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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 239, 278 |
| AfaI | GTAC | 2 cut(s) | 55, 106 |
| AgsI | TTSAA | 2 cut(s) | 14, 172 |
| Alw26I | GTCTC | 2 cut(s) | 26, 189 |
| ApeKI | GCWGC | 2 cut(s) | 73, 86 |
| ApoI | RAATTY | 2 cut(s) | 239, 278 |
| BaeGI | GKGCMC | 1 cut(s) | 8 |
| BbvI | GCAGC | 2 cut(s) | 60, 73 |
| BcoDI | GTCTC | 2 cut(s) | 26, 189 |
| BfaI | CTAG | 1 cut(s) | 78 |
| BisI | GCNGC | 2 cut(s) | 74, 87 |
| BlsI | GCNGC | 2 cut(s) | 75, 88 |
| BmcAI | AGTACT | 1 cut(s) | 55 |
| BsaI | GGTCTC | 1 cut(s) | 189 |
| Bse1I | ACTGG | 1 cut(s) | 39 |
| BseMII | CTCAG | 1 cut(s) | 262 |
| BseNI | ACTGG | 1 cut(s) | 39 |
| BseSI | GKGCMC | 1 cut(s) | 8 |
| BseXI | GCAGC | 2 cut(s) | 60, 73 |
| BsmAI | GTCTC | 2 cut(s) | 26, 189 |
| BsmBI | CGTCTC | 1 cut(s) | 26 |
| BsmI | GAATGC | 1 cut(s) | 150 |
| Bso31I | GGTCTC | 1 cut(s) | 189 |
| Bsp1286I | GDGCHC | 1 cut(s) | 8 |
| BspCNI | CTCAG | 1 cut(s) | 261 |
| BspHI | TCATGA | 1 cut(s) | 216 |
| BspTNI | GGTCTC | 1 cut(s) | 189 |
| BsrI | ACTGG | 1 cut(s) | 39 |
| Bst4CI | ACNGT | 1 cut(s) | 10 |
| BstDEI | CTNAG | 1 cut(s) | 248 |
| BstMAI | GTCTC | 2 cut(s) | 26, 189 |
| BstSLI | GKGCMC | 1 cut(s) | 8 |
| BstV1I | GCAGC | 2 cut(s) | 60, 73 |
| BtsI | GCAGTG | 1 cut(s) | 88 |
| BtsIMutI | CAGTG | 3 cut(s) | 6, 46, 88 |
| CciI | TCATGA | 1 cut(s) | 216 |
| Csp6I | GTAC | 2 cut(s) | 54, 105 |
| CviAII | CATG | 1 cut(s) | 217 |
| CviJI | RGCY | 3 cut(s) | 73, 158, 179 |
| CviKI_1 | RGCY | 3 cut(s) | 73, 158, 179 |
| CviQI | GTAC | 2 cut(s) | 54, 105 |
| DdeI | CTNAG | 1 cut(s) | 248 |
| DraI | TTTAAA | 2 cut(s) | 237, 277 |
| Eco31I | GGTCTC | 1 cut(s) | 189 |
| Esp3I | CGTCTC | 1 cut(s) | 26 |
| FaeI | CATG | 1 cut(s) | 220 |
| FaiI | YATR | 3 cut(s) | 205, 218, 265 |
| FatI | CATG | 1 cut(s) | 216 |
| Fnu4HI | GCNGC | 2 cut(s) | 74, 87 |
| Fsp4HI | GCNGC | 2 cut(s) | 74, 87 |
| FspBI | CTAG | 1 cut(s) | 78 |
| GluI | GCNGC | 2 cut(s) | 74, 87 |
| Hin1II | CATG | 1 cut(s) | 220 |
| Hpy166II | GTNNAC | 1 cut(s) | 254 |
| Hpy188III | TCNNGA | 1 cut(s) | 217 |
| Hpy8I | GTNNAC | 1 cut(s) | 254 |
| HpyCH4III | ACNGT | 1 cut(s) | 10 |
| HpyF3I | CTNAG | 1 cut(s) | 248 |
| Hsp92II | CATG | 1 cut(s) | 220 |
| LpnPI | CCDG | 4 cut(s) | 2, 52, 75, 168 |
| Lsp1109I | GCAGC | 2 cut(s) | 60, 73 |
| MaeI | CTAG | 1 cut(s) | 78 |
| MhlI | GDGCHC | 1 cut(s) | 8 |
| MluCI | AATT | 5 cut(s) | 126, 167, 239, 260, 278 |
| MmeI | TCCRAC | 1 cut(s) | 74 |
| MnlI | CCTC | 2 cut(s) | 19, 37 |
| MseI | TTAA | 3 cut(s) | 21, 236, 276 |
| MslI | CAYNNNNRTG | 1 cut(s) | 228 |
| Mva1269I | GAATGC | 1 cut(s) | 150 |
| NlaIII | CATG | 1 cut(s) | 220 |
| PagI | TCATGA | 1 cut(s) | 216 |
| PctI | GAATGC | 1 cut(s) | 150 |
| PkrI | GCNGC | 2 cut(s) | 75, 88 |
| RsaI | GTAC | 2 cut(s) | 55, 106 |
| RsaNI | GTAC | 2 cut(s) | 54, 105 |
| RseI | CAYNNNNRTG | 1 cut(s) | 228 |
| SaqAI | TTAA | 3 cut(s) | 21, 236, 276 |
| SatI | GCNGC | 2 cut(s) | 74, 87 |
| ScaI | AGTACT | 1 cut(s) | 55 |
| SduI | GDGCHC | 1 cut(s) | 8 |
| SetI | ASST | 1 cut(s) | 21 |
| SmiI | ATTTAAAT | 1 cut(s) | 277 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 228 |
| Sse9I | AATT | 5 cut(s) | 126, 167, 239, 260, 278 |
| SspI | AATATT | 1 cut(s) | 273 |
| SspMI | CTAG | 1 cut(s) | 78 |
| SwaI | ATTTAAAT | 1 cut(s) | 277 |
| TaaI | ACNGT | 1 cut(s) | 10 |
| TasI | AATT | 5 cut(s) | 126, 167, 239, 260, 278 |
| TatI | WGTACW | 2 cut(s) | 53, 104 |
| Tru1I | TTAA | 3 cut(s) | 21, 236, 276 |
| Tru9I | TTAA | 3 cut(s) | 21, 236, 276 |
| TscAI | CASTG | 3 cut(s) | 13, 46, 88 |
| TseI | GCWGC | 2 cut(s) | 73, 86 |
| TspDTI | ATGAA | 2 cut(s) | 205, 233 |
| TspRI | CASTG | 3 cut(s) | 13, 46, 88 |
| XapI | RAATTY | 2 cut(s) | 239, 278 |
| XspI | CTAG | 1 cut(s) | 78 |
| ZrmI | AGTACT | 1 cut(s) | 55 |