Rh4BG173300

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001352
Physical Location & Seq
Reverse (-)
30923215 .. 30928849
5635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG173300.1

Sequence Viewer

Length: 2100 bp
ATGGATCACCAAGATCTCAGGCGAGCCCACAGTTCAGGGCACGAGAGTCACCATGTGTGTCACAAATGCGGGTGGCCTTTTCCGAATCCGCACCCGAGTGCTAAAAACAGACGCGCGCATAAGAAGATTTGCGGAACCGTTGAGGGTTACAAGGTGGCTGGCGCCGAGGGGAGTGCACAGTTGAGTGTCTCTGATGATGAGCAGCACTCTGATGAAGATCCCAAAACCCCCGGTCCGAAAGTCTTGGAAAGGAGCGTTTATGAGAAGGGTAGTGGCGTAATTGGGCAAGGATCTATTAAATCAGATGATGGGGTGTTCTCGGATGCTGTTCAGGAGTTTTCAGATAGCGGTTCTGCTGCATGGACCGGAGAAGGGTTAGAGGATGCCCCGGGATCAGCACCTAATGTAGAAAGGGGTGCCAAGAGTGATTTGAATGCCATTCCGTTCCACAATGATGGGGGAACGACAGATGCTGGTTACAATGAAAATGCTGAAACCTCTAACTTATGTGGGGATGACAAAAGCAGAGGAGTTGCAGAGAATTTTTCCACAGATCCTGCAAGTAATTTGGAATCTGTTAGTTGTCTATCTGAACTCCAAGTTAATCCAGTAACCAATCTACATGAAAGTGGTGATGCTGCTGGTGATTGTGAGAAGGATAAAACCGAAAAGTGTGATATAACTGGGTATGATGGTGATCATGAGAAAGCTGAAAAGTATGCATCATCTGGTGAAGAAATAAGTAAAAGATCTACAGAAAAAACCTTTGCGGAGGACTCTACCACTAATTTGGAATCCATCATTACTCTCTCCGACTCTCAAGTTCATCCATCGGGAAAGTTGCCTGATGTTGATGGCTCTGCTGATCGTGATGAGGGTGAAATTGAGAAATTTGTTATATCTGGAAATGAAAGCATGGAAGGATCAGTTGAAGAAAATATTCTGGTGAAGTCAGAATTGACCCCCGAGTCTTCTGCCAATCTAACTGGTTCAAAAGCTATTGTTGAAGATGCAGCCAATGGTTCTAGGATGGAGCTGGTTGAAACCAGTGGCAGAGATTTAGATAGCGGTTCGAATTCTAGCAAAGTTGCAGGAAAAATTCTATCAGATGCTCAGCAAGATGTGTTAGAAAATGAGATTGCTTGCAATGACAAAATCCTGAAAGAAGTTGATGCCGGTGTTGCTGCTGGCTCAGATCATGGTCAGAATGTTGAATCGTTCCAGAAGAGTTTGGAGGACCATGGGAAGAAAGAACCAGGGTTTTCTGCTTTAGATACCGAATCTTCTGTTCAAAGTTTCATTGCCTCTGAAGATAGCGGCTCCCAAGAGGTCCATGCGGTTGCTTCTGGGATTACTTCTCAATCTTTGTTAGAGGAAGGCGATAATAACCTTGTCAGACAACAGCCAAGCTCAACTGCAATTGATGTGGACTCAAATAGCCAAACTGACAGTCTGGAAGCCAACTGGGGATCTTTCTCAGTTCTTTCCATGCAATCTGATGCTGAAACTGTGCCACATACGCATTCCCAATCTTCATTAGGCGAAAACTCGGGCAAATCTGATATGTTTGAGGCACCATCTTTCATGACATTGGTTGAACCCCGAGGTGGGGATGATCAAAAAGCTACTGGTTCTGAAATCCTGACTGCAGTACAGAACCCAGAACAGCCAAAACCTGCTTCTTTGCAAACTGGGTGGTTTCCTTCTATGACTCATGTTGTGAATGAGTCACCGGGGAGAAAGAAGAACGAAGAGATCATTGCCAAGGTGACAAACAGGAGCACTGGGAAGCAACACACTCCTTTGAAAAACCTTTTGGGGGAGGCCTCAAGTGAAAGCAACGGAAAGCCACTGAACCAAAAAGAAAGTCTTGCTCCTGCTCCTCAGAAAGATGATAAAGCTGCAATGGTCAGCGGTCCTGTAACAACAACAGTGAGCTCGATTCTGAATCCTGAATCGCCGACTGATCAGGCTGCTAAGAAAGAGACTGCAAAAGAATGGAACTCGCCGGCCAGATATCCTTCAGACACTAAGAGTGAGAAGAGGAAAGTAAAGGGGAGATCCTGGGCACAATTTGTGTGCTGCGCTTCTGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

699

Amino Acids

74.25

Weight (kDa)

4.71

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 967
Acc36I ACCTGC 1 cut(s) 1684
AccB1I GGYRCC 3 cut(s) 161, 416, 1573
AccII CGCG 2 cut(s) 114, 116
AciI CCGC 9 cut(s) 69, 89, 132, 348, 770, 1068, 1317, 1337, 1914
AclWI GGATC 8 cut(s) 12, 212, 298, 400, 548, 931, 1477, 2055
AcoI YGGCCR 1 cut(s) 2010
AcsI RAATTY 4 cut(s) 541, 890, 1075, 1098
AcuI CTGAAG 2 cut(s) 1329, 2007
AcyI GRCGYC 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 1 cut(s) 1653
AfiI CCNNNNNNNGG 5 cut(s) 372, 1607, 1608, 1609, 1819
AgsI TTSAA 9 cut(s) 433, 932, 993, 1007, 1043, 1214, 1292, 1598, 1807
AjnI CCWGG 2 cut(s) 1255, 2063
AjuI GAANNNNNNNTTGG 4 cut(s) 1663, 1695, 1799, 1831
AleI CACNNNNGTG 1 cut(s) 96
AluBI AGCT 7 cut(s) 710, 998, 1036, 1410, 1625, 1901, 1938
AluI AGCT 7 cut(s) 710, 998, 1036, 1410, 1625, 1901, 1938
Alw21I GWGCWC 3 cut(s) 178, 1784, 1940
Alw26I GTCTC 2 cut(s) 193, 1979
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 8 cut(s) 12, 212, 298, 400, 548, 931, 1477, 2055
AlwNI CAGNNNCTG 2 cut(s) 473, 557
Ama87I CYCGRG 5 cut(s) 94, 388, 965, 1549, 1602
AoxI GGCC 3 cut(s) 74, 1824, 2010
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 8 cut(s) 202, 356, 638, 1013, 1184, 1901, 1973, 2082
ApoI RAATTY 4 cut(s) 541, 890, 1075, 1098
ArsI GACNNNNNNTTYG 4 cut(s) 1435, 1467, 1852, 1884
Asp700I GAANNNNTTC 1 cut(s) 939
AspLEI GCGC 4 cut(s) 116, 118, 164, 2087
AspS9I GGNCC 5 cut(s) 233, 363, 1237, 1330, 1916
AsuC2I CCSGG 4 cut(s) 231, 389, 390, 1734
AsuHPI GGTGA 9 cut(s) 41, 644, 656, 707, 743, 890, 958, 1722, 1780
AsuII TTCGAA 1 cut(s) 1073
AvaI CYCGRG 5 cut(s) 94, 388, 965, 1549, 1602
AvaII GGWCC 5 cut(s) 233, 363, 1237, 1330, 1916
BaeGI GKGCMC 3 cut(s) 42, 178, 2071
BanI GGYRCC 3 cut(s) 161, 416, 1573
BanII GRGCYC 2 cut(s) 28, 1940
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 963
Bbv12I GWGCWC 3 cut(s) 178, 1784, 1940
BbvI GCAGC 8 cut(s) 214, 343, 625, 1025, 1171, 1888, 1960, 2069
BccI CCATC 8 cut(s) 302, 449, 686, 806, 838, 848, 1024, 1585
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 956, 990
BciT130I CCWGG 2 cut(s) 1257, 2065
BclI TGATCA 3 cut(s) 697, 1615, 1966
BcnI CCSGG 4 cut(s) 231, 389, 390, 1734
BcoDI GTCTC 2 cut(s) 193, 1979
BfaI CTAG 2 cut(s) 1026, 1080
BfmI CTRYAG 2 cut(s) 753, 1647
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 1684
BglII AGATCT 2 cut(s) 13, 749
BisI GCNGC 9 cut(s) 203, 357, 639, 1014, 1185, 1318, 1902, 1974, 2083
BlpI GCTNAGC 1 cut(s) 1113
BlsI GCNGC 9 cut(s) 204, 358, 640, 1015, 1186, 1319, 1903, 1975, 2084
Bme1390I CCNGG 6 cut(s) 231, 389, 390, 1257, 1734, 2065
Bme18I GGWCC 5 cut(s) 233, 363, 1237, 1330, 1916
BmeT110I CYCGRG 5 cut(s) 94, 388, 965, 1549, 1602
BmgT120I GGNCC 5 cut(s) 233, 363, 1237, 1330, 1916
BmiI GGNNCC 5 cut(s) 136, 163, 418, 1321, 1575
BmrFI CCNGG 6 cut(s) 231, 389, 390, 1257, 1734, 2065
BmrI ACTGGG 4 cut(s) 693, 1474, 1701, 1794
BmsI GCATC 9 cut(s) 313, 373, 460, 625, 731, 1000, 1099, 1162, 1489
BmuI ACTGGG 4 cut(s) 693, 1474, 1701, 1794
BpiI GAAGAC 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 191, 223
Bpu1102I GCTNAGC 1 cut(s) 1113
Bpu14I TTCGAA 1 cut(s) 1073
BpuEI CTTGAG 2 cut(s) 804, 1813
BpuMI CCSGG 4 cut(s) 231, 389, 390, 1734
BsaBI GATNNNNATC 2 cut(s) 216, 696
BsaHI GRCGYC 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 1607, 1608, 1609, 1819
Bse118I RCCGGY 2 cut(s) 1175, 2008
Bse1I ACTGG 8 cut(s) 608, 688, 991, 1047, 1469, 1633, 1696, 1789
Bse3DI GCAATG 4 cut(s) 1153, 1299, 1758, 1911
Bse8I GATNNNNATC 2 cut(s) 216, 696
BseBI CCWGG 2 cut(s) 1257, 2065
BseGI GGATG 6 cut(s) 328, 388, 520, 826, 1035, 1618
BseJI GATNNNNATC 2 cut(s) 216, 696
BseLI CCNNNNNNNGG 5 cut(s) 372, 1607, 1608, 1609, 1819
BseMI GCAATG 4 cut(s) 1153, 1299, 1758, 1911
BseMII CTCAG 5 cut(s) 31, 1127, 1206, 1491, 1898
BseNI ACTGG 8 cut(s) 608, 688, 991, 1047, 1469, 1633, 1696, 1789
BsePI GCGCGC 1 cut(s) 114
BseRI GAGGAG 2 cut(s) 543, 1872
BseSI GKGCMC 3 cut(s) 42, 178, 2071
BseXI GCAGC 8 cut(s) 214, 343, 625, 1025, 1171, 1888, 1960, 2069
Bsh1236I CGCG 2 cut(s) 114, 116
BshFI GGCC 3 cut(s) 76, 1826, 2012
BshNI GGYRCC 3 cut(s) 161, 416, 1573
BsiHKAI GWGCWC 3 cut(s) 178, 1784, 1940
BsiHKCI CYCGRG 5 cut(s) 94, 388, 965, 1549, 1602
BsiSI CCGG 6 cut(s) 231, 366, 389, 1176, 1733, 2009
BslI CCNNNNNNNGG 5 cut(s) 372, 1607, 1608, 1609, 1819
BsmAI GTCTC 2 cut(s) 193, 1979
BsmI GAATGC 2 cut(s) 439, 1522
BsnI GGCC 3 cut(s) 76, 1826, 2012
BsoBI CYCGRG 5 cut(s) 94, 388, 965, 1549, 1602
Bsp119I TTCGAA 1 cut(s) 1073
Bsp1286I GDGCHC 6 cut(s) 28, 42, 178, 1784, 1940, 2071
Bsp1720I GCTNAGC 1 cut(s) 1113
Bsp19I CCATGG 1 cut(s) 1240
BspACI CCGC 9 cut(s) 69, 89, 132, 348, 770, 1068, 1317, 1337, 1914
BspANI GGCC 3 cut(s) 76, 1826, 2012
BspCNI CTCAG 5 cut(s) 30, 1126, 1205, 1490, 1897
BspFNI CGCG 2 cut(s) 114, 116
BspHI TCATGA 2 cut(s) 700, 1584
BspLI GGNNCC 5 cut(s) 136, 163, 418, 1321, 1575
BspMAI CTGCAG 1 cut(s) 1651
BspMI ACCTGC 1 cut(s) 1684
BspPI GGATC 8 cut(s) 12, 212, 298, 400, 548, 931, 1477, 2055
BspT104I TTCGAA 1 cut(s) 1073
BspT107I GGYRCC 3 cut(s) 161, 416, 1573
BsrDI GCAATG 4 cut(s) 1153, 1299, 1758, 1911
BsrFI RCCGGY 2 cut(s) 1175, 2008
BsrI ACTGG 8 cut(s) 608, 688, 991, 1047, 1469, 1633, 1696, 1789
BssAI RCCGGY 2 cut(s) 1175, 2008
BssHII GCGCGC 1 cut(s) 114
BssNI GRCGYC 1 cut(s) 162
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 2 cut(s) 1240, 1764
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 2 cut(s) 1257, 2065
Bst4CI ACNGT 6 cut(s) 32, 139, 180, 1451, 1510, 1933
Bst6I CTCTTC 3 cut(s) 1220, 1746, 2036
BstACI GRCGYC 1 cut(s) 162
BstBI TTCGAA 1 cut(s) 1073
BstC8I GCNNGC 6 cut(s) 24, 116, 160, 1144, 1189, 2010
BstDEI CTNAG 7 cut(s) 17, 1113, 1192, 1477, 1884, 1977, 2031
BstDSI CCRYGG 1 cut(s) 1240
BstF5I GGATG 6 cut(s) 328, 388, 520, 826, 1035, 1618
BstFNI CGCG 2 cut(s) 114, 116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 116, 118, 164, 2087
BstMAI GTCTC 2 cut(s) 193, 1979
BstMWI GCNNNNNNNGC 2 cut(s) 1181, 1519
BstNI CCWGG 2 cut(s) 1257, 2065
BstSCI CCNGG 6 cut(s) 229, 387, 388, 1255, 1732, 2063
BstSFI CTRYAG 2 cut(s) 753, 1647
BstSLI GKGCMC 3 cut(s) 42, 178, 2071
BstUI CGCG 2 cut(s) 114, 116
BstV1I GCAGC 8 cut(s) 214, 343, 625, 1025, 1171, 1888, 1960, 2069
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 7 cut(s) 13, 217, 290, 553, 749, 1469, 2060
BstXI CCANNNNNNTGG 2 cut(s) 455, 790
BstYI RGATCY 7 cut(s) 13, 217, 290, 553, 749, 1469, 2060
BsuRI GGCC 3 cut(s) 76, 1826, 2012
BtgI CCRYGG 1 cut(s) 1240
BtsCI GGATG 6 cut(s) 328, 388, 520, 826, 1035, 1618
BtsIMutI CAGTG 4 cut(s) 1054, 1782, 1850, 1938
BveI ACCTGC 1 cut(s) 1684
Cac8I GCNNGC 6 cut(s) 24, 116, 160, 1144, 1189, 2010
CaiI CAGNNNCTG 2 cut(s) 473, 557
CciI TCATGA 2 cut(s) 700, 1584
CfoI GCGC 4 cut(s) 116, 118, 164, 2087
Cfr10I RCCGGY 2 cut(s) 1175, 2008
Cfr13I GGNCC 5 cut(s) 233, 363, 1237, 1330, 1916
Cfr9I CCCGGG 1 cut(s) 388
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 1652
CspCI CAANNNNNGTGG 4 cut(s) 1407, 1442, 1676, 1711
CspI CGGWCCG 1 cut(s) 233
CviQI GTAC 1 cut(s) 1652
DdeI CTNAG 7 cut(s) 17, 1113, 1192, 1477, 1884, 1977, 2031
DinI GGCGCC 1 cut(s) 163
DraIII CACNNNGTG 1 cut(s) 55
DrdI GACNNNNNNGTC 1 cut(s) 967
DseDI GACNNNNNNGTC 1 cut(s) 967
EaeI YGGCCR 1 cut(s) 2010
Eam1104I CTCTTC 3 cut(s) 1220, 1746, 2036
EarI CTCTTC 3 cut(s) 1220, 1746, 2036
Ecl136II GAGCTC 1 cut(s) 1938
Eco130I CCWWGG 2 cut(s) 1240, 1764
Eco147I AGGCCT 1 cut(s) 1826
Eco24I GRGCYC 2 cut(s) 28, 1940
Eco32I GATATC 1 cut(s) 2018
Eco47I GGWCC 5 cut(s) 233, 363, 1237, 1330, 1916
Eco53kI GAGCTC 1 cut(s) 1938
Eco57I CTGAAG 2 cut(s) 1329, 2007
Eco88I CYCGRG 5 cut(s) 94, 388, 965, 1549, 1602
EcoICRI GAGCTC 1 cut(s) 1938
EcoRI GAATTC 1 cut(s) 1075
EcoRII CCWGG 2 cut(s) 1255, 2063
EcoRV GATATC 1 cut(s) 2018
EcoT14I CCWWGG 2 cut(s) 1240, 1764
EcoT22I ATGCAT 1 cut(s) 724
EcoT38I GRGCYC 2 cut(s) 28, 1940
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 1240, 1764
FalI AAGNNNNNCTT 2 cut(s) 1854, 1886
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 3 cut(s) 697, 1615, 1966
Fnu4HI GCNGC 9 cut(s) 203, 357, 639, 1014, 1185, 1318, 1902, 1974, 2083
FokI GGATG 6 cut(s) 335, 395, 527, 813, 1042, 1625
FriOI GRGCYC 2 cut(s) 28, 1940
Fsp4HI GCNGC 9 cut(s) 203, 357, 639, 1014, 1185, 1318, 1902, 1974, 2083
FspBI CTAG 2 cut(s) 1026, 1080
GlaI GCGC 4 cut(s) 115, 117, 163, 2086
GluI GCNGC 9 cut(s) 203, 357, 639, 1014, 1185, 1318, 1902, 1974, 2083
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 76, 1826, 2012
HapII CCGG 6 cut(s) 231, 366, 389, 1176, 1733, 2009
HgaI GACGC 1 cut(s) 120
HhaI GCGC 4 cut(s) 116, 118, 164, 2087
Hin1I GRCGYC 1 cut(s) 162
Hin6I GCGC 4 cut(s) 114, 116, 162, 2085
HinP1I GCGC 4 cut(s) 114, 116, 162, 2085
HpaII CCGG 6 cut(s) 231, 366, 389, 1176, 1733, 2009
HphI GGTGA 9 cut(s) 41, 644, 656, 707, 743, 890, 958, 1722, 1780
Hpy166II GTNNAC 2 cut(s) 176, 1429
Hpy8I GTNNAC 2 cut(s) 176, 1429
HpyAV CCTTC 7 cut(s) 259, 365, 649, 914, 1370, 1713, 2031
HpyCH4III ACNGT 6 cut(s) 32, 139, 180, 1451, 1510, 1933
HpyF10VI GCNNNNNNNGC 2 cut(s) 1181, 1519
HpyF3I CTNAG 7 cut(s) 17, 1113, 1192, 1477, 1884, 1977, 2031
Hsp92I GRCGYC 1 cut(s) 162
HspAI GCGC 4 cut(s) 114, 116, 162, 2085
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 2008
KroNI GCCGGC 1 cut(s) 2010
Ksp22I TGATCA 3 cut(s) 697, 1615, 1966
LmnI GCTCC 6 cut(s) 252, 1033, 1325, 1779, 1879, 1885
Lsp1109I GCAGC 8 cut(s) 214, 343, 625, 1025, 1171, 1888, 1960, 2069
LweI GCATC 9 cut(s) 313, 373, 460, 625, 731, 1000, 1099, 1162, 1489
MaeI CTAG 2 cut(s) 1026, 1080
MaeIII GTNAC 8 cut(s) 47, 59, 146, 476, 610, 1728, 1768, 1921
MfeI CAATTG 1 cut(s) 1419
MflI RGATCY 7 cut(s) 13, 217, 290, 553, 749, 1469, 2060
MhlI GDGCHC 6 cut(s) 28, 42, 178, 1784, 1940, 2071
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 7 cut(s) 55, 770, 809, 977, 1424, 1705, 1736
MmeI TCCRAC 1 cut(s) 837
Mph1103I ATGCAT 1 cut(s) 724
MroNI GCCGGC 1 cut(s) 2008
MroXI GAANNNNTTC 1 cut(s) 939
MseI TTAA 2 cut(s) 297, 603
MslI CAYNNNNRTG 4 cut(s) 96, 210, 453, 627
MspA1I CMGCKG 1 cut(s) 1914
MspI CCGG 6 cut(s) 231, 366, 389, 1176, 1733, 2009
MspR9I CCNGG 6 cut(s) 231, 389, 390, 1257, 1734, 2065
MunI CAATTG 1 cut(s) 1419
Mva1269I GAATGC 2 cut(s) 439, 1522
MvaI CCWGG 2 cut(s) 1257, 2065
MvnI CGCG 2 cut(s) 114, 116
MwoI GCNNNNNNNGC 2 cut(s) 1181, 1519
NaeI GCCGGC 1 cut(s) 2010
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 4 cut(s) 231, 389, 390, 1734
NcoI CCATGG 1 cut(s) 1240
NgoMIV GCCGGC 1 cut(s) 2008
NlaIV GGNNCC 5 cut(s) 136, 163, 418, 1321, 1575
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 4 cut(s) 47, 59, 1728, 1768
NsiI ATGCAT 1 cut(s) 724
NspV TTCGAA 1 cut(s) 1073
OliI CACNNNNGTG 1 cut(s) 96
PagI TCATGA 2 cut(s) 700, 1584
PauI GCGCGC 1 cut(s) 114
PceI AGGCCT 1 cut(s) 1826
PctI GAATGC 2 cut(s) 439, 1522
PdiI GCCGGC 1 cut(s) 2010
PdmI GAANNNNTTC 1 cut(s) 939
PfeI GAWTC 8 cut(s) 85, 572, 794, 1214, 1280, 1942, 1948, 1955
PkrI GCNGC 9 cut(s) 204, 358, 640, 1015, 1186, 1319, 1903, 1975, 2084
PleI GAGTC 7 cut(s) 54, 770, 809, 976, 1424, 1705, 1735
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 7 cut(s) 54, 770, 809, 976, 1424, 1705, 1735
Psp124BI GAGCTC 1 cut(s) 1940
Psp6I CCWGG 2 cut(s) 1255, 2063
PspGI CCWGG 2 cut(s) 1255, 2063
PspN4I GGNNCC 5 cut(s) 136, 163, 418, 1321, 1575
PspPI GGNCC 5 cut(s) 233, 363, 1237, 1330, 1916
PstI CTGCAG 1 cut(s) 1651
PstNI CAGNNNCTG 2 cut(s) 473, 557
PsuI RGATCY 7 cut(s) 13, 217, 290, 553, 749, 1469, 2060
PteI GCGCGC 1 cut(s) 114
RsaI GTAC 1 cut(s) 1653
RsaNI GTAC 1 cut(s) 1652
RseI CAYNNNNRTG 4 cut(s) 96, 210, 453, 627
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 1940
SaqAI TTAA 2 cut(s) 297, 603
SatI GCNGC 9 cut(s) 203, 357, 639, 1014, 1185, 1318, 1902, 1974, 2083
Sau96I GGNCC 5 cut(s) 233, 363, 1237, 1330, 1916
SchI GAGTC 7 cut(s) 55, 770, 809, 977, 1424, 1705, 1736
ScrFI CCNGG 6 cut(s) 231, 389, 390, 1257, 1734, 2065
SduI GDGCHC 6 cut(s) 28, 42, 178, 1784, 1940, 2071
SfaNI GCATC 9 cut(s) 313, 373, 460, 625, 731, 1000, 1099, 1162, 1489
SfcI CTRYAG 2 cut(s) 753, 1647
SfoI GGCGCC 1 cut(s) 163
SfuI TTCGAA 1 cut(s) 1073
SinI GGWCC 5 cut(s) 233, 363, 1237, 1330, 1916
SmaI CCCGGG 1 cut(s) 390
SmiMI CAYNNNNRTG 4 cut(s) 96, 210, 453, 627
SmlI CTYRAG 2 cut(s) 819, 1828
SmoI CTYRAG 2 cut(s) 819, 1828
SseBI AGGCCT 1 cut(s) 1826
SsiI CCGC 9 cut(s) 69, 89, 132, 348, 770, 1068, 1317, 1337, 1914
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 1 cut(s) 940
SspMI CTAG 2 cut(s) 1026, 1080
SstI GAGCTC 1 cut(s) 1940
StuI AGGCCT 1 cut(s) 1826
StyD4I CCNGG 6 cut(s) 229, 387, 388, 1255, 1732, 2063
StyI CCWWGG 2 cut(s) 1240, 1764
TaaI ACNGT 6 cut(s) 32, 139, 180, 1451, 1510, 1933
TaqI TCGA 2 cut(s) 1073, 1940
TatI WGTACW 1 cut(s) 1651
TauI GCSGC 1 cut(s) 1320
TfiI GAWTC 8 cut(s) 85, 572, 794, 1214, 1280, 1942, 1948, 1955
Tru1I TTAA 2 cut(s) 297, 603
Tru9I TTAA 2 cut(s) 297, 603
TscAI CASTG 4 cut(s) 1054, 1789, 1857, 1938
TseFI GTSAC 4 cut(s) 47, 59, 1728, 1768
TseI GCWGC 8 cut(s) 202, 356, 638, 1013, 1184, 1901, 1973, 2082
Tsp45I GTSAC 4 cut(s) 47, 59, 1728, 1768
TspDTI ATGAA 8 cut(s) 228, 498, 639, 815, 924, 1288, 1524, 1573
TspGWI ACGGA 2 cut(s) 432, 1857
TspMI CCCGGG 1 cut(s) 388
TspRI CASTG 4 cut(s) 1054, 1789, 1857, 1938
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 5 cut(s) 233, 363, 1237, 1330, 1916
XapI RAATTY 4 cut(s) 541, 890, 1075, 1098
XmaI CCCGGG 1 cut(s) 388
XmnI GAANNNNTTC 1 cut(s) 939
XspI CTAG 2 cut(s) 1026, 1080
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.