Belongs to the oxygen-dependent FAD-linked oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 101 |
| Acc65I | GGTACC | 1 cut(s) | 278 |
| AccB1I | GGYRCC | 1 cut(s) | 278 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2407 |
| AccI | GTMKAC | 1 cut(s) | 1518 |
| AccIII | TCCGGA | 1 cut(s) | 2092 |
| AciI | CCGC | 6 cut(s) | 395, 477, 1228, 1662, 2110, 2451 |
| AclWI | GGATC | 2 cut(s) | 2144, 2602 |
| AcsI | RAATTY | 6 cut(s) | 263, 467, 908, 1605, 2030, 2775 |
| AdeI | CACNNNGTG | 1 cut(s) | 2711 |
| AfaI | GTAC | 5 cut(s) | 12, 280, 932, 999, 1580 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 1198, 1437, 1438, 1640, 2330, 2407 |
| AflIII | ACRYGT | 2 cut(s) | 1628, 2443 |
| AjnI | CCWGG | 1 cut(s) | 2610 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 1466, 1498, 1829, 1861, 2175, 2207 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2624, 2656 |
| Alw26I | GTCTC | 2 cut(s) | 1032, 2569 |
| AlwI | GGATC | 2 cut(s) | 2144, 2602 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2118 |
| Ama87I | CYCGRG | 1 cut(s) | 370 |
| Aor13HI | TCCGGA | 1 cut(s) | 2092 |
| AoxI | GGCC | 4 cut(s) | 1441, 1456, 2051, 2107 |
| ApeKI | GCWGC | 3 cut(s) | 1388, 1703, 2112 |
| ApoI | RAATTY | 6 cut(s) | 263, 467, 908, 1605, 2030, 2775 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1681, 1713 |
| AseI | ATTAAT | 1 cut(s) | 2022 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 246, 2555 |
| Asp718I | GGTACC | 1 cut(s) | 278 |
| AspLEI | GCGC | 3 cut(s) | 971, 1907, 2003 |
| AspS9I | GGNCC | 3 cut(s) | 1441, 1456, 2051 |
| AsuHPI | GGTGA | 1 cut(s) | 1255 |
| AsuII | TTCGAA | 2 cut(s) | 1187, 1922 |
| AvaI | CYCGRG | 1 cut(s) | 370 |
| BanI | GGYRCC | 1 cut(s) | 278 |
| BbsI | GAAGAC | 2 cut(s) | 954, 1962 |
| BbvI | GCAGC | 3 cut(s) | 1400, 1715, 2124 |
| BccI | CCATC | 7 cut(s) | 484, 752, 1108, 1870, 2417, 2661, 2815 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1735, 1769, 2374, 2408 |
| BciT130I | CCWGG | 1 cut(s) | 2612 |
| BciVI | GTATCC | 1 cut(s) | 1480 |
| BcoDI | GTCTC | 2 cut(s) | 1032, 2569 |
| BfaI | CTAG | 4 cut(s) | 629, 1700, 2489, 2811 |
| BfuI | GTATCC | 1 cut(s) | 1480 |
| BglII | AGATCT | 1 cut(s) | 1119 |
| BisI | GCNGC | 4 cut(s) | 1389, 1704, 2110, 2113 |
| BlsI | GCNGC | 4 cut(s) | 1390, 1705, 2111, 2114 |
| BmcAI | AGTACT | 1 cut(s) | 932 |
| Bme1390I | CCNGG | 1 cut(s) | 2612 |
| BmeT110I | CYCGRG | 1 cut(s) | 370 |
| BmgT120I | GGNCC | 3 cut(s) | 1441, 1456, 2051 |
| BmiI | GGNNCC | 6 cut(s) | 280, 1442, 1479, 1851, 2038, 2481 |
| BmrFI | CCNGG | 1 cut(s) | 2612 |
| BmsI | GCATC | 2 cut(s) | 2067, 2648 |
| BpiI | GAAGAC | 2 cut(s) | 954, 1962 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1764, 1796 |
| Bpu14I | TTCGAA | 2 cut(s) | 1187, 1922 |
| BpuEI | CTTGAG | 1 cut(s) | 1795 |
| Bsa29I | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1118, 2682 |
| BsaJI | CCNNGG | 5 cut(s) | 371, 786, 978, 1473, 1767 |
| BsaWI | WCCGGW | 1 cut(s) | 2092 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1299, 1329 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 1198, 1437, 1438, 1640, 2330, 2407 |
| Bse118I | RCCGGY | 1 cut(s) | 2159 |
| Bse1I | ACTGG | 3 cut(s) | 806, 2054, 2401 |
| Bse3DI | GCAATG | 1 cut(s) | 1935 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1118, 2682 |
| BseAI | TCCGGA | 1 cut(s) | 2092 |
| BseBI | CCWGG | 1 cut(s) | 2612 |
| BseCI | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| BseDI | CCNNGG | 5 cut(s) | 371, 786, 978, 1473, 1767 |
| BseGI | GGATG | 8 cut(s) | 231, 745, 849, 1223, 1257, 2425, 2460, 2639 |
| BseJI | GATNNNNATC | 2 cut(s) | 1118, 2682 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 1198, 1437, 1438, 1640, 2330, 2407 |
| BseMI | GCAATG | 1 cut(s) | 1935 |
| BseMII | CTCAG | 3 cut(s) | 1182, 1302, 2427 |
| BseNI | ACTGG | 3 cut(s) | 806, 2054, 2401 |
| BseRI | GAGGAG | 2 cut(s) | 765, 1812 |
| BseXI | GCAGC | 3 cut(s) | 1400, 1715, 2124 |
| BsgI | GTGCAG | 1 cut(s) | 2104 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2606 |
| BshFI | GGCC | 4 cut(s) | 1443, 1458, 2053, 2109 |
| BshNI | GGYRCC | 1 cut(s) | 278 |
| BshVI | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| BsiEI | CGRYCG | 1 cut(s) | 2606 |
| BsiHKCI | CYCGRG | 1 cut(s) | 370 |
| BsiSI | CCGG | 2 cut(s) | 2093, 2160 |
| BslFI | GGGAC | 4 cut(s) | 1196, 1639, 1665, 2745 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 1198, 1437, 1438, 1640, 2330, 2407 |
| BsmAI | GTCTC | 2 cut(s) | 1032, 2569 |
| BsmFI | GGGAC | 4 cut(s) | 1196, 1639, 1665, 2745 |
| BsnI | GGCC | 4 cut(s) | 1443, 1458, 2053, 2109 |
| BsoBI | CYCGRG | 1 cut(s) | 370 |
| Bsp119I | TTCGAA | 2 cut(s) | 1187, 1922 |
| Bsp13I | TCCGGA | 1 cut(s) | 2092 |
| Bsp1407I | TGTACA | 1 cut(s) | 997 |
| Bsp143I | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| Bsp19I | CCATGG | 2 cut(s) | 786, 1767 |
| BspACI | CCGC | 6 cut(s) | 395, 477, 1228, 1662, 2110, 2451 |
| BspANI | GGCC | 4 cut(s) | 1443, 1458, 2053, 2109 |
| BspCNI | CTCAG | 3 cut(s) | 1181, 1301, 2428 |
| BspDI | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| BspEI | TCCGGA | 1 cut(s) | 2092 |
| BspHI | TCATGA | 2 cut(s) | 31, 1951 |
| BspLI | GGNNCC | 6 cut(s) | 280, 1442, 1479, 1851, 2038, 2481 |
| BspPI | GGATC | 2 cut(s) | 2144, 2602 |
| BspT104I | TTCGAA | 2 cut(s) | 1187, 1922 |
| BspT107I | GGYRCC | 1 cut(s) | 278 |
| BsrDI | GCAATG | 1 cut(s) | 1935 |
| BsrFI | RCCGGY | 1 cut(s) | 2159 |
| BsrGI | TGTACA | 1 cut(s) | 997 |
| BsrI | ACTGG | 3 cut(s) | 806, 2054, 2401 |
| BssAI | RCCGGY | 1 cut(s) | 2159 |
| BssECI | CCNNGG | 5 cut(s) | 371, 786, 978, 1473, 1767 |
| BssMI | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| BssT1I | CCWWGG | 4 cut(s) | 786, 978, 1473, 1767 |
| Bst2UI | CCWGG | 1 cut(s) | 2612 |
| Bst4CI | ACNGT | 7 cut(s) | 38, 188, 452, 602, 616, 850, 1873 |
| Bst6I | CTCTTC | 2 cut(s) | 718, 724 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2118 |
| BstAUI | TGTACA | 1 cut(s) | 997 |
| BstBI | TTCGAA | 2 cut(s) | 1187, 1922 |
| BstC8I | GCNNGC | 4 cut(s) | 1999, 2161, 2266, 2536 |
| BstDEI | CTNAG | 8 cut(s) | 952, 1168, 1288, 1725, 1841, 2211, 2436, 2596 |
| BstDSI | CCRYGG | 2 cut(s) | 786, 1767 |
| BstF5I | GGATG | 8 cut(s) | 231, 745, 849, 1223, 1257, 2425, 2460, 2639 |
| BstHHI | GCGC | 3 cut(s) | 971, 1907, 2003 |
| BstKTI | GATC | 9 cut(s) | 257, 364, 634, 638, 1122, 1498, 2139, 2606, 2610 |
| BstMAI | GTCTC | 2 cut(s) | 1032, 2569 |
| BstMBI | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| BstMCI | CGRYCG | 1 cut(s) | 2606 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1394, 2118, 2544 |
| BstNI | CCWGG | 1 cut(s) | 2612 |
| BstNSI | RCATGY | 5 cut(s) | 426, 1401, 1632, 2167, 2447 |
| BstSCI | CCNGG | 1 cut(s) | 2610 |
| BstV1I | GCAGC | 3 cut(s) | 1400, 1715, 2124 |
| BstV2I | GAAGAC | 2 cut(s) | 954, 1962 |
| BstX2I | RGATCY | 1 cut(s) | 1119 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 70 |
| BstYI | RGATCY | 1 cut(s) | 1119 |
| Bsu15I | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| BsuI | GTATCC | 1 cut(s) | 1480 |
| BsuRI | GGCC | 4 cut(s) | 1443, 1458, 2053, 2109 |
| BsuTUI | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| BtgI | CCRYGG | 2 cut(s) | 786, 1767 |
| BtsCI | GGATG | 8 cut(s) | 231, 745, 849, 1223, 1257, 2425, 2460, 2639 |
| BtsIMutI | CAGTG | 3 cut(s) | 88, 2121, 2288 |
| Cac8I | GCNNGC | 4 cut(s) | 1999, 2161, 2266, 2536 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2118 |
| CciI | TCATGA | 2 cut(s) | 31, 1951 |
| CfoI | GCGC | 3 cut(s) | 971, 1907, 2003 |
| Cfr10I | RCCGGY | 1 cut(s) | 2159 |
| Cfr13I | GGNCC | 3 cut(s) | 1441, 1456, 2051 |
| ClaI | ATCGAT | 3 cut(s) | 634, 704, 2606 |
| CseI | GACGC | 1 cut(s) | 1730 |
| Csp6I | GTAC | 5 cut(s) | 11, 279, 931, 998, 1579 |
| CviQI | GTAC | 5 cut(s) | 11, 279, 931, 998, 1579 |
| DdeI | CTNAG | 8 cut(s) | 952, 1168, 1288, 1725, 1841, 2211, 2436, 2596 |
| DpnI | GATC | 9 cut(s) | 256, 363, 633, 637, 1121, 1497, 2138, 2605, 2609 |
| DpnII | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| DraIII | CACNNNGTG | 1 cut(s) | 2711 |
| Eam1104I | CTCTTC | 2 cut(s) | 718, 724 |
| EarI | CTCTTC | 2 cut(s) | 718, 724 |
| Eco130I | CCWWGG | 4 cut(s) | 786, 978, 1473, 1767 |
| Eco88I | CYCGRG | 1 cut(s) | 370 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1456 |
| EcoRI | GAATTC | 1 cut(s) | 2030 |
| EcoRII | CCWGG | 1 cut(s) | 2610 |
| EcoT14I | CCWWGG | 4 cut(s) | 786, 978, 1473, 1767 |
| EcoT22I | ATGCAT | 2 cut(s) | 326, 528 |
| ErhI | CCWWGG | 4 cut(s) | 786, 978, 1473, 1767 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1466, 1498, 2175, 2207, 2204, 2236 |
| FaqI | GGGAC | 4 cut(s) | 1196, 1639, 1665, 2745 |
| FauI | CCCGC | 2 cut(s) | 1235, 2444 |
| FauNDI | CATATG | 2 cut(s) | 57, 1096 |
| FblI | GTMKAC | 1 cut(s) | 1518 |
| Fnu4HI | GCNGC | 4 cut(s) | 1389, 1704, 2110, 2113 |
| FokI | GGATG | 8 cut(s) | 218, 752, 856, 1210, 1244, 2432, 2467, 2626 |
| Fsp4HI | GCNGC | 4 cut(s) | 1389, 1704, 2110, 2113 |
| FspBI | CTAG | 4 cut(s) | 629, 1700, 2489, 2811 |
| GlaI | GCGC | 3 cut(s) | 970, 1906, 2002 |
| GluI | GCNGC | 4 cut(s) | 1389, 1704, 2110, 2113 |
| HaeIII | GGCC | 4 cut(s) | 1443, 1458, 2053, 2109 |
| HapII | CCGG | 2 cut(s) | 2093, 2160 |
| HgaI | GACGC | 1 cut(s) | 1730 |
| HhaI | GCGC | 3 cut(s) | 971, 1907, 2003 |
| Hin6I | GCGC | 3 cut(s) | 969, 1905, 2001 |
| HinP1I | GCGC | 3 cut(s) | 969, 1905, 2001 |
| HincII | GTYRAC | 5 cut(s) | 384, 942, 1519, 1525, 1735 |
| HindII | GTYRAC | 5 cut(s) | 384, 942, 1519, 1525, 1735 |
| HindIII | AAGCTT | 4 cut(s) | 1204, 1813, 2184, 2304 |
| HpaI | GTTAAC | 1 cut(s) | 942 |
| HpaII | CCGG | 2 cut(s) | 2093, 2160 |
| HphI | GGTGA | 1 cut(s) | 1255 |
| Hpy166II | GTNNAC | 5 cut(s) | 384, 942, 1519, 1525, 1735 |
| Hpy8I | GTNNAC | 5 cut(s) | 384, 942, 1519, 1525, 1735 |
| Hpy99I | CGWCG | 1 cut(s) | 1523 |
| HpyAV | CCTTC | 6 cut(s) | 766, 1169, 1374, 1402, 1492, 2454 |
| HpyCH4III | ACNGT | 7 cut(s) | 38, 188, 452, 602, 616, 850, 1873 |
| HpyCH4IV | ACGT | 3 cut(s) | 572, 1521, 1737 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1394, 2118, 2544 |
| HpyF3I | CTNAG | 8 cut(s) | 952, 1168, 1288, 1725, 1841, 2211, 2436, 2596 |
| HpySE526I | ACGT | 3 cut(s) | 572, 1521, 1737 |
| HspAI | GCGC | 3 cut(s) | 969, 1905, 2001 |
| Kpn2I | TCCGGA | 1 cut(s) | 2092 |
| KpnI | GGTACC | 1 cut(s) | 282 |
| KroI | GCCGGC | 1 cut(s) | 2159 |
| KroNI | GCCGGC | 1 cut(s) | 2161 |
| KspAI | GTTAAC | 1 cut(s) | 942 |
| Kzo9I | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| LmnI | GCTCC | 2 cut(s) | 788, 1483 |
| Lsp1109I | GCAGC | 3 cut(s) | 1400, 1715, 2124 |
| LweI | GCATC | 2 cut(s) | 2067, 2648 |
| MaeI | CTAG | 4 cut(s) | 629, 1700, 2489, 2811 |
| MaeII | ACGT | 3 cut(s) | 572, 1521, 1737 |
| MaeIII | GTNAC | 8 cut(s) | 73, 988, 1076, 1867, 2242, 2410, 2614, 2703 |
| MalI | GATC | 9 cut(s) | 256, 363, 633, 637, 1121, 1497, 2138, 2605, 2609 |
| MboI | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| MfeI | CAATTG | 1 cut(s) | 1587 |
| MflI | RGATCY | 1 cut(s) | 1119 |
| MlyI | GAGTC | 5 cut(s) | 320, 1073, 1562, 2150, 2186 |
| MmeI | TCCRAC | 5 cut(s) | 821, 1079, 1658, 1966, 2160 |
| Mph1103I | ATGCAT | 2 cut(s) | 326, 528 |
| MroI | TCCGGA | 1 cut(s) | 2092 |
| MroNI | GCCGGC | 1 cut(s) | 2159 |
| MroXI | GAANNNNTTC | 2 cut(s) | 246, 2555 |
| MslI | CAYNNNNRTG | 7 cut(s) | 319, 521, 791, 1539, 2075, 2126, 2644 |
| MspA1I | CMGCKG | 2 cut(s) | 1391, 1706 |
| MspI | CCGG | 2 cut(s) | 2093, 2160 |
| MspR9I | CCNGG | 1 cut(s) | 2612 |
| MunI | CAATTG | 1 cut(s) | 1587 |
| MvaI | CCWGG | 1 cut(s) | 2612 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1394, 2118, 2544 |
| NaeI | GCCGGC | 1 cut(s) | 2161 |
| NcoI | CCATGG | 2 cut(s) | 786, 1767 |
| NdeI | CATATG | 2 cut(s) | 57, 1096 |
| NdeII | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| NgoMIV | GCCGGC | 1 cut(s) | 2159 |
| NlaIV | GGNNCC | 6 cut(s) | 280, 1442, 1479, 1851, 2038, 2481 |
| NmuCI | GTSAC | 6 cut(s) | 988, 1076, 1867, 2242, 2410, 2703 |
| NsiI | ATGCAT | 2 cut(s) | 326, 528 |
| NspI | RCATGY | 5 cut(s) | 426, 1401, 1632, 2167, 2447 |
| NspV | TTCGAA | 2 cut(s) | 1187, 1922 |
| PagI | TCATGA | 2 cut(s) | 31, 1951 |
| PciI | ACATGT | 2 cut(s) | 1628, 2443 |
| PdiI | GCCGGC | 1 cut(s) | 2161 |
| PdmI | GAANNNNTTC | 2 cut(s) | 246, 2555 |
| PfeI | GAWTC | 7 cut(s) | 898, 1312, 1320, 1750, 2354, 2379, 2397 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2407 |
| PkrI | GCNGC | 4 cut(s) | 1390, 1705, 2111, 2114 |
| Ple19I | CGATCG | 1 cut(s) | 2606 |
| PleI | GAGTC | 5 cut(s) | 319, 1073, 1561, 2149, 2185 |
| PpsI | GAGTC | 5 cut(s) | 319, 1073, 1561, 2149, 2185 |
| PscI | ACATGT | 2 cut(s) | 1628, 2443 |
| PshBI | ATTAAT | 1 cut(s) | 2022 |
| PsiI | TTATAA | 1 cut(s) | 101 |
| Psp6I | CCWGG | 1 cut(s) | 2610 |
| PspGI | CCWGG | 1 cut(s) | 2610 |
| PspN4I | GGNNCC | 6 cut(s) | 280, 1442, 1479, 1851, 2038, 2481 |
| PspPI | GGNCC | 3 cut(s) | 1441, 1456, 2051 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2118 |
| PsuI | RGATCY | 1 cut(s) | 1119 |
| PvuI | CGATCG | 1 cut(s) | 2606 |
| PvuII | CAGCTG | 2 cut(s) | 1391, 1706 |
| RsaI | GTAC | 5 cut(s) | 12, 280, 932, 999, 1580 |
| RsaNI | GTAC | 5 cut(s) | 11, 279, 931, 998, 1579 |
| RseI | CAYNNNNRTG | 7 cut(s) | 319, 521, 791, 1539, 2075, 2126, 2644 |
| SalI | GTCGAC | 1 cut(s) | 1517 |
| SatI | GCNGC | 4 cut(s) | 1389, 1704, 2110, 2113 |
| Sau3AI | GATC | 9 cut(s) | 254, 361, 631, 635, 1119, 1495, 2136, 2603, 2607 |
| Sau96I | GGNCC | 3 cut(s) | 1441, 1456, 2051 |
| ScaI | AGTACT | 1 cut(s) | 932 |
| SchI | GAGTC | 5 cut(s) | 320, 1073, 1562, 2150, 2186 |
| ScrFI | CCNGG | 1 cut(s) | 2612 |
| SfaNI | GCATC | 2 cut(s) | 2067, 2648 |
| SfuI | TTCGAA | 2 cut(s) | 1187, 1922 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 319, 521, 791, 1539, 2075, 2126, 2644 |
| SmlI | CTYRAG | 1 cut(s) | 1810 |
| SmoI | CTYRAG | 1 cut(s) | 1810 |
| SsiI | CCGC | 6 cut(s) | 395, 477, 1228, 1662, 2110, 2451 |
| SspI | AATATT | 6 cut(s) | 211, 247, 501, 625, 1005, 2026 |
| SspMI | CTAG | 4 cut(s) | 629, 1700, 2489, 2811 |
| StyD4I | CCNGG | 1 cut(s) | 2610 |
| StyI | CCWWGG | 4 cut(s) | 786, 978, 1473, 1767 |
| TaaI | ACNGT | 7 cut(s) | 38, 188, 452, 602, 616, 850, 1873 |
| TaiI | ACGT | 3 cut(s) | 575, 1524, 1740 |
| TaqII | GACCGA | 1 cut(s) | 1500 |
| TatI | WGTACW | 4 cut(s) | 10, 930, 997, 1578 |
| TauI | GCSGC | 1 cut(s) | 2112 |
| TfiI | GAWTC | 7 cut(s) | 898, 1312, 1320, 1750, 2354, 2379, 2397 |
| TscAI | CASTG | 3 cut(s) | 88, 2128, 2295 |
| TseFI | GTSAC | 6 cut(s) | 988, 1076, 1867, 2242, 2410, 2703 |
| TseI | GCWGC | 3 cut(s) | 1388, 1703, 2112 |
| Tsp45I | GTSAC | 6 cut(s) | 988, 1076, 1867, 2242, 2410, 2703 |
| TspGWI | ACGGA | 2 cut(s) | 762, 812 |
| TspRI | CASTG | 3 cut(s) | 88, 2128, 2295 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2407 |
| VspI | ATTAAT | 1 cut(s) | 2022 |
| XapI | RAATTY | 6 cut(s) | 263, 467, 908, 1605, 2030, 2775 |
| XbaI | TCTAGA | 2 cut(s) | 628, 2488 |
| XceI | RCATGY | 5 cut(s) | 426, 1401, 1632, 2167, 2447 |
| XmiI | GTMKAC | 1 cut(s) | 1518 |
| XmnI | GAANNNNTTC | 2 cut(s) | 246, 2555 |
| XspI | CTAG | 4 cut(s) | 629, 1700, 2489, 2811 |
| ZrmI | AGTACT | 1 cut(s) | 932 |
| Zsp2I | ATGCAT | 2 cut(s) | 326, 528 |