Rw1G035930
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001340
Physical Location & Seq
Forward (+)
63746363 .. 63747133
771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035930.1

Sequence Viewer

Length: 705 bp
ATGTTTTCAGGTTGTGGCCTCATTAGATATATTGATGGATCATTCCCTCGTCCACCACAATACATAGTTATAGAAGAAGGTGGGGTTACGTCTGATATGACAAAGGAGTACATGGATTGGGTGCAGAATGATTCTGCTGTTATGTCTATTCTTGCAGCTACTCTGTCTAGTGATGCTCTCTCCTTCATTGTTGGAAGTAAGATCTCTTGGGAAATTTGGTTGTATAACATAATGAATCTCAAGGCCGCCTTATACAGCATCCAAAAAGTCAAGTCAATTTGTGATCAGCTTGCTACGATGGGAGTTTATATGGCCAATGAAGACATAGTAGACTCGGTTCTTCATGGTTTACCCTCAGAGTTTGCTACCATTAAAACTATAATAAGAATCAAAACACTAAACTCCTCTGTTTCAATGGAAGAATTAAGATCTCTTTTGTTGATCGCTGAAAGTGAGATTAAACAGACTATGAAGTCCATGCTTGAGAGGTTCAAGACTCAAATGATGGCACATGGTGATAGTTTCAATAGGTATGCTCCTGCACCAACTCATGTTACAAATGCCAAATATGATGGTGGAATTCAATACAACTTTGGAGCATTTGATATGGTATTACTCAGCAAAATGGAATATTTCAGCAAGGACACAACTTTCGGCAAATTGGAGGTCTCATGCAACAAAATGGTAACTTTTCTCCAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

234

Amino Acids

26.3

Weight (kDa)

5.44

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 87 - 153 6.7e-06 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 472
AccI GTMKAC 1 cut(s) 330
AciI CCGC 1 cut(s) 246
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 2 cut(s) 213, 579
AdeI CACNNNGTG 1 cut(s) 515
AfaI GTAC 1 cut(s) 110
AgsI TTSAA 4 cut(s) 414, 493, 526, 584
AluBI AGCT 2 cut(s) 158, 289
AluI AGCT 2 cut(s) 158, 289
Alw26I GTCTC 1 cut(s) 673
AlwI GGATC 1 cut(s) 46
AoxI GGCC 3 cut(s) 16, 243, 312
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 2 cut(s) 213, 579
ArsI GACNNNNNNTTYG 4 cut(s) 635, 651, 667, 683
AsuHPI GGTGA 1 cut(s) 527
BalI TGGCCA 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 167
BccI CCATC 4 cut(s) 29, 292, 499, 566
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 673
BfaI CTAG 1 cut(s) 168
BglII AGATCT 2 cut(s) 201, 428
BisI GCNGC 2 cut(s) 156, 246
BlsI GCNGC 2 cut(s) 157, 247
BmsI GCATC 2 cut(s) 163, 267
BpiI GAAGAC 1 cut(s) 327
BpuEI CTTGAG 2 cut(s) 224, 503
BsaI GGTCTC 1 cut(s) 673
BseGI GGATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 369, 631
BseRI GAGGAG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 167
BsgI GTGCAG 2 cut(s) 143, 525
BshFI GGCC 3 cut(s) 18, 245, 314
BsmAI GTCTC 1 cut(s) 673
BsnI GGCC 3 cut(s) 18, 245, 314
Bso31I GGTCTC 1 cut(s) 673
Bsp143I GATC 5 cut(s) 38, 201, 283, 428, 441
BspACI CCGC 1 cut(s) 246
BspANI GGCC 3 cut(s) 18, 245, 314
BspCNI CTCAG 2 cut(s) 368, 630
BspPI GGATC 1 cut(s) 46
BspTNI GGTCTC 1 cut(s) 673
BssMI GATC 5 cut(s) 38, 201, 283, 428, 441
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 2 cut(s) 355, 617
BstF5I GGATG 1 cut(s) 258
BstKTI GATC 5 cut(s) 41, 204, 286, 431, 444
BstMAI GTCTC 1 cut(s) 673
BstMBI GATC 5 cut(s) 38, 201, 283, 428, 441
BstV1I GCAGC 1 cut(s) 167
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 2 cut(s) 201, 428
BstYI RGATCY 2 cut(s) 201, 428
BsuRI GGCC 3 cut(s) 18, 245, 314
BtsCI GGATG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 109
CviAII CATG 6 cut(s) 112, 344, 478, 512, 551, 672
CviJI RGCY 5 cut(s) 18, 158, 245, 289, 314
CviKI_1 RGCY 5 cut(s) 18, 158, 245, 289, 314
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 2 cut(s) 355, 617
DpnI GATC 5 cut(s) 40, 203, 285, 430, 443
DpnII GATC 5 cut(s) 38, 201, 283, 428, 441
DraIII CACNNNGTG 1 cut(s) 515
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 579
FaeI CATG 6 cut(s) 115, 347, 481, 515, 554, 675
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 6 cut(s) 111, 343, 477, 511, 550, 671
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 2 cut(s) 156, 246
FokI GGATG 1 cut(s) 245
Fsp4HI GCNGC 2 cut(s) 156, 246
FspBI CTAG 1 cut(s) 168
GluI GCNGC 2 cut(s) 156, 246
HaeIII GGCC 3 cut(s) 18, 245, 314
Hin1II CATG 6 cut(s) 115, 347, 481, 515, 554, 675
HinfI GANTC 5 cut(s) 131, 235, 332, 387, 496
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 3 cut(s) 53, 331, 350
Hpy188I TCNGA 2 cut(s) 94, 358
Hpy188III TCNNGA 1 cut(s) 493
Hpy8I GTNNAC 3 cut(s) 53, 331, 350
HpyAV CCTTC 2 cut(s) 71, 193
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 4 cut(s) 124, 155, 542, 675
HpyF3I CTNAG 2 cut(s) 355, 617
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 6 cut(s) 115, 347, 481, 515, 554, 675
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 5 cut(s) 38, 201, 283, 428, 441
LmnI GCTCC 2 cut(s) 541, 596
LpnPI CCDG 1 cut(s) 552
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 2 cut(s) 163, 267
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 3 cut(s) 85, 553, 685
MalI GATC 5 cut(s) 40, 203, 285, 430, 443
MboI GATC 5 cut(s) 38, 201, 283, 428, 441
MboII GAAGA 4 cut(s) 86, 332, 332, 431
MflI RGATCY 2 cut(s) 201, 428
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 5 cut(s) 213, 276, 422, 579, 659
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 2 cut(s) 326, 490
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 6 cut(s) 29, 57, 364, 415, 480, 658
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 3 cut(s) 372, 425, 459
Msp20I TGGCCA 1 cut(s) 314
NdeII GATC 5 cut(s) 38, 201, 283, 428, 441
NlaIII CATG 6 cut(s) 115, 347, 481, 515, 554, 675
PfeI GAWTC 3 cut(s) 131, 235, 387
PkrI GCNGC 2 cut(s) 157, 247
PleI GAGTC 2 cut(s) 326, 490
PpsI GAGTC 2 cut(s) 326, 490
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
PsuI RGATCY 2 cut(s) 201, 428
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 372, 425, 459
SatI GCNGC 2 cut(s) 156, 246
Sau3AI GATC 5 cut(s) 38, 201, 283, 428, 441
SchI GAGTC 2 cut(s) 326, 490
SetI ASST 8 cut(s) 13, 82, 92, 160, 291, 491, 533, 669
SfaNI GCATC 2 cut(s) 163, 267
SmlI CTYRAG 2 cut(s) 239, 482
SmoI CTYRAG 2 cut(s) 239, 482
Sse9I AATT 5 cut(s) 213, 276, 422, 579, 659
SsiI CCGC 1 cut(s) 246
SspI AATATT 1 cut(s) 632
SspMI CTAG 1 cut(s) 168
TaiI ACGT 1 cut(s) 92
TasI AATT 5 cut(s) 213, 276, 422, 579, 659
TatI WGTACW 1 cut(s) 108
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 3 cut(s) 131, 235, 387
Tru1I TTAA 3 cut(s) 372, 425, 459
Tru9I TTAA 3 cut(s) 372, 425, 459
TseI GCWGC 1 cut(s) 155
TspDTI ATGAA 5 cut(s) 175, 248, 332, 333, 485
XapI RAATTY 2 cut(s) 213, 579
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.