Histidine kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 129, 2535 |
| AccB1I | GGYRCC | 1 cut(s) | 1594 |
| AccII | CGCG | 2 cut(s) | 2418, 2429 |
| AccIII | TCCGGA | 1 cut(s) | 1942 |
| AciI | CCGC | 4 cut(s) | 692, 1915, 2332, 2418 |
| AclWI | GGATC | 6 cut(s) | 650, 663, 1345, 1351, 1788, 1868 |
| AcsI | RAATTY | 9 cut(s) | 466, 572, 752, 932, 966, 1386, 1397, 1769, 2300 |
| AfaI | GTAC | 6 cut(s) | 72, 863, 1077, 1261, 1321, 1798 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 665, 787, 1949, 2291, 2599 |
| AflII | CTTAAG | 1 cut(s) | 713 |
| AflIII | ACRYGT | 2 cut(s) | 769, 2263 |
| AhlI | ACTAGT | 1 cut(s) | 1240 |
| AjnI | CCWGG | 4 cut(s) | 445, 1222, 1292, 1998 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1011, 1043, 1200, 1232 |
| Alw21I | GWGCWC | 2 cut(s) | 2023, 2425 |
| Alw26I | GTCTC | 6 cut(s) | 455, 1180, 1927, 2138, 2199, 2659 |
| AlwI | GGATC | 6 cut(s) | 650, 663, 1345, 1351, 1788, 1868 |
| Aor13HI | TCCGGA | 1 cut(s) | 1942 |
| AoxI | GGCC | 4 cut(s) | 318, 1219, 1974, 2282 |
| ApeKI | GCWGC | 5 cut(s) | 392, 821, 1641, 1644, 1987 |
| ApoI | RAATTY | 9 cut(s) | 466, 572, 752, 932, 966, 1386, 1397, 1769, 2300 |
| AspLEI | GCGC | 3 cut(s) | 586, 1610, 1903 |
| AspS9I | GGNCC | 4 cut(s) | 1220, 1736, 1974, 2283 |
| AsuC2I | CCSGG | 1 cut(s) | 1266 |
| AsuHPI | GGTGA | 5 cut(s) | 710, 1238, 1778, 1942, 2174 |
| AsuII | TTCGAA | 2 cut(s) | 1306, 2496 |
| AsuNHI | GCTAGC | 1 cut(s) | 2338 |
| AvaII | GGWCC | 1 cut(s) | 1736 |
| AxyI | CCTNAGG | 1 cut(s) | 1326 |
| BamHI | GGATCC | 1 cut(s) | 655 |
| BanI | GGYRCC | 1 cut(s) | 1594 |
| BanII | GRGCYC | 1 cut(s) | 2023 |
| BbsI | GAAGAC | 2 cut(s) | 280, 715 |
| Bbv12I | GWGCWC | 2 cut(s) | 2023, 2425 |
| BbvI | GCAGC | 5 cut(s) | 379, 808, 1653, 1656, 1999 |
| BccI | CCATC | 7 cut(s) | 472, 659, 1111, 1328, 2483, 2558, 2593 |
| BceAI | ACGGC | 1 cut(s) | 1038 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 98, 132 |
| BciT130I | CCWGG | 4 cut(s) | 447, 1224, 1294, 2000 |
| BciVI | GTATCC | 1 cut(s) | 1230 |
| BclI | TGATCA | 3 cut(s) | 303, 1602, 2074 |
| BcnI | CCSGG | 1 cut(s) | 1266 |
| BcoDI | GTCTC | 6 cut(s) | 455, 1180, 1927, 2138, 2199, 2659 |
| BcuI | ACTAGT | 1 cut(s) | 1240 |
| BfoI | RGCGCY | 1 cut(s) | 1904 |
| BfrI | CTTAAG | 1 cut(s) | 713 |
| BfuI | GTATCC | 1 cut(s) | 1230 |
| BglII | AGATCT | 2 cut(s) | 496, 2407 |
| BisI | GCNGC | 6 cut(s) | 393, 822, 1642, 1645, 1915, 1988 |
| BlsI | GCNGC | 6 cut(s) | 394, 823, 1643, 1646, 1916, 1989 |
| BmcAI | AGTACT | 3 cut(s) | 1261, 1321, 1798 |
| Bme1390I | CCNGG | 5 cut(s) | 447, 1224, 1266, 1294, 2000 |
| Bme18I | GGWCC | 1 cut(s) | 1736 |
| BmgT120I | GGNCC | 4 cut(s) | 1220, 1736, 1974, 2283 |
| BmiI | GGNNCC | 6 cut(s) | 451, 657, 1596, 1738, 2206, 2529 |
| BmrFI | CCNGG | 5 cut(s) | 447, 1224, 1266, 1294, 2000 |
| BmsI | GCATC | 7 cut(s) | 102, 273, 1074, 1714, 1854, 2466, 2582 |
| BmtI | GCTAGC | 1 cut(s) | 2342 |
| BpiI | GAAGAC | 2 cut(s) | 280, 715 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1160, 1192 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1048 |
| Bpu14I | TTCGAA | 2 cut(s) | 1306, 2496 |
| BpuEI | CTTGAG | 2 cut(s) | 161, 1570 |
| BpuMI | CCSGG | 1 cut(s) | 1266 |
| Bsa29I | ATCGAT | 1 cut(s) | 2053 |
| BsaAI | YACGTR | 2 cut(s) | 256, 1166 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1342 |
| BsaI | GGTCTC | 1 cut(s) | 2138 |
| BsaJI | CCNNGG | 7 cut(s) | 445, 759, 927, 1179, 1222, 1292, 1411 |
| BsaWI | WCCGGW | 1 cut(s) | 1942 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1237, 1267 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 665, 787, 1949, 2291, 2599 |
| Bse1I | ACTGG | 1 cut(s) | 1048 |
| Bse21I | CCTNAGG | 1 cut(s) | 1326 |
| Bse3DI | GCAATG | 4 cut(s) | 748, 844, 1617, 2553 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1342 |
| BseAI | TCCGGA | 1 cut(s) | 1942 |
| BseBI | CCWGG | 4 cut(s) | 447, 1224, 1294, 2000 |
| BseCI | ATCGAT | 1 cut(s) | 2053 |
| BseDI | CCNNGG | 7 cut(s) | 445, 759, 927, 1179, 1222, 1292, 1411 |
| BseGI | GGATG | 7 cut(s) | 198, 616, 955, 1346, 1888, 2494, 2604 |
| BseJI | GATNNNNATC | 1 cut(s) | 1342 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 665, 787, 1949, 2291, 2599 |
| BseMI | GCAATG | 4 cut(s) | 748, 844, 1617, 2553 |
| BseMII | CTCAG | 1 cut(s) | 1518 |
| BseNI | ACTGG | 1 cut(s) | 1048 |
| BseRI | GAGGAG | 4 cut(s) | 194, 2197, 2537, 2540 |
| BseXI | GCAGC | 5 cut(s) | 379, 808, 1653, 1656, 1999 |
| BseYI | CCCAGC | 3 cut(s) | 545, 800, 2362 |
| BsgI | GTGCAG | 1 cut(s) | 1837 |
| Bsh1236I | CGCG | 2 cut(s) | 2418, 2429 |
| BshFI | GGCC | 4 cut(s) | 320, 1221, 1976, 2284 |
| BshNI | GGYRCC | 1 cut(s) | 1594 |
| BshVI | ATCGAT | 1 cut(s) | 2053 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2023, 2425 |
| BsiSI | CCGG | 4 cut(s) | 402, 1266, 1943, 2480 |
| BslFI | GGGAC | 1 cut(s) | 780 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 665, 787, 1949, 2291, 2599 |
| BsmAI | GTCTC | 6 cut(s) | 455, 1180, 1927, 2138, 2199, 2659 |
| BsmBI | CGTCTC | 1 cut(s) | 2659 |
| BsmFI | GGGAC | 1 cut(s) | 780 |
| BsmI | GAATGC | 2 cut(s) | 194, 1275 |
| BsnI | GGCC | 4 cut(s) | 320, 1221, 1976, 2284 |
| Bso31I | GGTCTC | 1 cut(s) | 2138 |
| Bsp119I | TTCGAA | 2 cut(s) | 1306, 2496 |
| Bsp1286I | GDGCHC | 2 cut(s) | 2023, 2425 |
| Bsp13I | TCCGGA | 1 cut(s) | 1942 |
| Bsp68I | TCGCGA | 1 cut(s) | 2429 |
| BspACI | CCGC | 4 cut(s) | 692, 1915, 2332, 2418 |
| BspANI | GGCC | 4 cut(s) | 320, 1221, 1976, 2284 |
| BspCNI | CTCAG | 1 cut(s) | 1519 |
| BspDI | ATCGAT | 1 cut(s) | 2053 |
| BspEI | TCCGGA | 1 cut(s) | 1942 |
| BspFNI | CGCG | 2 cut(s) | 2418, 2429 |
| BspLI | GGNNCC | 6 cut(s) | 451, 657, 1596, 1738, 2206, 2529 |
| BspOI | GCTAGC | 1 cut(s) | 2342 |
| BspPI | GGATC | 6 cut(s) | 650, 663, 1345, 1351, 1788, 1868 |
| BspQI | GCTCTTC | 2 cut(s) | 1368, 1519 |
| BspT104I | TTCGAA | 2 cut(s) | 1306, 2496 |
| BspT107I | GGYRCC | 1 cut(s) | 1594 |
| BspTI | CTTAAG | 1 cut(s) | 713 |
| BspTNI | GGTCTC | 1 cut(s) | 2138 |
| BsrDI | GCAATG | 4 cut(s) | 748, 844, 1617, 2553 |
| BsrI | ACTGG | 1 cut(s) | 1048 |
| BssECI | CCNNGG | 7 cut(s) | 445, 759, 927, 1179, 1222, 1292, 1411 |
| BssT1I | CCWWGG | 3 cut(s) | 927, 1179, 1411 |
| Bst2UI | CCWGG | 4 cut(s) | 447, 1224, 1294, 2000 |
| Bst4CI | ACNGT | 4 cut(s) | 778, 858, 2374, 2634 |
| Bst6I | CTCTTC | 5 cut(s) | 561, 1368, 1506, 1519, 2389 |
| BstAFI | CTTAAG | 1 cut(s) | 713 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1815 |
| BstBAI | YACGTR | 2 cut(s) | 256, 1166 |
| BstBI | TTCGAA | 2 cut(s) | 1306, 2496 |
| BstC8I | GCNNGC | 7 cut(s) | 54, 65, 79, 376, 2336, 2340, 2608 |
| BstDEI | CTNAG | 4 cut(s) | 1048, 1326, 1527, 1965 |
| BstEII | GGTNACC | 1 cut(s) | 1490 |
| BstF5I | GGATG | 7 cut(s) | 198, 616, 955, 1346, 1888, 2494, 2604 |
| BstFNI | CGCG | 2 cut(s) | 2418, 2429 |
| BstH2I | RGCGCY | 1 cut(s) | 1904 |
| BstHHI | GCGC | 3 cut(s) | 586, 1610, 1903 |
| BstMAI | GTCTC | 6 cut(s) | 455, 1180, 1927, 2138, 2199, 2659 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 31, 109, 228, 398, 1128, 1575, 1815, 2018 |
| BstNI | CCWGG | 4 cut(s) | 447, 1224, 1294, 2000 |
| BstNSI | RCATGY | 6 cut(s) | 38, 81, 773, 1137, 1657, 2267 |
| BstPI | GGTNACC | 1 cut(s) | 1490 |
| BstSCI | CCNGG | 5 cut(s) | 445, 1222, 1264, 1292, 1998 |
| BstSNI | TACGTA | 1 cut(s) | 256 |
| BstUI | CGCG | 2 cut(s) | 2418, 2429 |
| BstV1I | GCAGC | 5 cut(s) | 379, 808, 1653, 1656, 1999 |
| BstV2I | GAAGAC | 2 cut(s) | 280, 715 |
| BstX2I | RGATCY | 3 cut(s) | 496, 655, 2407 |
| BstYI | RGATCY | 3 cut(s) | 496, 655, 2407 |
| Bsu15I | ATCGAT | 1 cut(s) | 2053 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1326 |
| BsuI | GTATCC | 1 cut(s) | 1230 |
| BsuRI | GGCC | 4 cut(s) | 320, 1221, 1976, 2284 |
| BsuTUI | ATCGAT | 1 cut(s) | 2053 |
| BtsCI | GGATG | 7 cut(s) | 198, 616, 955, 1346, 1888, 2494, 2604 |
| BtsIMutI | CAGTG | 2 cut(s) | 996, 1041 |
| BtuMI | TCGCGA | 1 cut(s) | 2429 |
| Cac8I | GCNNGC | 7 cut(s) | 54, 65, 79, 376, 2336, 2340, 2608 |
| CfoI | GCGC | 3 cut(s) | 586, 1610, 1903 |
| Cfr13I | GGNCC | 4 cut(s) | 1220, 1736, 1974, 2283 |
| ClaI | ATCGAT | 1 cut(s) | 2053 |
| CseI | GACGC | 3 cut(s) | 1367, 2001, 2676 |
| CsiI | ACCWGGT | 1 cut(s) | 1998 |
| Csp6I | GTAC | 6 cut(s) | 71, 862, 1076, 1260, 1320, 1797 |
| CviQI | GTAC | 6 cut(s) | 71, 862, 1076, 1260, 1320, 1797 |
| DdeI | CTNAG | 4 cut(s) | 1048, 1326, 1527, 1965 |
| DraI | TTTAAA | 1 cut(s) | 1675 |
| Eam1104I | CTCTTC | 5 cut(s) | 561, 1368, 1506, 1519, 2389 |
| EarI | CTCTTC | 5 cut(s) | 561, 1368, 1506, 1519, 2389 |
| Ecl136II | GAGCTC | 1 cut(s) | 2021 |
| Eco105I | TACGTA | 1 cut(s) | 256 |
| Eco130I | CCWWGG | 3 cut(s) | 927, 1179, 1411 |
| Eco24I | GRGCYC | 1 cut(s) | 2023 |
| Eco31I | GGTCTC | 1 cut(s) | 2138 |
| Eco32I | GATATC | 1 cut(s) | 426 |
| Eco47I | GGWCC | 1 cut(s) | 1736 |
| Eco53kI | GAGCTC | 1 cut(s) | 2021 |
| Eco81I | CCTNAGG | 1 cut(s) | 1326 |
| Eco91I | GGTNACC | 1 cut(s) | 1490 |
| EcoICRI | GAGCTC | 1 cut(s) | 2021 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2283 |
| EcoO65I | GGTNACC | 1 cut(s) | 1490 |
| EcoRI | GAATTC | 3 cut(s) | 572, 966, 1769 |
| EcoRII | CCWGG | 4 cut(s) | 445, 1222, 1292, 1998 |
| EcoRV | GATATC | 1 cut(s) | 426 |
| EcoT14I | CCWWGG | 3 cut(s) | 927, 1179, 1411 |
| EcoT22I | ATGCAT | 3 cut(s) | 264, 288, 1089 |
| EcoT38I | GRGCYC | 1 cut(s) | 2023 |
| ErhI | CCWWGG | 3 cut(s) | 927, 1179, 1411 |
| Esp3I | CGTCTC | 1 cut(s) | 2659 |
| FaqI | GGGAC | 1 cut(s) | 780 |
| FbaI | TGATCA | 3 cut(s) | 303, 1602, 2074 |
| Fnu4HI | GCNGC | 6 cut(s) | 393, 822, 1642, 1645, 1915, 1988 |
| FokI | GGATG | 7 cut(s) | 185, 603, 962, 1333, 1895, 2501, 2611 |
| FriOI | GRGCYC | 1 cut(s) | 2023 |
| Fsp4HI | GCNGC | 6 cut(s) | 393, 822, 1642, 1645, 1915, 1988 |
| GlaI | GCGC | 3 cut(s) | 585, 1609, 1902 |
| GluI | GCNGC | 6 cut(s) | 393, 822, 1642, 1645, 1915, 1988 |
| GsaI | CCCAGC | 3 cut(s) | 549, 804, 2366 |
| HaeII | RGCGCY | 1 cut(s) | 1904 |
| HaeIII | GGCC | 4 cut(s) | 320, 1221, 1976, 2284 |
| HapII | CCGG | 4 cut(s) | 402, 1266, 1943, 2480 |
| HgaI | GACGC | 3 cut(s) | 1367, 2001, 2676 |
| HhaI | GCGC | 3 cut(s) | 586, 1610, 1903 |
| Hin6I | GCGC | 3 cut(s) | 584, 1608, 1901 |
| HinP1I | GCGC | 3 cut(s) | 584, 1608, 1901 |
| HincII | GTYRAC | 1 cut(s) | 607 |
| HindII | GTYRAC | 1 cut(s) | 607 |
| HinfI | GANTC | 5 cut(s) | 405, 2179, 2230, 2431, 2448 |
| HpaII | CCGG | 4 cut(s) | 402, 1266, 1943, 2480 |
| HphI | GGTGA | 5 cut(s) | 710, 1238, 1778, 1942, 2174 |
| Hpy166II | GTNNAC | 4 cut(s) | 607, 1108, 1201, 1588 |
| Hpy188I | TCNGA | 6 cut(s) | 454, 763, 1257, 1528, 1747, 2277 |
| Hpy8I | GTNNAC | 4 cut(s) | 607, 1108, 1201, 1588 |
| Hpy99I | CGWCG | 2 cut(s) | 1355, 1383 |
| HpyAV | CCTTC | 5 cut(s) | 955, 1028, 1697, 1965, 2589 |
| HpyCH4III | ACNGT | 4 cut(s) | 778, 858, 2374, 2634 |
| HpyCH4IV | ACGT | 3 cut(s) | 255, 1078, 1165 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 31, 109, 228, 398, 1128, 1575, 1815, 2018 |
| HpyF3I | CTNAG | 4 cut(s) | 1048, 1326, 1527, 1965 |
| HpySE526I | ACGT | 3 cut(s) | 255, 1078, 1165 |
| HspAI | GCGC | 3 cut(s) | 584, 1608, 1901 |
| Kpn2I | TCCGGA | 1 cut(s) | 1942 |
| Ksp22I | TGATCA | 3 cut(s) | 303, 1602, 2074 |
| LguI | GCTCTTC | 2 cut(s) | 1368, 1519 |
| LmnI | GCTCC | 5 cut(s) | 1662, 2026, 2210, 2377, 2527 |
| Lsp1109I | GCAGC | 5 cut(s) | 379, 808, 1653, 1656, 1999 |
| LweI | GCATC | 7 cut(s) | 102, 273, 1074, 1714, 1854, 2466, 2582 |
| MabI | ACCWGGT | 1 cut(s) | 1998 |
| MaeII | ACGT | 3 cut(s) | 255, 1078, 1165 |
| MaeIII | GTNAC | 8 cut(s) | 698, 728, 772, 1243, 1471, 1490, 1762, 2567 |
| MfeI | CAATTG | 1 cut(s) | 1810 |
| MflI | RGATCY | 3 cut(s) | 496, 655, 2407 |
| MhlI | GDGCHC | 2 cut(s) | 2023, 2425 |
| MlyI | GAGTC | 1 cut(s) | 2173 |
| MmeI | TCCRAC | 4 cut(s) | 438, 580, 633, 1984 |
| Mph1103I | ATGCAT | 3 cut(s) | 264, 288, 1089 |
| MroI | TCCGGA | 1 cut(s) | 1942 |
| MslI | CAYNNNNRTG | 3 cut(s) | 212, 644, 1208 |
| MspA1I | CMGCKG | 1 cut(s) | 1733 |
| MspCI | CTTAAG | 1 cut(s) | 713 |
| MspI | CCGG | 4 cut(s) | 402, 1266, 1943, 2480 |
| MspR9I | CCNGG | 5 cut(s) | 447, 1224, 1266, 1294, 2000 |
| MunI | CAATTG | 1 cut(s) | 1810 |
| Mva1269I | GAATGC | 2 cut(s) | 194, 1275 |
| MvaI | CCWGG | 4 cut(s) | 447, 1224, 1294, 2000 |
| MvnI | CGCG | 2 cut(s) | 2418, 2429 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 31, 109, 228, 398, 1128, 1575, 1815, 2018 |
| NciI | CCSGG | 1 cut(s) | 1266 |
| NheI | GCTAGC | 1 cut(s) | 2338 |
| NlaIV | GGNNCC | 6 cut(s) | 451, 657, 1596, 1738, 2206, 2529 |
| NmuCI | GTSAC | 5 cut(s) | 698, 772, 1243, 1762, 2567 |
| NruI | TCGCGA | 1 cut(s) | 2429 |
| NsiI | ATGCAT | 3 cut(s) | 264, 288, 1089 |
| NspI | RCATGY | 6 cut(s) | 38, 81, 773, 1137, 1657, 2267 |
| NspV | TTCGAA | 2 cut(s) | 1306, 2496 |
| PaeI | GCATGC | 1 cut(s) | 81 |
| PciI | ACATGT | 2 cut(s) | 769, 2263 |
| PciSI | GCTCTTC | 2 cut(s) | 1368, 1519 |
| PctI | GAATGC | 2 cut(s) | 194, 1275 |
| PfeI | GAWTC | 4 cut(s) | 405, 2230, 2431, 2448 |
| PfoI | TCCNGGA | 1 cut(s) | 1264 |
| PkrI | GCNGC | 6 cut(s) | 394, 823, 1643, 1646, 1916, 1989 |
| PleI | GAGTC | 1 cut(s) | 2173 |
| PpsI | GAGTC | 1 cut(s) | 2173 |
| Ppu21I | YACGTR | 2 cut(s) | 256, 1166 |
| PscI | ACATGT | 2 cut(s) | 769, 2263 |
| PsiI | TTATAA | 2 cut(s) | 129, 2535 |
| Psp124BI | GAGCTC | 1 cut(s) | 2023 |
| Psp6I | CCWGG | 4 cut(s) | 445, 1222, 1292, 1998 |
| PspEI | GGTNACC | 1 cut(s) | 1490 |
| PspFI | CCCAGC | 3 cut(s) | 545, 800, 2362 |
| PspGI | CCWGG | 4 cut(s) | 445, 1222, 1292, 1998 |
| PspN4I | GGNNCC | 6 cut(s) | 451, 657, 1596, 1738, 2206, 2529 |
| PspPI | GGNCC | 4 cut(s) | 1220, 1736, 1974, 2283 |
| PsuI | RGATCY | 3 cut(s) | 496, 655, 2407 |
| PvuII | CAGCTG | 1 cut(s) | 1733 |
| RruI | TCGCGA | 1 cut(s) | 2429 |
| RsaI | GTAC | 6 cut(s) | 72, 863, 1077, 1261, 1321, 1798 |
| RsaNI | GTAC | 6 cut(s) | 71, 862, 1076, 1260, 1320, 1797 |
| RseI | CAYNNNNRTG | 3 cut(s) | 212, 644, 1208 |
| SacI | GAGCTC | 1 cut(s) | 2023 |
| SapI | GCTCTTC | 2 cut(s) | 1368, 1519 |
| SatI | GCNGC | 6 cut(s) | 393, 822, 1642, 1645, 1915, 1988 |
| Sau96I | GGNCC | 4 cut(s) | 1220, 1736, 1974, 2283 |
| ScaI | AGTACT | 3 cut(s) | 1261, 1321, 1798 |
| SchI | GAGTC | 1 cut(s) | 2173 |
| ScrFI | CCNGG | 5 cut(s) | 447, 1224, 1266, 1294, 2000 |
| SduI | GDGCHC | 2 cut(s) | 2023, 2425 |
| SexAI | ACCWGGT | 1 cut(s) | 1998 |
| SfaNI | GCATC | 7 cut(s) | 102, 273, 1074, 1714, 1854, 2466, 2582 |
| SfuI | TTCGAA | 2 cut(s) | 1306, 2496 |
| SinI | GGWCC | 1 cut(s) | 1736 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 212, 644, 1208 |
| SmlI | CTYRAG | 3 cut(s) | 176, 713, 1549 |
| SmoI | CTYRAG | 3 cut(s) | 176, 713, 1549 |
| SnaBI | TACGTA | 1 cut(s) | 256 |
| SpeI | ACTAGT | 1 cut(s) | 1240 |
| SphI | GCATGC | 1 cut(s) | 81 |
| SsiI | CCGC | 4 cut(s) | 692, 1915, 2332, 2418 |
| SspI | AATATT | 2 cut(s) | 2122, 2163 |
| SstI | GAGCTC | 1 cut(s) | 2023 |
| StyD4I | CCNGG | 5 cut(s) | 445, 1222, 1264, 1292, 1998 |
| StyI | CCWWGG | 3 cut(s) | 927, 1179, 1411 |
| TaaI | ACNGT | 4 cut(s) | 778, 858, 2374, 2634 |
| TaiI | ACGT | 3 cut(s) | 258, 1081, 1168 |
| TaqI | TCGA | 9 cut(s) | 108, 576, 792, 942, 1306, 1690, 2053, 2451, 2496 |
| TatI | WGTACW | 4 cut(s) | 861, 1259, 1319, 1796 |
| TauI | GCSGC | 1 cut(s) | 1917 |
| TfiI | GAWTC | 4 cut(s) | 405, 2230, 2431, 2448 |
| TscAI | CASTG | 2 cut(s) | 996, 1048 |
| TseFI | GTSAC | 5 cut(s) | 698, 772, 1243, 1762, 2567 |
| TseI | GCWGC | 5 cut(s) | 392, 821, 1641, 1644, 1987 |
| Tsp45I | GTSAC | 5 cut(s) | 698, 772, 1243, 1762, 2567 |
| TspGWI | ACGGA | 4 cut(s) | 675, 770, 1339, 1793 |
| TspRI | CASTG | 2 cut(s) | 996, 1048 |
| Vha464I | CTTAAG | 1 cut(s) | 713 |
| VpaK11BI | GGWCC | 1 cut(s) | 1736 |
| XapI | RAATTY | 9 cut(s) | 466, 572, 752, 932, 966, 1386, 1397, 1769, 2300 |
| XbaI | TCTAGA | 3 cut(s) | 1462, 2015, 2404 |
| XceI | RCATGY | 6 cut(s) | 38, 81, 773, 1137, 1657, 2267 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2120 |
| ZrmI | AGTACT | 3 cut(s) | 1261, 1321, 1798 |
| Zsp2I | ATGCAT | 3 cut(s) | 264, 288, 1089 |