Rw2G026540

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001352
Physical Location & Seq
Forward (+)
40988886 .. 40991935
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G026540.1

Sequence Viewer

Length: 1170 bp
ATGGAGGGCAAACACCTGTTTTTGAAGCTGGCACAGTACCGGGTGCGGGTCAATCTTAACGATTCTGATCGGCAAAAGGCTGAGGAACGTACCCACCTCTTCATCAAAAGATTCCCTGAGTGGCAACGGAAAGCGATAATCCTTGGCATCGTCAGGACTACGAGAAGCCTCCTTAGACCTAGGTATATAGATGAGGAGCTTATTCGAAAATTTATTGAAAAAATGAAGGAGTGGAAGAAGCTGGTGGTGAAGGATGATGATTTCGAGATGATGTCGGGGGATGAGGAGGAGGAGGAGGAGGAGGGGGAAGAAGAACCGACAGAACAAGACAGGAGGGTGTGGTCGTACCTTTCCAGTAGCCTGGGATTCAGAATGAAGCCAGGAGAAGATGTCAAGATCGTCAGGCTGATGATTCCTTTCTTAGCCCAATGTTGTGGACGTCTTCCACCAGAAGGACCCGCGAGATGTGTCCACATCAAAATGTTCATACACCAGTTCCAAACATATATCATTAAATATTGCCTTAGCGACCTGAACGACGTGGAGATGCTGCTCGATGTCATTGCGGCTGTCGAGTCAAGTCCAGCAAATCCAGAAAATAAGTTAAAGAAAAATATACCAACTAAAGAAGAAGGGATTGCTGCCCCCATGTACATCTTAAAACTAGAGAGAAGAGAATATGGAGACCACAGCAGAAAAGGACCTGCAGAAGAAAATCTTGTGGCAAATCCTATAGCTATTGTTCAATCTGCTGAAGGACAGGAATCTTATGAGAAGTCCAATGAGTCTTTACAGGTGAACTTTGCTGCCAGTGATGCTGGTATAGATCTAGCTACCCACTCAGTTGGTCTGGATGATAATGAAAAAGATGGAAACTGTGATGGGAGTGGGGAGTTTGGAGACTACAGCAGAAAAGGACCTGCAGAAGAAAATCTTGTGGCAACTCCTATAGCTACTGTTCACTCAGCTGATCATCATGAAAACCAAATTTATCCAGCAGCCAACTCACACAAAGGTGGTGATGCCGGTGATTATGAGAAAGGGGTAATTGAAAAGTGTAATAAAATTGGAGATGCCGGTTTCAATGAAAAGGCTGAAACCGCTAACTTATGTGGGGATGACAAAAGCAAAGGCGTAATTGAAAAGTGTCGGTTGCTGCAACGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

389

Amino Acids

44.0

Weight (kDa)

5.26

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 442
Acc36I ACCTGC 2 cut(s) 712, 928
AccII CGCG 1 cut(s) 461
AciI CCGC 4 cut(s) 46, 459, 566, 1101
AclI AACGTT 1 cut(s) 1162
AcsI RAATTY 2 cut(s) 209, 987
AcuI CTGAAG 1 cut(s) 774
AcyI GRCGYC 1 cut(s) 439
AfaI GTAC 4 cut(s) 38, 91, 347, 653
AfiI CCNNNNNNNGG 2 cut(s) 46, 452
AgsI TTSAA 6 cut(s) 25, 218, 746, 1052, 1084, 1142
AjiI CACGTC 1 cut(s) 541
AjnI CCWGG 2 cut(s) 360, 379
AleI CACNNNNGTG 1 cut(s) 1014
AluBI AGCT 7 cut(s) 28, 199, 241, 737, 833, 953, 968
AluI AGCT 7 cut(s) 28, 199, 241, 737, 833, 953, 968
Alw26I GTCTC 2 cut(s) 678, 894
ApeKI GCWGC 5 cut(s) 550, 641, 806, 998, 1156
ApoI RAATTY 2 cut(s) 209, 987
AspA2I CCTAGG 1 cut(s) 179
AspS9I GGNCC 3 cut(s) 455, 701, 917
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 4 cut(s) 259, 808, 1031, 1040
AsuII TTCGAA 1 cut(s) 205
AvaII GGWCC 3 cut(s) 455, 701, 917
AvrII CCTAGG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 5 cut(s) 537, 628, 793, 1010, 1143
BccI CCATC 2 cut(s) 863, 875
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 2 cut(s) 362, 381
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 678, 894
BfaI CTAG 3 cut(s) 180, 665, 830
BfmI CTRYAG 5 cut(s) 705, 732, 904, 921, 948
BfuAI ACCTGC 2 cut(s) 712, 928
BglII AGATCT 1 cut(s) 826
BisI GCNGC 6 cut(s) 551, 567, 642, 807, 999, 1157
BlnI CCTAGG 1 cut(s) 179
BlsI GCNGC 6 cut(s) 552, 568, 643, 808, 1000, 1158
Bme1390I CCNGG 3 cut(s) 41, 362, 381
Bme18I GGWCC 3 cut(s) 455, 701, 917
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 3 cut(s) 455, 701, 917
BmiI GGNNCC 1 cut(s) 457
BmrFI CCNGG 3 cut(s) 41, 362, 381
BmsI GCATC 5 cut(s) 156, 537, 805, 1012, 1063
BpiI GAAGAC 1 cut(s) 434
Bpu10I CCTNAGC 2 cut(s) 81, 524
Bpu14I TTCGAA 1 cut(s) 205
BpuMI CCSGG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 439
BsaI GGTCTC 1 cut(s) 678
BsaJI CCNNGG 3 cut(s) 142, 179, 361
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 452
Bse118I RCCGGY 2 cut(s) 1025, 1076
Bse1I ACTGG 3 cut(s) 354, 493, 810
Bse3DI GCAATG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 2 cut(s) 362, 381
BseDI CCNNGG 3 cut(s) 142, 179, 361
BseGI GGATG 4 cut(s) 259, 286, 859, 1123
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 46, 452
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 4 cut(s) 72, 108, 855, 978
BseNI ACTGG 3 cut(s) 354, 493, 810
BseRI GAGGAG 7 cut(s) 209, 299, 302, 305, 308, 311, 314
BseXI GCAGC 5 cut(s) 537, 628, 793, 1010, 1143
Bsh1236I CGCG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 40, 1026, 1077
BslI CCNNNNNNNGG 2 cut(s) 46, 452
BsmAI GTCTC 2 cut(s) 678, 894
Bso31I GGTCTC 1 cut(s) 678
Bsp119I TTCGAA 1 cut(s) 205
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 4 cut(s) 67, 396, 826, 970
BspACI CCGC 4 cut(s) 46, 459, 566, 1101
BspCNI CTCAG 4 cut(s) 73, 109, 854, 977
BspFNI CGCG 1 cut(s) 461
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 1 cut(s) 457
BspMAI CTGCAG 2 cut(s) 709, 925
BspMI ACCTGC 2 cut(s) 712, 928
BspT104I TTCGAA 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 2 cut(s) 1025, 1076
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 3 cut(s) 354, 493, 810
BssAI RCCGGY 2 cut(s) 1025, 1076
BssECI CCNNGG 3 cut(s) 142, 179, 361
BssMI GATC 4 cut(s) 67, 396, 826, 970
BssNI GRCGYC 1 cut(s) 439
BssT1I CCWWGG 2 cut(s) 142, 179
Bst2UI CCWGG 2 cut(s) 362, 381
Bst4CI ACNGT 3 cut(s) 36, 878, 958
Bst6I CTCTTC 2 cut(s) 104, 667
BstACI GRCGYC 1 cut(s) 439
BstAUI TGTACA 1 cut(s) 651
BstBI TTCGAA 1 cut(s) 205
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 8 cut(s) 81, 117, 173, 421, 524, 841, 964, 1167
BstF5I GGATG 4 cut(s) 259, 286, 859, 1123
BstFNI CGCG 1 cut(s) 461
BstKTI GATC 4 cut(s) 70, 399, 829, 973
BstMAI GTCTC 2 cut(s) 678, 894
BstMBI GATC 4 cut(s) 67, 396, 826, 970
BstMWI GCNNNNNNNGC 2 cut(s) 815, 1100
BstNI CCWGG 2 cut(s) 362, 381
BstSCI CCNGG 3 cut(s) 39, 360, 379
BstSFI CTRYAG 5 cut(s) 705, 732, 904, 921, 948
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 5 cut(s) 537, 628, 793, 1010, 1143
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 826
BstXI CCANNNNNNTGG 3 cut(s) 361, 434, 845
BstYI RGATCY 1 cut(s) 826
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 4 cut(s) 259, 286, 859, 1123
BtsIMutI CAGTG 1 cut(s) 817
BveI ACCTGC 2 cut(s) 712, 928
Cac8I GCNNGC 1 cut(s) 30
CciI TCATGA 1 cut(s) 976
Cfr10I RCCGGY 2 cut(s) 1025, 1076
Cfr13I GGNCC 3 cut(s) 455, 701, 917
Csp6I GTAC 4 cut(s) 37, 90, 346, 652
CviAII CATG 2 cut(s) 649, 977
CviQI GTAC 4 cut(s) 37, 90, 346, 652
DdeI CTNAG 8 cut(s) 81, 117, 173, 421, 524, 841, 964, 1167
DpnI GATC 4 cut(s) 69, 398, 828, 972
DpnII GATC 4 cut(s) 67, 396, 826, 970
Eam1104I CTCTTC 2 cut(s) 104, 667
EarI CTCTTC 2 cut(s) 104, 667
Eco130I CCWWGG 2 cut(s) 142, 179
Eco31I GGTCTC 1 cut(s) 678
Eco47I GGWCC 3 cut(s) 455, 701, 917
Eco57I CTGAAG 1 cut(s) 774
EcoO109I RGGNCCY 3 cut(s) 455, 701, 917
EcoRII CCWGG 2 cut(s) 360, 379
EcoT14I CCWWGG 2 cut(s) 142, 179
ErhI CCWWGG 2 cut(s) 142, 179
FaeI CATG 2 cut(s) 652, 980
FalI AAGNNNNNCTT 4 cut(s) 702, 734, 918, 950
FatI CATG 2 cut(s) 648, 976
FauI CCCGC 2 cut(s) 39, 466
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 6 cut(s) 551, 567, 642, 807, 999, 1157
FokI GGATG 4 cut(s) 266, 293, 866, 1130
Fsp4HI GCNGC 6 cut(s) 551, 567, 642, 807, 999, 1157
FspBI CTAG 3 cut(s) 180, 665, 830
GluI GCNGC 6 cut(s) 551, 567, 642, 807, 999, 1157
HapII CCGG 3 cut(s) 40, 1026, 1077
Hin1I GRCGYC 1 cut(s) 439
Hin1II CATG 2 cut(s) 652, 980
HinfI GANTC 7 cut(s) 62, 111, 366, 412, 575, 764, 785
HpaII CCGG 3 cut(s) 40, 1026, 1077
HphI GGTGA 4 cut(s) 259, 808, 1031, 1040
Hpy166II GTNNAC 4 cut(s) 437, 472, 799, 961
Hpy188I TCNGA 2 cut(s) 67, 371
Hpy188III TCNNGA 6 cut(s) 154, 265, 394, 593, 851, 977
Hpy8I GTNNAC 4 cut(s) 437, 472, 799, 961
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 5 cut(s) 220, 244, 446, 626, 749
HpyCH4III ACNGT 3 cut(s) 36, 878, 958
HpyCH4IV ACGT 4 cut(s) 88, 439, 540, 1162
HpyCH4V TGCA 3 cut(s) 707, 923, 1159
HpyF10VI GCNNNNNNNGC 2 cut(s) 815, 1100
HpyF3I CTNAG 8 cut(s) 81, 117, 173, 421, 524, 841, 964, 1167
HpySE526I ACGT 4 cut(s) 88, 439, 540, 1162
Hsp92I GRCGYC 1 cut(s) 439
Hsp92II CATG 2 cut(s) 652, 980
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 4 cut(s) 67, 396, 826, 970
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 5 cut(s) 537, 628, 793, 1010, 1143
LweI GCATC 5 cut(s) 156, 537, 805, 1012, 1063
MaeI CTAG 3 cut(s) 180, 665, 830
MaeII ACGT 4 cut(s) 88, 439, 540, 1162
MalI GATC 4 cut(s) 69, 398, 828, 972
MboI GATC 4 cut(s) 67, 396, 826, 970
MflI RGATCY 1 cut(s) 826
MluCI AATT 5 cut(s) 209, 987, 1047, 1065, 1137
MlyI GAGTC 2 cut(s) 584, 794
MseI TTAA 4 cut(s) 57, 513, 605, 659
MslI CAYNNNNRTG 2 cut(s) 479, 1014
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 3 cut(s) 40, 1026, 1077
MspR9I CCNGG 3 cut(s) 41, 362, 381
MvaI CCWGG 2 cut(s) 362, 381
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 815, 1100
NciI CCSGG 1 cut(s) 41
NdeII GATC 4 cut(s) 67, 396, 826, 970
NlaIII CATG 2 cut(s) 652, 980
NlaIV GGNNCC 1 cut(s) 457
NspV TTCGAA 1 cut(s) 205
OliI CACNNNNGTG 1 cut(s) 1014
PagI TCATGA 1 cut(s) 976
PfeI GAWTC 5 cut(s) 62, 111, 366, 412, 764
PkrI GCNGC 6 cut(s) 552, 568, 643, 808, 1000, 1158
PleI GAGTC 2 cut(s) 583, 793
PpsI GAGTC 2 cut(s) 583, 793
PpuMI RGGWCCY 3 cut(s) 455, 701, 917
Psp1406I AACGTT 1 cut(s) 1162
Psp5II RGGWCCY 3 cut(s) 455, 701, 917
Psp6I CCWGG 2 cut(s) 360, 379
PspGI CCWGG 2 cut(s) 360, 379
PspN4I GGNNCC 1 cut(s) 457
PspPI GGNCC 3 cut(s) 455, 701, 917
PspPPI RGGWCCY 3 cut(s) 455, 701, 917
PstI CTGCAG 2 cut(s) 709, 925
PsuI RGATCY 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 968
RsaI GTAC 4 cut(s) 38, 91, 347, 653
RsaNI GTAC 4 cut(s) 37, 90, 346, 652
RseI CAYNNNNRTG 2 cut(s) 479, 1014
SaqAI TTAA 4 cut(s) 57, 513, 605, 659
SatI GCNGC 6 cut(s) 551, 567, 642, 807, 999, 1157
Sau3AI GATC 4 cut(s) 67, 396, 826, 970
Sau96I GGNCC 3 cut(s) 455, 701, 917
SchI GAGTC 2 cut(s) 584, 794
ScrFI CCNGG 3 cut(s) 41, 362, 381
SfaNI GCATC 5 cut(s) 156, 537, 805, 1012, 1063
SfcI CTRYAG 5 cut(s) 705, 732, 904, 921, 948
SfuI TTCGAA 1 cut(s) 205
SinI GGWCC 3 cut(s) 455, 701, 917
SmiMI CAYNNNNRTG 2 cut(s) 479, 1014
Sse9I AATT 5 cut(s) 209, 987, 1047, 1065, 1137
SsiI CCGC 4 cut(s) 46, 459, 566, 1101
SspI AATATT 1 cut(s) 518
SspMI CTAG 3 cut(s) 180, 665, 830
StyD4I CCNGG 3 cut(s) 39, 360, 379
StyI CCWWGG 2 cut(s) 142, 179
TaaI ACNGT 3 cut(s) 36, 878, 958
TaiI ACGT 4 cut(s) 91, 442, 543, 1165
TaqI TCGA 4 cut(s) 205, 264, 555, 573
TasI AATT 5 cut(s) 209, 987, 1047, 1065, 1137
TatI WGTACW 1 cut(s) 651
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 5 cut(s) 62, 111, 366, 412, 764
Tru1I TTAA 4 cut(s) 57, 513, 605, 659
Tru9I TTAA 4 cut(s) 57, 513, 605, 659
TscAI CASTG 1 cut(s) 817
TseI GCWGC 5 cut(s) 550, 641, 806, 998, 1156
TspDTI ATGAA 7 cut(s) 91, 239, 389, 475, 876, 993, 1101
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 1 cut(s) 817
VpaK11BI GGWCC 3 cut(s) 455, 701, 917
XapI RAATTY 2 cut(s) 209, 987
XmaJI CCTAGG 1 cut(s) 179
XspI CTAG 3 cut(s) 180, 665, 830
ZraI GACGTC 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.