Rh2AG281100

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001352
Physical Location & Seq
Reverse (-)
34157673 .. 34160998
3326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG281100.1

Sequence Viewer

Length: 1440 bp
ATGATGTCGGGGGATGAAGAGGAGGAGGAGGAGGAGGAGACGCTGTTGGAGGAGGAGGAGGGGGAAGAAGAACCGACAGAACAAGACAGGAGGGTGTGGTCGTACCTTTCCAGTAGCCTGGGATTCAGAATGAAGCCAGGAGAAGATGTCAAGATCGTCAGGCTGATGATTCCTTTCTTAGCCCAATGTTGTGGACCTCTTCCACCAGAAGGACCCGCGAGATGTGTCCACATCAAAATGTTCATACACCAGTTCCAAACATATATCATTAAATATTGCCTTAGCGACCTGAACGACGTGGAGATGCTGCTCGATGTCATTAAGGCTGTCGAGTCAAGTCCAGCAAATCCAGAAAATAAGTTAAAGAAAAATATCGTACCAACTAAAGAAGAAGGGATTGCTGCCCCCATGTACATCTTAAAACTAGAGAGAAGAGAATATGGAGACCACAGCAGAAAAGGACCTGCAGAAGAAAATCTTGTGGCAAATCCTATAGCTATTGTTCAATCTGCTGAAGGACAGGAATCTTATGAGAAGTCCAATGAGTCTTTACAGGTGAACTTTGCTGCCAGTGATGCTGGTATAGATCTAGCTACCCACTCAGTTGGTCTGGATGATAATGAAAAAGATGGAAACTGTGATGGGAGTGGAGAGTTTGGAGACTACAGCAGAAAAGGACCTGCAGAAGAAAATCTTGTGGCAACTCCTATAGCTACTGTTCAATCAGCTGATCATCATGAAAACCAAATTTATCCAGCAGCTAACTCACACAAAGGTGATGATGGCGGTGATTATGAGAAAGGCGTAATTGAAAAGTGTAATAAAATTGGAGATGCCGGTTTCAATGAAAAGGCTGAAACCTCTAACTTATGTGGGGATGACAAAAGCAAAGGAGCTACAGAGAATTTTTCCACAGATCCAGCAAGTAATTTGGAATCTGTCAGTCGTCTATCTGAACTCCAAGTTAATCCTGTAACCAATCTACATGAAAGTGGTGATGCTGCTGGTGATTGTGAGAAGGATAAAACCAAAAAGTGTGATATAATTGGGTATGATGGTGATCATGAGAAAGCTGAAAAGTATGCATCATCTGGTGAAGACATAAGTAAAAGCTCTACAGAAAAAACCTTTGCGGGGGACTCTACAACTAATTTGGAATCCATAATTACTCTCTCGGACTTTCAAGTTCATCCAGCAGAAAAGTTGCCTGATGTTGATGGTTCTGCTAATCATGATAAGGGTGAAATTGATAAATTTGTTATACCTGGAAATGAAAGCACGGAAGGATCTCTTGAAGAAAATATTCTGGTGAAGTCAGAATTGACCCCCGAGTCTTCTGTCAATCTTACTGGTTCCAAAGCTGTTGGTGAAGATGCAGCCGATGGTTCTAGGATGGAGCTGGTGGAAACCAGTGGCAGAGATTTAGAGAGATTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

479

Amino Acids

52.21

Weight (kDa)

4.46

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1330
Acc36I ACCTGC 2 cut(s) 472, 688
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 216, 786, 1133
AclWI GGATC 2 cut(s) 911, 1294
AcsI RAATTY 3 cut(s) 747, 904, 1253
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 3 cut(s) 104, 378, 413
AfiI CCNNNNNNNGG 2 cut(s) 209, 1134
AgsI TTSAA 6 cut(s) 506, 722, 812, 844, 1184, 1295
AjiI CACGTC 1 cut(s) 298
AjnI CCWGG 3 cut(s) 117, 136, 1264
AleI CACNNNNGTG 1 cut(s) 774
Alw26I GTCTC 3 cut(s) 32, 438, 654
AlwI GGATC 2 cut(s) 911, 1294
Ama87I CYCGRG 1 cut(s) 1328
ApeKI GCWGC 6 cut(s) 307, 401, 566, 758, 1001, 1376
ApoI RAATTY 3 cut(s) 747, 904, 1253
Asp700I GAANNNNTTC 1 cut(s) 1302
AspS9I GGNCC 4 cut(s) 194, 212, 461, 677
AvaI CYCGRG 1 cut(s) 1328
AvaII GGWCC 4 cut(s) 194, 212, 461, 677
BbsI GAAGAC 2 cut(s) 1104, 1326
BbvI GCAGC 6 cut(s) 294, 388, 553, 770, 988, 1388
BccI CCATC 7 cut(s) 623, 635, 776, 1049, 1211, 1376, 1387
BciT130I CCWGG 3 cut(s) 119, 138, 1266
BclI TGATCA 2 cut(s) 730, 1060
BcoDI GTCTC 3 cut(s) 32, 438, 654
BfaI CTAG 3 cut(s) 425, 590, 1389
BfmI CTRYAG 7 cut(s) 465, 492, 664, 681, 708, 897, 1116
BfuAI ACCTGC 2 cut(s) 472, 688
BglII AGATCT 1 cut(s) 586
BisI GCNGC 6 cut(s) 308, 402, 567, 759, 1002, 1377
BlsI GCNGC 6 cut(s) 309, 403, 568, 760, 1003, 1378
Bme1390I CCNGG 3 cut(s) 119, 138, 1266
Bme18I GGWCC 4 cut(s) 194, 212, 461, 677
BmeT110I CYCGRG 1 cut(s) 1328
BmgBI CACGTC 1 cut(s) 298
BmgT120I GGNCC 4 cut(s) 194, 212, 461, 677
BmiI GGNNCC 2 cut(s) 214, 1354
BmrFI CCNGG 3 cut(s) 119, 138, 1266
BmsI GCATC 6 cut(s) 294, 565, 823, 988, 1094, 1363
BpiI GAAGAC 2 cut(s) 1104, 1326
Bpu10I CCTNAGC 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 1059
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 118
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 132, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 1134
Bse118I RCCGGY 1 cut(s) 836
Bse1I ACTGG 5 cut(s) 111, 250, 570, 1354, 1410
Bse8I GATNNNNATC 1 cut(s) 1059
BseBI CCWGG 3 cut(s) 119, 138, 1266
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 5 cut(s) 19, 619, 883, 1189, 1398
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 2 cut(s) 209, 1134
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 5 cut(s) 111, 250, 570, 1354, 1410
BseRI GAGGAG 9 cut(s) 35, 38, 41, 44, 47, 50, 65, 68, 71
BseXI GCAGC 6 cut(s) 294, 388, 553, 770, 988, 1388
Bsh1236I CGCG 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 1 cut(s) 837
BslFI GGGAC 1 cut(s) 1151
BslI CCNNNNNNNGG 2 cut(s) 209, 1134
BsmAI GTCTC 3 cut(s) 32, 438, 654
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 1151
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 1328
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
BspACI CCGC 3 cut(s) 216, 786, 1133
BspCNI CTCAG 1 cut(s) 614
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 3 cut(s) 736, 1063, 1231
BspLI GGNNCC 2 cut(s) 214, 1354
BspMAI CTGCAG 2 cut(s) 469, 685
BspMI ACCTGC 2 cut(s) 472, 688
BspPI GGATC 2 cut(s) 911, 1294
BspTNI GGTCTC 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 836
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 5 cut(s) 111, 250, 570, 1354, 1410
BssAI RCCGGY 1 cut(s) 836
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
Bst2UI CCWGG 3 cut(s) 119, 138, 1266
Bst4CI ACNGT 2 cut(s) 638, 718
Bst6I CTCTTC 3 cut(s) 12, 204, 427
BstAUI TGTACA 1 cut(s) 411
BstDEI CTNAG 3 cut(s) 178, 281, 601
BstF5I GGATG 5 cut(s) 19, 619, 883, 1189, 1398
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 6 cut(s) 156, 589, 733, 919, 1063, 1289
BstMAI GTCTC 3 cut(s) 32, 438, 654
BstMBI GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
BstMWI GCNNNNNNNGC 1 cut(s) 575
BstNI CCWGG 3 cut(s) 119, 138, 1266
BstSCI CCNGG 3 cut(s) 117, 136, 1264
BstSFI CTRYAG 7 cut(s) 465, 492, 664, 681, 708, 897, 1116
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 6 cut(s) 294, 388, 553, 770, 988, 1388
BstV2I GAAGAC 2 cut(s) 1104, 1326
BstX2I RGATCY 3 cut(s) 586, 916, 1286
BstXI CCANNNNNNTGG 3 cut(s) 118, 191, 605
BstYI RGATCY 3 cut(s) 586, 916, 1286
BtrI CACGTC 1 cut(s) 298
BtsCI GGATG 5 cut(s) 19, 619, 883, 1189, 1398
BtsIMutI CAGTG 2 cut(s) 577, 1417
BveI ACCTGC 2 cut(s) 472, 688
CciI TCATGA 3 cut(s) 736, 1063, 1231
Cfr10I RCCGGY 1 cut(s) 836
Cfr13I GGNCC 4 cut(s) 194, 212, 461, 677
CseI GACGC 1 cut(s) 49
Csp6I GTAC 3 cut(s) 103, 377, 412
CviAII CATG 5 cut(s) 409, 737, 986, 1064, 1232
CviQI GTAC 3 cut(s) 103, 377, 412
DdeI CTNAG 3 cut(s) 178, 281, 601
DpnI GATC 6 cut(s) 155, 588, 732, 918, 1062, 1288
DpnII GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
DrdI GACNNNNNNGTC 1 cut(s) 1330
DseDI GACNNNNNNGTC 1 cut(s) 1330
Eam1104I CTCTTC 3 cut(s) 12, 204, 427
EarI CTCTTC 3 cut(s) 12, 204, 427
Eco31I GGTCTC 1 cut(s) 438
Eco47I GGWCC 4 cut(s) 194, 212, 461, 677
Eco57I CTGAAG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 1328
EcoO109I RGGNCCY 3 cut(s) 212, 461, 677
EcoRII CCWGG 3 cut(s) 117, 136, 1264
EcoT22I ATGCAT 1 cut(s) 1087
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 5 cut(s) 412, 740, 989, 1067, 1235
FalI AAGNNNNNCTT 6 cut(s) 462, 494, 678, 710, 1275, 1307
FaqI GGGAC 1 cut(s) 1151
FatI CATG 5 cut(s) 408, 736, 985, 1063, 1231
FauI CCCGC 2 cut(s) 223, 1126
FbaI TGATCA 2 cut(s) 730, 1060
Fnu4HI GCNGC 6 cut(s) 308, 402, 567, 759, 1002, 1377
FokI GGATG 5 cut(s) 26, 626, 890, 1176, 1405
Fsp4HI GCNGC 6 cut(s) 308, 402, 567, 759, 1002, 1377
FspBI CTAG 3 cut(s) 425, 590, 1389
GluI GCNGC 6 cut(s) 308, 402, 567, 759, 1002, 1377
HapII CCGG 1 cut(s) 837
HgaI GACGC 1 cut(s) 49
Hin1II CATG 5 cut(s) 412, 740, 989, 1067, 1235
HinfI GANTC 9 cut(s) 123, 169, 332, 524, 545, 935, 1139, 1157, 1331
HpaII CCGG 1 cut(s) 837
Hpy166II GTNNAC 3 cut(s) 194, 229, 559
Hpy188I TCNGA 4 cut(s) 128, 955, 1177, 1318
Hpy188III TCNNGA 7 cut(s) 151, 350, 611, 737, 1064, 1232, 1292
Hpy8I GTNNAC 3 cut(s) 194, 229, 559
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 5 cut(s) 203, 386, 509, 1012, 1277
HpyCH4III ACNGT 2 cut(s) 638, 718
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 4 cut(s) 467, 683, 1085, 1376
HpyF10VI GCNNNNNNNGC 1 cut(s) 575
HpyF3I CTNAG 3 cut(s) 178, 281, 601
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 5 cut(s) 412, 740, 989, 1067, 1235
Ksp22I TGATCA 2 cut(s) 730, 1060
Kzo9I GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
LmnI GCTCC 2 cut(s) 893, 1396
Lsp1109I GCAGC 6 cut(s) 294, 388, 553, 770, 988, 1388
LweI GCATC 6 cut(s) 294, 565, 823, 988, 1094, 1363
MaeI CTAG 3 cut(s) 425, 590, 1389
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 973
MalI GATC 6 cut(s) 155, 588, 732, 918, 1062, 1288
MboI GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
MflI RGATCY 3 cut(s) 586, 916, 1286
MlyI GAGTC 4 cut(s) 341, 554, 1133, 1340
MmeI TCCRAC 1 cut(s) 27
Mph1103I ATGCAT 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 1302
MseI TTAA 5 cut(s) 270, 321, 362, 419, 966
MslI CAYNNNNRTG 3 cut(s) 236, 774, 990
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 1 cut(s) 837
MspR9I CCNGG 3 cut(s) 119, 138, 1266
MvaI CCWGG 3 cut(s) 119, 138, 1266
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 575
NdeII GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
NlaIII CATG 5 cut(s) 412, 740, 989, 1067, 1235
NlaIV GGNNCC 2 cut(s) 214, 1354
NsiI ATGCAT 1 cut(s) 1087
OliI CACNNNNGTG 1 cut(s) 774
PagI TCATGA 3 cut(s) 736, 1063, 1231
PdmI GAANNNNTTC 1 cut(s) 1302
PfeI GAWTC 5 cut(s) 123, 169, 524, 935, 1157
PkrI GCNGC 6 cut(s) 309, 403, 568, 760, 1003, 1378
PleI GAGTC 4 cut(s) 340, 553, 1133, 1339
PpsI GAGTC 4 cut(s) 340, 553, 1133, 1339
PpuMI RGGWCCY 3 cut(s) 212, 461, 677
Psp5II RGGWCCY 3 cut(s) 212, 461, 677
Psp6I CCWGG 3 cut(s) 117, 136, 1264
PspGI CCWGG 3 cut(s) 117, 136, 1264
PspN4I GGNNCC 2 cut(s) 214, 1354
PspPI GGNCC 4 cut(s) 194, 212, 461, 677
PspPPI RGGWCCY 3 cut(s) 212, 461, 677
PstI CTGCAG 2 cut(s) 469, 685
PsuI RGATCY 3 cut(s) 586, 916, 1286
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 3 cut(s) 104, 378, 413
RsaNI GTAC 3 cut(s) 103, 377, 412
RseI CAYNNNNRTG 3 cut(s) 236, 774, 990
SaqAI TTAA 5 cut(s) 270, 321, 362, 419, 966
SatI GCNGC 6 cut(s) 308, 402, 567, 759, 1002, 1377
Sau3AI GATC 6 cut(s) 153, 586, 730, 916, 1060, 1286
Sau96I GGNCC 4 cut(s) 194, 212, 461, 677
SchI GAGTC 4 cut(s) 341, 554, 1133, 1340
ScrFI CCNGG 3 cut(s) 119, 138, 1266
SfaNI GCATC 6 cut(s) 294, 565, 823, 988, 1094, 1363
SfcI CTRYAG 7 cut(s) 465, 492, 664, 681, 708, 897, 1116
SinI GGWCC 4 cut(s) 194, 212, 461, 677
SmiMI CAYNNNNRTG 3 cut(s) 236, 774, 990
SsiI CCGC 3 cut(s) 216, 786, 1133
SspI AATATT 2 cut(s) 275, 1303
SspMI CTAG 3 cut(s) 425, 590, 1389
StyD4I CCNGG 3 cut(s) 117, 136, 1264
TaaI ACNGT 2 cut(s) 638, 718
TaiI ACGT 1 cut(s) 300
TaqI TCGA 2 cut(s) 312, 330
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 5 cut(s) 123, 169, 524, 935, 1157
Tru1I TTAA 5 cut(s) 270, 321, 362, 419, 966
Tru9I TTAA 5 cut(s) 270, 321, 362, 419, 966
TscAI CASTG 2 cut(s) 577, 1417
TseI GCWGC 6 cut(s) 307, 401, 566, 758, 1001, 1376
TspDTI ATGAA 9 cut(s) 30, 146, 232, 636, 753, 861, 1002, 1178, 1287
TspGWI ACGGA 1 cut(s) 1295
TspRI CASTG 2 cut(s) 577, 1417
VpaK11BI GGWCC 4 cut(s) 194, 212, 461, 677
XapI RAATTY 3 cut(s) 747, 904, 1253
XmnI GAANNNNTTC 1 cut(s) 1302
XspI CTAG 3 cut(s) 425, 590, 1389
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.