Rh5BG178600

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001171
Physical Location & Seq
Reverse (-)
19365942 .. 19368906
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG178600.1

Sequence Viewer

Length: 1299 bp
ATGACTAACTTCTACGTCCTTTCTGATTCTCATTTCCCGCTTCAACGTGACCCTCAAAACGACGCCGTTTGCGAGCTCGACACCAGCGAAAGGAAGAAGAAGAAGAAGATGTTGGGCACAGTGAAGGCGCGTTGGGTGTTCGCGACGGTGGTGTTTTTCTCGGTGGTGGTTTCGCGGAGCAAACCGAGAGTTGAGTCGGAGAGAGAGAAGCCCTTTCTCTCTTCTTCTTCTTCTTCTTCGCCGGCGAAGTTGTCGTCGGCGTCCGAAGAGCCACTGGTCACTCTGGTCTCTATGGCTTCTACCGACGGAGGCCAAGCAGAGTTCTACACCGAAACGACGACGGATGAGGGGTCGCGGAATCTGGAGTACGATTTCTATCGGAACTCGTGCCCTCAGGCAGAGACCATTGTGAGGTCGAAGATGGCTTGGATTTACGGCCAGCACAGGAACGTCTCGGCCCAGTTGCTGCGTCTCTTTTTCCATGACTGCTTCATTGAGGGGTGTGATGCATCCGTTCTCTTGGATGACAGCAATGGGGATAAAAACCGTTCTATAGAGAAGCAGGCCACACCAAATAGGTCCTTGAAGGGATTTGATAAAATTGATCAGATCAAGGAGGAGCTCGAAAATGTTTGTCCAGGGGTGGTATCTTGTGCCGATATACTTGTTCTTGCCGCCAGAGATGGCATTATCCTGGCTGGTGGCCCATATTATCCAGTCTTGACAGGTAGAAGAGATAGTACCCAGTCCTACTTTGATGTGGCATTGGAGGAGATTCCAAAACCTGATGACAATATCACTCATATATTGCATCTGTTCTCAAGTAGAGGTTTCAGTGAGAGAGAAACAGTTGCTCTTCTTGGAGCGCACAACATTGGAAAGATAGGATGTGAGTTCATACAGTCACGGGTAAACAACTTCACTGGGACAGGGCAACCAGACCCAGCCTTACCTCTTGATCTCCTCACTACGATGAAACTAAAATGCCAAGACAATGCTAACAGGGCGACCAGTAATACTGATGCATCTCAATCGTCTTTGTCCATATCATCTGGGGCAGTTTTTGACTCTCACTATTACCAGAACTTGTTGCGGGGAAGAGGAATCCTCTTTGCTGATCAGCAATTAATGGCTAATAGGATGACAGCTCAAGTGGTCGCAGCATTTGCTTCGGATAATGGATCGGCATTTCGAATGGACTTTGCCCAGGCAATGACGAAGATGTCAACACTTGGTGTCCTCACTGGATCTCAAGGTCAGGTGCGATCGGTCTGTTCTCTCCCAGTCAATAGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

432

Amino Acids

47.57

Weight (kDa)

5.88

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 137 - 392 5.7e-73 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1222
AccII CGCG 4 cut(s) 130, 143, 175, 355
AciI CCGC 5 cut(s) 38, 175, 355, 675, 1093
AclWI GGATC 2 cut(s) 1189, 1255
AcoI YGGCCR 1 cut(s) 436
AcyI GRCGYC 2 cut(s) 63, 260
AdeI CACNNNGTG 1 cut(s) 1235
AfaI GTAC 2 cut(s) 368, 742
AfiI CCNNNNNNNGG 2 cut(s) 90, 411
AgsI TTSAA 2 cut(s) 44, 586
AjnI CCWGG 3 cut(s) 637, 693, 1206
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 3 cut(s) 76, 622, 1148
AluI AGCT 3 cut(s) 76, 622, 1148
Alw21I GWGCWC 2 cut(s) 78, 624
Alw26I GTCTC 4 cut(s) 292, 395, 457, 476
AlwI GGATC 2 cut(s) 1189, 1255
AlwNI CAGNNNCTG 1 cut(s) 466
AoxI GGCC 5 cut(s) 310, 436, 456, 564, 703
ApeKI GCWGC 2 cut(s) 466, 1160
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AseI ATTAAT 1 cut(s) 1127
AspLEI GCGC 2 cut(s) 130, 868
AspS9I GGNCC 3 cut(s) 457, 579, 704
AsuII TTCGAA 1 cut(s) 1192
AvaII GGWCC 1 cut(s) 579
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 2 cut(s) 119, 392
BanII GRGCYC 2 cut(s) 78, 624
BarI GAAGNNNNNNTAC 2 cut(s) 724, 756
BauI CACGAG 1 cut(s) 385
Bbv12I GWGCWC 2 cut(s) 78, 624
BbvI GCAGC 2 cut(s) 453, 1172
BccI CCATC 2 cut(s) 415, 677
BceAI ACGGC 2 cut(s) 50, 451
BcgI CGANNNNNNTGC 4 cut(s) 1014, 1048, 1152, 1186
BciT130I CCWGG 3 cut(s) 639, 695, 1208
BclI TGATCA 2 cut(s) 604, 1117
BcoDI GTCTC 4 cut(s) 292, 395, 457, 476
BfmI CTRYAG 1 cut(s) 552
BisI GCNGC 3 cut(s) 467, 675, 1161
BlsI GCNGC 3 cut(s) 468, 676, 1162
Bme1390I CCNGG 3 cut(s) 639, 695, 1208
Bme18I GGWCC 1 cut(s) 579
BmgT120I GGNCC 3 cut(s) 457, 579, 704
BmrFI CCNGG 3 cut(s) 639, 695, 1208
BmrI ACTGGG 4 cut(s) 454, 739, 933, 1277
BmsI GCATC 5 cut(s) 496, 518, 820, 1012, 1034
BmuI ACTGGG 4 cut(s) 454, 739, 933, 1277
BplI GAGNNNNNCTC 2 cut(s) 1092, 1124
BpmI CTGGAG 1 cut(s) 383
Bpu14I TTCGAA 1 cut(s) 1192
BpuEI CTTGAG 3 cut(s) 805, 1134, 1236
BsaBI GATNNNNATC 1 cut(s) 375
BsaHI GRCGYC 2 cut(s) 63, 260
BsaI GGTCTC 2 cut(s) 292, 395
BsaJI CCNNGG 2 cut(s) 638, 1206
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 411
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 8 cut(s) 279, 460, 716, 745, 928, 1011, 1249, 1283
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 2 cut(s) 538, 1218
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 3 cut(s) 639, 695, 1208
BseDI CCNNGG 2 cut(s) 638, 1206
BseGI GGATG 5 cut(s) 349, 509, 529, 893, 1146
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 90, 411
BseMI GCAATG 2 cut(s) 538, 1218
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 8 cut(s) 279, 460, 716, 745, 928, 1011, 1249, 1283
BseRI GAGGAG 3 cut(s) 632, 785, 953
BseSI GKGCMC 2 cut(s) 119, 392
BseXI GCAGC 2 cut(s) 453, 1172
BseYI CCCAGC 1 cut(s) 943
Bsh1236I CGCG 4 cut(s) 130, 143, 175, 355
Bsh1285I CGRYCG 1 cut(s) 1268
BshFI GGCC 5 cut(s) 312, 438, 458, 566, 705
BsiEI CGRYCG 1 cut(s) 1268
BsiHKAI GWGCWC 2 cut(s) 78, 624
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 2 cut(s) 90, 411
BsmAI GTCTC 4 cut(s) 292, 395, 457, 476
BsmBI CGTCTC 2 cut(s) 457, 476
BsmFI GGGAC 1 cut(s) 940
BsnI GGCC 5 cut(s) 312, 438, 458, 566, 705
Bso31I GGTCTC 2 cut(s) 292, 395
Bsp119I TTCGAA 1 cut(s) 1192
Bsp1286I GDGCHC 4 cut(s) 78, 119, 392, 624
Bsp143I GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
Bsp68I TCGCGA 1 cut(s) 143
BspACI CCGC 5 cut(s) 38, 175, 355, 675, 1093
BspANI GGCC 5 cut(s) 312, 438, 458, 566, 705
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 4 cut(s) 130, 143, 175, 355
BspPI GGATC 2 cut(s) 1189, 1255
BspQI GCTCTTC 2 cut(s) 261, 861
BspT104I TTCGAA 1 cut(s) 1192
BspTNI GGTCTC 2 cut(s) 292, 395
BsrDI GCAATG 2 cut(s) 538, 1218
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 8 cut(s) 279, 460, 716, 745, 928, 1011, 1249, 1283
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 638, 1206
BssMI GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
BssNI GRCGYC 2 cut(s) 63, 260
BssSI CACGAG 1 cut(s) 385
Bst2BI CACGAG 1 cut(s) 385
Bst2UI CCWGG 3 cut(s) 639, 695, 1208
Bst4CI ACNGT 5 cut(s) 121, 148, 548, 850, 903
Bst6I CTCTTC 5 cut(s) 226, 261, 727, 861, 1093
BstACI GRCGYC 2 cut(s) 63, 260
BstAPI GCANNNNNTGC 1 cut(s) 1166
BstBI TTCGAA 1 cut(s) 1192
BstC8I GCNNGC 4 cut(s) 74, 243, 440, 564
BstDEI CTNAG 1 cut(s) 393
BstF5I GGATG 5 cut(s) 349, 509, 529, 893, 1146
BstFNI CGCG 4 cut(s) 130, 143, 175, 355
BstHHI GCGC 2 cut(s) 130, 868
BstKTI GATC 7 cut(s) 607, 612, 961, 1120, 1184, 1250, 1268
BstMAI GTCTC 4 cut(s) 292, 395, 457, 476
BstMBI GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
BstMCI CGRYCG 1 cut(s) 1268
BstMWI GCNNNNNNNGC 2 cut(s) 1004, 1166
BstNI CCWGG 3 cut(s) 639, 695, 1208
BstSCI CCNGG 3 cut(s) 637, 693, 1206
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 2 cut(s) 119, 392
BstUI CGCG 4 cut(s) 130, 143, 175, 355
BstV1I GCAGC 2 cut(s) 453, 1172
BstX2I RGATCY 1 cut(s) 1247
BstYI RGATCY 1 cut(s) 1247
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 5 cut(s) 312, 438, 458, 566, 705
BtsCI GGATG 5 cut(s) 349, 509, 529, 893, 1146
BtsIMutI CAGTG 5 cut(s) 126, 272, 841, 921, 1242
BtuMI TCGCGA 1 cut(s) 143
Cac8I GCNNGC 4 cut(s) 74, 243, 440, 564
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 2 cut(s) 130, 868
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 3 cut(s) 457, 579, 704
CseI GACGC 3 cut(s) 71, 249, 458
Csp6I GTAC 2 cut(s) 367, 741
CviAII CATG 1 cut(s) 482
CviQI GTAC 2 cut(s) 367, 741
DdeI CTNAG 1 cut(s) 393
DpnI GATC 7 cut(s) 606, 611, 960, 1119, 1183, 1249, 1267
DpnII GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
DraIII CACNNNGTG 1 cut(s) 1235
DrdI GACNNNNNNGTC 1 cut(s) 1222
DseDI GACNNNNNNGTC 1 cut(s) 1222
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 5 cut(s) 226, 261, 727, 861, 1093
EarI CTCTTC 5 cut(s) 226, 261, 727, 861, 1093
Ecl136II GAGCTC 2 cut(s) 76, 622
Eco24I GRGCYC 2 cut(s) 78, 624
Eco31I GGTCTC 2 cut(s) 292, 395
Eco47I GGWCC 1 cut(s) 579
Eco53kI GAGCTC 2 cut(s) 76, 622
Eco81I CCTNAGG 1 cut(s) 393
EcoICRI GAGCTC 2 cut(s) 76, 622
EcoO109I RGGNCCY 1 cut(s) 579
EcoRII CCWGG 3 cut(s) 637, 693, 1206
EcoT22I ATGCAT 2 cut(s) 511, 1027
EcoT38I GRGCYC 2 cut(s) 78, 624
Esp3I CGTCTC 2 cut(s) 457, 476
FaeI CATG 1 cut(s) 485
FaiI YATR 9 cut(s) 293, 483, 554, 662, 709, 804, 806, 899, 1046
FaqI GGGAC 1 cut(s) 940
FatI CATG 1 cut(s) 481
FauI CCCGC 2 cut(s) 45, 1086
FbaI TGATCA 2 cut(s) 604, 1117
Fnu4HI GCNGC 3 cut(s) 467, 675, 1161
FokI GGATG 5 cut(s) 356, 496, 536, 900, 1153
FriOI GRGCYC 2 cut(s) 78, 624
Fsp4HI GCNGC 3 cut(s) 467, 675, 1161
GlaI GCGC 2 cut(s) 129, 867
GluI GCNGC 3 cut(s) 467, 675, 1161
GsaI CCCAGC 1 cut(s) 947
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 5 cut(s) 312, 438, 458, 566, 705
HapII CCGG 1 cut(s) 242
HgaI GACGC 3 cut(s) 71, 249, 458
HhaI GCGC 2 cut(s) 130, 868
Hin1I GRCGYC 2 cut(s) 63, 260
Hin1II CATG 1 cut(s) 485
Hin6I GCGC 2 cut(s) 128, 866
HinP1I GCGC 2 cut(s) 128, 866
HincII GTYRAC 1 cut(s) 1227
HindII GTYRAC 1 cut(s) 1227
HinfI GANTC 6 cut(s) 26, 194, 358, 775, 1067, 1104
HpaII CCGG 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 913, 1227
Hpy188I TCNGA 6 cut(s) 25, 199, 265, 381, 609, 1174
Hpy188III TCNNGA 4 cut(s) 142, 362, 721, 956
Hpy8I GTNNAC 2 cut(s) 913, 1227
Hpy99I CGWCG 6 cut(s) 65, 148, 259, 308, 340, 343
HpyAV CCTTC 2 cut(s) 118, 580
HpyCH4III ACNGT 5 cut(s) 121, 148, 548, 850, 903
HpyCH4IV ACGT 3 cut(s) 15, 46, 450
HpyCH4V TGCA 3 cut(s) 509, 811, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 1004, 1166
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 3 cut(s) 15, 46, 450
Hsp92I GRCGYC 2 cut(s) 63, 260
Hsp92II CATG 1 cut(s) 485
HspAI GCGC 2 cut(s) 128, 866
KroI GCCGGC 1 cut(s) 241
KroNI GCCGGC 1 cut(s) 243
Ksp22I TGATCA 2 cut(s) 604, 1117
Kzo9I GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
LguI GCTCTTC 2 cut(s) 261, 861
LmnI GCTCC 3 cut(s) 177, 619, 863
Lsp1109I GCAGC 2 cut(s) 453, 1172
LweI GCATC 5 cut(s) 496, 518, 820, 1012, 1034
MaeII ACGT 3 cut(s) 15, 46, 450
MaeIII GTNAC 3 cut(s) 47, 277, 903
MalI GATC 7 cut(s) 606, 611, 960, 1119, 1183, 1249, 1267
MboI GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
MflI RGATCY 1 cut(s) 1247
MhlI GDGCHC 4 cut(s) 78, 119, 392, 624
MluCI AATT 2 cut(s) 600, 1124
MlyI GAGTC 2 cut(s) 203, 1061
MmeI TCCRAC 1 cut(s) 177
Mph1103I ATGCAT 2 cut(s) 511, 1027
MreI CGCCGGCG 1 cut(s) 241
MroNI GCCGGC 1 cut(s) 241
MseI TTAA 1 cut(s) 1127
MslI CAYNNNNRTG 1 cut(s) 971
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 3 cut(s) 639, 695, 1208
MvaI CCWGG 3 cut(s) 639, 695, 1208
MvnI CGCG 4 cut(s) 130, 143, 175, 355
MwoI GCNNNNNNNGC 2 cut(s) 1004, 1166
NaeI GCCGGC 1 cut(s) 243
NdeII GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
NgoMIV GCCGGC 1 cut(s) 241
NlaIII CATG 1 cut(s) 485
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 3 cut(s) 47, 277, 903
NruI TCGCGA 1 cut(s) 143
NsiI ATGCAT 2 cut(s) 511, 1027
NspV TTCGAA 1 cut(s) 1192
PciSI GCTCTTC 2 cut(s) 261, 861
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 84
PdiI GCCGGC 1 cut(s) 243
PfeI GAWTC 4 cut(s) 26, 358, 775, 1104
PkrI GCNGC 3 cut(s) 468, 676, 1162
Ple19I CGATCG 1 cut(s) 1268
PleI GAGTC 2 cut(s) 202, 1061
PpsI GAGTC 2 cut(s) 202, 1061
PpuMI RGGWCCY 1 cut(s) 579
PshBI ATTAAT 1 cut(s) 1127
Psp124BI GAGCTC 2 cut(s) 78, 624
Psp5II RGGWCCY 1 cut(s) 579
Psp6I CCWGG 3 cut(s) 637, 693, 1206
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 3 cut(s) 637, 693, 1206
PspPI GGNCC 3 cut(s) 457, 579, 704
PspPPI RGGWCCY 1 cut(s) 579
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 1247
PvuI CGATCG 1 cut(s) 1268
RruI TCGCGA 1 cut(s) 143
RsaI GTAC 2 cut(s) 368, 742
RsaNI GTAC 2 cut(s) 367, 741
RseI CAYNNNNRTG 1 cut(s) 971
SacI GAGCTC 2 cut(s) 78, 624
SapI GCTCTTC 2 cut(s) 261, 861
SaqAI TTAA 1 cut(s) 1127
SatI GCNGC 3 cut(s) 467, 675, 1161
Sau3AI GATC 7 cut(s) 604, 609, 958, 1117, 1181, 1247, 1265
Sau96I GGNCC 3 cut(s) 457, 579, 704
SchI GAGTC 2 cut(s) 203, 1061
ScrFI CCNGG 3 cut(s) 639, 695, 1208
SduI GDGCHC 4 cut(s) 78, 119, 392, 624
SfaNI GCATC 5 cut(s) 496, 518, 820, 1012, 1034
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 1192
SgrAI CRCCGGYG 1 cut(s) 241
SinI GGWCC 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 971
SmlI CTYRAG 3 cut(s) 820, 1149, 1251
SmoI CTYRAG 3 cut(s) 820, 1149, 1251
Sse9I AATT 2 cut(s) 600, 1124
SsiI CCGC 5 cut(s) 38, 175, 355, 675, 1093
SstI GAGCTC 2 cut(s) 78, 624
StyD4I CCNGG 3 cut(s) 637, 693, 1206
TaaI ACNGT 5 cut(s) 121, 148, 548, 850, 903
TaiI ACGT 3 cut(s) 18, 49, 453
TaqI TCGA 4 cut(s) 78, 416, 624, 1192
TaqII GACCGA 1 cut(s) 1258
TasI AATT 2 cut(s) 600, 1124
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 4 cut(s) 26, 358, 775, 1104
Tru1I TTAA 1 cut(s) 1127
Tru9I TTAA 1 cut(s) 1127
TscAI CASTG 5 cut(s) 126, 279, 841, 928, 1249
TseFI GTSAC 3 cut(s) 47, 277, 903
TseI GCWGC 2 cut(s) 466, 1160
Tsp45I GTSAC 3 cut(s) 47, 277, 903
TspDTI ATGAA 3 cut(s) 481, 886, 989
TspGWI ACGGA 3 cut(s) 321, 356, 502
TspRI CASTG 5 cut(s) 126, 279, 841, 928, 1249
VpaK11BI GGWCC 1 cut(s) 579
VspI ATTAAT 1 cut(s) 1127
Zsp2I ATGCAT 2 cut(s) 511, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.