endonuclease activity
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 150 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1690, 2743 |
| Acc36I | ACCTGC | 1 cut(s) | 3502 |
| AccB1I | GGYRCC | 4 cut(s) | 161, 416, 722, 3391 |
| AccII | CGCG | 2 cut(s) | 114, 116 |
| AcoI | YGGCCR | 1 cut(s) | 3834 |
| AcsI | RAATTY | 5 cut(s) | 2160, 2317, 2666, 2851, 2874 |
| AcyI | GRCGYC | 1 cut(s) | 162 |
| AdeI | CACNNNGTG | 1 cut(s) | 55 |
| AfaI | GTAC | 3 cut(s) | 622, 1128, 3471 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 1381, 1388, 3425, 3426, 3427, 3637 |
| AflIII | ACRYGT | 4 cut(s) | 1000, 1123, 1129, 1156 |
| AjnI | CCWGG | 3 cut(s) | 540, 3031, 3887 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3617, 3649 |
| AleI | CACNNNNGTG | 1 cut(s) | 96 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 577, 609 |
| Alw21I | GWGCWC | 4 cut(s) | 28, 178, 3602, 3764 |
| Alw26I | GTCTC | 7 cut(s) | 193, 1348, 1442, 1692, 1851, 2067, 3803 |
| Alw44I | GTGCAC | 1 cut(s) | 174 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 326, 2333 |
| Ama87I | CYCGRG | 5 cut(s) | 94, 388, 2741, 3367, 3646 |
| AoxI | GGCC | 3 cut(s) | 74, 3642, 3834 |
| ApaLI | GTGCAC | 1 cut(s) | 174 |
| ApoI | RAATTY | 5 cut(s) | 2160, 2317, 2666, 2851, 2874 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3670, 3702 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 915, 1307, 2715 |
| AspA2I | CCTAGG | 1 cut(s) | 3421 |
| AspLEI | GCGC | 4 cut(s) | 116, 118, 164, 3911 |
| AspS9I | GGNCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| AsuC2I | CCSGG | 4 cut(s) | 231, 389, 390, 3552 |
| AsuII | TTCGAA | 1 cut(s) | 2849 |
| AsuNHI | GCTAGC | 1 cut(s) | 1370 |
| AvaI | CYCGRG | 5 cut(s) | 94, 388, 2741, 3367, 3646 |
| AvaII | GGWCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| AvrII | CCTAGG | 1 cut(s) | 3421 |
| BaeGI | GKGCMC | 3 cut(s) | 42, 178, 3895 |
| BanI | GGYRCC | 4 cut(s) | 161, 416, 722, 3391 |
| BanII | GRGCYC | 3 cut(s) | 28, 1498, 3764 |
| BauI | CACGAG | 1 cut(s) | 41 |
| BbsI | GAAGAC | 2 cut(s) | 497, 2739 |
| Bbv12I | GWGCWC | 4 cut(s) | 28, 178, 3602, 3764 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 155, 189, 2732, 2766 |
| BciT130I | CCWGG | 3 cut(s) | 542, 3033, 3889 |
| BclI | TGATCA | 3 cut(s) | 763, 2143, 3433 |
| BcnI | CCSGG | 4 cut(s) | 231, 389, 390, 3552 |
| BcoDI | GTCTC | 7 cut(s) | 193, 1348, 1442, 1692, 1851, 2067, 3803 |
| BfaI | CTAG | 6 cut(s) | 102, 803, 1371, 2802, 2856, 3422 |
| BfmI | CTRYAG | 8 cut(s) | 1719, 1746, 1878, 1905, 2077, 2121, 2529, 3465 |
| BfoI | RGCGCY | 1 cut(s) | 165 |
| BfuAI | ACCTGC | 1 cut(s) | 3502 |
| BglII | AGATCT | 2 cut(s) | 13, 2525 |
| BlnI | CCTAGG | 1 cut(s) | 3421 |
| Bme1390I | CCNGG | 7 cut(s) | 231, 389, 390, 542, 3033, 3552, 3889 |
| Bme18I | GGWCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| BmeT110I | CYCGRG | 5 cut(s) | 94, 388, 2741, 3367, 3646 |
| BmgT120I | GGNCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| BmiI | GGNNCC | 7 cut(s) | 136, 163, 418, 724, 1495, 2092, 3393 |
| BmrFI | CCNGG | 7 cut(s) | 231, 389, 390, 542, 3033, 3552, 3889 |
| BmrI | ACTGGG | 4 cut(s) | 2469, 3250, 3519, 3612 |
| BmtI | GCTAGC | 1 cut(s) | 1374 |
| BmuI | ACTGGG | 4 cut(s) | 2469, 3250, 3519, 3612 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1936 |
| BpiI | GAAGAC | 2 cut(s) | 497, 2739 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 191, 223, 842, 874 |
| BpmI | CTGGAG | 5 cut(s) | 387, 1707, 1716, 1875, 2091 |
| Bpu14I | TTCGAA | 1 cut(s) | 2849 |
| BpuEI | CTTGAG | 1 cut(s) | 2580 |
| BpuMI | CCSGG | 4 cut(s) | 231, 389, 390, 3552 |
| BsaBI | GATNNNNATC | 1 cut(s) | 216 |
| BsaHI | GRCGYC | 1 cut(s) | 162 |
| BsaI | GGTCTC | 3 cut(s) | 1442, 1692, 1851 |
| BsaWI | WCCGGW | 2 cut(s) | 1381, 1802 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1323, 1353 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 1381, 1388, 3425, 3426, 3427, 3637 |
| Bse118I | RCCGGY | 2 cut(s) | 2951, 3832 |
| Bse3DI | GCAATG | 5 cut(s) | 664, 2929, 3075, 3576, 3735 |
| Bse8I | GATNNNNATC | 1 cut(s) | 216 |
| BseBI | CCWGG | 3 cut(s) | 542, 3033, 3889 |
| BseGI | GGATG | 7 cut(s) | 388, 775, 1510, 2296, 2602, 2811, 3436 |
| BseJI | GATNNNNATC | 1 cut(s) | 216 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 1381, 1388, 3425, 3426, 3427, 3637 |
| BseMI | GCAATG | 5 cut(s) | 664, 2929, 3075, 3576, 3735 |
| BseMII | CTCAG | 9 cut(s) | 31, 333, 1078, 1437, 1532, 1653, 2028, 3267, 3722 |
| BsePI | GCGCGC | 1 cut(s) | 114 |
| BseRI | GAGGAG | 1 cut(s) | 3696 |
| BseSI | GKGCMC | 3 cut(s) | 42, 178, 3895 |
| BseYI | CCCAGC | 1 cut(s) | 2963 |
| Bsh1236I | CGCG | 2 cut(s) | 114, 116 |
| BshFI | GGCC | 3 cut(s) | 76, 3644, 3836 |
| BshNI | GGYRCC | 4 cut(s) | 161, 416, 722, 3391 |
| BsiHKAI | GWGCWC | 4 cut(s) | 28, 178, 3602, 3764 |
| BsiHKCI | CYCGRG | 5 cut(s) | 94, 388, 2741, 3367, 3646 |
| BsiSI | CCGG | 7 cut(s) | 231, 389, 1382, 1803, 2952, 3551, 3833 |
| BslFI | GGGAC | 3 cut(s) | 613, 959, 1696 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 1381, 1388, 3425, 3426, 3427, 3637 |
| BsmAI | GTCTC | 7 cut(s) | 193, 1348, 1442, 1692, 1851, 2067, 3803 |
| BsmFI | GGGAC | 3 cut(s) | 613, 959, 1696 |
| BsmI | GAATGC | 3 cut(s) | 439, 1117, 3298 |
| BsnI | GGCC | 3 cut(s) | 76, 3644, 3836 |
| Bso31I | GGTCTC | 3 cut(s) | 1442, 1692, 1851 |
| BsoBI | CYCGRG | 5 cut(s) | 94, 388, 2741, 3367, 3646 |
| Bsp119I | TTCGAA | 1 cut(s) | 2849 |
| Bsp1286I | GDGCHC | 7 cut(s) | 28, 42, 178, 1498, 3602, 3764, 3895 |
| Bsp1407I | TGTACA | 1 cut(s) | 1126 |
| Bsp19I | CCATGG | 1 cut(s) | 3016 |
| BspANI | GGCC | 3 cut(s) | 76, 3644, 3836 |
| BspCNI | CTCAG | 9 cut(s) | 30, 332, 1077, 1438, 1531, 1652, 2027, 3266, 3721 |
| BspFNI | CGCG | 2 cut(s) | 114, 116 |
| BspLI | GGNNCC | 7 cut(s) | 136, 163, 418, 724, 1495, 2092, 3393 |
| BspMAI | CTGCAG | 3 cut(s) | 1723, 1882, 3469 |
| BspMI | ACCTGC | 1 cut(s) | 3502 |
| BspOI | GCTAGC | 1 cut(s) | 1374 |
| BspT104I | TTCGAA | 1 cut(s) | 2849 |
| BspT107I | GGYRCC | 4 cut(s) | 161, 416, 722, 3391 |
| BspTNI | GGTCTC | 3 cut(s) | 1442, 1692, 1851 |
| BsrDI | GCAATG | 5 cut(s) | 664, 2929, 3075, 3576, 3735 |
| BsrFI | RCCGGY | 2 cut(s) | 2951, 3832 |
| BsrGI | TGTACA | 1 cut(s) | 1126 |
| BssAI | RCCGGY | 2 cut(s) | 2951, 3832 |
| BssHII | GCGCGC | 1 cut(s) | 114 |
| BssNI | GRCGYC | 1 cut(s) | 162 |
| BssSI | CACGAG | 1 cut(s) | 41 |
| BssT1I | CCWWGG | 3 cut(s) | 3016, 3421, 3582 |
| Bst2BI | CACGAG | 1 cut(s) | 41 |
| Bst2UI | CCWGG | 3 cut(s) | 542, 3033, 3889 |
| Bst6I | CTCTTC | 5 cut(s) | 1101, 1351, 2996, 3564, 3860 |
| BstACI | GRCGYC | 1 cut(s) | 162 |
| BstAUI | TGTACA | 1 cut(s) | 1126 |
| BstBI | TTCGAA | 1 cut(s) | 2849 |
| BstC8I | GCNNGC | 6 cut(s) | 116, 160, 1372, 1400, 2920, 3834 |
| BstDSI | CCRYGG | 1 cut(s) | 3016 |
| BstEII | GGTNACC | 1 cut(s) | 2471 |
| BstF5I | GGATG | 7 cut(s) | 388, 775, 1510, 2296, 2602, 2811, 3436 |
| BstFNI | CGCG | 2 cut(s) | 114, 116 |
| BstH2I | RGCGCY | 1 cut(s) | 165 |
| BstHHI | GCGC | 4 cut(s) | 116, 118, 164, 3911 |
| BstMAI | GTCTC | 7 cut(s) | 193, 1348, 1442, 1692, 1851, 2067, 3803 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 704, 1141, 1613, 1988, 2606, 2957, 3295 |
| BstNI | CCWGG | 3 cut(s) | 542, 3033, 3889 |
| BstNSI | RCATGY | 4 cut(s) | 1004, 1127, 1133, 1160 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1936 |
| BstPI | GGTNACC | 1 cut(s) | 2471 |
| BstSCI | CCNGG | 7 cut(s) | 229, 387, 388, 540, 3031, 3550, 3887 |
| BstSFI | CTRYAG | 8 cut(s) | 1719, 1746, 1878, 1905, 2077, 2121, 2529, 3465 |
| BstSLI | GKGCMC | 3 cut(s) | 42, 178, 3895 |
| BstUI | CGCG | 2 cut(s) | 114, 116 |
| BstV2I | GAAGAC | 2 cut(s) | 497, 2739 |
| BstX2I | RGATCY | 9 cut(s) | 13, 217, 290, 1384, 2329, 2525, 3105, 3245, 3884 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 455, 1643, 2018, 2566 |
| BstYI | RGATCY | 9 cut(s) | 13, 217, 290, 1384, 2329, 2525, 3105, 3245, 3884 |
| BsuRI | GGCC | 3 cut(s) | 76, 3644, 3836 |
| BtgI | CCRYGG | 1 cut(s) | 3016 |
| BtsCI | GGATG | 7 cut(s) | 388, 775, 1510, 2296, 2602, 2811, 3436 |
| BtsI | GCAGTG | 1 cut(s) | 1342 |
| BtsIMutI | CAGTG | 9 cut(s) | 726, 1342, 1615, 2206, 2830, 3600, 3668, 3742, 3762 |
| BveI | ACCTGC | 1 cut(s) | 3502 |
| Cac8I | GCNNGC | 6 cut(s) | 116, 160, 1372, 1400, 2920, 3834 |
| CaiI | CAGNNNCTG | 2 cut(s) | 326, 2333 |
| CfoI | GCGC | 4 cut(s) | 116, 118, 164, 3911 |
| Cfr10I | RCCGGY | 2 cut(s) | 2951, 3832 |
| Cfr13I | GGNCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| Cfr9I | CCCGGG | 1 cut(s) | 388 |
| CpoI | CGGWCCG | 1 cut(s) | 233 |
| CseI | GACGC | 1 cut(s) | 120 |
| Csp6I | GTAC | 3 cut(s) | 621, 1127, 3470 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 3183, 3218, 3494, 3529 |
| CspI | CGGWCCG | 1 cut(s) | 233 |
| CviQI | GTAC | 3 cut(s) | 621, 1127, 3470 |
| DinI | GGCGCC | 1 cut(s) | 163 |
| DraI | TTTAAA | 1 cut(s) | 1468 |
| DraIII | CACNNNGTG | 1 cut(s) | 55 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1690, 2743 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1690, 2743 |
| EaeI | YGGCCR | 1 cut(s) | 3834 |
| Eam1104I | CTCTTC | 5 cut(s) | 1101, 1351, 2996, 3564, 3860 |
| EarI | CTCTTC | 5 cut(s) | 1101, 1351, 2996, 3564, 3860 |
| EciI | GGCGGA | 1 cut(s) | 291 |
| Ecl136II | GAGCTC | 2 cut(s) | 26, 3762 |
| Eco130I | CCWWGG | 3 cut(s) | 3016, 3421, 3582 |
| Eco147I | AGGCCT | 1 cut(s) | 3644 |
| Eco24I | GRGCYC | 3 cut(s) | 28, 1498, 3764 |
| Eco31I | GGTCTC | 3 cut(s) | 1442, 1692, 1851 |
| Eco32I | GATATC | 1 cut(s) | 3842 |
| Eco47I | GGWCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| Eco53kI | GAGCTC | 2 cut(s) | 26, 3762 |
| Eco88I | CYCGRG | 5 cut(s) | 94, 388, 2741, 3367, 3646 |
| Eco91I | GGTNACC | 1 cut(s) | 2471 |
| EcoICRI | GAGCTC | 2 cut(s) | 26, 3762 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2090 |
| EcoO65I | GGTNACC | 1 cut(s) | 2471 |
| EcoRI | GAATTC | 1 cut(s) | 2851 |
| EcoRII | CCWGG | 3 cut(s) | 540, 3031, 3887 |
| EcoRV | GATATC | 1 cut(s) | 3842 |
| EcoT14I | CCWWGG | 3 cut(s) | 3016, 3421, 3582 |
| EcoT22I | ATGCAT | 5 cut(s) | 475, 700, 957, 1359, 2500 |
| EcoT38I | GRGCYC | 3 cut(s) | 28, 1498, 3764 |
| EgeI | GGCGCC | 1 cut(s) | 163 |
| EheI | GGCGCC | 1 cut(s) | 163 |
| ErhI | CCWWGG | 3 cut(s) | 3016, 3421, 3582 |
| FaqI | GGGAC | 3 cut(s) | 613, 959, 1696 |
| FauI | CCCGC | 4 cut(s) | 62, 96, 2101, 2602 |
| FbaI | TGATCA | 3 cut(s) | 763, 2143, 3433 |
| FokI | GGATG | 7 cut(s) | 395, 782, 1517, 2303, 2589, 2818, 3443 |
| FriOI | GRGCYC | 3 cut(s) | 28, 1498, 3764 |
| FspBI | CTAG | 6 cut(s) | 102, 803, 1371, 2802, 2856, 3422 |
| GlaI | GCGC | 4 cut(s) | 115, 117, 163, 3910 |
| GsaI | CCCAGC | 1 cut(s) | 2967 |
| GsuI | CTGGAG | 5 cut(s) | 387, 1707, 1716, 1875, 2091 |
| HaeII | RGCGCY | 1 cut(s) | 165 |
| HaeIII | GGCC | 3 cut(s) | 76, 3644, 3836 |
| HapII | CCGG | 7 cut(s) | 231, 389, 1382, 1803, 2952, 3551, 3833 |
| HgaI | GACGC | 1 cut(s) | 120 |
| HhaI | GCGC | 4 cut(s) | 116, 118, 164, 3911 |
| Hin1I | GRCGYC | 1 cut(s) | 162 |
| Hin6I | GCGC | 4 cut(s) | 114, 116, 162, 3909 |
| HinP1I | GCGC | 4 cut(s) | 114, 116, 162, 3909 |
| HincII | GTYRAC | 1 cut(s) | 538 |
| HindII | GTYRAC | 1 cut(s) | 538 |
| HpaII | CCGG | 7 cut(s) | 231, 389, 1382, 1803, 2952, 3551, 3833 |
| Hpy166II | GTNNAC | 7 cut(s) | 176, 538, 665, 1547, 1597, 1972, 3205 |
| Hpy8I | GTNNAC | 7 cut(s) | 176, 538, 665, 1547, 1597, 1972, 3205 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 704, 1141, 1613, 1988, 2606, 2957, 3295 |
| Hsp92I | GRCGYC | 1 cut(s) | 162 |
| HspAI | GCGC | 4 cut(s) | 114, 116, 162, 3909 |
| KasI | GGCGCC | 1 cut(s) | 161 |
| KroI | GCCGGC | 1 cut(s) | 3832 |
| KroNI | GCCGGC | 1 cut(s) | 3834 |
| Ksp22I | TGATCA | 3 cut(s) | 763, 2143, 3433 |
| MaeI | CTAG | 6 cut(s) | 102, 803, 1371, 2802, 2856, 3422 |
| MfeI | CAATTG | 1 cut(s) | 3195 |
| MflI | RGATCY | 9 cut(s) | 13, 217, 290, 1384, 2329, 2525, 3105, 3245, 3884 |
| MhlI | GDGCHC | 7 cut(s) | 28, 42, 178, 1498, 3602, 3764, 3895 |
| Mly113I | GGCGCC | 1 cut(s) | 162 |
| MmeI | TCCRAC | 1 cut(s) | 2613 |
| Mph1103I | ATGCAT | 5 cut(s) | 475, 700, 957, 1359, 2500 |
| MroNI | GCCGGC | 1 cut(s) | 3832 |
| MroXI | GAANNNNTTC | 3 cut(s) | 915, 1307, 2715 |
| MseI | TTAA | 8 cut(s) | 297, 527, 675, 711, 752, 1410, 1467, 2379 |
| MspA1I | CMGCKG | 2 cut(s) | 2141, 2609 |
| MspI | CCGG | 7 cut(s) | 231, 389, 1382, 1803, 2952, 3551, 3833 |
| MspR9I | CCNGG | 7 cut(s) | 231, 389, 390, 542, 3033, 3552, 3889 |
| MunI | CAATTG | 1 cut(s) | 3195 |
| Mva1269I | GAATGC | 3 cut(s) | 439, 1117, 3298 |
| MvaI | CCWGG | 3 cut(s) | 542, 3033, 3889 |
| MvnI | CGCG | 2 cut(s) | 114, 116 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 704, 1141, 1613, 1988, 2606, 2957, 3295 |
| NaeI | GCCGGC | 1 cut(s) | 3834 |
| NarI | GGCGCC | 1 cut(s) | 162 |
| NciI | CCSGG | 4 cut(s) | 231, 389, 390, 3552 |
| NcoI | CCATGG | 1 cut(s) | 3016 |
| NgoMIV | GCCGGC | 1 cut(s) | 3832 |
| NheI | GCTAGC | 1 cut(s) | 1370 |
| NlaIV | GGNNCC | 7 cut(s) | 136, 163, 418, 724, 1495, 2092, 3393 |
| NmeAIII | GCCGAG | 2 cut(s) | 190, 1368 |
| NsiI | ATGCAT | 5 cut(s) | 475, 700, 957, 1359, 2500 |
| NspI | RCATGY | 4 cut(s) | 1004, 1127, 1133, 1160 |
| NspV | TTCGAA | 1 cut(s) | 2849 |
| OliI | CACNNNNGTG | 1 cut(s) | 96 |
| PaeR7I | CTCGAG | 1 cut(s) | 3646 |
| PauI | GCGCGC | 1 cut(s) | 114 |
| PceI | AGGCCT | 1 cut(s) | 3644 |
| PciI | ACATGT | 4 cut(s) | 1000, 1123, 1129, 1156 |
| PctI | GAATGC | 3 cut(s) | 439, 1117, 3298 |
| PdiI | GCCGGC | 1 cut(s) | 3834 |
| PdmI | GAANNNNTTC | 3 cut(s) | 915, 1307, 2715 |
| PflFI | GACNNNGTC | 1 cut(s) | 3167 |
| PluTI | GGCGCC | 1 cut(s) | 165 |
| PpuMI | RGGWCCY | 1 cut(s) | 2090 |
| PscI | ACATGT | 4 cut(s) | 1000, 1123, 1129, 1156 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1936 |
| PsiI | TTATAA | 1 cut(s) | 150 |
| Psp124BI | GAGCTC | 2 cut(s) | 28, 3764 |
| Psp5II | RGGWCCY | 1 cut(s) | 2090 |
| Psp6I | CCWGG | 3 cut(s) | 540, 3031, 3887 |
| PspEI | GGTNACC | 1 cut(s) | 2471 |
| PspFI | CCCAGC | 1 cut(s) | 2963 |
| PspGI | CCWGG | 3 cut(s) | 540, 3031, 3887 |
| PspN4I | GGNNCC | 7 cut(s) | 136, 163, 418, 724, 1495, 2092, 3393 |
| PspPI | GGNCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| PspPPI | RGGWCCY | 1 cut(s) | 2090 |
| PspXI | VCTCGAGB | 1 cut(s) | 3646 |
| PstI | CTGCAG | 3 cut(s) | 1723, 1882, 3469 |
| PstNI | CAGNNNCTG | 2 cut(s) | 326, 2333 |
| PsuI | RGATCY | 9 cut(s) | 13, 217, 290, 1384, 2329, 2525, 3105, 3245, 3884 |
| PsyI | GACNNNGTC | 1 cut(s) | 3167 |
| PteI | GCGCGC | 1 cut(s) | 114 |
| PvuII | CAGCTG | 1 cut(s) | 2141 |
| RsaI | GTAC | 3 cut(s) | 622, 1128, 3471 |
| RsaNI | GTAC | 3 cut(s) | 621, 1127, 3470 |
| Rsr2I | CGGWCCG | 1 cut(s) | 233 |
| RsrII | CGGWCCG | 1 cut(s) | 233 |
| SacI | GAGCTC | 2 cut(s) | 28, 3764 |
| SaqAI | TTAA | 8 cut(s) | 297, 527, 675, 711, 752, 1410, 1467, 2379 |
| Sau96I | GGNCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| ScrFI | CCNGG | 7 cut(s) | 231, 389, 390, 542, 3033, 3552, 3889 |
| SduI | GDGCHC | 7 cut(s) | 28, 42, 178, 1498, 3602, 3764, 3895 |
| SfcI | CTRYAG | 8 cut(s) | 1719, 1746, 1878, 1905, 2077, 2121, 2529, 3465 |
| SfoI | GGCGCC | 1 cut(s) | 163 |
| Sfr274I | CTCGAG | 1 cut(s) | 3646 |
| SfuI | TTCGAA | 1 cut(s) | 2849 |
| SinI | GGWCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| SlaI | CTCGAG | 1 cut(s) | 3646 |
| SmaI | CCCGGG | 1 cut(s) | 390 |
| SmlI | CTYRAG | 2 cut(s) | 2595, 3646 |
| SmoI | CTYRAG | 2 cut(s) | 2595, 3646 |
| SseBI | AGGCCT | 1 cut(s) | 3644 |
| SspDI | GGCGCC | 1 cut(s) | 161 |
| SspI | AATATT | 1 cut(s) | 2716 |
| SspMI | CTAG | 6 cut(s) | 102, 803, 1371, 2802, 2856, 3422 |
| SstI | GAGCTC | 2 cut(s) | 28, 3764 |
| StuI | AGGCCT | 1 cut(s) | 3644 |
| StyD4I | CCNGG | 7 cut(s) | 229, 387, 388, 540, 3031, 3550, 3887 |
| StyI | CCWWGG | 3 cut(s) | 3016, 3421, 3582 |
| TaqI | TCGA | 4 cut(s) | 2190, 2849, 3647, 3764 |
| TaqII | GACCGA | 2 cut(s) | 509, 1466 |
| TatI | WGTACW | 2 cut(s) | 1126, 3469 |
| Tru1I | TTAA | 8 cut(s) | 297, 527, 675, 711, 752, 1410, 1467, 2379 |
| Tru9I | TTAA | 8 cut(s) | 297, 527, 675, 711, 752, 1410, 1467, 2379 |
| TscAI | CASTG | 9 cut(s) | 733, 1342, 1615, 2206, 2830, 3607, 3675, 3742, 3762 |
| TspGWI | ACGGA | 2 cut(s) | 432, 3675 |
| TspMI | CCCGGG | 1 cut(s) | 388 |
| TspRI | CASTG | 9 cut(s) | 733, 1342, 1615, 2206, 2830, 3607, 3675, 3742, 3762 |
| Tth111I | GACNNNGTC | 1 cut(s) | 3167 |
| VneI | GTGCAC | 1 cut(s) | 174 |
| VpaK11BI | GGWCC | 6 cut(s) | 233, 1646, 2021, 2090, 3013, 3740 |
| XapI | RAATTY | 5 cut(s) | 2160, 2317, 2666, 2851, 2874 |
| XbaI | TCTAGA | 1 cut(s) | 802 |
| XceI | RCATGY | 4 cut(s) | 1004, 1127, 1133, 1160 |
| XhoI | CTCGAG | 1 cut(s) | 3646 |
| XmaI | CCCGGG | 1 cut(s) | 388 |
| XmaJI | CCTAGG | 1 cut(s) | 3421 |
| XmnI | GAANNNNTTC | 3 cut(s) | 915, 1307, 2715 |
| XspI | CTAG | 6 cut(s) | 102, 803, 1371, 2802, 2856, 3422 |
| Zsp2I | ATGCAT | 5 cut(s) | 475, 700, 957, 1359, 2500 |