Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0001344

Physical Location & Seq

Genomic Coordinates Reverse (-)

3659322 .. 3661870

Gene Structure 2549 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG044200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGATGAGCTTGTTGGTATGAATTTCCTACCGCCGCGACTTGGAGTCTGGAGACTTGGACTCTTGGAGCACAGAGGCGCAGAGCAGTCCGACTCAGCGACCCGGCGATCTGCAAAAGTGCAAGGCTGGAACCAGCAACCCATCTCCGGTAGTCCGGTCTCCGAGCTCCCCACCGCCACCAGCAACCCGTCTGGGCCGTCTGGCTGTCTCCGTGGTTCTTGCATTGCTGAAAGTCAAGACACTGGGTCTTTGGCAACGTCATCAAGATTTGCAGTTGGCCAGTTGTCTACTTATCTGGCCTCAGGCCTGCAATTTCAATATCACCAACAGGTGGACTGCATTGTGTTCGACAAGACAGGGACTCTTACAATTGGGAAGCCACTGGTTGTTAACACACGACTCATGAAAAATATAGTGCTTCGAGAATTCTATGAACTTGTTGCTGCAGCTGAGGTTAACAGTGAACACCCCTTGGCCAAGACCATTGTTGAGTATGCCAAAAAATTCAGAGAAGATGAAGAGAATCCAGCCGGGCCAGAAGCGCATGACTTTGCCTCCATTACTGGTCACGGGGTGAAGGCTATTGTTCAGGGCAGGGAAATAATTGTGGGGAACAAGAGCTTGATGGTGGACCAGAACATTGCAGTTCCACTCGATGCAGAAGACTACCTAGCAGAAGCTGAAGGGCTGGCTCAAACTGGGATTCTAGTAGCCATAGATGGAGAAGTGGCTGGAGTTCTAGCAATATCTGATCCACTGAAACCAGGTGCTCAAGAAGTAATTACCATACTCAAGTCAATGAATGTTAGAAGCATAATGGTGACAGGTGACAATTGGGGAATTGCAAATTCTATTGCCAATGAAGTTGGTATTGATACTGTTATAGCCGAAGCGAAACCTGATCACCAGAAAGCAGAGAAAGTGAAGGAATTGCAGGCTTCAGGCTACACTGTGGCGATGGTGGGAGATGGCATCAATGACTCACCAGCACTTGTGGCGGCAGATGTAGGAATGGCAATTGGTGCTGGCACTAACATTGCAATTGAGGCAGCAGACATTGTGCTAATGAAGAGTAGGTTTCCTCTTTATTTTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

365

Amino Acids

38.77

Weight (kDa)

4.87

Isoelectric Point (pI)

31.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	111 - 335	5.2e-32	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001344)

Species	Orthologous Gene IDs
malus_domestica	MD02G1192500.v1.1 MD03G1138700.v1.1 MD03G1180400.v1.1 MD03G1181400.v1.1 MD03G1182600.v1.1
pyrus_communis	pycom02g15570 pycom02g15620
rosa_chinensis	RchiOBHm_Chr2g0138301 RchiOBHm_Chr7g0198201
rosa_laevigata	RLG00000003887 RLG00000035827
rosa_multiflora	Rmu_sc0000221.1_g000001 Rmu_sc0002263.1_g000023 Rmu_sc0003465.1_g000045
rosa_roxburghii	Rroxscaffold_2G00106950 Rroxscaffold_2G00141500 Rroxscaffold_3G00232230 Rroxscaffold_5G00354760 Rroxscaffold_5G00354830
rosa_rugosa	Rorug01G0072400 Rorug01G0080400 Rorug02G0391900 Rorug03G0335000 Rorug05G0277500 Rorug06G0193000
rosa_samantha	Rh1AG099400 Rh1DG291600 Rh3BG287400 Rh3BG289600 Rh4BG446000 Rh4CG463300 Rh4DG336400 Rh5BG050800 Rh5BG085000 Rh5CG336100 Rh5CG336700 Rh5DG044200 Rh6BG050400 Rh7AG169000 Rh7AG169400 Rh7BG078400 Rh7BG172600 Rh7BG434400 Rh7BG434500 Rh7CG179100 Rh7CG468500 Rh7CG479100 Rh7DG171900 Rh7DG271300
rosa_wichuraiana	Rw1G013030 Rw3G006160 Rw4G015230 Rw5G030130 Rw6G044730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	331
AccI	GTMKAC	1 cut(s)	287
AccII	CGCG	1 cut(s)	37
AciI	CCGC	4 cut(s)	32, 35, 174, 998
AclWI	GGATC	1 cut(s)	746
AcoI	YGGCCR	2 cut(s)	277, 474
AcsI	RAATTY	4 cut(s)	22, 425, 503, 847
AcuI	CTGAAG	2 cut(s)	702, 924
AdeI	CACNNNGTG	1 cut(s)	574
AfiI	CCNNNNNNNGG	3 cut(s)	41, 146, 331
AgsI	TTSAA	1 cut(s)	317
AhdI	GACNNNNNGTC	2 cut(s)	44, 244
AjnI	CCWGG	1 cut(s)	763
AluBI	AGCT	5 cut(s)	10, 166, 449, 621, 680
AluI	AGCT	5 cut(s)	10, 166, 449, 621, 680
Alw21I	GWGCWC	3 cut(s)	72, 168, 772
Alw26I	GTCTC	3 cut(s)	46, 163, 212
AlwI	GGATC	1 cut(s)	746
AlwNI	CAGNNNCTG	1 cut(s)	680
AoxI	GGCC	6 cut(s)	194, 277, 297, 304, 474, 533
ApeKI	GCWGC	3 cut(s)	443, 446, 1049
ApoI	RAATTY	4 cut(s)	22, 425, 503, 847
AspLEI	GCGC	2 cut(s)	80, 544
AspS9I	GGNCC	3 cut(s)	194, 533, 631
AsuC2I	CCSGG	2 cut(s)	103, 532
AsuHPI	GGTGA	6 cut(s)	314, 586, 832, 839, 896, 975
AvaII	GGWCC	1 cut(s)	631
AxyI	CCTNAGG	1 cut(s)	301
BalI	TGGCCA	2 cut(s)	279, 476
BanII	GRGCYC	1 cut(s)	168
BbsI	GAAGAC	1 cut(s)	669
Bbv12I	GWGCWC	3 cut(s)	72, 168, 772
BbvCI	CCTCAGC	1 cut(s)	450
BbvI	GCAGC	3 cut(s)	430, 458, 1061
BccI	CCATC	5 cut(s)	149, 619, 713, 952, 962
BceAI	ACGGC	1 cut(s)	181
BcgI	CGANNNNNNTGC	2 cut(s)	328, 362
BciT130I	CCWGG	1 cut(s)	765
BclI	TGATCA	1 cut(s)	901
BcnI	CCSGG	2 cut(s)	103, 532
BcoDI	GTCTC	3 cut(s)	46, 163, 212
BfaI	CTAG	3 cut(s)	671, 707, 740
BfmI	CTRYAG	1 cut(s)	444
BisI	GCNGC	5 cut(s)	35, 444, 447, 999, 1050
BlsI	GCNGC	5 cut(s)	36, 445, 448, 1000, 1051
Bme1390I	CCNGG	3 cut(s)	103, 532, 765
Bme18I	GGWCC	1 cut(s)	631
BmeRI	GACNNNNNGTC	2 cut(s)	44, 244
BmgT120I	GGNCC	3 cut(s)	194, 533, 631
BmiI	GGNNCC	1 cut(s)	131
BmrFI	CCNGG	3 cut(s)	103, 532, 765
BmrI	ACTGGG	2 cut(s)	252, 708
BmsI	GCATC	2 cut(s)	646, 981
BmuI	ACTGGG	2 cut(s)	252, 708
BpiI	GAAGAC	1 cut(s)	669
BpmI	CTGGAG	2 cut(s)	70, 753
Bpu10I	CCTNAGC	1 cut(s)	450
BpuEI	CTTGAG	2 cut(s)	756, 776
BpuMI	CCSGG	2 cut(s)	103, 532
BsaI	GGTCTC	1 cut(s)	163
BsaJI	CCNNGG	2 cut(s)	211, 471
BsaWI	WCCGGW	2 cut(s)	146, 154
BsaXI	ACNNNNNCTCC	2 cut(s)	539, 569
Bsc4I	CCNNNNNNNGG	3 cut(s)	41, 146, 331
Bse1I	ACTGG	5 cut(s)	247, 280, 387, 568, 703
Bse21I	CCTNAGG	1 cut(s)	301
Bse3DI	GCAATG	3 cut(s)	222, 639, 1035
BseBI	CCWGG	1 cut(s)	765
BseDI	CCNNGG	2 cut(s)	211, 471
BseGI	GGATG	1 cut(s)	10
BseLI	CCNNNNNNNGG	3 cut(s)	41, 146, 331
BseMI	GCAATG	3 cut(s)	222, 639, 1035
BseMII	CTCAG	3 cut(s)	108, 315, 441
BseNI	ACTGG	5 cut(s)	247, 280, 387, 568, 703
BseXI	GCAGC	3 cut(s)	430, 458, 1061
Bsh1236I	CGCG	1 cut(s)	37
BshFI	GGCC	6 cut(s)	196, 279, 299, 306, 476, 535
BsiHKAI	GWGCWC	3 cut(s)	72, 168, 772
BsiSI	CCGG	4 cut(s)	103, 147, 155, 531
BslFI	GGGAC	1 cut(s)	373
BslI	CCNNNNNNNGG	3 cut(s)	41, 146, 331
BsmAI	GTCTC	3 cut(s)	46, 163, 212
BsmFI	GGGAC	1 cut(s)	373
BsnI	GGCC	6 cut(s)	196, 279, 299, 306, 476, 535
Bso31I	GGTCTC	1 cut(s)	163
Bsp1286I	GDGCHC	3 cut(s)	72, 168, 772
Bsp143I	GATC	3 cut(s)	107, 751, 901
BspACI	CCGC	4 cut(s)	32, 35, 174, 998
BspANI	GGCC	6 cut(s)	196, 279, 299, 306, 476, 535
BspCNI	CTCAG	3 cut(s)	107, 314, 442
BspFNI	CGCG	1 cut(s)	37
BspHI	TCATGA	1 cut(s)	402
BspLI	GGNNCC	1 cut(s)	131
BspMAI	CTGCAG	1 cut(s)	448
BspPI	GGATC	1 cut(s)	746
BspTNI	GGTCTC	1 cut(s)	163
BsrDI	GCAATG	3 cut(s)	222, 639, 1035
BsrI	ACTGG	5 cut(s)	247, 280, 387, 568, 703
BssECI	CCNNGG	2 cut(s)	211, 471
BssMI	GATC	3 cut(s)	107, 751, 901
BssT1I	CCWWGG	1 cut(s)	471
Bst2UI	CCWGG	1 cut(s)	765
Bst4CI	ACNGT	3 cut(s)	461, 880, 952
Bst6I	CTCTTC	2 cut(s)	513, 1064
BstAPI	GCANNNNNTGC	1 cut(s)	1022
BstC8I	GCNNGC	4 cut(s)	308, 690, 936, 1027
BstDEI	CTNAG	3 cut(s)	94, 301, 450
BstDSI	CCRYGG	1 cut(s)	211
BstF5I	GGATG	1 cut(s)	10
BstFNI	CGCG	1 cut(s)	37
BstHHI	GCGC	2 cut(s)	80, 544
BstKTI	GATC	3 cut(s)	110, 754, 904
BstMAI	GTCTC	3 cut(s)	46, 163, 212
BstMBI	GATC	3 cut(s)	107, 751, 901
BstMWI	GCNNNNNNNGC	4 cut(s)	541, 995, 1022, 1046
BstNI	CCWGG	1 cut(s)	765
BstSCI	CCNGG	3 cut(s)	101, 530, 763
BstSFI	CTRYAG	1 cut(s)	444
BstUI	CGCG	1 cut(s)	37
BstV1I	GCAGC	3 cut(s)	430, 458, 1061
BstV2I	GAAGAC	1 cut(s)	669
Bsu36I	CCTNAGG	1 cut(s)	301
BsuRI	GGCC	6 cut(s)	196, 279, 299, 306, 476, 535
BtgI	CCRYGG	1 cut(s)	211
BtgZI	GCGATG	1 cut(s)	971
BtsCI	GGATG	1 cut(s)	10
BtsIMutI	CAGTG	5 cut(s)	240, 380, 466, 755, 948
Cac8I	GCNNGC	4 cut(s)	308, 690, 936, 1027
CaiI	CAGNNNCTG	1 cut(s)	680
CciI	TCATGA	1 cut(s)	402
CfoI	GCGC	2 cut(s)	80, 544
Cfr13I	GGNCC	3 cut(s)	194, 533, 631
CsiI	ACCWGGT	1 cut(s)	763
CviAII	CATG	2 cut(s)	403, 545
DdeI	CTNAG	3 cut(s)	94, 301, 450
DpnI	GATC	3 cut(s)	109, 753, 903
DpnII	GATC	3 cut(s)	107, 751, 901
DraIII	CACNNNGTG	1 cut(s)	574
DriI	GACNNNNNGTC	2 cut(s)	44, 244
EaeI	YGGCCR	2 cut(s)	277, 474
Eam1104I	CTCTTC	2 cut(s)	513, 1064
Eam1105I	GACNNNNNGTC	2 cut(s)	44, 244
EarI	CTCTTC	2 cut(s)	513, 1064
Ecl136II	GAGCTC	1 cut(s)	166
Eco130I	CCWWGG	1 cut(s)	471
Eco147I	AGGCCT	1 cut(s)	306
Eco24I	GRGCYC	1 cut(s)	168
Eco31I	GGTCTC	1 cut(s)	163
Eco47I	GGWCC	1 cut(s)	631
Eco53kI	GAGCTC	1 cut(s)	166
Eco57I	CTGAAG	2 cut(s)	702, 924
Eco81I	CCTNAGG	1 cut(s)	301
EcoICRI	GAGCTC	1 cut(s)	166
EcoRI	GAATTC	1 cut(s)	425
EcoRII	CCWGG	1 cut(s)	763
EcoT14I	CCWWGG	1 cut(s)	471
EcoT38I	GRGCYC	1 cut(s)	168
ErhI	CCWWGG	1 cut(s)	471
FaeI	CATG	2 cut(s)	406, 548
FaqI	GGGAC	1 cut(s)	373
FatI	CATG	2 cut(s)	402, 544
FbaI	TGATCA	1 cut(s)	901
FblI	GTMKAC	1 cut(s)	287
Fnu4HI	GCNGC	5 cut(s)	35, 444, 447, 999, 1050
FokI	GGATG	1 cut(s)	17
FriOI	GRGCYC	1 cut(s)	168
Fsp4HI	GCNGC	5 cut(s)	35, 444, 447, 999, 1050
FspBI	CTAG	3 cut(s)	671, 707, 740
GlaI	GCGC	2 cut(s)	79, 543
GluI	GCNGC	5 cut(s)	35, 444, 447, 999, 1050
GsuI	CTGGAG	2 cut(s)	70, 753
HaeIII	GGCC	6 cut(s)	196, 279, 299, 306, 476, 535
HapII	CCGG	4 cut(s)	103, 147, 155, 531
HhaI	GCGC	2 cut(s)	80, 544
Hin1II	CATG	2 cut(s)	406, 548
Hin6I	GCGC	2 cut(s)	78, 542
HinP1I	GCGC	2 cut(s)	78, 542
HincII	GTYRAC	2 cut(s)	391, 457
HindII	GTYRAC	2 cut(s)	391, 457
HinfI	GANTC	8 cut(s)	45, 60, 92, 361, 399, 523, 703, 980
HpaI	GTTAAC	2 cut(s)	391, 457
HpaII	CCGG	4 cut(s)	103, 147, 155, 531
HphI	GGTGA	6 cut(s)	314, 586, 832, 839, 896, 975
Hpy166II	GTNNAC	6 cut(s)	288, 334, 391, 457, 464, 631
Hpy188I	TCNGA	4 cut(s)	91, 163, 509, 751
Hpy188III	TCNNGA	6 cut(s)	49, 236, 264, 403, 422, 773
Hpy8I	GTNNAC	6 cut(s)	288, 334, 391, 457, 464, 631
HpyAV	CCTTC	3 cut(s)	571, 677, 919
HpyCH4III	ACNGT	3 cut(s)	461, 880, 952
HpyCH4IV	ACGT	1 cut(s)	257
HpyF10VI	GCNNNNNNNGC	4 cut(s)	541, 995, 1022, 1046
HpyF3I	CTNAG	3 cut(s)	94, 301, 450
HpySE526I	ACGT	1 cut(s)	257
Hsp92II	CATG	2 cut(s)	406, 548
HspAI	GCGC	2 cut(s)	78, 542
Ksp22I	TGATCA	1 cut(s)	901
KspAI	GTTAAC	2 cut(s)	391, 457
Kzo9I	GATC	3 cut(s)	107, 751, 901
LmnI	GCTCC	2 cut(s)	67, 171
Lsp1109I	GCAGC	3 cut(s)	430, 458, 1061
LweI	GCATC	2 cut(s)	646, 981
MabI	ACCWGGT	1 cut(s)	763
MaeI	CTAG	3 cut(s)	671, 707, 740
MaeII	ACGT	1 cut(s)	257
MaeIII	GTNAC	3 cut(s)	566, 820, 827
MalI	GATC	3 cut(s)	109, 753, 903
MboI	GATC	3 cut(s)	107, 751, 901
MboII	GAAGA	4 cut(s)	524, 530, 674, 1081
MfeI	CAATTG	4 cut(s)	369, 832, 1017, 1041
MhlI	GDGCHC	3 cut(s)	72, 168, 772
MlsI	TGGCCA	2 cut(s)	279, 476
MluNI	TGGCCA	2 cut(s)	279, 476
MlyI	GAGTC	6 cut(s)	54, 54, 86, 355, 393, 974
MmeI	TCCRAC	1 cut(s)	114
MnlI	CCTC	6 cut(s)	68, 310, 445, 565, 1039, 1092
Mox20I	TGGCCA	2 cut(s)	279, 476
MscI	TGGCCA	2 cut(s)	279, 476
MseI	TTAA	2 cut(s)	390, 456
MslI	CAYNNNNRTG	1 cut(s)	818
Msp20I	TGGCCA	2 cut(s)	279, 476
MspA1I	CMGCKG	1 cut(s)	449
MspI	CCGG	4 cut(s)	103, 147, 155, 531
MspR9I	CCNGG	3 cut(s)	103, 532, 765
MunI	CAATTG	4 cut(s)	369, 832, 1017, 1041
MvaI	CCWGG	1 cut(s)	765
MvnI	CGCG	1 cut(s)	37
MwoI	GCNNNNNNNGC	4 cut(s)	541, 995, 1022, 1046
NciI	CCSGG	2 cut(s)	103, 532
NdeII	GATC	3 cut(s)	107, 751, 901
NlaIII	CATG	2 cut(s)	406, 548
NlaIV	GGNNCC	1 cut(s)	131
NmuCI	GTSAC	3 cut(s)	566, 820, 827
PagI	TCATGA	1 cut(s)	402
PceI	AGGCCT	1 cut(s)	306
PfeI	GAWTC	2 cut(s)	523, 703
PflMI	CCANNNNNTGG	1 cut(s)	331
PkrI	GCNGC	5 cut(s)	36, 445, 448, 1000, 1051
PleI	GAGTC	6 cut(s)	53, 54, 86, 355, 393, 974
PpsI	GAGTC	6 cut(s)	53, 54, 86, 355, 393, 974
Psp124BI	GAGCTC	1 cut(s)	168
Psp6I	CCWGG	1 cut(s)	763
PspGI	CCWGG	1 cut(s)	763
PspN4I	GGNNCC	1 cut(s)	131
PspPI	GGNCC	3 cut(s)	194, 533, 631
PstI	CTGCAG	1 cut(s)	448
PstNI	CAGNNNCTG	1 cut(s)	680
PvuII	CAGCTG	1 cut(s)	449
RseI	CAYNNNNRTG	1 cut(s)	818
SacI	GAGCTC	1 cut(s)	168
SaqAI	TTAA	2 cut(s)	390, 456
SatI	GCNGC	5 cut(s)	35, 444, 447, 999, 1050
Sau3AI	GATC	3 cut(s)	107, 751, 901
Sau96I	GGNCC	3 cut(s)	194, 533, 631
SchI	GAGTC	6 cut(s)	54, 54, 86, 355, 393, 974
ScrFI	CCNGG	3 cut(s)	103, 532, 765
SduI	GDGCHC	3 cut(s)	72, 168, 772
SexAI	ACCWGGT	1 cut(s)	763
SfaNI	GCATC	2 cut(s)	646, 981
SfcI	CTRYAG	1 cut(s)	444
SinI	GGWCC	1 cut(s)	631
SmiMI	CAYNNNNRTG	1 cut(s)	818
SmlI	CTYRAG	2 cut(s)	771, 791
SmoI	CTYRAG	2 cut(s)	771, 791
SseBI	AGGCCT	1 cut(s)	306
SsiI	CCGC	4 cut(s)	32, 35, 174, 998
SspMI	CTAG	3 cut(s)	671, 707, 740
SstI	GAGCTC	1 cut(s)	168
StuI	AGGCCT	1 cut(s)	306
StyD4I	CCNGG	3 cut(s)	101, 530, 763
StyI	CCWWGG	1 cut(s)	471
TaaI	ACNGT	3 cut(s)	461, 880, 952
TaiI	ACGT	1 cut(s)	260
TaqI	TCGA	3 cut(s)	348, 421, 654
TauI	GCSGC	2 cut(s)	37, 1001
TfiI	GAWTC	2 cut(s)	523, 703
Tru1I	TTAA	2 cut(s)	390, 456
Tru9I	TTAA	2 cut(s)	390, 456
TscAI	CASTG	5 cut(s)	247, 387, 466, 762, 955
TseFI	GTSAC	3 cut(s)	566, 820, 827
TseI	GCWGC	3 cut(s)	443, 446, 1049
Tsp45I	GTSAC	3 cut(s)	566, 820, 827
TspDTI	ATGAA	7 cut(s)	35, 419, 447, 531, 815, 876, 1082
TspGWI	ACGGA	1 cut(s)	200
TspRI	CASTG	5 cut(s)	247, 387, 466, 762, 955
Van91I	CCANNNNNTGG	1 cut(s)	331
VpaK11BI	GGWCC	1 cut(s)	631
XapI	RAATTY	4 cut(s)	22, 425, 503, 847
XmiI	GTMKAC	1 cut(s)	287
XspI	CTAG	3 cut(s)	671, 707, 740

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.