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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 110 |
| AcsI | RAATTY | 1 cut(s) | 19 |
| AcuI | CTGAAG | 1 cut(s) | 90 |
| AgsI | TTSAA | 3 cut(s) | 18, 60, 142 |
| AjnI | CCWGG | 1 cut(s) | 185 |
| AluBI | AGCT | 1 cut(s) | 80 |
| AluI | AGCT | 1 cut(s) | 80 |
| ApoI | RAATTY | 1 cut(s) | 19 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 68 |
| AspS9I | GGNCC | 1 cut(s) | 183 |
| AsuHPI | GGTGA | 1 cut(s) | 64 |
| AvaII | GGWCC | 1 cut(s) | 183 |
| BanI | GGYRCC | 1 cut(s) | 110 |
| BbvCI | CCTCAGC | 1 cut(s) | 81 |
| BceAI | ACGGC | 1 cut(s) | 81 |
| BciT130I | CCWGG | 1 cut(s) | 187 |
| Bme1390I | CCNGG | 1 cut(s) | 187 |
| Bme18I | GGWCC | 1 cut(s) | 183 |
| BmgT120I | GGNCC | 1 cut(s) | 183 |
| BmiI | GGNNCC | 2 cut(s) | 95, 112 |
| BmrFI | CCNGG | 1 cut(s) | 187 |
| BmsI | GCATC | 1 cut(s) | 181 |
| Bpu10I | CCTNAGC | 1 cut(s) | 81 |
| BsaJI | CCNNGG | 1 cut(s) | 186 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 141, 171 |
| Bse1I | ACTGG | 1 cut(s) | 121 |
| BseBI | CCWGG | 1 cut(s) | 187 |
| BseDI | CCNNGG | 1 cut(s) | 186 |
| BseGI | GGATG | 1 cut(s) | 196 |
| BseMII | CTCAG | 1 cut(s) | 72 |
| BseNI | ACTGG | 1 cut(s) | 121 |
| BseRI | GAGGAG | 1 cut(s) | 106 |
| BshNI | GGYRCC | 1 cut(s) | 110 |
| BslFI | GGGAC | 1 cut(s) | 162 |
| BsmFI | GGGAC | 1 cut(s) | 162 |
| BspCNI | CTCAG | 1 cut(s) | 73 |
| BspLI | GGNNCC | 2 cut(s) | 95, 112 |
| BspT107I | GGYRCC | 1 cut(s) | 110 |
| BsrI | ACTGG | 1 cut(s) | 121 |
| BssECI | CCNNGG | 1 cut(s) | 186 |
| Bst2UI | CCWGG | 1 cut(s) | 187 |
| BstDEI | CTNAG | 1 cut(s) | 81 |
| BstF5I | GGATG | 1 cut(s) | 196 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 32 |
| BstNI | CCWGG | 1 cut(s) | 187 |
| BstSCI | CCNGG | 1 cut(s) | 185 |
| BtsCI | GGATG | 1 cut(s) | 196 |
| Cfr13I | GGNCC | 1 cut(s) | 183 |
| CviJI | RGCY | 3 cut(s) | 26, 80, 96 |
| CviKI_1 | RGCY | 3 cut(s) | 26, 80, 96 |
| DdeI | CTNAG | 1 cut(s) | 81 |
| Eco47I | GGWCC | 1 cut(s) | 183 |
| Eco57I | CTGAAG | 1 cut(s) | 90 |
| EcoRII | CCWGG | 1 cut(s) | 185 |
| FaiI | YATR | 2 cut(s) | 33, 126 |
| FaqI | GGGAC | 1 cut(s) | 162 |
| HinfI | GANTC | 1 cut(s) | 39 |
| HphI | GGTGA | 1 cut(s) | 64 |
| Hpy188III | TCNNGA | 1 cut(s) | 160 |
| HpyCH4V | TGCA | 2 cut(s) | 35, 133 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 32 |
| HpyF3I | CTNAG | 1 cut(s) | 81 |
| LmnI | GCTCC | 1 cut(s) | 93 |
| LpnPI | CCDG | 6 cut(s) | 21, 93, 131, 134, 172, 173 |
| LweI | GCATC | 1 cut(s) | 181 |
| MluCI | AATT | 2 cut(s) | 19, 49 |
| MnlI | CCTC | 4 cut(s) | 37, 76, 84, 167 |
| MroXI | GAANNNNTTC | 1 cut(s) | 68 |
| MseI | TTAA | 1 cut(s) | 171 |
| MslI | CAYNNNNRTG | 1 cut(s) | 123 |
| MspR9I | CCNGG | 1 cut(s) | 187 |
| MvaI | CCWGG | 1 cut(s) | 187 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 32 |
| NlaIV | GGNNCC | 2 cut(s) | 95, 112 |
| PdmI | GAANNNNTTC | 1 cut(s) | 68 |
| PfeI | GAWTC | 1 cut(s) | 39 |
| Psp6I | CCWGG | 1 cut(s) | 185 |
| PspGI | CCWGG | 1 cut(s) | 185 |
| PspN4I | GGNNCC | 2 cut(s) | 95, 112 |
| PspPI | GGNCC | 1 cut(s) | 183 |
| RseI | CAYNNNNRTG | 1 cut(s) | 123 |
| SaqAI | TTAA | 1 cut(s) | 171 |
| Sau96I | GGNCC | 1 cut(s) | 183 |
| ScrFI | CCNGG | 1 cut(s) | 187 |
| SetI | ASST | 2 cut(s) | 82, 159 |
| SfaNI | GCATC | 1 cut(s) | 181 |
| SgeI | CNNG | 5 cut(s) | 48, 120, 133, 158, 172 |
| SinI | GGWCC | 1 cut(s) | 183 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 123 |
| Sse9I | AATT | 2 cut(s) | 19, 49 |
| StyD4I | CCNGG | 1 cut(s) | 185 |
| TasI | AATT | 2 cut(s) | 19, 49 |
| TfiI | GAWTC | 1 cut(s) | 39 |
| Tru1I | TTAA | 1 cut(s) | 171 |
| Tru9I | TTAA | 1 cut(s) | 171 |
| TspDTI | ATGAA | 2 cut(s) | 77, 141 |
| VpaK11BI | GGWCC | 1 cut(s) | 183 |
| XapI | RAATTY | 1 cut(s) | 19 |
| XmnI | GAANNNNTTC | 1 cut(s) | 68 |