endonuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1811 |
| AatII | GACGTC | 1 cut(s) | 1733 |
| Acc36I | ACCTGC | 1 cut(s) | 1318 |
| AccB1I | GGYRCC | 1 cut(s) | 2323 |
| AccI | GTMKAC | 1 cut(s) | 1803 |
| AciI | CCGC | 3 cut(s) | 1169, 1560, 1931 |
| AclWI | GGATC | 3 cut(s) | 1516, 1693, 2157 |
| AcoI | YGGCCR | 1 cut(s) | 2222 |
| AcsI | RAATTY | 5 cut(s) | 971, 1060, 1178, 1754, 2254 |
| AcuI | CTGAAG | 9 cut(s) | 330, 486, 501, 570, 705, 1098, 1242, 1263, 2229 |
| AcyI | GRCGYC | 1 cut(s) | 1730 |
| AfaI | GTAC | 2 cut(s) | 58, 400 |
| AfeI | AGCGCT | 2 cut(s) | 1576, 1999 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 38, 394, 395, 1988, 2213 |
| AflII | CTTAAG | 1 cut(s) | 1084 |
| AflIII | ACRYGT | 1 cut(s) | 2086 |
| AhlI | ACTAGT | 1 cut(s) | 794 |
| AjiI | CACGTC | 1 cut(s) | 2089 |
| AjnI | CCWGG | 1 cut(s) | 31 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2302, 2334 |
| Alw21I | GWGCWC | 2 cut(s) | 115, 538 |
| Alw26I | GTCTC | 5 cut(s) | 365, 638, 1004, 1125, 2192 |
| Alw44I | GTGCAC | 1 cut(s) | 534 |
| AlwI | GGATC | 3 cut(s) | 1516, 1693, 2157 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1697 |
| Aor51HI | AGCGCT | 2 cut(s) | 1576, 1999 |
| AoxI | GGCC | 3 cut(s) | 444, 1150, 2222 |
| ApaLI | GTGCAC | 1 cut(s) | 534 |
| ApeKI | GCWGC | 4 cut(s) | 21, 281, 1358, 2337 |
| ApoI | RAATTY | 5 cut(s) | 971, 1060, 1178, 1754, 2254 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 471 |
| AspLEI | GCGC | 2 cut(s) | 1577, 2000 |
| AspS9I | GGNCC | 4 cut(s) | 13, 767, 1840, 1979 |
| AsuC2I | CCSGG | 2 cut(s) | 389, 411 |
| AsuHPI | GGTGA | 5 cut(s) | 425, 629, 2035, 2115, 2250 |
| AsuNHI | GCTAGC | 1 cut(s) | 818 |
| AvaII | GGWCC | 4 cut(s) | 13, 767, 1840, 1979 |
| BaeGI | GKGCMC | 2 cut(s) | 538, 2072 |
| BalI | TGGCCA | 1 cut(s) | 2224 |
| BanI | GGYRCC | 1 cut(s) | 2323 |
| BauI | CACGAG | 1 cut(s) | 2153 |
| BbsI | GAAGAC | 3 cut(s) | 2025, 2060, 2317 |
| Bbv12I | GWGCWC | 2 cut(s) | 115, 538 |
| BbvCI | CCTCAGC | 1 cut(s) | 1836 |
| BbvI | GCAGC | 4 cut(s) | 33, 268, 1370, 2324 |
| BccI | CCATC | 2 cut(s) | 143, 2207 |
| BceAI | ACGGC | 1 cut(s) | 459 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 239, 263, 273, 297, 1148, 1182 |
| BciT130I | CCWGG | 1 cut(s) | 33 |
| BciVI | GTATCC | 2 cut(s) | 709, 2280 |
| BclI | TGATCA | 1 cut(s) | 1522 |
| BcnI | CCSGG | 2 cut(s) | 389, 411 |
| BcoDI | GTCTC | 5 cut(s) | 365, 638, 1004, 1125, 2192 |
| BcuI | ACTAGT | 1 cut(s) | 794 |
| BfaI | CTAG | 6 cut(s) | 93, 795, 819, 1926, 1934, 1941 |
| BfmI | CTRYAG | 3 cut(s) | 19, 1314, 1323 |
| BfoI | RGCGCY | 2 cut(s) | 1578, 2001 |
| BfrI | CTTAAG | 1 cut(s) | 1084 |
| BfuAI | ACCTGC | 1 cut(s) | 1318 |
| BfuI | GTATCC | 2 cut(s) | 709, 2280 |
| BglII | AGATCT | 3 cut(s) | 150, 350, 1913 |
| BisI | GCNGC | 4 cut(s) | 22, 282, 1359, 2338 |
| BlsI | GCNGC | 4 cut(s) | 23, 283, 1360, 2339 |
| Bme1390I | CCNGG | 3 cut(s) | 33, 389, 411 |
| Bme18I | GGWCC | 4 cut(s) | 13, 767, 1840, 1979 |
| BmgBI | CACGTC | 1 cut(s) | 2089 |
| BmgT120I | GGNCC | 4 cut(s) | 13, 767, 1840, 1979 |
| BmiI | GGNNCC | 6 cut(s) | 133, 768, 769, 1842, 1980, 2325 |
| BmrFI | CCNGG | 3 cut(s) | 33, 389, 411 |
| BmrI | ACTGGG | 3 cut(s) | 1179, 1838, 2198 |
| BmsI | GCATC | 5 cut(s) | 319, 701, 862, 1504, 2033 |
| BmtI | GCTAGC | 1 cut(s) | 822 |
| BmuI | ACTGGG | 3 cut(s) | 1179, 1838, 2198 |
| BpiI | GAAGAC | 3 cut(s) | 2025, 2060, 2317 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1288, 1320 |
| BpmI | CTGGAG | 4 cut(s) | 15, 843, 1149, 2022 |
| Bpu10I | CCTNAGC | 2 cut(s) | 600, 1836 |
| BpuEI | CTTGAG | 2 cut(s) | 1131, 1315 |
| BpuMI | CCSGG | 2 cut(s) | 389, 411 |
| BsaHI | GRCGYC | 1 cut(s) | 1730 |
| BsaJI | CCNNGG | 2 cut(s) | 2046, 2126 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 38, 394, 395, 1988, 2213 |
| BseBI | CCWGG | 1 cut(s) | 33 |
| BseDI | CCNNGG | 2 cut(s) | 2046, 2126 |
| BseGI | GGATG | 8 cut(s) | 88, 214, 307, 322, 805, 844, 1547, 2201 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 38, 394, 395, 1988, 2213 |
| BseRI | GAGGAG | 2 cut(s) | 51, 2306 |
| BseSI | GKGCMC | 2 cut(s) | 538, 2072 |
| BseXI | GCAGC | 4 cut(s) | 33, 268, 1370, 2324 |
| BseYI | CCCAGC | 1 cut(s) | 2064 |
| BsgI | GTGCAG | 3 cut(s) | 1209, 1377, 1683 |
| BshFI | GGCC | 3 cut(s) | 446, 1152, 2224 |
| BshNI | GGYRCC | 1 cut(s) | 2323 |
| BsiHKAI | GWGCWC | 2 cut(s) | 115, 538 |
| BsiSI | CCGG | 3 cut(s) | 191, 389, 411 |
| BslFI | GGGAC | 5 cut(s) | 753, 1826, 1992, 2075, 2205 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 38, 394, 395, 1988, 2213 |
| BsmAI | GTCTC | 5 cut(s) | 365, 638, 1004, 1125, 2192 |
| BsmFI | GGGAC | 5 cut(s) | 753, 1826, 1992, 2075, 2205 |
| BsmI | GAATGC | 4 cut(s) | 541, 656, 937, 1273 |
| BsnI | GGCC | 3 cut(s) | 446, 1152, 2224 |
| Bsp1286I | GDGCHC | 3 cut(s) | 115, 538, 2072 |
| BspACI | CCGC | 3 cut(s) | 1169, 1560, 1931 |
| BspANI | GGCC | 3 cut(s) | 446, 1152, 2224 |
| BspLI | GGNNCC | 6 cut(s) | 133, 768, 769, 1842, 1980, 2325 |
| BspMAI | CTGCAG | 1 cut(s) | 23 |
| BspMI | ACCTGC | 1 cut(s) | 1318 |
| BspOI | GCTAGC | 1 cut(s) | 822 |
| BspPI | GGATC | 3 cut(s) | 1516, 1693, 2157 |
| BspT107I | GGYRCC | 1 cut(s) | 2323 |
| BspTI | CTTAAG | 1 cut(s) | 1084 |
| BssECI | CCNNGG | 2 cut(s) | 2046, 2126 |
| BssNI | GRCGYC | 1 cut(s) | 1730 |
| BssSI | CACGAG | 1 cut(s) | 2153 |
| BssT1I | CCWWGG | 2 cut(s) | 2046, 2126 |
| Bst2BI | CACGAG | 1 cut(s) | 2153 |
| Bst2UI | CCWGG | 1 cut(s) | 33 |
| Bst4CI | ACNGT | 8 cut(s) | 107, 244, 268, 577, 614, 1279, 1711, 1808 |
| Bst6I | CTCTTC | 3 cut(s) | 306, 363, 1239 |
| BstACI | GRCGYC | 1 cut(s) | 1730 |
| BstAFI | CTTAAG | 1 cut(s) | 1084 |
| BstC8I | GCNNGC | 2 cut(s) | 820, 1413 |
| BstF5I | GGATG | 8 cut(s) | 88, 214, 307, 322, 805, 844, 1547, 2201 |
| BstH2I | RGCGCY | 2 cut(s) | 1578, 2001 |
| BstHHI | GCGC | 2 cut(s) | 1577, 2000 |
| BstMAI | GTCTC | 5 cut(s) | 365, 638, 1004, 1125, 2192 |
| BstNI | CCWGG | 1 cut(s) | 33 |
| BstSCI | CCNGG | 3 cut(s) | 31, 387, 409 |
| BstSFI | CTRYAG | 3 cut(s) | 19, 1314, 1323 |
| BstSLI | GKGCMC | 2 cut(s) | 538, 2072 |
| BstV1I | GCAGC | 4 cut(s) | 33, 268, 1370, 2324 |
| BstV2I | GAAGAC | 3 cut(s) | 2025, 2060, 2317 |
| BstX2I | RGATCY | 4 cut(s) | 150, 350, 1508, 1913 |
| BstYI | RGATCY | 4 cut(s) | 150, 350, 1508, 1913 |
| BsuI | GTATCC | 2 cut(s) | 709, 2280 |
| BsuRI | GGCC | 3 cut(s) | 446, 1152, 2224 |
| BtrI | CACGTC | 1 cut(s) | 2089 |
| BtsCI | GGATG | 8 cut(s) | 88, 214, 307, 322, 805, 844, 1547, 2201 |
| BtsI | GCAGTG | 4 cut(s) | 16, 431, 1360, 1695 |
| BveI | ACCTGC | 1 cut(s) | 1318 |
| Cac8I | GCNNGC | 2 cut(s) | 820, 1413 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1697 |
| CfoI | GCGC | 2 cut(s) | 1577, 2000 |
| Cfr13I | GGNCC | 4 cut(s) | 13, 767, 1840, 1979 |
| CseI | GACGC | 1 cut(s) | 2030 |
| Csp6I | GTAC | 2 cut(s) | 57, 399 |
| CviAII | CATG | 2 cut(s) | 50, 950 |
| CviQI | GTAC | 2 cut(s) | 57, 399 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1811 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1811 |
| EaeI | YGGCCR | 1 cut(s) | 2222 |
| Eam1104I | CTCTTC | 3 cut(s) | 306, 363, 1239 |
| EarI | CTCTTC | 3 cut(s) | 306, 363, 1239 |
| EciI | GGCGGA | 1 cut(s) | 1549 |
| Eco130I | CCWWGG | 2 cut(s) | 2046, 2126 |
| Eco32I | GATATC | 2 cut(s) | 1206, 1228 |
| Eco47I | GGWCC | 4 cut(s) | 13, 767, 1840, 1979 |
| Eco47III | AGCGCT | 2 cut(s) | 1576, 1999 |
| Eco57I | CTGAAG | 9 cut(s) | 330, 486, 501, 570, 705, 1098, 1242, 1263, 2229 |
| EcoO109I | RGGNCCY | 2 cut(s) | 767, 1840 |
| EcoRI | GAATTC | 2 cut(s) | 1754, 2254 |
| EcoRII | CCWGG | 1 cut(s) | 31 |
| EcoRV | GATATC | 2 cut(s) | 1206, 1228 |
| EcoT14I | CCWWGG | 2 cut(s) | 2046, 2126 |
| ErhI | CCWWGG | 2 cut(s) | 2046, 2126 |
| FaeI | CATG | 2 cut(s) | 53, 953 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 165, 197 |
| FaqI | GGGAC | 5 cut(s) | 753, 1826, 1992, 2075, 2205 |
| FatI | CATG | 2 cut(s) | 49, 949 |
| FbaI | TGATCA | 1 cut(s) | 1522 |
| FblI | GTMKAC | 1 cut(s) | 1803 |
| Fnu4HI | GCNGC | 4 cut(s) | 22, 282, 1359, 2338 |
| FokI | GGATG | 8 cut(s) | 75, 221, 314, 329, 792, 851, 1534, 2188 |
| Fsp4HI | GCNGC | 4 cut(s) | 22, 282, 1359, 2338 |
| FspBI | CTAG | 6 cut(s) | 93, 795, 819, 1926, 1934, 1941 |
| GlaI | GCGC | 2 cut(s) | 1576, 1999 |
| GluI | GCNGC | 4 cut(s) | 22, 282, 1359, 2338 |
| GsaI | CCCAGC | 1 cut(s) | 2068 |
| GsuI | CTGGAG | 4 cut(s) | 15, 843, 1149, 2022 |
| HaeII | RGCGCY | 2 cut(s) | 1578, 2001 |
| HaeIII | GGCC | 3 cut(s) | 446, 1152, 2224 |
| HapII | CCGG | 3 cut(s) | 191, 389, 411 |
| HgaI | GACGC | 1 cut(s) | 2030 |
| HhaI | GCGC | 2 cut(s) | 1577, 2000 |
| Hin1I | GRCGYC | 1 cut(s) | 1730 |
| Hin1II | CATG | 2 cut(s) | 53, 953 |
| Hin6I | GCGC | 2 cut(s) | 1575, 1998 |
| HinP1I | GCGC | 2 cut(s) | 1575, 1998 |
| HincII | GTYRAC | 4 cut(s) | 666, 1333, 1804, 2008 |
| HindII | GTYRAC | 4 cut(s) | 666, 1333, 1804, 2008 |
| HindIII | AAGCTT | 1 cut(s) | 1118 |
| HpaI | GTTAAC | 2 cut(s) | 666, 2008 |
| HpaII | CCGG | 3 cut(s) | 191, 389, 411 |
| HphI | GGTGA | 5 cut(s) | 425, 629, 2035, 2115, 2250 |
| Hpy166II | GTNNAC | 8 cut(s) | 324, 536, 666, 1333, 1403, 1804, 2008, 2278 |
| Hpy188III | TCNNGA | 9 cut(s) | 11, 182, 348, 476, 809, 908, 1007, 1871, 2153 |
| Hpy8I | GTNNAC | 8 cut(s) | 324, 536, 666, 1333, 1403, 1804, 2008, 2278 |
| Hpy99I | CGWCG | 2 cut(s) | 111, 1808 |
| HpyAV | CCTTC | 6 cut(s) | 545, 1073, 1426, 1611, 1740, 2114 |
| HpyCH4III | ACNGT | 8 cut(s) | 107, 244, 268, 577, 614, 1279, 1711, 1808 |
| HpyCH4IV | ACGT | 2 cut(s) | 1730, 2088 |
| HpySE526I | ACGT | 2 cut(s) | 1730, 2088 |
| Hsp92I | GRCGYC | 1 cut(s) | 1730 |
| Hsp92II | CATG | 2 cut(s) | 53, 953 |
| HspAI | GCGC | 2 cut(s) | 1575, 1998 |
| KflI | GGGWCCC | 1 cut(s) | 767 |
| Ksp22I | TGATCA | 1 cut(s) | 1522 |
| KspAI | GTTAAC | 2 cut(s) | 666, 2008 |
| LmnI | GCTCC | 3 cut(s) | 265, 2080, 2345 |
| Lsp1109I | GCAGC | 4 cut(s) | 33, 268, 1370, 2324 |
| LweI | GCATC | 5 cut(s) | 319, 701, 862, 1504, 2033 |
| MaeI | CTAG | 6 cut(s) | 93, 795, 819, 1926, 1934, 1941 |
| MaeII | ACGT | 2 cut(s) | 1730, 2088 |
| MaeIII | GTNAC | 6 cut(s) | 1607, 1731, 1846, 1936, 2159, 2216 |
| MflI | RGATCY | 4 cut(s) | 150, 350, 1508, 1913 |
| MhlI | GDGCHC | 3 cut(s) | 115, 538, 2072 |
| MlsI | TGGCCA | 1 cut(s) | 2224 |
| MluNI | TGGCCA | 1 cut(s) | 2224 |
| MlyI | GAGTC | 6 cut(s) | 152, 593, 796, 991, 1328, 1550 |
| MmeI | TCCRAC | 3 cut(s) | 50, 263, 287 |
| Mox20I | TGGCCA | 1 cut(s) | 2224 |
| MroXI | GAANNNNTTC | 1 cut(s) | 471 |
| MscI | TGGCCA | 1 cut(s) | 2224 |
| MseI | TTAA | 8 cut(s) | 665, 1071, 1085, 1215, 1713, 2007, 2282, 2349 |
| MslI | CAYNNNNRTG | 2 cut(s) | 93, 810 |
| Msp20I | TGGCCA | 1 cut(s) | 2224 |
| MspA1I | CMGCKG | 1 cut(s) | 483 |
| MspCI | CTTAAG | 1 cut(s) | 1084 |
| MspI | CCGG | 3 cut(s) | 191, 389, 411 |
| MspR9I | CCNGG | 3 cut(s) | 33, 389, 411 |
| Mva1269I | GAATGC | 4 cut(s) | 541, 656, 937, 1273 |
| MvaI | CCWGG | 1 cut(s) | 33 |
| NciI | CCSGG | 2 cut(s) | 389, 411 |
| NheI | GCTAGC | 1 cut(s) | 818 |
| NlaIII | CATG | 2 cut(s) | 53, 953 |
| NlaIV | GGNNCC | 6 cut(s) | 133, 768, 769, 1842, 1980, 2325 |
| NmuCI | GTSAC | 4 cut(s) | 1731, 1846, 1936, 2216 |
| PctI | GAATGC | 4 cut(s) | 541, 656, 937, 1273 |
| PdmI | GAANNNNTTC | 1 cut(s) | 471 |
| PfoI | TCCNGGA | 1 cut(s) | 31 |
| PkrI | GCNGC | 4 cut(s) | 23, 283, 1360, 2339 |
| PleI | GAGTC | 6 cut(s) | 152, 592, 796, 990, 1328, 1550 |
| PpsI | GAGTC | 6 cut(s) | 152, 592, 796, 990, 1328, 1550 |
| PpuMI | RGGWCCY | 2 cut(s) | 767, 1840 |
| Psp5II | RGGWCCY | 2 cut(s) | 767, 1840 |
| Psp6I | CCWGG | 1 cut(s) | 31 |
| PspFI | CCCAGC | 1 cut(s) | 2064 |
| PspGI | CCWGG | 1 cut(s) | 31 |
| PspN4I | GGNNCC | 6 cut(s) | 133, 768, 769, 1842, 1980, 2325 |
| PspPI | GGNCC | 4 cut(s) | 13, 767, 1840, 1979 |
| PspPPI | RGGWCCY | 2 cut(s) | 767, 1840 |
| PstI | CTGCAG | 1 cut(s) | 23 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1697 |
| PsuI | RGATCY | 4 cut(s) | 150, 350, 1508, 1913 |
| PvuII | CAGCTG | 1 cut(s) | 483 |
| RsaI | GTAC | 2 cut(s) | 58, 400 |
| RsaNI | GTAC | 2 cut(s) | 57, 399 |
| RseI | CAYNNNNRTG | 2 cut(s) | 93, 810 |
| SalI | GTCGAC | 1 cut(s) | 1802 |
| SaqAI | TTAA | 8 cut(s) | 665, 1071, 1085, 1215, 1713, 2007, 2282, 2349 |
| SatI | GCNGC | 4 cut(s) | 22, 282, 1359, 2338 |
| Sau96I | GGNCC | 4 cut(s) | 13, 767, 1840, 1979 |
| SchI | GAGTC | 6 cut(s) | 152, 593, 796, 991, 1328, 1550 |
| ScrFI | CCNGG | 3 cut(s) | 33, 389, 411 |
| SduI | GDGCHC | 3 cut(s) | 115, 538, 2072 |
| SfaNI | GCATC | 5 cut(s) | 319, 701, 862, 1504, 2033 |
| SfcI | CTRYAG | 3 cut(s) | 19, 1314, 1323 |
| SinI | GGWCC | 4 cut(s) | 13, 767, 1840, 1979 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 93, 810 |
| SmlI | CTYRAG | 3 cut(s) | 1084, 1146, 1294 |
| SmoI | CTYRAG | 3 cut(s) | 1084, 1146, 1294 |
| SpeI | ACTAGT | 1 cut(s) | 794 |
| SsiI | CCGC | 3 cut(s) | 1169, 1560, 1931 |
| SspMI | CTAG | 6 cut(s) | 93, 795, 819, 1926, 1934, 1941 |
| StyD4I | CCNGG | 3 cut(s) | 31, 387, 409 |
| StyI | CCWWGG | 2 cut(s) | 2046, 2126 |
| TaaI | ACNGT | 8 cut(s) | 107, 244, 268, 577, 614, 1279, 1711, 1808 |
| TaiI | ACGT | 2 cut(s) | 1733, 2091 |
| TaqI | TCGA | 7 cut(s) | 249, 273, 347, 526, 1803, 1870, 2183 |
| TatI | WGTACW | 1 cut(s) | 56 |
| Tru1I | TTAA | 8 cut(s) | 665, 1071, 1085, 1215, 1713, 2007, 2282, 2349 |
| Tru9I | TTAA | 8 cut(s) | 665, 1071, 1085, 1215, 1713, 2007, 2282, 2349 |
| TseFI | GTSAC | 4 cut(s) | 1731, 1846, 1936, 2216 |
| TseI | GCWGC | 4 cut(s) | 21, 281, 1358, 2337 |
| Tsp45I | GTSAC | 4 cut(s) | 1731, 1846, 1936, 2216 |
| TspGWI | ACGGA | 2 cut(s) | 1629, 1676 |
| Vha464I | CTTAAG | 1 cut(s) | 1084 |
| VneI | GTGCAC | 1 cut(s) | 534 |
| VpaK11BI | GGWCC | 4 cut(s) | 13, 767, 1840, 1979 |
| XapI | RAATTY | 5 cut(s) | 971, 1060, 1178, 1754, 2254 |
| XmiI | GTMKAC | 1 cut(s) | 1803 |
| XmnI | GAANNNNTTC | 1 cut(s) | 471 |
| XspI | CTAG | 6 cut(s) | 93, 795, 819, 1926, 1934, 1941 |
| ZraI | GACGTC | 1 cut(s) | 1731 |