MD10G1244300.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000925
Physical Location & Seq
Forward (+)
33898149 .. 33908051
9903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1244300.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGAGATCTCCCACATAAAACTACTTCCCCTACTACTTTTCTTCCTTCTAAATTCAGTTTGTTCTACAACTTCGAACCCGATTTCGGAAAGCTTTGTCCCGTGCTTTTCATCCCGCATACATTCTAACTCAACAAGCAAAATCATCCTCACCGAAAGCACCTCGGATTATTCCTTAGTCTTGCAATCCTCCATACAGAATCTCCGATTCTTGAACACATCAACACCGAAACCACATGCTATTATTACTCCTTCTCAATACTCTCATGTTCAGGCAGCTGTGGTATGCGCCAAGAAAGACGGCATACAAATAAGAACCCGAAGCGGGGGCCATGACTACGAGGGCCTATCTTATGTATCCGAAGCTCCTTTCATCATCATTGATCTTTTCAATCTTCGGTCTGTCGATGTTGACATAGAGAATGAGAGTGCTTGGGTTGAGTCAGGTGCTACTCTCGGAGAGTTGTACTACACAATCGCACAAAAGAGTAAAATTCATGGATTTCCAGCAGGGTCATGTCCAACTATGGGTGTGGGGGGACATATTAGCGGAGGTGGATTTGGCACTATATTCCGAAAATATGGTCTAGCAGCTGACAATGTTATTGACGCTAAGATTGTTGACGTTAATGGCAGGATTCTGGACAGAAAATCCATGGGAGAAGAACTCTTTTGGGCTATTCGAGGTGGGGGAGGCTCAAGCTTTGCAGTCATTTTGAAATGGAAACTTAGGTTGGTTCCTGTTCCTCCATCTGTGACAGTTTTCAAACTTTCACAGACCATAGAACAAGGCGCAACCAAACTATTTTCGAAATGGCAAAACATTGCAGATAAACTTCATGAAGACATTTTCCTACATTCAGTCGTAAGTGTTGGAAATAAAGCTGGCGCAAATGGTGGCAAAACTATTATAATTGAATTTGGTTCCTTGTTTCTTGGGCCAAAACTTCTCCCTCTGATGCAAGACAATTTTCCGGAGTTACGTTTAGACCGGAGCAACTGCACTGAAATGAGCTGGATTGAGTCTGTTCTATACTTCGCCTACATCTCAATAAACGAGTCGGAAGCTTTGCTCAGGAGAACTCAACAATCTAAGAGCTTCTTCAAAGCAAAATCAGACTATGTGACTGAACCAATTTCGGAAGCTGGCTTGGAAGGTTTATGGCAAACACTGATTGAAGCAGAAGCTTATTTGATATTGACACCTTATGGTGGAAAAATGAGTGAGATTTCAGATTCGGAAACTCCTTTCCCACACAGAATTGGCAACATATTCAAAATCCAGTATATGGTCACTTGGGATGATGGCAAGGAAACTGAGAAATATATTGGCATGATGAGAAGGCTGTATGATTACATGGCACCATATGTTTCAAAGTCCCCAAGAGGTGCCTATTTGAATTATAGGGATCTTGACTTGGGGAGGAACAATGCTTGTAATACAAGCTATGGACAAGCAAGCATTTGGGGCTTAAAGTATTTCAAGAATAATTTTAGGAGACTTGTTCATGTGAAGACCTTAGTTGATCCTGCTAACTTCTTTAGGAATGAACAAAGCATCCCTGTGTTCCCATCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

527

Amino Acids

58.73

Weight (kDa)

8.32

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 78 - 214 2.5e-26 FAD binding domain
BBE PF08031 464 - 521 1e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 911
AccB1I GGYRCC 2 cut(s) 1360, 1388
AccB7I CCANNNNNTGG 1 cut(s) 1288
AccIII TCCGGA 1 cut(s) 973
AciI CCGC 3 cut(s) 115, 324, 549
AclWI GGATC 2 cut(s) 1416, 1520
AcsI RAATTY 3 cut(s) 52, 492, 917
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 6 cut(s) 85, 324, 325, 527, 1211, 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AloI GAACNNNNNNTCC 2 cut(s) 1542, 1574
Alw26I GTCTC 1 cut(s) 1492
AlwI GGATC 2 cut(s) 1416, 1520
Aor13HI TCCGGA 1 cut(s) 973
AoxI GGCC 3 cut(s) 328, 343, 938
ApeKI GCWGC 2 cut(s) 275, 590
ApoI RAATTY 3 cut(s) 52, 492, 917
ArsI GACNNNNNNTTYG 2 cut(s) 568, 600
AspLEI GCGC 3 cut(s) 290, 794, 890
AspS9I GGNCC 3 cut(s) 328, 343, 938
AsuHPI GGTGA 1 cut(s) 142
AsuII TTCGAA 2 cut(s) 74, 809
BanI GGYRCC 2 cut(s) 1360, 1388
BbsI GAAGAC 2 cut(s) 849, 1520
BbvI GCAGC 2 cut(s) 287, 602
BccI CCATC 3 cut(s) 757, 1298, 1579
BceAI ACGGC 1 cut(s) 316
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 1492
BfaI CTAG 2 cut(s) 587, 1575
BfuI GTATCC 1 cut(s) 367
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 276, 591
BlsI GCNGC 2 cut(s) 277, 592
BmgT120I GGNCC 3 cut(s) 328, 343, 938
BmiI GGNNCC 5 cut(s) 329, 738, 925, 1362, 1390
BmsI GCATC 2 cut(s) 948, 1566
BpiI GAAGAC 2 cut(s) 849, 1520
BplI GAGNNNNNCTC 2 cut(s) 651, 683
Bpu10I CCTNAGC 1 cut(s) 1073
Bpu14I TTCGAA 2 cut(s) 74, 809
BpuEI CTTGAG 1 cut(s) 682
BsaJI CCNNGG 2 cut(s) 162, 654
BsaWI WCCGGW 2 cut(s) 973, 990
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 324, 325, 527, 1211, 1288
Bse1I ACTGG 1 cut(s) 1282
Bse3DI GCAATG 1 cut(s) 822
BseAI TCCGGA 1 cut(s) 973
BseDI CCNNGG 2 cut(s) 162, 654
BseGI GGATG 4 cut(s) 110, 144, 1306, 1557
BseLI CCNNNNNNNGG 6 cut(s) 85, 324, 325, 527, 1211, 1288
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 2 cut(s) 1087, 1308
BseNI ACTGG 1 cut(s) 1282
BseXI GCAGC 2 cut(s) 287, 602
BsgI GTGCAG 1 cut(s) 985
BshFI GGCC 3 cut(s) 330, 345, 940
BshNI GGYRCC 2 cut(s) 1360, 1388
BsiSI CCGG 2 cut(s) 974, 991
BslFI GGGAC 3 cut(s) 83, 552, 1363
BslI CCNNNNNNNGG 6 cut(s) 85, 324, 325, 527, 1211, 1288
BsmAI GTCTC 1 cut(s) 1492
BsmFI GGGAC 3 cut(s) 83, 552, 1363
BsnI GGCC 3 cut(s) 330, 345, 940
Bsp119I TTCGAA 2 cut(s) 74, 809
Bsp13I TCCGGA 1 cut(s) 973
Bsp143I GATC 4 cut(s) 6, 382, 1408, 1525
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 3 cut(s) 115, 324, 549
BspANI GGCC 3 cut(s) 330, 345, 940
BspCNI CTCAG 2 cut(s) 1086, 1309
BspEI TCCGGA 1 cut(s) 973
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 5 cut(s) 329, 738, 925, 1362, 1390
BspPI GGATC 2 cut(s) 1416, 1520
BspT104I TTCGAA 2 cut(s) 74, 809
BspT107I GGYRCC 2 cut(s) 1360, 1388
BsrDI GCAATG 1 cut(s) 822
BsrI ACTGG 1 cut(s) 1282
BssECI CCNNGG 2 cut(s) 162, 654
BssMI GATC 4 cut(s) 6, 382, 1408, 1525
BssT1I CCWWGG 1 cut(s) 654
Bst4CI ACNGT 1 cut(s) 760
BstBI TTCGAA 2 cut(s) 74, 809
BstC8I GCNNGC 3 cut(s) 886, 1147, 1459
BstDEI CTNAG 7 cut(s) 175, 612, 728, 1073, 1092, 1317, 1519
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 4 cut(s) 110, 144, 1306, 1557
BstHHI GCGC 3 cut(s) 290, 794, 890
BstKTI GATC 4 cut(s) 9, 385, 1411, 1528
BstMAI GTCTC 1 cut(s) 1492
BstMBI GATC 4 cut(s) 6, 382, 1408, 1525
BstMWI GCNNNNNNNGC 1 cut(s) 1467
BstNSI RCATGY 1 cut(s) 239
BstV1I GCAGC 2 cut(s) 287, 602
BstV2I GAAGAC 2 cut(s) 849, 1520
BstX2I RGATCY 2 cut(s) 6, 1408
BstYI RGATCY 2 cut(s) 6, 1408
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 330, 345, 940
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 4 cut(s) 110, 144, 1306, 1557
BtsIMutI CAGTG 2 cut(s) 1002, 1169
Cac8I GCNNGC 3 cut(s) 886, 1147, 1459
CciI TCATGA 1 cut(s) 838
CfoI GCGC 3 cut(s) 290, 794, 890
Cfr13I GGNCC 3 cut(s) 328, 343, 938
CseI GACGC 1 cut(s) 617
Csp6I GTAC 1 cut(s) 466
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 7 cut(s) 175, 612, 728, 1073, 1092, 1317, 1519
DpnI GATC 4 cut(s) 8, 384, 1410, 1527
DpnII GATC 4 cut(s) 6, 382, 1408, 1525
Eco130I CCWWGG 1 cut(s) 654
EcoO109I RGGNCCY 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FalI AAGNNNNNCTT 2 cut(s) 1085, 1117
FaqI GGGAC 3 cut(s) 83, 552, 1363
FauI CCCGC 2 cut(s) 122, 317
FauNDI CATATG 1 cut(s) 1366
Fnu4HI GCNGC 2 cut(s) 276, 591
FokI GGATG 4 cut(s) 97, 131, 1313, 1544
Fsp4HI GCNGC 2 cut(s) 276, 591
FspBI CTAG 2 cut(s) 587, 1575
GlaI GCGC 3 cut(s) 289, 793, 889
GluI GCNGC 2 cut(s) 276, 591
HaeIII GGCC 3 cut(s) 330, 345, 940
HapII CCGG 2 cut(s) 974, 991
HgaI GACGC 1 cut(s) 617
HhaI GCGC 3 cut(s) 290, 794, 890
Hin6I GCGC 3 cut(s) 288, 792, 888
HinP1I GCGC 3 cut(s) 288, 792, 888
HincII GTYRAC 2 cut(s) 412, 622
HindII GTYRAC 2 cut(s) 412, 622
HindIII AAGCTT 4 cut(s) 91, 700, 1065, 1185
HinfI GANTC 7 cut(s) 199, 207, 440, 637, 1022, 1058, 1235
HpaII CCGG 2 cut(s) 974, 991
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 412, 622
Hpy188III TCNNGA 8 cut(s) 211, 641, 839, 974, 1075, 1412, 1483, 1575
Hpy8I GTNNAC 2 cut(s) 412, 622
HpyAV CCTTC 4 cut(s) 56, 261, 1148, 1335
HpyCH4III ACNGT 1 cut(s) 760
HpyCH4IV ACGT 2 cut(s) 624, 982
HpyCH4V TGCA 5 cut(s) 184, 707, 827, 961, 1002
HpyF10VI GCNNNNNNNGC 1 cut(s) 1467
HpyF3I CTNAG 7 cut(s) 175, 612, 728, 1073, 1092, 1317, 1519
HpySE526I ACGT 2 cut(s) 624, 982
HspAI GCGC 3 cut(s) 288, 792, 888
Kpn2I TCCGGA 1 cut(s) 973
Kzo9I GATC 4 cut(s) 6, 382, 1408, 1525
LmnI GCTCC 2 cut(s) 370, 993
Lsp1109I GCAGC 2 cut(s) 287, 602
LweI GCATC 2 cut(s) 948, 1566
MaeI CTAG 2 cut(s) 587, 1575
MaeII ACGT 2 cut(s) 624, 982
MaeIII GTNAC 4 cut(s) 754, 978, 1123, 1291
MalI GATC 4 cut(s) 8, 384, 1410, 1527
MboI GATC 4 cut(s) 6, 382, 1408, 1525
MboII GAAGA 6 cut(s) 34, 386, 674, 854, 1093, 1525
MflI RGATCY 2 cut(s) 6, 1408
MluCI AATT 9 cut(s) 52, 492, 912, 917, 967, 1134, 1260, 1399, 1489
MlyI GAGTC 3 cut(s) 449, 1031, 1067
MmeI TCCRAC 3 cut(s) 545, 853, 1041
MroI TCCGGA 1 cut(s) 973
MseI TTAA 2 cut(s) 627, 1472
MslI CAYNNNNRTG 3 cut(s) 1007, 1562, 1576
MspA1I CMGCKG 2 cut(s) 278, 593
MspI CCGG 2 cut(s) 974, 991
MwoI GCNNNNNNNGC 1 cut(s) 1467
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1366
NdeII GATC 4 cut(s) 6, 382, 1408, 1525
NlaIV GGNNCC 5 cut(s) 329, 738, 925, 1362, 1390
NmuCI GTSAC 3 cut(s) 754, 1123, 1291
NspI RCATGY 1 cut(s) 239
NspV TTCGAA 2 cut(s) 74, 809
PagI TCATGA 1 cut(s) 838
PfeI GAWTC 4 cut(s) 199, 207, 637, 1235
PflMI CCANNNNNTGG 1 cut(s) 1288
PkrI GCNGC 2 cut(s) 277, 592
PleI GAGTC 3 cut(s) 448, 1030, 1066
PpsI GAGTC 3 cut(s) 448, 1030, 1066
PsiI TTATAA 1 cut(s) 911
PspN4I GGNNCC 5 cut(s) 329, 738, 925, 1362, 1390
PspPI GGNCC 3 cut(s) 328, 343, 938
PsuI RGATCY 2 cut(s) 6, 1408
PvuII CAGCTG 2 cut(s) 278, 593
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 3 cut(s) 1007, 1562, 1576
SaqAI TTAA 2 cut(s) 627, 1472
SatI GCNGC 2 cut(s) 276, 591
Sau3AI GATC 4 cut(s) 6, 382, 1408, 1525
Sau96I GGNCC 3 cut(s) 328, 343, 938
SchI GAGTC 3 cut(s) 449, 1031, 1067
SfaNI GCATC 2 cut(s) 948, 1566
SfuI TTCGAA 2 cut(s) 74, 809
SmiMI CAYNNNNRTG 3 cut(s) 1007, 1562, 1576
SmlI CTYRAG 1 cut(s) 697
SmoI CTYRAG 1 cut(s) 697
Sse9I AATT 9 cut(s) 52, 492, 912, 917, 967, 1134, 1260, 1399, 1489
SsiI CCGC 3 cut(s) 115, 324, 549
SspMI CTAG 2 cut(s) 587, 1575
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 1 cut(s) 760
TaiI ACGT 2 cut(s) 627, 985
TaqI TCGA 4 cut(s) 74, 405, 682, 809
TaqII GACCGA 1 cut(s) 387
TasI AATT 9 cut(s) 52, 492, 912, 917, 967, 1134, 1260, 1399, 1489
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 4 cut(s) 199, 207, 637, 1235
Tru1I TTAA 2 cut(s) 627, 1472
Tru9I TTAA 2 cut(s) 627, 1472
TscAI CASTG 2 cut(s) 1009, 1176
TseFI GTSAC 3 cut(s) 754, 1123, 1291
TseI GCWGC 2 cut(s) 275, 590
Tsp45I GTSAC 3 cut(s) 754, 1123, 1291
TspDTI ATGAA 7 cut(s) 99, 361, 485, 827, 855, 1496, 1563
TspRI CASTG 2 cut(s) 1009, 1176
Van91I CCANNNNNTGG 1 cut(s) 1288
XapI RAATTY 3 cut(s) 52, 492, 917
XbaI TCTAGA 1 cut(s) 1574
XceI RCATGY 1 cut(s) 239
XspI CTAG 2 cut(s) 587, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.