MD05G1268000.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000925
Physical Location & Seq
Forward (+)
40315863 .. 40317455
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1268000.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGAGATCTCACACATAAAACTACTTCCCCTACTACTTCTCATCCTTCTAAGCTCGGTTTGCTCCACAACTTCTAACTCGATTTCGGAAAGCTTTGTTCAATGCTTTTCCTCCATCATACACTCTAACTCAACGAGCAGCATCATTCTCACCGAAAACAGTTCGGTTTATTCCTCAGTCTTGCAATCTTCCATACAAAATCTCAGATTCTTGAACACAACACCGAAACCAAAAGCTATCATTACCCCTTCTCAATACTCTCATGTTCAAGCAGCTATAGTATGCTCCAAGAAAGAGGGCATAGAAATAAGAACACGAAGCGGGGGCCATGACTATGAGGGCCTATCTTATGTATCCGAATCTCCTTTCATCATCATTGATCTTTTCAATCTTCGATCCATTGATATTGACATAAAGAAGGAGAGGGCTTGGGTTGAGTCAGGTGCTACTCTCGGAGAGTTGTACTACGCAATTGCACAAAAGAGCAAACTCTATGGCTTTCCAGCAGGTATCTGTCCAACTATTGGTGTGGGAGGACACATTAGTGGAGGTGGACATGGTCCCTTGTTCAGGAAATATGGTCTAGCAGCTGACAATGTCCTTGATGCTAAGATTGTCGACGTTAATGGCAGAGTTCTTGACAGAAAATCCATGGGAGAAGAGCTCTTTTGGGCTATTCGAGGAGGCGGAGGCTCAAGCTTTGGAGTCATTGTGGCTTGGAAACTCAGGTTGGTTCCTGTTCCTCCAACTGTAACAGGTTACACGGTATCGAAGACCATAGAACAAGGTACAACCAAACTTGTATCTAAATGGCAAGCTATAGCTGACAGGATGCCTGAAGAGTATTTCCTGCGTTTGGTTATCCAAGTTGCAAATGGAGCTGGTAAAAATGGTGGAAAAACTATACAAGTTTTATTCAATTTCTTGTTTTTCGAGAGTGTTGAGAAACTTCTTCCTTGGATGCAAGAAAATTTCCCGGAGTTAGATTTAGAGCGGAAGAATTTCATTGAGATGAGTTGGATTTCATCAGTTCTATACATTGCTAACATTCCAAGAAACGAAACAGAAGTTCTGCTTCAGAGGACTCAACAATCTAAGAGATTCTTCAAAGCCAAATCAGATTATGTTACAAAACCTATTTCAGGGGCTGCCTTGGAAGGTTTATGGAAAACAATGCTTGAATTGGATTCATTCCAGTTGATATTGACACCTTTCGGTGGAAGAATGAGTGAGATTTCTGATTCAGAAATTCCTTTCCCACATAGAAAAGGAAATTTGTTTGAGATCCAGTATTTCGACAATTGGGGTGATGCCGAAGAAACTGAGAAACATATTAGCGGGATGAGAATGGTGTATGCCTACCTGGCACCGTACGTTTCCAAGTCTCCAAGAGCTTCCTATTTAAACTATAGAGATCTTGACTTGGGAAGGAATGGTGATTGCAATACAAGCTATGCACAAGCAAGCGCTTGGGGTTTGAAGTATTTCAAGAATAACTTTAGGAGACTTGTTCAAGTTAAGACTCTAGTCGATCCCGGTAACTTCTTTAGAGATGAACAAACCATCCCTGTGTTACACTCCGGGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

531

Amino Acids

59.29

Weight (kDa)

8.79

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 77 - 213 6.3e-28 FAD binding domain
BBE PF08031 466 - 523 1.8e-16 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 1366
AccB7I CCANNNNNTGG 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 994
AccI GTMKAC 1 cut(s) 618
AciI CCGC 4 cut(s) 321, 687, 994, 1338
AclWI GGATC 3 cut(s) 390, 1279, 1526
AcsI RAATTY 4 cut(s) 970, 1000, 1248, 1273
AcuI CTGAAG 2 cut(s) 858, 1061
AfaI GTAC 3 cut(s) 464, 790, 1373
AfeI AGCGCT 1 cut(s) 1468
AfiI CCNNNNNNNGG 5 cut(s) 524, 570, 856, 1142, 1217
AjnI CCWGG 1 cut(s) 1362
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AleI CACNNNNGTG 1 cut(s) 543
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 2 cut(s) 1389, 1498
AlwI GGATC 3 cut(s) 390, 1279, 1526
AlwNI CAGNNNCTG 1 cut(s) 1148
Aor51HI AGCGCT 1 cut(s) 1468
AoxI GGCC 2 cut(s) 325, 340
ApeKI GCWGC 4 cut(s) 138, 272, 587, 1148
ApoI RAATTY 4 cut(s) 970, 1000, 1248, 1273
Asp700I GAANNNNTTC 2 cut(s) 1001, 1484
AspLEI GCGC 1 cut(s) 1469
AspS9I GGNCC 3 cut(s) 325, 340, 560
AsuC2I CCSGG 3 cut(s) 977, 1536, 1582
AsuHPI GGTGA 3 cut(s) 142, 1319, 1448
AvaII GGWCC 1 cut(s) 560
BanI GGYRCC 1 cut(s) 1366
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 779
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 4 cut(s) 150, 284, 599, 1135
BccI CCATC 2 cut(s) 122, 1571
BciT130I CCWGG 1 cut(s) 1364
BciVI GTATCC 1 cut(s) 364
BcnI CCSGG 3 cut(s) 977, 1536, 1582
BcoDI GTCTC 2 cut(s) 1389, 1498
BfaI CTAG 2 cut(s) 584, 1526
BfmI CTRYAG 3 cut(s) 276, 819, 1408
BfoI RGCGCY 1 cut(s) 1470
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 1 cut(s) 364
BglI GCCNNNNNGGC 1 cut(s) 1364
BglII AGATCT 2 cut(s) 6, 1414
BisI GCNGC 4 cut(s) 139, 273, 588, 1149
BlsI GCNGC 4 cut(s) 140, 274, 589, 1150
Bme1390I CCNGG 4 cut(s) 977, 1364, 1536, 1582
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 3 cut(s) 325, 340, 560
BmiI GGNNCC 4 cut(s) 326, 562, 735, 1368
BmrFI CCNGG 4 cut(s) 977, 1364, 1536, 1582
BmsI GCATC 5 cut(s) 150, 595, 822, 951, 1300
BoxI GACNNNNGTC 1 cut(s) 1526
BpiI GAAGAC 1 cut(s) 779
BplI GAGNNNNNCTC 2 cut(s) 648, 680
BpuEI CTTGAG 1 cut(s) 679
BpuMI CCSGG 3 cut(s) 977, 1536, 1582
BsaJI CCNNGG 3 cut(s) 651, 956, 1152
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 5 cut(s) 524, 570, 856, 1142, 1217
Bse1I ACTGG 2 cut(s) 1195, 1288
Bse3DI GCAATG 1 cut(s) 1038
BseBI CCWGG 1 cut(s) 1364
BseDI CCNNGG 3 cut(s) 651, 956, 1152
BseGI GGATG 5 cut(s) 42, 837, 966, 1347, 1563
BseLI CCNNNNNNNGG 5 cut(s) 524, 570, 856, 1142, 1217
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 4 cut(s) 189, 217, 739, 1314
BseNI ACTGG 2 cut(s) 1195, 1288
BseRI GAGGAG 1 cut(s) 696
BseXI GCAGC 4 cut(s) 150, 284, 599, 1135
BshFI GGCC 2 cut(s) 327, 342
BshNI GGYRCC 1 cut(s) 1366
BsiHKAI GWGCWC 1 cut(s) 666
BsiSI CCGG 3 cut(s) 977, 1536, 1581
BsiWI CGTACG 1 cut(s) 1371
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 5 cut(s) 524, 570, 856, 1142, 1217
BsmAI GTCTC 2 cut(s) 1389, 1498
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 2 cut(s) 327, 342
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 4 cut(s) 321, 687, 994, 1338
BspANI GGCC 2 cut(s) 327, 342
BspCNI CTCAG 4 cut(s) 188, 216, 738, 1315
BspLI GGNNCC 4 cut(s) 326, 562, 735, 1368
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 3 cut(s) 390, 1279, 1526
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 1366
BsrBI CCGCTC 1 cut(s) 994
BsrDI GCAATG 1 cut(s) 1038
BsrI ACTGG 2 cut(s) 1195, 1288
BssECI CCNNGG 3 cut(s) 651, 956, 1152
BssMI GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
BssT1I CCWWGG 3 cut(s) 651, 956, 1152
Bst2UI CCWGG 1 cut(s) 1364
Bst4CI ACNGT 4 cut(s) 161, 751, 766, 1371
Bst6I CTCTTC 2 cut(s) 654, 834
BstC8I GCNNGC 2 cut(s) 816, 1465
BstDEI CTNAG 7 cut(s) 50, 175, 203, 609, 725, 1095, 1323
BstDSI CCRYGG 1 cut(s) 651
BstENI CCTNNNNNAGG 2 cut(s) 568, 1140
BstF5I GGATG 5 cut(s) 42, 837, 966, 1347, 1563
BstH2I RGCGCY 1 cut(s) 1470
BstHHI GCGC 1 cut(s) 1469
BstKTI GATC 6 cut(s) 9, 382, 398, 1287, 1417, 1534
BstMAI GTCTC 2 cut(s) 1389, 1498
BstMBI GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
BstMWI GCNNNNNNNGC 4 cut(s) 60, 878, 1364, 1449
BstNI CCWGG 1 cut(s) 1364
BstPAI GACNNNNGTC 1 cut(s) 1526
BstSCI CCNGG 4 cut(s) 975, 1362, 1534, 1580
BstSFI CTRYAG 3 cut(s) 276, 819, 1408
BstV1I GCAGC 4 cut(s) 150, 284, 599, 1135
BstV2I GAAGAC 1 cut(s) 779
BstX2I RGATCY 3 cut(s) 6, 1284, 1414
BstYI RGATCY 3 cut(s) 6, 1284, 1414
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 327, 342
BtgI CCRYGG 1 cut(s) 651
BtsCI GGATG 5 cut(s) 42, 837, 966, 1347, 1563
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 2 cut(s) 816, 1465
CaiI CAGNNNCTG 1 cut(s) 1148
CfoI GCGC 1 cut(s) 1469
Cfr13I GGNCC 3 cut(s) 325, 340, 560
Csp6I GTAC 3 cut(s) 463, 789, 1372
CviAII CATG 4 cut(s) 263, 329, 557, 652
CviQI GTAC 3 cut(s) 463, 789, 1372
DdeI CTNAG 7 cut(s) 50, 175, 203, 609, 725, 1095, 1323
DpnI GATC 6 cut(s) 8, 381, 397, 1286, 1416, 1533
DpnII GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
DraI TTTAAA 1 cut(s) 1404
Eam1104I CTCTTC 2 cut(s) 654, 834
EarI CTCTTC 2 cut(s) 654, 834
EciI GGCGGA 1 cut(s) 702
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 3 cut(s) 651, 956, 1152
Eco24I GRGCYC 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 560
Eco47III AGCGCT 1 cut(s) 1468
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 2 cut(s) 858, 1061
EcoICRI GAGCTC 1 cut(s) 664
EcoNI CCTNNNNNAGG 2 cut(s) 568, 1140
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 1362
EcoT14I CCWWGG 3 cut(s) 651, 956, 1152
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 3 cut(s) 651, 956, 1152
FaeI CATG 4 cut(s) 266, 332, 560, 655
FalI AAGNNNNNCTT 6 cut(s) 1059, 1091, 1088, 1120, 1481, 1513
FaqI GGGAC 1 cut(s) 546
FatI CATG 4 cut(s) 262, 328, 556, 651
FauI CCCGC 2 cut(s) 314, 1331
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 4 cut(s) 139, 273, 588, 1149
FokI GGATG 5 cut(s) 29, 844, 973, 1354, 1550
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 4 cut(s) 139, 273, 588, 1149
FspBI CTAG 2 cut(s) 584, 1526
GlaI GCGC 1 cut(s) 1468
GluI GCNGC 4 cut(s) 139, 273, 588, 1149
HaeII RGCGCY 1 cut(s) 1470
HaeIII GGCC 2 cut(s) 327, 342
HapII CCGG 3 cut(s) 977, 1536, 1581
HhaI GCGC 1 cut(s) 1469
Hin1II CATG 4 cut(s) 266, 332, 560, 655
Hin6I GCGC 1 cut(s) 1467
HinP1I GCGC 1 cut(s) 1467
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 2 cut(s) 91, 697
HinfI GANTC 9 cut(s) 207, 359, 437, 705, 1084, 1101, 1187, 1241, 1522
HpaII CCGG 3 cut(s) 977, 1536, 1581
HphI GGTGA 3 cut(s) 142, 1319, 1448
Hpy166II GTNNAC 2 cut(s) 554, 619
Hpy188I TCNGA 8 cut(s) 88, 206, 358, 455, 1080, 1120, 1240, 1246
Hpy188III TCNNGA 6 cut(s) 211, 571, 638, 934, 1418, 1489
Hpy8I GTNNAC 2 cut(s) 554, 619
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 5 cut(s) 56, 258, 412, 1151, 1422
HpyCH4III ACNGT 4 cut(s) 161, 751, 766, 1371
HpyCH4IV ACGT 2 cut(s) 621, 1374
HpyCH4V TGCA 6 cut(s) 184, 476, 872, 964, 1443, 1457
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 878, 1364, 1449
HpyF3I CTNAG 7 cut(s) 50, 175, 203, 609, 725, 1095, 1323
HpySE526I ACGT 2 cut(s) 621, 1374
Hsp92II CATG 4 cut(s) 266, 332, 560, 655
HspAI GCGC 1 cut(s) 1467
Kzo9I GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 3 cut(s) 68, 290, 878
Lsp1109I GCAGC 4 cut(s) 150, 284, 599, 1135
LweI GCATC 5 cut(s) 150, 595, 822, 951, 1300
MaeI CTAG 2 cut(s) 584, 1526
MaeII ACGT 2 cut(s) 621, 1374
MaeIII GTNAC 5 cut(s) 751, 758, 1126, 1538, 1572
MalI GATC 6 cut(s) 8, 381, 397, 1286, 1416, 1533
MbiI CCGCTC 1 cut(s) 994
MboI GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
MfeI CAATTG 2 cut(s) 471, 1300
MflI RGATCY 3 cut(s) 6, 1284, 1414
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 8 cut(s) 471, 919, 970, 1000, 1181, 1248, 1273, 1300
MlyI GAGTC 4 cut(s) 446, 714, 1078, 1516
MmeI TCCRAC 3 cut(s) 542, 770, 998
MroXI GAANNNNTTC 2 cut(s) 1001, 1484
MseI TTAA 3 cut(s) 624, 1403, 1518
MslI CAYNNNNRTG 4 cut(s) 333, 543, 1010, 1568
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 3 cut(s) 977, 1536, 1581
MspR9I CCNGG 4 cut(s) 977, 1364, 1536, 1582
MunI CAATTG 2 cut(s) 471, 1300
MvaI CCWGG 1 cut(s) 1364
MwoI GCNNNNNNNGC 4 cut(s) 60, 878, 1364, 1449
NciI CCSGG 3 cut(s) 977, 1536, 1582
NcoI CCATGG 1 cut(s) 651
NdeII GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
NlaIII CATG 4 cut(s) 266, 332, 560, 655
NlaIV GGNNCC 4 cut(s) 326, 562, 735, 1368
OliI CACNNNNGTG 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 654
PdmI GAANNNNTTC 2 cut(s) 1001, 1484
PfeI GAWTC 5 cut(s) 207, 359, 1101, 1187, 1241
Pfl23II CGTACG 1 cut(s) 1371
PflFI GACNNNGTC 2 cut(s) 558, 596
PflMI CCANNNNNTGG 1 cut(s) 524
PfoI TCCNGGA 2 cut(s) 975, 1580
PkrI GCNGC 4 cut(s) 140, 274, 589, 1150
PleI GAGTC 4 cut(s) 445, 713, 1078, 1516
PpsI GAGTC 4 cut(s) 445, 713, 1078, 1516
PshAI GACNNNNGTC 1 cut(s) 1526
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 1362
PspGI CCWGG 1 cut(s) 1362
PspLI CGTACG 1 cut(s) 1371
PspN4I GGNNCC 4 cut(s) 326, 562, 735, 1368
PspPI GGNCC 3 cut(s) 325, 340, 560
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 3 cut(s) 6, 1284, 1414
PsyI GACNNNGTC 2 cut(s) 558, 596
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 3 cut(s) 464, 790, 1373
RsaNI GTAC 3 cut(s) 463, 789, 1372
RseI CAYNNNNRTG 4 cut(s) 333, 543, 1010, 1568
SacI GAGCTC 1 cut(s) 666
SalI GTCGAC 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 3 cut(s) 624, 1403, 1518
SatI GCNGC 4 cut(s) 139, 273, 588, 1149
Sau3AI GATC 6 cut(s) 6, 379, 395, 1284, 1414, 1531
Sau96I GGNCC 3 cut(s) 325, 340, 560
SchI GAGTC 4 cut(s) 446, 714, 1078, 1516
ScrFI CCNGG 4 cut(s) 977, 1364, 1536, 1582
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 5 cut(s) 150, 595, 822, 951, 1300
SfcI CTRYAG 3 cut(s) 276, 819, 1408
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 4 cut(s) 333, 543, 1010, 1568
SmlI CTYRAG 1 cut(s) 694
SmoI CTYRAG 1 cut(s) 694
Sse9I AATT 8 cut(s) 471, 919, 970, 1000, 1181, 1248, 1273, 1300
SsiI CCGC 4 cut(s) 321, 687, 994, 1338
SspMI CTAG 2 cut(s) 584, 1526
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 4 cut(s) 975, 1362, 1534, 1580
StyI CCWWGG 3 cut(s) 651, 956, 1152
TaaI ACNGT 4 cut(s) 161, 751, 766, 1371
TaiI ACGT 2 cut(s) 624, 1377
TaqI TCGA 8 cut(s) 80, 394, 618, 679, 770, 933, 1296, 1530
TasI AATT 8 cut(s) 471, 919, 970, 1000, 1181, 1248, 1273, 1300
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 5 cut(s) 207, 359, 1101, 1187, 1241
Tru1I TTAA 3 cut(s) 624, 1403, 1518
Tru9I TTAA 3 cut(s) 624, 1403, 1518
TseI GCWGC 4 cut(s) 138, 272, 587, 1148
TspDTI ATGAA 5 cut(s) 358, 994, 1014, 1179, 1569
Tth111I GACNNNGTC 2 cut(s) 558, 596
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 560
XagI CCTNNNNNAGG 2 cut(s) 568, 1140
XapI RAATTY 4 cut(s) 970, 1000, 1248, 1273
XcmI CCANNNNNNNNNTGG 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 2 cut(s) 1001, 1484
XspI CTAG 2 cut(s) 584, 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.