Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0001344

Physical Location & Seq

Genomic Coordinates Forward (+)

29026713 .. 29029356

Gene Structure 2644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG289600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGAATTTCCTACCGCCGCGACTTGGAGTCTGGAGACTTGGACTCTTGGAGCACAGAGGCGCAGAGCAGTCCGACTCAGCGACCCGGCGATCTGCAAAAGTGCAAGGCTGGAAGCCTGGAACCAGCAACCCATCTCCGGTCTCCGAGCTCCCCACCGCCACCAGCAACCCGTCTGGGCCGTCTGGCTGTCTCCGTGGTTCTTGCATTGCTGAAAGTCAAGACACTGGGTCTTCGGCAACCTCATCAAGATTTGCAGTTGGCCAGTTGTCTACTTATCTGGCCTCAGGCCTGCAATTTCAATATCATCAACAGGCACATAAGGTGGATTGCATTGTGTTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTGGTTGTTAACACACGACTCATGAAAAATATAGTGCTTCGAGAATTCTGTGAACTTGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGGCCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAGCCGGGCCAGAAGCGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCAGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCACTCGATGCAGAAGACTACCTAGCAGAAGCTGAAGGGCTGGCTCAAACTGGGATTCTAGTAGCCATAGATGGAGAAGTGGCTGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTAGAAGCATAATGGTGACAGGTGACAATTGGGGAATTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGCGAAACCTGATCACCAGAAAGCAGAGAAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGTGATGGTGGGAGATGGCATCAATGACTCACCAGCACTTGTGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTAACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGATATTAATTTCTGATGGAAATGACATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

363

Amino Acids

38.25

Weight (kDa)

4.98

Isoelectric Point (pI)

29.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	108 - 332	7.5e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001344)

Species	Orthologous Gene IDs
malus_domestica	MD02G1192500.v1.1 MD03G1138700.v1.1 MD03G1180400.v1.1 MD03G1181400.v1.1 MD03G1182600.v1.1
pyrus_communis	pycom02g15570 pycom02g15620
rosa_chinensis	RchiOBHm_Chr2g0138301 RchiOBHm_Chr7g0198201
rosa_laevigata	RLG00000003887 RLG00000035827
rosa_multiflora	Rmu_sc0000221.1_g000001 Rmu_sc0002263.1_g000023 Rmu_sc0003465.1_g000045
rosa_roxburghii	Rroxscaffold_2G00106950 Rroxscaffold_2G00141500 Rroxscaffold_3G00232230 Rroxscaffold_5G00354760 Rroxscaffold_5G00354830
rosa_rugosa	Rorug01G0072400 Rorug01G0080400 Rorug02G0391900 Rorug03G0335000 Rorug05G0277500 Rorug06G0193000
rosa_samantha	Rh1AG099400 Rh1DG291600 Rh3BG287400 Rh3BG289600 Rh4BG446000 Rh4CG463300 Rh4DG336400 Rh5BG050800 Rh5BG085000 Rh5CG336100 Rh5CG336700 Rh5DG044200 Rh6BG050400 Rh7AG169000 Rh7AG169400 Rh7BG078400 Rh7BG172600 Rh7BG434400 Rh7BG434500 Rh7CG179100 Rh7CG468500 Rh7CG479100 Rh7DG171900 Rh7DG271300
rosa_wichuraiana	Rw1G013030 Rw3G006160 Rw4G015230 Rw5G030130 Rw6G044730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	269
AccII	CGCG	1 cut(s)	19
AciI	CCGC	4 cut(s)	14, 17, 156, 989
AclWI	GGATC	1 cut(s)	737
AcoI	YGGCCR	2 cut(s)	259, 465
AcsI	RAATTY	4 cut(s)	4, 416, 494, 838
AcuI	CTGAAG	2 cut(s)	693, 915
AdeI	CACNNNGTG	1 cut(s)	565
AfiI	CCNNNNNNNGG	2 cut(s)	23, 136
AgsI	TTSAA	1 cut(s)	299
AhdI	GACNNNNNGTC	2 cut(s)	26, 226
AjnI	CCWGG	2 cut(s)	115, 754
AleI	CACNNNNGTG	1 cut(s)	944
AluBI	AGCT	4 cut(s)	148, 440, 612, 671
AluI	AGCT	4 cut(s)	148, 440, 612, 671
Alw21I	GWGCWC	3 cut(s)	54, 150, 763
Alw26I	GTCTC	3 cut(s)	28, 145, 194
AlwI	GGATC	1 cut(s)	737
AlwNI	CAGNNNCTG	1 cut(s)	671
AoxI	GGCC	7 cut(s)	176, 259, 279, 286, 465, 471, 524
ApeKI	GCWGC	3 cut(s)	434, 437, 1040
ApoI	RAATTY	4 cut(s)	4, 416, 494, 838
AseI	ATTAAT	1 cut(s)	1067
AspLEI	GCGC	2 cut(s)	62, 535
AspS9I	GGNCC	3 cut(s)	176, 524, 622
AsuC2I	CCSGG	2 cut(s)	85, 523
AsuHPI	GGTGA	6 cut(s)	577, 823, 830, 887, 958, 966
AvaII	GGWCC	1 cut(s)	622
AxyI	CCTNAGG	1 cut(s)	283
BalI	TGGCCA	2 cut(s)	261, 467
BanII	GRGCYC	1 cut(s)	150
BbsI	GAAGAC	2 cut(s)	222, 660
Bbv12I	GWGCWC	3 cut(s)	54, 150, 763
BbvCI	CCTCAGC	1 cut(s)	441
BbvI	GCAGC	3 cut(s)	421, 449, 1052
BccI	CCATC	6 cut(s)	139, 610, 704, 943, 953, 1070
BceAI	ACGGC	1 cut(s)	163
BcgI	CGANNNNNNTGC	2 cut(s)	319, 353
BciT130I	CCWGG	2 cut(s)	117, 756
BclI	TGATCA	1 cut(s)	892
BcnI	CCSGG	2 cut(s)	85, 523
BcoDI	GTCTC	3 cut(s)	28, 145, 194
BfaI	CTAG	3 cut(s)	662, 698, 731
BfmI	CTRYAG	1 cut(s)	435
BisI	GCNGC	5 cut(s)	17, 435, 438, 990, 1041
BlsI	GCNGC	5 cut(s)	18, 436, 439, 991, 1042
Bme1390I	CCNGG	4 cut(s)	85, 117, 523, 756
Bme18I	GGWCC	1 cut(s)	622
BmeRI	GACNNNNNGTC	2 cut(s)	26, 226
BmgT120I	GGNCC	3 cut(s)	176, 524, 622
BmiI	GGNNCC	1 cut(s)	121
BmrFI	CCNGG	4 cut(s)	85, 117, 523, 756
BmrI	ACTGGG	2 cut(s)	234, 699
BmsI	GCATC	2 cut(s)	637, 972
BmuI	ACTGGG	2 cut(s)	234, 699
BpiI	GAAGAC	2 cut(s)	222, 660
BpmI	CTGGAG	2 cut(s)	52, 744
Bpu10I	CCTNAGC	1 cut(s)	441
BpuEI	CTTGAG	2 cut(s)	747, 767
BpuMI	CCSGG	2 cut(s)	85, 523
BsaI	GGTCTC	1 cut(s)	145
BsaJI	CCNNGG	3 cut(s)	193, 462, 468
BsaWI	WCCGGW	1 cut(s)	136
BsaXI	ACNNNNNCTCC	2 cut(s)	530, 560
Bsc4I	CCNNNNNNNGG	2 cut(s)	23, 136
Bse1I	ACTGG	5 cut(s)	229, 262, 378, 559, 694
Bse21I	CCTNAGG	1 cut(s)	283
Bse3DI	GCAATG	3 cut(s)	204, 630, 1026
BseBI	CCWGG	2 cut(s)	117, 756
BseDI	CCNNGG	3 cut(s)	193, 462, 468
BseLI	CCNNNNNNNGG	2 cut(s)	23, 136
BseMI	GCAATG	3 cut(s)	204, 630, 1026
BseMII	CTCAG	3 cut(s)	90, 297, 432
BseNI	ACTGG	5 cut(s)	229, 262, 378, 559, 694
BseXI	GCAGC	3 cut(s)	421, 449, 1052
Bsh1236I	CGCG	1 cut(s)	19
BshFI	GGCC	7 cut(s)	178, 261, 281, 288, 467, 473, 526
BsiHKAI	GWGCWC	3 cut(s)	54, 150, 763
BsiSI	CCGG	3 cut(s)	85, 137, 522
BslFI	GGGAC	1 cut(s)	364
BslI	CCNNNNNNNGG	2 cut(s)	23, 136
BsmAI	GTCTC	3 cut(s)	28, 145, 194
BsmFI	GGGAC	1 cut(s)	364
BsnI	GGCC	7 cut(s)	178, 261, 281, 288, 467, 473, 526
Bso31I	GGTCTC	1 cut(s)	145
Bsp1286I	GDGCHC	3 cut(s)	54, 150, 763
Bsp143I	GATC	3 cut(s)	89, 742, 892
BspACI	CCGC	4 cut(s)	14, 17, 156, 989
BspANI	GGCC	7 cut(s)	178, 261, 281, 288, 467, 473, 526
BspCNI	CTCAG	3 cut(s)	89, 296, 433
BspFNI	CGCG	1 cut(s)	19
BspHI	TCATGA	1 cut(s)	393
BspLI	GGNNCC	1 cut(s)	121
BspMAI	CTGCAG	1 cut(s)	439
BspPI	GGATC	1 cut(s)	737
BspTNI	GGTCTC	1 cut(s)	145
BsrDI	GCAATG	3 cut(s)	204, 630, 1026
BsrI	ACTGG	5 cut(s)	229, 262, 378, 559, 694
BssECI	CCNNGG	3 cut(s)	193, 462, 468
BssMI	GATC	3 cut(s)	89, 742, 892
BssT1I	CCWWGG	2 cut(s)	462, 468
Bst2UI	CCWGG	2 cut(s)	117, 756
Bst4CI	ACNGT	3 cut(s)	452, 871, 943
Bst6I	CTCTTC	1 cut(s)	504
BstAPI	GCANNNNNTGC	1 cut(s)	1013
BstC8I	GCNNGC	4 cut(s)	290, 681, 927, 1018
BstDEI	CTNAG	3 cut(s)	76, 283, 441
BstDSI	CCRYGG	1 cut(s)	193
BstFNI	CGCG	1 cut(s)	19
BstHHI	GCGC	2 cut(s)	62, 535
BstKTI	GATC	3 cut(s)	92, 745, 895
BstMAI	GTCTC	3 cut(s)	28, 145, 194
BstMBI	GATC	3 cut(s)	89, 742, 892
BstMWI	GCNNNNNNNGC	4 cut(s)	532, 986, 1013, 1037
BstNI	CCWGG	2 cut(s)	117, 756
BstSCI	CCNGG	4 cut(s)	83, 115, 521, 754
BstSFI	CTRYAG	1 cut(s)	435
BstUI	CGCG	1 cut(s)	19
BstV1I	GCAGC	3 cut(s)	421, 449, 1052
BstV2I	GAAGAC	2 cut(s)	222, 660
Bsu36I	CCTNAGG	1 cut(s)	283
BsuRI	GGCC	7 cut(s)	178, 261, 281, 288, 467, 473, 526
BtgI	CCRYGG	1 cut(s)	193
BtsIMutI	CAGTG	5 cut(s)	222, 371, 457, 746, 939
Cac8I	GCNNGC	4 cut(s)	290, 681, 927, 1018
CaiI	CAGNNNCTG	1 cut(s)	671
CciI	TCATGA	1 cut(s)	393
CfoI	GCGC	2 cut(s)	62, 535
Cfr13I	GGNCC	3 cut(s)	176, 524, 622
CsiI	ACCWGGT	1 cut(s)	754
CviAII	CATG	2 cut(s)	394, 536
DdeI	CTNAG	3 cut(s)	76, 283, 441
DpnI	GATC	3 cut(s)	91, 744, 894
DpnII	GATC	3 cut(s)	89, 742, 892
DraIII	CACNNNGTG	1 cut(s)	565
DriI	GACNNNNNGTC	2 cut(s)	26, 226
EaeI	YGGCCR	2 cut(s)	259, 465
Eam1104I	CTCTTC	1 cut(s)	504
Eam1105I	GACNNNNNGTC	2 cut(s)	26, 226
EarI	CTCTTC	1 cut(s)	504
Ecl136II	GAGCTC	1 cut(s)	148
Eco130I	CCWWGG	2 cut(s)	462, 468
Eco147I	AGGCCT	1 cut(s)	288
Eco24I	GRGCYC	1 cut(s)	150
Eco31I	GGTCTC	1 cut(s)	145
Eco47I	GGWCC	1 cut(s)	622
Eco53kI	GAGCTC	1 cut(s)	148
Eco57I	CTGAAG	2 cut(s)	693, 915
Eco81I	CCTNAGG	1 cut(s)	283
EcoICRI	GAGCTC	1 cut(s)	148
EcoRI	GAATTC	1 cut(s)	416
EcoRII	CCWGG	2 cut(s)	115, 754
EcoT14I	CCWWGG	2 cut(s)	462, 468
EcoT38I	GRGCYC	1 cut(s)	150
ErhI	CCWWGG	2 cut(s)	462, 468
FaeI	CATG	2 cut(s)	397, 539
FaiI	YATR	9 cut(s)	318, 395, 404, 486, 537, 707, 779, 806, 875
FaqI	GGGAC	1 cut(s)	364
FatI	CATG	2 cut(s)	393, 535
FbaI	TGATCA	1 cut(s)	892
FblI	GTMKAC	1 cut(s)	269
Fnu4HI	GCNGC	5 cut(s)	17, 435, 438, 990, 1041
FriOI	GRGCYC	1 cut(s)	150
Fsp4HI	GCNGC	5 cut(s)	17, 435, 438, 990, 1041
FspBI	CTAG	3 cut(s)	662, 698, 731
GlaI	GCGC	2 cut(s)	61, 534
GluI	GCNGC	5 cut(s)	17, 435, 438, 990, 1041
GsuI	CTGGAG	2 cut(s)	52, 744
HaeIII	GGCC	7 cut(s)	178, 261, 281, 288, 467, 473, 526
HapII	CCGG	3 cut(s)	85, 137, 522
HhaI	GCGC	2 cut(s)	62, 535
Hin1II	CATG	2 cut(s)	397, 539
Hin6I	GCGC	2 cut(s)	60, 533
HinP1I	GCGC	2 cut(s)	60, 533
HincII	GTYRAC	2 cut(s)	382, 448
HindII	GTYRAC	2 cut(s)	382, 448
HinfI	GANTC	8 cut(s)	27, 42, 74, 352, 390, 514, 694, 971
HpaI	GTTAAC	2 cut(s)	382, 448
HpaII	CCGG	3 cut(s)	85, 137, 522
HphI	GGTGA	6 cut(s)	577, 823, 830, 887, 958, 966
Hpy166II	GTNNAC	6 cut(s)	270, 382, 425, 448, 455, 622
Hpy188I	TCNGA	5 cut(s)	73, 145, 500, 742, 1075
Hpy188III	TCNNGA	6 cut(s)	31, 218, 246, 394, 413, 764
Hpy8I	GTNNAC	6 cut(s)	270, 382, 425, 448, 455, 622
HpyAV	CCTTC	3 cut(s)	562, 668, 910
HpyCH4III	ACNGT	3 cut(s)	452, 871, 943
HpyF10VI	GCNNNNNNNGC	4 cut(s)	532, 986, 1013, 1037
HpyF3I	CTNAG	3 cut(s)	76, 283, 441
Hsp92II	CATG	2 cut(s)	397, 539
HspAI	GCGC	2 cut(s)	60, 533
Ksp22I	TGATCA	1 cut(s)	892
KspAI	GTTAAC	2 cut(s)	382, 448
Kzo9I	GATC	3 cut(s)	89, 742, 892
LmnI	GCTCC	2 cut(s)	49, 153
Lsp1109I	GCAGC	3 cut(s)	421, 449, 1052
LweI	GCATC	2 cut(s)	637, 972
MabI	ACCWGGT	1 cut(s)	754
MaeI	CTAG	3 cut(s)	662, 698, 731
MaeIII	GTNAC	3 cut(s)	557, 811, 818
MalI	GATC	3 cut(s)	91, 744, 894
MboI	GATC	3 cut(s)	89, 742, 892
MboII	GAAGA	5 cut(s)	222, 515, 521, 665, 1072
MfeI	CAATTG	4 cut(s)	360, 823, 1008, 1032
MhlI	GDGCHC	3 cut(s)	54, 150, 763
MlsI	TGGCCA	2 cut(s)	261, 467
MluNI	TGGCCA	2 cut(s)	261, 467
MlyI	GAGTC	6 cut(s)	36, 36, 68, 346, 384, 965
MmeI	TCCRAC	1 cut(s)	96
MnlI	CCTC	6 cut(s)	50, 250, 292, 436, 556, 1030
Mox20I	TGGCCA	2 cut(s)	261, 467
MscI	TGGCCA	2 cut(s)	261, 467
MseI	TTAA	3 cut(s)	381, 447, 1067
MslI	CAYNNNNRTG	2 cut(s)	809, 944
Msp20I	TGGCCA	2 cut(s)	261, 467
MspA1I	CMGCKG	1 cut(s)	440
MspI	CCGG	3 cut(s)	85, 137, 522
MspR9I	CCNGG	4 cut(s)	85, 117, 523, 756
MunI	CAATTG	4 cut(s)	360, 823, 1008, 1032
MvaI	CCWGG	2 cut(s)	117, 756
MvnI	CGCG	1 cut(s)	19
MwoI	GCNNNNNNNGC	4 cut(s)	532, 986, 1013, 1037
NciI	CCSGG	2 cut(s)	85, 523
NdeII	GATC	3 cut(s)	89, 742, 892
NlaIII	CATG	2 cut(s)	397, 539
NlaIV	GGNNCC	1 cut(s)	121
NmuCI	GTSAC	3 cut(s)	557, 811, 818
OliI	CACNNNNGTG	1 cut(s)	944
PagI	TCATGA	1 cut(s)	393
PceI	AGGCCT	1 cut(s)	288
PfeI	GAWTC	2 cut(s)	514, 694
PkrI	GCNGC	5 cut(s)	18, 436, 439, 991, 1042
PleI	GAGTC	6 cut(s)	35, 36, 68, 346, 384, 965
PpsI	GAGTC	6 cut(s)	35, 36, 68, 346, 384, 965
PshBI	ATTAAT	1 cut(s)	1067
Psp124BI	GAGCTC	1 cut(s)	150
Psp6I	CCWGG	2 cut(s)	115, 754
PspGI	CCWGG	2 cut(s)	115, 754
PspN4I	GGNNCC	1 cut(s)	121
PspPI	GGNCC	3 cut(s)	176, 524, 622
PstI	CTGCAG	1 cut(s)	439
PstNI	CAGNNNCTG	1 cut(s)	671
PvuII	CAGCTG	1 cut(s)	440
RseI	CAYNNNNRTG	2 cut(s)	809, 944
SacI	GAGCTC	1 cut(s)	150
SaqAI	TTAA	3 cut(s)	381, 447, 1067
SatI	GCNGC	5 cut(s)	17, 435, 438, 990, 1041
Sau3AI	GATC	3 cut(s)	89, 742, 892
Sau96I	GGNCC	3 cut(s)	176, 524, 622
SchI	GAGTC	6 cut(s)	36, 36, 68, 346, 384, 965
ScrFI	CCNGG	4 cut(s)	85, 117, 523, 756
SduI	GDGCHC	3 cut(s)	54, 150, 763
SexAI	ACCWGGT	1 cut(s)	754
SfaNI	GCATC	2 cut(s)	637, 972
SfcI	CTRYAG	1 cut(s)	435
SinI	GGWCC	1 cut(s)	622
SmiMI	CAYNNNNRTG	2 cut(s)	809, 944
SmlI	CTYRAG	2 cut(s)	762, 782
SmoI	CTYRAG	2 cut(s)	762, 782
SseBI	AGGCCT	1 cut(s)	288
SsiI	CCGC	4 cut(s)	14, 17, 156, 989
SspMI	CTAG	3 cut(s)	662, 698, 731
SstI	GAGCTC	1 cut(s)	150
StuI	AGGCCT	1 cut(s)	288
StyD4I	CCNGG	4 cut(s)	83, 115, 521, 754
StyI	CCWWGG	2 cut(s)	462, 468
TaaI	ACNGT	3 cut(s)	452, 871, 943
TaqI	TCGA	3 cut(s)	339, 412, 645
TauI	GCSGC	2 cut(s)	19, 992
TfiI	GAWTC	2 cut(s)	514, 694
Tru1I	TTAA	3 cut(s)	381, 447, 1067
Tru9I	TTAA	3 cut(s)	381, 447, 1067
TscAI	CASTG	5 cut(s)	229, 378, 457, 753, 946
TseFI	GTSAC	3 cut(s)	557, 811, 818
TseI	GCWGC	3 cut(s)	434, 437, 1040
Tsp45I	GTSAC	3 cut(s)	557, 811, 818
TspDTI	ATGAA	6 cut(s)	17, 410, 522, 806, 867, 1073
TspGWI	ACGGA	1 cut(s)	182
TspRI	CASTG	5 cut(s)	229, 378, 457, 753, 946
VpaK11BI	GGWCC	1 cut(s)	622
VspI	ATTAAT	1 cut(s)	1067
XapI	RAATTY	4 cut(s)	4, 416, 494, 838
XmiI	GTMKAC	1 cut(s)	269
XspI	CTAG	3 cut(s)	662, 698, 731

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.