endonuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2779 |
| Acc16I | TGCGCA | 1 cut(s) | 3952 |
| Acc36I | ACCTGC | 1 cut(s) | 3538 |
| AccB1I | GGYRCC | 4 cut(s) | 161, 416, 761, 3427 |
| AccII | CGCG | 2 cut(s) | 114, 116 |
| AcoI | YGGCCR | 2 cut(s) | 3013, 3876 |
| AcsI | RAATTY | 5 cut(s) | 2199, 2353, 2702, 2887, 2910 |
| AcyI | GRCGYC | 1 cut(s) | 162 |
| AdeI | CACNNNGTG | 1 cut(s) | 55 |
| AfaI | GTAC | 3 cut(s) | 1167, 2167, 3507 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 1420, 1427, 3461, 3462, 3463, 3673 |
| AflIII | ACRYGT | 3 cut(s) | 1039, 1168, 1195 |
| AjnI | CCWGG | 3 cut(s) | 579, 3067, 3929 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3653, 3685 |
| AleI | CACNNNNGTG | 1 cut(s) | 96 |
| Alw21I | GWGCWC | 3 cut(s) | 178, 3638, 3806 |
| Alw26I | GTCTC | 7 cut(s) | 193, 1387, 1481, 1731, 1890, 2106, 3845 |
| Alw44I | GTGCAC | 1 cut(s) | 174 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 326, 1547, 1952, 2369 |
| Ama87I | CYCGRG | 6 cut(s) | 94, 388, 2777, 3403, 3456, 3682 |
| AoxI | GGCC | 4 cut(s) | 74, 3013, 3678, 3876 |
| ApaLI | GTGCAC | 1 cut(s) | 174 |
| ApoI | RAATTY | 5 cut(s) | 2199, 2353, 2702, 2887, 2910 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 3348, 3380, 3706, 3738 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 954, 1346, 2751 |
| AspLEI | GCGC | 4 cut(s) | 116, 118, 164, 3953 |
| AspS9I | GGNCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| AsuC2I | CCSGG | 4 cut(s) | 231, 389, 390, 3588 |
| AsuII | TTCGAA | 2 cut(s) | 236, 2885 |
| AsuNHI | GCTAGC | 1 cut(s) | 1409 |
| AvaI | CYCGRG | 6 cut(s) | 94, 388, 2777, 3403, 3456, 3682 |
| AvaII | GGWCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| BaeGI | GKGCMC | 3 cut(s) | 42, 178, 3937 |
| BalI | TGGCCA | 1 cut(s) | 3015 |
| BanI | GGYRCC | 4 cut(s) | 161, 416, 761, 3427 |
| BanII | GRGCYC | 3 cut(s) | 28, 1537, 3806 |
| BauI | CACGAG | 1 cut(s) | 41 |
| BbsI | GAAGAC | 3 cut(s) | 477, 536, 2775 |
| Bbv12I | GWGCWC | 3 cut(s) | 178, 3638, 3806 |
| BceAI | ACGGC | 1 cut(s) | 3145 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 155, 189, 2768, 2802 |
| BciT130I | CCWGG | 3 cut(s) | 581, 3069, 3931 |
| BclI | TGATCA | 4 cut(s) | 802, 2182, 2509, 3469 |
| BcnI | CCSGG | 4 cut(s) | 231, 389, 390, 3588 |
| BcoDI | GTCTC | 7 cut(s) | 193, 1387, 1481, 1731, 1890, 2106, 3845 |
| BfaI | CTAG | 5 cut(s) | 102, 842, 1410, 2838, 2892 |
| BfmI | CTRYAG | 8 cut(s) | 1758, 1785, 1917, 1944, 2116, 2160, 2565, 3501 |
| BfoI | RGCGCY | 1 cut(s) | 165 |
| BfuAI | ACCTGC | 1 cut(s) | 3538 |
| BglII | AGATCT | 2 cut(s) | 13, 2561 |
| BlpI | GCTNAGC | 1 cut(s) | 2925 |
| Bme1390I | CCNGG | 7 cut(s) | 231, 389, 390, 581, 3069, 3588, 3931 |
| Bme18I | GGWCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| BmeT110I | CYCGRG | 6 cut(s) | 94, 388, 2777, 3403, 3456, 3682 |
| BmgT120I | GGNCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| BmiI | GGNNCC | 8 cut(s) | 136, 163, 418, 763, 1534, 2131, 3133, 3429 |
| BmrFI | CCNGG | 7 cut(s) | 231, 389, 390, 581, 3069, 3588, 3931 |
| BmrI | ACTGGG | 4 cut(s) | 2505, 3286, 3555, 3648 |
| BmtI | GCTAGC | 1 cut(s) | 1413 |
| BmuI | ACTGGG | 4 cut(s) | 2505, 3286, 3555, 3648 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1975 |
| BpiI | GAAGAC | 3 cut(s) | 477, 536, 2775 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 191, 223, 881, 913 |
| BpmI | CTGGAG | 4 cut(s) | 387, 1755, 1914, 2130 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2925 |
| Bpu14I | TTCGAA | 2 cut(s) | 236, 2885 |
| BpuEI | CTTGAG | 1 cut(s) | 2616 |
| BpuMI | CCSGG | 4 cut(s) | 231, 389, 390, 3588 |
| BsaAI | YACGTR | 1 cut(s) | 2435 |
| BsaBI | GATNNNNATC | 2 cut(s) | 216, 2508 |
| BsaHI | GRCGYC | 1 cut(s) | 162 |
| BsaI | GGTCTC | 3 cut(s) | 1481, 1731, 1890 |
| BsaWI | WCCGGW | 2 cut(s) | 1420, 1841 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 1420, 1427, 3461, 3462, 3463, 3673 |
| Bse118I | RCCGGY | 2 cut(s) | 2987, 3874 |
| Bse3DI | GCAATG | 5 cut(s) | 703, 2965, 3111, 3612, 3777 |
| Bse8I | GATNNNNATC | 2 cut(s) | 216, 2508 |
| BseBI | CCWGG | 3 cut(s) | 581, 3069, 3931 |
| BseGI | GGATG | 6 cut(s) | 388, 814, 2332, 2638, 2847, 3472 |
| BseJI | GATNNNNATC | 2 cut(s) | 216, 2508 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 1420, 1427, 3461, 3462, 3463, 3673 |
| BseMI | GCAATG | 5 cut(s) | 703, 2965, 3111, 3612, 3777 |
| BsePI | GCGCGC | 1 cut(s) | 114 |
| BseRI | GAGGAG | 1 cut(s) | 3738 |
| BseSI | GKGCMC | 3 cut(s) | 42, 178, 3937 |
| BseYI | CCCAGC | 1 cut(s) | 2999 |
| Bsh1236I | CGCG | 2 cut(s) | 114, 116 |
| BshFI | GGCC | 4 cut(s) | 76, 3015, 3680, 3878 |
| BshNI | GGYRCC | 4 cut(s) | 161, 416, 761, 3427 |
| BsiHKAI | GWGCWC | 3 cut(s) | 178, 3638, 3806 |
| BsiHKCI | CYCGRG | 6 cut(s) | 94, 388, 2777, 3403, 3456, 3682 |
| BsiSI | CCGG | 7 cut(s) | 231, 389, 1421, 1842, 2988, 3587, 3875 |
| BslFI | GGGAC | 1 cut(s) | 652 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 1420, 1427, 3461, 3462, 3463, 3673 |
| BsmAI | GTCTC | 7 cut(s) | 193, 1387, 1481, 1731, 1890, 2106, 3845 |
| BsmFI | GGGAC | 1 cut(s) | 652 |
| BsmI | GAATGC | 3 cut(s) | 439, 1156, 3334 |
| BsnI | GGCC | 4 cut(s) | 76, 3015, 3680, 3878 |
| Bso31I | GGTCTC | 3 cut(s) | 1481, 1731, 1890 |
| BsoBI | CYCGRG | 6 cut(s) | 94, 388, 2777, 3403, 3456, 3682 |
| Bsp119I | TTCGAA | 2 cut(s) | 236, 2885 |
| Bsp1286I | GDGCHC | 7 cut(s) | 28, 42, 178, 1537, 3638, 3806, 3937 |
| Bsp1407I | TGTACA | 1 cut(s) | 1165 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2925 |
| Bsp19I | CCATGG | 1 cut(s) | 3052 |
| BspANI | GGCC | 4 cut(s) | 76, 3015, 3680, 3878 |
| BspFNI | CGCG | 2 cut(s) | 114, 116 |
| BspHI | TCATGA | 1 cut(s) | 2512 |
| BspLI | GGNNCC | 8 cut(s) | 136, 163, 418, 763, 1534, 2131, 3133, 3429 |
| BspMAI | CTGCAG | 3 cut(s) | 1762, 1921, 3505 |
| BspMI | ACCTGC | 1 cut(s) | 3538 |
| BspOI | GCTAGC | 1 cut(s) | 1413 |
| BspT104I | TTCGAA | 2 cut(s) | 236, 2885 |
| BspT107I | GGYRCC | 4 cut(s) | 161, 416, 761, 3427 |
| BspTNI | GGTCTC | 3 cut(s) | 1481, 1731, 1890 |
| BsrDI | GCAATG | 5 cut(s) | 703, 2965, 3111, 3612, 3777 |
| BsrFI | RCCGGY | 2 cut(s) | 2987, 3874 |
| BsrGI | TGTACA | 1 cut(s) | 1165 |
| BssAI | RCCGGY | 2 cut(s) | 2987, 3874 |
| BssHII | GCGCGC | 1 cut(s) | 114 |
| BssNI | GRCGYC | 1 cut(s) | 162 |
| BssSI | CACGAG | 1 cut(s) | 41 |
| BssT1I | CCWWGG | 2 cut(s) | 3052, 3618 |
| Bst2BI | CACGAG | 1 cut(s) | 41 |
| Bst2UI | CCWGG | 3 cut(s) | 581, 3069, 3931 |
| Bst6I | CTCTTC | 5 cut(s) | 1140, 1390, 3032, 3600, 3902 |
| BstACI | GRCGYC | 1 cut(s) | 162 |
| BstAUI | TGTACA | 1 cut(s) | 1165 |
| BstBAI | YACGTR | 1 cut(s) | 2435 |
| BstBI | TTCGAA | 2 cut(s) | 236, 2885 |
| BstC8I | GCNNGC | 7 cut(s) | 24, 116, 160, 1411, 1439, 2956, 3876 |
| BstDSI | CCRYGG | 1 cut(s) | 3052 |
| BstF5I | GGATG | 6 cut(s) | 388, 814, 2332, 2638, 2847, 3472 |
| BstFNI | CGCG | 2 cut(s) | 114, 116 |
| BstH2I | RGCGCY | 1 cut(s) | 165 |
| BstHHI | GCGC | 4 cut(s) | 116, 118, 164, 3953 |
| BstMAI | GTCTC | 7 cut(s) | 193, 1387, 1481, 1731, 1890, 2106, 3845 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 743, 1180, 1652, 2027, 2993, 3331 |
| BstNI | CCWGG | 3 cut(s) | 581, 3069, 3931 |
| BstNSI | RCATGY | 3 cut(s) | 1043, 1172, 1199 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1975 |
| BstSCI | CCNGG | 7 cut(s) | 229, 387, 388, 579, 3067, 3586, 3929 |
| BstSFI | CTRYAG | 8 cut(s) | 1758, 1785, 1917, 1944, 2116, 2160, 2565, 3501 |
| BstSLI | GKGCMC | 3 cut(s) | 42, 178, 3937 |
| BstUI | CGCG | 2 cut(s) | 114, 116 |
| BstV2I | GAAGAC | 3 cut(s) | 477, 536, 2775 |
| BstX2I | RGATCY | 8 cut(s) | 13, 217, 290, 1423, 2365, 2561, 3281, 3926 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 455, 1682, 2057, 2602 |
| BstYI | RGATCY | 8 cut(s) | 13, 217, 290, 1423, 2365, 2561, 3281, 3926 |
| BsuRI | GGCC | 4 cut(s) | 76, 3015, 3680, 3878 |
| BtgI | CCRYGG | 1 cut(s) | 3052 |
| BtsCI | GGATG | 6 cut(s) | 388, 814, 2332, 2638, 2847, 3472 |
| BtsI | GCAGTG | 1 cut(s) | 1381 |
| BtsIMutI | CAGTG | 9 cut(s) | 765, 1381, 1654, 2242, 2866, 3636, 3704, 3784, 3804 |
| BveI | ACCTGC | 1 cut(s) | 3538 |
| Cac8I | GCNNGC | 7 cut(s) | 24, 116, 160, 1411, 1439, 2956, 3876 |
| CaiI | CAGNNNCTG | 4 cut(s) | 326, 1547, 1952, 2369 |
| CciI | TCATGA | 1 cut(s) | 2512 |
| CfoI | GCGC | 4 cut(s) | 116, 118, 164, 3953 |
| Cfr10I | RCCGGY | 2 cut(s) | 2987, 3874 |
| Cfr13I | GGNCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| Cfr9I | CCCGGG | 1 cut(s) | 388 |
| CseI | GACGC | 1 cut(s) | 120 |
| Csp6I | GTAC | 3 cut(s) | 1166, 2166, 3506 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 3219, 3254, 3530, 3565 |
| CviQI | GTAC | 3 cut(s) | 1166, 2166, 3506 |
| DinI | GGCGCC | 1 cut(s) | 163 |
| DraI | TTTAAA | 1 cut(s) | 1507 |
| DraIII | CACNNNGTG | 1 cut(s) | 55 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2779 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2779 |
| EaeI | YGGCCR | 2 cut(s) | 3013, 3876 |
| Eam1104I | CTCTTC | 5 cut(s) | 1140, 1390, 3032, 3600, 3902 |
| EarI | CTCTTC | 5 cut(s) | 1140, 1390, 3032, 3600, 3902 |
| EciI | GGCGGA | 1 cut(s) | 291 |
| Ecl136II | GAGCTC | 1 cut(s) | 3804 |
| Eco130I | CCWWGG | 2 cut(s) | 3052, 3618 |
| Eco147I | AGGCCT | 1 cut(s) | 3680 |
| Eco24I | GRGCYC | 3 cut(s) | 28, 1537, 3806 |
| Eco31I | GGTCTC | 3 cut(s) | 1481, 1731, 1890 |
| Eco32I | GATATC | 1 cut(s) | 3884 |
| Eco47I | GGWCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| Eco53kI | GAGCTC | 1 cut(s) | 3804 |
| Eco88I | CYCGRG | 6 cut(s) | 94, 388, 2777, 3403, 3456, 3682 |
| EcoICRI | GAGCTC | 1 cut(s) | 3804 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2129 |
| EcoRI | GAATTC | 1 cut(s) | 2887 |
| EcoRII | CCWGG | 3 cut(s) | 579, 3067, 3929 |
| EcoRV | GATATC | 1 cut(s) | 3884 |
| EcoT14I | CCWWGG | 2 cut(s) | 3052, 3618 |
| EcoT22I | ATGCAT | 5 cut(s) | 514, 739, 996, 1398, 2536 |
| EcoT38I | GRGCYC | 3 cut(s) | 28, 1537, 3806 |
| EgeI | GGCGCC | 1 cut(s) | 163 |
| EheI | GGCGCC | 1 cut(s) | 163 |
| ErhI | CCWWGG | 2 cut(s) | 3052, 3618 |
| FaqI | GGGAC | 1 cut(s) | 652 |
| FauI | CCCGC | 3 cut(s) | 62, 2140, 2638 |
| FbaI | TGATCA | 4 cut(s) | 802, 2182, 2509, 3469 |
| FokI | GGATG | 6 cut(s) | 395, 821, 2339, 2625, 2854, 3479 |
| FriOI | GRGCYC | 3 cut(s) | 28, 1537, 3806 |
| FspBI | CTAG | 5 cut(s) | 102, 842, 1410, 2838, 2892 |
| FspI | TGCGCA | 1 cut(s) | 3952 |
| GlaI | GCGC | 4 cut(s) | 115, 117, 163, 3952 |
| GsaI | CCCAGC | 1 cut(s) | 3003 |
| GsuI | CTGGAG | 4 cut(s) | 387, 1755, 1914, 2130 |
| HaeII | RGCGCY | 1 cut(s) | 165 |
| HaeIII | GGCC | 4 cut(s) | 76, 3015, 3680, 3878 |
| HapII | CCGG | 7 cut(s) | 231, 389, 1421, 1842, 2988, 3587, 3875 |
| HgaI | GACGC | 1 cut(s) | 120 |
| HhaI | GCGC | 4 cut(s) | 116, 118, 164, 3953 |
| Hin1I | GRCGYC | 1 cut(s) | 162 |
| Hin6I | GCGC | 4 cut(s) | 114, 116, 162, 3951 |
| HinP1I | GCGC | 4 cut(s) | 114, 116, 162, 3951 |
| HincII | GTYRAC | 1 cut(s) | 577 |
| HindII | GTYRAC | 1 cut(s) | 577 |
| HpaII | CCGG | 7 cut(s) | 231, 389, 1421, 1842, 2988, 3587, 3875 |
| Hpy166II | GTNNAC | 7 cut(s) | 176, 577, 704, 1586, 1636, 2011, 3241 |
| Hpy8I | GTNNAC | 7 cut(s) | 176, 577, 704, 1586, 1636, 2011, 3241 |
| HpyCH4IV | ACGT | 1 cut(s) | 2434 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 743, 1180, 1652, 2027, 2993, 3331 |
| HpySE526I | ACGT | 1 cut(s) | 2434 |
| Hsp92I | GRCGYC | 1 cut(s) | 162 |
| HspAI | GCGC | 4 cut(s) | 114, 116, 162, 3951 |
| KasI | GGCGCC | 1 cut(s) | 161 |
| KroI | GCCGGC | 1 cut(s) | 3874 |
| KroNI | GCCGGC | 1 cut(s) | 3876 |
| Ksp22I | TGATCA | 4 cut(s) | 802, 2182, 2509, 3469 |
| MaeI | CTAG | 5 cut(s) | 102, 842, 1410, 2838, 2892 |
| MaeII | ACGT | 1 cut(s) | 2434 |
| MfeI | CAATTG | 1 cut(s) | 3231 |
| MflI | RGATCY | 8 cut(s) | 13, 217, 290, 1423, 2365, 2561, 3281, 3926 |
| MhlI | GDGCHC | 7 cut(s) | 28, 42, 178, 1537, 3638, 3806, 3937 |
| MlsI | TGGCCA | 1 cut(s) | 3015 |
| MluNI | TGGCCA | 1 cut(s) | 3015 |
| Mly113I | GGCGCC | 1 cut(s) | 162 |
| MmeI | TCCRAC | 1 cut(s) | 2649 |
| Mox20I | TGGCCA | 1 cut(s) | 3015 |
| Mph1103I | ATGCAT | 5 cut(s) | 514, 739, 996, 1398, 2536 |
| MroNI | GCCGGC | 1 cut(s) | 3874 |
| MroXI | GAANNNNTTC | 3 cut(s) | 954, 1346, 2751 |
| MscI | TGGCCA | 1 cut(s) | 3015 |
| MseI | TTAA | 9 cut(s) | 297, 566, 714, 750, 791, 1449, 1464, 1506, 2415 |
| MslI | CAYNNNNRTG | 6 cut(s) | 96, 210, 453, 978, 1196, 1365 |
| Msp20I | TGGCCA | 1 cut(s) | 3015 |
| MspA1I | CMGCKG | 2 cut(s) | 2180, 2645 |
| MspI | CCGG | 7 cut(s) | 231, 389, 1421, 1842, 2988, 3587, 3875 |
| MspR9I | CCNGG | 7 cut(s) | 231, 389, 390, 581, 3069, 3588, 3931 |
| MunI | CAATTG | 1 cut(s) | 3231 |
| Mva1269I | GAATGC | 3 cut(s) | 439, 1156, 3334 |
| MvaI | CCWGG | 3 cut(s) | 581, 3069, 3931 |
| MvnI | CGCG | 2 cut(s) | 114, 116 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 743, 1180, 1652, 2027, 2993, 3331 |
| NaeI | GCCGGC | 1 cut(s) | 3876 |
| NarI | GGCGCC | 1 cut(s) | 162 |
| NciI | CCSGG | 4 cut(s) | 231, 389, 390, 3588 |
| NcoI | CCATGG | 1 cut(s) | 3052 |
| NgoMIV | GCCGGC | 1 cut(s) | 3874 |
| NheI | GCTAGC | 1 cut(s) | 1409 |
| NlaIV | GGNNCC | 8 cut(s) | 136, 163, 418, 763, 1534, 2131, 3133, 3429 |
| NmeAIII | GCCGAG | 2 cut(s) | 190, 1407 |
| NsbI | TGCGCA | 1 cut(s) | 3952 |
| NsiI | ATGCAT | 5 cut(s) | 514, 739, 996, 1398, 2536 |
| NspI | RCATGY | 3 cut(s) | 1043, 1172, 1199 |
| NspV | TTCGAA | 2 cut(s) | 236, 2885 |
| OliI | CACNNNNGTG | 1 cut(s) | 96 |
| PaeR7I | CTCGAG | 1 cut(s) | 3682 |
| PagI | TCATGA | 1 cut(s) | 2512 |
| PauI | GCGCGC | 1 cut(s) | 114 |
| PceI | AGGCCT | 1 cut(s) | 3680 |
| PciI | ACATGT | 3 cut(s) | 1039, 1168, 1195 |
| PctI | GAATGC | 3 cut(s) | 439, 1156, 3334 |
| PdiI | GCCGGC | 1 cut(s) | 3876 |
| PdmI | GAANNNNTTC | 3 cut(s) | 954, 1346, 2751 |
| PluTI | GGCGCC | 1 cut(s) | 165 |
| Ppu21I | YACGTR | 1 cut(s) | 2435 |
| PpuMI | RGGWCCY | 1 cut(s) | 2129 |
| PscI | ACATGT | 3 cut(s) | 1039, 1168, 1195 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1975 |
| Psp124BI | GAGCTC | 1 cut(s) | 3806 |
| Psp5II | RGGWCCY | 1 cut(s) | 2129 |
| Psp6I | CCWGG | 3 cut(s) | 579, 3067, 3929 |
| PspFI | CCCAGC | 1 cut(s) | 2999 |
| PspGI | CCWGG | 3 cut(s) | 579, 3067, 3929 |
| PspN4I | GGNNCC | 8 cut(s) | 136, 163, 418, 763, 1534, 2131, 3133, 3429 |
| PspPI | GGNCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| PspPPI | RGGWCCY | 1 cut(s) | 2129 |
| PspXI | VCTCGAGB | 1 cut(s) | 3682 |
| PstI | CTGCAG | 3 cut(s) | 1762, 1921, 3505 |
| PstNI | CAGNNNCTG | 4 cut(s) | 326, 1547, 1952, 2369 |
| PsuI | RGATCY | 8 cut(s) | 13, 217, 290, 1423, 2365, 2561, 3281, 3926 |
| PteI | GCGCGC | 1 cut(s) | 114 |
| PvuII | CAGCTG | 1 cut(s) | 2180 |
| RsaI | GTAC | 3 cut(s) | 1167, 2167, 3507 |
| RsaNI | GTAC | 3 cut(s) | 1166, 2166, 3506 |
| RseI | CAYNNNNRTG | 6 cut(s) | 96, 210, 453, 978, 1196, 1365 |
| SacI | GAGCTC | 1 cut(s) | 3806 |
| SaqAI | TTAA | 9 cut(s) | 297, 566, 714, 750, 791, 1449, 1464, 1506, 2415 |
| Sau96I | GGNCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| ScrFI | CCNGG | 7 cut(s) | 231, 389, 390, 581, 3069, 3588, 3931 |
| SduI | GDGCHC | 7 cut(s) | 28, 42, 178, 1537, 3638, 3806, 3937 |
| SfcI | CTRYAG | 8 cut(s) | 1758, 1785, 1917, 1944, 2116, 2160, 2565, 3501 |
| SfoI | GGCGCC | 1 cut(s) | 163 |
| Sfr274I | CTCGAG | 1 cut(s) | 3682 |
| SfuI | TTCGAA | 2 cut(s) | 236, 2885 |
| SinI | GGWCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| SlaI | CTCGAG | 1 cut(s) | 3682 |
| SmaI | CCCGGG | 1 cut(s) | 390 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 96, 210, 453, 978, 1196, 1365 |
| SmlI | CTYRAG | 2 cut(s) | 2631, 3682 |
| SmoI | CTYRAG | 2 cut(s) | 2631, 3682 |
| SseBI | AGGCCT | 1 cut(s) | 3680 |
| SspDI | GGCGCC | 1 cut(s) | 161 |
| SspI | AATATT | 1 cut(s) | 2752 |
| SspMI | CTAG | 5 cut(s) | 102, 842, 1410, 2838, 2892 |
| SstI | GAGCTC | 1 cut(s) | 3806 |
| StuI | AGGCCT | 1 cut(s) | 3680 |
| StyD4I | CCNGG | 7 cut(s) | 229, 387, 388, 579, 3067, 3586, 3929 |
| StyI | CCWWGG | 2 cut(s) | 3052, 3618 |
| TaiI | ACGT | 1 cut(s) | 2437 |
| TaqI | TCGA | 4 cut(s) | 236, 2885, 3683, 3806 |
| TaqII | GACCGA | 2 cut(s) | 548, 1505 |
| TatI | WGTACW | 2 cut(s) | 1165, 3505 |
| TauI | GCSGC | 1 cut(s) | 1374 |
| Tru1I | TTAA | 9 cut(s) | 297, 566, 714, 750, 791, 1449, 1464, 1506, 2415 |
| Tru9I | TTAA | 9 cut(s) | 297, 566, 714, 750, 791, 1449, 1464, 1506, 2415 |
| TscAI | CASTG | 9 cut(s) | 772, 1381, 1654, 2242, 2866, 3643, 3711, 3784, 3804 |
| TspGWI | ACGGA | 2 cut(s) | 432, 3711 |
| TspMI | CCCGGG | 1 cut(s) | 388 |
| TspRI | CASTG | 9 cut(s) | 772, 1381, 1654, 2242, 2866, 3643, 3711, 3784, 3804 |
| VneI | GTGCAC | 1 cut(s) | 174 |
| VpaK11BI | GGWCC | 6 cut(s) | 1685, 2060, 2129, 3049, 3142, 3782 |
| XapI | RAATTY | 5 cut(s) | 2199, 2353, 2702, 2887, 2910 |
| XbaI | TCTAGA | 1 cut(s) | 841 |
| XceI | RCATGY | 3 cut(s) | 1043, 1172, 1199 |
| XhoI | CTCGAG | 1 cut(s) | 3682 |
| XmaI | CCCGGG | 1 cut(s) | 388 |
| XmnI | GAANNNNTTC | 3 cut(s) | 954, 1346, 2751 |
| XspI | CTAG | 5 cut(s) | 102, 842, 1410, 2838, 2892 |
| Zsp2I | ATGCAT | 5 cut(s) | 514, 739, 996, 1398, 2536 |