RchiOBHm_Chr4g0412531

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001352
Physical Location & Seq
Reverse (-)
36345935 .. 36352149
6215 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38320

Sequence Viewer

Length: 3909 bp
ATGGATCACCAAGATCTCAGGCGAGCCCACAGTTCAGGGCACGAGAGTCACCATGTGTGTCACAAATGCGGGTGGCCTTTTCCGAATCCGCACCCGAGTGCTAGACACAGACGCGCGCATAAGAAGATTTGCGGAACCGTTGAGGGTTATAAGGTGGCTGGCGCCGAGGGGAGTGCACAGTTGAGTGTCTCTGATGATGAGCAGCACTCTGATGAGGATCCCAAAACCCCCGGTCCGAAAGTCGTGGAAAGGAGCGTTTATGAGAGGGGTAGTGGCGGAATCGGGCAAGGATCTATTAAATCAGATGATGGGGTGTTCTCAGATGCTGTTCAAGAGTTTTCAGATAGCGGTTCTGCTGCATGGACCGGAGAAGGGTTGGAGGATGCCCCGGGATCAGCACGTAATGTAGAAAGGGGTGCCAAGAGTGATTTGAATGCCATTCCGTTCCACAATGATGGGGGAACGACAGAAGACAAATCAAACCCCAATAAATATGAAATGGTGACAGATGCATCAGAAGATATGAAGAAGACCGATGTGACTGACAGCATGATTGAGAGCTGCTTAACATCTGTTGACCAGGAAGCTGAAGTGACAGGAATTACAGCAACTAATCTGGATAGGAACATCAGTTCAGGGACTACTTCTATTTCAGATGGTAGAACTGAAGAATCAAATGTGCTTCTTGGTGATAGCAATGGTTTACACCCCATTAAATATGAAATGGTGACAGATGCATCAGACGCTATTAAAAAGACTGGTGCCACTGACAGCATGATTGAGAGCTGCTTAACATCTGTTGATCAGGATGCTGAAGTGACGGGAATTACAGTAACTAATCTAGTTAGGAACATGATCTTACCCAATAAAGTTGCTGGTGAAACAGTTGAGGGTGGCTCTAAGTTAGAAGGATCAAATGAAATGACTTCAGAATATGTGCGAGATGATGAACTTGTTCCATCAGAGAAGGAGCATACTGATGGGTTTGATATGCATATTCCACAAATTGATGTCCCACCTGAAGTAGAATCTGTTGCACATGTGAATACTTTTGTTGATACTGCTGAAAAGAAGGTGGATAATACAGATGGAATACAGTATCTCAGTCCTGATAAAGTGATAGAGAGTCGTAATGGGAAGGAGGAAGAGAATGCAAATGCACATGTACATGTGCTTTCTGTGCCTGATGACATACATGTGGTAGATGATCCTGTGCTGATGCTTGAAGATTTCAGAAACCATAAAGGAAAGCAATTAGACCAAGTTACTTTCTTGGACTCATTGGAACAGTTTGATGATAAGGAAAATGATGTCAAACAACCTGTTTTCAACGAGAGTCTGAACATTTTCCAGTCAAGTCATCTAAGTGGAGGCAGTGTTGTCTCTTCTGATATGCATGATTTGGAAGCTAGCCTTGAACCGGATCTCGGCAGCAGCAAGCCAATGATTAAAAGTGTGCCTGTTGAAGATGAAGCCAATATGTCTGAGACCGAGATCAAGATGTTTAAAAACCAAAGTTCAGATGAAATAGGAGCCCATGCAGATGCTGTGATGCCTCAGATAGCTAAAACCTATATGGTGAACTTTGCTGCCAGTGATGCTGGTATAGGTCAAACAACCCACTCAGTTGGTCCAGAGAATAATGAAAGAGATGGAAACTGTGATGGGACTGGAGAGTCTGGAGACCACAGCAGAAAAGTAACTGCAGAAGAAAATCTTGTGTCAAATCCTATAGCTACTGTTCAATCTGCTGAAGGACAGGAATCTTATGAGAAGTCCAATGAGTCCGGTCAGGAATCTCATAATCTTATGAGAAACTGTGACGGGAGTGGAGAGTCTGGAGACCACAGCAGAAAAGTAACTGCAGAAGAAAATCTTGTGGCAAATCCTATAGCTACTGTTCAATCTGCTGAAGGACAGGTGTCTTATGAGAAGTCCAATGAGTCTTTACAGGTGAACCTTGCTGCTGGTGATGCTGGTATAGATGAAACCACCCACTCAGTTGGTCCAGTCGATAATGAAAAAGATGGAAACTGTGATGGGAGTGGAGAATCTGGAGACTACAGCAGAAAAGGACCCGCAGAAGTAAATCTTGTGGCAACTCCTATAGGTACTGTTCAATCAGCTGATCATTATGAAAACCAAATTTATCCAGCAGCTAACTTACACAAAGTCGATGATGCCAGTGAGTATGAGAAGGGCGTAATTGAAAAGTGTAATAAAATTGGAGATGCTGGTTACAATGAAAACGCTGAAACCTCTAACTTATGTGGGGATGACAAAAGCAAAGGAGTTGCAGAGAATTTTTCCACAGATCCTGCAAGTAATTTGGAATCTGTTAGTTGTCTATCTGAACTCCAAGTTAATCCAGTAACCAATCTACATGAAAGTGGTGATGCTGCTGGTGATTGTGAGAAGGATAAAACCGAAAAGTGTGATGTAACTGGGTATGATGGTGATCATGAGAGAGCTGAAAAGTATGCATCATCTGGTGAAGAAATAAGTAAAAGATCTACAGAAAAAACCTTTGTGGAGGACTCTACCACTAATTTGGAATCCATCATTACCCTCTCCGACTCTCAAGTTCATCCAGCGGGAAAGTTGCCTGATGTTGATGGCTCTGCTGATCGTGATGAGGGTGAAATTGAGAAATTTGTTATATCTGGAAATGAAAGCATGGAAGGATCAGTTGAAGAAAATATTCTGGTGAAGTCAGAATTGACCCCCGAGTCTTCTGCCAATCTAACTGGTTCAAAAGCTATTGTTGAAGATGCAGCCAATGGTTCTAGGATGGAGCTGGTTGAAACCAGTGGCAGAGATTTAGATAGCGGTTCGAATTCTAGCAAAGTTGCAGGAAAAATTCTATCAGATGCTCAGCAAGATGTGTTAGAAAATGAGATTGCTTGCAATGACAAAATCCTGAAAGAATTTGATGCCGGTGTTGCTGCTGGGTCAGATCATGGTCAGAATGTTGAATCGTTCCAGAAGAGTTCGGAGGACCATGGGAAGAAAGAACCAGGGTTTTCTGCTTTAGATACCGAATCTTCTGTTCAAAGTTTCATTGCCTCTGAAGATAGCAGCTCCCAAGAGGTCCATGCGGTTGCTTCTGGGATTACTTCTCAATCTTTGTTAGAGGAAGGCGATAATAACCTTGTCAGACAACAGCCAAGCTCAACTGCAATTGATGTGGACTCAAATAGCCCAACTGACAGTCTGGAAGCCAACTGGGGATCTTTCTCAGTTCTTTCCATGCAGTCTGATGCTGAAACTGTGCCACATACGCATTCCCAATCTTCATTAGGCGAAAACTCTGACTTGAAGAAGTCAAAAGTTGAATGTGAGAGTCAATACTCGGGCAAAACTGATATGTTTGAGGCACCATCTTTTATGACATTGGTTGAACCCCTAGGTGGGGATGATCAAAAAGCTACTGGTTCTGAAATCCTGACTGCAGTACAGAACCCAGAACAGCCAAAACCTGCATCTTTGCAAACTGGGTGGTTTCCTTCTATGACTCATGTTGTGAATGAGTCACCGGGGAGAAAGAAGAACGAAGAGATCATTGCCAAGGTGACAAACAGGAGCACTGGGAAGCAACACACTCCTTTGAAAAACCTTTTGGGGGAGGCCTCGAGTGAAAGCAACGGAAAGCCACTGAACCAAAAAGAAAGTCTTGCTCCTGCTCCTGCTCCTCAGAAAGATGATAAAGCTGCAATGGTCAGTGGTCCTGTAACAACAACAGTGAGTTCGATTCTGAATCCTGAATCGCCAACTGGTCAGGCTGCAAAGAAAGAGACTGCAAAAGAATGGAACTCGCCGGCCAGATATCCTTCAGACACCAAGAGTGAGAAGAGGAAGGTAAAGGGGAGATCCTGGGCACAATTTGTGTGCTGCGCTTCTGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1302

Amino Acids

139.3

Weight (kDa)

4.54

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AasI GACNNNNNNGTC 2 cut(s) 1675, 2728
Acc36I ACCTGC 1 cut(s) 3487
AccB1I GGYRCC 4 cut(s) 161, 416, 761, 3376
AccII CGCG 2 cut(s) 114, 116
AciI CCGC 9 cut(s) 69, 89, 132, 276, 348, 2079, 2594, 2829, 3098
AcoI YGGCCR 1 cut(s) 3819
AcsI RAATTY 6 cut(s) 2145, 2302, 2651, 2836, 2859, 2927
AcuI CTGAAG 9 cut(s) 609, 687, 834, 912, 1041, 1773, 1932, 3090, 3816
AcyI GRCGYC 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 3 cut(s) 1167, 2113, 3456
AfiI CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3410, 3411, 3412, 3622
AflIII ACRYGT 4 cut(s) 1039, 1162, 1168, 1195
AjnI CCWGG 3 cut(s) 579, 3016, 3872
AjuI GAANNNNNNNTTGG 2 cut(s) 3602, 3634
AleI CACNNNNGTG 1 cut(s) 96
AloI GAACNNNNNNTCC 2 cut(s) 616, 648
Alw21I GWGCWC 2 cut(s) 178, 3587
Alw26I GTCTC 7 cut(s) 193, 1387, 1481, 1677, 1836, 2052, 3788
Alw44I GTGCAC 1 cut(s) 174
AlwNI CAGNNNCTG 3 cut(s) 326, 1547, 2318
Ama87I CYCGRG 5 cut(s) 94, 388, 2726, 3352, 3631
AoxI GGCC 3 cut(s) 74, 3627, 3819
ApaLI GTGCAC 1 cut(s) 174
ApoI RAATTY 6 cut(s) 2145, 2302, 2651, 2836, 2859, 2927
ArsI GACNNNNNNTTYG 2 cut(s) 3655, 3687
Asp700I GAANNNNTTC 3 cut(s) 954, 1346, 2700
AspA2I CCTAGG 1 cut(s) 3406
AspLEI GCGC 4 cut(s) 116, 118, 164, 3896
AspS9I GGNCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
AsuC2I CCSGG 4 cut(s) 231, 389, 390, 3537
AsuII TTCGAA 1 cut(s) 2834
AsuNHI GCTAGC 1 cut(s) 1409
AvaI CYCGRG 5 cut(s) 94, 388, 2726, 3352, 3631
AvaII GGWCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
AvrII CCTAGG 1 cut(s) 3406
BaeGI GKGCMC 3 cut(s) 42, 178, 3880
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 4 cut(s) 161, 416, 761, 3376
BanII GRGCYC 2 cut(s) 28, 1537
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 3 cut(s) 477, 536, 2724
Bbv12I GWGCWC 2 cut(s) 178, 3587
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 2717, 2751
BciT130I CCWGG 3 cut(s) 581, 3018, 3874
BclI TGATCA 4 cut(s) 802, 2128, 2458, 3418
BcnI CCSGG 4 cut(s) 231, 389, 390, 3537
BcoDI GTCTC 7 cut(s) 193, 1387, 1481, 1677, 1836, 2052, 3788
BfaI CTAG 6 cut(s) 102, 842, 1410, 2787, 2841, 3407
BfmI CTRYAG 8 cut(s) 1704, 1731, 1863, 1890, 2062, 2106, 2514, 3450
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 3487
BglII AGATCT 2 cut(s) 13, 2510
BlnI CCTAGG 1 cut(s) 3406
BlpI GCTNAGC 1 cut(s) 2874
Bme1390I CCNGG 7 cut(s) 231, 389, 390, 581, 3018, 3537, 3874
Bme18I GGWCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
BmeT110I CYCGRG 5 cut(s) 94, 388, 2726, 3352, 3631
BmgT120I GGNCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
BmiI GGNNCC 8 cut(s) 136, 163, 219, 418, 763, 1534, 2077, 3378
BmrFI CCNGG 7 cut(s) 231, 389, 390, 581, 3018, 3537, 3874
BmrI ACTGGG 4 cut(s) 2454, 3235, 3504, 3597
BmtI GCTAGC 1 cut(s) 1413
BmuI ACTGGG 4 cut(s) 2454, 3235, 3504, 3597
BoxI GACNNNNGTC 1 cut(s) 1921
BpiI GAAGAC 3 cut(s) 477, 536, 2724
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 881, 913
BpmI CTGGAG 4 cut(s) 1692, 1701, 1860, 2076
Bpu1102I GCTNAGC 1 cut(s) 2874
Bpu14I TTCGAA 1 cut(s) 2834
BpuEI CTTGAG 1 cut(s) 2565
BpuMI CCSGG 4 cut(s) 231, 389, 390, 3537
BsaAI YACGTR 1 cut(s) 401
BsaBI GATNNNNATC 2 cut(s) 216, 2457
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 3 cut(s) 1481, 1677, 1836
BsaWI WCCGGW 3 cut(s) 365, 1420, 1787
BsaXI ACNNNNNCTCC 2 cut(s) 1362, 1392
Bsc4I CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3410, 3411, 3412, 3622
Bse118I RCCGGY 2 cut(s) 2936, 3817
Bse3DI GCAATG 5 cut(s) 703, 2914, 3060, 3561, 3720
Bse8I GATNNNNATC 2 cut(s) 216, 2457
BseBI CCWGG 3 cut(s) 581, 3018, 3874
BseGI GGATG 6 cut(s) 388, 814, 2281, 2587, 2796, 3421
BseJI GATNNNNATC 2 cut(s) 216, 2457
BseLI CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3410, 3411, 3412, 3622
BseMI GCAATG 5 cut(s) 703, 2914, 3060, 3561, 3720
BsePI GCGCGC 1 cut(s) 114
BseRI GAGGAG 1 cut(s) 3681
BseSI GKGCMC 3 cut(s) 42, 178, 3880
BseYI CCCAGC 1 cut(s) 2948
Bsh1236I CGCG 2 cut(s) 114, 116
BshFI GGCC 3 cut(s) 76, 3629, 3821
BshNI GGYRCC 4 cut(s) 161, 416, 761, 3376
BsiHKAI GWGCWC 2 cut(s) 178, 3587
BsiHKCI CYCGRG 5 cut(s) 94, 388, 2726, 3352, 3631
BsiSI CCGG 8 cut(s) 231, 366, 389, 1421, 1788, 2937, 3536, 3818
BslFI GGGAC 3 cut(s) 652, 998, 1681
BslI CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3410, 3411, 3412, 3622
BsmAI GTCTC 7 cut(s) 193, 1387, 1481, 1677, 1836, 2052, 3788
BsmFI GGGAC 3 cut(s) 652, 998, 1681
BsmI GAATGC 3 cut(s) 439, 1156, 3283
BsnI GGCC 3 cut(s) 76, 3629, 3821
Bso31I GGTCTC 3 cut(s) 1481, 1677, 1836
BsoBI CYCGRG 5 cut(s) 94, 388, 2726, 3352, 3631
Bsp119I TTCGAA 1 cut(s) 2834
Bsp1286I GDGCHC 6 cut(s) 28, 42, 178, 1537, 3587, 3880
Bsp1407I TGTACA 1 cut(s) 1165
Bsp1720I GCTNAGC 1 cut(s) 2874
Bsp19I CCATGG 1 cut(s) 3001
BspACI CCGC 9 cut(s) 69, 89, 132, 276, 348, 2079, 2594, 2829, 3098
BspANI GGCC 3 cut(s) 76, 3629, 3821
BspFNI CGCG 2 cut(s) 114, 116
BspHI TCATGA 1 cut(s) 2461
BspLI GGNNCC 8 cut(s) 136, 163, 219, 418, 763, 1534, 2077, 3378
BspMAI CTGCAG 3 cut(s) 1708, 1867, 3454
BspMI ACCTGC 1 cut(s) 3487
BspOI GCTAGC 1 cut(s) 1413
BspT104I TTCGAA 1 cut(s) 2834
BspT107I GGYRCC 4 cut(s) 161, 416, 761, 3376
BspTNI GGTCTC 3 cut(s) 1481, 1677, 1836
BsrDI GCAATG 5 cut(s) 703, 2914, 3060, 3561, 3720
BsrFI RCCGGY 2 cut(s) 2936, 3817
BsrGI TGTACA 1 cut(s) 1165
BssAI RCCGGY 2 cut(s) 2936, 3817
BssHII GCGCGC 1 cut(s) 114
BssNI GRCGYC 1 cut(s) 162
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 3 cut(s) 3001, 3406, 3567
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 3 cut(s) 581, 3018, 3874
Bst6I CTCTTC 5 cut(s) 1140, 1390, 2981, 3549, 3845
BstACI GRCGYC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 1165
BstBAI YACGTR 1 cut(s) 401
BstBI TTCGAA 1 cut(s) 2834
BstC8I GCNNGC 7 cut(s) 24, 116, 160, 1411, 1439, 2905, 3819
BstDSI CCRYGG 1 cut(s) 3001
BstF5I GGATG 6 cut(s) 388, 814, 2281, 2587, 2796, 3421
BstFNI CGCG 2 cut(s) 114, 116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 116, 118, 164, 3896
BstMAI GTCTC 7 cut(s) 193, 1387, 1481, 1677, 1836, 2052, 3788
BstMWI GCNNNNNNNGC 6 cut(s) 743, 1180, 1598, 1973, 2942, 3280
BstNI CCWGG 3 cut(s) 581, 3018, 3874
BstNSI RCATGY 4 cut(s) 1043, 1166, 1172, 1199
BstPAI GACNNNNGTC 1 cut(s) 1921
BstSCI CCNGG 7 cut(s) 229, 387, 388, 579, 3016, 3535, 3872
BstSFI CTRYAG 8 cut(s) 1704, 1731, 1863, 1890, 2062, 2106, 2514, 3450
BstSLI GKGCMC 3 cut(s) 42, 178, 3880
BstUI CGCG 2 cut(s) 114, 116
BstV2I GAAGAC 3 cut(s) 477, 536, 2724
BstX2I RGATCY 8 cut(s) 13, 217, 290, 1423, 2314, 2510, 3230, 3869
BstXI CCANNNNNNTGG 4 cut(s) 455, 1628, 2003, 2551
BstYI RGATCY 8 cut(s) 13, 217, 290, 1423, 2314, 2510, 3230, 3869
BsuRI GGCC 3 cut(s) 76, 3629, 3821
BtgI CCRYGG 1 cut(s) 3001
BtsCI GGATG 6 cut(s) 388, 814, 2281, 2587, 2796, 3421
BtsI GCAGTG 1 cut(s) 1381
BtsIMutI CAGTG 9 cut(s) 765, 1381, 1600, 2191, 2815, 3585, 3653, 3727, 3747
BveI ACCTGC 1 cut(s) 3487
Cac8I GCNNGC 7 cut(s) 24, 116, 160, 1411, 1439, 2905, 3819
CaiI CAGNNNCTG 3 cut(s) 326, 1547, 2318
CciI TCATGA 1 cut(s) 2461
CfoI GCGC 4 cut(s) 116, 118, 164, 3896
Cfr10I RCCGGY 2 cut(s) 2936, 3817
Cfr13I GGNCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
Cfr9I CCCGGG 1 cut(s) 388
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 2 cut(s) 120, 752
Csp6I GTAC 3 cut(s) 1166, 2112, 3455
CspCI CAANNNNNGTGG 4 cut(s) 3168, 3203, 3479, 3514
CspI CGGWCCG 1 cut(s) 233
CviQI GTAC 3 cut(s) 1166, 2112, 3455
DinI GGCGCC 1 cut(s) 163
DraI TTTAAA 1 cut(s) 1507
DraIII CACNNNGTG 1 cut(s) 55
DrdI GACNNNNNNGTC 2 cut(s) 1675, 2728
DseDI GACNNNNNNGTC 2 cut(s) 1675, 2728
EaeI YGGCCR 1 cut(s) 3819
Eam1104I CTCTTC 5 cut(s) 1140, 1390, 2981, 3549, 3845
EarI CTCTTC 5 cut(s) 1140, 1390, 2981, 3549, 3845
EciI GGCGGA 1 cut(s) 291
Eco130I CCWWGG 3 cut(s) 3001, 3406, 3567
Eco147I AGGCCT 1 cut(s) 3629
Eco24I GRGCYC 2 cut(s) 28, 1537
Eco31I GGTCTC 3 cut(s) 1481, 1677, 1836
Eco32I GATATC 1 cut(s) 3827
Eco47I GGWCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
Eco57I CTGAAG 9 cut(s) 609, 687, 834, 912, 1041, 1773, 1932, 3090, 3816
Eco88I CYCGRG 5 cut(s) 94, 388, 2726, 3352, 3631
EcoO109I RGGNCCY 1 cut(s) 2075
EcoRI GAATTC 1 cut(s) 2836
EcoRII CCWGG 3 cut(s) 579, 3016, 3872
EcoRV GATATC 1 cut(s) 3827
EcoT14I CCWWGG 3 cut(s) 3001, 3406, 3567
EcoT22I ATGCAT 5 cut(s) 514, 739, 996, 1398, 2485
EcoT38I GRGCYC 2 cut(s) 28, 1537
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 3 cut(s) 3001, 3406, 3567
FaqI GGGAC 3 cut(s) 652, 998, 1681
FauI CCCGC 3 cut(s) 62, 2086, 2587
FbaI TGATCA 4 cut(s) 802, 2128, 2458, 3418
FokI GGATG 6 cut(s) 395, 821, 2288, 2574, 2803, 3428
FriOI GRGCYC 2 cut(s) 28, 1537
FspBI CTAG 6 cut(s) 102, 842, 1410, 2787, 2841, 3407
GlaI GCGC 4 cut(s) 115, 117, 163, 3895
GsaI CCCAGC 1 cut(s) 2952
GsuI CTGGAG 4 cut(s) 1692, 1701, 1860, 2076
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 76, 3629, 3821
HapII CCGG 8 cut(s) 231, 366, 389, 1421, 1788, 2937, 3536, 3818
HgaI GACGC 2 cut(s) 120, 752
HhaI GCGC 4 cut(s) 116, 118, 164, 3896
Hin1I GRCGYC 1 cut(s) 162
Hin6I GCGC 4 cut(s) 114, 116, 162, 3894
HinP1I GCGC 4 cut(s) 114, 116, 162, 3894
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HpaII CCGG 8 cut(s) 231, 366, 389, 1421, 1788, 2937, 3536, 3818
Hpy166II GTNNAC 6 cut(s) 176, 577, 704, 1582, 1957, 3190
Hpy8I GTNNAC 6 cut(s) 176, 577, 704, 1582, 1957, 3190
HpyCH4IV ACGT 1 cut(s) 400
HpyF10VI GCNNNNNNNGC 6 cut(s) 743, 1180, 1598, 1973, 2942, 3280
HpySE526I ACGT 1 cut(s) 400
Hsp92I GRCGYC 1 cut(s) 162
HspAI GCGC 4 cut(s) 114, 116, 162, 3894
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 3817
KroNI GCCGGC 1 cut(s) 3819
Ksp22I TGATCA 4 cut(s) 802, 2128, 2458, 3418
LmnI GCTCC 9 cut(s) 252, 970, 1532, 2794, 3086, 3582, 3682, 3688, 3694
MaeI CTAG 6 cut(s) 102, 842, 1410, 2787, 2841, 3407
MaeII ACGT 1 cut(s) 400
MfeI CAATTG 1 cut(s) 3180
MflI RGATCY 8 cut(s) 13, 217, 290, 1423, 2314, 2510, 3230, 3869
MhlI GDGCHC 6 cut(s) 28, 42, 178, 1537, 3587, 3880
Mly113I GGCGCC 1 cut(s) 162
MmeI TCCRAC 2 cut(s) 357, 2598
Mph1103I ATGCAT 5 cut(s) 514, 739, 996, 1398, 2485
MroNI GCCGGC 1 cut(s) 3817
MroXI GAANNNNTTC 3 cut(s) 954, 1346, 2700
MseI TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2364
MslI CAYNNNNRTG 9 cut(s) 96, 210, 453, 978, 1167, 1196, 1365, 1542, 2388
MspA1I CMGCKG 2 cut(s) 2126, 2594
MspI CCGG 8 cut(s) 231, 366, 389, 1421, 1788, 2937, 3536, 3818
MspR9I CCNGG 7 cut(s) 231, 389, 390, 581, 3018, 3537, 3874
MunI CAATTG 1 cut(s) 3180
Mva1269I GAATGC 3 cut(s) 439, 1156, 3283
MvaI CCWGG 3 cut(s) 581, 3018, 3874
MvnI CGCG 2 cut(s) 114, 116
MwoI GCNNNNNNNGC 6 cut(s) 743, 1180, 1598, 1973, 2942, 3280
NaeI GCCGGC 1 cut(s) 3819
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 4 cut(s) 231, 389, 390, 3537
NcoI CCATGG 1 cut(s) 3001
NgoMIV GCCGGC 1 cut(s) 3817
NheI GCTAGC 1 cut(s) 1409
NlaIV GGNNCC 8 cut(s) 136, 163, 219, 418, 763, 1534, 2077, 3378
NmeAIII GCCGAG 2 cut(s) 190, 1407
NsiI ATGCAT 5 cut(s) 514, 739, 996, 1398, 2485
NspI RCATGY 4 cut(s) 1043, 1166, 1172, 1199
NspV TTCGAA 1 cut(s) 2834
OliI CACNNNNGTG 1 cut(s) 96
PaeR7I CTCGAG 1 cut(s) 3631
PagI TCATGA 1 cut(s) 2461
PauI GCGCGC 1 cut(s) 114
PceI AGGCCT 1 cut(s) 3629
PciI ACATGT 4 cut(s) 1039, 1162, 1168, 1195
PctI GAATGC 3 cut(s) 439, 1156, 3283
PdiI GCCGGC 1 cut(s) 3819
PdmI GAANNNNTTC 3 cut(s) 954, 1346, 2700
PluTI GGCGCC 1 cut(s) 165
Ppu21I YACGTR 1 cut(s) 401
PpuMI RGGWCCY 1 cut(s) 2075
PscI ACATGT 4 cut(s) 1039, 1162, 1168, 1195
PshAI GACNNNNGTC 1 cut(s) 1921
PsiI TTATAA 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 2075
Psp6I CCWGG 3 cut(s) 579, 3016, 3872
PspFI CCCAGC 1 cut(s) 2948
PspGI CCWGG 3 cut(s) 579, 3016, 3872
PspN4I GGNNCC 8 cut(s) 136, 163, 219, 418, 763, 1534, 2077, 3378
PspPI GGNCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
PspPPI RGGWCCY 1 cut(s) 2075
PspXI VCTCGAGB 1 cut(s) 3631
PstI CTGCAG 3 cut(s) 1708, 1867, 3454
PstNI CAGNNNCTG 3 cut(s) 326, 1547, 2318
PsuI RGATCY 8 cut(s) 13, 217, 290, 1423, 2314, 2510, 3230, 3869
PteI GCGCGC 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 2126
RsaI GTAC 3 cut(s) 1167, 2113, 3456
RsaNI GTAC 3 cut(s) 1166, 2112, 3455
RseI CAYNNNNRTG 9 cut(s) 96, 210, 453, 978, 1167, 1196, 1365, 1542, 2388
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SaqAI TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2364
Sau96I GGNCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
ScrFI CCNGG 7 cut(s) 231, 389, 390, 581, 3018, 3537, 3874
SduI GDGCHC 6 cut(s) 28, 42, 178, 1537, 3587, 3880
SfcI CTRYAG 8 cut(s) 1704, 1731, 1863, 1890, 2062, 2106, 2514, 3450
SfoI GGCGCC 1 cut(s) 163
Sfr274I CTCGAG 1 cut(s) 3631
SfuI TTCGAA 1 cut(s) 2834
SinI GGWCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
SlaI CTCGAG 1 cut(s) 3631
SmaI CCCGGG 1 cut(s) 390
SmiMI CAYNNNNRTG 9 cut(s) 96, 210, 453, 978, 1167, 1196, 1365, 1542, 2388
SmlI CTYRAG 2 cut(s) 2580, 3631
SmoI CTYRAG 2 cut(s) 2580, 3631
SseBI AGGCCT 1 cut(s) 3629
SsiI CCGC 9 cut(s) 69, 89, 132, 276, 348, 2079, 2594, 2829, 3098
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 1 cut(s) 2701
SspMI CTAG 6 cut(s) 102, 842, 1410, 2787, 2841, 3407
StuI AGGCCT 1 cut(s) 3629
StyD4I CCNGG 7 cut(s) 229, 387, 388, 579, 3016, 3535, 3872
StyI CCWWGG 3 cut(s) 3001, 3406, 3567
TaiI ACGT 1 cut(s) 403
TaqI TCGA 5 cut(s) 2013, 2175, 2834, 3632, 3749
TaqII GACCGA 2 cut(s) 548, 1505
TatI WGTACW 2 cut(s) 1165, 3454
Tru1I TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2364
Tru9I TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2364
TscAI CASTG 9 cut(s) 772, 1381, 1600, 2191, 2815, 3592, 3660, 3727, 3747
TspGWI ACGGA 2 cut(s) 432, 3660
TspMI CCCGGG 1 cut(s) 388
TspRI CASTG 9 cut(s) 772, 1381, 1600, 2191, 2815, 3592, 3660, 3727, 3747
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 8 cut(s) 233, 363, 1631, 2006, 2075, 2998, 3091, 3725
XapI RAATTY 6 cut(s) 2145, 2302, 2651, 2836, 2859, 2927
XceI RCATGY 4 cut(s) 1043, 1166, 1172, 1199
XhoI CTCGAG 1 cut(s) 3631
XmaI CCCGGG 1 cut(s) 388
XmaJI CCTAGG 1 cut(s) 3406
XmnI GAANNNNTTC 3 cut(s) 954, 1346, 2700
XspI CTAG 6 cut(s) 102, 842, 1410, 2787, 2841, 3407
Zsp2I ATGCAT 5 cut(s) 514, 739, 996, 1398, 2485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.