Prupe.1G170500_v2.0.a1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001352
Physical Location & Seq
Forward (+)
14049502 .. 14056716
7215 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G170500.2

Sequence Viewer

Length: 3147 bp
ATGGATAGCCAAGATCTAGCAAGAACTCACAGTTCAGGGCTTGACAGCCATGGAGTCCATGTTTGTCACAAATGTGGGTGGCCCTTTCCAAACGCACACCCAAGTGCCAGACACAGACGTGCCCATAAGAGGATTTGTGGAACCATTGATGGTTACAAGCTGGCTGGCTCTGAGAAGAACACCCATGTAAATGATTCTGATGATGAGCATTCTGATGAAGAACGCAAAACCCCCAATCTAGTATCCGTGAGATCAGAAGACGTGGTGTTCTCAGATGCCGTTGCAGAATTTTCAGATAGTGCTTCTGGTGCAGGAACCGGTGAAGGGTTAGAAAATGTTCGAGAATCAGAAATTAATCTGGAAAGGGTTGCCAAGAATGGTTTGAACGTCATCCAATCCCTTAATGATGGGGAAATCACCGAGAATACTACTAATCAGCTGGGTGCACAAGAGTTACAGGGATCGTTGTCAACTTCTAACATCATTTCAATGACGAGTTCCGAATCAGCTTTGCAGGATGATAGTAATCATTCAGAAGATGACGTACAACCCATTCAATTTGAAGTGCTGGCAGATGCATCAGAAGATAGTAAGAAGACCCATGCTACTGACAGTGTGATTGAGAGCTCTTTATTATCTGCTCCACAAGAGGACAAGATTGATGGAATTACTACAAGTAATCTAGATGGAGACTTACCGTGCACTGTGATCTCACCCAATAAAGTTGTCAGTGAAACAGCTGAGGGTGTATCTAAGTTAGAAGGGACAGTTGAAATGACTTCGGACTCTCTGCGAGATAATGAAGTTGTTCCATCAGAGAAAGAGTACACCCATGCATATGATTTGTATATTCCTCAAATTGATCCTTCCCCTGAAGTTGAATCTGTTGAACATGTGAATGCATCGGTTGATACTGTAGAGAATAAGGTGGATACTACAGACAGAATACACTTTTCAAATTCTGGTGGGTTAACTGAGAGTAGCAACTGGGAGGGAGAAGGGAATGCAAATGTACATGTGCTTTCTGTTCCAGATGATACACATGTTGTAGAAAATCCTGAGGCGATGCTTGAAGGTTTTAAAAACCACAAAGAAAAGAAATTAGACCAGCCTATCTTCTTGGACTCTTGGGAACAATTTAATGATGAAGAAAATGATGTTAAACGTGGAGGTAGCAGCAAGCCAATGGTTAAAGAAGTGCCTGCTGAAGAAGAACCCAGTGTGTCTCACATCCAGATCAAGAGTACTGAAAACCAAAGGTCAGATGAAATAGCAGCCCCTGCAGATGCAAATGAATCATTACAGGTGAACTTTTCTGAAAGTGATACAAATTCCTTGATTGCTTCCAGCAGTGCTGCTATAAATCAAACAAGGCACTTAGATGATCTGGATGGTAATGGAAAAGATGAAAAATGTGATTTAAGCGCAGACCATAGCAGCAAAGGACCTGCAGAAGAAAATTCTGTGAAAAATCCTGCTGAAATCGAATTTAATCCAGCAGCTAATTTACCTGAAAAGGGGGATGCTGGTGATTATGAGAAGGGTATATTTGAAAACTGCAATATAATTGGCGATTCTGGTTATCATGAAACTGAAAATTTTAAGTTAGCTGGGGATGACAACAGCAAGGAATCTACAGAGGATTTTTTTGCTAAAGACCCTGTGAGTAATTTGGAATCTGCTAGTCGTCTCTCTGAGCTCCAAGTTTCTCCAGTATCCAGCTCGCATGAAGTTGGTGATGCAGGTGATTATGAGAAGGATAAAACTGAAAGGTTTGATATAATTGGGGATGATGGTGATCATGAGAAAGCTGAAAAGTATGTTTTAACTGGGGATGACTACAGGAAAGTATCTACAGAGAAAATTGTTGCAGAGAATTCTATAACTAGTTTGGAATCTGCCAGTAGTCTCTCCGAACTTCAAGCTAATTTAGCAACAAATTTAAATGATGATGACCCTGGCGATCATGATGGTGGTGAAATTGAAAAGAGTATTATATCTGGAAATGAGAGCAGTGAAGGACCTGTTGAAAAAAATCTTTTGGTGAAGCCAACATTGAACCTTGATTCTGCTATTGATCTTGTTGAATCAAAAACTGTTGCAGAAGATGCAGTGGATGGTTCTATGCTGAAGCTGGTTGACACCAGACACAAAACTTTGAATAGCAGTTCAAATTCTAGCGGAGTTGGCCTCAATATAGTTTCAGATCATGGTCCCAATGCTGAGTCCTCACAGAAGAGTTCAGAGGGTTGTGGGATGAAAGAACTAGGTCTTTCTGCTTTAGATGCTGAATCTTTTGTTCCGAGTTTCACTGCTGAAGATATTGGCTCCAGAGAGTTCCATGGGGTATCTTCGGGGTTGAGTTCTCAATCTTTTCAAGAAGAGAGTGATAAAAACTTTGTTAAGCAACAGCTAAGTTCACCTGCACTGGATGTTGTTGACTCAAATAGCCAAACTGACAGTCTGGAAGGAAACTGGGGCTCTGTTTCAGTTCTTTCTATCCAGTCAGATGCACAAGCTGTACCACCTACAGATTCCCAAACTTCAGTGGAAGAGAAGAAGTCAAGAGCTGCATCTGAGAGACAACACCCTGAAAAATCTGATATGTTTGAGGCACCGTCTTTCATGACATTGGTTGAACCTAGAGGCGTGAATGACCAAAAAGCTACTGCTGCTGAAATTCACACAGCACATAACCCAGAGCAGCCAAAACCTGCACCTTTGCAGGCAGGGTGGTTTCCTTCTATCAGTCATGTTGTGAACGAATCACCGGGGAGAAAGAAAAACGAAGAGATCATTGCCAAGGTTACAAACTGGAGCACTGGAAAGCAACACACCCCTTTGAAGAACCTTTTGGGTGAGGCCTATCTAGAAAATAAAGCAAAGTCACCGACCCAAAAAGAAAGTCAAGCTCCTGCGCCTCAGAGGGATGATAAAGCAGTGAAGGTGAAGGATGGTGGTCCTGGAGCGACAACAGTGAACTCAATTCTGGGTCCTGAATCACCAACAGGTCAGGCATCTAAGAAAGAGAATGCAAAAGAATGGAACTCTCCGGCACGGTATCCTTCAGACATTAAGAGTGAAAAGAAGAAAGTCAAGGGTAGACCATATTGGGCGCAATTTGTATGCTGCTCATCCGTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1049

Amino Acids

113.16

Weight (kDa)

4.59

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1733, 2431
Acc36I ACCTGC 4 cut(s) 1456, 1733, 2431, 2722
AccB1I GGYRCC 1 cut(s) 2614
AccI GTMKAC 1 cut(s) 3103
AciI CCGC 1 cut(s) 2181
AclWI GGATC 2 cut(s) 469, 857
AcuI CTGAAG 6 cut(s) 894, 1227, 2150, 2337, 2529, 3051
AfaI GTAC 5 cut(s) 546, 827, 1014, 1246, 2523
AfiI CCNNNNNNNGG 3 cut(s) 129, 324, 1517
AflIII ACRYGT 3 cut(s) 892, 1015, 1042
AgeI ACCGGT 1 cut(s) 317
AhlI ACTAGT 1 cut(s) 1886
AjiI CACGTC 2 cut(s) 119, 262
AjnI CCWGG 2 cut(s) 1957, 2962
AjuI GAANNNNNNNTTGG 2 cut(s) 2837, 2869
AleI CACNNNNGTG 3 cut(s) 72, 102, 117
Alw21I GWGCWC 5 cut(s) 448, 629, 704, 1701, 2822
Alw26I GTCTC 5 cut(s) 684, 1230, 1694, 1913, 2575
Alw44I GTGCAC 2 cut(s) 444, 700
AlwI GGATC 2 cut(s) 469, 857
AlwNI CAGNNNCTG 1 cut(s) 1280
AoxI GGCC 3 cut(s) 80, 2188, 2862
ApaLI GTGCAC 2 cut(s) 444, 700
ApeKI GCWGC 9 cut(s) 1176, 1274, 1355, 1437, 1499, 2570, 2672, 2704, 3129
ArsI GACNNNNNNTTYG 4 cut(s) 2446, 2478, 2890, 2922
AseI ATTAAT 1 cut(s) 354
AsiGI ACCGGT 1 cut(s) 317
Asp700I GAANNNNTTC 2 cut(s) 336, 807
AspLEI GCGC 3 cut(s) 1427, 2920, 3118
AspS9I GGNCC 6 cut(s) 81, 1445, 2021, 2213, 2960, 2993
AsuC2I CCSGG 1 cut(s) 2772
AvaII GGWCC 5 cut(s) 1445, 2021, 2213, 2960, 2993
AxyI CCTNAGG 1 cut(s) 1059
BaeGI GKGCMC 3 cut(s) 124, 448, 704
BaeI ACNNNNGTAYC 2 cut(s) 1029, 1062
BanI GGYRCC 1 cut(s) 2614
BanII GRGCYC 3 cut(s) 629, 1701, 2483
BarI GAAGNNNNNNTAC 2 cut(s) 528, 560
BbsI GAAGAC 2 cut(s) 264, 602
Bbv12I GWGCWC 5 cut(s) 448, 629, 704, 1701, 2822
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 9 cut(s) 1188, 1286, 1342, 1449, 1511, 2557, 2659, 2716, 3116
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 2 cut(s) 1959, 2964
BciVI GTATCC 4 cut(s) 253, 925, 1726, 3072
BclI TGATCA 1 cut(s) 1798
BcnI CCSGG 1 cut(s) 2772
BcoDI GTCTC 5 cut(s) 684, 1230, 1694, 1913, 2575
BcuI ACTAGT 1 cut(s) 1886
BfaI CTAG 9 cut(s) 17, 239, 683, 1683, 1887, 2178, 2267, 2643, 2870
BfmI CTRYAG 8 cut(s) 915, 936, 1281, 1449, 1635, 1840, 1854, 2529
BfuAI ACCTGC 4 cut(s) 1456, 1733, 2431, 2722
BfuI GTATCC 4 cut(s) 253, 925, 1726, 3072
BglII AGATCT 1 cut(s) 13
BisI GCNGC 9 cut(s) 1177, 1275, 1356, 1438, 1500, 2571, 2673, 2705, 3130
BlsI GCNGC 9 cut(s) 1178, 1276, 1357, 1439, 1501, 2572, 2674, 2706, 3131
BmcAI AGTACT 1 cut(s) 1246
Bme1390I CCNGG 3 cut(s) 1959, 2772, 2964
Bme18I GGWCC 5 cut(s) 1445, 2021, 2213, 2960, 2993
BmgBI CACGTC 2 cut(s) 119, 262
BmgT120I GGNCC 6 cut(s) 81, 1445, 2021, 2213, 2960, 2993
BmiI GGNNCC 6 cut(s) 142, 316, 2215, 2329, 2616, 2994
BmrFI CCNGG 3 cut(s) 1959, 2772, 2964
BmrI ACTGGG 4 cut(s) 997, 1212, 1839, 2485
BmuI ACTGGG 4 cut(s) 997, 1212, 1839, 2485
BpiI GAAGAC 2 cut(s) 264, 602
BplI GAGNNNNNCTC 2 cut(s) 2175, 2207
BpmI CTGGAG 4 cut(s) 1695, 2314, 2836, 2985
Bpu10I CCTNAGC 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 2772
BsaBI GATNNNNATC 2 cut(s) 525, 1797
BsaJI CCNNGG 5 cut(s) 49, 1957, 2341, 2771, 2802
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 324, 1517
Bse118I RCCGGY 1 cut(s) 317
Bse21I CCTNAGG 1 cut(s) 1059
Bse3DI GCAATG 1 cut(s) 2796
Bse8I GATNNNNATC 2 cut(s) 525, 1797
BseBI CCWGG 2 cut(s) 1959, 2964
BseDI CCNNGG 5 cut(s) 49, 1957, 2341, 2771, 2802
BseJI GATNNNNATC 2 cut(s) 525, 1797
BseLI CCNNNNNNNGG 3 cut(s) 129, 324, 1517
BseMI GCAATG 1 cut(s) 2796
BseMII CTCAG 9 cut(s) 162, 285, 732, 966, 1050, 1686, 2214, 2568, 2936
BseSI GKGCMC 3 cut(s) 124, 448, 704
BseXI GCAGC 9 cut(s) 1188, 1286, 1342, 1449, 1511, 2557, 2659, 2716, 3116
BseYI CCCAGC 2 cut(s) 439, 1610
BsgI GTGCAG 3 cut(s) 330, 2409, 2700
BshFI GGCC 3 cut(s) 82, 2190, 2864
BshNI GGYRCC 1 cut(s) 2614
BshTI ACCGGT 1 cut(s) 317
BsiHKAI GWGCWC 5 cut(s) 448, 629, 704, 1701, 2822
BsiSI CCGG 3 cut(s) 318, 2771, 3053
BslFI GGGAC 2 cut(s) 778, 2199
BslI CCNNNNNNNGG 3 cut(s) 129, 324, 1517
BsmAI GTCTC 5 cut(s) 684, 1230, 1694, 1913, 2575
BsmBI CGTCTC 1 cut(s) 1694
BsmFI GGGAC 2 cut(s) 778, 2199
BsmI GAATGC 4 cut(s) 208, 904, 1009, 3037
BsnI GGCC 3 cut(s) 82, 2190, 2864
Bsp1286I GDGCHC 7 cut(s) 124, 448, 629, 704, 1701, 2483, 2822
Bsp1407I TGTACA 1 cut(s) 1012
Bsp19I CCATGG 2 cut(s) 49, 2341
BspACI CCGC 1 cut(s) 2181
BspANI GGCC 3 cut(s) 82, 2190, 2864
BspCNI CTCAG 9 cut(s) 163, 284, 733, 967, 1051, 1687, 2215, 2569, 2935
BspHI TCATGA 4 cut(s) 1585, 1801, 1966, 2625
BspLI GGNNCC 6 cut(s) 142, 316, 2215, 2329, 2616, 2994
BspMAI CTGCAG 2 cut(s) 1285, 1453
BspMI ACCTGC 4 cut(s) 1456, 1733, 2431, 2722
BspPI GGATC 2 cut(s) 469, 857
BspT107I GGYRCC 1 cut(s) 2614
BsrDI GCAATG 1 cut(s) 2796
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 1012
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 5 cut(s) 49, 1957, 2341, 2771, 2802
BssT1I CCWWGG 3 cut(s) 49, 2341, 2802
Bst2UI CCWGG 2 cut(s) 1959, 2964
Bst6I CTCTTC 4 cut(s) 2231, 2376, 2547, 2784
BstAPI GCANNNNNTGC 2 cut(s) 1280, 2108
BstAUI TGTACA 1 cut(s) 1012
BstC8I GCNNGC 7 cut(s) 162, 166, 570, 1181, 1203, 1724, 2727
BstDSI CCRYGG 2 cut(s) 49, 2341
BstHHI GCGC 3 cut(s) 1427, 2920, 3118
BstMAI GTCTC 5 cut(s) 684, 1230, 1694, 1913, 2575
BstMWI GCNNNNNNNGC 7 cut(s) 308, 1280, 1931, 2108, 2187, 2285, 2672
BstNI CCWGG 2 cut(s) 1959, 2964
BstNSI RCATGY 3 cut(s) 896, 1019, 1046
BstSCI CCNGG 3 cut(s) 1957, 2770, 2962
BstSFI CTRYAG 8 cut(s) 915, 936, 1281, 1449, 1635, 1840, 1854, 2529
BstSLI GKGCMC 3 cut(s) 124, 448, 704
BstV1I GCAGC 9 cut(s) 1188, 1286, 1342, 1449, 1511, 2557, 2659, 2716, 3116
BstV2I GAAGAC 2 cut(s) 264, 602
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
Bsu36I CCTNAGG 1 cut(s) 1059
BsuI GTATCC 4 cut(s) 253, 925, 1726, 3072
BsuRI GGCC 3 cut(s) 82, 2190, 2864
BtgI CCRYGG 2 cut(s) 49, 2341
BtgZI GCGATG 1 cut(s) 1079
BtrI CACGTC 2 cut(s) 119, 262
BtsI GCAGTG 5 cut(s) 1357, 2020, 2118, 2310, 2946
BveI ACCTGC 4 cut(s) 1456, 1733, 2431, 2722
Cac8I GCNNGC 7 cut(s) 162, 166, 570, 1181, 1203, 1724, 2727
CaiI CAGNNNCTG 1 cut(s) 1280
CciI TCATGA 4 cut(s) 1585, 1801, 1966, 2625
CfoI GCGC 3 cut(s) 1427, 2920, 3118
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 6 cut(s) 81, 1445, 2021, 2213, 2960, 2993
Csp6I GTAC 5 cut(s) 545, 826, 1013, 1245, 2522
CspAI ACCGGT 1 cut(s) 317
CviQI GTAC 5 cut(s) 545, 826, 1013, 1245, 2522
DraI TTTAAA 2 cut(s) 1081, 1944
Eam1104I CTCTTC 4 cut(s) 2231, 2376, 2547, 2784
EarI CTCTTC 4 cut(s) 2231, 2376, 2547, 2784
Ecl136II GAGCTC 2 cut(s) 627, 1699
Eco130I CCWWGG 3 cut(s) 49, 2341, 2802
Eco147I AGGCCT 1 cut(s) 2864
Eco24I GRGCYC 3 cut(s) 629, 1701, 2483
Eco47I GGWCC 5 cut(s) 1445, 2021, 2213, 2960, 2993
Eco53kI GAGCTC 2 cut(s) 627, 1699
Eco57I CTGAAG 6 cut(s) 894, 1227, 2150, 2337, 2529, 3051
Eco81I CCTNAGG 1 cut(s) 1059
EcoICRI GAGCTC 2 cut(s) 627, 1699
EcoO109I RGGNCCY 3 cut(s) 1445, 2021, 2993
EcoRI GAATTC 1 cut(s) 1876
EcoRII CCWGG 2 cut(s) 1957, 2962
EcoT14I CCWWGG 3 cut(s) 49, 2341, 2802
EcoT22I ATGCAT 3 cut(s) 580, 838, 904
EcoT38I GRGCYC 3 cut(s) 629, 1701, 2483
ErhI CCWWGG 3 cut(s) 49, 2341, 2802
Esp3I CGTCTC 1 cut(s) 1694
FaqI GGGAC 2 cut(s) 778, 2199
FauNDI CATATG 1 cut(s) 838
FbaI TGATCA 1 cut(s) 1798
FblI GTMKAC 1 cut(s) 3103
Fnu4HI GCNGC 9 cut(s) 1177, 1275, 1356, 1438, 1500, 2571, 2673, 2705, 3130
FriOI GRGCYC 3 cut(s) 629, 1701, 2483
Fsp4HI GCNGC 9 cut(s) 1177, 1275, 1356, 1438, 1500, 2571, 2673, 2705, 3130
FspBI CTAG 9 cut(s) 17, 239, 683, 1683, 1887, 2178, 2267, 2643, 2870
GlaI GCGC 3 cut(s) 1426, 2919, 3117
GluI GCNGC 9 cut(s) 1177, 1275, 1356, 1438, 1500, 2571, 2673, 2705, 3130
GsaI CCCAGC 2 cut(s) 443, 1614
GsuI CTGGAG 4 cut(s) 1695, 2314, 2836, 2985
HaeIII GGCC 3 cut(s) 82, 2190, 2864
HapII CCGG 3 cut(s) 318, 2771, 3053
HhaI GCGC 3 cut(s) 1427, 2920, 3118
Hin6I GCGC 3 cut(s) 1425, 2918, 3116
HinP1I GCGC 3 cut(s) 1425, 2918, 3116
HincII GTYRAC 4 cut(s) 471, 972, 2140, 2440
HindII GTYRAC 4 cut(s) 471, 972, 2140, 2440
HpaI GTTAAC 1 cut(s) 972
HpaII CCGG 3 cut(s) 318, 2771, 3053
HpyCH4IV ACGT 5 cut(s) 118, 261, 387, 543, 1165
HpyF10VI GCNNNNNNNGC 7 cut(s) 308, 1280, 1931, 2108, 2187, 2285, 2672
HpySE526I ACGT 5 cut(s) 118, 261, 387, 543, 1165
HspAI GCGC 3 cut(s) 1425, 2918, 3116
Ksp22I TGATCA 1 cut(s) 1798
KspAI GTTAAC 1 cut(s) 972
LmnI GCTCC 6 cut(s) 646, 1704, 2333, 2817, 2917, 2966
Lsp1109I GCAGC 9 cut(s) 1188, 1286, 1342, 1449, 1511, 2557, 2659, 2716, 3116
MaeI CTAG 9 cut(s) 17, 239, 683, 1683, 1887, 2178, 2267, 2643, 2870
MaeII ACGT 5 cut(s) 118, 261, 387, 543, 1165
MaeIII GTNAC 5 cut(s) 65, 152, 453, 2806, 2886
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 7 cut(s) 124, 448, 629, 704, 1701, 2483, 2822
MlyI GAGTC 5 cut(s) 63, 779, 1118, 2234, 2435
Mph1103I ATGCAT 3 cut(s) 580, 838, 904
MroXI GAANNNNTTC 2 cut(s) 336, 807
MspA1I CMGCKG 2 cut(s) 439, 740
MspI CCGG 3 cut(s) 318, 2771, 3053
MspR9I CCNGG 3 cut(s) 1959, 2772, 2964
Mva1269I GAATGC 4 cut(s) 208, 904, 1009, 3037
MvaI CCWGG 2 cut(s) 1959, 2964
MwoI GCNNNNNNNGC 7 cut(s) 308, 1280, 1931, 2108, 2187, 2285, 2672
NciI CCSGG 1 cut(s) 2772
NcoI CCATGG 2 cut(s) 49, 2341
NdeI CATATG 1 cut(s) 838
NlaIV GGNNCC 6 cut(s) 142, 316, 2215, 2329, 2616, 2994
NmuCI GTSAC 2 cut(s) 65, 2886
NsiI ATGCAT 3 cut(s) 580, 838, 904
NspI RCATGY 3 cut(s) 896, 1019, 1046
OliI CACNNNNGTG 3 cut(s) 72, 102, 117
PagI TCATGA 4 cut(s) 1585, 1801, 1966, 2625
PaqCI CACCTGC 2 cut(s) 1733, 2431
PceI AGGCCT 1 cut(s) 2864
PciI ACATGT 3 cut(s) 892, 1015, 1042
PctI GAATGC 4 cut(s) 208, 904, 1009, 3037
PdmI GAANNNNTTC 2 cut(s) 336, 807
PfoI TCCNGGA 1 cut(s) 2962
PinAI ACCGGT 1 cut(s) 317
PkrI GCNGC 9 cut(s) 1178, 1276, 1357, 1439, 1501, 2572, 2674, 2706, 3131
PleI GAGTC 5 cut(s) 62, 779, 1118, 2233, 2435
PpsI GAGTC 5 cut(s) 62, 779, 1118, 2233, 2435
PpuMI RGGWCCY 3 cut(s) 1445, 2021, 2993
PscI ACATGT 3 cut(s) 892, 1015, 1042
PshBI ATTAAT 1 cut(s) 354
Psp124BI GAGCTC 2 cut(s) 629, 1701
Psp5II RGGWCCY 3 cut(s) 1445, 2021, 2993
Psp6I CCWGG 2 cut(s) 1957, 2962
PspFI CCCAGC 2 cut(s) 439, 1610
PspGI CCWGG 2 cut(s) 1957, 2962
PspN4I GGNNCC 6 cut(s) 142, 316, 2215, 2329, 2616, 2994
PspPI GGNCC 6 cut(s) 81, 1445, 2021, 2213, 2960, 2993
PspPPI RGGWCCY 3 cut(s) 1445, 2021, 2993
PstI CTGCAG 2 cut(s) 1285, 1453
PstNI CAGNNNCTG 1 cut(s) 1280
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 2 cut(s) 439, 740
RsaI GTAC 5 cut(s) 546, 827, 1014, 1246, 2523
RsaNI GTAC 5 cut(s) 545, 826, 1013, 1245, 2522
SacI GAGCTC 2 cut(s) 629, 1701
SatI GCNGC 9 cut(s) 1177, 1275, 1356, 1438, 1500, 2571, 2673, 2705, 3130
Sau96I GGNCC 6 cut(s) 81, 1445, 2021, 2213, 2960, 2993
ScaI AGTACT 1 cut(s) 1246
SchI GAGTC 5 cut(s) 63, 779, 1118, 2234, 2435
ScrFI CCNGG 3 cut(s) 1959, 2772, 2964
SduI GDGCHC 7 cut(s) 124, 448, 629, 704, 1701, 2483, 2822
SfcI CTRYAG 8 cut(s) 915, 936, 1281, 1449, 1635, 1840, 1854, 2529
SinI GGWCC 5 cut(s) 1445, 2021, 2213, 2960, 2993
SmiI ATTTAAAT 1 cut(s) 1944
SpeI ACTAGT 1 cut(s) 1886
SseBI AGGCCT 1 cut(s) 2864
SsiI CCGC 1 cut(s) 2181
SspMI CTAG 9 cut(s) 17, 239, 683, 1683, 1887, 2178, 2267, 2643, 2870
SstI GAGCTC 2 cut(s) 629, 1701
StuI AGGCCT 1 cut(s) 2864
StyD4I CCNGG 3 cut(s) 1957, 2770, 2962
StyI CCWWGG 3 cut(s) 49, 2341, 2802
SwaI ATTTAAAT 1 cut(s) 1944
TaiI ACGT 5 cut(s) 121, 264, 390, 546, 1168
TaqI TCGA 2 cut(s) 340, 1485
TatI WGTACW 3 cut(s) 825, 1012, 1244
TseFI GTSAC 2 cut(s) 65, 2886
TseI GCWGC 9 cut(s) 1176, 1274, 1355, 1437, 1499, 2570, 2672, 2704, 3129
Tsp45I GTSAC 2 cut(s) 65, 2886
TspGWI ACGGA 2 cut(s) 235, 3127
VneI GTGCAC 2 cut(s) 444, 700
VpaK11BI GGWCC 5 cut(s) 1445, 2021, 2213, 2960, 2993
VspI ATTAAT 1 cut(s) 354
XbaI TCTAGA 2 cut(s) 682, 2869
XceI RCATGY 3 cut(s) 896, 1019, 1046
XmiI GTMKAC 1 cut(s) 3103
XmnI GAANNNNTTC 2 cut(s) 336, 807
XspI CTAG 9 cut(s) 17, 239, 683, 1683, 1887, 2178, 2267, 2643, 2870
ZrmI AGTACT 1 cut(s) 1246
Zsp2I ATGCAT 3 cut(s) 580, 838, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.