Rh4CG147700

Basic leucine zipper and W2 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001412
Physical Location & Seq
Reverse (-)
32520589 .. 32528787
8199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG147700.1

Sequence Viewer

Length: 750 bp
ATGACTGCTGCTCAATCTTTAACCCACCCATGGCTAAGGGATGAAAGACAAGCTGTGCCTTTAGATTTAGTAATCCACAAGTTGGTTAAGTCATATGTTCGTGCCACACCTTTCAGGCGTGCAGCAATGAAGGCTCTTTCCAAGGCTATGACAGAAGATGAGCTCTACTATCTTAGGGCTCAGTTTATGCTCTTGGAACCCAAACATGGATACGTGTCCCTCGATAATTTCAGAACGGTTGTTTTCACTGGGGGCAGTACACAACCAGGAACAACAAAACCTGATAAGGGGGAGCGCCACCCTTACTCTGTACTAGAGTCTGAGCGTAGACGTGAAGTCAGTATACCATCTGTTATCTACATACAAAAGATTTTGAGGCGAAGGCCATTTCTGATAAAGAATCTCGAAAATGTCTTCCTTCAGTCATTGGAGCTTTTTGTGGAAAATGAAAGGAAGAAGCTAGCAATTTGCACAGCACTTGCATTCTCTCAAAAGCTATTAGGACTTACACTGGAAACGGTGTTCCAGCCAATGCTCATATTGGAAAAAGTTGATGTTGGTGTGCCCATGGCGAGACTGGTGTCGGGATGCTACCAAGAGTTGGTTACGGTGTCTGTTGGAAAAGAGCAGCATTGTTATCGACCCGCCACTGGAGTGTGTCGAGTCATAGCTGGTTTTGCCTATCTACTGCTGATGCATGGTTCTGTGCCTCAATCATGGAGGCATGTTCTCATGCTGCTTGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
W2

Protein Analysis

249

Amino Acids

28.36

Weight (kDa)

9.61

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_5MP1_2 PF25504 74 - 178 1e-19 5MP1/2 HEAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 82, 601
AccI GTMKAC 2 cut(s) 328, 343
AciI CCGC 1 cut(s) 645
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 2 cut(s) 259, 312
AfiI CCNNNNNNNGG 6 cut(s) 30, 82, 206, 287, 601, 650
AflIII ACRYGT 1 cut(s) 213
AhdI GACNNNNNGTC 1 cut(s) 335
AjiI CACGTC 1 cut(s) 332
AjnI CCWGG 1 cut(s) 265
AleI CACNNNNGTG 1 cut(s) 653
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 6 cut(s) 53, 163, 433, 460, 496, 671
AluI AGCT 6 cut(s) 53, 163, 433, 460, 496, 671
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 568
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 4 cut(s) 8, 122, 628, 736
AspLEI GCGC 1 cut(s) 297
AsuNHI GCTAGC 1 cut(s) 460
BaeGI GKGCMC 1 cut(s) 567
BanII GRGCYC 2 cut(s) 165, 181
BarI GAAGNNNNNNTAC 2 cut(s) 327, 359
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 3 cut(s) 134, 640, 723
BccI CCATC 1 cut(s) 355
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 1 cut(s) 267
BciVI GTATCC 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 2 cut(s) 314, 461
BfoI RGCGCY 1 cut(s) 298
BfuI GTATCC 1 cut(s) 203
BisI GCNGC 4 cut(s) 9, 123, 629, 737
BlsI GCNGC 4 cut(s) 10, 124, 630, 738
Bme1390I CCNGG 1 cut(s) 267
BmeRI GACNNNNNGTC 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 267
BmrI ACTGGG 1 cut(s) 258
BmsI GCATC 2 cut(s) 578, 684
BmtI GCTAGC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 258
BoxI GACNNNNGTC 1 cut(s) 580
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 35
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 29, 141, 567
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 82, 206, 287, 601, 650
Bse1I ACTGG 4 cut(s) 253, 516, 582, 655
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 29, 141, 567
BseGI GGATG 2 cut(s) 46, 593
BseLI CCNNNNNNNGG 6 cut(s) 30, 82, 206, 287, 601, 650
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 2 cut(s) 194, 312
BseNI ACTGG 4 cut(s) 253, 516, 582, 655
BseSI GKGCMC 1 cut(s) 567
BseXI GCAGC 3 cut(s) 134, 640, 723
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 165
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 6 cut(s) 30, 82, 206, 287, 601, 650
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 482
BsnI GGCC 1 cut(s) 385
Bsp1286I GDGCHC 3 cut(s) 165, 181, 567
Bsp19I CCATGG 2 cut(s) 29, 567
BspACI CCGC 1 cut(s) 645
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 2 cut(s) 193, 313
BspLI GGNNCC 1 cut(s) 198
BspOI GCTAGC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 4 cut(s) 253, 516, 582, 655
BssECI CCNNGG 3 cut(s) 29, 141, 567
BssNAI GTATAC 1 cut(s) 344
BssT1I CCWWGG 3 cut(s) 29, 141, 567
Bst1107I GTATAC 1 cut(s) 344
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 238, 520, 610
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 3 cut(s) 120, 462, 741
BstDEI CTNAG 4 cut(s) 35, 173, 180, 321
BstDSI CCRYGG 2 cut(s) 29, 567
BstF5I GGATG 2 cut(s) 46, 593
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 1 cut(s) 297
BstMAI GTCTC 1 cut(s) 568
BstMWI GCNNNNNNNGC 2 cut(s) 131, 677
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 1 cut(s) 728
BstPAI GACNNNNGTC 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 134, 640, 723
BstV2I GAAGAC 1 cut(s) 406
BstZ17I GTATAC 1 cut(s) 344
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 1 cut(s) 385
BtgI CCRYGG 2 cut(s) 29, 567
BtrI CACGTC 1 cut(s) 332
BtsCI GGATG 2 cut(s) 46, 593
BtsIMutI CAGTG 3 cut(s) 246, 509, 648
Cac8I GCNNGC 3 cut(s) 120, 462, 741
CfoI GCGC 1 cut(s) 297
Csp6I GTAC 2 cut(s) 258, 311
CviAII CATG 7 cut(s) 30, 206, 568, 698, 717, 725, 733
CviQI GTAC 2 cut(s) 258, 311
DdeI CTNAG 4 cut(s) 35, 173, 180, 321
DriI GACNNNNNGTC 1 cut(s) 335
Eam1105I GACNNNNNGTC 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 163
Eco130I CCWWGG 3 cut(s) 29, 141, 567
Eco24I GRGCYC 2 cut(s) 165, 181
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 3 cut(s) 29, 141, 567
EcoT22I ATGCAT 1 cut(s) 699
EcoT38I GRGCYC 2 cut(s) 165, 181
ErhI CCWWGG 3 cut(s) 29, 141, 567
FaeI CATG 7 cut(s) 33, 209, 571, 701, 720, 728, 736
FaqI GGGAC 1 cut(s) 202
FatI CATG 7 cut(s) 29, 205, 567, 697, 716, 724, 732
FauI CCCGC 1 cut(s) 652
FauNDI CATATG 1 cut(s) 94
FblI GTMKAC 2 cut(s) 328, 343
Fnu4HI GCNGC 4 cut(s) 9, 123, 629, 737
FokI GGATG 2 cut(s) 53, 600
FriOI GRGCYC 2 cut(s) 165, 181
Fsp4HI GCNGC 4 cut(s) 9, 123, 629, 737
FspBI CTAG 2 cut(s) 314, 461
GlaI GCGC 1 cut(s) 296
GluI GCNGC 4 cut(s) 9, 123, 629, 737
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 1 cut(s) 385
HhaI GCGC 1 cut(s) 297
Hin1II CATG 7 cut(s) 33, 209, 571, 701, 720, 728, 736
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HinfI GANTC 3 cut(s) 317, 400, 663
Hpy166II GTNNAC 3 cut(s) 260, 329, 344
Hpy188I TCNGA 3 cut(s) 233, 322, 393
Hpy188III TCNNGA 2 cut(s) 404, 585
Hpy8I GTNNAC 3 cut(s) 260, 329, 344
HpyAV CCTTC 3 cut(s) 124, 375, 428
HpyCH4III ACNGT 3 cut(s) 238, 520, 610
HpyCH4IV ACGT 2 cut(s) 213, 331
HpyCH4V TGCA 4 cut(s) 122, 471, 482, 697
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 677
HpyF3I CTNAG 4 cut(s) 35, 173, 180, 321
HpySE526I ACGT 2 cut(s) 213, 331
Hsp92II CATG 7 cut(s) 33, 209, 571, 701, 720, 728, 736
HspAI GCGC 1 cut(s) 295
LmnI GCTCC 3 cut(s) 292, 430, 748
Lsp1109I GCAGC 3 cut(s) 134, 640, 723
LweI GCATC 2 cut(s) 578, 684
MaeI CTAG 2 cut(s) 314, 461
MaeII ACGT 2 cut(s) 213, 331
MaeIII GTNAC 1 cut(s) 604
MboII GAAGA 3 cut(s) 167, 406, 466
MhlI GDGCHC 3 cut(s) 165, 181, 567
MluCI AATT 2 cut(s) 226, 465
MlyI GAGTC 2 cut(s) 326, 672
MmeI TCCRAC 1 cut(s) 598
MnlI CCTC 4 cut(s) 230, 369, 714, 720
Mph1103I ATGCAT 1 cut(s) 699
MseI TTAA 2 cut(s) 20, 87
MslI CAYNNNNRTG 1 cut(s) 653
MspR9I CCNGG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 482
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 131, 677
NcoI CCATGG 2 cut(s) 29, 567
NdeI CATATG 1 cut(s) 94
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 7 cut(s) 33, 209, 571, 701, 720, 728, 736
NlaIV GGNNCC 1 cut(s) 198
NsiI ATGCAT 1 cut(s) 699
NspI RCATGY 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 653
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 400
PflMI CCANNNNNTGG 2 cut(s) 82, 601
PkrI GCNGC 4 cut(s) 10, 124, 630, 738
PleI GAGTC 2 cut(s) 325, 671
PpsI GAGTC 2 cut(s) 325, 671
Ppu21I YACGTR 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 580
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 198
RsaI GTAC 2 cut(s) 259, 312
RsaNI GTAC 2 cut(s) 258, 311
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 165
SaqAI TTAA 2 cut(s) 20, 87
SatI GCNGC 4 cut(s) 9, 123, 629, 737
SchI GAGTC 2 cut(s) 326, 672
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 3 cut(s) 165, 181, 567
SfaNI GCATC 2 cut(s) 578, 684
SmiMI CAYNNNNRTG 1 cut(s) 653
Sse9I AATT 2 cut(s) 226, 465
SsiI CCGC 1 cut(s) 645
SspMI CTAG 2 cut(s) 314, 461
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 3 cut(s) 29, 141, 567
TaaI ACNGT 3 cut(s) 238, 520, 610
TaiI ACGT 2 cut(s) 216, 334
TaqI TCGA 4 cut(s) 222, 405, 640, 661
TasI AATT 2 cut(s) 226, 465
TatI WGTACW 2 cut(s) 257, 310
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 2 cut(s) 20, 87
Tru9I TTAA 2 cut(s) 20, 87
TscAI CASTG 3 cut(s) 253, 516, 655
TseI GCWGC 4 cut(s) 8, 122, 628, 736
TspDTI ATGAA 3 cut(s) 57, 143, 462
TspRI CASTG 3 cut(s) 253, 516, 655
Van91I CCANNNNNTGG 2 cut(s) 82, 601
XceI RCATGY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 2 cut(s) 328, 343
XspI CTAG 2 cut(s) 314, 461
Zsp2I ATGCAT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.