Rh2AG281000

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001352
Physical Location & Seq
Reverse (-)
34156113 .. 34156784
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG281000.1

Sequence Viewer

Length: 672 bp
ATGCATGTATCAGTTCTTTCCCCGCAATCTGATGCTGAAGCTGTGCCACATATGCATTCCCAGTCTTCATTAGAAGAAAACTGTGACTTAAAGAAGTCAAAAGTTGAATGTGAGAATCAGTACTTGGACAAATCTGATATGTTTGAGGCACCATCTTTCATGAGATTGGTTGAACCCCGAGGTGGGGATGATCAAAAAGCTACTGGTTTTGAAATCCTGACTGCAGTACAGAGCCCAGAACAGCCAAAACCTGCATCTTCTCAAACTGGGTGGTTTCCCTCCATGACTCATGTTGTGAATGAGTCGCAGGGGAGAGAGAAGAATGAAGAGATCATTGCTAAGGTGACAAACGGGAGCACTGGGAAGCAACACACTCCTTTGAAAAGCCTTTTCGGGGAGGCCTCAAGTGAAAGCAAAGGAAAGCCACTGAACCAAAAAGAAAGTCTTGCTCCTGCTCCTGAGCAAAATGATAAAGCTGCAAAGGTCAGTGGTCCTGTAACGACAGTGAGCACGATTCTGAATCCTGAATCGCCGACTGGTCAGGCTGCTAAGAAAGAGATTGCAAAAAATGGAACTCGCCGGCCAGATATCCTTCAGACACTAAAGAGGGAGAAGAGGAAAGTAAAGGGAAGATCCTGGGCTCAATTTGTGTGCTGCGCATCTGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

24.33

Weight (kDa)

8.28

Isoelectric Point (pI)

59.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 658
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 148
AciI CCGC 1 cut(s) 23
AclWI GGATC 1 cut(s) 627
AcoI YGGCCR 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 57, 578
AfaI GTAC 2 cut(s) 122, 228
AfiI CCNNNNNNNGG 4 cut(s) 182, 183, 184, 394
AgsI TTSAA 4 cut(s) 107, 173, 212, 382
AjnI CCWGG 1 cut(s) 635
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 41, 200, 476
AluI AGCT 3 cut(s) 41, 200, 476
Alw21I GWGCWC 2 cut(s) 359, 512
AlwI GGATC 1 cut(s) 627
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 2 cut(s) 399, 581
ApeKI GCWGC 3 cut(s) 476, 545, 654
ArsI GACNNNNNNTTYG 2 cut(s) 427, 459
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 491
AsuHPI GGTGA 1 cut(s) 355
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 1 cut(s) 491
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 236, 643
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 2 cut(s) 359, 512
BbvI GCAGC 3 cut(s) 463, 532, 641
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 637
BclI TGATCA 1 cut(s) 190
BfmI CTRYAG 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 3 cut(s) 477, 546, 655
BlsI GCNGC 3 cut(s) 478, 547, 656
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 1 cut(s) 637
Bme18I GGWCC 1 cut(s) 491
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 637
BmrI ACTGGG 3 cut(s) 55, 276, 369
BmsI GCATC 3 cut(s) 22, 263, 668
BmuI ACTGGG 3 cut(s) 55, 276, 369
BpiI GAAGAC 1 cut(s) 57
Bpu10I CCTNAGC 2 cut(s) 339, 459
BpuEI CTTGAG 1 cut(s) 388
BsaJI CCNNGG 2 cut(s) 178, 636
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 183, 184, 394
Bse118I RCCGGY 1 cut(s) 579
Bse1I ACTGG 5 cut(s) 61, 208, 271, 364, 541
Bse3DI GCAATG 1 cut(s) 333
BseBI CCWGG 1 cut(s) 637
BseDI CCNNGG 2 cut(s) 178, 636
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 4 cut(s) 182, 183, 184, 394
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 5 cut(s) 61, 208, 271, 364, 541
BseXI GCAGC 3 cut(s) 463, 532, 641
BshFI GGCC 2 cut(s) 401, 583
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 359, 512
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 580
BslI CCNNNNNNNGG 4 cut(s) 182, 183, 184, 394
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 401, 583
BsoBI CYCGRG 1 cut(s) 177
Bsp1286I GDGCHC 4 cut(s) 236, 359, 512, 643
Bsp143I GATC 3 cut(s) 190, 330, 632
BspACI CCGC 1 cut(s) 23
BspANI GGCC 2 cut(s) 401, 583
BspCNI CTCAG 1 cut(s) 451
BspHI TCATGA 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 150
BspMAI CTGCAG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 1 cut(s) 627
BspT107I GGYRCC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 579
BsrI ACTGG 5 cut(s) 61, 208, 271, 364, 541
BssAI RCCGGY 1 cut(s) 579
BssECI CCNNGG 2 cut(s) 178, 636
BssMI GATC 3 cut(s) 190, 330, 632
Bst2UI CCWGG 1 cut(s) 637
Bst4CI ACNGT 2 cut(s) 83, 505
Bst6I CTCTTC 2 cut(s) 321, 608
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 3 cut(s) 339, 459, 549
BstF5I GGATG 1 cut(s) 193
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 3 cut(s) 193, 333, 635
BstMBI GATC 3 cut(s) 190, 330, 632
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstNI CCWGG 1 cut(s) 637
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 635
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 463, 532, 641
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 632
BstYI RGATCY 1 cut(s) 632
BsuRI GGCC 2 cut(s) 401, 583
BtsCI GGATG 1 cut(s) 193
BtsIMutI CAGTG 4 cut(s) 357, 425, 493, 510
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 581
CciI TCATGA 1 cut(s) 159
CfoI GCGC 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 579
Cfr13I GGNCC 1 cut(s) 491
Csp6I GTAC 2 cut(s) 121, 227
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CviAII CATG 4 cut(s) 5, 160, 283, 290
CviQI GTAC 2 cut(s) 121, 227
DdeI CTNAG 3 cut(s) 339, 459, 549
DpnI GATC 3 cut(s) 192, 332, 634
DpnII GATC 3 cut(s) 190, 330, 632
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 2 cut(s) 321, 608
EarI CTCTTC 2 cut(s) 321, 608
Eco147I AGGCCT 1 cut(s) 401
Eco24I GRGCYC 2 cut(s) 236, 643
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 491
Eco57I CTGAAG 2 cut(s) 57, 578
Eco88I CYCGRG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 635
EcoRV GATATC 1 cut(s) 589
EcoT22I ATGCAT 2 cut(s) 6, 57
EcoT38I GRGCYC 2 cut(s) 236, 643
FaeI CATG 4 cut(s) 8, 163, 286, 293
FaiI YATR 7 cut(s) 6, 51, 53, 140, 161, 284, 291
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FatI CATG 4 cut(s) 4, 159, 282, 289
FauI CCCGC 1 cut(s) 30
FauNDI CATATG 1 cut(s) 51
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 3 cut(s) 477, 546, 655
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 2 cut(s) 236, 643
Fsp4HI GCNGC 3 cut(s) 477, 546, 655
FspI TGCGCA 1 cut(s) 658
GlaI GCGC 1 cut(s) 658
GluI GCNGC 3 cut(s) 477, 546, 655
HaeIII GGCC 2 cut(s) 401, 583
HapII CCGG 1 cut(s) 580
HhaI GCGC 1 cut(s) 659
Hin1II CATG 4 cut(s) 8, 163, 286, 293
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HinfI GANTC 6 cut(s) 115, 286, 302, 514, 520, 527
HpaII CCGG 1 cut(s) 580
HphI GGTGA 1 cut(s) 355
Hpy188I TCNGA 4 cut(s) 31, 136, 519, 597
Hpy188III TCNNGA 4 cut(s) 160, 217, 458, 524
HpyAV CCTTC 1 cut(s) 602
HpyCH4III ACNGT 2 cut(s) 83, 505
HpyCH4V TGCA 6 cut(s) 4, 55, 224, 254, 479, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 3 cut(s) 339, 459, 549
Hsp92II CATG 4 cut(s) 8, 163, 286, 293
HspAI GCGC 1 cut(s) 657
KroI GCCGGC 1 cut(s) 579
KroNI GCCGGC 1 cut(s) 581
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 3 cut(s) 190, 330, 632
LmnI GCTCC 3 cut(s) 354, 454, 460
Lsp1109I GCAGC 3 cut(s) 463, 532, 641
LweI GCATC 3 cut(s) 22, 263, 668
MaeIII GTNAC 3 cut(s) 83, 343, 496
MalI GATC 3 cut(s) 192, 332, 634
MboI GATC 3 cut(s) 190, 330, 632
MboII GAAGA 7 cut(s) 57, 86, 249, 331, 338, 625, 642
MflI RGATCY 1 cut(s) 632
MhlI GDGCHC 4 cut(s) 236, 359, 512, 643
MluCI AATT 2 cut(s) 644, 667
MlyI GAGTC 2 cut(s) 280, 311
MnlI CCTC 7 cut(s) 139, 173, 289, 391, 412, 600, 609
Mph1103I ATGCAT 2 cut(s) 6, 57
MroNI GCCGGC 1 cut(s) 579
MseI TTAA 1 cut(s) 89
MspI CCGG 1 cut(s) 580
MspR9I CCNGG 1 cut(s) 637
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 637
MwoI GCNNNNNNNGC 1 cut(s) 52
NaeI GCCGGC 1 cut(s) 581
NdeI CATATG 1 cut(s) 51
NdeII GATC 3 cut(s) 190, 330, 632
NgoMIV GCCGGC 1 cut(s) 579
NlaIII CATG 4 cut(s) 8, 163, 286, 293
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 83, 343
NsbI TGCGCA 1 cut(s) 658
NsiI ATGCAT 2 cut(s) 6, 57
NspI RCATGY 1 cut(s) 8
PagI TCATGA 1 cut(s) 159
PceI AGGCCT 1 cut(s) 401
PctI GAATGC 1 cut(s) 55
PdiI GCCGGC 1 cut(s) 581
PfeI GAWTC 4 cut(s) 115, 514, 520, 527
PkrI GCNGC 3 cut(s) 478, 547, 656
PleI GAGTC 2 cut(s) 280, 310
PpsI GAGTC 2 cut(s) 280, 310
Psp6I CCWGG 1 cut(s) 635
PspGI CCWGG 1 cut(s) 635
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 491
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 632
RsaI GTAC 2 cut(s) 122, 228
RsaNI GTAC 2 cut(s) 121, 227
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 3 cut(s) 477, 546, 655
Sau3AI GATC 3 cut(s) 190, 330, 632
Sau96I GGNCC 1 cut(s) 491
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 2 cut(s) 280, 311
ScrFI CCNGG 1 cut(s) 637
SduI GDGCHC 4 cut(s) 236, 359, 512, 643
SetI ASST 7 cut(s) 43, 184, 202, 253, 345, 478, 486
SfaNI GCATC 3 cut(s) 22, 263, 668
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 2 cut(s) 644, 667
SseBI AGGCCT 1 cut(s) 401
SsiI CCGC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 635
TaaI ACNGT 2 cut(s) 83, 505
TasI AATT 2 cut(s) 644, 667
TatI WGTACW 2 cut(s) 120, 226
TfiI GAWTC 4 cut(s) 115, 514, 520, 527
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 4 cut(s) 364, 432, 493, 510
TseFI GTSAC 2 cut(s) 83, 343
TseI GCWGC 3 cut(s) 476, 545, 654
Tsp45I GTSAC 2 cut(s) 83, 343
TspDTI ATGAA 3 cut(s) 57, 148, 339
TspRI CASTG 4 cut(s) 364, 432, 493, 510
VpaK11BI GGWCC 1 cut(s) 491
XceI RCATGY 1 cut(s) 8
ZrmI AGTACT 1 cut(s) 122
Zsp2I ATGCAT 2 cut(s) 6, 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.