pycom03g23460

Multidrug resistance protein ABC transporter family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000934
Physical Location & Seq
Forward (+)
23263552 .. 23264145
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23460.1

Sequence Viewer

Length: 594 bp
ATGGGCAATTCCATCTCCCACAGGAAATGCCACGCCACCGGAAAAGTCATACTATCTGACGGCTCTGTGCATGAGTTCAATAAGCCCCTCACCGTTGCCGAGCTCATGCTCGAGAACCCGCAGCAAGTTGTTGTGGAATTCGGCTCTGCTGTCTCCCAAAAAAGGGCAACCCCGTTGCCTGCAGACATGAAGCTCGACATGAAAAAGGTTTACTTGATGCTGCCAATGAAGCGAGGGAAACCACTGTCGTTGTCATCCGAAGAAGTCCGCGGTGTTCTTTTAAGTGCAAATTTGGTTTTACGATCGAGGTCGCTAACGTTTTTACCTTTGTTTGCTAGAATATGCCCGGCAGGCGATCCCGGGCATGCCTTTGTGCTGCCAAACAAGGACAAAGAATCTAGTATGGAGAGGACGTCGTCCAAGGTCGTGAGCAGGCATGTGCCGGAGTTTGATCTGCCGGAAGATTTGGACTCGAGACCGGACTATTTGAGTAGGCAGCTTTCGGGCAAGAGTACTTGGAAACCGAACTTGGATACCATTAAGGAGAAGAAGGCTGACAAGAAGTCTCATTGGTTATTTTCAACTTTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

198

Amino Acids

22.02

Weight (kDa)

9.61

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 182 1.6e-28 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 416
AccII CGCG 1 cut(s) 270
AciI CCGC 3 cut(s) 119, 268, 270
AclI AACGTT 1 cut(s) 317
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 2 cut(s) 137, 289
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 1 cut(s) 514
AfiI CCNNNNNNNGG 2 cut(s) 162, 163
AgsI TTSAA 3 cut(s) 79, 582, 589
AhdI GACNNNNNGTC 1 cut(s) 562
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 3 cut(s) 103, 193, 499
AluI AGCT 3 cut(s) 103, 193, 499
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 3 cut(s) 157, 469, 570
AlwI GGATC 1 cut(s) 350
Ama87I CYCGRG 3 cut(s) 110, 359, 472
ApeKI GCWGC 4 cut(s) 121, 220, 376, 496
ApoI RAATTY 2 cut(s) 137, 289
AsuC2I CCSGG 3 cut(s) 347, 360, 361
AsuHPI GGTGA 1 cut(s) 82
AvaI CYCGRG 3 cut(s) 110, 359, 472
BanII GRGCYC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 4 cut(s) 133, 207, 363, 508
BccI CCATC 1 cut(s) 20
BceAI ACGGC 1 cut(s) 76
BciVI GTATCC 1 cut(s) 526
BcnI CCSGG 3 cut(s) 347, 360, 361
BcoDI GTCTC 3 cut(s) 157, 469, 570
BfaI CTAG 2 cut(s) 336, 399
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 351
BisI GCNGC 4 cut(s) 122, 221, 377, 497
BlsI GCNGC 4 cut(s) 123, 222, 378, 498
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 3 cut(s) 347, 360, 361
BmeRI GACNNNNNGTC 1 cut(s) 562
BmeT110I CYCGRG 3 cut(s) 110, 359, 472
BmrFI CCNGG 3 cut(s) 347, 360, 361
BmsI GCATC 1 cut(s) 207
BpuMI CCSGG 3 cut(s) 347, 360, 361
BsaHI GRCGYC 1 cut(s) 413
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 3 cut(s) 268, 359, 420
BsaWI WCCGGW 2 cut(s) 38, 478
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 163
BseDI CCNNGG 3 cut(s) 268, 359, 420
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 162, 163
BseXI GCAGC 4 cut(s) 133, 207, 363, 508
Bsh1236I CGCG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 3 cut(s) 110, 359, 472
BsiSI CCGG 6 cut(s) 39, 347, 360, 443, 458, 479
BslI CCNNNNNNNGG 2 cut(s) 162, 163
BsmAI GTCTC 3 cut(s) 157, 469, 570
Bso31I GGTCTC 1 cut(s) 469
BsoBI CYCGRG 3 cut(s) 110, 359, 472
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 3 cut(s) 302, 355, 451
BspACI CCGC 3 cut(s) 119, 268, 270
BspFNI CGCG 1 cut(s) 270
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 268, 359, 420
BssMI GATC 3 cut(s) 302, 355, 451
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 94, 246
BstACI GRCGYC 1 cut(s) 413
BstC8I GCNNGC 4 cut(s) 180, 352, 366, 434
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 254
BstFNI CGCG 1 cut(s) 270
BstKTI GATC 3 cut(s) 305, 358, 454
BstMAI GTCTC 3 cut(s) 157, 469, 570
BstMBI GATC 3 cut(s) 302, 355, 451
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 2 cut(s) 229, 351
BstNSI RCATGY 2 cut(s) 368, 440
BstSCI CCNGG 3 cut(s) 345, 358, 359
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 4 cut(s) 133, 207, 363, 508
BsuI GTATCC 1 cut(s) 526
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 1 cut(s) 254
BtsIMutI CAGTG 1 cut(s) 242
Cac8I GCNNGC 4 cut(s) 180, 352, 366, 434
Cfr42I CCGCGG 1 cut(s) 271
Cfr9I CCCGGG 1 cut(s) 359
Csp6I GTAC 1 cut(s) 513
CspCI CAANNNNNGTGG 2 cut(s) 231, 266
CviAII CATG 6 cut(s) 71, 106, 187, 199, 365, 437
CviJI RGCY 7 cut(s) 63, 85, 103, 144, 193, 499, 554
CviKI_1 RGCY 7 cut(s) 63, 85, 103, 144, 193, 499, 554
CviQI GTAC 1 cut(s) 513
DpnI GATC 3 cut(s) 304, 357, 453
DpnII GATC 3 cut(s) 302, 355, 451
DriI GACNNNNNGTC 1 cut(s) 562
Eam1105I GACNNNNNGTC 1 cut(s) 562
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 103
Eco88I CYCGRG 3 cut(s) 110, 359, 472
EcoICRI GAGCTC 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 6 cut(s) 74, 109, 190, 202, 368, 440
FaiI YATR 9 cut(s) 50, 72, 107, 188, 200, 343, 366, 404, 438
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 6 cut(s) 70, 105, 186, 198, 364, 436
FauI CCCGC 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 122, 221, 377, 497
FokI GGATG 1 cut(s) 241
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 4 cut(s) 122, 221, 377, 497
FspBI CTAG 2 cut(s) 336, 399
GluI GCNGC 4 cut(s) 122, 221, 377, 497
HapII CCGG 6 cut(s) 39, 347, 360, 443, 458, 479
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 6 cut(s) 74, 109, 190, 202, 368, 440
HinfI GANTC 2 cut(s) 395, 470
HpaII CCGG 6 cut(s) 39, 347, 360, 443, 458, 479
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 58, 259
Hpy188III TCNNGA 3 cut(s) 112, 427, 474
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 544
HpyCH4III ACNGT 2 cut(s) 94, 246
HpyCH4IV ACGT 2 cut(s) 317, 413
HpyCH4V TGCA 3 cut(s) 70, 182, 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 351
HpySE526I ACGT 2 cut(s) 317, 413
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 6 cut(s) 74, 109, 190, 202, 368, 440
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 3 cut(s) 302, 355, 451
Lsp1109I GCAGC 4 cut(s) 133, 207, 363, 508
LweI GCATC 1 cut(s) 207
MaeI CTAG 2 cut(s) 336, 399
MaeII ACGT 2 cut(s) 317, 413
MalI GATC 3 cut(s) 304, 357, 453
MboI GATC 3 cut(s) 302, 355, 451
MboII GAAGA 3 cut(s) 272, 473, 559
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 3 cut(s) 7, 137, 289
MlyI GAGTC 1 cut(s) 464
MnlI CCTC 4 cut(s) 98, 227, 300, 402
MseI TTAA 2 cut(s) 281, 540
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 6 cut(s) 39, 347, 360, 443, 458, 479
MspR9I CCNGG 3 cut(s) 347, 360, 361
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 229, 351
NciI CCSGG 3 cut(s) 347, 360, 361
NdeII GATC 3 cut(s) 302, 355, 451
NlaIII CATG 6 cut(s) 74, 109, 190, 202, 368, 440
NmeAIII GCCGAG 1 cut(s) 124
NspI RCATGY 2 cut(s) 368, 440
PaeI GCATGC 1 cut(s) 368
PaeR7I CTCGAG 2 cut(s) 110, 472
PfeI GAWTC 1 cut(s) 395
PflFI GACNNNGTC 1 cut(s) 415
PkrI GCNGC 4 cut(s) 123, 222, 378, 498
Ple19I CGATCG 1 cut(s) 305
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 105
Psp1406I AACGTT 1 cut(s) 317
PstI CTGCAG 1 cut(s) 184
PsyI GACNNNGTC 1 cut(s) 415
PvuI CGATCG 1 cut(s) 305
RsaI GTAC 1 cut(s) 514
RsaNI GTAC 1 cut(s) 513
SacI GAGCTC 1 cut(s) 105
SacII CCGCGG 1 cut(s) 271
SaqAI TTAA 2 cut(s) 281, 540
SatI GCNGC 4 cut(s) 122, 221, 377, 497
Sau3AI GATC 3 cut(s) 302, 355, 451
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 3 cut(s) 347, 360, 361
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 105, 195, 210, 311, 320, 328, 416, 426, 501
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 180
Sfr274I CTCGAG 2 cut(s) 110, 472
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
SlaI CTCGAG 2 cut(s) 110, 472
SmaI CCCGGG 1 cut(s) 361
SmlI CTYRAG 2 cut(s) 110, 472
SmoI CTYRAG 2 cut(s) 110, 472
SphI GCATGC 1 cut(s) 368
Sse9I AATT 3 cut(s) 7, 137, 289
SsiI CCGC 3 cut(s) 119, 268, 270
SspMI CTAG 2 cut(s) 336, 399
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 3 cut(s) 345, 358, 359
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 2 cut(s) 94, 246
TaiI ACGT 2 cut(s) 320, 416
TaqI TCGA 4 cut(s) 111, 195, 305, 473
TasI AATT 3 cut(s) 7, 137, 289
TatI WGTACW 1 cut(s) 512
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 281, 540
Tru9I TTAA 2 cut(s) 281, 540
TscAI CASTG 1 cut(s) 249
TseI GCWGC 4 cut(s) 121, 220, 376, 496
TspDTI ATGAA 3 cut(s) 203, 215, 242
TspMI CCCGGG 1 cut(s) 359
TspRI CASTG 1 cut(s) 249
Tth111I GACNNNGTC 1 cut(s) 415
XapI RAATTY 2 cut(s) 137, 289
XceI RCATGY 2 cut(s) 368, 440
XhoI CTCGAG 2 cut(s) 110, 472
XmaI CCCGGG 1 cut(s) 359
XspI CTAG 2 cut(s) 336, 399
ZraI GACGTC 1 cut(s) 414
ZrmI AGTACT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.