Rroxscaffold_2G00118800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001352
Physical Location & Seq
Forward (+)
50666940 .. 50668118
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118800.1

Sequence Viewer

Length: 546 bp
ATGGAGGGCAAACACCTGTTTTTGAAGCTAGCGAGTACGGGGTGCGGGTCAATCTTAACTATTCGATCGGCAAAAGGTGAGGAAATTACCCACCTCTTCATCAAAATATTTCCCGATGAGTCGGTACAGAAATCGATAATCTTTGCCCTCACCAGGGTTACGAGAAACCGCCTTAGACCTAAGGTGATAGACGAGGAGCTTATTCGAAAATATATTGAAAACACAAAGAAGCCGGCGGTGAAGGAGGATGAATTTGAGATGATGTCGGGGGATGAGGAGGAGGTGGAGAAGGAGACGCTGTTGGATTTGGGGGAAGAAGAACCGACAGAAGAAGGCAGGAAGCCCAATGTTCGGACTTTATCCACAAGGATCTGCAAGACGTGGCTGCATCGAATATTCATGTACCTATTACTAAGATATATCGATATGTATTGCCTTCGCGACCTGAACGACGTGGGGATGTTGCTCGACGTTATTGAGGCTTTCAAGTCAAGTCGAGCAAATCTAGAAAATAAGGTGTGGCTGGGTAGATCTTTAATCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

21.09

Weight (kDa)

6.02

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 441
AciI CCGC 3 cut(s) 45, 169, 236
AclWI GGATC 1 cut(s) 377
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 3 cut(s) 37, 126, 404
AfiI CCNNNNNNNGG 3 cut(s) 153, 154, 351
AgsI TTSAA 3 cut(s) 25, 218, 487
AjiI CACGTC 2 cut(s) 381, 454
AjnI CCWGG 1 cut(s) 152
AluBI AGCT 2 cut(s) 28, 199
AluI AGCT 2 cut(s) 28, 199
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 1 cut(s) 377
ApeKI GCWGC 1 cut(s) 385
ApoI RAATTY 1 cut(s) 251
AsuHPI GGTGA 4 cut(s) 89, 142, 196, 250
AsuII TTCGAA 1 cut(s) 205
AsuNHI GCTAGC 1 cut(s) 28
AxyI CCTNAGG 1 cut(s) 180
BbvI GCAGC 1 cut(s) 372
BciT130I CCWGG 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 2 cut(s) 29, 506
BglII AGATCT 1 cut(s) 530
BisI GCNGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 387
Bme1390I CCNGG 1 cut(s) 154
BmgBI CACGTC 2 cut(s) 381, 454
BmrFI CCNGG 1 cut(s) 154
BmsI GCATC 1 cut(s) 397
BmtI GCTAGC 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 205
Bsa29I ATCGAT 2 cut(s) 134, 423
BsaJI CCNNGG 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 154, 351
Bse118I RCCGGY 1 cut(s) 232
Bse21I CCTNAGG 1 cut(s) 180
BseBI CCWGG 1 cut(s) 154
BseCI ATCGAT 2 cut(s) 134, 423
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 3 cut(s) 253, 277, 465
BseLI CCNNNNNNNGG 3 cut(s) 153, 154, 351
BseRI GAGGAG 3 cut(s) 209, 290, 293
BseXI GCAGC 1 cut(s) 372
BseYI CCCAGC 1 cut(s) 523
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 68
BshVI ATCGAT 2 cut(s) 134, 423
BsiEI CGRYCG 1 cut(s) 68
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 3 cut(s) 153, 154, 351
BsmAI GTCTC 1 cut(s) 287
BsmBI CGTCTC 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 205
Bsp143I GATC 3 cut(s) 65, 369, 530
Bsp68I TCGCGA 1 cut(s) 441
BspACI CCGC 3 cut(s) 45, 169, 236
BspDI ATCGAT 2 cut(s) 134, 423
BspFNI CGCG 1 cut(s) 441
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 377
BspT104I TTCGAA 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 3 cut(s) 65, 369, 530
Bst2UI CCWGG 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 205
BstC8I GCNNGC 2 cut(s) 30, 234
BstDEI CTNAG 3 cut(s) 173, 180, 413
BstF5I GGATG 3 cut(s) 253, 277, 465
BstFNI CGCG 1 cut(s) 441
BstKTI GATC 3 cut(s) 68, 372, 533
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 3 cut(s) 65, 369, 530
BstMCI CGRYCG 1 cut(s) 68
BstNI CCWGG 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 152
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 369, 530
BstYI RGATCY 2 cut(s) 369, 530
Bsu15I ATCGAT 2 cut(s) 134, 423
Bsu36I CCTNAGG 1 cut(s) 180
BsuTUI ATCGAT 2 cut(s) 134, 423
BtrI CACGTC 2 cut(s) 381, 454
BtsCI GGATG 3 cut(s) 253, 277, 465
BtuMI TCGCGA 1 cut(s) 441
Cac8I GCNNGC 2 cut(s) 30, 234
Cfr10I RCCGGY 1 cut(s) 232
ClaI ATCGAT 2 cut(s) 134, 423
CseI GACGC 1 cut(s) 304
Csp6I GTAC 3 cut(s) 36, 125, 403
CviAII CATG 1 cut(s) 400
CviJI RGCY 7 cut(s) 28, 199, 232, 343, 385, 482, 523
CviKI_1 RGCY 7 cut(s) 28, 199, 232, 343, 385, 482, 523
CviQI GTAC 3 cut(s) 36, 125, 403
DdeI CTNAG 3 cut(s) 173, 180, 413
DpnI GATC 3 cut(s) 67, 371, 532
DpnII GATC 3 cut(s) 65, 369, 530
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco81I CCTNAGG 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 152
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 1 cut(s) 403
FaiI YATR 4 cut(s) 213, 401, 420, 428
FatI CATG 1 cut(s) 399
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 1 cut(s) 386
FokI GGATG 3 cut(s) 260, 284, 472
Fsp4HI GCNGC 1 cut(s) 386
FspBI CTAG 2 cut(s) 29, 506
GluI GCNGC 1 cut(s) 386
GsaI CCCAGC 1 cut(s) 527
HapII CCGG 1 cut(s) 233
HgaI GACGC 1 cut(s) 304
Hin1II CATG 1 cut(s) 403
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 233
HphI GGTGA 4 cut(s) 89, 142, 196, 250
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 3 cut(s) 113, 440, 506
Hpy99I CGWCG 2 cut(s) 455, 473
HpyAV CCTTC 4 cut(s) 235, 283, 326, 446
HpyCH4IV ACGT 3 cut(s) 380, 453, 471
HpyCH4V TGCA 2 cut(s) 375, 388
HpyF3I CTNAG 3 cut(s) 173, 180, 413
HpySE526I ACGT 3 cut(s) 380, 453, 471
Hsp92II CATG 1 cut(s) 403
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 3 cut(s) 65, 369, 530
LmnI GCTCC 1 cut(s) 196
LpnPI CCDG 7 cut(s) 29, 139, 166, 246, 322, 458, 509
Lsp1109I GCAGC 1 cut(s) 372
LweI GCATC 1 cut(s) 397
MaeI CTAG 2 cut(s) 29, 506
MaeII ACGT 3 cut(s) 380, 453, 471
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 67, 371, 532
MboI GATC 3 cut(s) 65, 369, 530
MboII GAAGA 4 cut(s) 88, 326, 329, 341
MflI RGATCY 2 cut(s) 369, 530
MluCI AATT 2 cut(s) 84, 251
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 9 cut(s) 73, 104, 158, 187, 238, 268, 271, 274, 472
MroNI GCCGGC 1 cut(s) 232
MseI TTAA 2 cut(s) 56, 536
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 154
MvaI CCWGG 1 cut(s) 154
MvnI CGCG 1 cut(s) 441
NaeI GCCGGC 1 cut(s) 234
NdeII GATC 3 cut(s) 65, 369, 530
NgoMIV GCCGGC 1 cut(s) 232
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 1 cut(s) 403
NruI TCGCGA 1 cut(s) 441
NspV TTCGAA 1 cut(s) 205
PdiI GCCGGC 1 cut(s) 234
PkrI GCNGC 1 cut(s) 387
Ple19I CGATCG 1 cut(s) 68
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 152
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 1 cut(s) 152
PsuI RGATCY 2 cut(s) 369, 530
PvuI CGATCG 1 cut(s) 68
RruI TCGCGA 1 cut(s) 441
RsaI GTAC 3 cut(s) 37, 126, 404
RsaNI GTAC 3 cut(s) 36, 125, 403
SaqAI TTAA 2 cut(s) 56, 536
SatI GCNGC 1 cut(s) 386
Sau3AI GATC 3 cut(s) 65, 369, 530
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 154
SfaNI GCATC 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 205
Sse9I AATT 2 cut(s) 84, 251
SsiI CCGC 3 cut(s) 45, 169, 236
SspI AATATT 2 cut(s) 108, 396
SspMI CTAG 2 cut(s) 29, 506
StyD4I CCNGG 1 cut(s) 152
TaiI ACGT 3 cut(s) 383, 456, 474
TaqI TCGA 7 cut(s) 64, 134, 205, 391, 423, 468, 496
TasI AATT 2 cut(s) 84, 251
Tru1I TTAA 2 cut(s) 56, 536
Tru9I TTAA 2 cut(s) 56, 536
TseI GCWGC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 88, 264, 388
XapI RAATTY 1 cut(s) 251
XbaI TCTAGA 1 cut(s) 505
XspI CTAG 2 cut(s) 29, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.