Rh4CG184800

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001352
Physical Location & Seq
Reverse (-)
39803103 .. 39803918
816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG184800.1

Sequence Viewer

Length: 489 bp
ATGGATCACCAAGATCTCAGGAGAGCCCACAGTTCAGGGCACGAGAGTCACCATGTGTGTCACAAATGCGGGTGGCCTTTTCCGAATCCGCACCCGAGTGCTAGACACAGACGCGCGCATAAGAAGATTTGTGGAACCGTTGAGGGTTACAAGGTGGCTGGCGCCGAGGGGAGTGCACAGTTGAGTGTTTCTGATGATGAGCAGCACTCTGATGAAGATCCCAAAACCCCCGGTCCGAAAGTCTTGGAAAGGAGCGTTTATGAGAAGGGTAGTGGCGGAATTGGGCAAGGATCTATTAAATCAGATGATGGGGTGTTCTCAGATGCTGTTCAGGAGTTTTCAGATAGTGGTCCTGCTGCATGGACCGGAGAAGGGTTGGAGGATGCCCCGGGATCAGCACCTAATGTAGAAAGGGGTGCCAAGAGTGATTTGAATGCCATTCCGTTCCACAATGATGGGGGAACGACAGGTAACCTTCCAATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.19

Weight (kDa)

5.83

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 161, 416
AccII CGCG 2 cut(s) 114, 116
AciI CCGC 3 cut(s) 69, 89, 276
AclWI GGATC 4 cut(s) 12, 212, 298, 400
AcyI GRCGYC 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 372
AgsI TTSAA 1 cut(s) 433
AleI CACNNNNGTG 1 cut(s) 96
Alw21I GWGCWC 1 cut(s) 178
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 4 cut(s) 12, 212, 298, 400
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 2 cut(s) 94, 388
AoxI GGCC 1 cut(s) 74
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 2 cut(s) 202, 356
AspLEI GCGC 3 cut(s) 116, 118, 164
AspS9I GGNCC 3 cut(s) 233, 350, 363
AsuC2I CCSGG 3 cut(s) 231, 389, 390
AsuHPI GGTGA 1 cut(s) 41
AvaI CYCGRG 2 cut(s) 94, 388
AvaII GGWCC 3 cut(s) 233, 350, 363
BaeGI GKGCMC 2 cut(s) 42, 178
BanI GGYRCC 2 cut(s) 161, 416
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 2 cut(s) 214, 343
BccI CCATC 2 cut(s) 302, 449
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcnI CCSGG 3 cut(s) 231, 389, 390
BfaI CTAG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 165
BglII AGATCT 1 cut(s) 13
BisI GCNGC 2 cut(s) 203, 357
BlsI GCNGC 2 cut(s) 204, 358
Bme1390I CCNGG 3 cut(s) 231, 389, 390
Bme18I GGWCC 3 cut(s) 233, 350, 363
BmeT110I CYCGRG 2 cut(s) 94, 388
BmgT120I GGNCC 3 cut(s) 233, 350, 363
BmiI GGNNCC 3 cut(s) 136, 163, 418
BmrFI CCNGG 3 cut(s) 231, 389, 390
BmsI GCATC 2 cut(s) 313, 373
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpuMI CCSGG 3 cut(s) 231, 389, 390
BsaBI GATNNNNATC 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 4 cut(s) 165, 229, 387, 388
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 216
BseDI CCNNGG 4 cut(s) 165, 229, 387, 388
BseGI GGATG 1 cut(s) 388
BseJI GATNNNNATC 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 372
BseMII CTCAG 2 cut(s) 31, 333
BsePI GCGCGC 1 cut(s) 114
BseSI GKGCMC 2 cut(s) 42, 178
BseXI GCAGC 2 cut(s) 214, 343
Bsh1236I CGCG 2 cut(s) 114, 116
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 2 cut(s) 161, 416
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 2 cut(s) 94, 388
BsiSI CCGG 3 cut(s) 231, 366, 389
BslI CCNNNNNNNGG 1 cut(s) 372
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 1 cut(s) 76
BsoBI CYCGRG 2 cut(s) 94, 388
Bsp1286I GDGCHC 3 cut(s) 28, 42, 178
Bsp143I GATC 5 cut(s) 4, 13, 217, 290, 392
BspACI CCGC 3 cut(s) 69, 89, 276
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 2 cut(s) 30, 332
BspFNI CGCG 2 cut(s) 114, 116
BspLI GGNNCC 3 cut(s) 136, 163, 418
BspPI GGATC 4 cut(s) 12, 212, 298, 400
BspT107I GGYRCC 2 cut(s) 161, 416
BssECI CCNNGG 4 cut(s) 165, 229, 387, 388
BssHII GCGCGC 1 cut(s) 114
BssMI GATC 5 cut(s) 4, 13, 217, 290, 392
BssNI GRCGYC 1 cut(s) 162
BssSI CACGAG 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 41
Bst4CI ACNGT 3 cut(s) 32, 139, 180
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 116, 160
BstDEI CTNAG 2 cut(s) 17, 319
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 2 cut(s) 114, 116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 3 cut(s) 116, 118, 164
BstKTI GATC 5 cut(s) 7, 16, 220, 293, 395
BstMBI GATC 5 cut(s) 4, 13, 217, 290, 392
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 229, 387, 388
BstSLI GKGCMC 2 cut(s) 42, 178
BstUI CGCG 2 cut(s) 114, 116
BstV1I GCAGC 2 cut(s) 214, 343
BstX2I RGATCY 3 cut(s) 13, 217, 290
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 3 cut(s) 13, 217, 290
BsuRI GGCC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 388
Cac8I GCNNGC 2 cut(s) 116, 160
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 3 cut(s) 116, 118, 164
Cfr13I GGNCC 3 cut(s) 233, 350, 363
Cfr9I CCCGGG 1 cut(s) 388
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 1 cut(s) 120
CspI CGGWCCG 1 cut(s) 233
CviAII CATG 2 cut(s) 53, 360
CviJI RGCY 3 cut(s) 26, 76, 158
CviKI_1 RGCY 3 cut(s) 26, 76, 158
DdeI CTNAG 2 cut(s) 17, 319
DinI GGCGCC 1 cut(s) 163
DpnI GATC 5 cut(s) 6, 15, 219, 292, 394
DpnII GATC 5 cut(s) 4, 13, 217, 290, 392
DraIII CACNNNGTG 1 cut(s) 55
EciI GGCGGA 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 3 cut(s) 233, 350, 363
Eco88I CYCGRG 2 cut(s) 94, 388
Eco91I GGTNACC 1 cut(s) 470
EcoO65I GGTNACC 1 cut(s) 470
EcoT38I GRGCYC 1 cut(s) 28
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 2 cut(s) 56, 363
FaiI YATR 4 cut(s) 54, 120, 261, 361
FatI CATG 2 cut(s) 52, 359
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 2 cut(s) 203, 357
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 2 cut(s) 203, 357
FspBI CTAG 1 cut(s) 102
GlaI GCGC 3 cut(s) 115, 117, 163
GluI GCNGC 2 cut(s) 203, 357
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 1 cut(s) 76
HapII CCGG 3 cut(s) 231, 366, 389
HgaI GACGC 1 cut(s) 120
HhaI GCGC 3 cut(s) 116, 118, 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 2 cut(s) 56, 363
Hin6I GCGC 3 cut(s) 114, 116, 162
HinP1I GCGC 3 cut(s) 114, 116, 162
HinfI GANTC 2 cut(s) 46, 85
HpaII CCGG 3 cut(s) 231, 366, 389
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 176
Hpy188I TCNGA 7 cut(s) 84, 193, 211, 237, 304, 322, 343
Hpy188III TCNNGA 2 cut(s) 19, 332
Hpy8I GTNNAC 1 cut(s) 176
HpyAV CCTTC 3 cut(s) 259, 365, 485
HpyCH4III ACNGT 3 cut(s) 32, 139, 180
HpyCH4V TGCA 2 cut(s) 176, 359
HpyF3I CTNAG 2 cut(s) 17, 319
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 2 cut(s) 56, 363
HspAI GCGC 3 cut(s) 114, 116, 162
KasI GGCGCC 1 cut(s) 161
Kzo9I GATC 5 cut(s) 4, 13, 217, 290, 392
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 9 cut(s) 4, 21, 144, 244, 317, 366, 379, 402, 453
Lsp1109I GCAGC 2 cut(s) 214, 343
LweI GCATC 2 cut(s) 313, 373
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 4 cut(s) 47, 59, 146, 470
MalI GATC 5 cut(s) 6, 15, 219, 292, 394
MboI GATC 5 cut(s) 4, 13, 217, 290, 392
MboII GAAGA 2 cut(s) 136, 227
MflI RGATCY 3 cut(s) 13, 217, 290
MhlI GDGCHC 3 cut(s) 28, 42, 178
MluCI AATT 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 3 cut(s) 136, 160, 373
MseI TTAA 1 cut(s) 297
MslI CAYNNNNRTG 3 cut(s) 96, 210, 453
MspI CCGG 3 cut(s) 231, 366, 389
MspR9I CCNGG 3 cut(s) 231, 389, 390
Mva1269I GAATGC 1 cut(s) 439
MvnI CGCG 2 cut(s) 114, 116
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 3 cut(s) 231, 389, 390
NdeII GATC 5 cut(s) 4, 13, 217, 290, 392
NlaIII CATG 2 cut(s) 56, 363
NlaIV GGNNCC 3 cut(s) 136, 163, 418
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 47, 59
OliI CACNNNNGTG 1 cut(s) 96
PauI GCGCGC 1 cut(s) 114
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 2 cut(s) 204, 358
PleI GAGTC 1 cut(s) 54
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 54
PspEI GGTNACC 1 cut(s) 470
PspN4I GGNNCC 3 cut(s) 136, 163, 418
PspPI GGNCC 3 cut(s) 233, 350, 363
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 3 cut(s) 13, 217, 290
PteI GCGCGC 1 cut(s) 114
RseI CAYNNNNRTG 3 cut(s) 96, 210, 453
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 2 cut(s) 203, 357
Sau3AI GATC 5 cut(s) 4, 13, 217, 290, 392
Sau96I GGNCC 3 cut(s) 233, 350, 363
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 3 cut(s) 231, 389, 390
SduI GDGCHC 3 cut(s) 28, 42, 178
SetI ASST 4 cut(s) 156, 403, 472, 477
SfaNI GCATC 2 cut(s) 313, 373
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 3 cut(s) 233, 350, 363
SmaI CCCGGG 1 cut(s) 390
SmiMI CAYNNNNRTG 3 cut(s) 96, 210, 453
Sse9I AATT 1 cut(s) 279
SsiI CCGC 3 cut(s) 69, 89, 276
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 229, 387, 388
TaaI ACNGT 3 cut(s) 32, 139, 180
TasI AATT 1 cut(s) 279
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TseFI GTSAC 2 cut(s) 47, 59
TseI GCWGC 2 cut(s) 202, 356
Tsp45I GTSAC 2 cut(s) 47, 59
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 432
TspMI CCCGGG 1 cut(s) 388
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 3 cut(s) 233, 350, 363
XmaI CCCGGG 1 cut(s) 388
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.