AT4G02090

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000934
Physical Location & Seq
Forward (+)
923122 .. 923893
772 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02090.1

Sequence Viewer

Length: 609 bp
ATGGGTAACGTTGCTGGTTCCCGAAAGTTGGATGCGGCCGGGATGGCTGCCGGAAAAGTTGTATTATCCGACGGAAGGGTACAAAACTTAGAGGAAGAGACAACGGTGGCTGAGATTATGCTAGAAAATCCGCAACATGTGGTCGTCGAATTTGATCCGTCATCGATATCATTCAACAACGACGCAAAAACGGTGAAGAGAAAGCTGGCTCCTTTGCCCGCGGACAAAACACTCGAGCCAGGGAAAATCTATTTAGTGCTTCCCGCCAAAAGAAGCGGTGGCAGGGCCGCAAAATCATCATCGGCGGTGCTGACGTCAGAGGAGATGCGGAAAATGCTGTTTAGCGCCACGGCAATGGTAAGGTCATCGTTTAGCTACTATGAAGGGATTCTTCCTTGGTTTACTACTAGAAGTTATAAGAACAACAATCCGGCGACTGATACGGTCGTTGCTGCTACGTCGGTTGGAAGATTGGAGGCAGAGATGGAGGAGGAAGATAGGCCGGAGTTTTTGAGCCGGCAATTGTCGGGAAGAGGGTGGAAACCAAGCTTGGATCCTATCAGAGAGAAGAAAGCAAAGAAGAAAATCCATCAACGGTTCTTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

202

Amino Acids

22.32

Weight (kDa)

9.49

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 189 1.2e-28 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 417, 607
AatII GACGTC 1 cut(s) 317
AccII CGCG 1 cut(s) 221
AciI CCGC 9 cut(s) 35, 131, 219, 221, 264, 276, 288, 305, 328
AclI AACGTT 1 cut(s) 9
AclWI GGATC 3 cut(s) 149, 548, 561
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 314
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 28, 75
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 1 cut(s) 238
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 3 cut(s) 205, 375, 549
AluI AGCT 3 cut(s) 205, 375, 549
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 3 cut(s) 149, 548, 561
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 3 cut(s) 36, 285, 500
ApeKI GCWGC 2 cut(s) 47, 452
ApoI RAATTY 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 285
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 233
BaeI ACNNNNGTAYC 2 cut(s) 432, 465
BamHI GGATCC 1 cut(s) 553
BbvI GCAGC 2 cut(s) 34, 439
BccI CCATC 3 cut(s) 37, 478, 597
BceAI ACGGC 1 cut(s) 366
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 2 cut(s) 122, 408
BfoI RGCGCY 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 4 cut(s) 36, 48, 288, 453
BlsI GCNGC 4 cut(s) 37, 49, 289, 454
Bme1390I CCNGG 2 cut(s) 40, 240
BmeT110I CYCGRG 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 3 cut(s) 19, 210, 555
BmrFI CCNGG 2 cut(s) 40, 240
BmsI GCATC 2 cut(s) 22, 315
BpuMI CCSGG 1 cut(s) 40
Bsa29I ATCGAT 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 314
BsaJI CCNNGG 4 cut(s) 219, 239, 348, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 75
Bse118I RCCGGY 1 cut(s) 516
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 4 cut(s) 219, 239, 348, 395
BseGI GGATG 2 cut(s) 37, 48
BseLI CCNNNNNNNGG 2 cut(s) 28, 75
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 102
BseRI GAGGAG 2 cut(s) 335, 503
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 34, 439
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 2 cut(s) 39, 447
BshFI GGCC 3 cut(s) 38, 287, 502
BshVI ATCGAT 1 cut(s) 164
BsiEI CGRYCG 2 cut(s) 39, 447
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 5 cut(s) 39, 51, 431, 503, 517
BslI CCNNNNNNNGG 2 cut(s) 28, 75
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 3 cut(s) 38, 287, 502
BsoBI CYCGRG 1 cut(s) 233
Bsp143I GATC 2 cut(s) 154, 553
BspACI CCGC 9 cut(s) 35, 131, 219, 221, 264, 276, 288, 305, 328
BspANI GGCC 3 cut(s) 38, 287, 502
BspCNI CTCAG 1 cut(s) 103
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 3 cut(s) 19, 210, 555
BspPI GGATC 3 cut(s) 149, 548, 561
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 1 cut(s) 516
BssAI RCCGGY 1 cut(s) 516
BssECI CCNNGG 4 cut(s) 219, 239, 348, 395
BssMI GATC 2 cut(s) 154, 553
BssNI GRCGYC 1 cut(s) 314
BssT1I CCWWGG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 4 cut(s) 106, 193, 445, 597
Bst6I CTCTTC 3 cut(s) 90, 191, 526
BstACI GRCGYC 1 cut(s) 314
BstC8I GCNNGC 3 cut(s) 207, 219, 518
BstDEI CTNAG 2 cut(s) 88, 111
BstDSI CCRYGG 2 cut(s) 219, 348
BstF5I GGATG 2 cut(s) 37, 48
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 2 cut(s) 157, 556
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 2 cut(s) 154, 553
BstMCI CGRYCG 2 cut(s) 39, 447
BstMWI GCNNNNNNNGC 2 cut(s) 44, 334
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 38, 238
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 34, 439
BstX2I RGATCY 1 cut(s) 553
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 1 cut(s) 553
BstZI CGGCCG 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 164
BsuRI GGCC 3 cut(s) 38, 287, 502
BsuTUI ATCGAT 1 cut(s) 164
BtgI CCRYGG 2 cut(s) 219, 348
BtsCI GGATG 2 cut(s) 37, 48
Cac8I GCNNGC 3 cut(s) 207, 219, 518
CfoI GCGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 516
Cfr13I GGNCC 1 cut(s) 285
Cfr42I CCGCGG 1 cut(s) 222
ClaI ATCGAT 1 cut(s) 164
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 80
CviAII CATG 1 cut(s) 137
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 2 cut(s) 88, 111
DpnI GATC 2 cut(s) 156, 555
DpnII GATC 2 cut(s) 154, 553
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 3 cut(s) 90, 191, 526
EarI CTCTTC 3 cut(s) 90, 191, 526
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 395
Eco32I GATATC 1 cut(s) 168
Eco52I CGGCCG 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 238
EcoRV GATATC 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 1 cut(s) 140
FaiI YATR 5 cut(s) 119, 138, 381, 417, 607
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 1 cut(s) 136
FauI CCCGC 2 cut(s) 226, 271
Fnu4HI GCNGC 4 cut(s) 36, 48, 288, 453
FokI GGATG 2 cut(s) 44, 55
Fsp4HI GCNGC 4 cut(s) 36, 48, 288, 453
FspBI CTAG 2 cut(s) 122, 408
GlaI GCGC 1 cut(s) 346
GluI GCNGC 4 cut(s) 36, 48, 288, 453
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 3 cut(s) 38, 287, 502
HapII CCGG 5 cut(s) 39, 51, 431, 503, 517
HgaI GACGC 1 cut(s) 191
HhaI GCGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 314
Hin1II CATG 1 cut(s) 140
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 547
HinfI GANTC 1 cut(s) 388
HpaII CCGG 5 cut(s) 39, 51, 431, 503, 517
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 1 cut(s) 402
Hpy188I TCNGA 3 cut(s) 70, 319, 563
Hpy188III TCNNGA 2 cut(s) 21, 528
Hpy8I GTNNAC 1 cut(s) 402
Hpy99I CGWCG 4 cut(s) 74, 149, 185, 463
HpyAV CCTTC 2 cut(s) 69, 377
HpyCH4III ACNGT 4 cut(s) 106, 193, 445, 597
HpyCH4IV ACGT 3 cut(s) 9, 314, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 334
HpyF3I CTNAG 2 cut(s) 88, 111
HpySE526I ACGT 3 cut(s) 9, 314, 458
Hsp92I GRCGYC 1 cut(s) 314
Hsp92II CATG 1 cut(s) 140
HspAI GCGC 1 cut(s) 345
KroI GCCGGC 1 cut(s) 516
KroNI GCCGGC 1 cut(s) 518
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 2 cut(s) 154, 553
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 9 cut(s) 52, 64, 191, 225, 252, 268, 444, 516, 530
Lsp1109I GCAGC 2 cut(s) 34, 439
LweI GCATC 2 cut(s) 22, 315
MaeI CTAG 2 cut(s) 122, 408
MaeII ACGT 3 cut(s) 9, 314, 458
MaeIII GTNAC 1 cut(s) 5
MalI GATC 2 cut(s) 156, 555
MboI GATC 2 cut(s) 154, 553
MboII GAAGA 8 cut(s) 107, 208, 383, 480, 506, 543, 580, 592
MfeI CAATTG 1 cut(s) 521
MflI RGATCY 1 cut(s) 553
MluCI AATT 2 cut(s) 149, 521
MmeI TCCRAC 3 cut(s) 9, 93, 445
MnlI CCTC 6 cut(s) 85, 313, 469, 481, 484, 527
MroNI GCCGGC 1 cut(s) 516
MroXI GAANNNNTTC 1 cut(s) 387
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 5 cut(s) 39, 51, 431, 503, 517
MspR9I CCNGG 2 cut(s) 40, 240
MunI CAATTG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 44, 334
NaeI GCCGGC 1 cut(s) 518
NciI CCSGG 1 cut(s) 40
NdeII GATC 2 cut(s) 154, 553
NgoMIV GCCGGC 1 cut(s) 516
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 3 cut(s) 19, 210, 555
NspI RCATGY 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 233
PciI ACATGT 1 cut(s) 136
PdiI GCCGGC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 388
PkrI GCNGC 4 cut(s) 37, 49, 289, 454
PscI ACATGT 1 cut(s) 136
PsiI TTATAA 2 cut(s) 417, 607
Psp1406I AACGTT 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 3 cut(s) 19, 210, 555
PspPI GGNCC 1 cut(s) 285
PspXI VCTCGAGB 1 cut(s) 233
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 353
SacII CCGCGG 1 cut(s) 222
SatI GCNGC 4 cut(s) 36, 48, 288, 453
Sau3AI GATC 2 cut(s) 154, 553
Sau96I GGNCC 1 cut(s) 285
ScrFI CCNGG 2 cut(s) 40, 240
SetI ASST 7 cut(s) 12, 207, 317, 365, 377, 461, 551
SfaNI GCATC 2 cut(s) 22, 315
Sfr274I CTCGAG 1 cut(s) 233
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 2 cut(s) 149, 521
SsiI CCGC 9 cut(s) 35, 131, 219, 221, 264, 276, 288, 305, 328
SspMI CTAG 2 cut(s) 122, 408
StyD4I CCNGG 2 cut(s) 38, 238
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 4 cut(s) 106, 193, 445, 597
TaiI ACGT 3 cut(s) 12, 317, 461
TaqI TCGA 3 cut(s) 147, 164, 234
TasI AATT 2 cut(s) 149, 521
TauI GCSGC 2 cut(s) 38, 290
TfiI GAWTC 1 cut(s) 388
TseI GCWGC 2 cut(s) 47, 452
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 2 cut(s) 87, 147
XapI RAATTY 1 cut(s) 149
XceI RCATGY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 2 cut(s) 122, 408
ZraI GACGTC 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.