Rroxscaffold_3G00223150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002159
Physical Location & Seq
Forward (+)
6281313 .. 6300617
19305 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00223150.1

Sequence Viewer

Length: 459 bp
ATGGCGGCACAGCTTTTGCTTCTCGGCAGTTGCGAAGAACTCTTCAATCCTATCGATCGAGGGACAAAGAACGTCTTCTTTTGGTTCGAGAACCTCAAATCTCGAGAAAGCCGGCAGATGAAGGCAATTCGGAACGTTCGGGTTGCGGAGCTTGTTTTCAAAATCGCGAAGCTAGCTTTCTCTCCTCTCGTCTTCTCTACAAATCACGGAGGTTGGGCTTCAATTAGTGTTGATATCATAAAAGATTATAAAAAAGACAAAAAATTAAGAAAGAGAGATGATGAAGGACAAACTTCTTCGTACCCGAAGCTGGCGAATCAGAAGAAGCCCTTGATCTCAGACCAATTTAGACCCGATCCTTCAATGTCCAGTACTACGCGGCCGTGGAAAACTGAAGTTATCTCGAAGAAAGTAGTCATTGCACTTGGCAAGAGTGTAGTTGGAAGTATCAAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.25

Weight (kDa)

9.96

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AccII CGCG 2 cut(s) 167, 379
AciI CCGC 3 cut(s) 5, 146, 379
AclI AACGTT 1 cut(s) 135
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 380
AcuI CTGAAG 1 cut(s) 414
AfaI GTAC 2 cut(s) 302, 373
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 4 cut(s) 46, 160, 222, 363
AluBI AGCT 5 cut(s) 13, 151, 172, 176, 310
AluI AGCT 5 cut(s) 13, 151, 172, 176, 310
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 74
AsuNHI GCTAGC 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 102
BbsI GAAGAC 2 cut(s) 67, 184
BceAI ACGGC 1 cut(s) 367
BfaI CTAG 1 cut(s) 173
BisI GCNGC 2 cut(s) 6, 380
BlsI GCNGC 2 cut(s) 7, 381
BmcAI AGTACT 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 102
BmtI GCTAGC 1 cut(s) 176
BpiI GAAGAC 2 cut(s) 67, 184
Bsa29I ATCGAT 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 383
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 369
Bse3DI GCAATG 1 cut(s) 417
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 369
BseRI GAGGAG 1 cut(s) 174
BseX3I CGGCCG 1 cut(s) 380
Bsh1236I CGCG 2 cut(s) 167, 379
Bsh1285I CGRYCG 2 cut(s) 58, 383
BshFI GGCC 1 cut(s) 382
BshVI ATCGAT 1 cut(s) 54
BsiEI CGRYCG 2 cut(s) 58, 383
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 1 cut(s) 112
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 1 cut(s) 382
BsoBI CYCGRG 1 cut(s) 102
Bsp143I GATC 3 cut(s) 55, 333, 355
Bsp68I TCGCGA 1 cut(s) 167
BspACI CCGC 3 cut(s) 5, 146, 379
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 1 cut(s) 350
BspDI ATCGAT 1 cut(s) 54
BspFNI CGCG 2 cut(s) 167, 379
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 111
BsrI ACTGG 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 3 cut(s) 55, 333, 355
Bst6I CTCTTC 1 cut(s) 47
BstC8I GCNNGC 3 cut(s) 113, 174, 312
BstDEI CTNAG 1 cut(s) 337
BstDSI CCRYGG 1 cut(s) 383
BstFNI CGCG 2 cut(s) 167, 379
BstKTI GATC 3 cut(s) 58, 336, 358
BstMBI GATC 3 cut(s) 55, 333, 355
BstMCI CGRYCG 2 cut(s) 58, 383
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstUI CGCG 2 cut(s) 167, 379
BstV2I GAAGAC 2 cut(s) 67, 184
BstZI CGGCCG 1 cut(s) 380
Bsu15I ATCGAT 1 cut(s) 54
BsuRI GGCC 1 cut(s) 382
BsuTUI ATCGAT 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 383
BtuMI TCGCGA 1 cut(s) 167
Cac8I GCNNGC 3 cut(s) 113, 174, 312
Cfr10I RCCGGY 1 cut(s) 111
ClaI ATCGAT 1 cut(s) 54
Csp6I GTAC 2 cut(s) 301, 372
CviJI RGCY 9 cut(s) 13, 111, 151, 172, 176, 218, 310, 328, 382
CviKI_1 RGCY 9 cut(s) 13, 111, 151, 172, 176, 218, 310, 328, 382
CviQI GTAC 2 cut(s) 301, 372
DdeI CTNAG 1 cut(s) 337
DpnI GATC 3 cut(s) 57, 335, 357
DpnII GATC 3 cut(s) 55, 333, 355
EaeI YGGCCR 1 cut(s) 380
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EclXI CGGCCG 1 cut(s) 380
Eco32I GATATC 1 cut(s) 235
Eco52I CGGCCG 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 102
EcoRV GATATC 1 cut(s) 235
FaiI YATR 2 cut(s) 239, 249
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 314, 346
FaqI GGGAC 1 cut(s) 76
Fnu4HI GCNGC 2 cut(s) 6, 380
Fsp4HI GCNGC 2 cut(s) 6, 380
FspBI CTAG 1 cut(s) 173
GluI GCNGC 2 cut(s) 6, 380
HaeIII GGCC 1 cut(s) 382
HapII CCGG 1 cut(s) 112
HinfI GANTC 1 cut(s) 316
HpaII CCGG 1 cut(s) 112
Hpy188I TCNGA 3 cut(s) 132, 321, 340
Hpy188III TCNNGA 5 cut(s) 88, 102, 104, 166, 403
HpyAV CCTTC 3 cut(s) 115, 278, 369
HpyCH4IV ACGT 2 cut(s) 72, 135
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 1 cut(s) 337
HpySE526I ACGT 2 cut(s) 72, 135
KroI GCCGGC 1 cut(s) 111
KroNI GCCGGC 1 cut(s) 113
Kzo9I GATC 3 cut(s) 55, 333, 355
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 3 cut(s) 125, 296, 382
MaeI CTAG 1 cut(s) 173
MaeII ACGT 2 cut(s) 72, 135
MalI GATC 3 cut(s) 57, 335, 357
MboI GATC 3 cut(s) 55, 333, 355
MboII GAAGA 7 cut(s) 34, 47, 67, 184, 288, 334, 418
MluCI AATT 4 cut(s) 126, 222, 263, 344
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 4 cut(s) 53, 104, 195, 203
MroNI GCCGGC 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 1 cut(s) 266
MspI CCGG 1 cut(s) 112
MvnI CGCG 2 cut(s) 167, 379
MwoI GCNNNNNNNGC 1 cut(s) 173
NaeI GCCGGC 1 cut(s) 113
NdeII GATC 3 cut(s) 55, 333, 355
NgoMIV GCCGGC 1 cut(s) 111
NheI GCTAGC 1 cut(s) 172
NruI TCGCGA 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PdiI GCCGGC 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 2 cut(s) 7, 381
Ple19I CGATCG 1 cut(s) 58
PsiI TTATAA 1 cut(s) 249
Psp1406I AACGTT 1 cut(s) 135
PvuI CGATCG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 167
RsaI GTAC 2 cut(s) 302, 373
RsaNI GTAC 2 cut(s) 301, 372
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 2 cut(s) 6, 380
Sau3AI GATC 3 cut(s) 55, 333, 355
ScaI AGTACT 1 cut(s) 373
SetI ASST 9 cut(s) 15, 75, 96, 138, 153, 174, 178, 214, 312
Sfr274I CTCGAG 1 cut(s) 102
SlaI CTCGAG 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 4 cut(s) 126, 222, 263, 344
SsiI CCGC 3 cut(s) 5, 146, 379
SspMI CTAG 1 cut(s) 173
TaiI ACGT 2 cut(s) 75, 138
TaqI TCGA 5 cut(s) 54, 58, 87, 103, 404
TasI AATT 4 cut(s) 126, 222, 263, 344
TatI WGTACW 1 cut(s) 371
TauI GCSGC 2 cut(s) 8, 382
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TspDTI ATGAA 2 cut(s) 134, 297
TspGWI ACGGA 1 cut(s) 222
XhoI CTCGAG 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 173
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.