Rroxscaffold_5G00356750

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001352
Physical Location & Seq
Reverse (-)
36129417 .. 36135530
6114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356750.1

Sequence Viewer

Length: 3546 bp
ATGGATCACCAAGATCTCAGGCGAGCCCACAGTTCAGGGCACGAGAGTCACCATGTGTGTCACAAATGCGGGTGGCCTTTTCCGAATCCGCACCCGAGTGCTAGACACAGGCGCGCGCATAAGAAGATTTGCGGAACCGTTGAGGGTTACAAGGTGGCTGGCGCTGAGGGGAGTGCACAGTTGAGTGTCTCTGATGATGAGCAGCACTCTGATGAAGATCCCAAAACCCCCGGTCCGAAAGTCTTGGAAAGGAGCGTTTATGAGAGGGGTAGTGGCGTAATTGGGCAAGGATCTATTAAATCAGATGATGGGGTGTTCTCGGATGCTGTTCAGGAGTTTTCAGATAGCGGTTCTGCTGCATGGACCAGAGAAGGGTTTGAGGATGCCCCAGGATCAGCACCTAATGTAGAAAGGGGTGCCAAGAGTGATTTGAATGCCATTCCGTTCCACAATGATGGGGGAACGACAGACAAATCAAACACCAAAAAATATGAAACGGTGACAGATGCATCAGAAGATATGAAGACCGATGTGACTGACAGCATGATTGAGAGCTGCTTAACATCTGTTGATCAGGAAGCTGAAGTGACAGGAATTACAGCAACTAATCTGGATAGGAACATCTGTTCAGAGACTACTTCTATTTCAGATGGTAGAACTGAAGAATCAAATGTGCTTCTTGGTGATAGCTATGGTTTACACCCCATTAAATATGAAATGGTGACAGATGCATCAGAAGCTATTAAAAAGACTGGTGCCACTGACAGCATGATTGAGAGCTGCTTGACATCTGTTGATCAGGATGCTGAAGTGACGGGAATTACAGCAACTAATCTAGATAGTAACATAATCTTACCCAATAAAGTTGCTGGTGAAACAGTTGAGGGTGGCTCTAAGTTAGAAGGATCAAATGAAATGATTTCAGAATATGTGCGAGATGATGAACTTGTTCCATCAGAGAAGGAGCATACTGATGGGTTTGATATGCATATTCCACAAATTGATGTCCCCCCTGAAATAGAATCTGTTGCACATGTGAATACTTTTGTTGATACTGCTGAAAAGAAGGTGGATAATACAGATGGAATACAGTATCTCAGTCCTGATAAAGTGATAGAGAGTCGTAATGGGAAGGAGGAAGAGAATGCAAATGCAGATGTACATGTGCTTTCTGTGCCTGATGACATACATGTGGTAGATGATCCTGTGCTGATGCTTGAAGATTTCAGAAACCATAAAGGAAAGCAATTAGACCAAGTTACTTTCTTGGACTCATTGGAACAGTTTGATGATAAGGAAAATGATGTCACACAACCTGTTTTCAACGAGAGTCTCAACATTTTCCAGTCAAGTCCTCTAAGTGGAGGCACTGTTGTCTCTTCTGATATGCATGATTTGGAAGCCAGCCTTGAACCGGATCTCGGCAGCAGCAAGCCAATGATTAAAAGTGTGCCTGTTGAAGATGAAGCCAATATGTCTGAGTCCGAGATCAAGATGTTTAAAAACCGAAGTTCAAATGAAATAGGAGCCCCTGCAGATGCTGTGATGCCTCAGATAGCTAAAACCTATATGGTTGGTTTACCTGAAGGACATGAATCTTATGATAAGTCCAATGAGTCTTTATGGGTGAACTTTGCTGCCAGTGATGCTGGTATAGATCAAACAACCCACTCAGTTGGTCCAGAGAATAATGAAAAAGATGGAAACTCTGATGGGACTGGAGAGTCTGGAGACCACAGCAGAAAAGTAACTGCAGAAGAAAATCTTGTGGCAAATCCTATAGCTACTGTTCAATCTGCTGAAGTACGGAAATCTGATGAGAAGTCCAATGAGTCTTTACAGGTGAACTTTGCTGCCAGTGATGCTGGTATAGATCAAACTACCCACTCAGTTGGTCCGGACGATAATGAAAAAGATGGAAACTGTGATGGGAGTGGAGAATCTGGAGACTACAGCAGAAACGGACCCGCAGAAGAAAATCTTGTGGCAACTCCTATAGCTACTGTTCAATCAGCTGATCATTATGAAAACCAAATTTATCCAGCAGTTAACTTACACAAAGTTGATGATGCCGGTGAGTATGAGAAGGGCGTAATTGACAAGTGTAATAAAATTGGAGATGCTGGTTACAATGAAAACGCTGAAACCTCTAACTTATGTGGGGATGACAAAAGCAAAGGAGTTGCAGAGAATTTTTCCACAGATCCTGCAAGTAATTTGGAATCTATTAGTTGTCTATCTGAGCTCCAAGTTAATCCAGTAACCAATCTACATGAAAGTGGTGATGCTGCTGGAGATTGTGAGAAGGATAAAACCGAAAAGTGTGATATCACTGGGTATGATGGTGATCATGAGAAAGCTGAAAAGTATGCATCATCTGGTGAAGAAATAAGTAAAAGATCTACAGAAAAAACCTTTGCGGAGGACTCTACCACTAATTTGGAATCCATCAGTACTCTCTCCAACTCTCAAGTTCATCCAGCGGGAAAGTTGCCTGATGTTGATGGCTCTGCTGATCATGATGAGGGTGAAATTGAGAAATTTGTTATATCTGGAAATGAAAGCATGGAAGGATCAGTTGAAGAAAATATTCTGGTGAAGTCAGAATTGACCCCCGAGTCTTCTGCCAATCTAACTGGTTCAAAAGCTATTGTTGAAGATGCAGCCAATGGTTCTAGGATGGAGCTGGTTGAAACCAGTGGCAGAGATTTAGATAGCGGTTCGAATTCTAGCAAAGTTGCAGGAAAAATTCTATCAGATGCTCAGCAAGATGTGTTAGAAACTGAGATTGCTTGCAATGACAAAATCCTGAAAGAAGTTGATGCTGGTGATGCTGCTGGGTCAGATCATTGTCAGAATGTTGAATCGTTCCAGAAGAGTTCGGAGGACCATGGGAAGAAAGAACCAGGGTTTTCTGCTTTAGATACCGAATCTTCTGTTCAAAGTTTCATTGCCTCTGAAGATAGCGGCTCCCAAGAGGTCCATGCGGTTGCTTCTAGGATTACTTCTCAATCTTTGTTAGAGGAAGGTGATAATAACCTTGTTGGACAACAGCCAAGCTCAACTGCAATTGACATGGACTCAAATAGCCAAACTGACAGTCTGGAAGCCAACTGGGGATCTTTCTCAGTTCTTTCCATGCAATCTGATGCTGAAACTGTGCCACATACGCATTCCCAATCTTCATTAGGCGAAAACTCTGACTTGATGAAGTCAAAATTTGAATGTGAGCGTCAATACTTGGACAAATCTGATATGTTTGAGGCACCATCTTTCATGACATTGGTTGAACCCCGAGGTGGGGATGATAAAAAAGCTACTGGTTCTGAAATCCTGACTGCAGTACAGAACCCAGAACAGCCAAAACCTGCATCTTTGCAAACTGGGTGGTTTCCTTCTATGATCCATGTTGTGAATGAGTCACCGGGGAGAAAGAAGAACGAAGAGATCATTGCCAAGGTGACAAACAGGAGCACTGGGAAGCAACACATTCCTTTGAAAAACCTTTTGGGGAGGCCTCAAGTGAAAGCAACGGAAAGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1181

Amino Acids

127.02

Weight (kDa)

4.45

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1723, 2617
Acc36I ACCTGC 1 cut(s) 3376
AccB1I GGYRCC 3 cut(s) 416, 755, 3265
AccII CGCG 2 cut(s) 114, 116
AccIII TCCGGA 1 cut(s) 1897
AcsI RAATTY 6 cut(s) 2034, 2191, 2540, 2725, 2748, 3218
AcuI CTGAAG 6 cut(s) 603, 681, 828, 1605, 1821, 2979
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 4 cut(s) 1161, 1806, 2455, 3345
AfiI CCNNNNNNNGG 7 cut(s) 372, 1361, 1414, 1421, 3299, 3300, 3301
AflIII ACRYGT 3 cut(s) 1033, 1162, 1189
AjnI CCWGG 2 cut(s) 388, 2905
AjuI GAANNNNNNNTTGG 2 cut(s) 3491, 3523
AleI CACNNNNGTG 1 cut(s) 96
AloI GAACNNNNNNTCC 2 cut(s) 610, 642
Alw21I GWGCWC 3 cut(s) 178, 2247, 3476
Alw26I GTCTC 6 cut(s) 193, 626, 1337, 1381, 1725, 1941
Alw44I GTGCAC 1 cut(s) 174
AlwNI CAGNNNCTG 2 cut(s) 1541, 2207
Ama87I CYCGRG 3 cut(s) 94, 2615, 3294
Aor13HI TCCGGA 1 cut(s) 1897
AoxI GGCC 2 cut(s) 74, 3515
ApaLI GTGCAC 1 cut(s) 174
ApoI RAATTY 6 cut(s) 2034, 2191, 2540, 2725, 2748, 3218
ArsI GACNNNNNNTTYG 2 cut(s) 3085, 3117
Asp700I GAANNNNTTC 2 cut(s) 948, 2589
AspLEI GCGC 4 cut(s) 114, 116, 118, 164
AspS9I GGNCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
AsuC2I CCSGG 2 cut(s) 231, 3426
AsuII TTCGAA 1 cut(s) 2723
AvaI CYCGRG 3 cut(s) 94, 2615, 3294
AvaII GGWCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
BaeGI GKGCMC 2 cut(s) 42, 178
BanI GGYRCC 3 cut(s) 416, 755, 3265
BanII GRGCYC 3 cut(s) 28, 1531, 2247
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 2 cut(s) 530, 2613
Bbv12I GWGCWC 3 cut(s) 178, 2247, 3476
BbvCI CCTCAGC 1 cut(s) 165
BcgI CGANNNNNNTGC 2 cut(s) 2606, 2640
BciT130I CCWGG 2 cut(s) 390, 2907
BclI TGATCA 5 cut(s) 571, 796, 2017, 2347, 2515
BcnI CCSGG 2 cut(s) 231, 3426
BcoDI GTCTC 6 cut(s) 193, 626, 1337, 1381, 1725, 1941
BfaI CTAG 5 cut(s) 102, 836, 2676, 2730, 2997
BfmI CTRYAG 7 cut(s) 1533, 1752, 1779, 1951, 1995, 2403, 3339
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 3376
BglII AGATCT 2 cut(s) 13, 2399
BlpI GCTNAGC 1 cut(s) 2763
BmcAI AGTACT 1 cut(s) 2455
Bme1390I CCNGG 4 cut(s) 231, 390, 2907, 3426
Bme18I GGWCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
BmeT110I CYCGRG 3 cut(s) 94, 2615, 3294
BmgT120I GGNCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
BmiI GGNNCC 7 cut(s) 136, 418, 757, 1528, 1966, 2971, 3267
BmrFI CCNGG 4 cut(s) 231, 390, 2907, 3426
BmrI ACTGGG 4 cut(s) 2343, 3124, 3393, 3486
BmuI ACTGGG 4 cut(s) 2343, 3124, 3393, 3486
BpiI GAAGAC 2 cut(s) 530, 2613
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 875, 907
BpmI CTGGAG 4 cut(s) 1740, 1749, 1965, 2313
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 2763
Bpu14I TTCGAA 1 cut(s) 2723
BpuEI CTTGAG 2 cut(s) 2454, 3504
BpuMI CCSGG 2 cut(s) 231, 3426
BsaBI GATNNNNATC 2 cut(s) 216, 2346
BsaI GGTCTC 1 cut(s) 1725
BsaJI CCNNGG 7 cut(s) 229, 388, 2890, 2906, 3295, 3425, 3456
BsaWI WCCGGW 2 cut(s) 1414, 1897
BsaXI ACNNNNNCTCC 2 cut(s) 1356, 1386
Bsc4I CCNNNNNNNGG 7 cut(s) 372, 1361, 1414, 1421, 3299, 3300, 3301
Bse118I RCCGGY 1 cut(s) 2072
Bse3DI GCAATG 3 cut(s) 2803, 2949, 3450
Bse8I GATNNNNATC 2 cut(s) 216, 2346
BseAI TCCGGA 1 cut(s) 1897
BseBI CCWGG 2 cut(s) 390, 2907
BseDI CCNNGG 7 cut(s) 229, 388, 2890, 2906, 3295, 3425, 3456
BseGI GGATG 7 cut(s) 328, 388, 808, 2170, 2476, 2685, 3310
BseJI GATNNNNATC 2 cut(s) 216, 2346
BseLI CCNNNNNNNGG 7 cut(s) 372, 1361, 1414, 1421, 3299, 3300, 3301
BseMI GCAATG 3 cut(s) 2803, 2949, 3450
BsePI GCGCGC 2 cut(s) 112, 114
BseSI GKGCMC 2 cut(s) 42, 178
BseYI CCCAGC 1 cut(s) 2837
Bsh1236I CGCG 2 cut(s) 114, 116
BshFI GGCC 2 cut(s) 76, 3517
BshNI GGYRCC 3 cut(s) 416, 755, 3265
BsiHKAI GWGCWC 3 cut(s) 178, 2247, 3476
BsiHKCI CYCGRG 3 cut(s) 94, 2615, 3294
BsiSI CCGG 5 cut(s) 231, 1415, 1898, 2073, 3425
BslFI GGGAC 2 cut(s) 992, 1729
BslI CCNNNNNNNGG 7 cut(s) 372, 1361, 1414, 1421, 3299, 3300, 3301
BsmAI GTCTC 6 cut(s) 193, 626, 1337, 1381, 1725, 1941
BsmFI GGGAC 2 cut(s) 992, 1729
BsmI GAATGC 3 cut(s) 439, 1150, 3172
BsnI GGCC 2 cut(s) 76, 3517
Bso31I GGTCTC 1 cut(s) 1725
BsoBI CYCGRG 3 cut(s) 94, 2615, 3294
Bsp119I TTCGAA 1 cut(s) 2723
Bsp1286I GDGCHC 6 cut(s) 28, 42, 178, 1531, 2247, 3476
Bsp13I TCCGGA 1 cut(s) 1897
Bsp1407I TGTACA 1 cut(s) 1159
Bsp1720I GCTNAGC 1 cut(s) 2763
Bsp19I CCATGG 1 cut(s) 2890
BspANI GGCC 2 cut(s) 76, 3517
BspEI TCCGGA 1 cut(s) 1897
BspFNI CGCG 2 cut(s) 114, 116
BspHI TCATGA 3 cut(s) 2350, 2518, 3276
BspLI GGNNCC 7 cut(s) 136, 418, 757, 1528, 1966, 2971, 3267
BspMAI CTGCAG 3 cut(s) 1537, 1756, 3343
BspMI ACCTGC 1 cut(s) 3376
BspT104I TTCGAA 1 cut(s) 2723
BspT107I GGYRCC 3 cut(s) 416, 755, 3265
BspTNI GGTCTC 1 cut(s) 1725
BsrDI GCAATG 3 cut(s) 2803, 2949, 3450
BsrFI RCCGGY 1 cut(s) 2072
BsrGI TGTACA 1 cut(s) 1159
BssAI RCCGGY 1 cut(s) 2072
BssECI CCNNGG 7 cut(s) 229, 388, 2890, 2906, 3295, 3425, 3456
BssHII GCGCGC 2 cut(s) 112, 114
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 2 cut(s) 2890, 3456
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 2 cut(s) 390, 2907
Bst6I CTCTTC 4 cut(s) 1134, 1384, 2870, 3438
BstAUI TGTACA 1 cut(s) 1159
BstBI TTCGAA 1 cut(s) 2723
BstC8I GCNNGC 7 cut(s) 24, 114, 116, 160, 1405, 1433, 2794
BstDSI CCRYGG 1 cut(s) 2890
BstF5I GGATG 7 cut(s) 328, 388, 808, 2170, 2476, 2685, 3310
BstFNI CGCG 2 cut(s) 114, 116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 114, 116, 118, 164
BstMAI GTCTC 6 cut(s) 193, 626, 1337, 1381, 1725, 1941
BstMWI GCNNNNNNNGC 6 cut(s) 737, 1174, 1646, 1862, 2831, 3169
BstNI CCWGG 2 cut(s) 390, 2907
BstNSI RCATGY 3 cut(s) 1037, 1166, 1193
BstSCI CCNGG 4 cut(s) 229, 388, 2905, 3424
BstSFI CTRYAG 7 cut(s) 1533, 1752, 1779, 1951, 1995, 2403, 3339
BstSLI GKGCMC 2 cut(s) 42, 178
BstUI CGCG 2 cut(s) 114, 116
BstV2I GAAGAC 2 cut(s) 530, 2613
BstX2I RGATCY 7 cut(s) 13, 217, 290, 1417, 2203, 2399, 3119
BstXI CCANNNNNNTGG 4 cut(s) 455, 1676, 1892, 2440
BstYI RGATCY 7 cut(s) 13, 217, 290, 1417, 2203, 2399, 3119
BsuRI GGCC 2 cut(s) 76, 3517
BtgI CCRYGG 1 cut(s) 2890
BtsCI GGATG 7 cut(s) 328, 388, 808, 2170, 2476, 2685, 3310
BtsIMutI CAGTG 8 cut(s) 759, 1368, 1648, 1864, 2331, 2704, 3474, 3541
BveI ACCTGC 1 cut(s) 3376
Cac8I GCNNGC 7 cut(s) 24, 114, 116, 160, 1405, 1433, 2794
CaiI CAGNNNCTG 2 cut(s) 1541, 2207
CciI TCATGA 3 cut(s) 2350, 2518, 3276
CfoI GCGC 4 cut(s) 114, 116, 118, 164
Cfr10I RCCGGY 1 cut(s) 2072
Cfr13I GGNCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 1 cut(s) 3221
Csp6I GTAC 4 cut(s) 1160, 1805, 2454, 3344
CspCI CAANNNNNGTGG 2 cut(s) 3368, 3403
CspI CGGWCCG 1 cut(s) 233
CviQI GTAC 4 cut(s) 1160, 1805, 2454, 3344
DraI TTTAAA 1 cut(s) 1501
DraIII CACNNNGTG 1 cut(s) 55
DrdI GACNNNNNNGTC 2 cut(s) 1723, 2617
DseDI GACNNNNNNGTC 2 cut(s) 1723, 2617
Eam1104I CTCTTC 4 cut(s) 1134, 1384, 2870, 3438
EarI CTCTTC 4 cut(s) 1134, 1384, 2870, 3438
Ecl136II GAGCTC 1 cut(s) 2245
Eco130I CCWWGG 2 cut(s) 2890, 3456
Eco147I AGGCCT 1 cut(s) 3517
Eco24I GRGCYC 3 cut(s) 28, 1531, 2247
Eco31I GGTCTC 1 cut(s) 1725
Eco32I GATATC 1 cut(s) 2329
Eco47I GGWCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
Eco53kI GAGCTC 1 cut(s) 2245
Eco57I CTGAAG 6 cut(s) 603, 681, 828, 1605, 1821, 2979
Eco88I CYCGRG 3 cut(s) 94, 2615, 3294
EcoICRI GAGCTC 1 cut(s) 2245
EcoRI GAATTC 1 cut(s) 2725
EcoRII CCWGG 2 cut(s) 388, 2905
EcoRV GATATC 1 cut(s) 2329
EcoT14I CCWWGG 2 cut(s) 2890, 3456
EcoT22I ATGCAT 5 cut(s) 511, 733, 990, 1392, 2374
EcoT38I GRGCYC 3 cut(s) 28, 1531, 2247
ErhI CCWWGG 2 cut(s) 2890, 3456
FalI AAGNNNNNCTT 6 cut(s) 1392, 1424, 1749, 1781, 1965, 1997
FaqI GGGAC 2 cut(s) 992, 1729
FauI CCCGC 3 cut(s) 62, 1975, 2476
FbaI TGATCA 5 cut(s) 571, 796, 2017, 2347, 2515
FokI GGATG 7 cut(s) 335, 395, 815, 2177, 2463, 2692, 3317
FriOI GRGCYC 3 cut(s) 28, 1531, 2247
FspBI CTAG 5 cut(s) 102, 836, 2676, 2730, 2997
GlaI GCGC 4 cut(s) 113, 115, 117, 163
GsaI CCCAGC 1 cut(s) 2841
GsuI CTGGAG 4 cut(s) 1740, 1749, 1965, 2313
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 76, 3517
HapII CCGG 5 cut(s) 231, 1415, 1898, 2073, 3425
HgaI GACGC 1 cut(s) 3221
HhaI GCGC 4 cut(s) 114, 116, 118, 164
Hin6I GCGC 4 cut(s) 112, 114, 116, 162
HinP1I GCGC 4 cut(s) 112, 114, 116, 162
HincII GTYRAC 1 cut(s) 2050
HindII GTYRAC 1 cut(s) 2050
HpaI GTTAAC 1 cut(s) 2050
HpaII CCGG 5 cut(s) 231, 1415, 1898, 2073, 3425
Hpy166II GTNNAC 6 cut(s) 176, 698, 1580, 1630, 1846, 2050
Hpy8I GTNNAC 6 cut(s) 176, 698, 1580, 1630, 1846, 2050
HpyF10VI GCNNNNNNNGC 6 cut(s) 737, 1174, 1646, 1862, 2831, 3169
HspAI GCGC 4 cut(s) 112, 114, 116, 162
Kpn2I TCCGGA 1 cut(s) 1897
Ksp22I TGATCA 5 cut(s) 571, 796, 2017, 2347, 2515
KspAI GTTAAC 1 cut(s) 2050
LmnI GCTCC 7 cut(s) 252, 964, 1526, 2250, 2683, 2975, 3471
MaeI CTAG 5 cut(s) 102, 836, 2676, 2730, 2997
MfeI CAATTG 1 cut(s) 3069
MflI RGATCY 7 cut(s) 13, 217, 290, 1417, 2203, 2399, 3119
MhlI GDGCHC 6 cut(s) 28, 42, 178, 1531, 2247, 3476
MmeI TCCRAC 2 cut(s) 2487, 3025
Mph1103I ATGCAT 5 cut(s) 511, 733, 990, 1392, 2374
MroI TCCGGA 1 cut(s) 1897
MroXI GAANNNNTTC 2 cut(s) 948, 2589
MseI TTAA 8 cut(s) 297, 560, 708, 744, 1443, 1500, 2049, 2253
MslI CAYNNNNRTG 6 cut(s) 96, 210, 453, 972, 1190, 2277
MspA1I CMGCKG 2 cut(s) 2015, 2483
MspI CCGG 5 cut(s) 231, 1415, 1898, 2073, 3425
MspR9I CCNGG 4 cut(s) 231, 390, 2907, 3426
MunI CAATTG 1 cut(s) 3069
Mva1269I GAATGC 3 cut(s) 439, 1150, 3172
MvaI CCWGG 2 cut(s) 390, 2907
MvnI CGCG 2 cut(s) 114, 116
MwoI GCNNNNNNNGC 6 cut(s) 737, 1174, 1646, 1862, 2831, 3169
NciI CCSGG 2 cut(s) 231, 3426
NcoI CCATGG 1 cut(s) 2890
NlaIV GGNNCC 7 cut(s) 136, 418, 757, 1528, 1966, 2971, 3267
NmeAIII GCCGAG 1 cut(s) 1401
NsiI ATGCAT 5 cut(s) 511, 733, 990, 1392, 2374
NspI RCATGY 3 cut(s) 1037, 1166, 1193
NspV TTCGAA 1 cut(s) 2723
OliI CACNNNNGTG 1 cut(s) 96
PagI TCATGA 3 cut(s) 2350, 2518, 3276
PauI GCGCGC 2 cut(s) 112, 114
PceI AGGCCT 1 cut(s) 3517
PciI ACATGT 3 cut(s) 1033, 1162, 1189
PctI GAATGC 3 cut(s) 439, 1150, 3172
PdmI GAANNNNTTC 2 cut(s) 948, 2589
PfeI GAWTC 9 cut(s) 85, 665, 1022, 1595, 1940, 2222, 2444, 2864, 2930
PscI ACATGT 3 cut(s) 1033, 1162, 1189
Psp124BI GAGCTC 1 cut(s) 2247
Psp6I CCWGG 2 cut(s) 388, 2905
PspFI CCCAGC 1 cut(s) 2837
PspGI CCWGG 2 cut(s) 388, 2905
PspN4I GGNNCC 7 cut(s) 136, 418, 757, 1528, 1966, 2971, 3267
PspPI GGNCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
PstI CTGCAG 3 cut(s) 1537, 1756, 3343
PstNI CAGNNNCTG 2 cut(s) 1541, 2207
PsuI RGATCY 7 cut(s) 13, 217, 290, 1417, 2203, 2399, 3119
PteI GCGCGC 2 cut(s) 112, 114
PvuII CAGCTG 1 cut(s) 2015
RsaI GTAC 4 cut(s) 1161, 1806, 2455, 3345
RsaNI GTAC 4 cut(s) 1160, 1805, 2454, 3344
RseI CAYNNNNRTG 6 cut(s) 96, 210, 453, 972, 1190, 2277
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 2247
SaqAI TTAA 8 cut(s) 297, 560, 708, 744, 1443, 1500, 2049, 2253
Sau96I GGNCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
ScaI AGTACT 1 cut(s) 2455
ScrFI CCNGG 4 cut(s) 231, 390, 2907, 3426
SduI GDGCHC 6 cut(s) 28, 42, 178, 1531, 2247, 3476
SfcI CTRYAG 7 cut(s) 1533, 1752, 1779, 1951, 1995, 2403, 3339
SfuI TTCGAA 1 cut(s) 2723
SinI GGWCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
SmiMI CAYNNNNRTG 6 cut(s) 96, 210, 453, 972, 1190, 2277
SmlI CTYRAG 2 cut(s) 2469, 3519
SmoI CTYRAG 2 cut(s) 2469, 3519
SseBI AGGCCT 1 cut(s) 3517
SspI AATATT 1 cut(s) 2590
SspMI CTAG 5 cut(s) 102, 836, 2676, 2730, 2997
SstI GAGCTC 1 cut(s) 2247
StuI AGGCCT 1 cut(s) 3517
StyD4I CCNGG 4 cut(s) 229, 388, 2905, 3424
StyI CCWWGG 2 cut(s) 2890, 3456
TaqI TCGA 1 cut(s) 2723
TaqII GACCGA 1 cut(s) 542
TatI WGTACW 3 cut(s) 1159, 2453, 3343
TauI GCSGC 1 cut(s) 2970
TfiI GAWTC 9 cut(s) 85, 665, 1022, 1595, 1940, 2222, 2444, 2864, 2930
Tru1I TTAA 8 cut(s) 297, 560, 708, 744, 1443, 1500, 2049, 2253
Tru9I TTAA 8 cut(s) 297, 560, 708, 744, 1443, 1500, 2049, 2253
TscAI CASTG 7 cut(s) 766, 1375, 1648, 1864, 2338, 2704, 3481
TspGWI ACGGA 3 cut(s) 432, 1822, 1977
TspRI CASTG 7 cut(s) 766, 1375, 1648, 1864, 2338, 2704, 3481
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 7 cut(s) 233, 363, 1679, 1895, 1964, 2887, 2980
XapI RAATTY 6 cut(s) 2034, 2191, 2540, 2725, 2748, 3218
XbaI TCTAGA 1 cut(s) 835
XceI RCATGY 3 cut(s) 1037, 1166, 1193
XmnI GAANNNNTTC 2 cut(s) 948, 2589
XspI CTAG 5 cut(s) 102, 836, 2676, 2730, 2997
ZrmI AGTACT 1 cut(s) 2455
Zsp2I ATGCAT 5 cut(s) 511, 733, 990, 1392, 2374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.