Rroxscaffold_5G00358620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001850
Physical Location & Seq
Reverse (-)
38733625 .. 38737827
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358620.1

Sequence Viewer

Length: 597 bp
ATGGTTGAGGATAAGGAGCTTTTTCTAGGGGAACTAAGCATCTCTTACCTCTCTTCTGCTCCAAAGAATGTCTTGAACTTGCATGCTACGAAAGAAGACTTGAAGGACTTCAGCGGAAGGAGAGAAAAATCGCTCCCAAAGTTCATAAGTCTTCAAGCGCGTGAAGAGCCTTCAAGCTGCCAAATAGCCGGTTCATCACCAACCTCAAGCCAAAGCACTCGTCACGTGTCAACACTCGTGGTCATCATACGACTCAAGATTAAGCGCCAATCGCCCTTGAATCAAGTATACCCACGAGGACAAACGACAAAGGAGGACGACGGACGAGAGGGACGCAAGCGGACGGAAGAAACCTCTCCAAAGCTCGAAGTCTCCCAAGCTCGTGAAGAACCATCAAGCTGCCAAACAGTAGGTCTCTTCACCTCACCGACTTCGGACAAGCGGGGAAATCACCTACAAGCTTGGAAGTCGTCAAACATGTGGAGGATCAATAAGCACCAAGAAAACGTGCCGATTCATCCGCCATCAAAGCCATACTCGCCACGTGACACTTCTCGTAGTTCAAGTCGATCAAGATCAAGCCTCTATAGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.31

Weight (kDa)

9.78

Isoelectric Point (pI)

75.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 160
AciI CCGC 4 cut(s) 114, 340, 442, 521
AclWI GGATC 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 94
AcvI CACGTG 2 cut(s) 226, 545
AflIII ACRYGT 2 cut(s) 225, 477
AgsI TTSAA 6 cut(s) 76, 103, 155, 174, 280, 564
AluBI AGCT 6 cut(s) 19, 177, 364, 380, 399, 461
AluI AGCT 6 cut(s) 19, 177, 364, 380, 399, 461
Alw26I GTCTC 2 cut(s) 376, 419
AlwI GGATC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 177, 399
ArsI GACNNNNNNTTYG 4 cut(s) 205, 237, 397, 429
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 2 cut(s) 160, 267
AsuHPI GGTGA 4 cut(s) 189, 412, 417, 443
BauI CACGAG 3 cut(s) 236, 294, 381
BbrPI CACGTG 2 cut(s) 226, 545
BbsI GAAGAC 2 cut(s) 102, 143
BbvI GCAGC 2 cut(s) 164, 386
BccI CCATC 2 cut(s) 400, 532
BcoDI GTCTC 2 cut(s) 376, 419
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 2 cut(s) 178, 400
BlsI GCNGC 2 cut(s) 179, 401
BmsI GCATC 1 cut(s) 48
BpiI GAAGAC 2 cut(s) 102, 143
BpuEI CTTGAG 2 cut(s) 190, 239
BsaAI YACGTR 2 cut(s) 226, 545
BsaBI GATNNNNATC 1 cut(s) 574
BsaI GGTCTC 1 cut(s) 419
Bse118I RCCGGY 1 cut(s) 188
Bse8I GATNNNNATC 1 cut(s) 574
BseGI GGATG 1 cut(s) 517
BseJI GATNNNNATC 1 cut(s) 574
BseXI GCAGC 2 cut(s) 164, 386
Bsh1236I CGCG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 345
BsmAI GTCTC 2 cut(s) 376, 419
BsmFI GGGAC 1 cut(s) 345
Bso31I GGTCTC 1 cut(s) 419
Bsp143I GATC 3 cut(s) 486, 569, 575
BspACI CCGC 4 cut(s) 114, 340, 442, 521
BspFNI CGCG 1 cut(s) 160
BspPI GGATC 1 cut(s) 494
BspQI GCTCTTC 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 419
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssMI GATC 3 cut(s) 486, 569, 575
BssNAI GTATAC 1 cut(s) 289
BssSI CACGAG 3 cut(s) 236, 294, 381
Bst1107I GTATAC 1 cut(s) 289
Bst2BI CACGAG 3 cut(s) 236, 294, 381
Bst4CI ACNGT 1 cut(s) 409
Bst6I CTCTTC 3 cut(s) 58, 159, 422
BstBAI YACGTR 2 cut(s) 226, 545
BstC8I GCNNGC 2 cut(s) 84, 338
BstDEI CTNAG 1 cut(s) 35
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 1 cut(s) 160
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 160, 267
BstKTI GATC 3 cut(s) 489, 572, 578
BstMAI GTCTC 2 cut(s) 376, 419
BstMBI GATC 3 cut(s) 486, 569, 575
BstMWI GCNNNNNNNGC 5 cut(s) 166, 271, 529, 538, 588
BstNSI RCATGY 2 cut(s) 86, 481
BstSFI CTRYAG 1 cut(s) 586
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 164, 386
BstV2I GAAGAC 2 cut(s) 102, 143
BstZ17I GTATAC 1 cut(s) 289
BtsCI GGATG 1 cut(s) 517
Cac8I GCNNGC 2 cut(s) 84, 338
CfoI GCGC 2 cut(s) 160, 267
Cfr10I RCCGGY 1 cut(s) 188
CseI GACGC 1 cut(s) 342
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 2 cut(s) 83, 478
DdeI CTNAG 1 cut(s) 35
DpnI GATC 3 cut(s) 488, 571, 577
DpnII GATC 3 cut(s) 486, 569, 575
Eam1104I CTCTTC 3 cut(s) 58, 159, 422
EarI CTCTTC 3 cut(s) 58, 159, 422
EciI GGCGGA 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 94
Eco72I CACGTG 2 cut(s) 226, 545
FaeI CATG 2 cut(s) 86, 481
FaiI YATR 7 cut(s) 84, 146, 248, 289, 479, 535, 588
FalI AAGNNNNNCTT 4 cut(s) 28, 60, 56, 88
FaqI GGGAC 1 cut(s) 345
FatI CATG 2 cut(s) 82, 477
FauI CCCGC 1 cut(s) 435
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 178, 400
FokI GGATG 1 cut(s) 504
Fsp4HI GCNGC 2 cut(s) 178, 400
FspBI CTAG 1 cut(s) 26
GlaI GCGC 2 cut(s) 159, 266
GluI GCNGC 2 cut(s) 178, 400
HaeII RGCGCY 1 cut(s) 268
HapII CCGG 1 cut(s) 189
HgaI GACGC 1 cut(s) 342
HhaI GCGC 2 cut(s) 160, 267
Hin1II CATG 2 cut(s) 86, 481
Hin6I GCGC 2 cut(s) 158, 265
HinP1I GCGC 2 cut(s) 158, 265
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 3 cut(s) 252, 280, 514
HpaII CCGG 1 cut(s) 189
HphI GGTGA 4 cut(s) 189, 412, 417, 443
Hpy166II GTNNAC 2 cut(s) 231, 289
Hpy188I TCNGA 1 cut(s) 436
Hpy188III TCNNGA 4 cut(s) 73, 256, 383, 573
Hpy8I GTNNAC 2 cut(s) 231, 289
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 97, 111, 180
HpyCH4III ACNGT 1 cut(s) 409
HpyCH4IV ACGT 3 cut(s) 225, 507, 544
HpyCH4V TGCA 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 271, 529, 538, 588
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 3 cut(s) 225, 507, 544
Hsp92II CATG 2 cut(s) 86, 481
HspAI GCGC 2 cut(s) 158, 265
Kzo9I GATC 3 cut(s) 486, 569, 575
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 3 cut(s) 16, 64, 138
LpnPI CCDG 1 cut(s) 202
Lsp1109I GCAGC 2 cut(s) 164, 386
LweI GCATC 1 cut(s) 48
MaeI CTAG 1 cut(s) 26
MaeII ACGT 3 cut(s) 225, 507, 544
MaeIII GTNAC 2 cut(s) 221, 545
MalI GATC 3 cut(s) 488, 571, 577
MboI GATC 3 cut(s) 486, 569, 575
MboII GAAGA 7 cut(s) 45, 107, 143, 176, 359, 398, 409
MlyI GAGTC 1 cut(s) 246
MnlI CCTC 9 cut(s) 59, 214, 290, 307, 322, 364, 433, 477, 593
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 5 cut(s) 166, 271, 529, 538, 588
NdeII GATC 3 cut(s) 486, 569, 575
NlaIII CATG 2 cut(s) 86, 481
NmuCI GTSAC 2 cut(s) 221, 545
NspI RCATGY 2 cut(s) 86, 481
PaeI GCATGC 1 cut(s) 86
PciI ACATGT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 2 cut(s) 280, 514
PkrI GCNGC 2 cut(s) 179, 401
PleI GAGTC 1 cut(s) 246
PmaCI CACGTG 2 cut(s) 226, 545
PmlI CACGTG 2 cut(s) 226, 545
PpsI GAGTC 1 cut(s) 246
Ppu21I YACGTR 2 cut(s) 226, 545
PscI ACATGT 1 cut(s) 477
PspCI CACGTG 2 cut(s) 226, 545
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 2 cut(s) 178, 400
Sau3AI GATC 3 cut(s) 486, 569, 575
SchI GAGTC 1 cut(s) 246
SfaNI GCATC 1 cut(s) 48
SfcI CTRYAG 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 205, 254
SmoI CTYRAG 2 cut(s) 205, 254
SphI GCATGC 1 cut(s) 86
SsiI CCGC 4 cut(s) 114, 340, 442, 521
SspMI CTAG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 409
TaiI ACGT 3 cut(s) 228, 510, 547
TaqI TCGA 2 cut(s) 366, 568
TfiI GAWTC 2 cut(s) 280, 514
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseFI GTSAC 2 cut(s) 221, 545
TseI GCWGC 2 cut(s) 177, 399
Tsp45I GTSAC 2 cut(s) 221, 545
TspDTI ATGAA 3 cut(s) 133, 183, 506
TspGWI ACGGA 2 cut(s) 336, 359
XceI RCATGY 2 cut(s) 86, 481
XmiI GTMKAC 1 cut(s) 288
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.