Rh2DG353900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001352
Physical Location & Seq
Forward (+)
49552819 .. 49556288
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG353900.1

Sequence Viewer

Length: 696 bp
ATGGAGGGCAAACACCTGTTTTTGAAGCTGGCACAGTACCGGGTGCGGGTCAATCTTAACGATTCTGATCGGCAAAAGGCTGAGGAACGTACCCACCTCTTCATCAAAAGATTCCCTGAGTGGCAACGGAAAGCGATAATCCTTGGCATCGTCAGGACTACGAGAAGCCTCCTTAGACCTAGGTATATAGATGATGAGCTTATTCAAAAATTTATTGAAAAAATGAAGGAGTGGAAGAAGCTGGTGGTGAAGGATGATGATTTCGAGATGATGTCGGGGGATGAGGAGGAGGAGGAGGAGGAGGAGACGCTGTTGGAGGAGGGGGAAGAAGAACCGACAGAACAAGACAGGAGGGTGTGGTCGTACCTTTCCAGTAGCCTGGGATTCAGAATGAAGCCAGGAGAAGATGTCAAGATCGTCAGGCTGATGATTCCTTTCTTAGCCCAATGTTGTGGACGTCTTCCACCAGAAGGACCCGCGAGATGTGTCCACATCAAAATGTTCATACACCAGTTCCAAACATATATCATTAAATATTGCCTTAGCGACCTGAACGACGTGGAGATGCTGCTCGATGTCATTGCGGCTGTCGAGTCAAGTCCAGCAAATCCAGAAAATAAGTTAAAGAAAAATATACCAACTAAAGAAGAAGGGATTGCTGCCCCCATGTACATCTTAAAACTAGAGAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

27.27

Weight (kDa)

5.62

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 460
AccII CGCG 1 cut(s) 479
AciI CCGC 3 cut(s) 46, 477, 584
AcsI RAATTY 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 457
AfaI GTAC 4 cut(s) 38, 91, 365, 671
AfiI CCNNNNNNNGG 2 cut(s) 46, 470
AgsI TTSAA 3 cut(s) 25, 206, 218
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 378, 397
AluBI AGCT 3 cut(s) 28, 199, 241
AluI AGCT 3 cut(s) 28, 199, 241
Alw26I GTCTC 1 cut(s) 299
ApeKI GCWGC 2 cut(s) 568, 659
ApoI RAATTY 1 cut(s) 209
AspA2I CCTAGG 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 1 cut(s) 473
AvrII CCTAGG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 452
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 555, 646
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 2 cut(s) 380, 399
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 180, 683
BisI GCNGC 3 cut(s) 569, 585, 660
BlnI CCTAGG 1 cut(s) 179
BlsI GCNGC 3 cut(s) 570, 586, 661
Bme1390I CCNGG 3 cut(s) 41, 380, 399
Bme18I GGWCC 1 cut(s) 473
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 3 cut(s) 41, 380, 399
BmsI GCATC 2 cut(s) 156, 555
BpiI GAAGAC 1 cut(s) 452
Bpu10I CCTNAGC 2 cut(s) 81, 542
BpuMI CCSGG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 457
BsaJI CCNNGG 3 cut(s) 142, 179, 379
BsaXI ACNNNNNCTCC 4 cut(s) 296, 326, 393, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 470
Bse1I ACTGG 2 cut(s) 372, 511
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 2 cut(s) 380, 399
BseDI CCNNGG 3 cut(s) 142, 179, 379
BseGI GGATG 2 cut(s) 259, 286
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 46, 470
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 72, 108
BseNI ACTGG 2 cut(s) 372, 511
BseRI GAGGAG 8 cut(s) 299, 302, 305, 308, 311, 314, 317, 332
BseXI GCAGC 2 cut(s) 555, 646
Bsh1236I CGCG 1 cut(s) 479
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 2 cut(s) 46, 470
BsmAI GTCTC 1 cut(s) 299
BsmBI CGTCTC 1 cut(s) 299
Bsp1407I TGTACA 1 cut(s) 669
Bsp143I GATC 2 cut(s) 67, 414
BspACI CCGC 3 cut(s) 46, 477, 584
BspCNI CTCAG 2 cut(s) 73, 109
BspFNI CGCG 1 cut(s) 479
BspLI GGNNCC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 579
BsrGI TGTACA 1 cut(s) 669
BsrI ACTGG 2 cut(s) 372, 511
BssECI CCNNGG 3 cut(s) 142, 179, 379
BssMI GATC 2 cut(s) 67, 414
BssNI GRCGYC 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 142, 179
Bst2UI CCWGG 2 cut(s) 380, 399
Bst4CI ACNGT 1 cut(s) 36
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 1 cut(s) 457
BstAUI TGTACA 1 cut(s) 669
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 5 cut(s) 81, 117, 173, 439, 542
BstF5I GGATG 2 cut(s) 259, 286
BstFNI CGCG 1 cut(s) 479
BstKTI GATC 2 cut(s) 70, 417
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 2 cut(s) 67, 414
BstNI CCWGG 2 cut(s) 380, 399
BstSCI CCNGG 3 cut(s) 39, 378, 397
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 2 cut(s) 555, 646
BstV2I GAAGAC 1 cut(s) 452
BstXI CCANNNNNNTGG 2 cut(s) 379, 452
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 2 cut(s) 259, 286
Cac8I GCNNGC 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 473
CseI GACGC 1 cut(s) 316
Csp6I GTAC 4 cut(s) 37, 90, 364, 670
CviAII CATG 1 cut(s) 667
CviQI GTAC 4 cut(s) 37, 90, 364, 670
DdeI CTNAG 5 cut(s) 81, 117, 173, 439, 542
DpnI GATC 2 cut(s) 69, 416
DpnII GATC 2 cut(s) 67, 414
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco130I CCWWGG 2 cut(s) 142, 179
Eco47I GGWCC 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 378, 397
EcoT14I CCWWGG 2 cut(s) 142, 179
ErhI CCWWGG 2 cut(s) 142, 179
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 1 cut(s) 670
FaiI YATR 7 cut(s) 186, 188, 506, 523, 525, 635, 668
FatI CATG 1 cut(s) 666
FauI CCCGC 2 cut(s) 39, 484
Fnu4HI GCNGC 3 cut(s) 569, 585, 660
FokI GGATG 2 cut(s) 266, 293
Fsp4HI GCNGC 3 cut(s) 569, 585, 660
FspBI CTAG 2 cut(s) 180, 683
GluI GCNGC 3 cut(s) 569, 585, 660
HapII CCGG 1 cut(s) 40
HgaI GACGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 457
Hin1II CATG 1 cut(s) 670
HinfI GANTC 5 cut(s) 62, 111, 384, 430, 593
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 455, 490
Hpy188I TCNGA 2 cut(s) 67, 389
Hpy188III TCNNGA 4 cut(s) 154, 265, 412, 611
Hpy8I GTNNAC 2 cut(s) 455, 490
Hpy99I CGWCG 1 cut(s) 560
HpyAV CCTTC 5 cut(s) 220, 244, 464, 644, 684
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4IV ACGT 3 cut(s) 88, 457, 558
HpyF3I CTNAG 5 cut(s) 81, 117, 173, 439, 542
HpySE526I ACGT 3 cut(s) 88, 457, 558
Hsp92I GRCGYC 1 cut(s) 457
Hsp92II CATG 1 cut(s) 670
Kzo9I GATC 2 cut(s) 67, 414
Lsp1109I GCAGC 2 cut(s) 555, 646
LweI GCATC 2 cut(s) 156, 555
MaeI CTAG 2 cut(s) 180, 683
MaeII ACGT 3 cut(s) 88, 457, 558
MalI GATC 2 cut(s) 69, 416
MboI GATC 2 cut(s) 67, 414
MboII GAAGA 7 cut(s) 91, 247, 338, 341, 416, 452, 659
MluCI AATT 1 cut(s) 209
MlyI GAGTC 1 cut(s) 602
MmeI TCCRAC 1 cut(s) 294
MseI TTAA 4 cut(s) 57, 531, 623, 677
MslI CAYNNNNRTG 1 cut(s) 497
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 3 cut(s) 41, 380, 399
MvaI CCWGG 2 cut(s) 380, 399
MvnI CGCG 1 cut(s) 479
NciI CCSGG 1 cut(s) 41
NdeII GATC 2 cut(s) 67, 414
NlaIII CATG 1 cut(s) 670
NlaIV GGNNCC 1 cut(s) 475
PfeI GAWTC 4 cut(s) 62, 111, 384, 430
PkrI GCNGC 3 cut(s) 570, 586, 661
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PpuMI RGGWCCY 1 cut(s) 473
Psp5II RGGWCCY 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 378, 397
PspGI CCWGG 2 cut(s) 378, 397
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 1 cut(s) 473
PspPPI RGGWCCY 1 cut(s) 473
RsaI GTAC 4 cut(s) 38, 91, 365, 671
RsaNI GTAC 4 cut(s) 37, 90, 364, 670
RseI CAYNNNNRTG 1 cut(s) 497
SaqAI TTAA 4 cut(s) 57, 531, 623, 677
SatI GCNGC 3 cut(s) 569, 585, 660
Sau3AI GATC 2 cut(s) 67, 414
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 1 cut(s) 602
ScrFI CCNGG 3 cut(s) 41, 380, 399
SfaNI GCATC 2 cut(s) 156, 555
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 1 cut(s) 209
SsiI CCGC 3 cut(s) 46, 477, 584
SspI AATATT 1 cut(s) 536
SspMI CTAG 2 cut(s) 180, 683
StyD4I CCNGG 3 cut(s) 39, 378, 397
StyI CCWWGG 2 cut(s) 142, 179
TaaI ACNGT 1 cut(s) 36
TaiI ACGT 3 cut(s) 91, 460, 561
TaqI TCGA 3 cut(s) 264, 573, 591
TasI AATT 1 cut(s) 209
TatI WGTACW 1 cut(s) 669
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 4 cut(s) 62, 111, 384, 430
Tru1I TTAA 4 cut(s) 57, 531, 623, 677
Tru9I TTAA 4 cut(s) 57, 531, 623, 677
TseI GCWGC 2 cut(s) 568, 659
TspDTI ATGAA 4 cut(s) 91, 239, 407, 493
TspGWI ACGGA 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 473
XapI RAATTY 1 cut(s) 209
XmaJI CCTAGG 1 cut(s) 179
XspI CTAG 2 cut(s) 180, 683
ZraI GACGTC 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.