Rroxscaffold_1G00054280

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001171
Physical Location & Seq
Reverse (-)
75655649 .. 75657683
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054280.1

Sequence Viewer

Length: 465 bp
ATGGCTTCTAGCGACAGAGGCCAAGTAGAGTTCTTCACCGAAAGGACGACGGATGAGAGGTTGGGGAATCTGGAGTACGATTTCTATCGGAGCTCGTGTCCTCAGGCAGAGACCATTGTGAGGTCGAAGATGGCTTGGATTTATGGCCAGCACAGGAACGTCTCGGCCCAGTTGCTGCGTCTATTTTTCCATGACTGCTCATTGAGAGCTTTCTATTGTATTAAGCTTCTTCTGATTTTTACTGGTGACTATCAAGATGGGTGTGATGCATCCGTTCTCTTGGATGACAGCAATGGGGATAAAAACCATTCTATAGAGAACCAGGCTACACCAAAGAGGTCCTTGAAGGGATTCGATAAAATTGATCAGATCAAGGAGGAACTCGAAAATGTTTGTCCAGGGGTGGTATCTTGTGCTGATGTACTTGCTCTTGCCGCCAGAGATGGCATTATTCTGGTGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

154

Amino Acids

17.37

Weight (kDa)

5.02

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 40 - 151 1.3e-25 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 435
AcoI YGGCCR 1 cut(s) 145
AfaI GTAC 2 cut(s) 77, 423
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 2 cut(s) 321, 397
AluBI AGCT 3 cut(s) 93, 209, 226
AluI AGCT 3 cut(s) 93, 209, 226
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 2 cut(s) 104, 166
AlwNI CAGNNNCTG 1 cut(s) 175
AoxI GGCC 3 cut(s) 19, 145, 165
ApeKI GCWGC 1 cut(s) 175
Asp700I GAANNNNTTC 1 cut(s) 350
AspS9I GGNCC 2 cut(s) 166, 339
AsuHPI GGTGA 2 cut(s) 28, 257
AvaII GGWCC 1 cut(s) 339
AxyI CCTNAGG 1 cut(s) 102
BalI TGGCCA 1 cut(s) 147
BanII GRGCYC 1 cut(s) 95
BauI CACGAG 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 162
BccI CCATC 3 cut(s) 124, 251, 437
BciT130I CCWGG 2 cut(s) 323, 399
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 104, 166
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 2 cut(s) 176, 435
BlsI GCNGC 2 cut(s) 177, 436
Bme1390I CCNGG 2 cut(s) 323, 399
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 166, 339
BmrFI CCNGG 2 cut(s) 323, 399
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 256, 278
BmuI ACTGGG 1 cut(s) 163
BpmI CTGGAG 1 cut(s) 92
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 398
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 169, 247
Bse21I CCTNAGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 298
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 2 cut(s) 323, 399
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 3 cut(s) 58, 269, 289
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 2 cut(s) 169, 247
BseXI GCAGC 1 cut(s) 162
BshFI GGCC 3 cut(s) 21, 147, 167
BsiHKAI GWGCWC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 2 cut(s) 104, 166
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 3 cut(s) 21, 147, 167
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 2 cut(s) 364, 369
BspACI CCGC 1 cut(s) 435
BspANI GGCC 3 cut(s) 21, 147, 167
BspCNI CTCAG 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 298
BsrI ACTGG 2 cut(s) 169, 247
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 2 cut(s) 364, 369
BssSI CACGAG 1 cut(s) 94
Bst2BI CACGAG 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 323, 399
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 58, 269, 289
BstKTI GATC 2 cut(s) 367, 372
BstMAI GTCTC 2 cut(s) 104, 166
BstMBI GATC 2 cut(s) 364, 369
BstMWI GCNNNNNNNGC 2 cut(s) 18, 434
BstNI CCWGG 2 cut(s) 323, 399
BstSCI CCNGG 2 cut(s) 321, 397
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 102
BsuRI GGCC 3 cut(s) 21, 147, 167
BtsCI GGATG 3 cut(s) 58, 269, 289
Cac8I GCNNGC 1 cut(s) 149
CaiI CAGNNNCTG 1 cut(s) 175
Cfr13I GGNCC 2 cut(s) 166, 339
CseI GACGC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 76, 422
CviAII CATG 1 cut(s) 191
CviJI RGCY 9 cut(s) 5, 21, 93, 134, 147, 167, 209, 226, 326
CviKI_1 RGCY 9 cut(s) 5, 21, 93, 134, 147, 167, 209, 226, 326
CviQI GTAC 2 cut(s) 76, 422
DdeI CTNAG 1 cut(s) 102
DpnI GATC 2 cut(s) 366, 371
DpnII GATC 2 cut(s) 364, 369
EaeI YGGCCR 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 95
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 93
Eco81I CCTNAGG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 93
EcoO109I RGGNCCY 1 cut(s) 339
EcoRII CCWGG 2 cut(s) 321, 397
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 95
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 1 cut(s) 194
FaiI YATR 3 cut(s) 144, 192, 314
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FatI CATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 176, 435
FokI GGATG 3 cut(s) 65, 256, 296
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 2 cut(s) 176, 435
FspBI CTAG 1 cut(s) 9
GluI GCNGC 2 cut(s) 176, 435
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 3 cut(s) 21, 147, 167
HgaI GACGC 1 cut(s) 167
Hin1II CATG 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 2 cut(s) 67, 351
HphI GGTGA 2 cut(s) 28, 257
Hpy188I TCNGA 3 cut(s) 90, 234, 369
Hpy188III TCNNGA 2 cut(s) 71, 254
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 434
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 2 cut(s) 364, 369
LmnI GCTCC 1 cut(s) 90
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 2 cut(s) 256, 278
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 245
MalI GATC 2 cut(s) 366, 371
MboI GATC 2 cut(s) 364, 369
MboII GAAGA 3 cut(s) 25, 139, 221
MhlI GDGCHC 1 cut(s) 95
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 1 cut(s) 360
MluNI TGGCCA 1 cut(s) 147
MnlI CCTC 6 cut(s) 11, 51, 111, 114, 330, 370
Mox20I TGGCCA 1 cut(s) 147
Mph1103I ATGCAT 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 350
MscI TGGCCA 1 cut(s) 147
MseI TTAA 1 cut(s) 222
Msp20I TGGCCA 1 cut(s) 147
MspR9I CCNGG 2 cut(s) 323, 399
MvaI CCWGG 2 cut(s) 323, 399
MwoI GCNNNNNNNGC 2 cut(s) 18, 434
NdeII GATC 2 cut(s) 364, 369
NlaIII CATG 1 cut(s) 194
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 245
NsiI ATGCAT 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 2 cut(s) 67, 351
PkrI GCNGC 2 cut(s) 177, 436
PpuMI RGGWCCY 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 321, 397
PspGI CCWGG 2 cut(s) 321, 397
PspPI GGNCC 2 cut(s) 166, 339
PspPPI RGGWCCY 1 cut(s) 339
PstNI CAGNNNCTG 1 cut(s) 175
RsaI GTAC 2 cut(s) 77, 423
RsaNI GTAC 2 cut(s) 76, 422
SacI GAGCTC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 2 cut(s) 176, 435
Sau3AI GATC 2 cut(s) 364, 369
Sau96I GGNCC 2 cut(s) 166, 339
ScrFI CCNGG 2 cut(s) 323, 399
SduI GDGCHC 1 cut(s) 95
SetI ASST 7 cut(s) 62, 95, 125, 162, 211, 228, 341
SfaNI GCATC 2 cut(s) 256, 278
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 339
Sse9I AATT 1 cut(s) 360
SsiI CCGC 1 cut(s) 435
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 321, 397
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 125, 354, 384
TasI AATT 1 cut(s) 360
TatI WGTACW 1 cut(s) 421
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 2 cut(s) 67, 351
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 245
TspGWI ACGGA 2 cut(s) 65, 262
VpaK11BI GGWCC 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 1 cut(s) 9
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.