Rw5G011350

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000925
Physical Location & Seq
Reverse (-)
12907523 .. 12909097
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011350.1

Sequence Viewer

Length: 1575 bp
ATGGAGACCTTTCATGTAAAACTATTTTCCCTACTTTTCATCCTACTCAACTCAGTTTGGTCAACAACTTCAACATCAAATTCTGAAAGCTTTGTTCAATGCTTTTCCTCCCACCTACAGAACTCTAACTCCAGTAATGAAATCATTCTCCCCATAACCAGTTCTGCTTATCCCTCAGTCCTAGACTTTTCCATACAGAACCTCAGATTCCTGAACAATTCCACACCTAAACCAGAGGCCATCATTACTCCTTTTGATGAATCTCAGGTTCAAGCAGCTGTAATTTGCTCCAAGAAAAGTGGCATACAAATAAGAATCCGAAGCGGGGGTCATGATTACGAGGGCCTATCTTATGTGTCTAGAGCTCCTTTCATCATCATTGACCTTTTCAATCTCCGTTCCATCGATGTTGATATAAAGAATGAGAGTGCTTGGGTCAAATCAGGCGCTACTCTTGGAGAGTTGTATTACAACATTGCACAGAAGAGTAAAGTCTATGGCTTTCCAGCAGGGACTTGTCCCACCGTCGGTGTTGGGGGACACATCAGTGGGGGTGGAATTGGAACCATATTCCGAAAATATGGTCTAGCATCCGACAATGTCATTGATGCTAGAATTGTAGATGTTAACGGCAGAATTCTAGACAGGAAGTCCATGGGAGAAGAGCTCTTTTGGGCTATCAGAGGAGGAGGAGGATCAAGCTTTGGAGTCATTCTAGCATGGAAACTTAGACTAGTTCCTGTTCCCCCAACTGTAACAGTTTGCGACGTTACAAAGACAAAAGAACAAGGTGCAACCAAACTTCTTTTGAAATGGCAAAACATTGCAGACAAGCTTCCTGAAGACCTTTTCCTACATTCAGTTGTAGGAAGTAGTGACAACACTATTACAGTTGAATTCGGCTCCCTGTTTCTTGGGCAGGTCGACAAAGCTGTCCAGGTGATACAGGAAAACTTCCCAGAGTTGAGTTTAGAGAGCAGCCATTGCAGTGAGATGAGTTGGATTCAGTCTGTTTTGGACTCTGCTGGTTACTCGATACACGAATCCTTGGAGATTTTGCTGAAGCGCCGGCAATACAGCTACTTCTTCAAAGGAAAATCAGACTTTGTGACTGAACCCATCTCAGAAGCTGGCTTGGAAGGCTTATGGCACATATTGCTTGAAGCAAACACATCCTTTATGATATTGACACCATATGGAGGAAAAATGAGTGAGATTTCCGATTCAGAAACTGCTTTCCCACATCGAAAAGGAAACAAATACGAAATCCAGTATATGGTTACTTGGGGTGATGGCAAGGACACGAAGAAGTATGTTGGATTTATGAGAAGGCTGTATGCATACATGGCCCCGTATGTTTCAAAGTCTCCTAGAGCTGCCTATTTGAATTATAGGGATCTTGATTTGGGGAGGAACAGTCATGGCAATACAAGCTATGCACAAGCAAGCATTTGGGGTTTGAAGTACTTCAAGAACAACTTTAGGAGACTTGTTCATGTTAAGACCTTGGTTGATCCTGGCAACTTCTTTAGGAATGAGCAAAGCATCCCCGTGTTTTCGTCAGGAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

524

Amino Acids

58.39

Weight (kDa)

8.54

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 78 - 214 3.3e-26 FAD binding domain
BBE PF08031 460 - 517 1.6e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 1276
AccI GTMKAC 1 cut(s) 924
AciI CCGC 1 cut(s) 324
AclWI GGATC 3 cut(s) 703, 1404, 1508
AcsI RAATTY 3 cut(s) 79, 636, 896
AcuI CTGAAG 2 cut(s) 861, 1082
AfaI GTAC 1 cut(s) 1466
AfiI CCNNNNNNNGG 4 cut(s) 325, 527, 1199, 1276
AhdI GACNNNNNGTC 1 cut(s) 649
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 2 cut(s) 936, 1516
AleI CACNNNNGTG 1 cut(s) 546
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
Alw21I GWGCWC 2 cut(s) 367, 669
Alw26I GTCTC 2 cut(s) 1371, 1480
AlwI GGATC 3 cut(s) 703, 1404, 1508
AlwNI CAGNNNCTG 2 cut(s) 1130, 1232
AoxI GGCC 3 cut(s) 237, 343, 1347
ApeKI GCWGC 3 cut(s) 275, 978, 1376
ApoI RAATTY 3 cut(s) 79, 636, 896
ArsI GACNNNNNNTTYG 4 cut(s) 313, 345, 568, 600
Asp700I GAANNNNTTC 2 cut(s) 144, 1466
AspLEI GCGC 2 cut(s) 449, 1068
AspS9I GGNCC 2 cut(s) 343, 1348
AsuHPI GGTGA 2 cut(s) 952, 1301
BanII GRGCYC 2 cut(s) 367, 669
BbsI GAAGAC 1 cut(s) 849
Bbv12I GWGCWC 2 cut(s) 367, 669
BbvI GCAGC 3 cut(s) 287, 990, 1363
BccI CCATC 4 cut(s) 248, 410, 1127, 1286
BceAI ACGGC 1 cut(s) 646
BciT130I CCWGG 2 cut(s) 938, 1518
BcoDI GTCTC 2 cut(s) 1371, 1480
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 8 cut(s) 182, 360, 587, 612, 641, 716, 734, 1371
BfmI CTRYAG 1 cut(s) 116
BfoI RGCGCY 2 cut(s) 450, 1069
BfuAI ACCTGC 1 cut(s) 910
BisI GCNGC 3 cut(s) 276, 979, 1377
BlsI GCNGC 3 cut(s) 277, 980, 1378
BmcAI AGTACT 1 cut(s) 1466
Bme1390I CCNGG 2 cut(s) 938, 1518
BmeRI GACNNNNNGTC 1 cut(s) 649
BmgT120I GGNCC 2 cut(s) 343, 1348
BmiI GGNNCC 3 cut(s) 565, 904, 1350
BmrFI CCNGG 2 cut(s) 938, 1518
BmsI GCATC 3 cut(s) 598, 599, 1554
BpiI GAAGAC 1 cut(s) 849
BplI GAGNNNNNCTC 2 cut(s) 651, 683
BpmI CTGGAG 1 cut(s) 115
Bsa29I ATCGAT 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 654, 1047, 1506
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 1402, 1432
Bsc4I CCNNNNNNNGG 4 cut(s) 325, 527, 1199, 1276
Bse118I RCCGGY 1 cut(s) 1068
Bse1I ACTGG 3 cut(s) 132, 159, 1270
Bse3DI GCAATG 3 cut(s) 474, 822, 982
BseBI CCWGG 2 cut(s) 938, 1518
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 3 cut(s) 654, 1047, 1506
BseGI GGATG 4 cut(s) 39, 590, 1172, 1545
BseLI CCNNNNNNNGG 4 cut(s) 325, 527, 1199, 1276
BseMI GCAATG 3 cut(s) 474, 822, 982
BseMII CTCAG 5 cut(s) 66, 189, 217, 278, 1137
BseNI ACTGG 3 cut(s) 132, 159, 1270
BseRI GAGGAG 3 cut(s) 699, 702, 705
BseXI GCAGC 3 cut(s) 287, 990, 1363
BshFI GGCC 3 cut(s) 239, 345, 1349
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 2 cut(s) 367, 669
BsiSI CCGG 1 cut(s) 1069
BslFI GGGAC 3 cut(s) 504, 526, 552
BslI CCNNNNNNNGG 4 cut(s) 325, 527, 1199, 1276
BsmAI GTCTC 2 cut(s) 1371, 1480
BsmFI GGGAC 3 cut(s) 504, 526, 552
BsnI GGCC 3 cut(s) 239, 345, 1349
Bsp1286I GDGCHC 2 cut(s) 367, 669
Bsp143I GATC 3 cut(s) 695, 1396, 1513
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 1 cut(s) 324
BspANI GGCC 3 cut(s) 239, 345, 1349
BspCNI CTCAG 5 cut(s) 65, 188, 216, 277, 1136
BspDI ATCGAT 1 cut(s) 405
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 565, 904, 1350
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 3 cut(s) 703, 1404, 1508
BspQI GCTCTTC 1 cut(s) 657
BsrDI GCAATG 3 cut(s) 474, 822, 982
BsrFI RCCGGY 1 cut(s) 1068
BsrI ACTGG 3 cut(s) 132, 159, 1270
BssAI RCCGGY 1 cut(s) 1068
BssECI CCNNGG 3 cut(s) 654, 1047, 1506
BssMI GATC 3 cut(s) 695, 1396, 1513
BssT1I CCWWGG 3 cut(s) 654, 1047, 1506
Bst2UI CCWGG 2 cut(s) 938, 1518
Bst4CI ACNGT 5 cut(s) 526, 754, 760, 892, 1418
Bst6I CTCTTC 2 cut(s) 479, 657
BstAPI GCANNNNNTGC 2 cut(s) 984, 1156
BstC8I GCNNGC 3 cut(s) 1070, 1132, 1447
BstDEI CTNAG 6 cut(s) 52, 175, 203, 264, 728, 1123
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 4 cut(s) 39, 590, 1172, 1545
BstH2I RGCGCY 2 cut(s) 450, 1069
BstHHI GCGC 2 cut(s) 449, 1068
BstKTI GATC 3 cut(s) 698, 1399, 1516
BstMAI GTCTC 2 cut(s) 1371, 1480
BstMBI GATC 3 cut(s) 695, 1396, 1513
BstMWI GCNNNNNNNGC 5 cut(s) 984, 1140, 1156, 1346, 1431
BstNI CCWGG 2 cut(s) 938, 1518
BstSCI CCNGG 2 cut(s) 936, 1516
BstSFI CTRYAG 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 287, 990, 1363
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 1 cut(s) 1396
BstYI RGATCY 1 cut(s) 1396
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 3 cut(s) 239, 345, 1349
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 39, 590, 1172, 1545
BtsI GCAGTG 1 cut(s) 994
BtsIMutI CAGTG 2 cut(s) 553, 994
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 3 cut(s) 1070, 1132, 1447
CaiI CAGNNNCTG 2 cut(s) 1130, 1232
CciI TCATGA 1 cut(s) 331
CfoI GCGC 2 cut(s) 449, 1068
Cfr10I RCCGGY 1 cut(s) 1068
Cfr13I GGNCC 2 cut(s) 343, 1348
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 1 cut(s) 1465
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviAII CATG 7 cut(s) 14, 332, 655, 720, 1345, 1421, 1496
CviQI GTAC 1 cut(s) 1465
DdeI CTNAG 6 cut(s) 52, 175, 203, 264, 728, 1123
DpnI GATC 3 cut(s) 697, 1398, 1515
DpnII GATC 3 cut(s) 695, 1396, 1513
DrdI GACNNNNNNGTC 1 cut(s) 932
DriI GACNNNNNGTC 1 cut(s) 649
DseDI GACNNNNNNGTC 1 cut(s) 932
Eam1104I CTCTTC 2 cut(s) 479, 657
Eam1105I GACNNNNNGTC 1 cut(s) 649
EarI CTCTTC 2 cut(s) 479, 657
Ecl136II GAGCTC 2 cut(s) 365, 667
Eco130I CCWWGG 3 cut(s) 654, 1047, 1506
Eco24I GRGCYC 2 cut(s) 367, 669
Eco53kI GAGCTC 2 cut(s) 365, 667
Eco57I CTGAAG 2 cut(s) 861, 1082
EcoICRI GAGCTC 2 cut(s) 365, 667
EcoO109I RGGNCCY 1 cut(s) 343
EcoRI GAATTC 2 cut(s) 636, 896
EcoRII CCWGG 2 cut(s) 936, 1516
EcoT14I CCWWGG 3 cut(s) 654, 1047, 1506
EcoT22I ATGCAT 1 cut(s) 1342
EcoT38I GRGCYC 2 cut(s) 367, 669
ErhI CCWWGG 3 cut(s) 654, 1047, 1506
FaeI CATG 7 cut(s) 17, 335, 658, 723, 1348, 1424, 1499
FalI AAGNNNNNCTT 2 cut(s) 1463, 1495
FaqI GGGAC 3 cut(s) 504, 526, 552
FatI CATG 7 cut(s) 13, 331, 654, 719, 1344, 1420, 1495
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 1 cut(s) 1195
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 3 cut(s) 276, 979, 1377
FokI GGATG 4 cut(s) 26, 577, 1159, 1532
FriOI GRGCYC 2 cut(s) 367, 669
Fsp4HI GCNGC 3 cut(s) 276, 979, 1377
FspBI CTAG 8 cut(s) 182, 360, 587, 612, 641, 716, 734, 1371
GlaI GCGC 2 cut(s) 448, 1067
GluI GCNGC 3 cut(s) 276, 979, 1377
GsuI CTGGAG 1 cut(s) 115
HaeII RGCGCY 2 cut(s) 450, 1069
HaeIII GGCC 3 cut(s) 239, 345, 1349
HapII CCGG 1 cut(s) 1069
HhaI GCGC 2 cut(s) 449, 1068
Hin1II CATG 7 cut(s) 17, 335, 658, 723, 1348, 1424, 1499
Hin6I GCGC 2 cut(s) 447, 1066
HinP1I GCGC 2 cut(s) 447, 1066
HincII GTYRAC 3 cut(s) 63, 628, 925
HindII GTYRAC 3 cut(s) 63, 628, 925
HindIII AAGCTT 3 cut(s) 88, 700, 833
HinfI GANTC 8 cut(s) 207, 260, 315, 708, 1003, 1019, 1043, 1223
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 1 cut(s) 1069
HphI GGTGA 2 cut(s) 952, 1301
Hpy166II GTNNAC 3 cut(s) 63, 628, 925
Hpy188III TCNNGA 8 cut(s) 211, 332, 360, 641, 839, 1400, 1471, 1563
Hpy8I GTNNAC 3 cut(s) 63, 628, 925
Hpy99I CGWCG 2 cut(s) 530, 770
HpyAV CCTTC 2 cut(s) 1133, 1323
HpyCH4III ACNGT 5 cut(s) 526, 754, 760, 892, 1418
HpyCH4IV ACGT 1 cut(s) 768
HpyCH4V TGCA 6 cut(s) 479, 794, 827, 987, 1340, 1439
HpyF10VI GCNNNNNNNGC 5 cut(s) 984, 1140, 1156, 1346, 1431
HpyF3I CTNAG 6 cut(s) 52, 175, 203, 264, 728, 1123
HpySE526I ACGT 1 cut(s) 768
Hsp92II CATG 7 cut(s) 17, 335, 658, 723, 1348, 1424, 1499
HspAI GCGC 2 cut(s) 447, 1066
KroI GCCGGC 1 cut(s) 1068
KroNI GCCGGC 1 cut(s) 1070
KspAI GTTAAC 1 cut(s) 628
Kzo9I GATC 3 cut(s) 695, 1396, 1513
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 3 cut(s) 293, 370, 908
Lsp1109I GCAGC 3 cut(s) 287, 990, 1363
LweI GCATC 3 cut(s) 598, 599, 1554
MaeI CTAG 8 cut(s) 182, 360, 587, 612, 641, 716, 734, 1371
MaeII ACGT 1 cut(s) 768
MaeIII GTNAC 6 cut(s) 754, 769, 875, 1028, 1108, 1279
MalI GATC 3 cut(s) 697, 1398, 1515
MboI GATC 3 cut(s) 695, 1396, 1513
MboII GAAGA 5 cut(s) 496, 674, 854, 1078, 1318
MflI RGATCY 1 cut(s) 1396
MhlI GDGCHC 2 cut(s) 367, 669
MluCI AATT 8 cut(s) 79, 217, 282, 558, 615, 636, 896, 1387
MlyI GAGTC 2 cut(s) 717, 1013
MmeI TCCRAC 3 cut(s) 618, 980, 1297
Mph1103I ATGCAT 1 cut(s) 1342
MroNI GCCGGC 1 cut(s) 1068
MroXI GAANNNNTTC 2 cut(s) 144, 1466
MseI TTAA 2 cut(s) 627, 1500
MslI CAYNNNNRTG 3 cut(s) 546, 987, 1550
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 1 cut(s) 1069
MspR9I CCNGG 2 cut(s) 938, 1518
MvaI CCWGG 2 cut(s) 938, 1518
MwoI GCNNNNNNNGC 5 cut(s) 984, 1140, 1156, 1346, 1431
NaeI GCCGGC 1 cut(s) 1070
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1195
NdeII GATC 3 cut(s) 695, 1396, 1513
NgoMIV GCCGGC 1 cut(s) 1068
NlaIII CATG 7 cut(s) 17, 335, 658, 723, 1348, 1424, 1499
NlaIV GGNNCC 3 cut(s) 565, 904, 1350
NmuCI GTSAC 2 cut(s) 875, 1108
NsiI ATGCAT 1 cut(s) 1342
OliI CACNNNNGTG 1 cut(s) 546
PagI TCATGA 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 657
PdiI GCCGGC 1 cut(s) 1070
PdmI GAANNNNTTC 2 cut(s) 144, 1466
PfeI GAWTC 6 cut(s) 207, 260, 315, 1003, 1043, 1223
PflFI GACNNNGTC 1 cut(s) 599
PflMI CCANNNNNTGG 1 cut(s) 1276
PkrI GCNGC 3 cut(s) 277, 980, 1378
PleI GAGTC 2 cut(s) 716, 1013
PpsI GAGTC 2 cut(s) 716, 1013
Psp124BI GAGCTC 2 cut(s) 367, 669
Psp6I CCWGG 2 cut(s) 936, 1516
PspGI CCWGG 2 cut(s) 936, 1516
PspN4I GGNNCC 3 cut(s) 565, 904, 1350
PspPI GGNCC 2 cut(s) 343, 1348
PstNI CAGNNNCTG 2 cut(s) 1130, 1232
PsuI RGATCY 1 cut(s) 1396
PsyI GACNNNGTC 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 1 cut(s) 1466
RsaNI GTAC 1 cut(s) 1465
RseI CAYNNNNRTG 3 cut(s) 546, 987, 1550
SacI GAGCTC 2 cut(s) 367, 669
SalI GTCGAC 1 cut(s) 923
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 627, 1500
SatI GCNGC 3 cut(s) 276, 979, 1377
Sau3AI GATC 3 cut(s) 695, 1396, 1513
Sau96I GGNCC 2 cut(s) 343, 1348
ScaI AGTACT 1 cut(s) 1466
SchI GAGTC 2 cut(s) 717, 1013
ScrFI CCNGG 2 cut(s) 938, 1518
SduI GDGCHC 2 cut(s) 367, 669
SfaNI GCATC 3 cut(s) 598, 599, 1554
SfcI CTRYAG 1 cut(s) 116
SmiMI CAYNNNNRTG 3 cut(s) 546, 987, 1550
SpeI ACTAGT 1 cut(s) 733
Sse9I AATT 8 cut(s) 79, 217, 282, 558, 615, 636, 896, 1387
SsiI CCGC 1 cut(s) 324
SspMI CTAG 8 cut(s) 182, 360, 587, 612, 641, 716, 734, 1371
SstI GAGCTC 2 cut(s) 367, 669
StyD4I CCNGG 2 cut(s) 936, 1516
StyI CCWWGG 3 cut(s) 654, 1047, 1506
TaaI ACNGT 5 cut(s) 526, 754, 760, 892, 1418
TaiI ACGT 1 cut(s) 771
TaqI TCGA 4 cut(s) 405, 924, 1034, 1246
TasI AATT 8 cut(s) 79, 217, 282, 558, 615, 636, 896, 1387
TatI WGTACW 1 cut(s) 1464
TfiI GAWTC 6 cut(s) 207, 260, 315, 1003, 1043, 1223
Tru1I TTAA 2 cut(s) 627, 1500
Tru9I TTAA 2 cut(s) 627, 1500
TscAI CASTG 2 cut(s) 553, 994
TseFI GTSAC 2 cut(s) 875, 1108
TseI GCWGC 3 cut(s) 275, 978, 1376
Tsp45I GTSAC 2 cut(s) 875, 1108
TspDTI ATGAA 5 cut(s) 28, 153, 273, 361, 1484
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 2 cut(s) 553, 994
Tth111I GACNNNGTC 1 cut(s) 599
Van91I CCANNNNNTGG 1 cut(s) 1276
XapI RAATTY 3 cut(s) 79, 636, 896
XbaI TCTAGA 2 cut(s) 359, 640
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 2 cut(s) 144, 1466
XspI CTAG 8 cut(s) 182, 360, 587, 612, 641, 716, 734, 1371
ZrmI AGTACT 1 cut(s) 1466
Zsp2I ATGCAT 1 cut(s) 1342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.