Rroxscaffold_1G00059800

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000925
Physical Location & Seq
Forward (+)
81887404 .. 81888984
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059800.1

Sequence Viewer

Length: 1581 bp
ATGAATATGGAGACCTTTCATGTAAAACTATTTTCCCTACTTTTCATCCTTCTCAACTCAGTTTGGTCAACAACTTCAAGATCAAATTCTGAAAGCTTTGTTCAATGCTTTTCCTCCCACCTACAGAACTCTAACTCCAGTAATGAAATCATTCTCCCCAGAACCAGTTCTGCTTATCCCTCAGTCCTAGACTTTTCCATACAGAACCTCAGATTCCTGAACAATTCCACACCTAAACCAGAGGCCATCATTACTCCTTTTGATGAATCTCAGGTTCAAGCAGCTGTAATTTGCTCCAAGAAAAGTGGCATACAAATAAGAATCCGAAGCGGGGGTCATGATTACGAGGGCCTATCTTATGTGTCTAGAGCTCCTTTCATCATCATTGACCTTTTCAATCTCCGTTCCATCGATGTTGATATAAAGAATGAGAGTGCTTGGGTCAAATCAGGAGCTACTCTTGGAGAGTTGTATTACAACATTGCACAGAAGAGTAAAGTCTATGCCTTCCCAGCCGGGACTTGTCCCACCGTAGGTGCTGGGGGACACATCAGTGGAGGTGGAATTGGTACCATATTCAGAAAATATGGTCTAGCATCCGACAATGTCATTGATGCTAGAATTGTAGATGTTAATGGCAGAATTCTAGATAGAAAGTCCATGGGAGAAGAGCTCTTTTGGGCTATCAGAGGAGGAGGAGGATCAAGCTTTGGAGTCATTCTAGCATGGAAACTTAGACTGGTTCCTGTTCCCCCAACTGTAACAGTTTGCGACGTTACAAAGACAAAAGAACAAGGTGCAACCAAACTTCTTTTGAAATGGCAAAACATTGCGGACAAGCTTCCTGAAGACCTTTTCCTACATTCAGTTGTAGGAAGTAGTGACAACACTATTACAGTTGAATTCGGTTCCCTGTTTCTTGGGCGGGTCGACAAAGCTGTCCAGGTGATACAGGAAAACTTCCCAGAGTTGAGTTTAGAGAGCAGCCATTGCAGTGAGATGAGTTGGATTCAGTCTGTTTTGGACTCTGCTGGCTACTCGATACACGAATCCTTGGAGATTTTGCTGAAGCGCCGGCAATACAGCTACTTCTTTAAAGCAAAATCAGACTTTGTGACTGAACCCATCTCAGAAGCTGGCTTGGAAGGCTTATGGCACATATTGCTTGAAGCAAACACATCCTATATGATATTGACACCATATGGAGGAAAAATGAGTGAGATTTCCGATTCAGAAACTGCTTTCCCACATCGAAAAGGAAACAAATACGAAATCCAGTATATGGTTACTTGGGGTGACGGCAAGGACACCAAGAAGTATGTTGGATTTATGAGAAGGCTGTATGCATACATGGCCCCGTATGTTTCAAAGTCTCCTAGAGCTGCCTATTTGAATTATAGGGATCTTGATTTGGGGAGGAACAGTTATGGCAATACAAGCTATGCACAAGCAAGCATTTGGGGTTTGAAGTACTTCAAGAACAACTTTAGGAGACTTGTTCATGTTAAGACCTTGGTTGATCCTGGCAACTTCTTTAGGAATGAGCAAAGCATCCCCGTGTTTTCGTCAGGAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

526

Amino Acids

58.8

Weight (kDa)

8.88

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 80 - 216 9.5e-25 FAD binding domain
BBE PF08031 462 - 519 2.2e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 938
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 1 cut(s) 569
AccB7I CCANNNNNTGG 1 cut(s) 1282
AccI GTMKAC 1 cut(s) 930
AciI CCGC 3 cut(s) 330, 833, 925
AclWI GGATC 3 cut(s) 709, 1410, 1514
AcsI RAATTY 3 cut(s) 85, 642, 902
AcuI CTGAAG 2 cut(s) 867, 1088
AfaI GTAC 2 cut(s) 571, 1472
AfiI CCNNNNNNNGG 4 cut(s) 331, 533, 1205, 1282
AjnI CCWGG 2 cut(s) 942, 1522
AleI CACNNNNGTG 1 cut(s) 552
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
Alw21I GWGCWC 2 cut(s) 373, 675
Alw26I GTCTC 3 cut(s) 5, 1377, 1486
AlwI GGATC 3 cut(s) 709, 1410, 1514
AlwNI CAGNNNCTG 2 cut(s) 1136, 1238
AoxI GGCC 3 cut(s) 243, 349, 1353
ApeKI GCWGC 3 cut(s) 281, 984, 1382
ApoI RAATTY 3 cut(s) 85, 642, 902
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
Asp700I GAANNNNTTC 3 cut(s) 150, 166, 1472
Asp718I GGTACC 1 cut(s) 569
AspLEI GCGC 1 cut(s) 1074
AspS9I GGNCC 2 cut(s) 349, 1354
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 2 cut(s) 958, 1307
BanI GGYRCC 1 cut(s) 569
BanII GRGCYC 2 cut(s) 373, 675
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 373, 675
BbvI GCAGC 3 cut(s) 293, 996, 1369
BccI CCATC 3 cut(s) 254, 416, 1133
BceAI ACGGC 1 cut(s) 1315
BciT130I CCWGG 2 cut(s) 944, 1524
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 3 cut(s) 5, 1377, 1486
BfaI CTAG 7 cut(s) 188, 366, 593, 618, 647, 722, 1377
BfmI CTRYAG 1 cut(s) 122
BfoI RGCGCY 1 cut(s) 1075
BisI GCNGC 3 cut(s) 282, 985, 1383
BlsI GCNGC 3 cut(s) 283, 986, 1384
BmcAI AGTACT 1 cut(s) 1472
Bme1390I CCNGG 3 cut(s) 517, 944, 1524
BmgT120I GGNCC 2 cut(s) 349, 1354
BmiI GGNNCC 4 cut(s) 571, 744, 910, 1356
BmrFI CCNGG 3 cut(s) 517, 944, 1524
BmsI GCATC 3 cut(s) 604, 605, 1560
BpiI GAAGAC 1 cut(s) 855
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 1 cut(s) 121
BpuMI CCSGG 1 cut(s) 517
Bsa29I ATCGAT 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 660, 1053, 1512
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 1408, 1438
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 533, 1205, 1282
Bse118I RCCGGY 1 cut(s) 1074
Bse1I ACTGG 4 cut(s) 138, 165, 744, 1276
Bse3DI GCAATG 3 cut(s) 480, 828, 988
BseBI CCWGG 2 cut(s) 944, 1524
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 660, 1053, 1512
BseGI GGATG 4 cut(s) 45, 596, 1178, 1551
BseLI CCNNNNNNNGG 4 cut(s) 331, 533, 1205, 1282
BseMI GCAATG 3 cut(s) 480, 828, 988
BseMII CTCAG 5 cut(s) 72, 195, 223, 284, 1143
BseNI ACTGG 4 cut(s) 138, 165, 744, 1276
BseRI GAGGAG 3 cut(s) 705, 708, 711
BseXI GCAGC 3 cut(s) 293, 996, 1369
BseYI CCCAGC 2 cut(s) 511, 539
BshFI GGCC 3 cut(s) 245, 351, 1355
BshNI GGYRCC 1 cut(s) 569
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 373, 675
BsiSI CCGG 2 cut(s) 516, 1075
BslFI GGGAC 3 cut(s) 510, 532, 558
BslI CCNNNNNNNGG 4 cut(s) 331, 533, 1205, 1282
BsmAI GTCTC 3 cut(s) 5, 1377, 1486
BsmFI GGGAC 3 cut(s) 510, 532, 558
BsnI GGCC 3 cut(s) 245, 351, 1355
Bso31I GGTCTC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 373, 675
Bsp143I GATC 4 cut(s) 80, 701, 1402, 1519
Bsp19I CCATGG 1 cut(s) 660
BspACI CCGC 3 cut(s) 330, 833, 925
BspANI GGCC 3 cut(s) 245, 351, 1355
BspCNI CTCAG 5 cut(s) 71, 194, 222, 283, 1142
BspDI ATCGAT 1 cut(s) 411
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 4 cut(s) 571, 744, 910, 1356
BspPI GGATC 3 cut(s) 709, 1410, 1514
BspQI GCTCTTC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 5
BsrDI GCAATG 3 cut(s) 480, 828, 988
BsrFI RCCGGY 1 cut(s) 1074
BsrI ACTGG 4 cut(s) 138, 165, 744, 1276
BssAI RCCGGY 1 cut(s) 1074
BssECI CCNNGG 3 cut(s) 660, 1053, 1512
BssMI GATC 4 cut(s) 80, 701, 1402, 1519
BssT1I CCWWGG 3 cut(s) 660, 1053, 1512
Bst2UI CCWGG 2 cut(s) 944, 1524
Bst4CI ACNGT 5 cut(s) 532, 760, 766, 898, 1424
Bst6I CTCTTC 2 cut(s) 485, 663
BstAPI GCANNNNNTGC 2 cut(s) 990, 1162
BstC8I GCNNGC 4 cut(s) 1033, 1076, 1138, 1453
BstDEI CTNAG 6 cut(s) 58, 181, 209, 270, 734, 1129
BstDSI CCRYGG 1 cut(s) 660
BstF5I GGATG 4 cut(s) 45, 596, 1178, 1551
BstH2I RGCGCY 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 1074
BstKTI GATC 4 cut(s) 83, 704, 1405, 1522
BstMAI GTCTC 3 cut(s) 5, 1377, 1486
BstMBI GATC 4 cut(s) 80, 701, 1402, 1519
BstMWI GCNNNNNNNGC 6 cut(s) 512, 990, 1146, 1162, 1352, 1437
BstNI CCWGG 2 cut(s) 944, 1524
BstSCI CCNGG 3 cut(s) 515, 942, 1522
BstSFI CTRYAG 1 cut(s) 122
BstV1I GCAGC 3 cut(s) 293, 996, 1369
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 1402
BstYI RGATCY 1 cut(s) 1402
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 3 cut(s) 245, 351, 1355
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 1 cut(s) 660
BtsCI GGATG 4 cut(s) 45, 596, 1178, 1551
BtsI GCAGTG 1 cut(s) 1000
BtsIMutI CAGTG 2 cut(s) 559, 1000
Cac8I GCNNGC 4 cut(s) 1033, 1076, 1138, 1453
CaiI CAGNNNCTG 2 cut(s) 1136, 1238
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 1074
Cfr10I RCCGGY 1 cut(s) 1074
Cfr13I GGNCC 2 cut(s) 349, 1354
ClaI ATCGAT 1 cut(s) 411
Csp6I GTAC 2 cut(s) 570, 1471
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 6 cut(s) 20, 338, 661, 726, 1351, 1502
CviQI GTAC 2 cut(s) 570, 1471
DdeI CTNAG 6 cut(s) 58, 181, 209, 270, 734, 1129
DpnI GATC 4 cut(s) 82, 703, 1404, 1521
DpnII GATC 4 cut(s) 80, 701, 1402, 1519
DraI TTTAAA 1 cut(s) 1096
DrdI GACNNNNNNGTC 1 cut(s) 938
DseDI GACNNNNNNGTC 1 cut(s) 938
Eam1104I CTCTTC 2 cut(s) 485, 663
EarI CTCTTC 2 cut(s) 485, 663
Ecl136II GAGCTC 2 cut(s) 371, 673
Eco130I CCWWGG 3 cut(s) 660, 1053, 1512
Eco24I GRGCYC 2 cut(s) 373, 675
Eco31I GGTCTC 1 cut(s) 5
Eco53kI GAGCTC 2 cut(s) 371, 673
Eco57I CTGAAG 2 cut(s) 867, 1088
EcoICRI GAGCTC 2 cut(s) 371, 673
EcoO109I RGGNCCY 1 cut(s) 349
EcoRI GAATTC 2 cut(s) 642, 902
EcoRII CCWGG 2 cut(s) 942, 1522
EcoT14I CCWWGG 3 cut(s) 660, 1053, 1512
EcoT22I ATGCAT 1 cut(s) 1348
EcoT38I GRGCYC 2 cut(s) 373, 675
ErhI CCWWGG 3 cut(s) 660, 1053, 1512
FaeI CATG 6 cut(s) 23, 341, 664, 729, 1354, 1505
FalI AAGNNNNNCTT 2 cut(s) 1469, 1501
FaqI GGGAC 3 cut(s) 510, 532, 558
FatI CATG 6 cut(s) 19, 337, 660, 725, 1350, 1501
FauI CCCGC 2 cut(s) 323, 918
FauNDI CATATG 1 cut(s) 1201
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 3 cut(s) 282, 985, 1383
FokI GGATG 4 cut(s) 32, 583, 1165, 1538
FriOI GRGCYC 2 cut(s) 373, 675
Fsp4HI GCNGC 3 cut(s) 282, 985, 1383
FspBI CTAG 7 cut(s) 188, 366, 593, 618, 647, 722, 1377
GlaI GCGC 1 cut(s) 1073
GluI GCNGC 3 cut(s) 282, 985, 1383
GsaI CCCAGC 2 cut(s) 515, 543
GsuI CTGGAG 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 1075
HaeIII GGCC 3 cut(s) 245, 351, 1355
HapII CCGG 2 cut(s) 516, 1075
HhaI GCGC 1 cut(s) 1074
Hin1II CATG 6 cut(s) 23, 341, 664, 729, 1354, 1505
Hin6I GCGC 1 cut(s) 1072
HinP1I GCGC 1 cut(s) 1072
HincII GTYRAC 2 cut(s) 69, 931
HindII GTYRAC 2 cut(s) 69, 931
HindIII AAGCTT 3 cut(s) 94, 706, 839
HinfI GANTC 8 cut(s) 213, 266, 321, 714, 1009, 1025, 1049, 1229
HpaII CCGG 2 cut(s) 516, 1075
HphI GGTGA 2 cut(s) 958, 1307
Hpy166II GTNNAC 2 cut(s) 69, 931
Hpy8I GTNNAC 2 cut(s) 69, 931
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 4 cut(s) 59, 517, 1139, 1329
HpyCH4III ACNGT 5 cut(s) 532, 760, 766, 898, 1424
HpyCH4IV ACGT 1 cut(s) 774
HpyCH4V TGCA 5 cut(s) 485, 800, 993, 1346, 1445
HpyF10VI GCNNNNNNNGC 6 cut(s) 512, 990, 1146, 1162, 1352, 1437
HpyF3I CTNAG 6 cut(s) 58, 181, 209, 270, 734, 1129
HpySE526I ACGT 1 cut(s) 774
Hsp92II CATG 6 cut(s) 23, 341, 664, 729, 1354, 1505
HspAI GCGC 1 cut(s) 1072
KpnI GGTACC 1 cut(s) 573
KroI GCCGGC 1 cut(s) 1074
KroNI GCCGGC 1 cut(s) 1076
Kzo9I GATC 4 cut(s) 80, 701, 1402, 1519
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 3 cut(s) 299, 376, 452
Lsp1109I GCAGC 3 cut(s) 293, 996, 1369
LweI GCATC 3 cut(s) 604, 605, 1560
MaeI CTAG 7 cut(s) 188, 366, 593, 618, 647, 722, 1377
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 6 cut(s) 760, 775, 881, 1114, 1285, 1295
MalI GATC 4 cut(s) 82, 703, 1404, 1521
MboI GATC 4 cut(s) 80, 701, 1402, 1519
MboII GAAGA 3 cut(s) 502, 680, 860
MflI RGATCY 1 cut(s) 1402
MhlI GDGCHC 2 cut(s) 373, 675
MluCI AATT 8 cut(s) 85, 223, 288, 564, 621, 642, 902, 1393
MlyI GAGTC 2 cut(s) 723, 1019
MmeI TCCRAC 3 cut(s) 624, 986, 1303
Mph1103I ATGCAT 1 cut(s) 1348
MroNI GCCGGC 1 cut(s) 1074
MroXI GAANNNNTTC 3 cut(s) 150, 166, 1472
MseI TTAA 3 cut(s) 633, 1095, 1506
MslI CAYNNNNRTG 3 cut(s) 552, 993, 1556
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 2 cut(s) 516, 1075
MspR9I CCNGG 3 cut(s) 517, 944, 1524
MvaI CCWGG 2 cut(s) 944, 1524
MwoI GCNNNNNNNGC 6 cut(s) 512, 990, 1146, 1162, 1352, 1437
NaeI GCCGGC 1 cut(s) 1076
NciI CCSGG 1 cut(s) 517
NcoI CCATGG 1 cut(s) 660
NdeI CATATG 1 cut(s) 1201
NdeII GATC 4 cut(s) 80, 701, 1402, 1519
NgoMIV GCCGGC 1 cut(s) 1074
NlaIII CATG 6 cut(s) 23, 341, 664, 729, 1354, 1505
NlaIV GGNNCC 4 cut(s) 571, 744, 910, 1356
NmuCI GTSAC 3 cut(s) 881, 1114, 1295
NsiI ATGCAT 1 cut(s) 1348
OliI CACNNNNGTG 1 cut(s) 552
PagI TCATGA 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 663
PdiI GCCGGC 1 cut(s) 1076
PdmI GAANNNNTTC 3 cut(s) 150, 166, 1472
PfeI GAWTC 6 cut(s) 213, 266, 321, 1009, 1049, 1229
PflFI GACNNNGTC 1 cut(s) 605
PflMI CCANNNNNTGG 1 cut(s) 1282
PkrI GCNGC 3 cut(s) 283, 986, 1384
PleI GAGTC 2 cut(s) 722, 1019
PpsI GAGTC 2 cut(s) 722, 1019
Psp124BI GAGCTC 2 cut(s) 373, 675
Psp6I CCWGG 2 cut(s) 942, 1522
PspFI CCCAGC 2 cut(s) 511, 539
PspGI CCWGG 2 cut(s) 942, 1522
PspN4I GGNNCC 4 cut(s) 571, 744, 910, 1356
PspPI GGNCC 2 cut(s) 349, 1354
PstNI CAGNNNCTG 2 cut(s) 1136, 1238
PsuI RGATCY 1 cut(s) 1402
PsyI GACNNNGTC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 2 cut(s) 571, 1472
RsaNI GTAC 2 cut(s) 570, 1471
RseI CAYNNNNRTG 3 cut(s) 552, 993, 1556
SacI GAGCTC 2 cut(s) 373, 675
SalI GTCGAC 1 cut(s) 929
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 3 cut(s) 633, 1095, 1506
SatI GCNGC 3 cut(s) 282, 985, 1383
Sau3AI GATC 4 cut(s) 80, 701, 1402, 1519
Sau96I GGNCC 2 cut(s) 349, 1354
ScaI AGTACT 1 cut(s) 1472
SchI GAGTC 2 cut(s) 723, 1019
ScrFI CCNGG 3 cut(s) 517, 944, 1524
SduI GDGCHC 2 cut(s) 373, 675
SfaNI GCATC 3 cut(s) 604, 605, 1560
SfcI CTRYAG 1 cut(s) 122
SmiMI CAYNNNNRTG 3 cut(s) 552, 993, 1556
Sse9I AATT 8 cut(s) 85, 223, 288, 564, 621, 642, 902, 1393
SsiI CCGC 3 cut(s) 330, 833, 925
SspMI CTAG 7 cut(s) 188, 366, 593, 618, 647, 722, 1377
SstI GAGCTC 2 cut(s) 373, 675
StyD4I CCNGG 3 cut(s) 515, 942, 1522
StyI CCWWGG 3 cut(s) 660, 1053, 1512
TaaI ACNGT 5 cut(s) 532, 760, 766, 898, 1424
TaiI ACGT 1 cut(s) 777
TaqI TCGA 4 cut(s) 411, 930, 1040, 1252
TasI AATT 8 cut(s) 85, 223, 288, 564, 621, 642, 902, 1393
TatI WGTACW 1 cut(s) 1470
TfiI GAWTC 6 cut(s) 213, 266, 321, 1009, 1049, 1229
Tru1I TTAA 3 cut(s) 633, 1095, 1506
Tru9I TTAA 3 cut(s) 633, 1095, 1506
TscAI CASTG 2 cut(s) 559, 1000
TseFI GTSAC 3 cut(s) 881, 1114, 1295
TseI GCWGC 3 cut(s) 281, 984, 1382
Tsp45I GTSAC 3 cut(s) 881, 1114, 1295
TspDTI ATGAA 7 cut(s) 8, 17, 34, 159, 279, 367, 1490
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 2 cut(s) 559, 1000
Tth111I GACNNNGTC 1 cut(s) 605
Van91I CCANNNNNTGG 1 cut(s) 1282
XapI RAATTY 3 cut(s) 85, 642, 902
XbaI TCTAGA 2 cut(s) 365, 646
XmiI GTMKAC 1 cut(s) 930
XmnI GAANNNNTTC 3 cut(s) 150, 166, 1472
XspI CTAG 7 cut(s) 188, 366, 593, 618, 647, 722, 1377
ZrmI AGTACT 1 cut(s) 1472
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.