Rh4DG169300

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001352
Physical Location & Seq
Reverse (-)
33686065 .. 33691731
5667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG169300.1

Sequence Viewer

Length: 1992 bp
ATGGATCACCAAGATCTCAGGCGAGCCCACAGTTCAGGGCACGAGAGTCACCATGTGTGTCACAAATGCGGGTGGCCTTTTCCGAATCCGCACCCGAGTGCTAGACACAGACGCGCGCATAAGAAGATTTGCGGAACCGTTGAGGGTTACAAGGTGGCTGGCGCTGAGGGGAGTGCACAGTTGAGTGTCTCTGATGACGAGCAGCACTCTGATGAAGATCCCAAAACCCCCGGTCCGAAAGTCTTGGAAAGGAGCGTTTATGAGAGGGGTAGTGGCGGAATTGGGCAAGGATCTATTAAATCAGATGATGGGGTGTTCTCAGATGCTGTTCAGGAGTTTTCAGATAGCGGTTCTGCTGCATGGACCGGAGAAGGGTTGGAGGATGCCCCGGGATCAGCACCTAATGTAGAAAGGGGTGCCAAGAGTGATTTGAATGCCATTCCGTTCCACAATGATGGGGGAACGACAGGTAAACCTTCCAATGAAATAAGAACTGATATTAGATATCTTCATGTGTCTTTGAGAAAAGAGAAAAATGATATTAGATTTGATAAAACCGAGAAGTGTGATATAACTGGGTATGATGGTGATCATGAGAAAGCTGAAAACTATGCATCATCTGGTGAAGAAATAAGTAAAAGATCTACAGAAAAAACCTTTGCGGAGGACTCTACCACTAATTTGGAATCCATCATTACTCTCTCCGACTCTCAAGTTCATCCATCGGGAAAGTTGCCTGATGTTGATGGCTCTGCTGATCGTGAAGAGGGTGAAATTGAGAAATTTGTTATATCTGGAAATGAAAGCATGGAAGGATCAGTTGAAGAAAATATTCTGGTGAAGTCAGAATTGACCCCCGAGTCTTCTGCCAATCTAACTGGTTCAAAAGCTATTGTTGAAGATGCAGCCAATGGTTCTAGGATGGAGCTGGTTGAAACCAGTGGCAGAGATTTAGATAGCGGTTCGAATTCTAGCAAAGTTGCAGGAAAAATTCTATCAGATGCTCAGCAAGATGTGTTAGAAAATGAGATTGCTTGCAATGACAAAATCCTGAAAGAAGTTGTTGCCGGTGTTGCTGCTGGGTCAGATCATGGTCAGAATGTTGAATCGTTCCAGAAGAGTTCGGAGGACCATGGGAAGAAAGAACCAGGGTTTTCTGCTTTAGATACCGAATCTTCTGTTCAAAGTTTCATTGCCTCTGAAGATAGCGGCTCCCAAGAGGTCCATGCGGTTGCTTCTGGGATTACTTCTCAATCTTTGTTAGAGGAAGGCGATAATAACCTTGTCAGACATCAGCCAAGCTCAACTGCAATTGATGTGGACTCAAATAGCCAAACTGACAGTCTGGAAGCCAACTGGGGATCTTTCTCAGTTCTTTCCATGCAATCTGATGCTGAAACTGTGCCACATACGCATTCCCAATCTTCATTAGGTGAAAACTCGGGCAAATCTGATATGTTTGAGGCACCATCTTTCATGACATTGGTTGAACCCCGAGGTGGGGATGATCAAAAAGCTACTGGTTCTGAAATCCTGACTGCAGTACAGAACCCAGAACAGCCAAAACCTGCATCTTTGCAAACTGGGTGGTTTCCTTCTATGACTCATGTTGTGAATGAGTCACCGGGGAGAAAGAAGAACGAAGAGATCATTGCCAAGGTGACAAACAGGAGCACTGGGAAGCAACACACTCCTTTGAAAAACCTTTTGGGGGAGGCCTCAAGTGAAAGCAACGGAAAGCCACTGAACCAAAAAGAAAGTCTTGCTCTCGCTCCTCAGAAAGATGATAAAGCTGCAATGGTCAGTGGTCCTGTAACAACAACAGTGAGCTCGATTCTGAATCCTGAATCGCCGACTGGTCAGGCTGCTAAGAAAGAGACTGCAAAAGAATGGAACTCGCCGGCCAGATATCCTTCAGACACTAAGAGTGAGAAGAGGAAGGTAAAGGGGAGATCCTGGGCACAATTTGTGTGCTGCGCTTCTGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

663

Amino Acids

70.93

Weight (kDa)

5.0

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 859
Acc36I ACCTGC 1 cut(s) 1576
AccB1I GGYRCC 2 cut(s) 416, 1465
AccII CGCG 2 cut(s) 114, 116
AciI CCGC 9 cut(s) 69, 89, 132, 276, 348, 662, 960, 1209, 1229
AclWI GGATC 7 cut(s) 12, 212, 298, 400, 823, 1369, 1947
AcoI YGGCCR 1 cut(s) 1902
AcsI RAATTY 3 cut(s) 782, 967, 990
AcuI CTGAAG 2 cut(s) 1221, 1899
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 1 cut(s) 1545
AfiI CCNNNNNNNGG 5 cut(s) 372, 1499, 1500, 1501, 1711
AgsI TTSAA 9 cut(s) 433, 824, 885, 899, 935, 1106, 1184, 1490, 1699
AjnI CCWGG 2 cut(s) 1147, 1955
AjuI GAANNNNNNNTTGG 2 cut(s) 1691, 1723
AleI CACNNNNGTG 1 cut(s) 96
AluBI AGCT 7 cut(s) 602, 890, 928, 1302, 1517, 1793, 1830
AluI AGCT 7 cut(s) 602, 890, 928, 1302, 1517, 1793, 1830
Alw21I GWGCWC 3 cut(s) 178, 1676, 1832
Alw26I GTCTC 2 cut(s) 193, 1871
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 7 cut(s) 12, 212, 298, 400, 823, 1369, 1947
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 5 cut(s) 94, 388, 857, 1441, 1494
AoxI GGCC 3 cut(s) 74, 1716, 1902
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 7 cut(s) 202, 356, 905, 1076, 1793, 1865, 1974
ApoI RAATTY 3 cut(s) 782, 967, 990
ArsI GACNNNNNNTTYG 4 cut(s) 1327, 1359, 1744, 1776
Asp700I GAANNNNTTC 1 cut(s) 831
AspLEI GCGC 4 cut(s) 116, 118, 164, 1979
AspS9I GGNCC 5 cut(s) 233, 363, 1129, 1222, 1808
AsuC2I CCSGG 4 cut(s) 231, 389, 390, 1626
AsuHPI GGTGA 8 cut(s) 41, 599, 635, 782, 850, 1445, 1614, 1672
AsuII TTCGAA 1 cut(s) 965
AvaI CYCGRG 5 cut(s) 94, 388, 857, 1441, 1494
AvaII GGWCC 5 cut(s) 233, 363, 1129, 1222, 1808
BaeGI GKGCMC 3 cut(s) 42, 178, 1963
BanI GGYRCC 2 cut(s) 416, 1465
BanII GRGCYC 2 cut(s) 28, 1832
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 3 cut(s) 178, 1676, 1832
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 7 cut(s) 214, 343, 917, 1063, 1780, 1852, 1961
BccI CCATC 8 cut(s) 302, 449, 578, 698, 730, 740, 916, 1477
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 2 cut(s) 1149, 1957
BclI TGATCA 2 cut(s) 589, 1507
BcnI CCSGG 4 cut(s) 231, 389, 390, 1626
BcoDI GTCTC 2 cut(s) 193, 1871
BfaI CTAG 3 cut(s) 102, 918, 972
BfmI CTRYAG 2 cut(s) 645, 1539
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 1576
BglII AGATCT 2 cut(s) 13, 641
BisI GCNGC 8 cut(s) 203, 357, 906, 1077, 1210, 1794, 1866, 1975
BlpI GCTNAGC 1 cut(s) 1005
BlsI GCNGC 8 cut(s) 204, 358, 907, 1078, 1211, 1795, 1867, 1976
Bme1390I CCNGG 6 cut(s) 231, 389, 390, 1149, 1626, 1957
Bme18I GGWCC 5 cut(s) 233, 363, 1129, 1222, 1808
BmeT110I CYCGRG 5 cut(s) 94, 388, 857, 1441, 1494
BmgT120I GGNCC 5 cut(s) 233, 363, 1129, 1222, 1808
BmiI GGNNCC 4 cut(s) 136, 418, 1213, 1467
BmrFI CCNGG 6 cut(s) 231, 389, 390, 1149, 1626, 1957
BmrI ACTGGG 4 cut(s) 585, 1366, 1593, 1686
BmsI GCATC 7 cut(s) 313, 373, 623, 892, 991, 1381, 1580
BmuI ACTGGG 4 cut(s) 585, 1366, 1593, 1686
BpiI GAAGAC 1 cut(s) 855
BplI GAGNNNNNCTC 2 cut(s) 191, 223
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 1005
Bpu14I TTCGAA 1 cut(s) 965
BpuEI CTTGAG 2 cut(s) 696, 1705
BpuMI CCSGG 4 cut(s) 231, 389, 390, 1626
BsaBI GATNNNNATC 2 cut(s) 216, 588
BsaJI CCNNGG 9 cut(s) 229, 387, 388, 1132, 1148, 1495, 1625, 1656, 1956
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 1499, 1500, 1501, 1711
Bse118I RCCGGY 2 cut(s) 1067, 1900
Bse1I ACTGG 8 cut(s) 580, 883, 939, 1361, 1525, 1588, 1681, 1861
Bse3DI GCAATG 4 cut(s) 1045, 1191, 1650, 1803
Bse8I GATNNNNATC 2 cut(s) 216, 588
BseBI CCWGG 2 cut(s) 1149, 1957
BseDI CCNNGG 9 cut(s) 229, 387, 388, 1132, 1148, 1495, 1625, 1656, 1956
BseGI GGATG 4 cut(s) 388, 718, 927, 1510
BseJI GATNNNNATC 2 cut(s) 216, 588
BseLI CCNNNNNNNGG 5 cut(s) 372, 1499, 1500, 1501, 1711
BseMI GCAATG 4 cut(s) 1045, 1191, 1650, 1803
BseMII CTCAG 6 cut(s) 31, 156, 333, 1019, 1383, 1790
BseNI ACTGG 8 cut(s) 580, 883, 939, 1361, 1525, 1588, 1681, 1861
BsePI GCGCGC 1 cut(s) 114
BseRI GAGGAG 1 cut(s) 1764
BseSI GKGCMC 3 cut(s) 42, 178, 1963
BseXI GCAGC 7 cut(s) 214, 343, 917, 1063, 1780, 1852, 1961
BseYI CCCAGC 1 cut(s) 1079
Bsh1236I CGCG 2 cut(s) 114, 116
BshFI GGCC 3 cut(s) 76, 1718, 1904
BshNI GGYRCC 2 cut(s) 416, 1465
BsiHKAI GWGCWC 3 cut(s) 178, 1676, 1832
BsiHKCI CYCGRG 5 cut(s) 94, 388, 857, 1441, 1494
BsiSI CCGG 6 cut(s) 231, 366, 389, 1068, 1625, 1901
BslI CCNNNNNNNGG 5 cut(s) 372, 1499, 1500, 1501, 1711
BsmAI GTCTC 2 cut(s) 193, 1871
BsmI GAATGC 2 cut(s) 439, 1414
BsnI GGCC 3 cut(s) 76, 1718, 1904
BsoBI CYCGRG 5 cut(s) 94, 388, 857, 1441, 1494
Bsp119I TTCGAA 1 cut(s) 965
Bsp1286I GDGCHC 6 cut(s) 28, 42, 178, 1676, 1832, 1963
Bsp1720I GCTNAGC 1 cut(s) 1005
Bsp19I CCATGG 1 cut(s) 1132
BspACI CCGC 9 cut(s) 69, 89, 132, 276, 348, 662, 960, 1209, 1229
BspANI GGCC 3 cut(s) 76, 1718, 1904
BspCNI CTCAG 6 cut(s) 30, 157, 332, 1018, 1382, 1789
BspFNI CGCG 2 cut(s) 114, 116
BspHI TCATGA 2 cut(s) 592, 1476
BspLI GGNNCC 4 cut(s) 136, 418, 1213, 1467
BspMAI CTGCAG 1 cut(s) 1543
BspMI ACCTGC 1 cut(s) 1576
BspPI GGATC 7 cut(s) 12, 212, 298, 400, 823, 1369, 1947
BspT104I TTCGAA 1 cut(s) 965
BspT107I GGYRCC 2 cut(s) 416, 1465
BsrDI GCAATG 4 cut(s) 1045, 1191, 1650, 1803
BsrFI RCCGGY 2 cut(s) 1067, 1900
BsrI ACTGG 8 cut(s) 580, 883, 939, 1361, 1525, 1588, 1681, 1861
BssAI RCCGGY 2 cut(s) 1067, 1900
BssECI CCNNGG 9 cut(s) 229, 387, 388, 1132, 1148, 1495, 1625, 1656, 1956
BssHII GCGCGC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 2 cut(s) 1132, 1656
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 2 cut(s) 1149, 1957
Bst4CI ACNGT 6 cut(s) 32, 139, 180, 1343, 1402, 1825
Bst6I CTCTTC 4 cut(s) 759, 1112, 1638, 1928
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 5 cut(s) 24, 116, 160, 1036, 1902
BstDEI CTNAG 8 cut(s) 17, 165, 319, 1005, 1369, 1776, 1869, 1923
BstDSI CCRYGG 1 cut(s) 1132
BstF5I GGATG 4 cut(s) 388, 718, 927, 1510
BstFNI CGCG 2 cut(s) 114, 116
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 116, 118, 164, 1979
BstMAI GTCTC 2 cut(s) 193, 1871
BstMWI GCNNNNNNNGC 2 cut(s) 1073, 1411
BstNI CCWGG 2 cut(s) 1149, 1957
BstSCI CCNGG 6 cut(s) 229, 387, 388, 1147, 1624, 1955
BstSFI CTRYAG 2 cut(s) 645, 1539
BstSLI GKGCMC 3 cut(s) 42, 178, 1963
BstUI CGCG 2 cut(s) 114, 116
BstV1I GCAGC 7 cut(s) 214, 343, 917, 1063, 1780, 1852, 1961
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 6 cut(s) 13, 217, 290, 641, 1361, 1952
BstXI CCANNNNNNTGG 2 cut(s) 455, 682
BstYI RGATCY 6 cut(s) 13, 217, 290, 641, 1361, 1952
BsuRI GGCC 3 cut(s) 76, 1718, 1904
BtgI CCRYGG 1 cut(s) 1132
BtsCI GGATG 4 cut(s) 388, 718, 927, 1510
BtsIMutI CAGTG 5 cut(s) 946, 1674, 1742, 1810, 1830
BveI ACCTGC 1 cut(s) 1576
Cac8I GCNNGC 5 cut(s) 24, 116, 160, 1036, 1902
CaiI CAGNNNCTG 1 cut(s) 326
CciI TCATGA 2 cut(s) 592, 1476
CfoI GCGC 4 cut(s) 116, 118, 164, 1979
Cfr10I RCCGGY 2 cut(s) 1067, 1900
Cfr13I GGNCC 5 cut(s) 233, 363, 1129, 1222, 1808
Cfr9I CCCGGG 1 cut(s) 388
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 1544
CspCI CAANNNNNGTGG 4 cut(s) 1299, 1334, 1568, 1603
CspI CGGWCCG 1 cut(s) 233
CviQI GTAC 1 cut(s) 1544
DdeI CTNAG 8 cut(s) 17, 165, 319, 1005, 1369, 1776, 1869, 1923
DraIII CACNNNGTG 1 cut(s) 55
DrdI GACNNNNNNGTC 1 cut(s) 859
DseDI GACNNNNNNGTC 1 cut(s) 859
EaeI YGGCCR 1 cut(s) 1902
Eam1104I CTCTTC 4 cut(s) 759, 1112, 1638, 1928
EarI CTCTTC 4 cut(s) 759, 1112, 1638, 1928
EciI GGCGGA 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 1830
Eco130I CCWWGG 2 cut(s) 1132, 1656
Eco147I AGGCCT 1 cut(s) 1718
Eco24I GRGCYC 2 cut(s) 28, 1832
Eco32I GATATC 2 cut(s) 506, 1910
Eco47I GGWCC 5 cut(s) 233, 363, 1129, 1222, 1808
Eco53kI GAGCTC 1 cut(s) 1830
Eco57I CTGAAG 2 cut(s) 1221, 1899
Eco88I CYCGRG 5 cut(s) 94, 388, 857, 1441, 1494
EcoICRI GAGCTC 1 cut(s) 1830
EcoRI GAATTC 1 cut(s) 967
EcoRII CCWGG 2 cut(s) 1147, 1955
EcoRV GATATC 2 cut(s) 506, 1910
EcoT14I CCWWGG 2 cut(s) 1132, 1656
EcoT22I ATGCAT 1 cut(s) 616
EcoT38I GRGCYC 2 cut(s) 28, 1832
ErhI CCWWGG 2 cut(s) 1132, 1656
FalI AAGNNNNNCTT 2 cut(s) 1746, 1778
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 2 cut(s) 589, 1507
Fnu4HI GCNGC 8 cut(s) 203, 357, 906, 1077, 1210, 1794, 1866, 1975
FokI GGATG 4 cut(s) 395, 705, 934, 1517
FriOI GRGCYC 2 cut(s) 28, 1832
Fsp4HI GCNGC 8 cut(s) 203, 357, 906, 1077, 1210, 1794, 1866, 1975
FspBI CTAG 3 cut(s) 102, 918, 972
GlaI GCGC 4 cut(s) 115, 117, 163, 1978
GluI GCNGC 8 cut(s) 203, 357, 906, 1077, 1210, 1794, 1866, 1975
GsaI CCCAGC 1 cut(s) 1083
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 76, 1718, 1904
HapII CCGG 6 cut(s) 231, 366, 389, 1068, 1625, 1901
HgaI GACGC 1 cut(s) 120
HhaI GCGC 4 cut(s) 116, 118, 164, 1979
Hin6I GCGC 4 cut(s) 114, 116, 162, 1977
HinP1I GCGC 4 cut(s) 114, 116, 162, 1977
HpaII CCGG 6 cut(s) 231, 366, 389, 1068, 1625, 1901
HphI GGTGA 8 cut(s) 41, 599, 635, 782, 850, 1445, 1614, 1672
Hpy166II GTNNAC 3 cut(s) 176, 473, 1321
Hpy8I GTNNAC 3 cut(s) 176, 473, 1321
HpyAV CCTTC 7 cut(s) 365, 486, 806, 1262, 1605, 1923, 1933
HpyCH4III ACNGT 6 cut(s) 32, 139, 180, 1343, 1402, 1825
HpyF10VI GCNNNNNNNGC 2 cut(s) 1073, 1411
HpyF3I CTNAG 8 cut(s) 17, 165, 319, 1005, 1369, 1776, 1869, 1923
HspAI GCGC 4 cut(s) 114, 116, 162, 1977
KroI GCCGGC 1 cut(s) 1900
KroNI GCCGGC 1 cut(s) 1902
Ksp22I TGATCA 2 cut(s) 589, 1507
LmnI GCTCC 5 cut(s) 252, 925, 1217, 1671, 1777
Lsp1109I GCAGC 7 cut(s) 214, 343, 917, 1063, 1780, 1852, 1961
LweI GCATC 7 cut(s) 313, 373, 623, 892, 991, 1381, 1580
MaeI CTAG 3 cut(s) 102, 918, 972
MaeIII GTNAC 6 cut(s) 47, 59, 146, 1620, 1660, 1813
MfeI CAATTG 1 cut(s) 1311
MflI RGATCY 6 cut(s) 13, 217, 290, 641, 1361, 1952
MhlI GDGCHC 6 cut(s) 28, 42, 178, 1676, 1832, 1963
MlyI GAGTC 7 cut(s) 55, 662, 701, 869, 1316, 1597, 1628
MmeI TCCRAC 2 cut(s) 357, 729
Mph1103I ATGCAT 1 cut(s) 616
MroNI GCCGGC 1 cut(s) 1900
MroXI GAANNNNTTC 1 cut(s) 831
MseI TTAA 1 cut(s) 297
MslI CAYNNNNRTG 3 cut(s) 96, 210, 453
MspI CCGG 6 cut(s) 231, 366, 389, 1068, 1625, 1901
MspR9I CCNGG 6 cut(s) 231, 389, 390, 1149, 1626, 1957
MunI CAATTG 1 cut(s) 1311
Mva1269I GAATGC 2 cut(s) 439, 1414
MvaI CCWGG 2 cut(s) 1149, 1957
MvnI CGCG 2 cut(s) 114, 116
MwoI GCNNNNNNNGC 2 cut(s) 1073, 1411
NaeI GCCGGC 1 cut(s) 1902
NciI CCSGG 4 cut(s) 231, 389, 390, 1626
NcoI CCATGG 1 cut(s) 1132
NgoMIV GCCGGC 1 cut(s) 1900
NlaIV GGNNCC 4 cut(s) 136, 418, 1213, 1467
NmuCI GTSAC 4 cut(s) 47, 59, 1620, 1660
NsiI ATGCAT 1 cut(s) 616
NspV TTCGAA 1 cut(s) 965
OliI CACNNNNGTG 1 cut(s) 96
PagI TCATGA 2 cut(s) 592, 1476
PauI GCGCGC 1 cut(s) 114
PceI AGGCCT 1 cut(s) 1718
PctI GAATGC 2 cut(s) 439, 1414
PdiI GCCGGC 1 cut(s) 1902
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 7 cut(s) 85, 686, 1106, 1172, 1834, 1840, 1847
PkrI GCNGC 8 cut(s) 204, 358, 907, 1078, 1211, 1795, 1867, 1976
PleI GAGTC 7 cut(s) 54, 662, 701, 868, 1316, 1597, 1627
PpsI GAGTC 7 cut(s) 54, 662, 701, 868, 1316, 1597, 1627
Psp124BI GAGCTC 1 cut(s) 1832
Psp6I CCWGG 2 cut(s) 1147, 1955
PspFI CCCAGC 1 cut(s) 1079
PspGI CCWGG 2 cut(s) 1147, 1955
PspN4I GGNNCC 4 cut(s) 136, 418, 1213, 1467
PspPI GGNCC 5 cut(s) 233, 363, 1129, 1222, 1808
PstI CTGCAG 1 cut(s) 1543
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 6 cut(s) 13, 217, 290, 641, 1361, 1952
PteI GCGCGC 1 cut(s) 114
RsaI GTAC 1 cut(s) 1545
RsaNI GTAC 1 cut(s) 1544
RseI CAYNNNNRTG 3 cut(s) 96, 210, 453
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 1832
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 8 cut(s) 203, 357, 906, 1077, 1210, 1794, 1866, 1975
Sau96I GGNCC 5 cut(s) 233, 363, 1129, 1222, 1808
SchI GAGTC 7 cut(s) 55, 662, 701, 869, 1316, 1597, 1628
ScrFI CCNGG 6 cut(s) 231, 389, 390, 1149, 1626, 1957
SduI GDGCHC 6 cut(s) 28, 42, 178, 1676, 1832, 1963
SfaNI GCATC 7 cut(s) 313, 373, 623, 892, 991, 1381, 1580
SfcI CTRYAG 2 cut(s) 645, 1539
SfuI TTCGAA 1 cut(s) 965
SinI GGWCC 5 cut(s) 233, 363, 1129, 1222, 1808
SmaI CCCGGG 1 cut(s) 390
SmiMI CAYNNNNRTG 3 cut(s) 96, 210, 453
SmlI CTYRAG 2 cut(s) 711, 1720
SmoI CTYRAG 2 cut(s) 711, 1720
SseBI AGGCCT 1 cut(s) 1718
SsiI CCGC 9 cut(s) 69, 89, 132, 276, 348, 662, 960, 1209, 1229
SspI AATATT 1 cut(s) 832
SspMI CTAG 3 cut(s) 102, 918, 972
SstI GAGCTC 1 cut(s) 1832
StuI AGGCCT 1 cut(s) 1718
StyD4I CCNGG 6 cut(s) 229, 387, 388, 1147, 1624, 1955
StyI CCWWGG 2 cut(s) 1132, 1656
TaaI ACNGT 6 cut(s) 32, 139, 180, 1343, 1402, 1825
TaqI TCGA 2 cut(s) 965, 1832
TatI WGTACW 1 cut(s) 1543
TauI GCSGC 1 cut(s) 1212
TfiI GAWTC 7 cut(s) 85, 686, 1106, 1172, 1834, 1840, 1847
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 5 cut(s) 946, 1681, 1749, 1810, 1830
TseFI GTSAC 4 cut(s) 47, 59, 1620, 1660
TseI GCWGC 7 cut(s) 202, 356, 905, 1076, 1793, 1865, 1974
Tsp45I GTSAC 4 cut(s) 47, 59, 1620, 1660
TspDTI ATGAA 8 cut(s) 228, 498, 500, 707, 816, 1180, 1416, 1465
TspGWI ACGGA 2 cut(s) 432, 1749
TspMI CCCGGG 1 cut(s) 388
TspRI CASTG 5 cut(s) 946, 1681, 1749, 1810, 1830
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 5 cut(s) 233, 363, 1129, 1222, 1808
XapI RAATTY 3 cut(s) 782, 967, 990
XmaI CCCGGG 1 cut(s) 388
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 3 cut(s) 102, 918, 972
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.