MD05G1268100.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000925
Physical Location & Seq
Forward (+)
40342330 .. 40343926
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1268100.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGAGATCTCGAATGTAAAAGTACTTCAACTACTACTCTTCATCCTTCTCAACTTAGTTTGTTCCACAACTTCAAACTCAAATTCCCAAAGATTTCTTCAATGCTTTTCCTCCAACATAAATTCTAACCCATCAAGCAAAATCATTCTCACCAAAAACTCTCCTGCTTACTTCTCAGTCTTGCAATCTTCTATACAGAATCTCCGATTCTTGAACAATTCAACACGAAAACCACTAGCTATCGTTACTCCTTTTCAGTACTCTCATGTTCAAGCAGCGGTAAAATGCTCCAAGAAAGAAGGCATACAAATAAGAAGCCGAAGCGGGGGCCATGACTACGAGGGTCTGTCTTATGTATCCAAAACTTCTTTCATCATCATCGATCTTTTCAATCTTCGTTCCATTGATATTGAAATAGAGAATGGGAGTGCTTGGGTCGAATCAGGTGCTACTCTGGGAGAGTTGTACTACGCAATTGCACAAAAGAGTAAAGTCTATGGATTTCCAGCAGGGACATGTCCAACCATTGGTGTAGGGGGACATATTAGTGGAGGTGGACATGGTACCATGTTCAGAAAATATGGCCTTGCAGCTGACAATGTCCTTGATGCTAAGATTGTCGATGTCAATGGAAGAGTTCTTGACAGAAAATCCATGGGAGATGAGCTCTTTTGGGCTATTCGAGGAGGTGGAGGCTCAAGCTTTGGAGTCATTTTGGCATGGAAGCTTAGGTTGGTTCCTGTTCCTGCAACCGTAACAGTTTACTCAGAAGCAAGGACCATTGAACAAGACAGGATTCCTGAAGAGTACGTCCTGCGTTTGGTGTTCCAAGTCGCAAACGGAGCTGGTACAAATGACGGAAAAACTATTCAAGTTATATTCAATTTCGTGTTTCTCGAGTCTGTTGAGAAACTTCTCCCTTGGATGCAAGAAAATTTCCCAGAGTTGAGTTTAGACAAGACCAAATTCAATGAAATGAGTTGGATTGAGTCTGTCCTATATATTTCTGGCATCCCAAGAAACGAATCAGAAACTTTGCTTCAGCGGACGCAACAATCGAAGGGATTCTTCAAAGCGAAATCAGACTATGTTACTGAACCAATTTCAGAAGCTGGCTTGGAAGGTTTATGGGAAAGACTGCTTCAGCTAGAGATATTCCAGTTGATACTGACACCTTTCGGAGGAATAATGAGCGAGGTTTCTGATTCAGAAATTCCTTTCCCACATAGAAAAGGAACTTTATTTGAAATCCAGTATTTGGTCAGTTGGGGTGATGATAAAGAAACTGAGAAACATATTGGCTCGATGAAAATGCTGTACGAGTACATGACACCATATGTTTCGAAGTCCCCGAGAGCTGCCTATTTGAACTATAGAGATCTTGACTTGGGGACGAACAAAAACGGTAACACTAGCTATGCACAAGCAAGCATTTGGGGTTTGAGGTATTTTAAGAATAACTTTAGGAGACTAGTACATGTGAAGACTTTAGTTGATCCTGAGAACTTCTTCAGGAATGAGCAAAGCATCCCGGTGTTACAATCTGGGGAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

519

Amino Acids

58.17

Weight (kDa)

8.12

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 78 - 214 1.6e-28 FAD binding domain
BBE PF08031 454 - 511 6.2e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 563
AccB1I GGYRCC 1 cut(s) 563
AccB7I CCANNNNNTGG 2 cut(s) 527, 1258
AciI CCGC 3 cut(s) 278, 324, 1045
AclWI GGATC 1 cut(s) 1490
AcsI RAATTY 5 cut(s) 82, 121, 934, 965, 1212
AcuI CTGAAG 4 cut(s) 822, 1025, 1127, 1495
AfaI GTAC 9 cut(s) 24, 260, 467, 565, 809, 850, 1319, 1325, 1476
AfiI CCNNNNNNNGG 5 cut(s) 325, 527, 820, 1181, 1258
AflIII ACRYGT 2 cut(s) 515, 1477
AhlI ACTAGT 1 cut(s) 1471
AjuI GAANNNNNNNTTGG 6 cut(s) 81, 113, 353, 385, 716, 748
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 1462
AlwI GGATC 1 cut(s) 1490
AlwNI CAGNNNCTG 1 cut(s) 1112
Ama87I CYCGRG 2 cut(s) 896, 1351
AoxI GGCC 2 cut(s) 328, 583
ApeKI GCWGC 3 cut(s) 275, 590, 1358
ApoI RAATTY 5 cut(s) 82, 121, 934, 965, 1212
Asp700I GAANNNNTTC 2 cut(s) 1064, 1508
Asp718I GGTACC 1 cut(s) 563
AspS9I GGNCC 2 cut(s) 328, 777
AsuC2I CCSGG 1 cut(s) 1532
AsuHPI GGTGA 2 cut(s) 142, 1283
AsuII TTCGAA 1 cut(s) 1343
AvaI CYCGRG 2 cut(s) 896, 1351
AvaII GGWCC 1 cut(s) 777
BanI GGYRCC 1 cut(s) 563
BanII GRGCYC 1 cut(s) 669
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 1 cut(s) 1490
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 3 cut(s) 287, 602, 1345
BccI CCATC 1 cut(s) 139
BcgI CGANNNNNNTGC 4 cut(s) 428, 462, 1294, 1328
BciVI GTATCC 1 cut(s) 367
BcnI CCSGG 1 cut(s) 1532
BcoDI GTCTC 1 cut(s) 1462
BcuI ACTAGT 1 cut(s) 1471
BfaI CTAG 4 cut(s) 236, 1148, 1413, 1472
BfmI CTRYAG 1 cut(s) 1372
BfuI GTATCC 1 cut(s) 367
BglII AGATCT 2 cut(s) 6, 1378
BisI GCNGC 3 cut(s) 276, 591, 1359
BlsI GCNGC 3 cut(s) 277, 592, 1360
BmcAI AGTACT 2 cut(s) 24, 260
Bme1390I CCNGG 1 cut(s) 1532
Bme18I GGWCC 1 cut(s) 777
BmeT110I CYCGRG 2 cut(s) 896, 1351
BmgT120I GGNCC 2 cut(s) 328, 777
BmiI GGNNCC 3 cut(s) 329, 565, 738
BmrFI CCNGG 1 cut(s) 1532
BmsI GCATC 4 cut(s) 598, 915, 1020, 1536
BpiI GAAGAC 1 cut(s) 1490
BplI GAGNNNNNCTC 2 cut(s) 651, 683
Bpu10I CCTNAGC 1 cut(s) 728
Bpu14I TTCGAA 1 cut(s) 1343
BpuEI CTTGAG 1 cut(s) 682
BpuMI CCSGG 1 cut(s) 1532
Bsa29I ATCGAT 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 654, 920
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 5 cut(s) 325, 527, 820, 1181, 1258
Bse1I ACTGG 2 cut(s) 1159, 1252
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 2 cut(s) 654, 920
BseGI GGATG 4 cut(s) 42, 930, 1011, 1527
BseLI CCNNNNNNNGG 5 cut(s) 325, 527, 820, 1181, 1258
BseMII CTCAG 4 cut(s) 189, 780, 1278, 1491
BseNI ACTGG 2 cut(s) 1159, 1252
BseRI GAGGAG 1 cut(s) 699
BseXI GCAGC 3 cut(s) 287, 602, 1345
BshFI GGCC 2 cut(s) 330, 585
BshNI GGYRCC 1 cut(s) 563
BshVI ATCGAT 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 2 cut(s) 896, 1351
BsiSI CCGG 1 cut(s) 1532
BslFI GGGAC 4 cut(s) 526, 552, 1333, 1405
BslI CCNNNNNNNGG 5 cut(s) 325, 527, 820, 1181, 1258
BsmAI GTCTC 1 cut(s) 1462
BsmFI GGGAC 4 cut(s) 526, 552, 1333, 1405
BsnI GGCC 2 cut(s) 330, 585
BsoBI CYCGRG 2 cut(s) 896, 1351
Bsp119I TTCGAA 1 cut(s) 1343
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 4 cut(s) 6, 382, 1378, 1495
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 3 cut(s) 278, 324, 1045
BspANI GGCC 2 cut(s) 330, 585
BspCNI CTCAG 4 cut(s) 188, 779, 1279, 1492
BspDI ATCGAT 1 cut(s) 381
BspLI GGNNCC 3 cut(s) 329, 565, 738
BspPI GGATC 1 cut(s) 1490
BspT104I TTCGAA 1 cut(s) 1343
BspT107I GGYRCC 1 cut(s) 563
BsrI ACTGG 2 cut(s) 1159, 1252
BssECI CCNNGG 2 cut(s) 654, 920
BssMI GATC 4 cut(s) 6, 382, 1378, 1495
BssT1I CCWWGG 2 cut(s) 654, 920
Bst4CI ACNGT 3 cut(s) 754, 760, 1406
Bst6I CTCTTC 3 cut(s) 44, 628, 798
BstBI TTCGAA 1 cut(s) 1343
BstC8I GCNNGC 2 cut(s) 1114, 1429
BstDEI CTNAG 7 cut(s) 55, 175, 612, 728, 766, 1287, 1500
BstDSI CCRYGG 1 cut(s) 654
BstENI CCTNNNNNAGG 1 cut(s) 1179
BstF5I GGATG 4 cut(s) 42, 930, 1011, 1527
BstKTI GATC 4 cut(s) 9, 385, 1381, 1498
BstMAI GTCTC 1 cut(s) 1462
BstMBI GATC 4 cut(s) 6, 382, 1378, 1495
BstMWI GCNNNNNNNGC 1 cut(s) 842
BstNSI RCATGY 2 cut(s) 519, 1481
BstSCI CCNGG 1 cut(s) 1530
BstSFI CTRYAG 1 cut(s) 1372
BstV1I GCAGC 3 cut(s) 287, 602, 1345
BstV2I GAAGAC 1 cut(s) 1490
BstX2I RGATCY 2 cut(s) 6, 1378
BstYI RGATCY 2 cut(s) 6, 1378
Bsu15I ATCGAT 1 cut(s) 381
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 330, 585
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 42, 930, 1011, 1527
Cac8I GCNNGC 2 cut(s) 1114, 1429
CaiI CAGNNNCTG 1 cut(s) 1112
Cfr13I GGNCC 2 cut(s) 328, 777
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 1057
Csp6I GTAC 9 cut(s) 23, 259, 466, 564, 808, 849, 1318, 1324, 1475
CviAII CATG 9 cut(s) 266, 332, 516, 560, 568, 655, 720, 1327, 1478
CviQI GTAC 9 cut(s) 23, 259, 466, 564, 808, 849, 1318, 1324, 1475
DdeI CTNAG 7 cut(s) 55, 175, 612, 728, 766, 1287, 1500
DpnI GATC 4 cut(s) 8, 384, 1380, 1497
DpnII GATC 4 cut(s) 6, 382, 1378, 1495
Eam1104I CTCTTC 3 cut(s) 44, 628, 798
EarI CTCTTC 3 cut(s) 44, 628, 798
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 2 cut(s) 654, 920
Eco24I GRGCYC 1 cut(s) 669
Eco47I GGWCC 1 cut(s) 777
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 4 cut(s) 822, 1025, 1127, 1495
Eco88I CYCGRG 2 cut(s) 896, 1351
EcoICRI GAGCTC 1 cut(s) 667
EcoNI CCTNNNNNAGG 1 cut(s) 1179
EcoT14I CCWWGG 2 cut(s) 654, 920
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 2 cut(s) 654, 920
FaeI CATG 9 cut(s) 269, 335, 519, 563, 571, 658, 723, 1330, 1481
FalI AAGNNNNNCTT 4 cut(s) 1052, 1084, 1445, 1477
FaqI GGGAC 4 cut(s) 526, 552, 1333, 1405
FatI CATG 9 cut(s) 265, 331, 515, 559, 567, 654, 719, 1326, 1477
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 1 cut(s) 1336
Fnu4HI GCNGC 3 cut(s) 276, 591, 1359
FokI GGATG 4 cut(s) 29, 937, 998, 1514
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 3 cut(s) 276, 591, 1359
FspBI CTAG 4 cut(s) 236, 1148, 1413, 1472
GluI GCNGC 3 cut(s) 276, 591, 1359
HaeIII GGCC 2 cut(s) 330, 585
HapII CCGG 1 cut(s) 1532
HgaI GACGC 1 cut(s) 1057
Hin1II CATG 9 cut(s) 269, 335, 519, 563, 571, 658, 723, 1330, 1481
HindIII AAGCTT 2 cut(s) 700, 725
HpaII CCGG 1 cut(s) 1532
HphI GGTGA 2 cut(s) 142, 1283
Hpy166II GTNNAC 2 cut(s) 557, 763
Hpy188I TCNGA 9 cut(s) 206, 575, 769, 1030, 1084, 1108, 1181, 1204, 1210
Hpy188III TCNNGA 8 cut(s) 10, 211, 641, 800, 896, 1382, 1499, 1513
Hpy8I GTNNAC 2 cut(s) 557, 763
HpyAV CCTTC 4 cut(s) 56, 293, 1054, 1115
HpyCH4III ACNGT 3 cut(s) 754, 760, 1406
HpyCH4IV ACGT 1 cut(s) 810
HpyCH4V TGCA 6 cut(s) 184, 479, 590, 749, 928, 1421
HpyF10VI GCNNNNNNNGC 1 cut(s) 842
HpyF3I CTNAG 7 cut(s) 55, 175, 612, 728, 766, 1287, 1500
HpySE526I ACGT 1 cut(s) 810
Hsp92II CATG 9 cut(s) 269, 335, 519, 563, 571, 658, 723, 1330, 1481
KpnI GGTACC 1 cut(s) 567
Kzo9I GATC 4 cut(s) 6, 382, 1378, 1495
LmnI GCTCC 2 cut(s) 293, 842
Lsp1109I GCAGC 3 cut(s) 287, 602, 1345
LweI GCATC 4 cut(s) 598, 915, 1020, 1536
MaeI CTAG 4 cut(s) 236, 1148, 1413, 1472
MaeII ACGT 1 cut(s) 810
MaeIII GTNAC 5 cut(s) 244, 754, 1090, 1406, 1536
MalI GATC 4 cut(s) 8, 384, 1380, 1497
MboI GATC 4 cut(s) 6, 382, 1378, 1495
MboII GAAGA 9 cut(s) 31, 89, 180, 386, 645, 815, 1060, 1495, 1501
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 2 cut(s) 6, 1378
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 9 cut(s) 82, 121, 217, 474, 883, 934, 965, 1101, 1212
MlyI GAGTC 3 cut(s) 717, 908, 998
MmeI TCCRAC 3 cut(s) 138, 545, 962
MnlI CCTC 9 cut(s) 121, 334, 545, 677, 680, 686, 1175, 1189, 1437
MroXI GAANNNNTTC 2 cut(s) 1064, 1508
MseI TTAA 1 cut(s) 1452
MslI CAYNNNNRTG 2 cut(s) 546, 1532
MspA1I CMGCKG 3 cut(s) 278, 593, 1045
MspI CCGG 1 cut(s) 1532
MspR9I CCNGG 1 cut(s) 1532
MunI CAATTG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 842
NciI CCSGG 1 cut(s) 1532
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1336
NdeII GATC 4 cut(s) 6, 382, 1378, 1495
NlaIII CATG 9 cut(s) 269, 335, 519, 563, 571, 658, 723, 1330, 1481
NlaIV GGNNCC 3 cut(s) 329, 565, 738
NspI RCATGY 2 cut(s) 519, 1481
NspV TTCGAA 1 cut(s) 1343
PaeR7I CTCGAG 1 cut(s) 896
PciI ACATGT 2 cut(s) 515, 1477
PcsI WCGNNNNNNNCGW 3 cut(s) 894, 1055, 1349
PdmI GAANNNNTTC 2 cut(s) 1064, 1508
PfeI GAWTC 7 cut(s) 199, 207, 440, 796, 1025, 1065, 1205
PflFI GACNNNGTC 1 cut(s) 599
PflMI CCANNNNNTGG 2 cut(s) 527, 1258
PkrI GCNGC 3 cut(s) 277, 592, 1360
PleI GAGTC 3 cut(s) 716, 907, 997
PpsI GAGTC 3 cut(s) 716, 907, 997
PscI ACATGT 2 cut(s) 515, 1477
Psp124BI GAGCTC 1 cut(s) 669
PspN4I GGNNCC 3 cut(s) 329, 565, 738
PspPI GGNCC 2 cut(s) 328, 777
PstNI CAGNNNCTG 1 cut(s) 1112
PsuI RGATCY 2 cut(s) 6, 1378
PsyI GACNNNGTC 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 9 cut(s) 24, 260, 467, 565, 809, 850, 1319, 1325, 1476
RsaNI GTAC 9 cut(s) 23, 259, 466, 564, 808, 849, 1318, 1324, 1475
RseI CAYNNNNRTG 2 cut(s) 546, 1532
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 1 cut(s) 1452
SatI GCNGC 3 cut(s) 276, 591, 1359
Sau3AI GATC 4 cut(s) 6, 382, 1378, 1495
Sau96I GGNCC 2 cut(s) 328, 777
ScaI AGTACT 2 cut(s) 24, 260
SchI GAGTC 3 cut(s) 717, 908, 998
ScrFI CCNGG 1 cut(s) 1532
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 4 cut(s) 598, 915, 1020, 1536
SfcI CTRYAG 1 cut(s) 1372
Sfr274I CTCGAG 1 cut(s) 896
SfuI TTCGAA 1 cut(s) 1343
SinI GGWCC 1 cut(s) 777
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 546, 1532
SmlI CTYRAG 2 cut(s) 697, 896
SmoI CTYRAG 2 cut(s) 697, 896
SpeI ACTAGT 1 cut(s) 1471
Sse9I AATT 9 cut(s) 82, 121, 217, 474, 883, 934, 965, 1101, 1212
SsiI CCGC 3 cut(s) 278, 324, 1045
SspMI CTAG 4 cut(s) 236, 1148, 1413, 1472
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 1 cut(s) 1530
StyI CCWWGG 2 cut(s) 654, 920
TaaI ACNGT 3 cut(s) 754, 760, 1406
TaiI ACGT 1 cut(s) 813
TaqI TCGA 9 cut(s) 11, 381, 438, 621, 682, 897, 1058, 1304, 1343
TasI AATT 9 cut(s) 82, 121, 217, 474, 883, 934, 965, 1101, 1212
TatI WGTACW 5 cut(s) 22, 258, 465, 1323, 1474
TfiI GAWTC 7 cut(s) 199, 207, 440, 796, 1025, 1065, 1205
Tru1I TTAA 1 cut(s) 1452
Tru9I TTAA 1 cut(s) 1452
TseI GCWGC 3 cut(s) 275, 590, 1358
TspDTI ATGAA 4 cut(s) 31, 361, 987, 1322
TspGWI ACGGA 2 cut(s) 855, 873
Tth111I GACNNNGTC 1 cut(s) 599
Van91I CCANNNNNTGG 2 cut(s) 527, 1258
VpaK11BI GGWCC 1 cut(s) 777
XagI CCTNNNNNAGG 1 cut(s) 1179
XapI RAATTY 5 cut(s) 82, 121, 934, 965, 1212
XceI RCATGY 2 cut(s) 519, 1481
XhoI CTCGAG 1 cut(s) 896
XmnI GAANNNNTTC 2 cut(s) 1064, 1508
XspI CTAG 4 cut(s) 236, 1148, 1413, 1472
ZrmI AGTACT 2 cut(s) 24, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.