Rw4G014580

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001352
Physical Location & Seq
Reverse (-)
33727223 .. 33732849
5627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014580.1

Sequence Viewer

Length: 3666 bp
ATGGATCACCAAGATCTCAGGCGAGCCCACAGTTCAGGGCACGAGAGTCACCATGTGTGTCACAAATGCGGGTGGCCTTTTCCGAATCCGCACCCGAGTGCTAGACACAGACGCGCGCATAAGAAGATTTGCGGAACCGTTGAGGGTTACAAGGTGGCTGGCGCCGAGGGGAGTGCACAGTTGAGTGTCTCTGATGACGAGCAGCACTCTGATGAAGATCCCAAAACCCCCGGTCCGAAAGTCTTGGAAAGGAGCGTTTATGAGAGGGGTAGTGGCGGAATTGGGCAAGGATCTATTAAATCAGATGATGGGGTGTTCTCGGATGCTGTTCAGGAGTTTTCAGATAGCGGTTCTGCTGCATGGACCGGAGAAGGGTTGGAGGATGCCCCGGGATCAGCACCTAATGTAGAAAGGGGTGCCAAGAGTGATTTGAATGCCATTCCGTTCCACAATGATGGGGGAACGAAAGAAGACAAATCAAACCCCAATAAATATGAAATGGTGACAGATGCATCAGAAGATATGAAGAAGACCGATGTGACTGACAGCATGATTGAGAGCTGCTTAACATCTGTTGACCAGGAAGCTGAAGTGATAGGAATTACAGCAACTAATCTGGATAGGAACATCAGTTCAGGGACTACTTCTATTTCAGATGGTAGAACTGAAGAATCAAATGTGCTTCTTGGTGATAGCAATGGTTTACACCCCATTAAATATGAAATGGTGACAGATGCATCAGAAGCTATTAAAAAGACTGGTGCCACTGACAGCATGATTGAGAGCTGCTTAACATCTGTTGATCAGGATGCTGAAGTGACGGGAATTACAGCAACTAATCTAGATAGGAACATGATCTTACCCAATAAAGTTGCTGGTGAAACAGTTGAGGGTGGCTCTAAGTTAGAAGGATCAAATGAAATGACTTCAGAATATGTGCGAGATGATGAACTTGTTCCATCAGAGAAGGAGCATACTGATGGGTTTGATATGCATATTCCACAAATTGATGTCCCCCCTGAAGTAGAATCTGTTGCACATGTGAATACTTTTGTTGATACTGCTGAAAAGAAGGTGGATAATACAGATGGAATACAGTATCTCAGTCCTGATAAAGTGATAGAGAGTCGTAATGGGAAGGAGGAAGAGAATGCAAATGCAAATGTACATGTGCTTTCTGTGCCTGATGACATACATGTGGTAGATGATCCTGTGCTGATGCTTGAAGATTTCAGAAACCATAAAGTAAAGCAATTAGACCAAGTTACTTTCTTGGACTCATTGGAACAGTTTGATGATAAGGAAAATGATGTCAAACAACCTGTTTTCAACAAGAGTCTGAACATTTTCCAGTCAAGTCATCTAAGTGGGGGCACTGTTGTCTCTTCTGATATGCATGATTTGGAAGCTAGCCTTGAACCGGATCTCGGCAGCAGCAAGCCAATGATTAAAAGTGTGCCTGTTGAAGATGAAGCCAATATGTCTGAGACTGAGATCAAGATGTTTAAAAACCAAAGTTCAGATGAAATAGGAGCCCTGCAGATGCTAAAAGTAACTGCAGAAGAAAATCTTGTGGCAAATCCTATGGCTACTGTTCAATCTGCTGAAGGACAGGAATCTTATGAGAAGTCCAATGAGTCCGGTCAGGAATCTCATAATCTTATGAGAAACTGTGATGGGAGTGGAGAGTCTGGAGACCACAGCAGAAAAGTAACTGCAGAAGAAAATCTTGTGGCAAATCCTATAGCTAATGTTCAATCTGCTGAAGGACAGGTGTCTTATGAGAAGTCCAATGAGTCTTTACAGGTGAACTTTGCTGCTGGTGATGCTGGTGTAGATCAAACCACCCACTCGGTTGGTCCGGACGATAATGAAAAAGATGGAAGCTGTGATGGGAGTGGAGAATCTGGAGACTACAGCAGAAAAGGACCCGCAGAAGTAAATCTTGTGGCAACTCCTATAGCTACTGTTCAATCAGCTGATCATTATGAAAACCAAATTTATCCAGCAGCTAACTTACACAAAGTCGATGATGCCAGTGAGTATGAGAAGGGCGTAATTGAAAAGTGTAATAAAATTGGAGATGCTGGTTACAATGAAAACGCTGAAACCGCTAACTTATGTGGAGATGACAAAAGCAGAGGAGTTGCAGAGAATTTTTCCACAGATCCTGCAAGTAATTTGGAATCTGTTAGTTGTCTATCTGAAATCCAAGTTAATCCAGTAACCAATCTACATGAAAGTGGTGATGCTGCTGGTGATTGTGAGAAGGATAAAACCGAGAAGTGTGATATAACTGGGTATGATGGTGATCATGAGAAAGCTGAAAACTATGCATCATCTGGTGAAGAAATAAGTAAAAGATCTACAGAAAAAACCTTTGCGGAGGACTCTACCACTAATTTGGAATCCATCATTACTCTCTCCGACTCTCAAGTTCATCCATCGGGAAAGTTGCCTGATGTTGATGGCTCTGCTGATCGTGAAGAGGGTGAAATTGAGAAATTTGTTATATCTGGAAATGAAAGCATGGAAGGATCAGTTGAAGAAAATATTCTGGTGAAGTCAGAATTGACCCCCGAGTCTTCTGCCAATCTAACTGGTTCAAAAGCTATTGTTGAAGATGCAGCCAATGGTTCTAGGATGGAGCTGGTTGAAACCAGTGGCAGAGATTTAGATAGCGGTTCGAATTCTAGCAAAGTTGCAGGAAAAATTCTATCAGATGCTCAGCAAGATGTGTTAGAAAATGAGATTGCTTGCAATGACAAAATCCTGAAAGAAGTTGTTGCCGGTGTTGCTGCTGGGTCAGATCATGGTCAGAATGTTGAATCGTTCCAGAAGAGTTCGGAGGACCATGGGAAGAAAGAACCAGGGTTTTCTGCTTTAGATACCGAATCTTCTGTTCAAAGTTTCATTGCCTCTGAAGATAGCGGCTCCCAAGAGGTCCAGGCGGTTGCTTCTGGGATTACTTCTCAATCTTTGTTAGAGGAAGGCGATAATAACCTTGTCAGACATCAGCCAAGCTCAACTGCAATTGATGTGGACTCAAATAGCCAACTGACAGTCTGGAAGCCAACTGGGGATCTTTCTCAGGTATATATATTTGTCTCCTCAATGAACTTTTTTCTTTCCATGCAATCTGATGCTGAAACTGTGCCACATACGCATTCCCAATCTTCATTAGGTGAAAACTCGGGCAAATCTGATATGTTTGAGGCACCATCTTTCATGACATTGGTTGAACCCCGAGGTGGGGATGATCAAAAAGCTACTGGTTCTGAAATCCTGACTGCAGTACAGAACCCAGAACAGCCAAAACCTGCATCTTTGCAAACTGGGTGGTTTCCTTCTATGACTCATGTTGTGAATGAGTCACCGGGGAGAAAGAAGAACGAAGAGATCATTGCCAAGGTGACAAACAGGAGCACTGGGAAGCAACACACTCCTTTGAAAAACCTTTTGGGGGAGGCCTCAAGTGAAAGCAACGGAAAGCCACTGAACCAAAAAGAAAGTCTTGCTCTCGCTCCTCAGAAAGATGATAAAGCTGCAATGACTGCAAAAGAATGGAACTCGCCGGCCAGATATCCTTCAGACACTAAGAGTGAGAAGAGGAAGGTAAAGGGGAGATCCTGGGCACAATTTGTGTGCTGCGCTTCTGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1221

Amino Acids

131.21

Weight (kDa)

4.53

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2581
Acc36I ACCTGC 1 cut(s) 3328
AccB1I GGYRCC 4 cut(s) 161, 416, 761, 3217
AccII CGCG 2 cut(s) 114, 116
AccIII TCCGGA 1 cut(s) 1861
AcoI YGGCCR 1 cut(s) 3576
AcsI RAATTY 5 cut(s) 1998, 2155, 2504, 2689, 2712
AcuI CTGAAG 9 cut(s) 609, 687, 834, 912, 1041, 1626, 1785, 2943, 3573
AcyI GRCGYC 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 2 cut(s) 1167, 3297
AfiI CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3251, 3252, 3253, 3463
AflIII ACRYGT 3 cut(s) 1039, 1168, 1195
AjnI CCWGG 4 cut(s) 579, 2869, 2946, 3629
AjuI GAANNNNNNNTTGG 2 cut(s) 3443, 3475
AleI CACNNNNGTG 1 cut(s) 96
AloI GAACNNNNNNTCC 2 cut(s) 616, 648
Alw21I GWGCWC 2 cut(s) 178, 3428
Alw26I GTCTC 6 cut(s) 193, 1387, 1481, 1689, 1905, 3112
Alw44I GTGCAC 1 cut(s) 174
AlwNI CAGNNNCTG 1 cut(s) 2171
Ama87I CYCGRG 5 cut(s) 94, 388, 2579, 3193, 3246
Aor13HI TCCGGA 1 cut(s) 1861
AoxI GGCC 3 cut(s) 74, 3468, 3576
ApaLI GTGCAC 1 cut(s) 174
ApoI RAATTY 5 cut(s) 1998, 2155, 2504, 2689, 2712
ArsI GACNNNNNNTTYG 2 cut(s) 3496, 3528
Asp700I GAANNNNTTC 3 cut(s) 954, 1346, 2553
AspLEI GCGC 4 cut(s) 116, 118, 164, 3653
AspS9I GGNCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
AsuC2I CCSGG 4 cut(s) 231, 389, 390, 3378
AsuII TTCGAA 1 cut(s) 2687
AsuNHI GCTAGC 1 cut(s) 1409
AvaI CYCGRG 5 cut(s) 94, 388, 2579, 3193, 3246
AvaII GGWCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
BaeGI GKGCMC 4 cut(s) 42, 178, 1376, 3637
BanI GGYRCC 4 cut(s) 161, 416, 761, 3217
BanII GRGCYC 2 cut(s) 28, 1537
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 3 cut(s) 477, 536, 2577
Bbv12I GWGCWC 2 cut(s) 178, 3428
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 2570, 2604
BciT130I CCWGG 4 cut(s) 581, 2871, 2948, 3631
BclI TGATCA 4 cut(s) 802, 1981, 2311, 3259
BcnI CCSGG 4 cut(s) 231, 389, 390, 3378
BcoDI GTCTC 6 cut(s) 193, 1387, 1481, 1689, 1905, 3112
BfaI CTAG 5 cut(s) 102, 842, 1410, 2640, 2694
BfmI CTRYAG 8 cut(s) 1538, 1557, 1716, 1743, 1915, 1959, 2367, 3291
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 3328
BglII AGATCT 2 cut(s) 13, 2363
BlpI GCTNAGC 1 cut(s) 2727
Bme1390I CCNGG 8 cut(s) 231, 389, 390, 581, 2871, 2948, 3378, 3631
Bme18I GGWCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
BmeT110I CYCGRG 5 cut(s) 94, 388, 2579, 3193, 3246
BmgT120I GGNCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
BmiI GGNNCC 8 cut(s) 136, 163, 418, 763, 1534, 1930, 2935, 3219
BmrFI CCNGG 8 cut(s) 231, 389, 390, 581, 2871, 2948, 3378, 3631
BmrI ACTGGG 4 cut(s) 2307, 3087, 3345, 3438
BmtI GCTAGC 1 cut(s) 1413
BmuI ACTGGG 4 cut(s) 2307, 3087, 3345, 3438
BoxI GACNNNNGTC 1 cut(s) 1774
BpiI GAAGAC 3 cut(s) 477, 536, 2577
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 881, 913
BpmI CTGGAG 2 cut(s) 1713, 1929
Bpu1102I GCTNAGC 1 cut(s) 2727
Bpu14I TTCGAA 1 cut(s) 2687
BpuEI CTTGAG 2 cut(s) 2418, 3457
BpuMI CCSGG 4 cut(s) 231, 389, 390, 3378
BsaBI GATNNNNATC 2 cut(s) 216, 2310
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 1689
BsaWI WCCGGW 4 cut(s) 365, 1420, 1640, 1861
Bsc4I CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3251, 3252, 3253, 3463
Bse118I RCCGGY 2 cut(s) 2789, 3574
Bse3DI GCAATG 5 cut(s) 703, 2767, 2913, 3402, 3555
Bse8I GATNNNNATC 2 cut(s) 216, 2310
BseAI TCCGGA 1 cut(s) 1861
BseBI CCWGG 4 cut(s) 581, 2871, 2948, 3631
BseGI GGATG 6 cut(s) 328, 388, 814, 2440, 2649, 3262
BseJI GATNNNNATC 2 cut(s) 216, 2310
BseLI CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3251, 3252, 3253, 3463
BseMI GCAATG 5 cut(s) 703, 2767, 2913, 3402, 3555
BseMII CTCAG 7 cut(s) 31, 1117, 1476, 1482, 2741, 3104, 3542
BsePI GCGCGC 1 cut(s) 114
BseRI GAGGAG 3 cut(s) 2157, 3100, 3516
BseSI GKGCMC 4 cut(s) 42, 178, 1376, 3637
BseYI CCCAGC 1 cut(s) 2801
Bsh1236I CGCG 2 cut(s) 114, 116
BshFI GGCC 3 cut(s) 76, 3470, 3578
BshNI GGYRCC 4 cut(s) 161, 416, 761, 3217
BsiHKAI GWGCWC 2 cut(s) 178, 3428
BsiHKCI CYCGRG 5 cut(s) 94, 388, 2579, 3193, 3246
BsiSI CCGG 9 cut(s) 231, 366, 389, 1421, 1641, 1862, 2790, 3377, 3575
BslFI GGGAC 2 cut(s) 652, 998
BslI CCNNNNNNNGG 7 cut(s) 372, 1420, 1427, 3251, 3252, 3253, 3463
BsmAI GTCTC 6 cut(s) 193, 1387, 1481, 1689, 1905, 3112
BsmFI GGGAC 2 cut(s) 652, 998
BsmI GAATGC 3 cut(s) 439, 1156, 3166
BsnI GGCC 3 cut(s) 76, 3470, 3578
Bso31I GGTCTC 1 cut(s) 1689
BsoBI CYCGRG 5 cut(s) 94, 388, 2579, 3193, 3246
Bsp119I TTCGAA 1 cut(s) 2687
Bsp1286I GDGCHC 7 cut(s) 28, 42, 178, 1376, 1537, 3428, 3637
Bsp13I TCCGGA 1 cut(s) 1861
Bsp1407I TGTACA 1 cut(s) 1165
Bsp1720I GCTNAGC 1 cut(s) 2727
Bsp19I CCATGG 1 cut(s) 2854
BspANI GGCC 3 cut(s) 76, 3470, 3578
BspCNI CTCAG 7 cut(s) 30, 1116, 1477, 1483, 2740, 3103, 3541
BspEI TCCGGA 1 cut(s) 1861
BspFNI CGCG 2 cut(s) 114, 116
BspHI TCATGA 2 cut(s) 2314, 3228
BspLI GGNNCC 8 cut(s) 136, 163, 418, 763, 1534, 1930, 2935, 3219
BspMAI CTGCAG 4 cut(s) 1542, 1561, 1720, 3295
BspMI ACCTGC 1 cut(s) 3328
BspOI GCTAGC 1 cut(s) 1413
BspT104I TTCGAA 1 cut(s) 2687
BspT107I GGYRCC 4 cut(s) 161, 416, 761, 3217
BspTNI GGTCTC 1 cut(s) 1689
BsrDI GCAATG 5 cut(s) 703, 2767, 2913, 3402, 3555
BsrFI RCCGGY 2 cut(s) 2789, 3574
BsrGI TGTACA 1 cut(s) 1165
BssAI RCCGGY 2 cut(s) 2789, 3574
BssHII GCGCGC 1 cut(s) 114
BssNI GRCGYC 1 cut(s) 162
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 2 cut(s) 2854, 3408
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 4 cut(s) 581, 2871, 2948, 3631
Bst6I CTCTTC 6 cut(s) 1140, 1390, 2481, 2834, 3390, 3602
BstACI GRCGYC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 3554
BstAUI TGTACA 1 cut(s) 1165
BstBI TTCGAA 1 cut(s) 2687
BstC8I GCNNGC 7 cut(s) 24, 116, 160, 1411, 1439, 2758, 3576
BstDSI CCRYGG 1 cut(s) 2854
BstF5I GGATG 6 cut(s) 328, 388, 814, 2440, 2649, 3262
BstFNI CGCG 2 cut(s) 114, 116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 116, 118, 164, 3653
BstMAI GTCTC 6 cut(s) 193, 1387, 1481, 1689, 1905, 3112
BstMWI GCNNNNNNNGC 7 cut(s) 743, 1180, 1826, 2111, 2795, 3163, 3554
BstNI CCWGG 4 cut(s) 581, 2871, 2948, 3631
BstNSI RCATGY 3 cut(s) 1043, 1172, 1199
BstPAI GACNNNNGTC 1 cut(s) 1774
BstSCI CCNGG 8 cut(s) 229, 387, 388, 579, 2869, 2946, 3376, 3629
BstSFI CTRYAG 8 cut(s) 1538, 1557, 1716, 1743, 1915, 1959, 2367, 3291
BstSLI GKGCMC 4 cut(s) 42, 178, 1376, 3637
BstUI CGCG 2 cut(s) 114, 116
BstV2I GAAGAC 3 cut(s) 477, 536, 2577
BstX2I RGATCY 8 cut(s) 13, 217, 290, 1423, 2167, 2363, 3082, 3626
BstXI CCANNNNNNTGG 3 cut(s) 455, 1856, 2404
BstYI RGATCY 8 cut(s) 13, 217, 290, 1423, 2167, 2363, 3082, 3626
BsuRI GGCC 3 cut(s) 76, 3470, 3578
BtgI CCRYGG 1 cut(s) 2854
BtsCI GGATG 6 cut(s) 328, 388, 814, 2440, 2649, 3262
BtsIMutI CAGTG 6 cut(s) 765, 1374, 2044, 2668, 3426, 3494
BveI ACCTGC 1 cut(s) 3328
Cac8I GCNNGC 7 cut(s) 24, 116, 160, 1411, 1439, 2758, 3576
CaiI CAGNNNCTG 1 cut(s) 2171
CciI TCATGA 2 cut(s) 2314, 3228
CfoI GCGC 4 cut(s) 116, 118, 164, 3653
Cfr10I RCCGGY 2 cut(s) 2789, 3574
Cfr13I GGNCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
Cfr9I CCCGGG 1 cut(s) 388
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 1 cut(s) 120
Csp6I GTAC 2 cut(s) 1166, 3296
CspCI CAANNNNNGTGG 4 cut(s) 3021, 3056, 3320, 3355
CspI CGGWCCG 1 cut(s) 233
CviQI GTAC 2 cut(s) 1166, 3296
DinI GGCGCC 1 cut(s) 163
DraI TTTAAA 1 cut(s) 1507
DraIII CACNNNGTG 1 cut(s) 55
DrdI GACNNNNNNGTC 1 cut(s) 2581
DseDI GACNNNNNNGTC 1 cut(s) 2581
EaeI YGGCCR 1 cut(s) 3576
Eam1104I CTCTTC 6 cut(s) 1140, 1390, 2481, 2834, 3390, 3602
EarI CTCTTC 6 cut(s) 1140, 1390, 2481, 2834, 3390, 3602
EciI GGCGGA 1 cut(s) 291
Eco130I CCWWGG 2 cut(s) 2854, 3408
Eco147I AGGCCT 1 cut(s) 3470
Eco24I GRGCYC 2 cut(s) 28, 1537
Eco31I GGTCTC 1 cut(s) 1689
Eco32I GATATC 1 cut(s) 3584
Eco47I GGWCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
Eco57I CTGAAG 9 cut(s) 609, 687, 834, 912, 1041, 1626, 1785, 2943, 3573
Eco88I CYCGRG 5 cut(s) 94, 388, 2579, 3193, 3246
EcoO109I RGGNCCY 1 cut(s) 1928
EcoRI GAATTC 1 cut(s) 2689
EcoRII CCWGG 4 cut(s) 579, 2869, 2946, 3629
EcoRV GATATC 1 cut(s) 3584
EcoT14I CCWWGG 2 cut(s) 2854, 3408
EcoT22I ATGCAT 5 cut(s) 514, 739, 996, 1398, 2338
EcoT38I GRGCYC 2 cut(s) 28, 1537
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 2854, 3408
FaqI GGGAC 2 cut(s) 652, 998
FauI CCCGC 2 cut(s) 62, 1939
FbaI TGATCA 4 cut(s) 802, 1981, 2311, 3259
FokI GGATG 6 cut(s) 335, 395, 821, 2427, 2656, 3269
FriOI GRGCYC 2 cut(s) 28, 1537
FspBI CTAG 5 cut(s) 102, 842, 1410, 2640, 2694
GlaI GCGC 4 cut(s) 115, 117, 163, 3652
GsaI CCCAGC 1 cut(s) 2805
GsuI CTGGAG 2 cut(s) 1713, 1929
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 76, 3470, 3578
HapII CCGG 9 cut(s) 231, 366, 389, 1421, 1641, 1862, 2790, 3377, 3575
HgaI GACGC 1 cut(s) 120
HhaI GCGC 4 cut(s) 116, 118, 164, 3653
Hin1I GRCGYC 1 cut(s) 162
Hin6I GCGC 4 cut(s) 114, 116, 162, 3651
HinP1I GCGC 4 cut(s) 114, 116, 162, 3651
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HpaII CCGG 9 cut(s) 231, 366, 389, 1421, 1641, 1862, 2790, 3377, 3575
Hpy166II GTNNAC 5 cut(s) 176, 577, 704, 1810, 3043
Hpy8I GTNNAC 5 cut(s) 176, 577, 704, 1810, 3043
HpyF10VI GCNNNNNNNGC 7 cut(s) 743, 1180, 1826, 2111, 2795, 3163, 3554
Hsp92I GRCGYC 1 cut(s) 162
HspAI GCGC 4 cut(s) 114, 116, 162, 3651
KasI GGCGCC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 1861
KroI GCCGGC 1 cut(s) 3574
KroNI GCCGGC 1 cut(s) 3576
Ksp22I TGATCA 4 cut(s) 802, 1981, 2311, 3259
LmnI GCTCC 7 cut(s) 252, 970, 1532, 2647, 2939, 3423, 3529
MaeI CTAG 5 cut(s) 102, 842, 1410, 2640, 2694
MfeI CAATTG 1 cut(s) 3033
MflI RGATCY 8 cut(s) 13, 217, 290, 1423, 2167, 2363, 3082, 3626
MhlI GDGCHC 7 cut(s) 28, 42, 178, 1376, 1537, 3428, 3637
Mly113I GGCGCC 1 cut(s) 162
MmeI TCCRAC 2 cut(s) 357, 2451
Mph1103I ATGCAT 5 cut(s) 514, 739, 996, 1398, 2338
MroI TCCGGA 1 cut(s) 1861
MroNI GCCGGC 1 cut(s) 3574
MroXI GAANNNNTTC 3 cut(s) 954, 1346, 2553
MseI TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2217
MslI CAYNNNNRTG 7 cut(s) 96, 210, 453, 978, 1196, 1365, 2241
MspA1I CMGCKG 1 cut(s) 1979
MspI CCGG 9 cut(s) 231, 366, 389, 1421, 1641, 1862, 2790, 3377, 3575
MspR9I CCNGG 8 cut(s) 231, 389, 390, 581, 2871, 2948, 3378, 3631
MunI CAATTG 1 cut(s) 3033
Mva1269I GAATGC 3 cut(s) 439, 1156, 3166
MvaI CCWGG 4 cut(s) 581, 2871, 2948, 3631
MvnI CGCG 2 cut(s) 114, 116
MwoI GCNNNNNNNGC 7 cut(s) 743, 1180, 1826, 2111, 2795, 3163, 3554
NaeI GCCGGC 1 cut(s) 3576
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 4 cut(s) 231, 389, 390, 3378
NcoI CCATGG 1 cut(s) 2854
NgoMIV GCCGGC 1 cut(s) 3574
NheI GCTAGC 1 cut(s) 1409
NlaIV GGNNCC 8 cut(s) 136, 163, 418, 763, 1534, 1930, 2935, 3219
NmeAIII GCCGAG 2 cut(s) 190, 1407
NmuCI GTSAC 8 cut(s) 47, 59, 502, 538, 727, 817, 3372, 3412
NsiI ATGCAT 5 cut(s) 514, 739, 996, 1398, 2338
NspI RCATGY 3 cut(s) 1043, 1172, 1199
NspV TTCGAA 1 cut(s) 2687
OliI CACNNNNGTG 1 cut(s) 96
PagI TCATGA 2 cut(s) 2314, 3228
PauI GCGCGC 1 cut(s) 114
PceI AGGCCT 1 cut(s) 3470
PciI ACATGT 3 cut(s) 1039, 1168, 1195
PctI GAATGC 3 cut(s) 439, 1156, 3166
PdiI GCCGGC 1 cut(s) 3576
PdmI GAANNNNTTC 3 cut(s) 954, 1346, 2553
PluTI GGCGCC 1 cut(s) 165
PpuMI RGGWCCY 1 cut(s) 1928
PscI ACATGT 3 cut(s) 1039, 1168, 1195
PshAI GACNNNNGTC 1 cut(s) 1774
Psp5II RGGWCCY 1 cut(s) 1928
Psp6I CCWGG 4 cut(s) 579, 2869, 2946, 3629
PspFI CCCAGC 1 cut(s) 2801
PspGI CCWGG 4 cut(s) 579, 2869, 2946, 3629
PspN4I GGNNCC 8 cut(s) 136, 163, 418, 763, 1534, 1930, 2935, 3219
PspPI GGNCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
PspPPI RGGWCCY 1 cut(s) 1928
PstI CTGCAG 4 cut(s) 1542, 1561, 1720, 3295
PstNI CAGNNNCTG 1 cut(s) 2171
PsuI RGATCY 8 cut(s) 13, 217, 290, 1423, 2167, 2363, 3082, 3626
PteI GCGCGC 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 1979
RsaI GTAC 2 cut(s) 1167, 3297
RsaNI GTAC 2 cut(s) 1166, 3296
RseI CAYNNNNRTG 7 cut(s) 96, 210, 453, 978, 1196, 1365, 2241
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SaqAI TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2217
Sau96I GGNCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
ScrFI CCNGG 8 cut(s) 231, 389, 390, 581, 2871, 2948, 3378, 3631
SduI GDGCHC 7 cut(s) 28, 42, 178, 1376, 1537, 3428, 3637
SfcI CTRYAG 8 cut(s) 1538, 1557, 1716, 1743, 1915, 1959, 2367, 3291
SfoI GGCGCC 1 cut(s) 163
SfuI TTCGAA 1 cut(s) 2687
SinI GGWCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
SmaI CCCGGG 1 cut(s) 390
SmiMI CAYNNNNRTG 7 cut(s) 96, 210, 453, 978, 1196, 1365, 2241
SmlI CTYRAG 2 cut(s) 2433, 3472
SmoI CTYRAG 2 cut(s) 2433, 3472
SseBI AGGCCT 1 cut(s) 3470
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 1 cut(s) 2554
SspMI CTAG 5 cut(s) 102, 842, 1410, 2640, 2694
StuI AGGCCT 1 cut(s) 3470
StyD4I CCNGG 8 cut(s) 229, 387, 388, 579, 2869, 2946, 3376, 3629
StyI CCWWGG 2 cut(s) 2854, 3408
TaqI TCGA 2 cut(s) 2028, 2687
TaqII GACCGA 1 cut(s) 548
TatI WGTACW 2 cut(s) 1165, 3295
TauI GCSGC 1 cut(s) 2934
Tru1I TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2217
Tru9I TTAA 8 cut(s) 297, 566, 714, 750, 791, 1449, 1506, 2217
TscAI CASTG 6 cut(s) 772, 1381, 2044, 2668, 3433, 3501
TseFI GTSAC 8 cut(s) 47, 59, 502, 538, 727, 817, 3372, 3412
Tsp45I GTSAC 8 cut(s) 47, 59, 502, 538, 727, 817, 3372, 3412
TspGWI ACGGA 2 cut(s) 432, 3501
TspMI CCCGGG 1 cut(s) 388
TspRI CASTG 6 cut(s) 772, 1381, 2044, 2668, 3433, 3501
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 6 cut(s) 233, 363, 1859, 1928, 2851, 2944
XapI RAATTY 5 cut(s) 1998, 2155, 2504, 2689, 2712
XbaI TCTAGA 1 cut(s) 841
XceI RCATGY 3 cut(s) 1043, 1172, 1199
XmaI CCCGGG 1 cut(s) 388
XmnI GAANNNNTTC 3 cut(s) 954, 1346, 2553
XspI CTAG 5 cut(s) 102, 842, 1410, 2640, 2694
Zsp2I ATGCAT 5 cut(s) 514, 739, 996, 1398, 2338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.