pycom05g25120

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000925
Physical Location & Seq
Forward (+)
26578642 .. 26583833
5192 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25120.1

Sequence Viewer

Length: 1281 bp
ATGGAGATCTCACACATAAAACTACTTCCCCTACTACTTCTCATCCTTCTAAGCGCAGTTTGCTCCACAACTTCGAACTCGATTTCGGAAAGCTTTGTTCAATGCTTTTCCTCCATCATACACTCTAACTCAACGAGCAACATCATTCTCACCGAAAACAGTTCGGTTTATTCCCCAGTCTTGCAATCTTCCATACAAAATCTCAGATTCTTGAACACAACACCGAAACCACAAGCTATCATTACCCCTTCTCAATACTCTCATGTTCAAGCAGCTATAGTATGCTCCAAGAAAGAGGGCATAGAAATAAGAACACGAAGCGGGGGCCATGACTATGAGGGCCTATCTTATGTATCCGAATCTCCTTTCATCATCATTGATCTTTCCAATCTTCGATCCATTGATATTGACATAAAGAAGGACAGAGCTTGGGTTGAGTCAGGTGCTACTCTTGGAGAGTTGTACTACGCAATTGCACAAAAGAGCAAACTCTATGGCTTTCCAGCAGGTATCTGTCCAACTATTGGTGTGGGAGGACACATTAGTGGAGGTGGACATGGTCCCTTGTTCAGGAAATATGGTCTAGCAGCTGACAATGTCCTTGATGCTAAGATTGTCGACGTTAATGGCAGAGTTCTTGACAGAAAATCCATGGGAGAAGAGCTCTTTTGGGCTATTCGAGGAGGCGGAGGCTCAAGCTTTGGAGTCATTGTGGCTTGGAAACTCAGGTTGGTTCCTGTTCCTCCAACTGTAACAGGTTACACGGTATCGAAGACCATAGAACAAGGTACAACCAAACTTGTATCTAAATGGCAAGCTATAGCTGACAGGATGCCTGAAGAGTATTTCCTGCGTTTGGTTATCCAAGTTGCAAATGGAGCTGGTAAAACTGGTGGAAAAACTATACAAGTTTTATTCAATTTCTTGTTTTTCGAGAATTTTAGCTTTTCCTCTTCCTCTTCCTCTTGCTTAAATTGCAGACCTCTTTTCCTCTTCCTCTTGCTTAAATTGCAGACCCTCCTCTCGCTCAAATTGCAAACCAAATTGCAGGAAAAGACTTTCCTCTTTTCCTCTCGCTCAAGTCCTGCGTCTCCTCTATCTCTTCCCTCTTGCTCATCTCTCCTCTTCGCAACCTTGATCCCCAAATCTTTAACCCAGACTTGTCTTCCCTCTCTCTTTCCCATAGACTTCTCTCTTCCTTCTCGTTCTAAACGACGGGTAGCCACACGAATCTCGAAGCCGACGGGAATCAAGTCCCAAAATCAAGGTGTAGTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

427

Amino Acids

46.4

Weight (kDa)

9.48

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 77 - 213 3.2e-29 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 497
AccB7I CCANNNNNTGG 1 cut(s) 524
AccI GTMKAC 1 cut(s) 618
AciI CCGC 2 cut(s) 321, 687
AclWI GGATC 2 cut(s) 390, 1132
AcsI RAATTY 1 cut(s) 937
AcuI CTGAAG 1 cut(s) 858
AfaI GTAC 2 cut(s) 464, 790
AfiI CCNNNNNNNGG 3 cut(s) 524, 570, 856
AgsI TTSAA 4 cut(s) 101, 214, 269, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AleI CACNNNNGTG 1 cut(s) 543
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 1 cut(s) 1095
AlwI GGATC 2 cut(s) 390, 1132
AoxI GGCC 2 cut(s) 325, 340
ApeKI GCWGC 2 cut(s) 272, 587
ApoI RAATTY 1 cut(s) 937
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 3 cut(s) 325, 340, 560
AsuHPI GGTGA 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 74
AvaII GGWCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 2 cut(s) 779, 1157
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 2 cut(s) 284, 599
BccI CCATC 1 cut(s) 122
BciVI GTATCC 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 1095
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 2 cut(s) 276, 819
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 1 cut(s) 364
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 273, 588
BlsI GCNGC 2 cut(s) 274, 589
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 3 cut(s) 325, 340, 560
BmiI GGNNCC 3 cut(s) 326, 562, 735
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 2 cut(s) 595, 822
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 2 cut(s) 779, 1157
BplI GAGNNNNNCTC 2 cut(s) 648, 680
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 2 cut(s) 679, 1063
BsaJI CCNNGG 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 3 cut(s) 524, 570, 856
Bse1I ACTGG 2 cut(s) 176, 895
BseDI CCNNGG 1 cut(s) 651
BseGI GGATG 2 cut(s) 42, 837
BseLI CCNNNNNNNGG 3 cut(s) 524, 570, 856
BseMII CTCAG 2 cut(s) 217, 739
BseNI ACTGG 2 cut(s) 176, 895
BseRI GAGGAG 4 cut(s) 696, 1010, 1083, 1112
BseXI GCAGC 2 cut(s) 284, 599
BshFI GGCC 2 cut(s) 327, 342
BsiHKAI GWGCWC 1 cut(s) 666
BslFI GGGAC 2 cut(s) 546, 1240
BslI CCNNNNNNNGG 3 cut(s) 524, 570, 856
BsmAI GTCTC 1 cut(s) 1095
BsmBI CGTCTC 1 cut(s) 1095
BsmFI GGGAC 2 cut(s) 546, 1240
BsnI GGCC 2 cut(s) 327, 342
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 4 cut(s) 6, 379, 395, 1137
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 2 cut(s) 321, 687
BspANI GGCC 2 cut(s) 327, 342
BspCNI CTCAG 2 cut(s) 216, 738
BspLI GGNNCC 3 cut(s) 326, 562, 735
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 2 cut(s) 390, 1132
BspQI GCTCTTC 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 74
BsrI ACTGG 2 cut(s) 176, 895
BssECI CCNNGG 1 cut(s) 651
BssMI GATC 4 cut(s) 6, 379, 395, 1137
BssT1I CCWWGG 1 cut(s) 651
Bst4CI ACNGT 3 cut(s) 161, 751, 766
Bst6I CTCTTC 8 cut(s) 654, 834, 958, 964, 998, 1107, 1130, 1200
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 4 cut(s) 50, 203, 609, 725
BstDSI CCRYGG 1 cut(s) 651
BstENI CCTNNNNNAGG 1 cut(s) 568
BstF5I GGATG 2 cut(s) 42, 837
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 4 cut(s) 9, 382, 398, 1140
BstMAI GTCTC 1 cut(s) 1095
BstMBI GATC 4 cut(s) 6, 379, 395, 1137
BstMWI GCNNNNNNNGC 5 cut(s) 60, 878, 975, 1009, 1033
BstSFI CTRYAG 2 cut(s) 276, 819
BstV1I GCAGC 2 cut(s) 284, 599
BstV2I GAAGAC 2 cut(s) 779, 1157
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 327, 342
BtgI CCRYGG 1 cut(s) 651
BtsCI GGATG 2 cut(s) 42, 837
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 816
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 325, 340, 560
CseI GACGC 1 cut(s) 1077
Csp6I GTAC 2 cut(s) 463, 789
CviAII CATG 4 cut(s) 263, 329, 557, 652
CviQI GTAC 2 cut(s) 463, 789
DdeI CTNAG 4 cut(s) 50, 203, 609, 725
DpnI GATC 4 cut(s) 8, 381, 397, 1139
DpnII GATC 4 cut(s) 6, 379, 395, 1137
Eam1104I CTCTTC 8 cut(s) 654, 834, 958, 964, 998, 1107, 1130, 1200
EarI CTCTTC 8 cut(s) 654, 834, 958, 964, 998, 1107, 1130, 1200
EciI GGCGGA 1 cut(s) 702
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 651
Eco24I GRGCYC 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 1 cut(s) 858
EcoICRI GAGCTC 1 cut(s) 664
EcoNI CCTNNNNNAGG 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 651
Esp3I CGTCTC 1 cut(s) 1095
FaeI CATG 4 cut(s) 266, 332, 560, 655
FaqI GGGAC 2 cut(s) 546, 1240
FatI CATG 4 cut(s) 262, 328, 556, 651
FauI CCCGC 1 cut(s) 314
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 2 cut(s) 273, 588
FokI GGATG 2 cut(s) 29, 844
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 2 cut(s) 273, 588
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 273, 588
HaeIII GGCC 2 cut(s) 327, 342
HgaI GACGC 1 cut(s) 1077
HhaI GCGC 1 cut(s) 56
Hin1II CATG 4 cut(s) 266, 332, 560, 655
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 2 cut(s) 91, 697
HinfI GANTC 6 cut(s) 207, 359, 437, 705, 1230, 1248
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 554, 619
Hpy188I TCNGA 3 cut(s) 88, 206, 358
Hpy188III TCNNGA 5 cut(s) 211, 571, 638, 934, 1234
Hpy8I GTNNAC 2 cut(s) 554, 619
Hpy99I CGWCG 3 cut(s) 623, 1218, 1246
HpyAV CCTTC 4 cut(s) 56, 258, 412, 1209
HpyCH4III ACNGT 3 cut(s) 161, 751, 766
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 7 cut(s) 184, 476, 872, 978, 1012, 1036, 1048
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 878, 975, 1009, 1033
HpyF3I CTNAG 4 cut(s) 50, 203, 609, 725
HpySE526I ACGT 1 cut(s) 621
Hsp92II CATG 4 cut(s) 266, 332, 560, 655
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 4 cut(s) 6, 379, 395, 1137
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 3 cut(s) 68, 290, 878
Lsp1109I GCAGC 2 cut(s) 284, 599
LweI GCATC 2 cut(s) 595, 822
MaeI CTAG 1 cut(s) 584
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 2 cut(s) 751, 758
MalI GATC 4 cut(s) 8, 381, 397, 1139
MboI GATC 4 cut(s) 6, 379, 395, 1137
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 7 cut(s) 471, 919, 937, 973, 1007, 1031, 1043
MlyI GAGTC 2 cut(s) 446, 714
MmeI TCCRAC 2 cut(s) 542, 770
MseI TTAA 4 cut(s) 624, 971, 1005, 1151
MslI CAYNNNNRTG 2 cut(s) 333, 543
MspA1I CMGCKG 1 cut(s) 590
MunI CAATTG 1 cut(s) 471
MwoI GCNNNNNNNGC 5 cut(s) 60, 878, 975, 1009, 1033
NcoI CCATGG 1 cut(s) 651
NdeII GATC 4 cut(s) 6, 379, 395, 1137
NlaIII CATG 4 cut(s) 266, 332, 560, 655
NlaIV GGNNCC 3 cut(s) 326, 562, 735
NspV TTCGAA 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 1210
PfeI GAWTC 4 cut(s) 207, 359, 1230, 1248
PflFI GACNNNGTC 2 cut(s) 558, 596
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 2 cut(s) 274, 589
PleI GAGTC 2 cut(s) 445, 713
PpsI GAGTC 2 cut(s) 445, 713
Psp124BI GAGCTC 1 cut(s) 666
PspN4I GGNNCC 3 cut(s) 326, 562, 735
PspPI GGNCC 3 cut(s) 325, 340, 560
PsuI RGATCY 1 cut(s) 6
PsyI GACNNNGTC 2 cut(s) 558, 596
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 2 cut(s) 464, 790
RsaNI GTAC 2 cut(s) 463, 789
RseI CAYNNNNRTG 2 cut(s) 333, 543
SacI GAGCTC 1 cut(s) 666
SalI GTCGAC 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 4 cut(s) 624, 971, 1005, 1151
SatI GCNGC 2 cut(s) 273, 588
Sau3AI GATC 4 cut(s) 6, 379, 395, 1137
Sau96I GGNCC 3 cut(s) 325, 340, 560
SchI GAGTC 2 cut(s) 446, 714
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 2 cut(s) 595, 822
SfcI CTRYAG 2 cut(s) 276, 819
SfuI TTCGAA 1 cut(s) 74
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 2 cut(s) 333, 543
SmlI CTYRAG 2 cut(s) 694, 1078
SmoI CTYRAG 2 cut(s) 694, 1078
Sse9I AATT 7 cut(s) 471, 919, 937, 973, 1007, 1031, 1043
SsiI CCGC 2 cut(s) 321, 687
SspMI CTAG 1 cut(s) 584
SstI GAGCTC 1 cut(s) 666
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 3 cut(s) 161, 751, 766
TaiI ACGT 1 cut(s) 624
TaqI TCGA 8 cut(s) 74, 80, 394, 618, 679, 770, 933, 1235
TasI AATT 7 cut(s) 471, 919, 937, 973, 1007, 1031, 1043
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 4 cut(s) 207, 359, 1230, 1248
Tru1I TTAA 4 cut(s) 624, 971, 1005, 1151
Tru9I TTAA 4 cut(s) 624, 971, 1005, 1151
TseI GCWGC 2 cut(s) 272, 587
TspDTI ATGAA 1 cut(s) 358
Tth111I GACNNNGTC 2 cut(s) 558, 596
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 560
XagI CCTNNNNNAGG 1 cut(s) 568
XapI RAATTY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 618
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.