Prupe.4G096700_v2.0.a1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000925
Physical Location & Seq
Reverse (-)
4855313 .. 4857119
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G096700.1

Sequence Viewer

Length: 1593 bp
ATGAATATGGAGATATTCAAACTACTTCCAATACTTTTCATCTTTATCAACCCTGTTTGGTCCATAACTTCAAATTCAGCTACAGAAAGCTTTCTTCAATGTTTTACCTCCCACATACAGAATCTCAAATCAAGTAGCAAAATCATTCTCATCAAAAACAGTTCTGCTTATGCCTCAGTCGTGCAATCTTCCATACAAAACCTCAGATTCTTGAACACTTCAATATCAAAACCAGAAGCTGTCATTTATCCCTTTCATGACTCTCATGTTCAAGCAGCTGTAATTTGCTCCAAGACAAACGGCATACTGATAAGAATCCGAAGCGGGGGCCATGACTACGAGGGCCAATCTTACATATCCAGAGCTCCTTTTGTCATCATTGATCTTTCCAATCTTCGGTCTATTGATGTTGACATAGAAAATGAGAGTGCTTGGGTTGAATCCGGTGCTACTCTTGGAGAGTTGTATTACAGAATTGCAGAAAAAAGTAAGGCTTATGGATTTCCAGCAGGGTCATGTCCTACTGTGGGTGTGGGGGGGCTTATTAGTGGAGGTGGATTTGGTACCTTGTTCAGGAAGTATGGTCTGGCAGCTGACAATGTCCTTGATGCTAAAATTGTCGACGTTAATGGCAGAGTTCTTGACAGAAAATCCATGGGAGAAGAACTCTTTTGGGCTATTAGAGGAGGGGGAGGTTCAAGCTTCGGAGTTATTGTGGCATGGAAACTTAGGTTGGTTCCTGTCCCTCCATCTGTAACAGTTTTCTCAATTTCAAAGACCACAGAACAAGGTGCAACCAAACTTCTTTCTAAATGGCAAAACATTGCAGACAAGTTTCATGAAGACCTTTTCCTAGATACAGTCATAGGTGTTGGAGATAAAGCTGGCACAAATGGTGGCAAAACTATTGTTATTGAATTCCGTTCATTGTTTCTTGGGCCGGTGGAGAAACTTCTCCTTTTGATGCAGGACAATTTTCCAGAGTTAAGTGTAGCCCGCAGCGATTGCACTGAAATGAGTTGGATCGAGTCTGTTCTTTACTTTGCTAGCATCTCAATAAACGAATCAGAAGCTTTGCTCAATAGGACTCAACAATCTAAGAGCTTCTTCAAAGCAAAATCAGACTATGTCAATGAACCTATTTCAGAAGCTGGCTTGGAAGGTTTATGGCAAAACCTAATTGAAGTAGGGGCTTACCTCATATTGACACCTTATGGCGGAAAAATGAGTGAGATTTCCAATTCAGAAATTCCTTTCCCACACAGAAGTGGAAATCTATTCAAAATCCAGTATATGGTCACTTGGGATGATGAGAAGGAAACTGAGAAACATATTGGCATGATGAGAAAGCTGTATTCCTACATGGCACCATATGTTTCAAAGTCCCCAAGAGCTGCCTATTTGAACTACAAGGATCTTGACTTGGGGAGGAACAAGGATGCTAATACAAGCTATGCACAAGCAAGCATTTGGGGTTTCAGTTACTTCAAGAATAACTTCAGGAGACTTGCTCAAGTGAAGACCTTAGTTGATCCTGGTAACTTCTTCAGGGATGAACAAAGCATCCCTGTGTATCCATCTAGGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

531

Amino Acids

59.01

Weight (kDa)

8.38

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 563, 1366
AccB7I CCANNNNNTGG 1 cut(s) 1294
AccI GTMKAC 1 cut(s) 621
AciI CCGC 3 cut(s) 324, 997, 1218
AclWI GGATC 3 cut(s) 1031, 1422, 1526
AcsI RAATTY 3 cut(s) 73, 917, 1248
AcuI CTGAAG 2 cut(s) 1483, 1531
AfaI GTAC 1 cut(s) 565
AfiI CCNNNNNNNGG 6 cut(s) 325, 396, 527, 573, 1217, 1294
AjnI CCWGG 1 cut(s) 1534
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AleI CACNNNNGTG 1 cut(s) 1266
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 1 cut(s) 1498
AlwI GGATC 3 cut(s) 1031, 1422, 1526
AlwNI CAGNNNCTG 2 cut(s) 239, 1151
AoxI GGCC 3 cut(s) 328, 343, 938
ApeKI GCWGC 4 cut(s) 275, 590, 999, 1394
ApoI RAATTY 3 cut(s) 73, 917, 1248
Asp700I GAANNNNTTC 2 cut(s) 90, 1496
Asp718I GGTACC 1 cut(s) 563
AspS9I GGNCC 4 cut(s) 60, 328, 343, 938
AsuNHI GCTAGC 1 cut(s) 1046
AvaII GGWCC 1 cut(s) 60
BanI GGYRCC 2 cut(s) 563, 1366
BanII GRGCYC 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 849, 1526
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 4 cut(s) 287, 602, 1011, 1381
BccI CCATC 2 cut(s) 757, 1585
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 1536
BciVI GTATCC 1 cut(s) 1584
BcoDI GTCTC 1 cut(s) 1498
BfaI CTAG 3 cut(s) 854, 1047, 1581
BfmI CTRYAG 1 cut(s) 81
BfuI GTATCC 1 cut(s) 1584
BisI GCNGC 4 cut(s) 276, 591, 1000, 1395
BlsI GCNGC 4 cut(s) 277, 592, 1001, 1396
Bme1390I CCNGG 1 cut(s) 1536
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 4 cut(s) 60, 328, 343, 938
BmiI GGNNCC 4 cut(s) 329, 565, 738, 1368
BmrFI CCNGG 1 cut(s) 1536
BmsI GCATC 5 cut(s) 598, 954, 1059, 1429, 1572
BmtI GCTAGC 1 cut(s) 1050
BpiI GAAGAC 2 cut(s) 849, 1526
BplI GAGNNNNNCTC 4 cut(s) 651, 683, 1495, 1527
BpuEI CTTGAG 1 cut(s) 1497
BsaBI GATNNNNATC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 654
BsaWI WCCGGW 1 cut(s) 443
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 6 cut(s) 325, 396, 527, 573, 1217, 1294
Bse118I RCCGGY 1 cut(s) 940
Bse1I ACTGG 1 cut(s) 1288
Bse3DI GCAATG 1 cut(s) 822
Bse8I GATNNNNATC 1 cut(s) 314
BseBI CCWGG 1 cut(s) 1536
BseDI CCNNGG 1 cut(s) 654
BseGI GGATG 4 cut(s) 1312, 1444, 1558, 1563
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 6 cut(s) 325, 396, 527, 573, 1217, 1294
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 3 cut(s) 189, 217, 1314
BseNI ACTGG 1 cut(s) 1288
BseRI GAGGAG 1 cut(s) 699
BseXI GCAGC 4 cut(s) 287, 602, 1011, 1381
BshFI GGCC 3 cut(s) 330, 345, 940
BshNI GGYRCC 2 cut(s) 563, 1366
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 2 cut(s) 444, 941
BslFI GGGAC 2 cut(s) 728, 1369
BslI CCNNNNNNNGG 6 cut(s) 325, 396, 527, 573, 1217, 1294
BsmAI GTCTC 1 cut(s) 1498
BsmFI GGGAC 2 cut(s) 728, 1369
BsnI GGCC 3 cut(s) 330, 345, 940
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 4 cut(s) 382, 1023, 1414, 1531
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 3 cut(s) 324, 997, 1218
BspANI GGCC 3 cut(s) 330, 345, 940
BspCNI CTCAG 3 cut(s) 188, 216, 1315
BspHI TCATGA 2 cut(s) 256, 838
BspLI GGNNCC 4 cut(s) 329, 565, 738, 1368
BspOI GCTAGC 1 cut(s) 1050
BspPI GGATC 3 cut(s) 1031, 1422, 1526
BspT107I GGYRCC 2 cut(s) 563, 1366
BsrDI GCAATG 1 cut(s) 822
BsrFI RCCGGY 1 cut(s) 940
BsrI ACTGG 1 cut(s) 1288
BssAI RCCGGY 1 cut(s) 940
BssECI CCNNGG 1 cut(s) 654
BssMI GATC 4 cut(s) 382, 1023, 1414, 1531
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 1536
Bst4CI ACNGT 4 cut(s) 161, 526, 760, 862
BstAPI GCANNNNNTGC 1 cut(s) 1005
BstC8I GCNNGC 5 cut(s) 886, 997, 1048, 1153, 1465
BstDEI CTNAG 6 cut(s) 175, 203, 728, 1098, 1323, 1525
BstDSI CCRYGG 1 cut(s) 654
BstENI CCTNNNNNAGG 1 cut(s) 571
BstF5I GGATG 4 cut(s) 1312, 1444, 1558, 1563
BstKTI GATC 4 cut(s) 385, 1026, 1417, 1534
BstMAI GTCTC 1 cut(s) 1498
BstMBI GATC 4 cut(s) 382, 1023, 1414, 1531
BstMWI GCNNNNNNNGC 1 cut(s) 1005
BstNI CCWGG 1 cut(s) 1536
BstSCI CCNGG 1 cut(s) 1534
BstSFI CTRYAG 1 cut(s) 81
BstV1I GCAGC 4 cut(s) 287, 602, 1011, 1381
BstV2I GAAGAC 2 cut(s) 849, 1526
BstX2I RGATCY 1 cut(s) 1414
BstYI RGATCY 1 cut(s) 1414
BsuI GTATCC 1 cut(s) 1584
BsuRI GGCC 3 cut(s) 330, 345, 940
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 1312, 1444, 1558, 1563
BtsIMutI CAGTG 1 cut(s) 1008
Cac8I GCNNGC 5 cut(s) 886, 997, 1048, 1153, 1465
CaiI CAGNNNCTG 2 cut(s) 239, 1151
CciI TCATGA 2 cut(s) 256, 838
Cfr10I RCCGGY 1 cut(s) 940
Cfr13I GGNCC 4 cut(s) 60, 328, 343, 938
Csp6I GTAC 1 cut(s) 564
CviAII CATG 9 cut(s) 257, 266, 332, 516, 655, 720, 839, 1339, 1363
CviQI GTAC 1 cut(s) 564
DdeI CTNAG 6 cut(s) 175, 203, 728, 1098, 1323, 1525
DpnI GATC 4 cut(s) 384, 1025, 1416, 1533
DpnII GATC 4 cut(s) 382, 1023, 1414, 1531
EciI GGCGGA 1 cut(s) 1233
Ecl136II GAGCTC 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 654
Eco24I GRGCYC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 1483, 1531
EcoICRI GAGCTC 1 cut(s) 365
EcoNI CCTNNNNNAGG 1 cut(s) 571
EcoRI GAATTC 1 cut(s) 917
EcoRII CCWGG 1 cut(s) 1534
EcoT14I CCWWGG 1 cut(s) 654
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 9 cut(s) 260, 269, 335, 519, 658, 723, 842, 1342, 1366
FalI AAGNNNNNCTT 6 cut(s) 478, 510, 1091, 1123, 1481, 1513
FaqI GGGAC 2 cut(s) 728, 1369
FatI CATG 9 cut(s) 256, 265, 331, 515, 654, 719, 838, 1338, 1362
FauI CCCGC 2 cut(s) 317, 1004
FauNDI CATATG 1 cut(s) 1372
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 4 cut(s) 276, 591, 1000, 1395
FokI GGATG 4 cut(s) 1319, 1451, 1550, 1565
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 4 cut(s) 276, 591, 1000, 1395
FspBI CTAG 3 cut(s) 854, 1047, 1581
GluI GCNGC 4 cut(s) 276, 591, 1000, 1395
HaeIII GGCC 3 cut(s) 330, 345, 940
HapII CCGG 2 cut(s) 444, 941
Hin1II CATG 9 cut(s) 260, 269, 335, 519, 658, 723, 842, 1342, 1366
HincII GTYRAC 2 cut(s) 412, 622
HindII GTYRAC 2 cut(s) 412, 622
HindIII AAGCTT 3 cut(s) 88, 700, 1071
HinfI GANTC 8 cut(s) 121, 207, 260, 315, 440, 1028, 1064, 1087
HpaII CCGG 2 cut(s) 444, 941
Hpy166II GTNNAC 2 cut(s) 412, 622
Hpy188I TCNGA 7 cut(s) 206, 320, 707, 1069, 1123, 1147, 1246
Hpy8I GTNNAC 2 cut(s) 412, 622
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 2 cut(s) 1154, 1309
HpyCH4III ACNGT 4 cut(s) 161, 526, 760, 862
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 7 cut(s) 184, 479, 794, 827, 967, 1008, 1457
HpyF10VI GCNNNNNNNGC 1 cut(s) 1005
HpyF3I CTNAG 6 cut(s) 175, 203, 728, 1098, 1323, 1525
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 9 cut(s) 260, 269, 335, 519, 658, 723, 842, 1342, 1366
KpnI GGTACC 1 cut(s) 567
Kzo9I GATC 4 cut(s) 382, 1023, 1414, 1531
LmnI GCTCC 2 cut(s) 293, 370
Lsp1109I GCAGC 4 cut(s) 287, 602, 1011, 1381
LweI GCATC 5 cut(s) 598, 954, 1059, 1429, 1572
MaeI CTAG 3 cut(s) 854, 1047, 1581
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 4 cut(s) 754, 1297, 1481, 1538
MalI GATC 4 cut(s) 384, 1025, 1416, 1533
MboI GATC 4 cut(s) 382, 1023, 1414, 1531
MboII GAAGA 8 cut(s) 86, 180, 386, 674, 854, 1099, 1531, 1537
MflI RGATCY 1 cut(s) 1414
MhlI GDGCHC 1 cut(s) 367
MlyI GAGTC 3 cut(s) 254, 1037, 1081
MmeI TCCRAC 2 cut(s) 853, 1001
MroXI GAANNNNTTC 2 cut(s) 90, 1496
MseI TTAA 2 cut(s) 627, 986
MslI CAYNNNNRTG 3 cut(s) 1013, 1266, 1568
MspA1I CMGCKG 2 cut(s) 278, 593
MspI CCGG 2 cut(s) 444, 941
MspR9I CCNGG 1 cut(s) 1536
MvaI CCWGG 1 cut(s) 1536
MwoI GCNNNNNNNGC 1 cut(s) 1005
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1372
NdeII GATC 4 cut(s) 382, 1023, 1414, 1531
NheI GCTAGC 1 cut(s) 1046
NlaIII CATG 9 cut(s) 260, 269, 335, 519, 658, 723, 842, 1342, 1366
NlaIV GGNNCC 4 cut(s) 329, 565, 738, 1368
NmuCI GTSAC 1 cut(s) 1297
OliI CACNNNNGTG 1 cut(s) 1266
PagI TCATGA 2 cut(s) 256, 838
PdmI GAANNNNTTC 2 cut(s) 90, 1496
PfeI GAWTC 5 cut(s) 121, 207, 315, 440, 1064
PflFI GACNNNGTC 2 cut(s) 599, 1127
PflMI CCANNNNNTGG 1 cut(s) 1294
PkrI GCNGC 4 cut(s) 277, 592, 1001, 1396
PleI GAGTC 3 cut(s) 254, 1036, 1081
PpsI GAGTC 3 cut(s) 254, 1036, 1081
Psp124BI GAGCTC 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 1534
PspGI CCWGG 1 cut(s) 1534
PspN4I GGNNCC 4 cut(s) 329, 565, 738, 1368
PspPI GGNCC 4 cut(s) 60, 328, 343, 938
PstNI CAGNNNCTG 2 cut(s) 239, 1151
PsuI RGATCY 1 cut(s) 1414
PsyI GACNNNGTC 2 cut(s) 599, 1127
PvuII CAGCTG 2 cut(s) 278, 593
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
RseI CAYNNNNRTG 3 cut(s) 1013, 1266, 1568
SacI GAGCTC 1 cut(s) 367
SalI GTCGAC 1 cut(s) 620
SaqAI TTAA 2 cut(s) 627, 986
SatI GCNGC 4 cut(s) 276, 591, 1000, 1395
Sau3AI GATC 4 cut(s) 382, 1023, 1414, 1531
Sau96I GGNCC 4 cut(s) 60, 328, 343, 938
SchI GAGTC 3 cut(s) 254, 1037, 1081
ScrFI CCNGG 1 cut(s) 1536
SduI GDGCHC 1 cut(s) 367
SfaNI GCATC 5 cut(s) 598, 954, 1059, 1429, 1572
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 3 cut(s) 1013, 1266, 1568
SmlI CTYRAG 1 cut(s) 1512
SmoI CTYRAG 1 cut(s) 1512
SsiI CCGC 3 cut(s) 324, 997, 1218
SspMI CTAG 3 cut(s) 854, 1047, 1581
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 1 cut(s) 1534
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 4 cut(s) 161, 526, 760, 862
TaiI ACGT 1 cut(s) 627
TaqI TCGA 2 cut(s) 621, 1026
TaqII GACCGA 1 cut(s) 387
TfiI GAWTC 5 cut(s) 121, 207, 315, 440, 1064
Tru1I TTAA 2 cut(s) 627, 986
Tru9I TTAA 2 cut(s) 627, 986
TscAI CASTG 1 cut(s) 1015
TseFI GTSAC 1 cut(s) 1297
TseI GCWGC 4 cut(s) 275, 590, 999, 1394
Tsp45I GTSAC 1 cut(s) 1297
TspDTI ATGAA 8 cut(s) 17, 28, 245, 827, 855, 915, 1149, 1569
TspGWI ACGGA 1 cut(s) 911
TspRI CASTG 1 cut(s) 1015
Tth111I GACNNNGTC 2 cut(s) 599, 1127
Van91I CCANNNNNTGG 1 cut(s) 1294
VpaK11BI GGWCC 1 cut(s) 60
XagI CCTNNNNNAGG 1 cut(s) 571
XapI RAATTY 3 cut(s) 73, 917, 1248
XmiI GTMKAC 1 cut(s) 621
XmnI GAANNNNTTC 2 cut(s) 90, 1496
XspI CTAG 3 cut(s) 854, 1047, 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.