RchiOBHm_Chr4g0431081

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000925
Physical Location & Seq
Reverse (-)
55406755 .. 55407871
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39979

Sequence Viewer

Length: 897 bp
ATGGAGACCTTTCCTGCCATCATTACTCCTTTTGATGAATCTCAGGTTCAAGCAGCTGTAATTTGCTCCAAGAAAAGTGGCATACAAATAAGAATCCGAAGCCGGGGTCATGATTACGAGGGCCTATCTTATGTGTCTAGAGCTCCTTTCATCATCATTGACCTTTTCAATCTCCGTTCCATCGATGTTGATATAAAGAATGAGAGTGCTTGGGTCAAATCAGGCGCTACTCTTGGAGAGTTGTATTACAACATTGCACAGAAGAGTAAAGTCTATGCCTTTCCAGCAGGGACTTGTCCCACCGTTGGTGCTGGGGGACACATCAGTGGAGGTGGAGTTGGTACCATATTCAGAAAATATGGTCTAGCATCTGACAATGTCATTGATGCTAGAATTGTAGATCTTAATGGCAGAATTCTAGACAGAAAGTCCATGGGAGAAGAGCTCTTTTGGGCTATCAGAGGAGGAGGATCAAGCTTTGGAGTCATTCTAGCATGGAAACTTAGACTGGTTCCTGTTCCCCCAACTGTAACAGTTTGCGACGTTACAAAGACAAAAGAACAAGGTGCAACCAAATTTCTTTTGAAATGGCAAAACATTGCAGACAAGCTTCCTGAAGACCTTTTCCTACATTCAGTTGTAGGAAGTAGTGACAACACTATTACAGTTGAATTCGGTTCCTTGTTTCTCGGGCGGGTCGACAAAGCTGTCCAGGTAATACAGGAAAACTTCCCAGAGTTGAGTTTAGAGAGCAGCCATTGCAGTGAGATGAGTTGGATTCAGTCTGTTTTGGACTCTGCTGACTGCTGGCTACTCGATCCACGAATCCTTGGAGATTTTGCTGAAGCGCCGGCAATTCAGCTACTTCTTCAAAGCAAAATCAGACTTGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

298

Amino Acids

32.63

Weight (kDa)

5.67

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 6 - 140 9.2e-24 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 707
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 341
AccI GTMKAC 1 cut(s) 699
AciI CCGC 1 cut(s) 694
AclWI GGATC 2 cut(s) 478, 812
AcsI RAATTY 3 cut(s) 414, 575, 671
AcuI CTGAAG 2 cut(s) 636, 864
AfaI GTAC 1 cut(s) 343
AfiI CCNNNNNNNGG 2 cut(s) 103, 305
AgsI TTSAA 5 cut(s) 50, 169, 586, 671, 872
AhdI GACNNNNNGTC 1 cut(s) 427
AjnI CCWGG 1 cut(s) 711
AleI CACNNNNGTG 1 cut(s) 324
AluBI AGCT 7 cut(s) 56, 143, 445, 477, 610, 707, 862
AluI AGCT 7 cut(s) 56, 143, 445, 477, 610, 707, 862
Alw21I GWGCWC 2 cut(s) 145, 447
AlwI GGATC 2 cut(s) 478, 812
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 1 cut(s) 121
ApeKI GCWGC 2 cut(s) 53, 753
ApoI RAATTY 3 cut(s) 414, 575, 671
ArsI GACNNNNNNTTYG 2 cut(s) 91, 123
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 2 cut(s) 227, 850
AspS9I GGNCC 1 cut(s) 121
AsuC2I CCSGG 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 689
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 2 cut(s) 145, 447
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 145, 447
BbvI GCAGC 2 cut(s) 65, 765
BccI CCATC 2 cut(s) 26, 188
BciT130I CCWGG 1 cut(s) 713
BcnI CCSGG 1 cut(s) 104
BfaI CTAG 5 cut(s) 138, 365, 390, 419, 491
BfoI RGCGCY 2 cut(s) 228, 851
BglII AGATCT 1 cut(s) 400
BisI GCNGC 2 cut(s) 54, 754
BlsI GCNGC 2 cut(s) 55, 755
Bme1390I CCNGG 2 cut(s) 104, 713
BmeRI GACNNNNNGTC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 3 cut(s) 343, 513, 679
BmrFI CCNGG 2 cut(s) 104, 713
BmsI GCATC 2 cut(s) 376, 377
BpiI GAAGAC 1 cut(s) 624
BplI GAGNNNNNCTC 2 cut(s) 429, 461
BpuMI CCSGG 1 cut(s) 104
Bsa29I ATCGAT 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 103, 432, 829
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 305
Bse118I RCCGGY 1 cut(s) 850
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 3 cut(s) 252, 597, 757
BseBI CCWGG 1 cut(s) 713
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 3 cut(s) 103, 432, 829
BseLI CCNNNNNNNGG 2 cut(s) 103, 305
BseMI GCAATG 3 cut(s) 252, 597, 757
BseMII CTCAG 1 cut(s) 56
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 2 cut(s) 477, 480
BseXI GCAGC 2 cut(s) 65, 765
BseYI CCCAGC 1 cut(s) 311
BshFI GGCC 1 cut(s) 123
BshNI GGYRCC 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 183
BsiHKAI GWGCWC 2 cut(s) 145, 447
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 2 cut(s) 103, 851
BslFI GGGAC 3 cut(s) 282, 304, 330
BslI CCNNNNNNNGG 2 cut(s) 103, 305
BsmFI GGGAC 3 cut(s) 282, 304, 330
BsnI GGCC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 145, 447
Bsp143I GATC 3 cut(s) 400, 470, 817
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 1 cut(s) 694
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 55
BspDI ATCGAT 1 cut(s) 183
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 343, 513, 679
BspPI GGATC 2 cut(s) 478, 812
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 341
BsrDI GCAATG 3 cut(s) 252, 597, 757
BsrFI RCCGGY 1 cut(s) 850
BsrI ACTGG 1 cut(s) 513
BssAI RCCGGY 1 cut(s) 850
BssECI CCNNGG 3 cut(s) 103, 432, 829
BssMI GATC 3 cut(s) 400, 470, 817
BssT1I CCWWGG 2 cut(s) 432, 829
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 4 cut(s) 304, 529, 535, 667
Bst6I CTCTTC 2 cut(s) 257, 435
BstAPI GCANNNNNTGC 1 cut(s) 759
BstC8I GCNNGC 2 cut(s) 809, 852
BstDEI CTNAG 2 cut(s) 42, 503
BstDSI CCRYGG 1 cut(s) 432
BstH2I RGCGCY 2 cut(s) 228, 851
BstHHI GCGC 2 cut(s) 227, 850
BstKTI GATC 3 cut(s) 403, 473, 820
BstMBI GATC 3 cut(s) 400, 470, 817
BstMWI GCNNNNNNNGC 2 cut(s) 284, 759
BstNI CCWGG 1 cut(s) 713
BstSCI CCNGG 2 cut(s) 102, 711
BstV1I GCAGC 2 cut(s) 65, 765
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 1 cut(s) 123
BsuTUI ATCGAT 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 432
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 2 cut(s) 331, 769
Cac8I GCNNGC 2 cut(s) 809, 852
CciI TCATGA 1 cut(s) 109
CfoI GCGC 2 cut(s) 227, 850
Cfr10I RCCGGY 1 cut(s) 850
Cfr13I GGNCC 1 cut(s) 121
ClaI ATCGAT 1 cut(s) 183
Csp6I GTAC 1 cut(s) 342
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 3 cut(s) 110, 433, 495
CviQI GTAC 1 cut(s) 342
DdeI CTNAG 2 cut(s) 42, 503
DpnI GATC 3 cut(s) 402, 472, 819
DpnII GATC 3 cut(s) 400, 470, 817
DrdI GACNNNNNNGTC 1 cut(s) 707
DriI GACNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 707
Eam1104I CTCTTC 2 cut(s) 257, 435
Eam1105I GACNNNNNGTC 1 cut(s) 427
EarI CTCTTC 2 cut(s) 257, 435
Ecl136II GAGCTC 2 cut(s) 143, 445
Eco130I CCWWGG 2 cut(s) 432, 829
Eco24I GRGCYC 2 cut(s) 145, 447
Eco53kI GAGCTC 2 cut(s) 143, 445
Eco57I CTGAAG 2 cut(s) 636, 864
Eco88I CYCGRG 1 cut(s) 689
EcoICRI GAGCTC 2 cut(s) 143, 445
EcoO109I RGGNCCY 1 cut(s) 121
EcoRI GAATTC 2 cut(s) 414, 671
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 2 cut(s) 432, 829
EcoT38I GRGCYC 2 cut(s) 145, 447
ErhI CCWWGG 2 cut(s) 432, 829
FaeI CATG 3 cut(s) 113, 436, 498
FaiI YATR 9 cut(s) 83, 111, 132, 194, 276, 347, 360, 434, 496
FaqI GGGAC 3 cut(s) 282, 304, 330
FatI CATG 3 cut(s) 109, 432, 494
FauI CCCGC 1 cut(s) 687
FblI GTMKAC 1 cut(s) 699
Fnu4HI GCNGC 2 cut(s) 54, 754
FriOI GRGCYC 2 cut(s) 145, 447
Fsp4HI GCNGC 2 cut(s) 54, 754
FspBI CTAG 5 cut(s) 138, 365, 390, 419, 491
GlaI GCGC 2 cut(s) 226, 849
GluI GCNGC 2 cut(s) 54, 754
GsaI CCCAGC 1 cut(s) 315
HaeII RGCGCY 2 cut(s) 228, 851
HaeIII GGCC 1 cut(s) 123
HapII CCGG 2 cut(s) 103, 851
HhaI GCGC 2 cut(s) 227, 850
Hin1II CATG 3 cut(s) 113, 436, 498
Hin6I GCGC 2 cut(s) 225, 848
HinP1I GCGC 2 cut(s) 225, 848
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HindIII AAGCTT 2 cut(s) 475, 608
HinfI GANTC 6 cut(s) 38, 93, 483, 778, 794, 825
HpaII CCGG 2 cut(s) 103, 851
Hpy166II GTNNAC 1 cut(s) 700
Hpy188I TCNGA 5 cut(s) 98, 353, 373, 461, 884
Hpy188III TCNNGA 4 cut(s) 110, 138, 419, 614
Hpy8I GTNNAC 1 cut(s) 700
Hpy99I CGWCG 1 cut(s) 545
HpyCH4III ACNGT 4 cut(s) 304, 529, 535, 667
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 4 cut(s) 257, 569, 602, 762
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 759
HpyF3I CTNAG 2 cut(s) 42, 503
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 3 cut(s) 113, 436, 498
HspAI GCGC 2 cut(s) 225, 848
KpnI GGTACC 1 cut(s) 345
KroI GCCGGC 1 cut(s) 850
KroNI GCCGGC 1 cut(s) 852
Kzo9I GATC 3 cut(s) 400, 470, 817
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 2 cut(s) 71, 148
Lsp1109I GCAGC 2 cut(s) 65, 765
LweI GCATC 2 cut(s) 376, 377
MaeI CTAG 5 cut(s) 138, 365, 390, 419, 491
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 529, 544, 650, 890
MalI GATC 3 cut(s) 402, 472, 819
MboI GATC 3 cut(s) 400, 470, 817
MboII GAAGA 4 cut(s) 274, 452, 629, 860
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 145, 447
MluCI AATT 6 cut(s) 60, 393, 414, 575, 671, 855
MlyI GAGTC 2 cut(s) 492, 788
MmeI TCCRAC 1 cut(s) 755
MnlI CCTC 5 cut(s) 112, 323, 455, 458, 461
MroNI GCCGGC 1 cut(s) 850
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 2 cut(s) 324, 762
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 103, 851
MspR9I CCNGG 2 cut(s) 104, 713
MvaI CCWGG 1 cut(s) 713
MwoI GCNNNNNNNGC 2 cut(s) 284, 759
NaeI GCCGGC 1 cut(s) 852
NciI CCSGG 1 cut(s) 104
NcoI CCATGG 1 cut(s) 432
NdeII GATC 3 cut(s) 400, 470, 817
NgoMIV GCCGGC 1 cut(s) 850
NlaIII CATG 3 cut(s) 113, 436, 498
NlaIV GGNNCC 3 cut(s) 343, 513, 679
NmuCI GTSAC 2 cut(s) 650, 890
OliI CACNNNNGTG 1 cut(s) 324
PagI TCATGA 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PdiI GCCGGC 1 cut(s) 852
PfeI GAWTC 4 cut(s) 38, 93, 778, 825
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 2 cut(s) 55, 755
PleI GAGTC 2 cut(s) 491, 788
PpsI GAGTC 2 cut(s) 491, 788
Psp124BI GAGCTC 2 cut(s) 145, 447
Psp6I CCWGG 1 cut(s) 711
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 3 cut(s) 343, 513, 679
PspPI GGNCC 1 cut(s) 121
PsuI RGATCY 1 cut(s) 400
PsyI GACNNNGTC 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 56
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 2 cut(s) 324, 762
SacI GAGCTC 2 cut(s) 145, 447
SalI GTCGAC 1 cut(s) 698
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 2 cut(s) 54, 754
Sau3AI GATC 3 cut(s) 400, 470, 817
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 2 cut(s) 492, 788
ScrFI CCNGG 2 cut(s) 104, 713
SduI GDGCHC 2 cut(s) 145, 447
SfaNI GCATC 2 cut(s) 376, 377
SmiMI CAYNNNNRTG 2 cut(s) 324, 762
Sse9I AATT 6 cut(s) 60, 393, 414, 575, 671, 855
SsiI CCGC 1 cut(s) 694
SspMI CTAG 5 cut(s) 138, 365, 390, 419, 491
SstI GAGCTC 2 cut(s) 145, 447
StyD4I CCNGG 2 cut(s) 102, 711
StyI CCWWGG 2 cut(s) 432, 829
TaaI ACNGT 4 cut(s) 304, 529, 535, 667
TaiI ACGT 1 cut(s) 546
TaqI TCGA 3 cut(s) 183, 699, 816
TasI AATT 6 cut(s) 60, 393, 414, 575, 671, 855
TfiI GAWTC 4 cut(s) 38, 93, 778, 825
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 2 cut(s) 331, 769
TseFI GTSAC 2 cut(s) 650, 890
TseI GCWGC 2 cut(s) 53, 753
Tsp45I GTSAC 2 cut(s) 650, 890
TspDTI ATGAA 2 cut(s) 51, 139
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 2 cut(s) 331, 769
Tth111I GACNNNGTC 1 cut(s) 377
XapI RAATTY 3 cut(s) 414, 575, 671
XbaI TCTAGA 2 cut(s) 137, 418
XmiI GTMKAC 1 cut(s) 699
XspI CTAG 5 cut(s) 138, 365, 390, 419, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.