RchiOBHm_Chr5g0017691

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000925
Physical Location & Seq
Reverse (-)
12287156 .. 12290941
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29795

Sequence Viewer

Length: 993 bp
ATGAATTTTAGTCCTAGATTGATTTTGTGCAAGAGGCTCTCAAGCATTAAAAAATTGTGGAGATTCTTCACCTCATACTTGAAGAAATATCTGCTACAGCCTACAGATTGCTCAAAGTCACAATGTTTGTTCCTCTCACATGGCATATGCTTTAAAGTGAGTATAAAACACCTATCATTGAAAGCTGTATTTGCCAAGAACCTTCCCTTAGGGGAGAAGGCTCAAGCTTTGGAGTCATTCTTGCATGGAAACTTAAGACTGGTTCCAGTTCCCCCATCTGTAACCGTTTTCCAAATTGAAAAGACCATAGAAGAAGGTGCTACCAGACTTCTTTCTAAGTTGCCAGTCATAGCACTGGATCGAATCTGTTATGCACTATGCTGGCTTCTCAAAAGTGAACCTCTGGAAGTTTTGCTTAACCGGGCTCAAACGTCTAGGCACTTCTTCAAAGCAAAATCAGACTATGTGACCAAACCCATTTCAGAAGCTGGCTTGGAAGGTTTATGGAAAAGACTACTTCAAGTAGAGACATCCCAGTTGATACTGTCACCCTACGGTGGAAAGATGAGTGAGATTTCTGATTCAGAAACTCCATTCCCACATAGAAGAGGAAACATATTCAAAATCCAGTATTTGGTCACTTGGGAAGTTGGGATGATGAAAAAGGAGACAAACATACATATTGGCTGGATGAAGAAGCTGTATGCATATATGGCACCATATGTTTCCAAGTCCCCAAGAGCTGCCTATTTGAACTACAGGGATCTTGACTTGGGGAGGAACAACAATGATGGCAATACAAGCTATGCGAAAGCAAGCATTTGGGGTTTGAAGTATTTCAAGAGCAATTTCAGGAGACTTGTTCATGTTAAGATCTTGGTTGATCCTTGTAACTTCTTTAGAAATGAACAAAGCATCCCTGTGTTCCCATCTAGGGGAAAACAGACTTTCCAAATTAAATTGATTGGATTGGCCGAAAGAAGAAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

330

Amino Acids

38.06

Weight (kDa)

9.94

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BBE PF08031 249 - 307 9.1e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 715
AccB7I CCANNNNNTGG 1 cut(s) 634
AclWI GGATC 3 cut(s) 366, 771, 878
AcoI YGGCCR 1 cut(s) 972
AcsI RAATTY 1 cut(s) 4
AfiI CCNNNNNNNGG 4 cut(s) 557, 634, 934, 935
AflII CTTAAG 1 cut(s) 253
AgsI TTSAA 9 cut(s) 82, 181, 299, 448, 521, 622, 754, 832, 841
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
AluBI AGCT 6 cut(s) 185, 227, 488, 700, 743, 804
AluI AGCT 6 cut(s) 185, 227, 488, 700, 743, 804
Alw26I GTCTC 3 cut(s) 521, 662, 850
AlwI GGATC 3 cut(s) 366, 771, 878
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 1 cut(s) 972
ApeKI GCWGC 1 cut(s) 743
ApoI RAATTY 1 cut(s) 4
Asp700I GAANNNNTTC 1 cut(s) 836
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 61, 540
AxyI CCTNAGG 1 cut(s) 208
BanI GGYRCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 730
BccI CCATC 3 cut(s) 283, 785, 937
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 3 cut(s) 521, 662, 850
BfaI CTAG 3 cut(s) 15, 435, 933
BfmI CTRYAG 3 cut(s) 95, 102, 757
BfrI CTTAAG 1 cut(s) 253
BglII AGATCT 1 cut(s) 873
BisI GCNGC 1 cut(s) 744
BlsI GCNGC 1 cut(s) 745
Bme1390I CCNGG 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 264, 717
BmrFI CCNGG 1 cut(s) 422
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 1 cut(s) 924
BmuI ACTGGG 1 cut(s) 529
BpuEI CTTGAG 2 cut(s) 25, 207
BpuMI CCSGG 1 cut(s) 422
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 634, 934, 935
Bse1I ACTGG 6 cut(s) 264, 266, 344, 360, 535, 628
Bse21I CCTNAGG 1 cut(s) 208
BseGI GGATG 4 cut(s) 530, 660, 696, 915
BseLI CCNNNNNNNGG 4 cut(s) 557, 634, 934, 935
BseNI ACTGG 6 cut(s) 264, 266, 344, 360, 535, 628
BseXI GCAGC 1 cut(s) 730
BshFI GGCC 1 cut(s) 974
BshNI GGYRCC 1 cut(s) 715
BsiSI CCGG 1 cut(s) 421
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 4 cut(s) 557, 634, 934, 935
BsmAI GTCTC 3 cut(s) 521, 662, 850
BsmFI GGGAC 1 cut(s) 718
BsnI GGCC 1 cut(s) 974
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 4 cut(s) 358, 763, 873, 883
BspANI GGCC 1 cut(s) 974
BspLI GGNNCC 2 cut(s) 264, 717
BspPI GGATC 3 cut(s) 366, 771, 878
BspT107I GGYRCC 1 cut(s) 715
BspTI CTTAAG 1 cut(s) 253
BsrI ACTGG 6 cut(s) 264, 266, 344, 360, 535, 628
BssMI GATC 4 cut(s) 358, 763, 873, 883
Bst4CI ACNGT 3 cut(s) 286, 546, 557
Bst6I CTCTTC 1 cut(s) 601
BstAFI CTTAAG 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 383, 490, 817
BstDEI CTNAG 2 cut(s) 208, 336
BstF5I GGATG 4 cut(s) 530, 660, 696, 915
BstKTI GATC 4 cut(s) 361, 766, 876, 886
BstMAI GTCTC 3 cut(s) 521, 662, 850
BstMBI GATC 4 cut(s) 358, 763, 873, 883
BstMWI GCNNNNNNNGC 3 cut(s) 191, 713, 801
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 3 cut(s) 95, 102, 757
BstV1I GCAGC 1 cut(s) 730
BstX2I RGATCY 2 cut(s) 763, 873
BstYI RGATCY 2 cut(s) 763, 873
Bsu36I CCTNAGG 1 cut(s) 208
BsuRI GGCC 1 cut(s) 974
BtsCI GGATG 4 cut(s) 530, 660, 696, 915
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 383, 490, 817
CaiI CAGNNNCTG 1 cut(s) 488
CviAII CATG 3 cut(s) 140, 245, 866
DdeI CTNAG 2 cut(s) 208, 336
DpnI GATC 4 cut(s) 360, 765, 875, 885
DpnII GATC 4 cut(s) 358, 763, 873, 883
DraI TTTAAA 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 972
Eam1104I CTCTTC 1 cut(s) 601
EarI CTCTTC 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 427
Eco81I CCTNAGG 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 427
FaeI CATG 3 cut(s) 143, 248, 869
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 1 cut(s) 718
FatI CATG 3 cut(s) 139, 244, 865
FauNDI CATATG 2 cut(s) 146, 721
Fnu4HI GCNGC 1 cut(s) 744
FokI GGATG 4 cut(s) 517, 667, 703, 902
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 1 cut(s) 744
FspBI CTAG 3 cut(s) 15, 435, 933
GluI GCNGC 1 cut(s) 744
HaeIII GGCC 1 cut(s) 974
HapII CCGG 1 cut(s) 421
Hin1II CATG 3 cut(s) 143, 248, 869
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 4 cut(s) 63, 233, 363, 581
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 61, 540
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 4 cut(s) 460, 484, 580, 586
Hpy188III TCNNGA 4 cut(s) 404, 767, 841, 853
Hpy8I GTNNAC 1 cut(s) 398
HpyAV CCTTC 4 cut(s) 211, 212, 308, 491
HpyCH4III ACNGT 3 cut(s) 286, 546, 557
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 4 cut(s) 30, 244, 374, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 713, 801
HpyF3I CTNAG 2 cut(s) 208, 336
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 3 cut(s) 143, 248, 869
Kzo9I GATC 4 cut(s) 358, 763, 873, 883
Lsp1109I GCAGC 1 cut(s) 730
LweI GCATC 1 cut(s) 924
MaeI CTAG 3 cut(s) 15, 435, 933
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 6 cut(s) 117, 280, 466, 546, 637, 890
MalI GATC 4 cut(s) 360, 765, 875, 885
MboI GATC 4 cut(s) 358, 763, 873, 883
MboII GAAGA 7 cut(s) 58, 94, 323, 436, 618, 706, 993
MflI RGATCY 2 cut(s) 763, 873
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 6 cut(s) 4, 53, 294, 847, 954, 959
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 6 cut(s) 27, 82, 143, 411, 602, 771
Mph1103I ATGCAT 1 cut(s) 709
MroXI GAANNNNTTC 1 cut(s) 836
MseI TTAA 6 cut(s) 48, 153, 254, 417, 870, 957
MslI CAYNNNNRTG 1 cut(s) 920
MspCI CTTAAG 1 cut(s) 253
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 1 cut(s) 422
MwoI GCNNNNNNNGC 3 cut(s) 191, 713, 801
NciI CCSGG 1 cut(s) 422
NdeI CATATG 2 cut(s) 146, 721
NdeII GATC 4 cut(s) 358, 763, 873, 883
NlaIII CATG 3 cut(s) 143, 248, 869
NlaIV GGNNCC 2 cut(s) 264, 717
NmuCI GTSAC 4 cut(s) 117, 466, 546, 637
NsiI ATGCAT 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 3 cut(s) 63, 363, 581
PflMI CCANNNNNTGG 1 cut(s) 634
PkrI GCNGC 1 cut(s) 745
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 264, 717
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 2 cut(s) 763, 873
RseI CAYNNNNRTG 1 cut(s) 920
SaqAI TTAA 6 cut(s) 48, 153, 254, 417, 870, 957
SatI GCNGC 1 cut(s) 744
Sau3AI GATC 4 cut(s) 358, 763, 873, 883
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 1 cut(s) 924
SfcI CTRYAG 3 cut(s) 95, 102, 757
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 3 cut(s) 40, 222, 253
SmoI CTYRAG 3 cut(s) 40, 222, 253
Sse9I AATT 6 cut(s) 4, 53, 294, 847, 954, 959
SspMI CTAG 3 cut(s) 15, 435, 933
StyD4I CCNGG 1 cut(s) 420
TaaI ACNGT 3 cut(s) 286, 546, 557
TaiI ACGT 1 cut(s) 434
TaqI TCGA 1 cut(s) 361
TasI AATT 6 cut(s) 4, 53, 294, 847, 954, 959
TfiI GAWTC 3 cut(s) 63, 363, 581
Tru1I TTAA 6 cut(s) 48, 153, 254, 417, 870, 957
Tru9I TTAA 6 cut(s) 48, 153, 254, 417, 870, 957
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 4 cut(s) 117, 466, 546, 637
TseI GCWGC 1 cut(s) 743
Tsp45I GTSAC 4 cut(s) 117, 466, 546, 637
TspDTI ATGAA 5 cut(s) 17, 674, 707, 854, 921
TspRI CASTG 1 cut(s) 360
Van91I CCANNNNNTGG 1 cut(s) 634
Vha464I CTTAAG 1 cut(s) 253
XapI RAATTY 1 cut(s) 4
XmnI GAANNNNTTC 1 cut(s) 836
XspI CTAG 3 cut(s) 15, 435, 933
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.