Rh2AG342700

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001352
Physical Location & Seq
Forward (+)
50168861 .. 50169283
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG342700.1

Sequence Viewer

Length: 423 bp
ATGCATCATCTAGAGACATTAGTAAAAGCTCTACAGAAAAAACCTTTGCGGAGGACTCTACTAAAGAAGAACGAAGAGACCATTGCTAAGGTGACAAACGGGAGCACTGGGAAGCAACACACTCCTTTGAAAAGCCTTTTGGGGGAGGCCTCAAGTGAAAGCAAAGGAAAGCCACTGAACCAAAAAGAAAATCTTGCTCCTGCTCCTGAGAAAGATGATAAAGCTGCAAAGGTCAGTGGTCCTGTAACGACAACAGTGAGCTCGATTCTGAATCCTGAATCGCCGACTGGTCAGGCTGCTAAGAAAAAGAGTGCAAAAGAATGGAACTTGCCGTCTAGATATCCTTCAGACACCAAGAGTGAGAAGAGGAAAGTAAAGAGAAGATCCTGGGCTCAATTTGTGTGCTGCGCATTTGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.42

Weight (kDa)

10.05

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 409
AciI CCGC 1 cut(s) 49
AclWI GGATC 1 cut(s) 378
AcuI CTGAAG 1 cut(s) 330
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 1 cut(s) 130
AjnI CCWGG 1 cut(s) 386
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 3 cut(s) 29, 224, 261
AluI AGCT 3 cut(s) 29, 224, 261
Alw21I GWGCWC 2 cut(s) 107, 263
Alw26I GTCTC 2 cut(s) 8, 71
AlwI GGATC 1 cut(s) 378
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 3 cut(s) 224, 296, 405
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 239
BanII GRGCYC 2 cut(s) 263, 394
Bbv12I GWGCWC 2 cut(s) 107, 263
BbvI GCAGC 3 cut(s) 211, 283, 392
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 8, 71
BfaI CTAG 2 cut(s) 11, 336
BfmI CTRYAG 1 cut(s) 32
BisI GCNGC 3 cut(s) 225, 297, 406
BlsI GCNGC 3 cut(s) 226, 298, 407
Bme1390I CCNGG 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 388
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 13
BmuI ACTGGG 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 112, 292
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 1 cut(s) 387
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 2 cut(s) 112, 292
BseXI GCAGC 3 cut(s) 211, 283, 392
BshFI GGCC 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 107, 263
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 2 cut(s) 8, 71
BsnI GGCC 1 cut(s) 149
Bso31I GGTCTC 1 cut(s) 71
Bsp1286I GDGCHC 3 cut(s) 107, 263, 394
Bsp143I GATC 1 cut(s) 383
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 1 cut(s) 199
BspPI GGATC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 81
BsrI ACTGG 2 cut(s) 112, 292
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 1 cut(s) 383
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 2 cut(s) 69, 359
BstDEI CTNAG 3 cut(s) 87, 207, 300
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 1 cut(s) 386
BstMAI GTCTC 2 cut(s) 8, 71
BstMBI GATC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 388
BstSCI CCNGG 1 cut(s) 386
BstSFI CTRYAG 1 cut(s) 32
BstV1I GCAGC 3 cut(s) 211, 283, 392
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 1 cut(s) 149
BtsIMutI CAGTG 4 cut(s) 105, 173, 241, 261
CfoI GCGC 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 239
CviJI RGCY 8 cut(s) 29, 135, 149, 172, 224, 261, 296, 392
CviKI_1 RGCY 8 cut(s) 29, 135, 149, 172, 224, 261, 296, 392
DdeI CTNAG 3 cut(s) 87, 207, 300
DpnI GATC 1 cut(s) 385
DpnII GATC 1 cut(s) 383
Eam1104I CTCTTC 2 cut(s) 69, 359
EarI CTCTTC 2 cut(s) 69, 359
Ecl136II GAGCTC 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 2 cut(s) 263, 394
Eco31I GGTCTC 1 cut(s) 71
Eco32I GATATC 1 cut(s) 341
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 386
EcoRV GATATC 1 cut(s) 341
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 263, 394
FalI AAGNNNNNCTT 2 cut(s) 177, 209
Fnu4HI GCNGC 3 cut(s) 225, 297, 406
FriOI GRGCYC 2 cut(s) 263, 394
Fsp4HI GCNGC 3 cut(s) 225, 297, 406
FspBI CTAG 2 cut(s) 11, 336
FspI TGCGCA 1 cut(s) 409
GlaI GCGC 1 cut(s) 409
GluI GCNGC 3 cut(s) 225, 297, 406
HaeIII GGCC 1 cut(s) 149
HhaI GCGC 1 cut(s) 410
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HinfI GANTC 4 cut(s) 55, 265, 271, 278
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 270, 349
Hpy188III TCNNGA 4 cut(s) 11, 206, 275, 336
HpyAV CCTTC 1 cut(s) 354
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4V TGCA 3 cut(s) 4, 227, 314
HpyF3I CTNAG 3 cut(s) 87, 207, 300
HspAI GCGC 1 cut(s) 408
Kzo9I GATC 1 cut(s) 383
LmnI GCTCC 3 cut(s) 102, 202, 208
LpnPI CCDG 9 cut(s) 93, 213, 219, 255, 273, 278, 288, 373, 400
Lsp1109I GCAGC 3 cut(s) 211, 283, 392
LweI GCATC 1 cut(s) 13
MaeI CTAG 2 cut(s) 11, 336
MaeIII GTNAC 2 cut(s) 91, 244
MalI GATC 1 cut(s) 385
MboI GATC 1 cut(s) 383
MboII GAAGA 4 cut(s) 79, 86, 376, 393
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 3 cut(s) 107, 263, 394
MluCI AATT 2 cut(s) 395, 418
MlyI GAGTC 1 cut(s) 49
MnlI CCTC 4 cut(s) 45, 139, 160, 360
Mph1103I ATGCAT 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 388
MvaI CCWGG 1 cut(s) 388
NdeII GATC 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 91
NsbI TGCGCA 1 cut(s) 409
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 149
PfeI GAWTC 3 cut(s) 265, 271, 278
PkrI GCNGC 3 cut(s) 226, 298, 407
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 386
PspGI CCWGG 1 cut(s) 386
PspPI GGNCC 1 cut(s) 239
PsuI RGATCY 1 cut(s) 383
SacI GAGCTC 1 cut(s) 263
SatI GCNGC 3 cut(s) 225, 297, 406
Sau3AI GATC 1 cut(s) 383
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 1 cut(s) 388
SduI GDGCHC 3 cut(s) 107, 263, 394
SetI ASST 6 cut(s) 31, 46, 93, 226, 234, 263
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 32
SinI GGWCC 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 2 cut(s) 395, 418
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 1 cut(s) 49
SspMI CTAG 2 cut(s) 11, 336
SstI GAGCTC 1 cut(s) 263
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 256
TaqI TCGA 1 cut(s) 263
TasI AATT 2 cut(s) 395, 418
TfiI GAWTC 3 cut(s) 265, 271, 278
TscAI CASTG 4 cut(s) 112, 180, 241, 261
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 3 cut(s) 224, 296, 405
Tsp45I GTSAC 1 cut(s) 91
TspRI CASTG 4 cut(s) 112, 180, 241, 261
VpaK11BI GGWCC 1 cut(s) 239
XbaI TCTAGA 2 cut(s) 10, 335
XspI CTAG 2 cut(s) 11, 336
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.