Rroxscaffold_1G00053470

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001171
Physical Location & Seq
Forward (+)
74424255 .. 74427360
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053470.1

Sequence Viewer

Length: 1320 bp
ATGTTGGGCACAGTGAAGGCGCGTTGGGTGTTCGCGACAGTGGTTTTTTTCTCGGTGGAGGTTTCGTGGAGCAAACCGAGAGTTGAGTTGGGGAGAGAGAAGCCTTTTCTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCGCCGGCGAAGTCGTCGTCGTCGTCCGAAGAGCCACTGGTCAGTCTGGTCTCTATGGCTTCTAGCGACGGAGGCCAAGCAGAGTTCTACACCGAAACGACGACGGATAAGGGGTCGGGGAATCTGGAGTACGATTTCTATCGGAACTCGTGTCCTCAGGCAGAGACCATTGTGAGGTCGAAGATGGCTTGGATTCACGGCCAGCACAGGAACGTCTCGGCCCAGTTGCTGCGTCTATTTTTCCATGACTGCTTCATTGAGGGGTGTGATGCATCCGTTCTCTTGGATGACAGCAATGGGGATAAAAACCGTTCTATAGAGAAGCAGGCTACACCAAATAGGTCCTTAAAGGGATTTGATAAAATTGATCAGATCAAGGAGGAGCTCGAAAATGTTTGTCCAGGGGTGGTATCTTGTGCCGATATACTTGTTCTTGCCGCCAGAGATGGCATTATCCTGGCTGGTGGCCCATACTATCCAGTTTTGACAGGTAGAAGAGATAGTACTCAGTCCTACTTTGATGTGGCATTGGAGGAGATTCCAAAACCTGATGACAATATCACTCGTATATTGCATCTGTTCTCAAGTAGAGGTTTCAGTGAGAGAGAAACAGTTGCTCTTCTTGGAGCGCACAACATTGGAAAGATAGGATGTGAGTTCATACAGTCGCGCGTAAACAACTTCACTGGGACAGGGCAACCTGACCCAGCCTTACCTCTTGATCTCCTCAATACGATGAAACTAAAATGCCAAGACAATGCTAACAGGGCTACCAGTAATACTGATGCATCTCAATCGTCTTTGTCCATATCATCTGGGGCAGTTTTTGACTCTCACTATTACCAGAACTTGTTGCGGGGAAGAGGAATCCTCTTTGCTGATCAGCAATTAATGTCTAATAGGATGACAGCTCAAGTGGTCGCAGCATTTGCTTCAGATAATGGATCAGCATTTCGGCTGGACTTTTCCCAGGCAATGACGAAGATGTCAACACTTGGTGTCCTCACTGGATCCCAAGGTCAGTGTTGCAATGTTGAAAATGAAGAAGGGAAACTGTTTCCACATCTCAAGTCTTCTCTGAATGGTCAAAAGAGACTAGATGACCTTACAAGGCAAGTTGGAGTGTTTAATGGGTCAAGCAATTTAAAATATAAGACAGAATATGCAGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

439

Amino Acids

47.92

Weight (kDa)

5.88

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 107 - 361 1.8e-72 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1129
AccII CGCG 4 cut(s) 22, 35, 814, 816
AciI CCGC 2 cut(s) 582, 1000
AclWI GGATC 3 cut(s) 1096, 1149, 1162
AcoI YGGCCR 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 1062
AdeI CACNNNGTG 1 cut(s) 1142
AfaI GTAC 2 cut(s) 275, 649
AfiI CCNNNNNNNGG 1 cut(s) 318
AgsI TTSAA 1 cut(s) 1181
AjnI CCWGG 3 cut(s) 544, 600, 1113
AluBI AGCT 4 cut(s) 529, 1055, 1313, 1317
AluI AGCT 4 cut(s) 529, 1055, 1313, 1317
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 4 cut(s) 199, 302, 364, 1231
AlwI GGATC 3 cut(s) 1096, 1149, 1162
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 4 cut(s) 217, 343, 363, 610
ApeKI GCWGC 3 cut(s) 373, 1067, 1310
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AseI ATTAAT 1 cut(s) 1034
AspLEI GCGC 3 cut(s) 22, 775, 816
AspS9I GGNCC 3 cut(s) 364, 486, 611
AsuNHI GCTAGC 1 cut(s) 1313
AvaII GGWCC 1 cut(s) 486
AxyI CCTNAGG 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 11
BamHI GGATCC 1 cut(s) 1154
BanII GRGCYC 1 cut(s) 531
BarI GAAGNNNNNNTAC 2 cut(s) 631, 663
BauI CACGAG 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 1209
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 2 cut(s) 360, 1079
BccI CCATC 2 cut(s) 322, 584
BceAI ACGGC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 921, 955
BciT130I CCWGG 3 cut(s) 546, 602, 1115
BclI TGATCA 2 cut(s) 511, 1024
BcoDI GTCTC 4 cut(s) 199, 302, 364, 1231
BfaI CTAG 4 cut(s) 207, 1241, 1314, 1318
BfmI CTRYAG 1 cut(s) 459
BisI GCNGC 4 cut(s) 374, 582, 1068, 1311
BlsI GCNGC 4 cut(s) 375, 583, 1069, 1312
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 3 cut(s) 546, 602, 1115
Bme18I GGWCC 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 364, 486, 611
BmiI GGNNCC 1 cut(s) 1156
BmrFI CCNGG 3 cut(s) 546, 602, 1115
BmrI ACTGGG 2 cut(s) 361, 840
BmsI GCATC 5 cut(s) 403, 425, 727, 919, 941
BmtI GCTAGC 1 cut(s) 1317
BmuI ACTGGG 2 cut(s) 361, 840
BpiI GAAGAC 1 cut(s) 1209
BplI GAGNNNNNCTC 2 cut(s) 999, 1031
BpmI CTGGAG 1 cut(s) 290
BpuEI CTTGAG 3 cut(s) 712, 1041, 1196
BsaBI GATNNNNATC 1 cut(s) 282
BsaI GGTCTC 2 cut(s) 199, 302
BsaJI CCNNGG 3 cut(s) 545, 1113, 1159
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 6 cut(s) 186, 367, 623, 835, 918, 1156
Bse21I CCTNAGG 1 cut(s) 300
Bse3DI GCAATG 3 cut(s) 445, 1125, 1180
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 3 cut(s) 546, 602, 1115
BseDI CCNNGG 3 cut(s) 545, 1113, 1159
BseGI GGATG 4 cut(s) 416, 436, 800, 1053
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMI GCAATG 3 cut(s) 445, 1125, 1180
BseMII CTCAG 2 cut(s) 314, 665
BseNI ACTGG 6 cut(s) 186, 367, 623, 835, 918, 1156
BseRI GAGGAG 3 cut(s) 539, 692, 860
BseSI GKGCMC 1 cut(s) 11
BseXI GCAGC 2 cut(s) 360, 1079
BseYI CCCAGC 1 cut(s) 850
Bsh1236I CGCG 4 cut(s) 22, 35, 814, 816
BshFI GGCC 4 cut(s) 219, 345, 365, 612
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 847
BslI CCNNNNNNNGG 1 cut(s) 318
BsmAI GTCTC 4 cut(s) 199, 302, 364, 1231
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 847
BsnI GGCC 4 cut(s) 219, 345, 365, 612
Bso31I GGTCTC 2 cut(s) 199, 302
Bsp1286I GDGCHC 2 cut(s) 11, 531
Bsp143I GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
Bsp68I TCGCGA 1 cut(s) 35
BspACI CCGC 2 cut(s) 582, 1000
BspANI GGCC 4 cut(s) 219, 345, 365, 612
BspCNI CTCAG 2 cut(s) 313, 664
BspFNI CGCG 4 cut(s) 22, 35, 814, 816
BspLI GGNNCC 1 cut(s) 1156
BspOI GCTAGC 1 cut(s) 1317
BspPI GGATC 3 cut(s) 1096, 1149, 1162
BspQI GCTCTTC 2 cut(s) 168, 768
BspTNI GGTCTC 2 cut(s) 199, 302
BsrDI GCAATG 3 cut(s) 445, 1125, 1180
BsrFI RCCGGY 1 cut(s) 148
BsrI ACTGG 6 cut(s) 186, 367, 623, 835, 918, 1156
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 3 cut(s) 545, 1113, 1159
BssMI GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
BssSI CACGAG 1 cut(s) 292
BssT1I CCWWGG 1 cut(s) 1159
Bst2BI CACGAG 1 cut(s) 292
Bst2UI CCWGG 3 cut(s) 546, 602, 1115
Bst4CI ACNGT 6 cut(s) 13, 40, 455, 757, 810, 1200
Bst6I CTCTTC 5 cut(s) 118, 168, 634, 768, 1000
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstC8I GCNNGC 4 cut(s) 150, 347, 471, 1315
BstDEI CTNAG 2 cut(s) 300, 651
BstF5I GGATG 4 cut(s) 416, 436, 800, 1053
BstFNI CGCG 4 cut(s) 22, 35, 814, 816
BstHHI GCGC 3 cut(s) 22, 775, 816
BstKTI GATC 6 cut(s) 514, 519, 868, 1027, 1091, 1157
BstMAI GTCTC 4 cut(s) 199, 302, 364, 1231
BstMBI GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
BstMWI GCNNNNNNNGC 3 cut(s) 216, 911, 1073
BstNI CCWGG 3 cut(s) 546, 602, 1115
BstSCI CCNGG 3 cut(s) 544, 600, 1113
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 11
BstUI CGCG 4 cut(s) 22, 35, 814, 816
BstV1I GCAGC 2 cut(s) 360, 1079
BstV2I GAAGAC 1 cut(s) 1209
BstX2I RGATCY 1 cut(s) 1154
BstYI RGATCY 1 cut(s) 1154
Bsu36I CCTNAGG 1 cut(s) 300
BsuRI GGCC 4 cut(s) 219, 345, 365, 612
BtsCI GGATG 4 cut(s) 416, 436, 800, 1053
BtsIMutI CAGTG 7 cut(s) 18, 45, 179, 748, 828, 1149, 1172
BtuMI TCGCGA 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 150, 347, 471, 1315
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 3 cut(s) 22, 775, 816
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 364, 486, 611
CseI GACGC 1 cut(s) 365
Csp6I GTAC 2 cut(s) 274, 648
CviAII CATG 1 cut(s) 389
CviQI GTAC 2 cut(s) 274, 648
DdeI CTNAG 2 cut(s) 300, 651
DpnI GATC 6 cut(s) 513, 518, 867, 1026, 1090, 1156
DpnII GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
DraI TTTAAA 1 cut(s) 1290
DraIII CACNNNGTG 1 cut(s) 1142
DrdI GACNNNNNNGTC 1 cut(s) 1129
DseDI GACNNNNNNGTC 1 cut(s) 1129
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 5 cut(s) 118, 168, 634, 768, 1000
EarI CTCTTC 5 cut(s) 118, 168, 634, 768, 1000
Ecl136II GAGCTC 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 1159
Eco24I GRGCYC 1 cut(s) 531
Eco31I GGTCTC 2 cut(s) 199, 302
Eco47I GGWCC 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 1062
Eco81I CCTNAGG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 529
EcoO109I RGGNCCY 1 cut(s) 486
EcoRII CCWGG 3 cut(s) 544, 600, 1113
EcoT14I CCWWGG 1 cut(s) 1159
EcoT22I ATGCAT 2 cut(s) 418, 934
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 1159
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 1 cut(s) 392
FaqI GGGAC 1 cut(s) 847
FatI CATG 1 cut(s) 388
FauI CCCGC 1 cut(s) 993
FbaI TGATCA 2 cut(s) 511, 1024
Fnu4HI GCNGC 4 cut(s) 374, 582, 1068, 1311
FokI GGATG 4 cut(s) 403, 443, 807, 1060
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 4 cut(s) 374, 582, 1068, 1311
FspBI CTAG 4 cut(s) 207, 1241, 1314, 1318
GlaI GCGC 3 cut(s) 21, 774, 815
GluI GCNGC 4 cut(s) 374, 582, 1068, 1311
GsaI CCCAGC 1 cut(s) 854
GsuI CTGGAG 1 cut(s) 290
HaeIII GGCC 4 cut(s) 219, 345, 365, 612
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 365
HhaI GCGC 3 cut(s) 22, 775, 816
Hin1II CATG 1 cut(s) 392
Hin6I GCGC 3 cut(s) 20, 773, 814
HinP1I GCGC 3 cut(s) 20, 773, 814
HincII GTYRAC 1 cut(s) 1134
HindII GTYRAC 1 cut(s) 1134
HinfI GANTC 5 cut(s) 265, 337, 682, 974, 1011
HpaII CCGG 1 cut(s) 149
Hpy166II GTNNAC 2 cut(s) 820, 1134
Hpy188I TCNGA 5 cut(s) 172, 288, 516, 1081, 1224
Hpy188III TCNNGA 3 cut(s) 34, 269, 863
Hpy8I GTNNAC 2 cut(s) 820, 1134
Hpy99I CGWCG 6 cut(s) 163, 166, 169, 215, 247, 250
HpyAV CCTTC 2 cut(s) 10, 1184
HpyCH4III ACNGT 6 cut(s) 13, 40, 455, 757, 810, 1200
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 5 cut(s) 416, 718, 932, 1173, 1310
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 911, 1073
HpyF3I CTNAG 2 cut(s) 300, 651
HpySE526I ACGT 1 cut(s) 357
Hsp92II CATG 1 cut(s) 392
HspAI GCGC 3 cut(s) 20, 773, 814
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Ksp22I TGATCA 2 cut(s) 511, 1024
Kzo9I GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
LguI GCTCTTC 2 cut(s) 168, 768
LmnI GCTCC 3 cut(s) 69, 526, 770
Lsp1109I GCAGC 2 cut(s) 360, 1079
LweI GCATC 5 cut(s) 403, 425, 727, 919, 941
MaeI CTAG 4 cut(s) 207, 1241, 1314, 1318
MaeII ACGT 1 cut(s) 357
MalI GATC 6 cut(s) 513, 518, 867, 1026, 1090, 1156
MboI GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
MflI RGATCY 1 cut(s) 1154
MhlI GDGCHC 2 cut(s) 11, 531
MluCI AATT 3 cut(s) 507, 1031, 1285
MlyI GAGTC 1 cut(s) 968
MmeI TCCRAC 1 cut(s) 1243
Mph1103I ATGCAT 2 cut(s) 418, 934
MreI CGCCGGCG 1 cut(s) 148
MroNI GCCGGC 1 cut(s) 148
MseI TTAA 4 cut(s) 491, 1034, 1272, 1289
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 3 cut(s) 546, 602, 1115
MvaI CCWGG 3 cut(s) 546, 602, 1115
MvnI CGCG 4 cut(s) 22, 35, 814, 816
MwoI GCNNNNNNNGC 3 cut(s) 216, 911, 1073
NaeI GCCGGC 1 cut(s) 150
NdeII GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
NgoMIV GCCGGC 1 cut(s) 148
NheI GCTAGC 1 cut(s) 1313
NlaIII CATG 1 cut(s) 392
NlaIV GGNNCC 1 cut(s) 1156
NmeAIII GCCGAG 1 cut(s) 341
NruI TCGCGA 1 cut(s) 35
NsiI ATGCAT 2 cut(s) 418, 934
PciSI GCTCTTC 2 cut(s) 168, 768
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 150
PfeI GAWTC 4 cut(s) 265, 337, 682, 1011
PkrI GCNGC 4 cut(s) 375, 583, 1069, 1312
PleI GAGTC 1 cut(s) 968
PpsI GAGTC 1 cut(s) 968
PpuMI RGGWCCY 1 cut(s) 486
PshBI ATTAAT 1 cut(s) 1034
Psp124BI GAGCTC 1 cut(s) 531
Psp5II RGGWCCY 1 cut(s) 486
Psp6I CCWGG 3 cut(s) 544, 600, 1113
PspFI CCCAGC 1 cut(s) 850
PspGI CCWGG 3 cut(s) 544, 600, 1113
PspN4I GGNNCC 1 cut(s) 1156
PspPI GGNCC 3 cut(s) 364, 486, 611
PspPPI RGGWCCY 1 cut(s) 486
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 1154
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 2 cut(s) 275, 649
RsaNI GTAC 2 cut(s) 274, 648
SacI GAGCTC 1 cut(s) 531
SapI GCTCTTC 2 cut(s) 168, 768
SaqAI TTAA 4 cut(s) 491, 1034, 1272, 1289
SatI GCNGC 4 cut(s) 374, 582, 1068, 1311
Sau3AI GATC 6 cut(s) 511, 516, 865, 1024, 1088, 1154
Sau96I GGNCC 3 cut(s) 364, 486, 611
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 1 cut(s) 968
ScrFI CCNGG 3 cut(s) 546, 602, 1115
SduI GDGCHC 2 cut(s) 11, 531
SfaNI GCATC 5 cut(s) 403, 425, 727, 919, 941
SfcI CTRYAG 1 cut(s) 459
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 1 cut(s) 486
SmlI CTYRAG 3 cut(s) 727, 1056, 1211
SmoI CTYRAG 3 cut(s) 727, 1056, 1211
Sse9I AATT 3 cut(s) 507, 1031, 1285
SsiI CCGC 2 cut(s) 582, 1000
SspMI CTAG 4 cut(s) 207, 1241, 1314, 1318
SstI GAGCTC 1 cut(s) 531
StyD4I CCNGG 3 cut(s) 544, 600, 1113
StyI CCWWGG 1 cut(s) 1159
TaaI ACNGT 6 cut(s) 13, 40, 455, 757, 810, 1200
TaiI ACGT 1 cut(s) 360
TaqI TCGA 2 cut(s) 323, 531
TasI AATT 3 cut(s) 507, 1031, 1285
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 4 cut(s) 265, 337, 682, 1011
Tru1I TTAA 4 cut(s) 491, 1034, 1272, 1289
Tru9I TTAA 4 cut(s) 491, 1034, 1272, 1289
TscAI CASTG 7 cut(s) 18, 45, 186, 748, 835, 1156, 1172
TseI GCWGC 3 cut(s) 373, 1067, 1310
TspDTI ATGAA 4 cut(s) 388, 793, 896, 1200
TspGWI ACGGA 3 cut(s) 228, 263, 409
TspRI CASTG 7 cut(s) 18, 45, 186, 748, 835, 1156, 1172
VpaK11BI GGWCC 1 cut(s) 486
VspI ATTAAT 1 cut(s) 1034
XspI CTAG 4 cut(s) 207, 1241, 1314, 1318
ZrmI AGTACT 1 cut(s) 649
Zsp2I ATGCAT 2 cut(s) 418, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.