Rw6G020090

Belongs to the nucleosome assembly protein (NAP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001850
Physical Location & Seq
Reverse (-)
40197206 .. 40201663
4458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G020090.1

Sequence Viewer

Length: 1641 bp
ATGAGCATCGGCGCCATCGAGACCTTCGAAGGTGTGCCCTTTGGCGGCAACCTGAACCAGAAAGGCCCCGCGAAACGAATATATGACCCCATGCCAAAGCCCTTCGAGCATGACACACTGGAGAGACAGTATTTCAAGGACAGGGCAGTACTTGAAGCCGAGTTTCAACAATCTTGTCTACCTCTGCACAATCAGATTTGTGATATCGTAAATACTGTTCCTTATTTTTGGCTCAAAGCTTTGACCAACAACAAAGTGCTACGTGCCAAGATTACTGAGCACGATGAACGAGCTCTTAAGTTTCTTAGAGACATCAAGTGCTCTAAAACAGATAGCCAAAGGGGGTTTGGTTTCAAGCTTGAGTTCTTCTTTGACAGCAATCCTTTTTTTCGAAATCCTGTCCTAACAAAGACTTATGACATGATTGACGAAGATTTACCCATTTTAAGAAAAGCAATAGGGACTGAGATAGAATGGTATACCAGAACACGTTTGACACCAAATCTCAATGAGAAGAGGCTTTACGAGGGCACTAACAATTGGGGAAGTTTCTTCAACTTTTTCAGTCCACCCCAAGTCCCCAAAGTTATAGATGGAAAAGCGGCCAAGGAAATCCAAAACCTTATGGAACAAGATTATGAAATTGGGTCAGCCATTCGAGAGGTCATTCCCCATGCTGTGTCGTGGTTTGCAAAAAATTCACCTATCAGAATAACCAATCTTGCCTCAGGAGGAATTCTCAACGACCAGGCAGTGCTAGTGAGCGCTTTCAATGATAAGATGCAGATTTTAGATGGGCAGGACTCTGTGATCCTCAGGGGCCTTGGAAATATCCAGAAGAAACTTGATAAACTGGAGCATGAGTTTTTCAAAAAGAGAGGAGCACTTAGAGCCAAGTATGAAGAATTGTATGAAGCTCTCTACAACAAGCGATGCAGTATTGTAAACGGTGATGCTGGACCTGAAGGAGATTCTGATGAAGCTGCCACAAAGCAGGAGCCTGCAACCTGTAAATGCACTTGTGTAAAAGCTGTTACTGAACCAGATTTAGTTGCTTTCATACGCTCTAGAGCAAATGGAGTGCCTGGATTCTGGCTCCATGCTTTATCAAAAAATAACTTGCTAACACGAGAGATTATTGGGCGTGACACAGAAGCTCTTGCATATCTTCGAGACATCAAGGGGTCCAAGATAGATGGTGACAATAAGGGATTTAAACTAGACTTCTATTTTGAACCTAATAACTTTTTTATGAACCCGGTTTTGACCAAGACATATCATATGGTTGATGAAGGTGTACCCATTCTAGCAAAAGCAATAGGGACGGAAATAGAATGGTATCCAGGTCGTTGCTTGACAAAGAACCCCATTAAGAAGAATAGTAAGAAAGGATCTTTTCCCAAAGGAACTGAATATCGTAAAAGTTTCTTCAACTTTTTCAGTACGCCTCAAGTCCTTGAAGATATTGATGCTGTTAGAAACCAAATGAAAATAGATTATGAAATTGGGTCAACAATTCGAGACATGATCATCCCCTATGCTGTTTCTTGGTTTACGGGGGAGGCTTGGAGAAGTATGGATGAATACAGTGACACTGAGGATGATGATGAAGATGACGATGATGATGAAGATGATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAP

Protein Analysis

546

Amino Acids

62.48

Weight (kDa)

5.27

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAP PF00956 72 - 231 2.4e-44 Nucleosome assembly protein (NAP)
NAP PF00956 274 - 521 8.4e-62 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AccI GTMKAC 2 cut(s) 178, 479
AccII CGCG 1 cut(s) 71
AciI CCGC 3 cut(s) 45, 69, 602
AclWI GGATC 2 cut(s) 805, 1399
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 2 cut(s) 697, 735
AcuI CTGAAG 1 cut(s) 984
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 3 cut(s) 150, 1299, 1444
AfeI AGCGCT 1 cut(s) 766
AfiI CCNNNNNNNGG 1 cut(s) 44
AflII CTTAAG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 488
AjnI CCWGG 3 cut(s) 747, 1084, 1342
AluBI AGCT 7 cut(s) 239, 293, 358, 917, 983, 1031, 1157
AluI AGCT 7 cut(s) 239, 293, 358, 917, 983, 1031, 1157
Alw21I GWGCWC 4 cut(s) 282, 295, 323, 886
Alw26I GTCTC 5 cut(s) 14, 118, 303, 1167, 1515
AlwI GGATC 2 cut(s) 805, 1399
Aor51HI AGCGCT 1 cut(s) 766
AoxI GGCC 3 cut(s) 64, 603, 820
ApeKI GCWGC 1 cut(s) 983
ApoI RAATTY 2 cut(s) 697, 735
ArsI GACNNNNNNTTYG 2 cut(s) 1214, 1246
AspLEI GCGC 2 cut(s) 14, 767
AspS9I GGNCC 4 cut(s) 65, 820, 959, 1185
AsuC2I CCSGG 1 cut(s) 1259
AsuHPI GGTGA 3 cut(s) 693, 962, 1211
AsuII TTCGAA 2 cut(s) 27, 391
AvaII GGWCC 2 cut(s) 959, 1185
AxyI CCTNAGG 2 cut(s) 727, 815
BaeGI GKGCMC 2 cut(s) 39, 533
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BauI CACGAG 1 cut(s) 1128
Bbv12I GWGCWC 4 cut(s) 282, 295, 323, 886
BbvI GCAGC 1 cut(s) 970
BccI CCATC 4 cut(s) 23, 587, 788, 1190
BciT130I CCWGG 3 cut(s) 749, 1086, 1344
BciVI GTATCC 1 cut(s) 1350
BclI TGATCA 1 cut(s) 1527
BcnI CCSGG 1 cut(s) 1259
BcoDI GTCTC 5 cut(s) 14, 118, 303, 1167, 1515
BfaI CTAG 4 cut(s) 758, 1068, 1220, 1307
BfoI RGCGCY 2 cut(s) 15, 768
BfrI CTTAAG 1 cut(s) 296
BfuI GTATCC 1 cut(s) 1350
BisI GCNGC 3 cut(s) 46, 603, 984
BlsI GCNGC 3 cut(s) 47, 604, 985
BmcAI AGTACT 1 cut(s) 150
Bme1390I CCNGG 4 cut(s) 749, 1086, 1259, 1344
Bme18I GGWCC 2 cut(s) 959, 1185
BmgT120I GGNCC 4 cut(s) 65, 820, 959, 1185
BmiI GGNNCC 6 cut(s) 13, 67, 821, 999, 1097, 1186
BmrFI CCNGG 4 cut(s) 749, 1086, 1259, 1344
BmsI GCATC 5 cut(s) 15, 771, 923, 943, 1459
BplI GAGNNNNNCTC 2 cut(s) 723, 755
BpmI CTGGAG 2 cut(s) 140, 875
Bpu14I TTCGAA 2 cut(s) 27, 391
BpuEI CTTGAG 2 cut(s) 380, 1434
BpuMI CCSGG 1 cut(s) 1259
BsaAI YACGTR 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 606, 823
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 848, 878
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 123, 858
Bse21I CCTNAGG 2 cut(s) 727, 815
BseBI CCWGG 3 cut(s) 749, 1086, 1344
BseDI CCNNGG 2 cut(s) 606, 823
BseGI GGATG 3 cut(s) 1530, 1585, 1606
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 5 cut(s) 267, 456, 741, 829, 1587
BseNI ACTGG 2 cut(s) 123, 858
BseRI GAGGAG 1 cut(s) 894
BseSI GKGCMC 2 cut(s) 39, 533
BseXI GCAGC 1 cut(s) 970
BsgI GTGCAG 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 66, 605, 822
BshNI GGYRCC 1 cut(s) 11
BsiHKAI GWGCWC 4 cut(s) 282, 295, 323, 886
BsiSI CCGG 1 cut(s) 1259
BslFI GGGAC 3 cut(s) 475, 563, 1336
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 5 cut(s) 14, 118, 303, 1167, 1515
BsmFI GGGAC 3 cut(s) 475, 563, 1336
BsnI GGCC 3 cut(s) 66, 605, 822
Bso31I GGTCTC 1 cut(s) 14
Bsp119I TTCGAA 2 cut(s) 27, 391
Bsp1286I GDGCHC 6 cut(s) 39, 282, 295, 323, 533, 886
Bsp143I GATC 3 cut(s) 810, 1391, 1527
BspACI CCGC 3 cut(s) 45, 69, 602
BspANI GGCC 3 cut(s) 66, 605, 822
BspCNI CTCAG 5 cut(s) 268, 457, 740, 828, 1588
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 6 cut(s) 13, 67, 821, 999, 1097, 1186
BspPI GGATC 2 cut(s) 805, 1399
BspT104I TTCGAA 2 cut(s) 27, 391
BspT107I GGYRCC 1 cut(s) 11
BspTI CTTAAG 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 14
BsrI ACTGG 2 cut(s) 123, 858
BssECI CCNNGG 2 cut(s) 606, 823
BssMI GATC 3 cut(s) 810, 1391, 1527
BssNAI GTATAC 1 cut(s) 480
BssNI GRCGYC 1 cut(s) 12
BssSI CACGAG 1 cut(s) 1128
BssT1I CCWWGG 2 cut(s) 606, 823
Bst1107I GTATAC 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 1128
Bst2UI CCWGG 3 cut(s) 749, 1086, 1344
Bst4CI ACNGT 4 cut(s) 129, 217, 950, 1589
Bst6I CTCTTC 1 cut(s) 509
BstACI GRCGYC 1 cut(s) 12
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 263
BstBI TTCGAA 2 cut(s) 27, 391
BstC8I GCNNGC 1 cut(s) 1002
BstDEI CTNAG 7 cut(s) 276, 305, 465, 727, 815, 887, 1596
BstF5I GGATG 3 cut(s) 1530, 1585, 1606
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 2 cut(s) 15, 768
BstHHI GCGC 2 cut(s) 14, 767
BstKTI GATC 3 cut(s) 813, 1394, 1530
BstMAI GTCTC 5 cut(s) 14, 118, 303, 1167, 1515
BstMBI GATC 3 cut(s) 810, 1391, 1527
BstMWI GCNNNNNNNGC 2 cut(s) 106, 890
BstNI CCWGG 3 cut(s) 749, 1086, 1344
BstSCI CCNGG 4 cut(s) 747, 1084, 1257, 1342
BstSLI GKGCMC 2 cut(s) 39, 533
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 970
BstX2I RGATCY 1 cut(s) 1391
BstYI RGATCY 1 cut(s) 1391
BstZ17I GTATAC 1 cut(s) 480
Bsu36I CCTNAGG 2 cut(s) 727, 815
BsuI GTATCC 1 cut(s) 1350
BsuRI GGCC 3 cut(s) 66, 605, 822
BtgZI GCGATG 1 cut(s) 946
BtsCI GGATG 3 cut(s) 1530, 1585, 1606
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 4 cut(s) 116, 759, 1593, 1594
Cac8I GCNNGC 1 cut(s) 1002
CfoI GCGC 2 cut(s) 14, 767
Cfr13I GGNCC 4 cut(s) 65, 820, 959, 1185
Csp6I GTAC 3 cut(s) 149, 1298, 1443
CviAII CATG 7 cut(s) 91, 110, 421, 674, 860, 1100, 1525
CviQI GTAC 3 cut(s) 149, 1298, 1443
DdeI CTNAG 7 cut(s) 276, 305, 465, 727, 815, 887, 1596
DinI GGCGCC 1 cut(s) 13
DpnI GATC 3 cut(s) 812, 1393, 1529
DpnII GATC 3 cut(s) 810, 1391, 1527
DraI TTTAAA 1 cut(s) 1216
EaeI YGGCCR 1 cut(s) 603
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
Ecl136II GAGCTC 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 606, 823
Eco24I GRGCYC 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 2 cut(s) 959, 1185
Eco47III AGCGCT 1 cut(s) 766
Eco53kI GAGCTC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 984
Eco81I CCTNAGG 2 cut(s) 727, 815
EcoICRI GAGCTC 1 cut(s) 293
EcoO109I RGGNCCY 2 cut(s) 65, 820
EcoRI GAATTC 1 cut(s) 735
EcoRII CCWGG 3 cut(s) 747, 1084, 1342
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 606, 823
EcoT38I GRGCYC 1 cut(s) 295
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
ErhI CCWWGG 2 cut(s) 606, 823
FaeI CATG 7 cut(s) 94, 113, 424, 677, 863, 1103, 1528
FaqI GGGAC 3 cut(s) 475, 563, 1336
FatI CATG 7 cut(s) 90, 109, 420, 673, 859, 1099, 1524
FauI CCCGC 1 cut(s) 76
FauNDI CATATG 1 cut(s) 1281
FbaI TGATCA 1 cut(s) 1527
FblI GTMKAC 2 cut(s) 178, 479
Fnu4HI GCNGC 3 cut(s) 46, 603, 984
FokI GGATG 3 cut(s) 1517, 1592, 1613
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 3 cut(s) 46, 603, 984
FspBI CTAG 4 cut(s) 758, 1068, 1220, 1307
GlaI GCGC 2 cut(s) 13, 766
GluI GCNGC 3 cut(s) 46, 603, 984
GsuI CTGGAG 2 cut(s) 140, 875
HaeII RGCGCY 2 cut(s) 15, 768
HaeIII GGCC 3 cut(s) 66, 605, 822
HapII CCGG 1 cut(s) 1259
HhaI GCGC 2 cut(s) 14, 767
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 7 cut(s) 94, 113, 424, 677, 863, 1103, 1528
Hin6I GCGC 2 cut(s) 12, 765
HinP1I GCGC 2 cut(s) 12, 765
HincII GTYRAC 1 cut(s) 1512
HindII GTYRAC 1 cut(s) 1512
HindIII AAGCTT 2 cut(s) 237, 356
HinfI GANTC 3 cut(s) 803, 971, 1089
HpaII CCGG 1 cut(s) 1259
HphI GGTGA 3 cut(s) 693, 962, 1211
Hpy166II GTNNAC 7 cut(s) 179, 480, 569, 946, 1298, 1512, 1554
Hpy188I TCNGA 3 cut(s) 195, 710, 976
Hpy188III TCNNGA 7 cut(s) 19, 659, 729, 835, 1068, 1172, 1520
Hpy8I GTNNAC 7 cut(s) 179, 480, 569, 946, 1298, 1512, 1554
HpyAV CCTTC 5 cut(s) 23, 34, 112, 959, 1286
HpyCH4III ACNGT 4 cut(s) 129, 217, 950, 1589
HpyCH4IV ACGT 2 cut(s) 262, 490
HpyCH4V TGCA 7 cut(s) 187, 692, 784, 936, 1004, 1017, 1163
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 890
HpyF3I CTNAG 7 cut(s) 276, 305, 465, 727, 815, 887, 1596
HpySE526I ACGT 2 cut(s) 262, 490
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 7 cut(s) 94, 113, 424, 677, 863, 1103, 1528
HspAI GCGC 2 cut(s) 12, 765
KasI GGCGCC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 1527
Kzo9I GATC 3 cut(s) 810, 1391, 1527
LmnI GCTCC 4 cut(s) 856, 881, 997, 1101
Lsp1109I GCAGC 1 cut(s) 970
LweI GCATC 5 cut(s) 15, 771, 923, 943, 1459
MaeI CTAG 4 cut(s) 758, 1068, 1220, 1307
MaeII ACGT 2 cut(s) 262, 490
MaeIII GTNAC 4 cut(s) 1033, 1145, 1199, 1589
MalI GATC 3 cut(s) 812, 1393, 1529
MboI GATC 3 cut(s) 810, 1391, 1527
MfeI CAATTG 1 cut(s) 538
MflI RGATCY 1 cut(s) 1391
MhlI GDGCHC 6 cut(s) 39, 282, 295, 323, 533, 886
MluCI AATT 7 cut(s) 538, 642, 697, 735, 905, 1503, 1515
Mly113I GGCGCC 1 cut(s) 12
MlyI GAGTC 1 cut(s) 797
MseI TTAA 4 cut(s) 297, 446, 1215, 1371
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 1 cut(s) 1259
MspR9I CCNGG 4 cut(s) 749, 1086, 1259, 1344
MunI CAATTG 1 cut(s) 538
MvaI CCWGG 3 cut(s) 749, 1086, 1344
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 106, 890
NarI GGCGCC 1 cut(s) 12
NciI CCSGG 1 cut(s) 1259
NdeI CATATG 1 cut(s) 1281
NdeII GATC 3 cut(s) 810, 1391, 1527
NlaIII CATG 7 cut(s) 94, 113, 424, 677, 863, 1103, 1528
NlaIV GGNNCC 6 cut(s) 13, 67, 821, 999, 1097, 1186
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 3 cut(s) 1145, 1199, 1589
NspV TTCGAA 2 cut(s) 27, 391
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 24
PfeI GAWTC 2 cut(s) 971, 1089
PkrI GCNGC 3 cut(s) 47, 604, 985
PleI GAGTC 1 cut(s) 797
PluTI GGCGCC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 797
Ppu21I YACGTR 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 295
Psp6I CCWGG 3 cut(s) 747, 1084, 1342
PspGI CCWGG 3 cut(s) 747, 1084, 1342
PspN4I GGNNCC 6 cut(s) 13, 67, 821, 999, 1097, 1186
PspPI GGNCC 4 cut(s) 65, 820, 959, 1185
PsrI GAACNNNNNNTAC 2 cut(s) 201, 233
PsuI RGATCY 1 cut(s) 1391
RsaI GTAC 3 cut(s) 150, 1299, 1444
RsaNI GTAC 3 cut(s) 149, 1298, 1443
SacI GAGCTC 1 cut(s) 295
SaqAI TTAA 4 cut(s) 297, 446, 1215, 1371
SatI GCNGC 3 cut(s) 46, 603, 984
Sau3AI GATC 3 cut(s) 810, 1391, 1527
Sau96I GGNCC 4 cut(s) 65, 820, 959, 1185
ScaI AGTACT 1 cut(s) 150
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 4 cut(s) 749, 1086, 1259, 1344
SduI GDGCHC 6 cut(s) 39, 282, 295, 323, 533, 886
SfaNI GCATC 5 cut(s) 15, 771, 923, 943, 1459
SfoI GGCGCC 1 cut(s) 13
SfuI TTCGAA 2 cut(s) 27, 391
SinI GGWCC 2 cut(s) 959, 1185
SmlI CTYRAG 3 cut(s) 296, 359, 1449
SmoI CTYRAG 3 cut(s) 296, 359, 1449
Sse9I AATT 7 cut(s) 538, 642, 697, 735, 905, 1503, 1515
SsiI CCGC 3 cut(s) 45, 69, 602
SspDI GGCGCC 1 cut(s) 11
SspMI CTAG 4 cut(s) 758, 1068, 1220, 1307
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 4 cut(s) 747, 1084, 1257, 1342
StyI CCWWGG 2 cut(s) 606, 823
TaaI ACNGT 4 cut(s) 129, 217, 950, 1589
TaiI ACGT 2 cut(s) 265, 493
TaqI TCGA 7 cut(s) 18, 27, 105, 391, 658, 1171, 1519
TasI AATT 7 cut(s) 538, 642, 697, 735, 905, 1503, 1515
TatI WGTACW 1 cut(s) 148
TauI GCSGC 2 cut(s) 48, 605
TfiI GAWTC 2 cut(s) 971, 1089
Tru1I TTAA 4 cut(s) 297, 446, 1215, 1371
Tru9I TTAA 4 cut(s) 297, 446, 1215, 1371
TscAI CASTG 4 cut(s) 123, 759, 1594, 1600
TseFI GTSAC 3 cut(s) 1145, 1199, 1589
TseI GCWGC 1 cut(s) 983
Tsp45I GTSAC 3 cut(s) 1145, 1199, 1589
TspGWI ACGGA 1 cut(s) 1340
TspRI CASTG 4 cut(s) 123, 759, 1594, 1600
Vha464I CTTAAG 1 cut(s) 296
VpaK11BI GGWCC 2 cut(s) 959, 1185
XapI RAATTY 2 cut(s) 697, 735
XbaI TCTAGA 1 cut(s) 1067
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 178, 479
XspI CTAG 4 cut(s) 758, 1068, 1220, 1307
ZrmI AGTACT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.