Rh5DG211500

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002057
Physical Location & Seq
Reverse (-)
23702456 .. 23714962
12507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG211500.1

Sequence Viewer

Length: 801 bp
ATGACTTGCACATTCTTCTTCCCTAGGCTTCTTGTTCATTCTATCTTGCTGGTTATCTATGCAAGCTTTGCTTTCGGAGCTAAGCGATTATCAGAAGATGAAGTACAAGCTTTAAAGATCATGGGAAAGACACTGGGAAAGGACTGGGATTTCACTGCAGATCCTTGTAGCGAAGCATCTGGATGGATTACTCCCTCAACAAATAGCTCATTTGCGAACAACGTCACCTGCGGCAATTGTAGTACTGCTGGCGATGATCAGATCTGCCATGTTACAAACATACTTTTGAAATCTCAGAATTTGCCGGGAAAACTCCCGCCAGAGTTGATCAGGCTCCCCTATCTCCAGGAAATTGATCTCTCCCGCAACTACCTGAATGGCACCATCCCTCCAGAATGGGGTGCCCTGCCACTACTTAACATGAGAATTACTGACTTGAATGGCCCTGAATCATCATTTCCTCCACTTGAGAATATGAAAAAACCAAAGATACTGATGTTGCGGAATTGCAATATTAGTGGACAGCTACCTGCATATCTTGGGGGAATGACAACTTTGAAAACTTTAGACCTCAGCTTTAACAAGCTAACCGGAGAAATTCCAAGCAGCTTTTCTGGTCTAGCAAAAATTGATTACATGTTCTTGACTGGAAATATGCTCACTGGATCTGTGCCAGATTGGATGAATAGAGGAGGCAACATTGATCTTTCATACAACGACTTCACCACTAAGTCAGAGGCTGTCAGTTGTGAAGGCGGCTTGAACTTGTTTGCAAGCTCTTCCGGGGGCAACACTACGTAA

Protein Analysis

266

Amino Acids

28.97

Weight (kDa)

5.24

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 164 - 220 6.6e-09 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc36I ACCTGC 2 cut(s) 236, 538
AccB1I GGYRCC 2 cut(s) 380, 401
AciI CCGC 5 cut(s) 231, 317, 364, 502, 756
AclWI GGATC 2 cut(s) 155, 673
AcsI RAATTY 2 cut(s) 298, 597
AfaI GTAC 2 cut(s) 105, 244
AfiI CCNNNNNNNGG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 4 cut(s) 289, 439, 559, 763
AjnI CCWGG 1 cut(s) 345
AjuI GAANNNNNNNTTGG 2 cut(s) 595, 627
AluBI AGCT 9 cut(s) 66, 80, 110, 207, 526, 576, 586, 609, 777
AluI AGCT 9 cut(s) 66, 80, 110, 207, 526, 576, 586, 609, 777
AlwI GGATC 2 cut(s) 155, 673
AlwNI CAGNNNCTG 1 cut(s) 740
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 1 cut(s) 606
ApoI RAATTY 2 cut(s) 298, 597
AspA2I CCTAGG 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 443
AsuC2I CCSGG 2 cut(s) 306, 784
AsuHPI GGTGA 2 cut(s) 217, 715
AvrII CCTAGG 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 406
BaeI ACNNNNGTAYC 2 cut(s) 482, 515
BanI GGYRCC 2 cut(s) 380, 401
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BbvCI CCTCAGC 1 cut(s) 572
BbvI GCAGC 1 cut(s) 618
BccI CCATC 2 cut(s) 177, 392
BciT130I CCWGG 1 cut(s) 347
BclI TGATCA 2 cut(s) 256, 327
BcnI CCSGG 2 cut(s) 306, 784
BfaI CTAG 2 cut(s) 24, 620
BfmI CTRYAG 1 cut(s) 156
BfuAI ACCTGC 2 cut(s) 236, 538
BglII AGATCT 1 cut(s) 261
BisI GCNGC 3 cut(s) 232, 607, 757
BlnI CCTAGG 1 cut(s) 23
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 3 cut(s) 233, 608, 758
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 3 cut(s) 306, 347, 784
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 3 cut(s) 335, 382, 403
BmrFI CCNGG 3 cut(s) 306, 347, 784
BmrI ACTGGG 2 cut(s) 143, 154
BmsI GCATC 1 cut(s) 185
BmuI ACTGGG 2 cut(s) 143, 154
BpmI CTGGAG 2 cut(s) 329, 375
Bpu10I CCTNAGC 1 cut(s) 572
Bpu1102I GCTNAGC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 2 cut(s) 306, 784
BsaAI YACGTR 1 cut(s) 798
BsaJI CCNNGG 2 cut(s) 23, 783
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 4 cut(s) 138, 149, 652, 667
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 2 cut(s) 23, 783
BseGI GGATG 3 cut(s) 188, 384, 687
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 2 cut(s) 308, 586
BseNI ACTGG 4 cut(s) 138, 149, 652, 667
BseRI GAGGAG 1 cut(s) 705
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 1 cut(s) 618
BshFI GGCC 1 cut(s) 444
BshNI GGYRCC 2 cut(s) 380, 401
BsiSI CCGG 3 cut(s) 305, 591, 783
BslI CCNNNNNNNGG 1 cut(s) 398
BsnI GGCC 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
Bsp1720I GCTNAGC 1 cut(s) 81
BspACI CCGC 5 cut(s) 231, 317, 364, 502, 756
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 307, 585
BspLI GGNNCC 3 cut(s) 335, 382, 403
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 2 cut(s) 236, 538
BspPI GGATC 2 cut(s) 155, 673
BspQI GCTCTTC 1 cut(s) 784
BspT107I GGYRCC 2 cut(s) 380, 401
BsrI ACTGG 4 cut(s) 138, 149, 652, 667
BssECI CCNNGG 2 cut(s) 23, 783
BssMI GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 784
BstAPI GCANNNNNTGC 1 cut(s) 68
BstBAI YACGTR 1 cut(s) 798
BstC8I GCNNGC 3 cut(s) 64, 250, 775
BstDEI CTNAG 4 cut(s) 81, 294, 572, 729
BstF5I GGATG 3 cut(s) 188, 384, 687
BstKTI GATC 8 cut(s) 120, 163, 259, 264, 330, 358, 668, 706
BstMBI GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
BstMWI GCNNNNNNNGC 2 cut(s) 68, 77
BstNI CCWGG 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 3 cut(s) 304, 345, 782
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 406
BstSNI TACGTA 1 cut(s) 798
BstV1I GCAGC 1 cut(s) 618
BstX2I RGATCY 3 cut(s) 160, 261, 665
BstYI RGATCY 3 cut(s) 160, 261, 665
BsuRI GGCC 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 267
BtsCI GGATG 3 cut(s) 188, 384, 687
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 3 cut(s) 131, 153, 660
BveI ACCTGC 2 cut(s) 236, 538
Cac8I GCNNGC 3 cut(s) 64, 250, 775
CaiI CAGNNNCTG 1 cut(s) 740
Cfr13I GGNCC 1 cut(s) 443
Csp6I GTAC 2 cut(s) 104, 243
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CviAII CATG 4 cut(s) 121, 269, 421, 637
CviQI GTAC 2 cut(s) 104, 243
DdeI CTNAG 4 cut(s) 81, 294, 572, 729
DpnI GATC 8 cut(s) 119, 162, 258, 263, 329, 357, 667, 705
DpnII GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
DraI TTTAAA 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 784
EarI CTCTTC 1 cut(s) 784
Eco105I TACGTA 1 cut(s) 798
Eco130I CCWWGG 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 345
EcoT14I CCWWGG 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 4 cut(s) 124, 272, 424, 640
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FatI CATG 4 cut(s) 120, 268, 420, 636
FauI CCCGC 2 cut(s) 324, 371
FbaI TGATCA 2 cut(s) 256, 327
Fnu4HI GCNGC 3 cut(s) 232, 607, 757
FokI GGATG 3 cut(s) 195, 371, 694
Fsp4HI GCNGC 3 cut(s) 232, 607, 757
FspBI CTAG 2 cut(s) 24, 620
GluI GCNGC 3 cut(s) 232, 607, 757
GsuI CTGGAG 2 cut(s) 329, 375
HaeIII GGCC 1 cut(s) 444
HapII CCGG 3 cut(s) 305, 591, 783
Hin1II CATG 4 cut(s) 124, 272, 424, 640
HindIII AAGCTT 2 cut(s) 64, 108
HinfI GANTC 1 cut(s) 449
HpaII CCGG 3 cut(s) 305, 591, 783
HphI GGTGA 2 cut(s) 217, 715
Hpy166II GTNNAC 1 cut(s) 521
Hpy188I TCNGA 5 cut(s) 77, 94, 261, 297, 736
Hpy188III TCNNGA 3 cut(s) 180, 392, 643
Hpy8I GTNNAC 1 cut(s) 521
HpyAV CCTTC 1 cut(s) 746
HpyCH4IV ACGT 2 cut(s) 222, 797
HpyCH4V TGCA 6 cut(s) 9, 62, 158, 510, 533, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 77
HpyF3I CTNAG 4 cut(s) 81, 294, 572, 729
HpySE526I ACGT 2 cut(s) 222, 797
Hsp92II CATG 4 cut(s) 124, 272, 424, 640
Ksp22I TGATCA 2 cut(s) 256, 327
Kzo9I GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
LguI GCTCTTC 1 cut(s) 784
LmnI GCTCC 2 cut(s) 77, 339
Lsp1109I GCAGC 1 cut(s) 618
LweI GCATC 1 cut(s) 185
MaeI CTAG 2 cut(s) 24, 620
MaeII ACGT 2 cut(s) 222, 797
MaeIII GTNAC 2 cut(s) 223, 271
MalI GATC 8 cut(s) 119, 162, 258, 263, 329, 357, 667, 705
MboI GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
MboII GAAGA 4 cut(s) 7, 10, 107, 771
MfeI CAATTG 1 cut(s) 235
MflI RGATCY 3 cut(s) 160, 261, 665
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 7 cut(s) 235, 298, 351, 426, 505, 597, 627
MnlI CCTC 7 cut(s) 205, 399, 471, 581, 683, 686, 730
MseI TTAA 3 cut(s) 113, 417, 579
MslI CAYNNNNRTG 1 cut(s) 181
MspI CCGG 3 cut(s) 305, 591, 783
MspR9I CCNGG 3 cut(s) 306, 347, 784
MunI CAATTG 1 cut(s) 235
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 68, 77
NciI CCSGG 2 cut(s) 306, 784
NdeII GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
NlaIII CATG 4 cut(s) 124, 272, 424, 640
NlaIV GGNNCC 3 cut(s) 335, 382, 403
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 640
PaqCI CACCTGC 1 cut(s) 236
PciI ACATGT 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 784
PfeI GAWTC 1 cut(s) 449
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 3 cut(s) 233, 608, 758
Ppu21I YACGTR 1 cut(s) 798
PscI ACATGT 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 335, 382, 403
PspPI GGNCC 1 cut(s) 443
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 3 cut(s) 160, 261, 665
RsaI GTAC 2 cut(s) 105, 244
RsaNI GTAC 2 cut(s) 104, 243
RseI CAYNNNNRTG 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 784
SaqAI TTAA 3 cut(s) 113, 417, 579
SatI GCNGC 3 cut(s) 232, 607, 757
Sau3AI GATC 8 cut(s) 117, 160, 256, 261, 327, 355, 665, 703
Sau96I GGNCC 1 cut(s) 443
ScaI AGTACT 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 306, 347, 784
SduI GDGCHC 1 cut(s) 406
SfaNI GCATC 1 cut(s) 185
SfcI CTRYAG 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 181
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SnaBI TACGTA 1 cut(s) 798
Sse9I AATT 7 cut(s) 235, 298, 351, 426, 505, 597, 627
SsiI CCGC 5 cut(s) 231, 317, 364, 502, 756
SspI AATATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 24, 620
StyD4I CCNGG 3 cut(s) 304, 345, 782
StyI CCWWGG 1 cut(s) 23
TaiI ACGT 2 cut(s) 225, 800
TasI AATT 7 cut(s) 235, 298, 351, 426, 505, 597, 627
TatI WGTACW 2 cut(s) 103, 242
TauI GCSGC 2 cut(s) 234, 759
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 113, 417, 579
Tru9I TTAA 3 cut(s) 113, 417, 579
TscAI CASTG 3 cut(s) 138, 160, 667
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 606
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 5 cut(s) 26, 114, 491, 698, 699
TspRI CASTG 3 cut(s) 138, 160, 667
XapI RAATTY 2 cut(s) 298, 597
XceI RCATGY 1 cut(s) 640
XmaJI CCTAGG 1 cut(s) 23
XspI CTAG 2 cut(s) 24, 620
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.