MD04G1021500.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001352
Physical Location & Seq
Forward (+)
2545619 .. 2551818
6200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1021500.v1.1.491

Sequence Viewer

Length: 3060 bp
ATGGATGCTCAAGATCTAGTGAGAAGTCACAGCACAGGGCATGAGAGCCATGGAGGAGTCCATGTTTGCAGCAAATGTGGGTGGCCCTTTCCGAACCCACACCCAAGTGCCAGGCACAGGCGTGCTCATAAGAGGATTTGTGGAACCATTGATGGCTACAAGTTGGTTGGCTTACAGGAGAACACCCAGTCAGATGATGAGCACTCCGATGACGACCGCAAAACCCCAAGTTCGAAAGTATTGGAAAGAAGTAACAACGAAAAGGGCAGTGGTCTAATTGGGGAAGGATCTGTCAGATCAGAAGATGCGGTGTTCTCAGATGCTGTTGCAGAATTTTCGGATAGTGGATCTGGTGCAGGAACTGGAGAAGGGTTAGAAGATGTTTTGAAATCAGCAACTAGTGTGGAAAAGGTTGCCAAGAATGATTTGAATGCCATCCAATCCCTTAATGATGGGGAAATCAACGAGAATACAACTAATCAGCTGGGCTATGCACCAGAGTCCCAAGGATCTATGTCAATCACTGGCATCGATTCAATGAAGAGGCCAGAGTCAGCTGTGCAGGATGATAGGAATGGTTCAGAAGATGACGTACACCCTGTTAACGATGTATCAGAAGATAGCAAGAAGGCCCATGCTACTGACAGTGTGATTGAGAGCTCTTTAACATCCATTTCACAGGAGGGCCAGGTGAAGGAAATCAAAGCAAGTGATCAAGATAGAAACTTACCATACGGTTTGGTCGCACCCAATGAAGTTTCCAGTGAAACAGCTGAGGGTGCCTCTAAGTTAGAAAGAACAGTTGAAGTGACTTCAGACTCTTTGCGAGATATTGAAGTTGTTCCATCAGAGAAAGAGCATATTCATGTGTTTGATTTGGATATTCCCCAAGCTGGTCTTTCCCCTGATGTTGAATCTGTTGAACATGCACAAGCTTCTGTTAATACCACAGAAAATAAGGTGGATACCACAGACGAGATGCACTGTTTCAGTTCTGGTGGATTAACTGAGAGTAGCAACTCGAAGGGAGAAGGGAGTGCAAATGTACATGTGCTTTCTGTGCCTGATGACCTACCGGTGGTAGACAATGCTGCGGCAATGCTTGAAGGTTTCAAAACCTACAAAGAAGAGAAACTAGACCAGCCCACTTTTTTGGACTCTTGGGAACAATTTAATGACGAGGAAAACGGTGTTAAAAATCCTGTTTCAAATGACAATTCTCAGATTTTCCAGTCAAGTAATTTAAGTGGAGGCACTGTGGCCTCTTCAGATATGCATGATTTAGAAGCTAGCCATGAACTGGATGGAGGCAGCAGCAAGCCAATGGTTAAAGAAGTGCCTTTTGAAGAAGCCAATGTGTCTCAGGTTGAGATCAAGACTACTGAAAACCAAGGTTCAGATGAAAAATCACCCCCTGCTGACATTGTGATGCCTCAGATAGCTAAAAGCTTGGTTGGCTCTCCTGAAGGACAGGAGACTTATGATAATTATGAGTCGTTACAGGTGAACTTTCCAGAAAGTGATACAAATGTCGCATCTGATGCAAATTCCTTGAGCAGTACTGGTATAAATCAAACAACACACTCTGTTGATCTGGATGATAACGAGAAAGATGAAAAATGTGATTTGAGTGCAGATCATAGCAGCAAAGGACCTGTAGAAGGAAATGTTATTGACGATGGTGATCATGAGACTGAAAAGTATGCTATAACTGAGGATGAGTACATTGAAGTATCTATGGAGAAAATTGTTGCTGAGAACCCTATTACTAGTTTGGAAAATACCAGTTGTCTCTCCAAACTTCAAGTTAATTCAACAGATATACTGAATGGCGATGACTCTGGTGATCACGATAAGGGTGAAACTGAAAAAAGTGTTGTATCTGGAAATAAAAGCAGGGGAGGATCTATTGAGGAGAATCTTTTGGTGAAGCCAAAACTGAACCTGGACAGCGGTTCCAATTCTAGCAGAGCTGACCTCAATGTAGTTTCAGATACAAAGAAAAGTGTGATGGAAAGTGAGATTGATTGCAATGAAAAGGTCCAGCTAGAAGTTGGGGGCAGTACTGCTGCTGGGTCAGATCATGGTCCCAATGTTGAATCGTCACAGAAGAGTTTAATGGACTGTGGAATGAAAGAAGTAGGTCTGTCCTCTTTAGATACTGAATCTTCTGTTCCAAATCTGTCCTCTTTAGCTACTGAATCTTCTGTTCCAAGTTTCACCGCTGCTGAAGATAACTGCTCCAGAGAGCTCCTTGGGGTTTCTTTGAAGGAAGAGAGTGATAAACTTGTTGAGCAACAGACAAGTTCGTCTGCACTTGATGTTGTTGACTCGAATAGCCAAACTGACAGCCTCGAAGGCAACTGGGGATCTGTTTCAGTTTATTCTATCCAGTCGGAGGCACAAGCTGTTATTGATGCTGAAGCTGTACCACCTACAGATCCCCAAGCTTCATTGGAAGGGAAGGTGAAACTTGCATCTGAGAGGCAATACTCTGACAAATCTGATACGTTTGAGGCACCGTCTTTCATGACATTGGTTGAACCTGGAGGCGTTGATAATCAAAAGGCTACTGCTGCTGAAATCAAGACAGCACAGAACCCGGAACAGCCAAGACCTGCACCTTTGCAGGCTGGGTGGTTTCCTTCTATTACTCATGTTGTGAATGAATCACCGGGGAGAAAGAAGAACGAAGAGATCATCGCCAAGGTTACAAACTGGAGCACTGGGAAGCAACACACTCCTTTGAAAAACCTTTTGGGTGAGGCCTACCTAGAGAACAAAGCCAAGTCACCGAACCAAAAAGAAAGTCAAGCCCCTGCGCTTCAGAAAGATGATAAAGCAGTGACGGGGAAGGACAATGGTCGTGTAACGACGACAGTGAACTCAATTCTGGCTCCCGAATCACCGACAGGTCAGGCATCTAGGAAAGACAATGCAAAAGAATGGAACTCTCCGGCAAGGTACCCTACAGAGATCAAGAGTGAAAAGCGGAAAGCCAAGGGGAGACCGTACTGGGCACAATTTTTATGCTGCTCATCTGCGCACCAGCATCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1020

Amino Acids

109.35

Weight (kDa)

4.54

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2308
Acc16I TGCGCA 1 cut(s) 3045
Acc36I ACCTGC 1 cut(s) 2626
Acc65I GGTACC 1 cut(s) 2964
AccB1I GGYRCC 3 cut(s) 779, 2518, 2964
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccI GTMKAC 1 cut(s) 1083
AciI CCGC 6 cut(s) 217, 308, 1094, 1953, 2223, 2992
AclWI GGATC 6 cut(s) 295, 355, 517, 1912, 2377, 2435
AcsI RAATTY 2 cut(s) 332, 1546
AcuI CTGAAG 6 cut(s) 798, 1251, 1485, 2250, 2442, 2810
AfaI GTAC 8 cut(s) 594, 1047, 1561, 1724, 2065, 2430, 2966, 3014
AfiI CCNNNNNNNGG 7 cut(s) 117, 694, 893, 1078, 1300, 1661, 2398
AflIII ACRYGT 1 cut(s) 1048
AgeI ACCGGT 1 cut(s) 1075
AhlI ACTAGT 2 cut(s) 398, 1769
AjnI CCWGG 4 cut(s) 110, 687, 1944, 2545
AjuI GAANNNNNNNTTGG 2 cut(s) 2741, 2773
AleI CACNNNNGTG 2 cut(s) 105, 120
Alw21I GWGCWC 5 cut(s) 127, 204, 662, 2253, 2726
Alw26I GTCTC 5 cut(s) 1365, 1469, 1685, 1796, 3001
AlwI GGATC 6 cut(s) 295, 355, 517, 1912, 2377, 2435
AlwNI CAGNNNCTG 2 cut(s) 323, 362
AoxI GGCC 6 cut(s) 83, 545, 630, 685, 1260, 2766
ApeKI GCWGC 9 cut(s) 69, 1091, 1311, 1314, 1644, 2069, 2225, 2576, 3033
ApoI RAATTY 2 cut(s) 332, 1546
ArsI GACNNNNNNTTYG 2 cut(s) 2794, 2826
AsiGI ACCGGT 1 cut(s) 1075
Asp700I GAANNNNTTC 1 cut(s) 840
Asp718I GGTACC 1 cut(s) 2964
AspLEI GCGC 2 cut(s) 2824, 3046
AspS9I GGNCC 6 cut(s) 84, 631, 685, 1652, 2041, 2087
AsuC2I CCSGG 2 cut(s) 2603, 2676
AsuII TTCGAA 1 cut(s) 233
AsuNHI GCTAGC 1 cut(s) 1289
AvaII GGWCC 3 cut(s) 1652, 2041, 2087
BaeGI GKGCMC 1 cut(s) 3022
BaeI ACNNNNGTAYC 2 cut(s) 1515, 1548
BanI GGYRCC 3 cut(s) 779, 2518, 2964
BanII GRGCYC 2 cut(s) 662, 2253
BarI GAAGNNNNNNTAC 2 cut(s) 576, 608
Bbv12I GWGCWC 5 cut(s) 127, 204, 662, 2253, 2726
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 9 cut(s) 81, 1078, 1323, 1326, 1656, 2056, 2212, 2563, 3020
BccI CCATC 7 cut(s) 146, 443, 446, 853, 1298, 1673, 2005
BcgI CGANNNNNNTGC 2 cut(s) 318, 352
BciT130I CCWGG 4 cut(s) 112, 689, 1946, 2547
BciVI GTATCC 1 cut(s) 958
BclI TGATCA 3 cut(s) 712, 1684, 1846
BcnI CCSGG 2 cut(s) 2603, 2676
BcoDI GTCTC 5 cut(s) 1365, 1469, 1685, 1796, 3001
BcuI ACTAGT 2 cut(s) 398, 1769
BfaI CTAG 9 cut(s) 17, 399, 1136, 1290, 1770, 1965, 2048, 2774, 2925
BfmI CTRYAG 3 cut(s) 1656, 2436, 2970
BfuAI ACCTGC 1 cut(s) 2626
BfuI GTATCC 1 cut(s) 958
BglI GCCNNNNNGGC 1 cut(s) 2358
BglII AGATCT 1 cut(s) 13
BmcAI AGTACT 2 cut(s) 1561, 2065
Bme1390I CCNGG 6 cut(s) 112, 689, 1946, 2547, 2603, 2676
Bme18I GGWCC 3 cut(s) 1652, 2041, 2087
BmgT120I GGNCC 6 cut(s) 84, 631, 685, 1652, 2041, 2087
BmiI GGNNCC 7 cut(s) 145, 781, 1957, 2089, 2520, 2898, 2966
BmrFI CCNGG 6 cut(s) 112, 689, 1946, 2547, 2603, 2676
BmrI ACTGGG 4 cut(s) 181, 2374, 2736, 3025
BmtI GCTAGC 1 cut(s) 1293
BmuI ACTGGG 4 cut(s) 181, 2374, 2736, 3025
BoxI GACNNNNGTC 1 cut(s) 2862
BplI GAGNNNNNCTC 4 cut(s) 767, 799, 1962, 1994
BpmI CTGGAG 4 cut(s) 384, 2227, 2568, 2740
Bpu10I CCTNAGC 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 1573
BpuMI CCSGG 2 cut(s) 2603, 2676
Bsa29I ATCGAT 1 cut(s) 531
BsaBI GATNNNNATC 1 cut(s) 1683
BsaI GGTCTC 1 cut(s) 3001
BsaJI CCNNGG 7 cut(s) 49, 505, 1390, 2254, 2675, 2706, 3000
BsaWI WCCGGW 1 cut(s) 1075
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 1242, 1272
Bsc4I CCNNNNNNNGG 7 cut(s) 117, 694, 893, 1078, 1300, 1661, 2398
Bse118I RCCGGY 1 cut(s) 1075
Bse3DI GCAATG 2 cut(s) 1104, 2038
Bse8I GATNNNNATC 1 cut(s) 1683
BseBI CCWGG 4 cut(s) 112, 689, 1946, 2547
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 7 cut(s) 49, 505, 1390, 2254, 2675, 2706, 3000
BseGI GGATG 8 cut(s) 10, 435, 571, 668, 1309, 1603, 1723, 3052
BseJI GATNNNNATC 1 cut(s) 1683
BseLI CCNNNNNNNGG 7 cut(s) 117, 694, 893, 1078, 1300, 1661, 2398
BseMI GCAATG 2 cut(s) 1104, 2038
BseMII CTCAG 9 cut(s) 330, 765, 999, 1235, 1376, 1448, 1704, 1746, 2472
BseRI GAGGAG 2 cut(s) 69, 1928
BseSI GKGCMC 1 cut(s) 3022
BseXI GCAGC 9 cut(s) 81, 1078, 1323, 1326, 1656, 2056, 2212, 2563, 3020
BseYI CCCAGC 3 cut(s) 484, 2072, 2633
BsgI GTGCAG 5 cut(s) 375, 581, 1653, 2298, 2604
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 6 cut(s) 85, 547, 632, 687, 1262, 2768
BshNI GGYRCC 3 cut(s) 779, 2518, 2964
BshTI ACCGGT 1 cut(s) 1075
BshVI ATCGAT 1 cut(s) 531
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 5 cut(s) 127, 204, 662, 2253, 2726
BsiSI CCGG 4 cut(s) 1076, 2603, 2675, 2957
BslFI GGGAC 2 cut(s) 487, 2073
BslI CCNNNNNNNGG 7 cut(s) 117, 694, 893, 1078, 1300, 1661, 2398
BsmAI GTCTC 5 cut(s) 1365, 1469, 1685, 1796, 3001
BsmFI GGGAC 2 cut(s) 487, 2073
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 6 cut(s) 85, 547, 632, 687, 1262, 2768
Bso31I GGTCTC 1 cut(s) 3001
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 6 cut(s) 127, 204, 662, 2253, 2726, 3022
Bsp1407I TGTACA 1 cut(s) 1045
Bsp19I CCATGG 1 cut(s) 49
BspACI CCGC 6 cut(s) 217, 308, 1094, 1953, 2223, 2992
BspANI GGCC 6 cut(s) 85, 547, 632, 687, 1262, 2768
BspCNI CTCAG 9 cut(s) 329, 766, 1000, 1234, 1375, 1447, 1705, 1747, 2473
BspDI ATCGAT 1 cut(s) 531
BspHI TCATGA 2 cut(s) 1687, 2529
BspLI GGNNCC 7 cut(s) 145, 781, 1957, 2089, 2520, 2898, 2966
BspMI ACCTGC 1 cut(s) 2626
BspOI GCTAGC 1 cut(s) 1293
BspPI GGATC 6 cut(s) 295, 355, 517, 1912, 2377, 2435
BspT104I TTCGAA 1 cut(s) 233
BspT107I GGYRCC 3 cut(s) 779, 2518, 2964
BspTNI GGTCTC 1 cut(s) 3001
BsrDI GCAATG 2 cut(s) 1104, 2038
BsrFI RCCGGY 1 cut(s) 1075
BsrGI TGTACA 1 cut(s) 1045
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 7 cut(s) 49, 505, 1390, 2254, 2675, 2706, 3000
BssT1I CCWWGG 6 cut(s) 49, 505, 1390, 2254, 2706, 3000
Bst2UI CCWGG 4 cut(s) 112, 689, 1946, 2547
Bst6I CTCTTC 6 cut(s) 536, 1122, 1270, 2105, 2268, 2688
BstAPI GCANNNNNTGC 1 cut(s) 1541
BstAUI TGTACA 1 cut(s) 1045
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 4 cut(s) 123, 1291, 1319, 2631
BstDSI CCRYGG 1 cut(s) 49
BstENI CCTNNNNNAGG 1 cut(s) 1659
BstF5I GGATG 8 cut(s) 10, 435, 571, 668, 1309, 1603, 1723, 3052
BstHHI GCGC 2 cut(s) 2824, 3046
BstMAI GTCTC 5 cut(s) 1365, 1469, 1685, 1796, 3001
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 6 cut(s) 779, 1060, 1455, 1541, 2358, 2576
BstNI CCWGG 4 cut(s) 112, 689, 1946, 2547
BstNSI RCATGY 2 cut(s) 929, 1052
BstPAI GACNNNNGTC 1 cut(s) 2862
BstSCI CCNGG 6 cut(s) 110, 687, 1944, 2545, 2601, 2674
BstSFI CTRYAG 3 cut(s) 1656, 2436, 2970
BstSLI GKGCMC 1 cut(s) 3022
BstV1I GCAGC 9 cut(s) 81, 1078, 1323, 1326, 1656, 2056, 2212, 2563, 3020
BstX2I RGATCY 7 cut(s) 13, 287, 347, 509, 1904, 2369, 2440
BstXI CCANNNNNNTGG 1 cut(s) 1153
BstYI RGATCY 7 cut(s) 13, 287, 347, 509, 1904, 2369, 2440
Bsu15I ATCGAT 1 cut(s) 531
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 6 cut(s) 85, 547, 632, 687, 1262, 2768
BsuTUI ATCGAT 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 49
BtgZI GCGATG 2 cut(s) 1848, 2686
BtsCI GGATG 8 cut(s) 10, 435, 571, 668, 1309, 1603, 1723, 3052
BtsI GCAGTG 2 cut(s) 274, 2850
BtsIMutI CAGTG 9 cut(s) 274, 522, 652, 769, 982, 1254, 2724, 2850, 2886
BveI ACCTGC 1 cut(s) 2626
Cac8I GCNNGC 4 cut(s) 123, 1291, 1319, 2631
CaiI CAGNNNCTG 2 cut(s) 323, 362
CciI TCATGA 2 cut(s) 1687, 2529
CfoI GCGC 2 cut(s) 2824, 3046
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 6 cut(s) 84, 631, 685, 1652, 2041, 2087
ClaI ATCGAT 1 cut(s) 531
Csp6I GTAC 8 cut(s) 593, 1046, 1560, 1723, 2064, 2429, 2965, 3013
CspAI ACCGGT 1 cut(s) 1075
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviQI GTAC 8 cut(s) 593, 1046, 1560, 1723, 2064, 2429, 2965, 3013
DrdI GACNNNNNNGTC 1 cut(s) 2308
DseDI GACNNNNNNGTC 1 cut(s) 2308
Eam1104I CTCTTC 6 cut(s) 536, 1122, 1270, 2105, 2268, 2688
EarI CTCTTC 6 cut(s) 536, 1122, 1270, 2105, 2268, 2688
Ecl136II GAGCTC 2 cut(s) 660, 2251
Eco130I CCWWGG 6 cut(s) 49, 505, 1390, 2254, 2706, 3000
Eco147I AGGCCT 1 cut(s) 2768
Eco24I GRGCYC 2 cut(s) 662, 2253
Eco31I GGTCTC 1 cut(s) 3001
Eco47I GGWCC 3 cut(s) 1652, 2041, 2087
Eco53kI GAGCTC 2 cut(s) 660, 2251
Eco57I CTGAAG 6 cut(s) 798, 1251, 1485, 2250, 2442, 2810
EcoICRI GAGCTC 2 cut(s) 660, 2251
EcoNI CCTNNNNNAGG 1 cut(s) 1659
EcoO109I RGGNCCY 1 cut(s) 1652
EcoRII CCWGG 4 cut(s) 110, 687, 1944, 2545
EcoT14I CCWWGG 6 cut(s) 49, 505, 1390, 2254, 2706, 3000
EcoT22I ATGCAT 1 cut(s) 1278
EcoT38I GRGCYC 2 cut(s) 662, 2253
ErhI CCWWGG 6 cut(s) 49, 505, 1390, 2254, 2706, 3000
FalI AAGNNNNNCTT 2 cut(s) 882, 914
FaqI GGGAC 2 cut(s) 487, 2073
FbaI TGATCA 3 cut(s) 712, 1684, 1846
FblI GTMKAC 1 cut(s) 1083
FokI GGATG 8 cut(s) 17, 422, 578, 655, 1316, 1610, 1730, 3039
FriOI GRGCYC 2 cut(s) 662, 2253
FspBI CTAG 9 cut(s) 17, 399, 1136, 1290, 1770, 1965, 2048, 2774, 2925
FspI TGCGCA 1 cut(s) 3045
GlaI GCGC 2 cut(s) 2823, 3045
GsaI CCCAGC 3 cut(s) 488, 2076, 2637
GsuI CTGGAG 4 cut(s) 384, 2227, 2568, 2740
HaeIII GGCC 6 cut(s) 85, 547, 632, 687, 1262, 2768
HapII CCGG 4 cut(s) 1076, 2603, 2675, 2957
HhaI GCGC 2 cut(s) 2824, 3046
Hin6I GCGC 2 cut(s) 2822, 3044
HinP1I GCGC 2 cut(s) 2822, 3044
HincII GTYRAC 2 cut(s) 604, 2329
HindII GTYRAC 2 cut(s) 604, 2329
HindIII AAGCTT 3 cut(s) 933, 1447, 2448
HpaI GTTAAC 1 cut(s) 604
HpaII CCGG 4 cut(s) 1076, 2603, 2675, 2957
Hpy166II GTNNAC 6 cut(s) 595, 604, 1084, 1507, 2329, 2884
Hpy8I GTNNAC 6 cut(s) 595, 604, 1084, 1507, 2329, 2884
Hpy99I CGWCG 1 cut(s) 2878
HpyCH4IV ACGT 2 cut(s) 591, 2510
HpyF10VI GCNNNNNNNGC 6 cut(s) 779, 1060, 1455, 1541, 2358, 2576
HpySE526I ACGT 2 cut(s) 591, 2510
HspAI GCGC 2 cut(s) 2822, 3044
KpnI GGTACC 1 cut(s) 2968
Ksp22I TGATCA 3 cut(s) 712, 1684, 1846
KspAI GTTAAC 1 cut(s) 604
LmnI GCTCC 4 cut(s) 2246, 2256, 2721, 2902
Lsp1109I GCAGC 9 cut(s) 81, 1078, 1323, 1326, 1656, 2056, 2212, 2563, 3020
MaeI CTAG 9 cut(s) 17, 399, 1136, 1290, 1770, 1965, 2048, 2774, 2925
MaeII ACGT 2 cut(s) 591, 2510
MaeIII GTNAC 9 cut(s) 26, 251, 808, 1497, 2103, 2710, 2790, 2845, 2869
MflI RGATCY 7 cut(s) 13, 287, 347, 509, 1904, 2369, 2440
MhlI GDGCHC 6 cut(s) 127, 204, 662, 2253, 2726, 3022
MlyI GAGTC 8 cut(s) 66, 509, 560, 812, 1151, 1502, 1832, 2324
MmeI TCCRAC 1 cut(s) 2376
Mph1103I ATGCAT 1 cut(s) 1278
MroXI GAANNNNTTC 1 cut(s) 840
MslI CAYNNNNRTG 5 cut(s) 105, 120, 207, 864, 1427
MspA1I CMGCKG 5 cut(s) 484, 557, 773, 1953, 2225
MspI CCGG 4 cut(s) 1076, 2603, 2675, 2957
MspR9I CCNGG 6 cut(s) 112, 689, 1946, 2547, 2603, 2676
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 4 cut(s) 112, 689, 1946, 2547
MwoI GCNNNNNNNGC 6 cut(s) 779, 1060, 1455, 1541, 2358, 2576
NciI CCSGG 2 cut(s) 2603, 2676
NcoI CCATGG 1 cut(s) 49
NheI GCTAGC 1 cut(s) 1289
NlaIV GGNNCC 7 cut(s) 145, 781, 1957, 2089, 2520, 2898, 2966
NmuCI GTSAC 5 cut(s) 26, 808, 2103, 2790, 2845
NsbI TGCGCA 1 cut(s) 3045
NsiI ATGCAT 1 cut(s) 1278
NspI RCATGY 2 cut(s) 929, 1052
NspV TTCGAA 1 cut(s) 233
OliI CACNNNNGTG 2 cut(s) 105, 120
PagI TCATGA 2 cut(s) 1687, 2529
PceI AGGCCT 1 cut(s) 2768
PciI ACATGT 1 cut(s) 1048
PctI GAATGC 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 840
PfeI GAWTC 8 cut(s) 533, 914, 1918, 2099, 2165, 2201, 2669, 2903
PflMI CCANNNNNTGG 1 cut(s) 1300
PinAI ACCGGT 1 cut(s) 1075
PleI GAGTC 8 cut(s) 65, 508, 559, 812, 1151, 1501, 1832, 2324
PpsI GAGTC 8 cut(s) 65, 508, 559, 812, 1151, 1501, 1832, 2324
PpuMI RGGWCCY 1 cut(s) 1652
PscI ACATGT 1 cut(s) 1048
PshAI GACNNNNGTC 1 cut(s) 2862
Psp124BI GAGCTC 2 cut(s) 662, 2253
Psp5II RGGWCCY 1 cut(s) 1652
Psp6I CCWGG 4 cut(s) 110, 687, 1944, 2545
PspFI CCCAGC 3 cut(s) 484, 2072, 2633
PspGI CCWGG 4 cut(s) 110, 687, 1944, 2545
PspN4I GGNNCC 7 cut(s) 145, 781, 1957, 2089, 2520, 2898, 2966
PspPI GGNCC 6 cut(s) 84, 631, 685, 1652, 2041, 2087
PspPPI RGGWCCY 1 cut(s) 1652
PstNI CAGNNNCTG 2 cut(s) 323, 362
PsuI RGATCY 7 cut(s) 13, 287, 347, 509, 1904, 2369, 2440
PvuII CAGCTG 3 cut(s) 484, 557, 773
RsaI GTAC 8 cut(s) 594, 1047, 1561, 1724, 2065, 2430, 2966, 3014
RsaNI GTAC 8 cut(s) 593, 1046, 1560, 1723, 2064, 2429, 2965, 3013
RseI CAYNNNNRTG 5 cut(s) 105, 120, 207, 864, 1427
SacI GAGCTC 2 cut(s) 662, 2253
Sau96I GGNCC 6 cut(s) 84, 631, 685, 1652, 2041, 2087
ScaI AGTACT 2 cut(s) 1561, 2065
SchI GAGTC 8 cut(s) 66, 509, 560, 812, 1151, 1502, 1832, 2324
ScrFI CCNGG 6 cut(s) 112, 689, 1946, 2547, 2603, 2676
SduI GDGCHC 6 cut(s) 127, 204, 662, 2253, 2726, 3022
SfcI CTRYAG 3 cut(s) 1656, 2436, 2970
SfuI TTCGAA 1 cut(s) 233
SinI GGWCC 3 cut(s) 1652, 2041, 2087
SmiMI CAYNNNNRTG 5 cut(s) 105, 120, 207, 864, 1427
SmlI CTYRAG 2 cut(s) 9, 1552
SmoI CTYRAG 2 cut(s) 9, 1552
SpeI ACTAGT 2 cut(s) 398, 1769
SseBI AGGCCT 1 cut(s) 2768
SsiI CCGC 6 cut(s) 217, 308, 1094, 1953, 2223, 2992
SspMI CTAG 9 cut(s) 17, 399, 1136, 1290, 1770, 1965, 2048, 2774, 2925
SstI GAGCTC 2 cut(s) 662, 2253
StuI AGGCCT 1 cut(s) 2768
StyD4I CCNGG 6 cut(s) 110, 687, 1944, 2545, 2601, 2674
StyI CCWWGG 6 cut(s) 49, 505, 1390, 2254, 2706, 3000
TaiI ACGT 2 cut(s) 594, 2513
TaqI TCGA 5 cut(s) 233, 531, 1022, 2333, 2355
TatI WGTACW 4 cut(s) 1045, 1559, 1722, 2063
TauI GCSGC 1 cut(s) 1097
TfiI GAWTC 8 cut(s) 533, 914, 1918, 2099, 2165, 2201, 2669, 2903
TscAI CASTG 9 cut(s) 274, 529, 652, 769, 989, 1261, 2731, 2850, 2886
TseFI GTSAC 5 cut(s) 26, 808, 2103, 2790, 2845
TseI GCWGC 9 cut(s) 69, 1091, 1311, 1314, 1644, 2069, 2225, 2576, 3033
Tsp45I GTSAC 5 cut(s) 26, 808, 2103, 2790, 2845
TspGWI ACGGA 1 cut(s) 3045
TspRI CASTG 9 cut(s) 274, 529, 652, 769, 989, 1261, 2731, 2850, 2886
Van91I CCANNNNNTGG 1 cut(s) 1300
VpaK11BI GGWCC 3 cut(s) 1652, 2041, 2087
XagI CCTNNNNNAGG 1 cut(s) 1659
XapI RAATTY 2 cut(s) 332, 1546
XceI RCATGY 2 cut(s) 929, 1052
XcmI CCANNNNNNNNNTGG 2 cut(s) 1301, 2051
XmiI GTMKAC 1 cut(s) 1083
XmnI GAANNNNTTC 1 cut(s) 840
XspI CTAG 9 cut(s) 17, 399, 1136, 1290, 1770, 1965, 2048, 2774, 2925
ZrmI AGTACT 2 cut(s) 1561, 2065
Zsp2I ATGCAT 1 cut(s) 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.