MD05G1267800.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000925
Physical Location & Seq
Forward (+)
40292424 .. 40293365
942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1267800.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGGAAAAGAACTCTTTTGGGCAATTCGAGGTGGCGGAGGCTTAAACTTCGGAGTCATCCTGGCCTGGAAACTTAGATTGGTTCCTGTTCCTCCAATTGTAACAGGCTACATGGAATCGAAGACCATTGAACAAGGTGCAACCAAACTTGTTTCCAAATGGCAAGCTATAGCTGATAGGATGCCTCAACAGTACTTCCTGCGTTTGGTTATCCAAGCTGCAAATGGAACTGGTACAAATGGTGGAAAAACTATTCAAGTTATGTTCAATTTCGTGGCTTTTGAGACTGTTGAGAAACTTCTCCCTTGGATGCAAGAAAATTTCCCTGAGTTAAATTTAGACCAGTCCACCTTCACTGAAATGAGTTGGATTGAGTCCGCTCTATACGTTGCTAACCTCCCGATAAACGAATCACAAGTTCTGCTTCAGCGGACTCAACAATCGAGGGTATTCTTCAAAAACAAATCAGATTTTGTTACTGAGCCTATTTCAGAAGTTGCCTTAGAAGGTTTATGGAAAGCAATGCTTGAATTGGATTCATTACAGTTGATGCTGACACCTTACGGTGGAAGAATGAGTGAGATTTATGATTGGGAAATTCCTTTCCCACATAGAAAAGGAAATTTGTTTGAGATCCAGTATGTCGACAATTGGGTTGATGACAAAGAAACTGAGGAACATATTAGAGGGGTGAGAAGTGTGTATGAGTACATGACACCATATGTTTCAAAGTCCCCAAGAGCTGCCTATTTGAATTATAGGGATCTTGACTTGGGGAGGAACAAGGATGCTAATACAAGCTATGCAGAAGCAAGCGCTTGGGGATTGAAGTATTTCAAGAATAACTTTAGGAGACTTGTTCAAATAAAGACTTCAGTCGATCCTGATAATTTCTTTAGAGATGAACAAAGCATCCCGGTGTTACAGTCTGCGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

314

Amino Acids

36.09

Weight (kDa)

5.9

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BBE PF08031 249 - 306 1.7e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 645
AciI CCGC 3 cut(s) 36, 378, 430
AclWI GGATC 3 cut(s) 628, 771, 875
AcsI RAATTY 4 cut(s) 319, 334, 597, 622
AcuI CTGAAG 2 cut(s) 410, 858
AfaI GTAC 3 cut(s) 194, 235, 710
AfeI AGCGCT 1 cut(s) 817
AfiI CCNNNNNNNGG 2 cut(s) 205, 566
AjnI CCWGG 2 cut(s) 60, 65
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AloI GAACNNNNNNTCC 2 cut(s) 897, 929
AluBI AGCT 5 cut(s) 167, 173, 218, 743, 801
AluI AGCT 5 cut(s) 167, 173, 218, 743, 801
Alw26I GTCTC 2 cut(s) 278, 847
AlwI GGATC 3 cut(s) 628, 771, 875
Aor51HI AGCGCT 1 cut(s) 817
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 218, 743
ApoI RAATTY 4 cut(s) 319, 334, 597, 622
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 818
AsuC2I CCSGG 1 cut(s) 917
AsuHPI GGTGA 1 cut(s) 703
BbsI GAAGAC 1 cut(s) 128
BbvI GCAGC 2 cut(s) 205, 730
BciT130I CCWGG 2 cut(s) 62, 67
BcnI CCSGG 1 cut(s) 917
BcoDI GTCTC 2 cut(s) 278, 847
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 819
BisI GCNGC 2 cut(s) 219, 744
BlsI GCNGC 2 cut(s) 220, 745
BmcAI AGTACT 1 cut(s) 194
Bme1390I CCNGG 3 cut(s) 62, 67, 917
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 3 cut(s) 62, 67, 917
BmsI GCATC 5 cut(s) 171, 300, 540, 778, 921
BoxI GACNNNNGTC 1 cut(s) 875
BpiI GAAGAC 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 917
BsaJI CCNNGG 1 cut(s) 305
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 566
Bse1I ACTGG 3 cut(s) 235, 343, 637
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 2 cut(s) 62, 67
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 5 cut(s) 57, 186, 315, 793, 912
BseLI CCNNNNNNNGG 2 cut(s) 205, 566
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 3 cut(s) 318, 471, 663
BseNI ACTGG 3 cut(s) 235, 343, 637
BseXI GCAGC 2 cut(s) 205, 730
BshFI GGCC 1 cut(s) 65
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 2 cut(s) 205, 566
BsmAI GTCTC 2 cut(s) 278, 847
BsmFI GGGAC 1 cut(s) 718
BsnI GGCC 1 cut(s) 65
Bsp143I GATC 3 cut(s) 633, 763, 880
BspACI CCGC 3 cut(s) 36, 378, 430
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 3 cut(s) 319, 472, 664
BspLI GGNNCC 1 cut(s) 84
BspPI GGATC 3 cut(s) 628, 771, 875
BsrBI CCGCTC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 3 cut(s) 235, 343, 637
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 3 cut(s) 633, 763, 880
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 2 cut(s) 62, 67
Bst4CI ACNGT 5 cut(s) 192, 289, 546, 566, 927
BstC8I GCNNGC 2 cut(s) 165, 814
BstDEI CTNAG 5 cut(s) 74, 327, 480, 502, 672
BstF5I GGATG 5 cut(s) 57, 186, 315, 793, 912
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 1 cut(s) 818
BstKTI GATC 3 cut(s) 636, 766, 883
BstMAI GTCTC 2 cut(s) 278, 847
BstMBI GATC 3 cut(s) 633, 763, 880
BstNI CCWGG 2 cut(s) 62, 67
BstPAI GACNNNNGTC 1 cut(s) 875
BstSCI CCNGG 3 cut(s) 60, 65, 915
BstSFI CTRYAG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 205, 730
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 2 cut(s) 633, 763
BstYI RGATCY 2 cut(s) 633, 763
BsuRI GGCC 1 cut(s) 65
BtsCI GGATG 5 cut(s) 57, 186, 315, 793, 912
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 2 cut(s) 165, 814
CfoI GCGC 1 cut(s) 818
Csp6I GTAC 3 cut(s) 193, 234, 709
CviAII CATG 2 cut(s) 112, 712
CviQI GTAC 3 cut(s) 193, 234, 709
DdeI CTNAG 5 cut(s) 74, 327, 480, 502, 672
DpnI GATC 3 cut(s) 635, 765, 882
DpnII GATC 3 cut(s) 633, 763, 880
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 305
Eco47III AGCGCT 1 cut(s) 817
Eco57I CTGAAG 2 cut(s) 410, 858
EcoRII CCWGG 2 cut(s) 60, 65
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 2 cut(s) 115, 715
FalI AAGNNNNNCTT 6 cut(s) 408, 440, 510, 542, 830, 862
FaqI GGGAC 1 cut(s) 718
FatI CATG 2 cut(s) 111, 711
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 2 cut(s) 219, 744
FokI GGATG 5 cut(s) 44, 193, 322, 800, 899
Fsp4HI GCNGC 2 cut(s) 219, 744
GlaI GCGC 1 cut(s) 817
GluI GCNGC 2 cut(s) 219, 744
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 1 cut(s) 65
HapII CCGG 1 cut(s) 917
HhaI GCGC 1 cut(s) 818
Hin1II CATG 2 cut(s) 115, 715
Hin6I GCGC 1 cut(s) 816
HinP1I GCGC 1 cut(s) 816
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 6 cut(s) 54, 116, 374, 410, 433, 536
HpaII CCGG 1 cut(s) 917
HphI GGTGA 1 cut(s) 703
Hpy166II GTNNAC 2 cut(s) 348, 646
Hpy188I TCNGA 3 cut(s) 53, 469, 493
Hpy188III TCNNGA 4 cut(s) 400, 767, 838, 884
Hpy8I GTNNAC 2 cut(s) 348, 646
HpyAV CCTTC 2 cut(s) 361, 500
HpyCH4III ACNGT 5 cut(s) 192, 289, 546, 566, 927
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 4 cut(s) 140, 221, 313, 806
HpyF3I CTNAG 5 cut(s) 74, 327, 480, 502, 672
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 2 cut(s) 115, 715
HspAI GCGC 1 cut(s) 816
Kzo9I GATC 3 cut(s) 633, 763, 880
Lsp1109I GCAGC 2 cut(s) 205, 730
LweI GCATC 5 cut(s) 171, 300, 540, 778, 921
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 100, 475, 921
MalI GATC 3 cut(s) 635, 765, 882
MbiI CCGCTC 1 cut(s) 380
MboI GATC 3 cut(s) 633, 763, 880
MboII GAAGA 3 cut(s) 133, 445, 582
MfeI CAATTG 2 cut(s) 96, 649
MflI RGATCY 2 cut(s) 633, 763
MlyI GAGTC 3 cut(s) 63, 383, 427
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 9 cut(s) 23, 32, 102, 195, 407, 438, 667, 680, 771
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 2 cut(s) 44, 332
MslI CAYNNNNRTG 2 cut(s) 359, 917
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 3 cut(s) 62, 67, 917
MunI CAATTG 2 cut(s) 96, 649
MvaI CCWGG 2 cut(s) 62, 67
NciI CCSGG 1 cut(s) 917
NdeI CATATG 1 cut(s) 721
NdeII GATC 3 cut(s) 633, 763, 880
NlaIII CATG 2 cut(s) 115, 715
NlaIV GGNNCC 1 cut(s) 84
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 3 cut(s) 116, 410, 536
PkrI GCNGC 2 cut(s) 220, 745
PleI GAGTC 3 cut(s) 62, 382, 427
PpsI GAGTC 3 cut(s) 62, 382, 427
PshAI GACNNNNGTC 1 cut(s) 875
Psp6I CCWGG 2 cut(s) 60, 65
PspGI CCWGG 2 cut(s) 60, 65
PspN4I GGNNCC 1 cut(s) 84
PsuI RGATCY 2 cut(s) 633, 763
RsaI GTAC 3 cut(s) 194, 235, 710
RsaNI GTAC 3 cut(s) 193, 234, 709
RseI CAYNNNNRTG 2 cut(s) 359, 917
SalI GTCGAC 1 cut(s) 644
SaqAI TTAA 2 cut(s) 44, 332
SatI GCNGC 2 cut(s) 219, 744
Sau3AI GATC 3 cut(s) 633, 763, 880
ScaI AGTACT 1 cut(s) 194
SchI GAGTC 3 cut(s) 63, 383, 427
ScrFI CCNGG 3 cut(s) 62, 67, 917
SfaNI GCATC 5 cut(s) 171, 300, 540, 778, 921
SfcI CTRYAG 1 cut(s) 168
SmiMI CAYNNNNRTG 2 cut(s) 359, 917
SsiI CCGC 3 cut(s) 36, 378, 430
StyD4I CCNGG 3 cut(s) 60, 65, 915
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 5 cut(s) 192, 289, 546, 566, 927
TaiI ACGT 1 cut(s) 390
TaqI TCGA 5 cut(s) 28, 119, 443, 645, 879
TatI WGTACW 2 cut(s) 192, 708
TfiI GAWTC 3 cut(s) 116, 410, 536
Tru1I TTAA 2 cut(s) 44, 332
Tru9I TTAA 2 cut(s) 44, 332
TscAI CASTG 1 cut(s) 361
TseI GCWGC 2 cut(s) 218, 743
TspDTI ATGAA 2 cut(s) 528, 918
TspRI CASTG 1 cut(s) 361
XapI RAATTY 4 cut(s) 319, 334, 597, 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 645
XmnI GAANNNNTTC 1 cut(s) 833
ZrmI AGTACT 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.