MD05G1267900.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000925
Physical Location & Seq
Forward (+)
40310860 .. 40312455
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1267900.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGGAGATCTCACACATAAAACTCGTTCCCCTACTACTTTTCTTCCTTCTAAACTCAGTTTGTTCTACAACTTCAAACTCGATTTCGGAAAGCTTTGTTCAATGCTTTTCCTCCCGCATACATTCGAACTCAACAGGCAAAATCATCCTCACCGAAAGCTCTTCAGACTATACCCTAGTGTTGCAATCTTCCATACAGAATCTCCGATTCTTGAACACATCAACACCGAAACCACAAGCTATCATTACTCCTCATCAATACTCTCATGTTCAAGCAGCTGTGGTATGTGCCAAGAAAGTGGGCATACAAATAAGAACCCGAAGCGGAGGACATGACTATGAGGGCCTATCTTATGTGTCCAAATCTCCTTTCATCATCATTGATCTTTTCAATCTTCGATCCATTGATATTGACATAAAGAAGGAGAGGGCTTGGGTTGAGTCAGGTGCTACTCTTGGAGAGTTGTACTATGCAATTGCACAAAAGAGTAAACTCTATGGCTTTCCAGCAGGGACATGTCCAACTATTGGTGTGGGAGGACATATTAGTGGAGGTGGACATGGTACCTTGTGCAGACAATACGGTCTAGCTGCTGACAATGTCCTTGATGCTAAGATTGTCGACGTTAGCGGAAGAATTCTTGACAGAAAATCTATGGGAAAAGATCTCTTTTGGGCAATTCGAGGCGGCGGAGGCTCAAACTTTGGAGTCATCCTGGCCTGGAAACTTAGGTTGGTTCCTGTTCCTCCAATTGTAACAGGCTACATGGAATCGAAGACCATTGAACAAGGTGCAACCAAACTTGTTTCCAAATGGCAAGCTATAGCTGATAGGATGCCTCAACAGTACTTCCTGCGTTTGGTTATCCAAGTTGCAAATGGAACTGGTACAAATGGTGGAAAAACTATTCAAGTTACGTTCAATTTCGTGGCTTTTGAGACTGTTGAGAAACTTCTCCCTTGGATGCATGAAAATTTCCCTGAGTTAAATTTAGACCAGTCCACATTCACTGAAATGAGTTGGATTGAGTCCGCTCTATACGTTGCTAACATCCCGATAAACGAATCACAAGTTCTGCTTCAGCGGACTCAACAATCGAGGGTATTCTTCAAAAACAAATCAGATTTTGTTACTGAACCTATTTCAGAAGCTGCCTTAGAAGGTTTATGGAAAACAATGCTTGAATTGGATTCATTACAGTTGATACTGACACCTTACGGTGGAAGAATGAGTGAGATTTATGATTGGGAAATTCCTTTCCCACATAGAAAAGGAAATTTGTTTGAGATCCAGTATGTCGACAATTGGGTTGATGACGAAGAAACTGAGGAACGTATTAGCGGGGTGAGAAGTGTGTATGAGTACATGACACCATATGTTTCAAAGTCCCCAAGAGCCGCCTATTTGAATTATAGGGATCTTGATTTGGGGAGGAACAAGGATGCTAATACAAGCTATGCACAAGCAAGCGCTTGGGGATTGAAGTATTTCAAGAATAACTTTGGGAGACTTGTTCAAATAAAGACTTCAGTCGATCCTGATAATTTCTTTAGAGATGAACAAAGCCTCCCGGTGTTACAGTCTGCGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

532

Amino Acids

59.56

Weight (kDa)

7.62

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 78 - 214 1e-29 FAD binding domain
BBE PF08031 467 - 524 3.1e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 582
Acc65I GGTACC 1 cut(s) 563
AccB1I GGYRCC 1 cut(s) 563
AccB7I CCANNNNNTGG 1 cut(s) 527
AccBSI CCGCTC 1 cut(s) 1034
AccI GTMKAC 2 cut(s) 621, 1299
AciI CCGC 9 cut(s) 115, 324, 630, 687, 690, 1032, 1084, 1341, 1398
AclWI GGATC 4 cut(s) 393, 1282, 1425, 1529
AcsI RAATTY 5 cut(s) 636, 973, 988, 1251, 1276
AcuI CTGAAG 3 cut(s) 147, 1064, 1512
AfaI GTAC 5 cut(s) 467, 565, 848, 889, 1364
AfeI AGCGCT 1 cut(s) 1471
AfiI CCNNNNNNNGG 3 cut(s) 527, 859, 1220
AflIII ACRYGT 1 cut(s) 515
AjnI CCWGG 2 cut(s) 714, 719
AjuI GAANNNNNNNTTGG 4 cut(s) 353, 385, 716, 748
AloI GAACNNNNNNTCC 2 cut(s) 1551, 1583
AluBI AGCT 9 cut(s) 93, 159, 239, 278, 590, 821, 827, 1151, 1455
AluI AGCT 9 cut(s) 93, 159, 239, 278, 590, 821, 827, 1151, 1455
Alw26I GTCTC 2 cut(s) 932, 1501
AlwI GGATC 4 cut(s) 393, 1282, 1425, 1529
AlwNI CAGNNNCTG 1 cut(s) 1151
Aor51HI AGCGCT 1 cut(s) 1471
AoxI GGCC 2 cut(s) 343, 717
ApeKI GCWGC 3 cut(s) 275, 590, 1151
ApoI RAATTY 5 cut(s) 636, 973, 988, 1251, 1276
Asp700I GAANNNNTTC 1 cut(s) 1487
Asp718I GGTACC 1 cut(s) 563
AspLEI GCGC 1 cut(s) 1472
AspS9I GGNCC 1 cut(s) 343
AsuC2I CCSGG 1 cut(s) 1571
AsuHPI GGTGA 2 cut(s) 142, 1357
AsuII TTCGAA 1 cut(s) 125
BanI GGYRCC 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 782
BbvI GCAGC 3 cut(s) 287, 577, 1138
BciT130I CCWGG 2 cut(s) 716, 721
BcnI CCSGG 1 cut(s) 1571
BcoDI GTCTC 2 cut(s) 932, 1501
BfaI CTAG 2 cut(s) 176, 587
BfmI CTRYAG 1 cut(s) 822
BfoI RGCGCY 1 cut(s) 1473
BglII AGATCT 2 cut(s) 6, 664
BisI GCNGC 5 cut(s) 276, 591, 688, 1152, 1398
BlsI GCNGC 5 cut(s) 277, 592, 689, 1153, 1399
BmcAI AGTACT 1 cut(s) 848
Bme1390I CCNGG 3 cut(s) 716, 721, 1571
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 2 cut(s) 565, 738
BmrFI CCNGG 3 cut(s) 716, 721, 1571
BmsI GCATC 4 cut(s) 598, 825, 954, 1432
BoxI GACNNNNGTC 1 cut(s) 1529
BpiI GAAGAC 1 cut(s) 782
Bpu14I TTCGAA 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 1571
BsaJI CCNNGG 1 cut(s) 959
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 1551, 1581
Bsc4I CCNNNNNNNGG 3 cut(s) 527, 859, 1220
Bse1I ACTGG 3 cut(s) 889, 997, 1291
BseBI CCWGG 2 cut(s) 716, 721
BseDI CCNNGG 1 cut(s) 959
BseGI GGATG 6 cut(s) 144, 711, 840, 969, 1050, 1447
BseLI CCNNNNNNNGG 3 cut(s) 527, 859, 1220
BseMII CTCAG 3 cut(s) 69, 972, 1317
BseNI ACTGG 3 cut(s) 889, 997, 1291
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 287, 577, 1138
BsgI GTGCAG 1 cut(s) 592
BshFI GGCC 2 cut(s) 345, 719
BshNI GGYRCC 1 cut(s) 563
BsiSI CCGG 1 cut(s) 1571
BslFI GGGAC 2 cut(s) 526, 1372
BslI CCNNNNNNNGG 3 cut(s) 527, 859, 1220
BsmAI GTCTC 2 cut(s) 932, 1501
BsmFI GGGAC 2 cut(s) 526, 1372
BsnI GGCC 2 cut(s) 345, 719
Bsp119I TTCGAA 1 cut(s) 125
Bsp143I GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
BspACI CCGC 9 cut(s) 115, 324, 630, 687, 690, 1032, 1084, 1341, 1398
BspANI GGCC 2 cut(s) 345, 719
BspCNI CTCAG 3 cut(s) 68, 973, 1318
BspLI GGNNCC 2 cut(s) 565, 738
BspPI GGATC 4 cut(s) 393, 1282, 1425, 1529
BspQI GCTCTTC 1 cut(s) 166
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 563
BsrBI CCGCTC 1 cut(s) 1034
BsrI ACTGG 3 cut(s) 889, 997, 1291
BssECI CCNNGG 1 cut(s) 959
BssMI GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
BssT1I CCWWGG 1 cut(s) 959
Bst2UI CCWGG 2 cut(s) 716, 721
Bst4CI ACNGT 6 cut(s) 584, 846, 943, 1200, 1220, 1581
Bst6I CTCTTC 1 cut(s) 166
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 2 cut(s) 819, 1468
BstDEI CTNAG 6 cut(s) 55, 612, 728, 981, 1156, 1326
BstF5I GGATG 6 cut(s) 144, 711, 840, 969, 1050, 1447
BstH2I RGCGCY 1 cut(s) 1473
BstHHI GCGC 1 cut(s) 1472
BstKTI GATC 7 cut(s) 9, 385, 401, 667, 1290, 1420, 1537
BstMAI GTCTC 2 cut(s) 932, 1501
BstMBI GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
BstMWI GCNNNNNNNGC 1 cut(s) 693
BstNI CCWGG 2 cut(s) 716, 721
BstNSI RCATGY 1 cut(s) 519
BstPAI GACNNNNGTC 1 cut(s) 1529
BstSCI CCNGG 3 cut(s) 714, 719, 1569
BstSFI CTRYAG 1 cut(s) 822
BstV1I GCAGC 3 cut(s) 287, 577, 1138
BstV2I GAAGAC 1 cut(s) 782
BstX2I RGATCY 4 cut(s) 6, 664, 1287, 1417
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 4 cut(s) 6, 664, 1287, 1417
BsuRI GGCC 2 cut(s) 345, 719
BtsCI GGATG 6 cut(s) 144, 711, 840, 969, 1050, 1447
BtsIMutI CAGTG 1 cut(s) 1008
Cac8I GCNNGC 2 cut(s) 819, 1468
CaiI CAGNNNCTG 1 cut(s) 1151
CfoI GCGC 1 cut(s) 1472
Cfr13I GGNCC 1 cut(s) 343
Csp6I GTAC 5 cut(s) 466, 564, 847, 888, 1363
CviAII CATG 7 cut(s) 266, 332, 516, 560, 766, 968, 1366
CviQI GTAC 5 cut(s) 466, 564, 847, 888, 1363
DdeI CTNAG 6 cut(s) 55, 612, 728, 981, 1156, 1326
DpnI GATC 7 cut(s) 8, 384, 400, 666, 1289, 1419, 1536
DpnII GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
DrdI GACNNNNNNGTC 1 cut(s) 582
DseDI GACNNNNNNGTC 1 cut(s) 582
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
EciI GGCGGA 1 cut(s) 705
Eco130I CCWWGG 1 cut(s) 959
Eco47III AGCGCT 1 cut(s) 1471
Eco57I CTGAAG 3 cut(s) 147, 1064, 1512
EcoO109I RGGNCCY 1 cut(s) 343
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 714, 719
EcoT14I CCWWGG 1 cut(s) 959
EcoT22I ATGCAT 1 cut(s) 969
ErhI CCWWGG 1 cut(s) 959
FaeI CATG 7 cut(s) 269, 335, 519, 563, 769, 971, 1369
FalI AAGNNNNNCTT 4 cut(s) 1062, 1094, 1484, 1516
FaqI GGGAC 2 cut(s) 526, 1372
FatI CATG 7 cut(s) 265, 331, 515, 559, 765, 967, 1365
FauI CCCGC 2 cut(s) 122, 1334
FauNDI CATATG 1 cut(s) 1375
FblI GTMKAC 2 cut(s) 621, 1299
Fnu4HI GCNGC 5 cut(s) 276, 591, 688, 1152, 1398
FokI GGATG 6 cut(s) 131, 698, 847, 976, 1037, 1454
Fsp4HI GCNGC 5 cut(s) 276, 591, 688, 1152, 1398
FspBI CTAG 2 cut(s) 176, 587
GlaI GCGC 1 cut(s) 1471
GluI GCNGC 5 cut(s) 276, 591, 688, 1152, 1398
HaeII RGCGCY 1 cut(s) 1473
HaeIII GGCC 2 cut(s) 345, 719
HapII CCGG 1 cut(s) 1571
HhaI GCGC 1 cut(s) 1472
Hin1II CATG 7 cut(s) 269, 335, 519, 563, 769, 971, 1369
Hin6I GCGC 1 cut(s) 1470
HinP1I GCGC 1 cut(s) 1470
HincII GTYRAC 2 cut(s) 622, 1300
HindII GTYRAC 2 cut(s) 622, 1300
HindIII AAGCTT 1 cut(s) 91
HinfI GANTC 9 cut(s) 199, 207, 440, 708, 770, 1028, 1064, 1087, 1190
HpaII CCGG 1 cut(s) 1571
HphI GGTGA 2 cut(s) 142, 1357
Hpy166II GTNNAC 5 cut(s) 491, 557, 622, 1002, 1300
Hpy188I TCNGA 5 cut(s) 88, 166, 206, 1123, 1147
Hpy188III TCNNGA 6 cut(s) 211, 641, 1054, 1421, 1492, 1538
Hpy8I GTNNAC 5 cut(s) 491, 557, 622, 1002, 1300
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 3 cut(s) 56, 415, 1154
HpyCH4III ACNGT 6 cut(s) 584, 846, 943, 1200, 1220, 1581
HpyCH4IV ACGT 4 cut(s) 624, 917, 1041, 1333
HpyCH4V TGCA 8 cut(s) 184, 473, 479, 573, 794, 875, 967, 1460
HpyF10VI GCNNNNNNNGC 1 cut(s) 693
HpyF3I CTNAG 6 cut(s) 55, 612, 728, 981, 1156, 1326
HpySE526I ACGT 4 cut(s) 624, 917, 1041, 1333
Hsp92II CATG 7 cut(s) 269, 335, 519, 563, 769, 971, 1369
HspAI GCGC 1 cut(s) 1470
KpnI GGTACC 1 cut(s) 567
Kzo9I GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
LguI GCTCTTC 1 cut(s) 166
Lsp1109I GCAGC 3 cut(s) 287, 577, 1138
LweI GCATC 4 cut(s) 598, 825, 954, 1432
MaeI CTAG 2 cut(s) 176, 587
MaeII ACGT 4 cut(s) 624, 917, 1041, 1333
MaeIII GTNAC 4 cut(s) 754, 913, 1129, 1575
MalI GATC 7 cut(s) 8, 384, 400, 666, 1289, 1419, 1536
MbiI CCGCTC 1 cut(s) 1034
MboI GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
MboII GAAGA 9 cut(s) 34, 153, 180, 386, 645, 787, 1099, 1236, 1331
MfeI CAATTG 3 cut(s) 474, 750, 1303
MflI RGATCY 4 cut(s) 6, 664, 1287, 1417
MlyI GAGTC 4 cut(s) 449, 717, 1037, 1081
MmeI TCCRAC 2 cut(s) 545, 1001
Mph1103I ATGCAT 1 cut(s) 969
MroXI GAANNNNTTC 1 cut(s) 1487
MseI TTAA 1 cut(s) 986
MslI CAYNNNNRTG 3 cut(s) 336, 546, 1013
MspA1I CMGCKG 2 cut(s) 278, 1084
MspI CCGG 1 cut(s) 1571
MspR9I CCNGG 3 cut(s) 716, 721, 1571
MunI CAATTG 3 cut(s) 474, 750, 1303
MvaI CCWGG 2 cut(s) 716, 721
MwoI GCNNNNNNNGC 1 cut(s) 693
NciI CCSGG 1 cut(s) 1571
NdeI CATATG 1 cut(s) 1375
NdeII GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
NlaIII CATG 7 cut(s) 269, 335, 519, 563, 769, 971, 1369
NlaIV GGNNCC 2 cut(s) 565, 738
NsiI ATGCAT 1 cut(s) 969
NspI RCATGY 1 cut(s) 519
NspV TTCGAA 1 cut(s) 125
PciI ACATGT 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 1487
PfeI GAWTC 5 cut(s) 199, 207, 770, 1064, 1190
PflFI GACNNNGTC 1 cut(s) 599
PflMI CCANNNNNTGG 1 cut(s) 527
PkrI GCNGC 5 cut(s) 277, 592, 689, 1153, 1399
PleI GAGTC 4 cut(s) 448, 716, 1036, 1081
PpsI GAGTC 4 cut(s) 448, 716, 1036, 1081
PscI ACATGT 1 cut(s) 515
PshAI GACNNNNGTC 1 cut(s) 1529
Psp6I CCWGG 2 cut(s) 714, 719
PspGI CCWGG 2 cut(s) 714, 719
PspN4I GGNNCC 2 cut(s) 565, 738
PspPI GGNCC 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 4 cut(s) 6, 664, 1287, 1417
PsyI GACNNNGTC 1 cut(s) 599
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 5 cut(s) 467, 565, 848, 889, 1364
RsaNI GTAC 5 cut(s) 466, 564, 847, 888, 1363
RseI CAYNNNNRTG 3 cut(s) 336, 546, 1013
SalI GTCGAC 2 cut(s) 620, 1298
SapI GCTCTTC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 986
SatI GCNGC 5 cut(s) 276, 591, 688, 1152, 1398
Sau3AI GATC 7 cut(s) 6, 382, 398, 664, 1287, 1417, 1534
Sau96I GGNCC 1 cut(s) 343
ScaI AGTACT 1 cut(s) 848
SchI GAGTC 4 cut(s) 449, 717, 1037, 1081
ScrFI CCNGG 3 cut(s) 716, 721, 1571
SfaNI GCATC 4 cut(s) 598, 825, 954, 1432
SfcI CTRYAG 1 cut(s) 822
SfuI TTCGAA 1 cut(s) 125
SmiMI CAYNNNNRTG 3 cut(s) 336, 546, 1013
SsiI CCGC 9 cut(s) 115, 324, 630, 687, 690, 1032, 1084, 1341, 1398
SspMI CTAG 2 cut(s) 176, 587
StyD4I CCNGG 3 cut(s) 714, 719, 1569
StyI CCWWGG 1 cut(s) 959
TaaI ACNGT 6 cut(s) 584, 846, 943, 1200, 1220, 1581
TaiI ACGT 4 cut(s) 627, 920, 1044, 1336
TaqI TCGA 9 cut(s) 80, 125, 397, 621, 682, 773, 1097, 1299, 1533
TatI WGTACW 3 cut(s) 465, 846, 1362
TauI GCSGC 2 cut(s) 690, 1400
TfiI GAWTC 5 cut(s) 199, 207, 770, 1064, 1190
Tru1I TTAA 1 cut(s) 986
Tru9I TTAA 1 cut(s) 986
TscAI CASTG 1 cut(s) 1015
TseI GCWGC 3 cut(s) 275, 590, 1151
TspDTI ATGAA 4 cut(s) 361, 984, 1182, 1572
TspRI CASTG 1 cut(s) 1015
Tth111I GACNNNGTC 1 cut(s) 599
Van91I CCANNNNNTGG 1 cut(s) 527
XapI RAATTY 5 cut(s) 636, 973, 988, 1251, 1276
XceI RCATGY 1 cut(s) 519
XcmI CCANNNNNNNNNTGG 1 cut(s) 875
XmiI GTMKAC 2 cut(s) 621, 1299
XmnI GAANNNNTTC 1 cut(s) 1487
XspI CTAG 2 cut(s) 176, 587
ZrmI AGTACT 1 cut(s) 848
Zsp2I ATGCAT 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.