endonuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 631 |
| Acc16I | TGCGCA | 1 cut(s) | 1602 |
| Acc36I | ACCTGC | 1 cut(s) | 190 |
| AccB1I | GGYRCC | 1 cut(s) | 1174 |
| AccII | CGCG | 1 cut(s) | 1525 |
| AciI | CCGC | 3 cut(s) | 894, 914, 1525 |
| AclWI | GGATC | 2 cut(s) | 595, 1571 |
| AcsI | RAATTY | 2 cut(s) | 249, 554 |
| AcuI | CTGAAG | 3 cut(s) | 906, 1083, 1524 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 515, 1208, 1209, 1210, 1336 |
| AjnI | CCWGG | 3 cut(s) | 565, 832, 1579 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 1011, 1043, 1133, 1165, 1316, 1348 |
| Alw21I | GWGCWC | 2 cut(s) | 1301, 1457 |
| Alw26I | GTCTC | 1 cut(s) | 156 |
| AlwI | GGATC | 2 cut(s) | 595, 1571 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 158 |
| Ama87I | CYCGRG | 1 cut(s) | 1203 |
| AoxI | GGCC | 2 cut(s) | 1341, 1527 |
| ApeKI | GCWGC | 8 cut(s) | 20, 68, 260, 677, 761, 1418, 1490, 1598 |
| ApoI | RAATTY | 2 cut(s) | 249, 554 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1369, 1401 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 603 |
| AspLEI | GCGC | 1 cut(s) | 1603 |
| AspS9I | GGNCC | 4 cut(s) | 179, 814, 1433, 1527 |
| AsuC2I | CCSGG | 1 cut(s) | 17 |
| AsuHPI | GGTGA | 7 cut(s) | 302, 410, 419, 476, 554, 622, 1297 |
| AvaI | CYCGRG | 1 cut(s) | 1203 |
| AvaII | GGWCC | 3 cut(s) | 179, 814, 1433 |
| BanI | GGYRCC | 1 cut(s) | 1174 |
| BanII | GRGCYC | 1 cut(s) | 1587 |
| BbsI | GAAGAC | 2 cut(s) | 627, 1083 |
| Bbv12I | GWGCWC | 2 cut(s) | 1301, 1457 |
| BbvI | GCAGC | 8 cut(s) | 7, 55, 272, 689, 748, 1405, 1477, 1585 |
| BccI | CCATC | 4 cut(s) | 125, 137, 455, 1186 |
| BciT130I | CCWGG | 3 cut(s) | 567, 834, 1581 |
| BciVI | GTATCC | 1 cut(s) | 330 |
| BclI | TGATCA | 4 cut(s) | 232, 466, 529, 1216 |
| BcnI | CCSGG | 1 cut(s) | 17 |
| BcoDI | GTCTC | 1 cut(s) | 156 |
| BfaI | CTAG | 3 cut(s) | 92, 327, 924 |
| BfmI | CTRYAG | 4 cut(s) | 166, 183, 210, 1248 |
| BfuAI | ACCTGC | 1 cut(s) | 190 |
| BfuI | GTATCC | 1 cut(s) | 330 |
| BglII | AGATCT | 1 cut(s) | 88 |
| BisI | GCNGC | 9 cut(s) | 21, 69, 261, 678, 762, 895, 1419, 1491, 1599 |
| BlpI | GCTNAGC | 1 cut(s) | 690 |
| BlsI | GCNGC | 9 cut(s) | 22, 70, 262, 679, 763, 896, 1420, 1492, 1600 |
| Bme1390I | CCNGG | 4 cut(s) | 17, 567, 834, 1581 |
| Bme18I | GGWCC | 3 cut(s) | 179, 814, 1433 |
| BmeT110I | CYCGRG | 1 cut(s) | 1203 |
| BmgT120I | GGNCC | 4 cut(s) | 179, 814, 1433, 1527 |
| BmiI | GGNNCC | 3 cut(s) | 655, 898, 1176 |
| BmrFI | CCNGG | 4 cut(s) | 17, 567, 834, 1581 |
| BmrI | ACTGGG | 3 cut(s) | 38, 1081, 1311 |
| BmsI | GCATC | 8 cut(s) | 67, 274, 391, 500, 664, 676, 1036, 1048 |
| BmuI | ACTGGG | 3 cut(s) | 38, 1081, 1311 |
| BpiI | GAAGAC | 2 cut(s) | 627, 1083 |
| BpmI | CTGGAG | 1 cut(s) | 180 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1281, 1401 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 690 |
| BpuEI | CTTGAG | 1 cut(s) | 1330 |
| BpuMI | CCSGG | 1 cut(s) | 17 |
| Bsa29I | ATCGAT | 1 cut(s) | 444 |
| BsaBI | GATNNNNATC | 1 cut(s) | 465 |
| BsaJI | CCNNGG | 4 cut(s) | 817, 833, 1204, 1580 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 515, 1208, 1209, 1210, 1336 |
| Bse118I | RCCGGY | 1 cut(s) | 287 |
| Bse1I | ACTGG | 8 cut(s) | 44, 72, 655, 753, 1087, 1234, 1306, 1486 |
| Bse3DI | GCAATG | 3 cut(s) | 730, 876, 1275 |
| Bse8I | GATNNNNATC | 1 cut(s) | 465 |
| BseBI | CCWGG | 3 cut(s) | 567, 834, 1581 |
| BseCI | ATCGAT | 1 cut(s) | 444 |
| BseDI | CCNNGG | 4 cut(s) | 817, 833, 1204, 1580 |
| BseGI | GGATG | 2 cut(s) | 121, 1219 |
| BseJI | GATNNNNATC | 1 cut(s) | 465 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 515, 1208, 1209, 1210, 1336 |
| BseMI | GCAATG | 3 cut(s) | 730, 876, 1275 |
| BseMII | CTCAG | 5 cut(s) | 117, 621, 704, 783, 1392 |
| BseNI | ACTGG | 8 cut(s) | 44, 72, 655, 753, 1087, 1234, 1306, 1486 |
| BseRI | GAGGAG | 1 cut(s) | 1567 |
| BseXI | GCAGC | 8 cut(s) | 7, 55, 272, 689, 748, 1405, 1477, 1585 |
| BseYI | CCCAGC | 1 cut(s) | 54 |
| BsgI | GTGCAG | 1 cut(s) | 171 |
| Bsh1236I | CGCG | 1 cut(s) | 1525 |
| BshFI | GGCC | 2 cut(s) | 1343, 1529 |
| BshNI | GGYRCC | 1 cut(s) | 1174 |
| BshVI | ATCGAT | 1 cut(s) | 444 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1301, 1457 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1203 |
| BsiSI | CCGG | 2 cut(s) | 17, 288 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 515, 1208, 1209, 1210, 1336 |
| BsmAI | GTCTC | 1 cut(s) | 156 |
| BsmI | GAATGC | 1 cut(s) | 1081 |
| BsnI | GGCC | 2 cut(s) | 1343, 1529 |
| BsoBI | CYCGRG | 1 cut(s) | 1203 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1301, 1457, 1587 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 690 |
| Bsp19I | CCATGG | 1 cut(s) | 817 |
| BspACI | CCGC | 3 cut(s) | 894, 914, 1525 |
| BspANI | GGCC | 2 cut(s) | 1343, 1529 |
| BspCNI | CTCAG | 5 cut(s) | 116, 622, 703, 782, 1393 |
| BspDI | ATCGAT | 1 cut(s) | 444 |
| BspFNI | CGCG | 1 cut(s) | 1525 |
| BspHI | TCATGA | 3 cut(s) | 238, 469, 532 |
| BspLI | GGNNCC | 3 cut(s) | 655, 898, 1176 |
| BspMAI | CTGCAG | 2 cut(s) | 187, 1252 |
| BspMI | ACCTGC | 1 cut(s) | 190 |
| BspPI | GGATC | 2 cut(s) | 595, 1571 |
| BspT107I | GGYRCC | 1 cut(s) | 1174 |
| BsrDI | GCAATG | 3 cut(s) | 730, 876, 1275 |
| BsrFI | RCCGGY | 1 cut(s) | 287 |
| BsrI | ACTGG | 8 cut(s) | 44, 72, 655, 753, 1087, 1234, 1306, 1486 |
| BssAI | RCCGGY | 1 cut(s) | 287 |
| BssECI | CCNNGG | 4 cut(s) | 817, 833, 1204, 1580 |
| BssT1I | CCWWGG | 1 cut(s) | 817 |
| Bst2UI | CCWGG | 3 cut(s) | 567, 834, 1581 |
| Bst4CI | ACNGT | 2 cut(s) | 140, 1450 |
| Bst6I | CTCTTC | 3 cut(s) | 797, 1263, 1552 |
| BstC8I | GCNNGC | 3 cut(s) | 721, 766, 1527 |
| BstDEI | CTNAG | 8 cut(s) | 103, 630, 690, 769, 1281, 1401, 1494, 1548 |
| BstDSI | CCRYGG | 1 cut(s) | 817 |
| BstF5I | GGATG | 2 cut(s) | 121, 1219 |
| BstFNI | CGCG | 1 cut(s) | 1525 |
| BstHHI | GCGC | 1 cut(s) | 1603 |
| BstMAI | GTCTC | 1 cut(s) | 156 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 77, 686, 1078 |
| BstNI | CCWGG | 3 cut(s) | 567, 834, 1581 |
| BstSCI | CCNGG | 4 cut(s) | 15, 565, 832, 1579 |
| BstSFI | CTRYAG | 4 cut(s) | 166, 183, 210, 1248 |
| BstUI | CGCG | 1 cut(s) | 1525 |
| BstV1I | GCAGC | 8 cut(s) | 7, 55, 272, 689, 748, 1405, 1477, 1585 |
| BstV2I | GAAGAC | 2 cut(s) | 627, 1083 |
| BstX2I | RGATCY | 3 cut(s) | 88, 587, 1576 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 107 |
| BstYI | RGATCY | 3 cut(s) | 88, 587, 1576 |
| Bsu15I | ATCGAT | 1 cut(s) | 444 |
| BsuI | GTATCC | 1 cut(s) | 330 |
| BsuRI | GGCC | 2 cut(s) | 1343, 1529 |
| BsuTUI | ATCGAT | 1 cut(s) | 444 |
| BtgI | CCRYGG | 1 cut(s) | 817 |
| BtsCI | GGATG | 2 cut(s) | 121, 1219 |
| BtsIMutI | CAGTG | 6 cut(s) | 79, 760, 1299, 1367, 1435, 1455 |
| BveI | ACCTGC | 1 cut(s) | 190 |
| Cac8I | GCNNGC | 3 cut(s) | 721, 766, 1527 |
| CaiI | CAGNNNCTG | 1 cut(s) | 158 |
| CciI | TCATGA | 3 cut(s) | 238, 469, 532 |
| CfoI | GCGC | 1 cut(s) | 1603 |
| Cfr10I | RCCGGY | 1 cut(s) | 287 |
| Cfr13I | GGNCC | 4 cut(s) | 179, 814, 1433, 1527 |
| ClaI | ATCGAT | 1 cut(s) | 444 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 984, 1019 |
| CviAII | CATG | 7 cut(s) | 239, 389, 470, 533, 776, 818, 911 |
| DdeI | CTNAG | 8 cut(s) | 103, 630, 690, 769, 1281, 1401, 1494, 1548 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 631 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 631 |
| Eam1104I | CTCTTC | 3 cut(s) | 797, 1263, 1552 |
| EarI | CTCTTC | 3 cut(s) | 797, 1263, 1552 |
| Eco130I | CCWWGG | 1 cut(s) | 817 |
| Eco147I | AGGCCT | 1 cut(s) | 1343 |
| Eco24I | GRGCYC | 1 cut(s) | 1587 |
| Eco47I | GGWCC | 3 cut(s) | 179, 814, 1433 |
| Eco57I | CTGAAG | 3 cut(s) | 906, 1083, 1524 |
| Eco88I | CYCGRG | 1 cut(s) | 1203 |
| EcoO109I | RGGNCCY | 1 cut(s) | 179 |
| EcoRII | CCWGG | 3 cut(s) | 565, 832, 1579 |
| EcoT14I | CCWWGG | 1 cut(s) | 817 |
| EcoT22I | ATGCAT | 1 cut(s) | 493 |
| EcoT38I | GRGCYC | 1 cut(s) | 1587 |
| ErhI | CCWWGG | 1 cut(s) | 817 |
| FaeI | CATG | 7 cut(s) | 242, 392, 473, 536, 779, 821, 914 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 180, 212, 576, 608, 1371, 1403 |
| FatI | CATG | 7 cut(s) | 238, 388, 469, 532, 775, 817, 910 |
| FauI | CCCGC | 1 cut(s) | 1518 |
| FbaI | TGATCA | 4 cut(s) | 232, 466, 529, 1216 |
| Fnu4HI | GCNGC | 9 cut(s) | 21, 69, 261, 678, 762, 895, 1419, 1491, 1599 |
| FokI | GGATG | 2 cut(s) | 128, 1226 |
| FriOI | GRGCYC | 1 cut(s) | 1587 |
| Fsp4HI | GCNGC | 9 cut(s) | 21, 69, 261, 678, 762, 895, 1419, 1491, 1599 |
| FspBI | CTAG | 3 cut(s) | 92, 327, 924 |
| FspI | TGCGCA | 1 cut(s) | 1602 |
| GlaI | GCGC | 1 cut(s) | 1602 |
| GluI | GCNGC | 9 cut(s) | 21, 69, 261, 678, 762, 895, 1419, 1491, 1599 |
| GsaI | CCCAGC | 1 cut(s) | 58 |
| GsuI | CTGGAG | 1 cut(s) | 180 |
| HaeIII | GGCC | 2 cut(s) | 1343, 1529 |
| HapII | CCGG | 2 cut(s) | 17, 288 |
| HhaI | GCGC | 1 cut(s) | 1603 |
| Hin1II | CATG | 7 cut(s) | 242, 392, 473, 536, 779, 821, 914 |
| Hin6I | GCGC | 1 cut(s) | 1601 |
| HinP1I | GCGC | 1 cut(s) | 1601 |
| HpaII | CCGG | 2 cut(s) | 17, 288 |
| HphI | GGTGA | 7 cut(s) | 302, 410, 419, 476, 554, 622, 1297 |
| Hpy166II | GTNNAC | 2 cut(s) | 61, 1006 |
| Hpy8I | GTNNAC | 2 cut(s) | 61, 1006 |
| HpyAV | CCTTC | 6 cut(s) | 48, 414, 578, 947, 1492, 1548 |
| HpyCH4III | ACNGT | 2 cut(s) | 140, 1450 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 77, 686, 1078 |
| HpyF3I | CTNAG | 8 cut(s) | 103, 630, 690, 769, 1281, 1401, 1494, 1548 |
| Hsp92II | CATG | 7 cut(s) | 242, 392, 473, 536, 779, 821, 914 |
| HspAI | GCGC | 1 cut(s) | 1601 |
| Ksp22I | TGATCA | 4 cut(s) | 232, 466, 529, 1216 |
| LmnI | GCTCC | 4 cut(s) | 902, 1296, 1396, 1402 |
| Lsp1109I | GCAGC | 8 cut(s) | 7, 55, 272, 689, 748, 1405, 1477, 1585 |
| LweI | GCATC | 8 cut(s) | 67, 274, 391, 500, 664, 676, 1036, 1048 |
| MaeI | CTAG | 3 cut(s) | 92, 327, 924 |
| MaeIII | GTNAC | 3 cut(s) | 872, 1285, 1438 |
| MfeI | CAATTG | 2 cut(s) | 216, 996 |
| MflI | RGATCY | 3 cut(s) | 88, 587, 1576 |
| MhlI | GDGCHC | 3 cut(s) | 1301, 1457, 1587 |
| MluCI | AATT | 9 cut(s) | 216, 249, 309, 331, 450, 546, 554, 996, 1588 |
| MlyI | GAGTC | 3 cut(s) | 164, 641, 1001 |
| Mph1103I | ATGCAT | 1 cut(s) | 493 |
| MroXI | GAANNNNTTC | 1 cut(s) | 603 |
| MseI | TTAA | 4 cut(s) | 369, 377, 1115, 1568 |
| MslI | CAYNNNNRTG | 2 cut(s) | 276, 393 |
| MspA1I | CMGCKG | 2 cut(s) | 230, 680 |
| MspI | CCGG | 2 cut(s) | 17, 288 |
| MspR9I | CCNGG | 4 cut(s) | 17, 567, 834, 1581 |
| MunI | CAATTG | 2 cut(s) | 216, 996 |
| Mva1269I | GAATGC | 1 cut(s) | 1081 |
| MvaI | CCWGG | 3 cut(s) | 567, 834, 1581 |
| MvnI | CGCG | 1 cut(s) | 1525 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 77, 686, 1078 |
| NciI | CCSGG | 1 cut(s) | 17 |
| NcoI | CCATGG | 1 cut(s) | 817 |
| NlaIII | CATG | 7 cut(s) | 242, 392, 473, 536, 779, 821, 914 |
| NlaIV | GGNNCC | 3 cut(s) | 655, 898, 1176 |
| NmuCI | GTSAC | 1 cut(s) | 1285 |
| NsbI | TGCGCA | 1 cut(s) | 1602 |
| NsiI | ATGCAT | 1 cut(s) | 493 |
| PagI | TCATGA | 3 cut(s) | 238, 469, 532 |
| PceI | AGGCCT | 1 cut(s) | 1343 |
| PctI | GAATGC | 1 cut(s) | 1081 |
| PdmI | GAANNNNTTC | 1 cut(s) | 603 |
| PfeI | GAWTC | 7 cut(s) | 338, 791, 857, 1141, 1459, 1465, 1472 |
| PkrI | GCNGC | 9 cut(s) | 22, 70, 262, 679, 763, 896, 1420, 1492, 1600 |
| PleI | GAGTC | 3 cut(s) | 163, 640, 1001 |
| PpsI | GAGTC | 3 cut(s) | 163, 640, 1001 |
| PpuMI | RGGWCCY | 1 cut(s) | 179 |
| Psp5II | RGGWCCY | 1 cut(s) | 179 |
| Psp6I | CCWGG | 3 cut(s) | 565, 832, 1579 |
| PspFI | CCCAGC | 1 cut(s) | 54 |
| PspGI | CCWGG | 3 cut(s) | 565, 832, 1579 |
| PspN4I | GGNNCC | 3 cut(s) | 655, 898, 1176 |
| PspPI | GGNCC | 4 cut(s) | 179, 814, 1433, 1527 |
| PspPPI | RGGWCCY | 1 cut(s) | 179 |
| PstI | CTGCAG | 2 cut(s) | 187, 1252 |
| PstNI | CAGNNNCTG | 1 cut(s) | 158 |
| PsuI | RGATCY | 3 cut(s) | 88, 587, 1576 |
| PvuII | CAGCTG | 2 cut(s) | 230, 680 |
| RseI | CAYNNNNRTG | 2 cut(s) | 276, 393 |
| SaqAI | TTAA | 4 cut(s) | 369, 377, 1115, 1568 |
| SatI | GCNGC | 9 cut(s) | 21, 69, 261, 678, 762, 895, 1419, 1491, 1599 |
| Sau96I | GGNCC | 4 cut(s) | 179, 814, 1433, 1527 |
| SchI | GAGTC | 3 cut(s) | 164, 641, 1001 |
| ScrFI | CCNGG | 4 cut(s) | 17, 567, 834, 1581 |
| SduI | GDGCHC | 3 cut(s) | 1301, 1457, 1587 |
| SfaNI | GCATC | 8 cut(s) | 67, 274, 391, 500, 664, 676, 1036, 1048 |
| SfcI | CTRYAG | 4 cut(s) | 166, 183, 210, 1248 |
| SinI | GGWCC | 3 cut(s) | 179, 814, 1433 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 276, 393 |
| SmlI | CTYRAG | 1 cut(s) | 1345 |
| SmoI | CTYRAG | 1 cut(s) | 1345 |
| Sse9I | AATT | 9 cut(s) | 216, 249, 309, 331, 450, 546, 554, 996, 1588 |
| SseBI | AGGCCT | 1 cut(s) | 1343 |
| SsiI | CCGC | 3 cut(s) | 894, 914, 1525 |
| SspI | AATATT | 2 cut(s) | 604, 787 |
| SspMI | CTAG | 3 cut(s) | 92, 327, 924 |
| StuI | AGGCCT | 1 cut(s) | 1343 |
| StyD4I | CCNGG | 4 cut(s) | 15, 565, 832, 1579 |
| StyI | CCWWGG | 1 cut(s) | 817 |
| TaaI | ACNGT | 2 cut(s) | 140, 1450 |
| TaqI | TCGA | 1 cut(s) | 444 |
| TasI | AATT | 9 cut(s) | 216, 249, 309, 331, 450, 546, 554, 996, 1588 |
| TauI | GCSGC | 1 cut(s) | 897 |
| TfiI | GAWTC | 7 cut(s) | 338, 791, 857, 1141, 1459, 1465, 1472 |
| Tru1I | TTAA | 4 cut(s) | 369, 377, 1115, 1568 |
| Tru9I | TTAA | 4 cut(s) | 369, 377, 1115, 1568 |
| TscAI | CASTG | 6 cut(s) | 79, 760, 1306, 1374, 1435, 1455 |
| TseFI | GTSAC | 1 cut(s) | 1285 |
| TseI | GCWGC | 8 cut(s) | 20, 68, 260, 677, 761, 1418, 1490, 1598 |
| Tsp45I | GTSAC | 1 cut(s) | 1285 |
| TspDTI | ATGAA | 5 cut(s) | 138, 255, 405, 588, 1083 |
| TspGWI | ACGGA | 2 cut(s) | 596, 1024 |
| TspRI | CASTG | 6 cut(s) | 79, 760, 1306, 1374, 1435, 1455 |
| VpaK11BI | GGWCC | 3 cut(s) | 179, 814, 1433 |
| XapI | RAATTY | 2 cut(s) | 249, 554 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 51 |
| XmnI | GAANNNNTTC | 1 cut(s) | 603 |
| XspI | CTAG | 3 cut(s) | 92, 327, 924 |
| Zsp2I | ATGCAT | 1 cut(s) | 493 |