Rh4AG140900

Basic leucine zipper and W2 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001412
Physical Location & Seq
Reverse (-)
35793323 .. 35801088
7766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG140900.1

Sequence Viewer

Length: 639 bp
ATGCATGAGCCACCTTTTGGGGCTCTTTCCAAGGCTATGACTGAAGATGAGCTCTACTATCTTAGGGCTCAGTTTACGCTCTTGGAACCCAAACATGGATACGTGTCCCTCGATAATTTCAGAACGGTTGTTTTCACTGGGGGCAGTACACAACCAGGAACAACAAAACCTGATAAGGGGGAGCGCCACCCTTACTCTGTACTAGAGTCTGAGCGTAGACGTGAAGTCAGTATACCATCTGTTATCTACATACAAAAGATTTTGAGGCGAAGGCCATTTCTGATAAAGAATCTCGAAAATGTCTTCCTTCAGTCATTGGAGCTTTTTGTGCAAAATGAAAGGAAGAAGCTAGCAATTTGCACAGCACTTGCATTCTCTCAAAAGCTATTAGGACTTACACTGGAAACGGTGTTCCAGCCAATGCTCATATTGGAAAAAGTTGATGTTGGTGTGCCCATGGCGAGACTGGTGTCGGGATGCTACCAAGAGTTGGTTACGGTGTCTGTTGGAAAAGAGCAGCATTGTTATCGACCCGCCACTGGAGTGTGTCGAGTCATAGCTGGTTTTGCCTATCTACTGCTGATGCATGGTTCTGTGCCTCAATCATGGAGGCATGTTCTCATGCTGCTTGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
W2

Protein Analysis

212

Amino Acids

24.04

Weight (kDa)

9.19

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_5MP1_2 PF25504 38 - 141 1.6e-19 5MP1/2 HEAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 17, 490
AccI GTMKAC 2 cut(s) 217, 232
AciI CCGC 1 cut(s) 534
AcuI CTGAAG 2 cut(s) 63, 293
AfaI GTAC 2 cut(s) 148, 201
AfiI CCNNNNNNNGG 5 cut(s) 17, 95, 176, 490, 539
AflIII ACRYGT 1 cut(s) 102
AhdI GACNNNNNGTC 1 cut(s) 224
AjiI CACGTC 1 cut(s) 221
AjnI CCWGG 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 542
AloI GAACNNNNNNTCC 2 cut(s) 395, 427
AluBI AGCT 5 cut(s) 52, 322, 349, 385, 560
AluI AGCT 5 cut(s) 52, 322, 349, 385, 560
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 1 cut(s) 457
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 517, 625
AspLEI GCGC 1 cut(s) 186
AsuNHI GCTAGC 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 456
BanII GRGCYC 3 cut(s) 25, 54, 70
BarI GAAGNNNNNNTAC 2 cut(s) 216, 248
BbsI GAAGAC 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 529, 612
BccI CCATC 1 cut(s) 244
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 1 cut(s) 156
BciVI GTATCC 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 203, 350
BfoI RGCGCY 1 cut(s) 187
BfuI GTATCC 1 cut(s) 92
BisI GCNGC 2 cut(s) 518, 626
BlsI GCNGC 2 cut(s) 519, 627
Bme1390I CCNGG 1 cut(s) 156
BmeRI GACNNNNNGTC 1 cut(s) 224
BmgBI CACGTC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 156
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 467, 573
BmtI GCTAGC 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 147
BoxI GACNNNNGTC 1 cut(s) 469
BpiI GAAGAC 1 cut(s) 295
BpmI CTGGAG 1 cut(s) 561
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 30, 456
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 95, 176, 490, 539
Bse1I ACTGG 4 cut(s) 142, 405, 471, 544
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 30, 456
BseGI GGATG 1 cut(s) 482
BseLI CCNNNNNNNGG 5 cut(s) 17, 95, 176, 490, 539
BseMII CTCAG 2 cut(s) 83, 201
BseNI ACTGG 4 cut(s) 142, 405, 471, 544
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 2 cut(s) 529, 612
BshFI GGCC 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 54
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 5 cut(s) 17, 95, 176, 490, 539
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 1 cut(s) 274
Bsp1286I GDGCHC 4 cut(s) 25, 54, 70, 456
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 1 cut(s) 534
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 2 cut(s) 82, 202
BspLI GGNNCC 1 cut(s) 87
BspOI GCTAGC 1 cut(s) 353
BsrI ACTGG 4 cut(s) 142, 405, 471, 544
BssECI CCNNGG 2 cut(s) 30, 456
BssNAI GTATAC 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 30, 456
Bst1107I GTATAC 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 3 cut(s) 127, 409, 499
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 351, 630
BstDEI CTNAG 3 cut(s) 62, 69, 210
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 482
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstMAI GTCTC 1 cut(s) 457
BstMWI GCNNNNNNNGC 2 cut(s) 328, 566
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 617
BstPAI GACNNNNGTC 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 529, 612
BstV2I GAAGAC 1 cut(s) 295
BstZ17I GTATAC 1 cut(s) 233
BsuI GTATCC 1 cut(s) 92
BsuRI GGCC 1 cut(s) 274
BtgI CCRYGG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 221
BtsCI GGATG 1 cut(s) 482
BtsIMutI CAGTG 3 cut(s) 135, 398, 537
Cac8I GCNNGC 2 cut(s) 351, 630
CfoI GCGC 1 cut(s) 186
Csp6I GTAC 2 cut(s) 147, 200
CviAII CATG 7 cut(s) 5, 95, 457, 587, 606, 614, 622
CviQI GTAC 2 cut(s) 147, 200
DdeI CTNAG 3 cut(s) 62, 69, 210
DriI GACNNNNNGTC 1 cut(s) 224
Eam1105I GACNNNNNGTC 1 cut(s) 224
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 30, 456
Eco24I GRGCYC 3 cut(s) 25, 54, 70
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 63, 293
EcoICRI GAGCTC 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 30, 456
EcoT22I ATGCAT 2 cut(s) 6, 588
EcoT38I GRGCYC 3 cut(s) 25, 54, 70
ErhI CCWWGG 2 cut(s) 30, 456
FaeI CATG 7 cut(s) 8, 98, 460, 590, 609, 617, 625
FaqI GGGAC 1 cut(s) 91
FatI CATG 7 cut(s) 4, 94, 456, 586, 605, 613, 621
FauI CCCGC 1 cut(s) 541
FblI GTMKAC 2 cut(s) 217, 232
Fnu4HI GCNGC 2 cut(s) 518, 626
FokI GGATG 1 cut(s) 489
FriOI GRGCYC 3 cut(s) 25, 54, 70
Fsp4HI GCNGC 2 cut(s) 518, 626
FspBI CTAG 2 cut(s) 203, 350
GlaI GCGC 1 cut(s) 185
GluI GCNGC 2 cut(s) 518, 626
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 1 cut(s) 274
HhaI GCGC 1 cut(s) 186
Hin1II CATG 7 cut(s) 8, 98, 460, 590, 609, 617, 625
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 3 cut(s) 206, 289, 552
Hpy166II GTNNAC 4 cut(s) 75, 149, 218, 233
Hpy188I TCNGA 3 cut(s) 122, 211, 282
Hpy188III TCNNGA 2 cut(s) 293, 474
Hpy8I GTNNAC 4 cut(s) 75, 149, 218, 233
HpyAV CCTTC 2 cut(s) 264, 317
HpyCH4III ACNGT 3 cut(s) 127, 409, 499
HpyCH4IV ACGT 2 cut(s) 102, 220
HpyCH4V TGCA 5 cut(s) 4, 331, 360, 371, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 328, 566
HpyF3I CTNAG 3 cut(s) 62, 69, 210
HpySE526I ACGT 2 cut(s) 102, 220
Hsp92II CATG 7 cut(s) 8, 98, 460, 590, 609, 617, 625
HspAI GCGC 1 cut(s) 184
LmnI GCTCC 3 cut(s) 181, 319, 637
LpnPI CCDG 9 cut(s) 123, 141, 168, 183, 386, 428, 452, 525, 546
Lsp1109I GCAGC 2 cut(s) 529, 612
LweI GCATC 2 cut(s) 467, 573
MaeI CTAG 2 cut(s) 203, 350
MaeII ACGT 2 cut(s) 102, 220
MaeIII GTNAC 1 cut(s) 493
MboII GAAGA 3 cut(s) 56, 295, 355
MhlI GDGCHC 4 cut(s) 25, 54, 70, 456
MluCI AATT 2 cut(s) 115, 354
MlyI GAGTC 2 cut(s) 215, 561
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 4 cut(s) 119, 258, 603, 609
Mph1103I ATGCAT 2 cut(s) 6, 588
MslI CAYNNNNRTG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 328, 566
NcoI CCATGG 1 cut(s) 456
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 7 cut(s) 8, 98, 460, 590, 609, 617, 625
NlaIV GGNNCC 1 cut(s) 87
NsiI ATGCAT 2 cut(s) 6, 588
NspI RCATGY 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 542
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 289
PflMI CCANNNNNTGG 2 cut(s) 17, 490
PkrI GCNGC 2 cut(s) 519, 627
PleI GAGTC 2 cut(s) 214, 560
PpsI GAGTC 2 cut(s) 214, 560
Ppu21I YACGTR 1 cut(s) 103
PshAI GACNNNNGTC 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 87
RsaI GTAC 2 cut(s) 148, 201
RsaNI GTAC 2 cut(s) 147, 200
RseI CAYNNNNRTG 1 cut(s) 542
SacI GAGCTC 1 cut(s) 54
SatI GCNGC 2 cut(s) 518, 626
SchI GAGTC 2 cut(s) 215, 561
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 4 cut(s) 25, 54, 70, 456
SetI ASST 9 cut(s) 16, 54, 105, 172, 223, 324, 351, 387, 562
SfaNI GCATC 2 cut(s) 467, 573
SmiMI CAYNNNNRTG 1 cut(s) 542
Sse9I AATT 2 cut(s) 115, 354
SsiI CCGC 1 cut(s) 534
SspMI CTAG 2 cut(s) 203, 350
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 2 cut(s) 30, 456
TaaI ACNGT 3 cut(s) 127, 409, 499
TaiI ACGT 2 cut(s) 105, 223
TaqI TCGA 4 cut(s) 111, 294, 529, 550
TasI AATT 2 cut(s) 115, 354
TatI WGTACW 2 cut(s) 146, 199
TfiI GAWTC 1 cut(s) 289
TscAI CASTG 3 cut(s) 142, 405, 544
TseI GCWGC 2 cut(s) 517, 625
TspDTI ATGAA 1 cut(s) 351
TspRI CASTG 3 cut(s) 142, 405, 544
Van91I CCANNNNNTGG 2 cut(s) 17, 490
XceI RCATGY 1 cut(s) 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmiI GTMKAC 2 cut(s) 217, 232
XspI CTAG 2 cut(s) 203, 350
Zsp2I ATGCAT 2 cut(s) 6, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.