) 188, 501
BssECI CCNNGG 4 cut(s) 57, 243, 389, 439
BssMI GATC 2 cut(s) 332, 496
BssSI CACGAG 3 cut(s) 369, 466, 534
BssT1I CCWWGG 3 cut(s) 57, 389, 439
Bst2BI CACGAG 3 cut(s) 369, 466, 534
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 111
BstDEI CTNAG 1 cut(s) 383
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 2 cut(s) 335, 499
BstMBI GATC 2 cut(s) 332, 496
BstMCI CGRYCG 1 cut(s) 73
BstNI CCWGG 1 cut(s) 85
BstSCI CCNGG 4 cut(s) 83, 164, 171, 328
BstZI CGGCCG 1 cut(s) 70
BsuI GTATCC 1 cut(s) 411
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 25
Cfr10I RCCGGY 2 cut(s) 188, 501
Cfr13I GGNCC 3 cut(s) 8, 154, 411
Csp6I GTAC 6 cut(s) 169, 192, 358, 428, 463, 523
CspAI ACCGGT 2 cut(s) 188, 501
CviAII CATG 5 cut(s) 58, 101, 250, 361, 526
CviQI GTAC 6 cut(s) 169, 192, 358, 428, 463, 523
DdeI CTNAG 1 cut(s) 383
DpnI GATC 2 cut(s) 334, 498
DpnII GATC 2 cut(s) 332, 496
EaeI YGGCCR 1 cut(s) 70
EagI CGGCCG 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EclXI CGGCCG 1 cut(s) 70
Eco130I CCWWGG 3 cut(s) 57, 389, 439
Eco147I AGGCCT 1 cut(s) 270
Eco32I GATATC 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 8
Eco52I CGGCCG 1 cut(s) 70
Eco88I CYCGRG 2 cut(s) 43, 242
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 83
EcoRV GATATC 1 cut(s) 77
EcoT14I CCWWGG 3 cut(s) 57, 389, 439
EcoT22I ATGCAT 1 cut(s) 27
ErhI CCWWGG 3 cut(s) 57, 389, 439
FaeI CATG 5 cut(s) 61, 104, 253, 364, 529
FaiI YATR 8 cut(s) 23, 59, 102, 251, 353, 362, 447, 527
FatI CATG 5 cut(s) 57, 100, 249, 360, 525
FblI GTMKAC 1 cut(s) 33
FokI GGATG 1 cut(s) 12
FspBI CTAG 3 cut(s) 396, 476, 553
HapII CCGG 7 cut(s) 29, 165, 172, 189, 195, 329, 502
Hin1II CATG 5 cut(s) 61, 104, 253, 364, 529
HinfI GANTC 2 cut(s) 406, 471
HpaII CCGG 7 cut(s) 29, 165, 172, 189, 195, 329, 502
HphI GGTGA 3 cut(s) 338, 491, 503
Hpy166II GTNNAC 3 cut(s) 34, 465, 507
Hpy188I TCNGA 1 cut(s) 405
Hpy188III TCNNGA 2 cut(s) 120, 310
Hpy8I GTNNAC 3 cut(s) 34, 465, 507
HpyAV CCTTC 1 cut(s) 126
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4V TGCA 1 cut(s) 25
HpyF3I CTNAG 1 cut(s) 383
Hsp92II CATG 5 cut(s) 61, 104, 253, 364, 529
KpnI GGTACC 3 cut(s) 172, 195, 431
Kzo9I GATC 2 cut(s) 332, 496
LguI GCTCTTC 1 cut(s) 111
LpnPI CCDG 9 cut(s) 42, 70, 97, 178, 185, 202, 208, 342, 515
LweI GCATC 1 cut(s) 34
MaeI CTAG 3 cut(s) 396, 476, 553
MaeIII GTNAC 1 cut(s) 227
MalI GATC 2 cut(s) 334, 498
MboI GATC 2 cut(s) 332, 496
MboII GAAGA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 415
Mph1103I ATGCAT 1 cut(s) 27
MseI TTAA 1 cut(s) 299
MspI CCGG 7 cut(s) 29, 165, 172, 189, 195, 329, 502
MspR9I CCNGG 4 cut(s) 85, 166, 173, 330
MvaI CCWGG 1 cut(s) 85
NciI CCSGG 3 cut(s) 166, 173, 330
NcoI CCATGG 1 cut(s) 57
NdeII GATC 2 cut(s) 332, 496
NlaIII CATG 5 cut(s) 61, 104, 253, 364, 529
NlaIV GGNNCC 6 cut(s) 10, 155, 170, 193, 413, 429
NsiI ATGCAT 1 cut(s) 27
PceI AGGCCT 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 111
PfeI GAWTC 1 cut(s) 471
PinAI ACCGGT 2 cut(s) 188, 501
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 6 cut(s) 10, 155, 170, 193, 413, 429
PspPI GGNCC 3 cut(s) 8, 154, 411
PspPPI RGGWCCY 1 cut(s) 8
RsaI GTAC 6 cut(s) 170, 193, 359, 429, 464, 524
RsaNI GTAC 6 cut(s) 169, 192, 358, 428, 463, 523
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 299
Sau3AI GATC 2 cut(s) 332, 496
Sau96I GGNCC 3 cut(s) 8, 154, 411
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 4 cut(s) 85, 166, 173, 330
SfaNI GCATC 1 cut(s) 34
SinI GGWCC 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 1 cut(s) 275
SspMI CTAG 3 cut(s) 396, 476, 553
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 4 cut(s) 83, 164, 171, 328
StyI CCWWGG 3 cut(s) 57, 389, 439
TaaI ACNGT 1 cut(s) 258
TaqII GACCGA 1 cut(s) 51
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 3 cut(s) 396, 476, 553
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.