AT5G44440

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000925
Physical Location & Seq
Reverse (-)
17910480 .. 17912643
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44440.1

Sequence Viewer

Length: 1602 bp
ATGGAGTTCTCAAGCTTTTTGTTTACTATTTTACTCTTTTCACTGAATATTTCTCCTCTTGTTTCAGCTCATGGCTCCAACCATGAAGACTTTCTTAAGTGTCTTTCTTACCGAATGAACGACAACACCGTCGAACCCAAAGTCATACACACTTCCAAAGACTCTTCCTTTTTTTCCATATTGGATTCTTCGATACAAAATCCAAGATTCTCGGTTTCCGAAACACCTAAACCGGTCTCAATCATCACTCCCGTTAAAGCCAGTGACGTTCAAACTGTGATCAGATGCGCACAGCTGCATGGAATTCATGTAAGGACTCGGAGCGCCGGTCACTGCTACGAAGGTCTCTCTTACATTGCGTACAACAAACCATTCGCTGTCATCGATTTAAGGAATCTTCGGTCTATATCATTAGACGTTGATAACCGGACAGGTTGGGTCCAAACCGGAGCAACGGCTGGGGAATTGTATTATGAGATTGGGAAAACCACCAAATCTCTTGCTTTCCCGGCCGGTATTCACCCAACCGTTGGTGTAGGCGGACAATTTAGCGGTGGTGGTTATGGCACATTGCTAAGGAAATACGGTTTAGCTGCGGATAACATAATCGATGCACTTGTGGTTGACGCGAGTGGGAGGATTCTTGACCGACAAGCAATGGGCGAGGATTACTTTTGGGCTATTCGTGGAGGGGGCGGATCAAGCTTCGGTGTTATACTATCGTGGAAGGTTAAATTAGTCGATGTTCCATCCACAATTACCGTGTTTAAAGTCCAGAAAACATCGAAGAAAGAGGCCGTTAGGATCATCAAGAAATGGCAATATGCTGCAGATAAGGTTCCTGATGATCTTTTCATTAGGACAACATTGGAGAGATCAAACAAGAACGCAGTACACGCTTTGTTCACTGGACTATATATTGGTCCGGTGAACAATCTATTGGCGTTGATGGAAGAAAAGTTTCCGGAACTAGGTCTTGAGAAAGAAGGTTGTGAAGAGATGAGTTGGATTGAGTCTGTACTCTGGTTTGCTGATTTCCCTAAAGGAGAATCTCTTGGTGTTCTCACGAATCGTGAGCGTACATCTCTATCTTTCAAAGGCAAAGATGATTTTGTCCAAGAACCGATACCCGAGGCTGCAATTCAAGAGATATGGAGGCGATTAGAAGCCCCCGAGGCTCGGCTTGGAAAGATCATATTAACTCCATTTGGTGGGAAAATGAGTGAAATGGCAGAGTACGAAACACCATTCCCACATCGAGGAGGGAATCTCTATGAGATTCAGTATGTGGCTTACTGGAGAGAAGAGGAAGACAAGAACAAGACGGAGACAGATAAATATCTGAAATGGGTCGATAGCGTTTACGAATTTATGACTCCATATGTTTCGAAATCTCCAAGAGGAGCTTATGTCAATTTCAAGGATATGGATTTGGGTATGTATCTTGGAAAGAAGAAGACAAAGTACGAGGAAGGAAAGAGTTGGGGAGTGAAGTATTTCAAGAACAATTTCGAGAGATTGGTGAGAGTGAAGACTAGGGTTGATCCAACAGATTTCTTCTGCGATGAACAGAGCATTCCTCTGGTGAACAAAGTTACCTGA

Protein Analysis

533

Amino Acids

60.14

Weight (kDa)

7.6

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 81 - 215 1e-21 FAD binding domain
BBE PF08031 469 - 527 5.7e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
Acc16I TGCGCA 1 cut(s) 289
AccB7I CCANNNNNTGG 2 cut(s) 530, 1211
AccII CGCG 1 cut(s) 629
AccIII TCCGGA 1 cut(s) 964
AciI CCGC 4 cut(s) 540, 552, 596, 696
AclWI GGATC 3 cut(s) 706, 812, 1538
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 2 cut(s) 303, 1367
AfaI GTAC 6 cut(s) 362, 894, 1020, 1081, 1238, 1466
AfiI CCNNNNNNNGG 5 cut(s) 530, 971, 1179, 1211, 1259
AflII CTTAAG 1 cut(s) 95
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 272, 1096, 1145, 1420, 1501
AjuI GAANNNNNNNTTGG 2 cut(s) 923, 955
AluBI AGCT 6 cut(s) 15, 68, 295, 593, 705, 1406
AluI AGCT 6 cut(s) 15, 68, 295, 593, 705, 1406
Alw26I GTCTC 3 cut(s) 241, 350, 1322
AlwI GGATC 3 cut(s) 706, 812, 1538
Ama87I CYCGRG 2 cut(s) 1130, 1172
Aor13HI TCCGGA 1 cut(s) 964
AoxI GGCC 2 cut(s) 510, 795
ApeKI GCWGC 4 cut(s) 295, 593, 827, 1136
ApoI RAATTY 2 cut(s) 303, 1367
ArsI GACNNNNNNTTYG 2 cut(s) 126, 158
AsiGI ACCGGT 1 cut(s) 232
Asp700I GAANNNNTTC 4 cut(s) 90, 960, 1496, 1508
AspLEI GCGC 2 cut(s) 290, 326
AspS9I GGNCC 2 cut(s) 439, 923
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 4 cut(s) 512, 940, 1534, 1597
AsuII TTCGAA 1 cut(s) 1388
AvaI CYCGRG 2 cut(s) 1130, 1172
AvaII GGWCC 2 cut(s) 439, 923
BarI GAAGNNNNNNTAC 2 cut(s) 1448, 1480
BbsI GAAGAC 4 cut(s) 93, 1317, 1463, 1538
BbvI GCAGC 4 cut(s) 282, 580, 814, 1123
BccI CCATC 2 cut(s) 757, 943
BceAI ACGGC 2 cut(s) 471, 782
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 3 cut(s) 241, 350, 1322
BfaI CTAG 2 cut(s) 971, 1536
BfmI CTRYAG 1 cut(s) 828
BfoI RGCGCY 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 95
BglI GCCNNNNNGGC 1 cut(s) 1175
BisI GCNGC 4 cut(s) 296, 594, 828, 1137
BlsI GCNGC 4 cut(s) 297, 595, 829, 1138
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 2 cut(s) 439, 923
BmeT110I CYCGRG 2 cut(s) 1130, 1172
BmgT120I GGNCC 2 cut(s) 439, 923
BmiI GGNNCC 3 cut(s) 76, 440, 840
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 2 cut(s) 275, 601
BpiI GAAGAC 4 cut(s) 93, 1317, 1463, 1538
BplI GAGNNNNNCTC 4 cut(s) 1254, 1286, 1564, 1596
BpmI CTGGAG 1 cut(s) 1318
Bpu10I CCTNAGC 1 cut(s) 575
Bpu14I TTCGAA 1 cut(s) 1388
BpuEI CTTGAG 1 cut(s) 998
BpuMI CCSGG 1 cut(s) 509
Bsa29I ATCGAT 2 cut(s) 384, 609
BsaBI GATNNNNATC 1 cut(s) 1338
BsaI GGTCTC 2 cut(s) 241, 350
BsaJI CCNNGG 2 cut(s) 1131, 1173
BsaWI WCCGGW 5 cut(s) 232, 426, 446, 925, 964
BsaXI ACNNNNNCTCC 4 cut(s) 313, 343, 1395, 1425
Bsc4I CCNNNNNNNGG 5 cut(s) 530, 971, 1179, 1211, 1259
Bse118I RCCGGY 3 cut(s) 232, 326, 512
Bse1I ACTGG 3 cut(s) 261, 913, 1301
Bse3DI GCAATG 3 cut(s) 354, 569, 663
Bse8I GATNNNNATC 1 cut(s) 1338
BseAI TCCGGA 1 cut(s) 964
BseCI ATCGAT 2 cut(s) 384, 609
BseDI CCNNGG 2 cut(s) 1131, 1173
BseGI GGATG 1 cut(s) 749
BseJI GATNNNNATC 1 cut(s) 1338
BseLI CCNNNNNNNGG 5 cut(s) 530, 971, 1179, 1211, 1259
BseMI GCAATG 3 cut(s) 354, 569, 663
BseNI ACTGG 3 cut(s) 261, 913, 1301
BseRI GAGGAG 3 cut(s) 45, 1275, 1416
BseX3I CGGCCG 1 cut(s) 510
BseXI GCAGC 4 cut(s) 282, 580, 814, 1123
BseYI CCCAGC 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 629
Bsh1285I CGRYCG 1 cut(s) 513
BshFI GGCC 2 cut(s) 512, 797
BshTI ACCGGT 1 cut(s) 232
BshVI ATCGAT 2 cut(s) 384, 609
BsiEI CGRYCG 1 cut(s) 513
BsiHKCI CYCGRG 2 cut(s) 1130, 1172
BsiSI CCGG 8 cut(s) 233, 327, 427, 447, 509, 513, 926, 965
BslI CCNNNNNNNGG 5 cut(s) 530, 971, 1179, 1211, 1259
BsmAI GTCTC 3 cut(s) 241, 350, 1322
BsmI GAATGC 1 cut(s) 1575
BsnI GGCC 2 cut(s) 512, 797
Bso31I GGTCTC 2 cut(s) 241, 350
BsoBI CYCGRG 2 cut(s) 1130, 1172
Bsp119I TTCGAA 1 cut(s) 1388
Bsp13I TCCGGA 1 cut(s) 964
Bsp143I GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
BspACI CCGC 4 cut(s) 540, 552, 596, 696
BspANI GGCC 2 cut(s) 512, 797
BspDI ATCGAT 2 cut(s) 384, 609
BspEI TCCGGA 1 cut(s) 964
BspFNI CGCG 1 cut(s) 629
BspLI GGNNCC 3 cut(s) 76, 440, 840
BspMAI CTGCAG 1 cut(s) 832
BspPI GGATC 3 cut(s) 706, 812, 1538
BspT104I TTCGAA 1 cut(s) 1388
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 2 cut(s) 241, 350
BsrDI GCAATG 3 cut(s) 354, 569, 663
BsrFI RCCGGY 3 cut(s) 232, 326, 512
BsrI ACTGG 3 cut(s) 261, 913, 1301
BssAI RCCGGY 3 cut(s) 232, 326, 512
BssECI CCNNGG 2 cut(s) 1131, 1173
BssMI GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
Bst4CI ACNGT 5 cut(s) 130, 277, 529, 587, 763
Bst6I CTCTTC 3 cut(s) 169, 990, 1299
BstAFI CTTAAG 1 cut(s) 95
BstBI TTCGAA 1 cut(s) 1388
BstDEI CTNAG 1 cut(s) 575
BstF5I GGATG 1 cut(s) 749
BstFNI CGCG 1 cut(s) 629
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 2 cut(s) 290, 326
BstKTI GATC 7 cut(s) 282, 701, 807, 850, 878, 1194, 1546
BstMAI GTCTC 3 cut(s) 241, 350, 1322
BstMBI GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
BstMCI CGRYCG 1 cut(s) 513
BstMWI GCNNNNNNNGC 4 cut(s) 509, 702, 896, 1175
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 828
BstUI CGCG 1 cut(s) 629
BstV1I GCAGC 4 cut(s) 282, 580, 814, 1123
BstV2I GAAGAC 4 cut(s) 93, 1317, 1463, 1538
BstZI CGGCCG 1 cut(s) 510
Bsu15I ATCGAT 2 cut(s) 384, 609
BsuRI GGCC 2 cut(s) 512, 797
BsuTUI ATCGAT 2 cut(s) 384, 609
BtgZI GCGATG 1 cut(s) 1578
BtsCI GGATG 1 cut(s) 749
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 4 cut(s) 41, 268, 331, 906
CfoI GCGC 2 cut(s) 290, 326
Cfr10I RCCGGY 3 cut(s) 232, 326, 512
Cfr13I GGNCC 2 cut(s) 439, 923
ClaI ATCGAT 2 cut(s) 384, 609
CseI GACGC 1 cut(s) 635
Csp6I GTAC 6 cut(s) 361, 893, 1019, 1080, 1237, 1465
CspAI ACCGGT 1 cut(s) 232
CviAII CATG 4 cut(s) 71, 83, 299, 308
CviQI GTAC 6 cut(s) 361, 893, 1019, 1080, 1237, 1465
DdeI CTNAG 1 cut(s) 575
DpnI GATC 7 cut(s) 281, 700, 806, 849, 877, 1193, 1545
DpnII GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
DraI TTTAAA 1 cut(s) 769
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 510
EagI CGGCCG 1 cut(s) 510
Eam1104I CTCTTC 3 cut(s) 169, 990, 1299
EarI CTCTTC 3 cut(s) 169, 990, 1299
EciI GGCGGA 2 cut(s) 555, 711
EclXI CGGCCG 1 cut(s) 510
Eco31I GGTCTC 2 cut(s) 241, 350
Eco47I GGWCC 2 cut(s) 439, 923
Eco52I CGGCCG 1 cut(s) 510
Eco88I CYCGRG 2 cut(s) 1130, 1172
EcoRI GAATTC 1 cut(s) 303
FaeI CATG 4 cut(s) 74, 86, 302, 311
FalI AAGNNNNNCTT 4 cut(s) 78, 110, 1390, 1422
FatI CATG 4 cut(s) 70, 82, 298, 307
FauNDI CATATG 1 cut(s) 1381
FbaI TGATCA 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 296, 594, 828, 1137
FokI GGATG 1 cut(s) 736
Fsp4HI GCNGC 4 cut(s) 296, 594, 828, 1137
FspAI RTGCGCAY 1 cut(s) 289
FspBI CTAG 2 cut(s) 971, 1536
FspI TGCGCA 1 cut(s) 289
GlaI GCGC 2 cut(s) 289, 325
GluI GCNGC 4 cut(s) 296, 594, 828, 1137
GsaI CCCAGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 1318
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 512, 797
HapII CCGG 8 cut(s) 233, 327, 427, 447, 509, 513, 926, 965
HgaI GACGC 1 cut(s) 635
HhaI GCGC 2 cut(s) 290, 326
Hin1II CATG 4 cut(s) 74, 86, 302, 311
Hin6I GCGC 2 cut(s) 288, 324
HinP1I GCGC 2 cut(s) 288, 324
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 2 cut(s) 13, 703
HpaII CCGG 8 cut(s) 233, 327, 427, 447, 509, 513, 926, 965
HphI GGTGA 4 cut(s) 512, 940, 1534, 1597
Hpy166II GTNNAC 7 cut(s) 24, 625, 895, 906, 931, 1363, 1588
Hpy188I TCNGA 4 cut(s) 220, 284, 321, 1344
Hpy8I GTNNAC 7 cut(s) 24, 625, 895, 906, 931, 1363, 1588
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 4 cut(s) 335, 721, 980, 1466
HpyCH4III ACNGT 5 cut(s) 130, 277, 529, 587, 763
HpyCH4IV ACGT 2 cut(s) 267, 417
HpyCH4V TGCA 4 cut(s) 298, 614, 830, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 509, 702, 896, 1175
HpyF3I CTNAG 1 cut(s) 575
HpySE526I ACGT 2 cut(s) 267, 417
Hsp92II CATG 4 cut(s) 74, 86, 302, 311
HspAI GCGC 2 cut(s) 288, 324
Kpn2I TCCGGA 1 cut(s) 964
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
LmnI GCTCC 4 cut(s) 80, 321, 449, 1403
Lsp1109I GCAGC 4 cut(s) 282, 580, 814, 1123
LweI GCATC 2 cut(s) 275, 601
MaeI CTAG 2 cut(s) 971, 1536
MaeII ACGT 2 cut(s) 267, 417
MaeIII GTNAC 3 cut(s) 263, 329, 1594
MalI GATC 7 cut(s) 281, 700, 806, 849, 877, 1193, 1545
MboI GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
MluCI AATT 9 cut(s) 303, 464, 545, 734, 756, 1140, 1367, 1414, 1507
MlyI GAGTC 4 cut(s) 155, 310, 1022, 1369
MmeI TCCRAC 3 cut(s) 102, 986, 1571
MroI TCCGGA 1 cut(s) 964
MroXI GAANNNNTTC 4 cut(s) 90, 960, 1496, 1508
MseI TTAA 6 cut(s) 96, 255, 389, 732, 768, 1199
MspA1I CMGCKG 1 cut(s) 295
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 8 cut(s) 233, 327, 427, 447, 509, 513, 926, 965
MspR9I CCNGG 1 cut(s) 509
Mva1269I GAATGC 1 cut(s) 1575
MvnI CGCG 1 cut(s) 629
MwoI GCNNNNNNNGC 4 cut(s) 509, 702, 896, 1175
NciI CCSGG 1 cut(s) 509
NdeI CATATG 1 cut(s) 1381
NdeII GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
NlaIII CATG 4 cut(s) 74, 86, 302, 311
NlaIV GGNNCC 3 cut(s) 76, 440, 840
NmeAIII GCCGAG 1 cut(s) 1159
NmuCI GTSAC 2 cut(s) 263, 329
NsbI TGCGCA 1 cut(s) 289
NspV TTCGAA 1 cut(s) 1388
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 381
PctI GAATGC 1 cut(s) 1575
PdmI GAANNNNTTC 4 cut(s) 90, 960, 1496, 1508
PfeI GAWTC 8 cut(s) 185, 207, 394, 640, 1049, 1069, 1267, 1279
PflMI CCANNNNNTGG 2 cut(s) 530, 1211
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 4 cut(s) 297, 595, 829, 1138
PleI GAGTC 4 cut(s) 155, 310, 1021, 1369
PpsI GAGTC 4 cut(s) 155, 310, 1021, 1369
PspFI CCCAGC 1 cut(s) 458
PspN4I GGNNCC 3 cut(s) 76, 440, 840
PspPI GGNCC 2 cut(s) 439, 923
PstI CTGCAG 1 cut(s) 832
PvuII CAGCTG 1 cut(s) 295
RsaI GTAC 6 cut(s) 362, 894, 1020, 1081, 1238, 1466
RsaNI GTAC 6 cut(s) 361, 893, 1019, 1080, 1237, 1465
SaqAI TTAA 6 cut(s) 96, 255, 389, 732, 768, 1199
SatI GCNGC 4 cut(s) 296, 594, 828, 1137
Sau3AI GATC 7 cut(s) 279, 698, 804, 847, 875, 1191, 1543
Sau96I GGNCC 2 cut(s) 439, 923
SchI GAGTC 4 cut(s) 155, 310, 1022, 1369
ScrFI CCNGG 1 cut(s) 509
SfaNI GCATC 2 cut(s) 275, 601
SfcI CTRYAG 1 cut(s) 828
SfuI TTCGAA 1 cut(s) 1388
SinI GGWCC 2 cut(s) 439, 923
SmlI CTYRAG 3 cut(s) 10, 95, 977
SmoI CTYRAG 3 cut(s) 10, 95, 977
Sse9I AATT 9 cut(s) 303, 464, 545, 734, 756, 1140, 1367, 1414, 1507
SsiI CCGC 4 cut(s) 540, 552, 596, 696
SspI AATATT 1 cut(s) 49
SspMI CTAG 2 cut(s) 971, 1536
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 5 cut(s) 130, 277, 529, 587, 763
TaiI ACGT 2 cut(s) 270, 420
TaqII GACCGA 2 cut(s) 390, 663
TasI AATT 9 cut(s) 303, 464, 545, 734, 756, 1140, 1367, 1414, 1507
TatI WGTACW 2 cut(s) 892, 1018
TfiI GAWTC 8 cut(s) 185, 207, 394, 640, 1049, 1069, 1267, 1279
Tru1I TTAA 6 cut(s) 96, 255, 389, 732, 768, 1199
Tru9I TTAA 6 cut(s) 96, 255, 389, 732, 768, 1199
TscAI CASTG 4 cut(s) 48, 268, 338, 913
TseFI GTSAC 2 cut(s) 263, 329
TseI GCWGC 4 cut(s) 295, 593, 827, 1136
Tsp45I GTSAC 2 cut(s) 263, 329
TspDTI ATGAA 5 cut(s) 99, 131, 296, 844, 1581
TspGWI ACGGA 1 cut(s) 1340
TspRI CASTG 4 cut(s) 48, 268, 338, 913
Van91I CCANNNNNTGG 2 cut(s) 530, 1211
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 439, 923
XapI RAATTY 2 cut(s) 303, 1367
XmnI GAANNNNTTC 4 cut(s) 90, 960, 1496, 1508
XspI CTAG 2 cut(s) 971, 1536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.