Rmu_sc0006593.1_g000007

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006593.1
OG0001352
Physical Location & Seq
Reverse (-)
37819 .. 43135
5317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006593.1_g000007.1.cds

Sequence Viewer

Length: 558 bp
atgaagaagccgaagaaaccagcggtgaaggaggatgaattcgagatgatgttgggggatgaggaggaggaggagacgctgttggaggagggggaagaagaaccgacagaagaagacaggaaggtgtggttggtactttgcaggagactgggattccttatgaaaccaggagaagatgccaacgacctgaacgacgtggggatgttgctcgacgtcattgaggctgtcaagtccagtccagcaaatccagaaaataaggtgaactttgctgccagtgatgctggtatagatctagctacccactcagttggtccggatgataatgaaaaagatggaaactgtgatgggagtggcgagtctggagactacagaagaaaaggacccacagaagaaaatcttgtggcaactcctatagctactgttcaatcagctggtcatcaaaatccagcagctaacttacacaaagatgatgatgccggtgattatgagaagggcgtaattgaaaagtgtaataaaattggagatgctggttgcaatgaaaaggctgaaacctcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.06

Weight (kDa)

4.35

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 135
AgsI TTSAA 2 cut(s) 425, 503
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 296, 416, 431, 452
AluI AGCT 4 cut(s) 296, 416, 431, 452
Alw26I GTCTC 3 cut(s) 68, 139, 357
Aor13HI TCCGGA 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 269, 449
ApoI RAATTY 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 311, 380
AsuHPI GGTGA 3 cut(s) 37, 271, 491
AvaII GGWCC 2 cut(s) 311, 380
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 256, 461
BccI CCATC 2 cut(s) 326, 338
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 3 cut(s) 68, 139, 357
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 2 cut(s) 367, 411
BglII AGATCT 1 cut(s) 289
BisI GCNGC 2 cut(s) 270, 450
BlsI GCNGC 2 cut(s) 271, 451
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 311, 380
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 311, 380
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 168
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 4 cut(s) 166, 268, 463, 514
BmuI ACTGGG 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 313
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 3 cut(s) 153, 234, 273
Bse3DI GCAATG 1 cut(s) 541
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 1 cut(s) 168
BseGI GGATG 4 cut(s) 40, 64, 207, 322
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 3 cut(s) 153, 234, 273
BseRI GAGGAG 5 cut(s) 77, 80, 83, 86, 101
BseXI GCAGC 2 cut(s) 256, 461
BsiSI CCGG 2 cut(s) 314, 477
BsmAI GTCTC 3 cut(s) 68, 139, 357
BsmBI CGTCTC 1 cut(s) 68
Bsp13I TCCGGA 1 cut(s) 313
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 317
BspEI TCCGGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 541
BsrFI RCCGGY 1 cut(s) 476
BsrI ACTGG 3 cut(s) 153, 234, 273
BssAI RCCGGY 1 cut(s) 476
BssMI GATC 1 cut(s) 289
BssNI GRCGYC 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 341, 421
BstACI GRCGYC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 304
BstF5I GGATG 4 cut(s) 40, 64, 207, 322
BstKTI GATC 1 cut(s) 292
BstMAI GTCTC 3 cut(s) 68, 139, 357
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 367, 411
BstV1I GCAGC 2 cut(s) 256, 461
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 289
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 1 cut(s) 289
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 4 cut(s) 40, 64, 207, 322
BtsIMutI CAGTG 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 2 cut(s) 311, 380
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 134
CviJI RGCY 7 cut(s) 10, 224, 296, 416, 431, 452, 545
CviKI_1 RGCY 7 cut(s) 10, 224, 296, 416, 431, 452, 545
CviQI GTAC 1 cut(s) 134
DdeI CTNAG 1 cut(s) 304
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 311, 380
EcoO109I RGGNCCY 1 cut(s) 380
EcoRI GAATTC 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 68
FaiI YATR 4 cut(s) 161, 287, 413, 486
FalI AAGNNNNNCTT 4 cut(s) 248, 280, 381, 413
Fnu4HI GCNGC 2 cut(s) 270, 450
FokI GGATG 4 cut(s) 47, 71, 214, 329
Fsp4HI GCNGC 2 cut(s) 270, 450
FspBI CTAG 1 cut(s) 293
GluI GCNGC 2 cut(s) 270, 450
GsuI CTGGAG 1 cut(s) 381
HapII CCGG 2 cut(s) 314, 477
HgaI GACGC 1 cut(s) 85
Hin1I GRCGYC 1 cut(s) 213
HinfI GANTC 2 cut(s) 153, 356
HpaII CCGG 2 cut(s) 314, 477
HphI GGTGA 3 cut(s) 37, 271, 491
Hpy166II GTNNAC 1 cut(s) 262
Hpy188III TCNNGA 4 cut(s) 43, 248, 314, 360
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 2 cut(s) 197, 215
HpyAV CCTTC 3 cut(s) 22, 115, 484
HpyCH4III ACNGT 2 cut(s) 341, 421
HpyCH4IV ACGT 2 cut(s) 195, 213
HpyCH4V TGCA 2 cut(s) 141, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 304
HpySE526I ACGT 2 cut(s) 195, 213
Hsp92I GRCGYC 1 cut(s) 213
Kpn2I TCCGGA 1 cut(s) 313
Kzo9I GATC 1 cut(s) 289
Lsp1109I GCAGC 2 cut(s) 256, 461
LweI GCATC 4 cut(s) 166, 268, 463, 514
MaeI CTAG 1 cut(s) 293
MaeII ACGT 2 cut(s) 195, 213
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 9 cut(s) 16, 25, 107, 110, 122, 125, 185, 384, 401
MflI RGATCY 1 cut(s) 289
MluCI AATT 3 cut(s) 38, 498, 516
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 8 cut(s) 25, 55, 58, 61, 64, 79, 82, 214
MroI TCCGGA 1 cut(s) 313
MseI TTAA 1 cut(s) 556
MslI CAYNNNNRTG 1 cut(s) 465
MspA1I CMGCKG 2 cut(s) 23, 431
MspI CCGG 2 cut(s) 314, 477
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeII GATC 1 cut(s) 289
NlaIV GGNNCC 1 cut(s) 382
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 1 cut(s) 153
PkrI GCNGC 2 cut(s) 271, 451
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 380
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 2 cut(s) 311, 380
PspPPI RGGWCCY 1 cut(s) 380
PsuI RGATCY 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 465
SaqAI TTAA 1 cut(s) 556
SatI GCNGC 2 cut(s) 270, 450
Sau3AI GATC 1 cut(s) 289
Sau96I GGNCC 2 cut(s) 311, 380
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 168
SfaNI GCATC 4 cut(s) 166, 268, 463, 514
SfcI CTRYAG 2 cut(s) 367, 411
SinI GGWCC 2 cut(s) 311, 380
SmiMI CAYNNNNRTG 1 cut(s) 465
Sse9I AATT 3 cut(s) 38, 498, 516
SsiI CCGC 1 cut(s) 23
SspMI CTAG 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 341, 421
TaiI ACGT 2 cut(s) 198, 216
TaqI TCGA 2 cut(s) 42, 210
TasI AATT 3 cut(s) 38, 498, 516
TfiI GAWTC 1 cut(s) 153
Tru1I TTAA 1 cut(s) 556
Tru9I TTAA 1 cut(s) 556
TscAI CASTG 1 cut(s) 280
TseI GCWGC 2 cut(s) 269, 449
TspDTI ATGAA 5 cut(s) 17, 51, 176, 339, 552
TspRI CASTG 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 311, 380
XapI RAATTY 1 cut(s) 38
XspI CTAG 1 cut(s) 293
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.